CmoCh18G006350 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh18G006350
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Description4-coumarate--CoA ligase-like 1
LocationCmo_Chr18: 8064232 .. 8066630 (-)
RNA-Seq ExpressionCmoCh18G006350
SyntenyCmoCh18G006350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGGCATGAATCAACAACATCCCACCAAAAAAAGGCTCATGCCAATCTCACCCTCAGTAGCTCTACAAATTTCTCATCCACAAAGCATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTTGGTAGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCTGAGGTCCAAGTGCCTGATGATATCACATTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTAAAATCGAAACTGTCGTTATCGATCATATTATTTCGAATTCAACTCGACTTGCTACTATCTAATGATTGATAACAGGTAAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACAAACTGCTCGAAGCTGCGGACCGAGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCTAACTTGTGTTCTACGCTCTCGGGCGTGCCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGTAATTAAGTTACTACTCGAGTAGATGGATTGTGAGAACTCTCGACTCTTATTCATTCTGTCAAATTATCAAGCTATAACATGTAGTCCACCTTCCTGTTTTCTGCATATCGAAAACGAACCGAGCTCCACCGTCGCATTGTAGAGCTGATTCACAGGGTCGTCCTACCTAAGCTAACTAAATGTCATTGATCACCAACAGGCATATGGGCTTACCGAGCACAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCGCTGCAAAGAAAAACACAGTCGGTCGCATTCTTCCTAATCTAGAAGTCAAATTCATTGACCCCGACAGCGGCAGGTCGCTGCCGAAGAACACGCCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTAATGCAAGGTACATAATTTGGACTGGTGCAGAAAATCCATCTGAGATCTCTAATTATATGCTAGTTGAATCTTTCCTTGTTTCAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAAAGACACTGCATAAACTTTGCTTGATAAATCGAAGCTCGAGAACAGAAAAAAAGTAGAATTCAAATGGTTATCAAACGAGACCTATATAAGGTAACGAATGTCTTTGATTTCTCTACAGGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGTATGCTTCGCTTCCCTTTACATATTTCTATTTAAATGATTTCTGAAACAAATCGAACCCGAAACGGTTAAAACAACGTTAACCCGCGTCAGTATTTCAGGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGGCTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGGCGGCCTCTTGA

mRNA sequence

ATGAGTGGCATGAATCAACAACATCCCACCAAAAAAAGGCTCATGCCAATCTCACCCTCAGTAGCTCTACAAATTTCTCATCCACAAAGCATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTTGGTAGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCTGAGGTCCAAGTGCCTGATGATATCACATTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTAAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACAAACTGCTCGAAGCTGCGGACCGAGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCTAACTTGTGTTCTACGCTCTCGGGCGTGCCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGGCTTACCGAGCACAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCGCTGCAAAGAAAAACACAGTCGGTCGCATTCTTCCTAATCTAGAAGTCAAATTCATTGACCCCGACAGCGGCAGGTCGCTGCCGAAGAACACGCCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTAATGCAAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGGCTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGGCGGCCTCTTGA

Coding sequence (CDS)

ATGAGTGGCATGAATCAACAACATCCCACCAAAAAAAGGCTCATGCCAATCTCACCCTCAGTAGCTCTACAAATTTCTCATCCACAAAGCATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTTGGTAGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCTGAGGTCCAAGTGCCTGATGATATCACATTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTAAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACAAACTGCTCGAAGCTGCGGACCGAGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCTAACTTGTGTTCTACGCTCTCGGGCGTGCCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGGCTTACCGAGCACAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCGCTGCAAAGAAAAACACAGTCGGTCGCATTCTTCCTAATCTAGAAGTCAAATTCATTGACCCCGACAGCGGCAGGTCGCTGCCGAAGAACACGCCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTAATGCAAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGGCTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGGCGGCCTCTTGA

Protein sequence

MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS
Homology
BLAST of CmoCh18G006350 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.2e-226
Identity = 389/532 (73.12%), Postives = 459/532 (86.28%), Query Frame = 0

Query: 66  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRF 125
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 126 AKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 185
           AKAL+SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 186 KLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 245
           + ++T+++++EKVK L LPVIV+GEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 246 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 305
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 306 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAI 365
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 366 MTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILP 425
           MTAAAPLAPEL T+FE KFP V VQEAYGLTEHSCITL +G   K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 426 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYID 485
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID++GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 486 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM 545
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPD+EAGEIPAA +V+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 546 APNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMI 598
            P A E E++I+ +VA+NVAHYKKVR VHFV++IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of CmoCh18G006350 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 702.2 bits (1811), Expect = 5.1e-201
Identity = 348/542 (64.21%), Postives = 441/542 (81.37%), Query Frame = 0

Query: 66  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVS-GKAYTYREVVRDTSR 125
           E++EH+FRS+ P V VPD +T+PEFVL  AE+YAD VA VEA + G++YTY EV RDT+R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 126 FAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAD 185
           FA+AL S+ ++KG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE ++
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 186 AKLVVTNSSSFEKVKELKLPVIVVGE-ELIEGSMNWHKLLEAADRAGNNFVKED-IKQTD 245
           AKLVV N  +F+KVK+  +PVI VG+ E + G+++W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 246 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGI 305
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 306 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKL 365
           CCATLR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+  EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 366 QAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGR 425
           +++MTAAAPLAP+L  +F++KFPGV V+EAYGLTEHSCITL + +       AKK++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 426 ILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIG 485
           ILPNLEVKF+DPD+GRSLP NTPGE+CVRSQ VMQGYY  +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 486 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAAS 545
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PD+EAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 546 IVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRA 605
           +V    A+E E+EI+ YVA  VA YK+VR++H V+ IPKS SGK++RR ++++ I++++ 
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMKP 553

BLAST of CmoCh18G006350 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.6e-112
Identity = 208/534 (38.95%), Postives = 331/534 (61.99%), Query Frame = 0

Query: 68  EEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAK 127
           E+ IFRS+LP++ +P  + L  +  +N     D    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 128 ALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 187
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 188 VVTNSSSFEKVKEL---KLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLC 247
           ++T +   +KVK+    K   I+  ++  +  +++ KL+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---INSDDVV 188

Query: 248 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGI 307
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 308 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKL 367
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V ++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 368 QAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGR 427
             +M+ AAPL  EL+ +   KFP   + + YG+TE   +     +  K+    K    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 428 ILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIG 487
           ++ N E+K +DP++  SLP+N  GEIC+R   +M+GY N+ E T  TID++GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 488 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAAS 547
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ D++AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 548 IVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
           +V       TE+EI ++V+  V  YK++  V FV+ IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of CmoCh18G006350 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.6e-112
Identity = 206/532 (38.72%), Postives = 330/532 (62.03%), Query Frame = 0

Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRS+LP++ +P  + L  +  +N   +      ++  + + YTY EV   + + A  L+
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 191 NSSSFEKVK----ELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCAL 250
            +    KVK    E  L VI V + + EG +++ +L+++ +   +      I+  D+ AL
Sbjct: 134 QACFAGKVKDYAIENDLKVICV-DSVPEGCVHFSELIQSDE---HEIPDVKIQPDDVVAL 193

Query: 251 PFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGICC 310
           P+SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY +  +  
Sbjct: 194 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLL 253

Query: 311 ATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQA 370
             LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V  +DLS ++   
Sbjct: 254 CALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVR--T 313

Query: 371 IMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRIL 430
           +M+ AAPL  EL+ +   KFP   + + YG+TE   +     +  K+    K    G ++
Sbjct: 314 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVV 373

Query: 431 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYI 490
            N E+K +DPD+G SLP+N PGEIC+R   +M+GY N+ E T+RTI+ +GW+HTGDIG+I
Sbjct: 374 RNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFI 433

Query: 491 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIV 550
           DDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ D++AGE+P A +V
Sbjct: 434 DDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVV 493

Query: 551 MAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
            +  +  TEDE+  +++  V  YK+++ V FVET+PKSPSGK++R+ ++ ++
Sbjct: 494 RSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of CmoCh18G006350 vs. ExPASy Swiss-Prot
Match: A0A2H5AIX5 (4-coumarate-CoA ligase 1 OS=Narcissus pseudonarcissus OX=39639 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 4.4e-112
Identity = 214/536 (39.93%), Postives = 330/536 (61.57%), Query Frame = 0

Query: 67  DEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFA 126
           ++E IFRS+LP++ VPD + L  +  QN   ++D    ++  + K  TY EV   + R  
Sbjct: 7   EKETIFRSKLPDIYVPDHLPLHSYCFQNLHQFSDRPCLIDGFTNKTLTYAEVELASKRVG 66

Query: 127 KALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAK 186
             L  L L++G VV+++LPN  E+ +  +G    G + +  NP    +EI KQ   A AK
Sbjct: 67  AGLHRLGLRQGHVVMLLLPNSIEFVLSFIGASLLGAMSTTANPFYTSAEIHKQAAAAGAK 126

Query: 187 LVVTNSSSFEKVKELK-LPVIVVGEELI---EGSMNWHKLLEAADRAGNNFVKEDIKQTD 246
           ++VT S    K++ L+ +  IVV ++ +   E  M++ + LE+ D A     + D+   D
Sbjct: 127 IIVTESCHVSKLQGLEGISRIVVIDDAVRVPENVMHFSE-LESTDEA--ELPRIDVHPDD 186

Query: 247 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--VGKVTTLGLIPFFHIYGIT 306
           + ALP+SSGTTG+ KGVMLTH  LV ++   + G    +        L ++P FHIY + 
Sbjct: 187 VVALPYSSGTTGLPKGVMLTHNGLVTSVAQQVDGENPNLHFSEDDVLLCVLPLFHIYSLN 246

Query: 307 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 366
            +    LR    +V+M +F++   +  +    VT AP VPPI++ +VKN  V  +DLS +
Sbjct: 247 SVLLCGLRAGAAIVLMRKFEIVRLMELVEKYRVTIAPFVPPIVVEMVKNEAVDRYDLSSI 306

Query: 367 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 426
           ++  +M+ AAP+  EL+    +K P   + + YG+TE   +     +  K+    K  + 
Sbjct: 307 RV--VMSGAAPMGKELENKLREKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGSC 366

Query: 427 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 486
           G ++ N E+K IDP++G SL +N PGEIC+R   +M+GY NN E T +TID++GW+HTGD
Sbjct: 367 GTVVRNAELKIIDPETGFSLSRNQPGEICIRGNQIMKGYLNNPEATKQTIDEEGWLHTGD 426

Query: 487 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 546
           IG++DDD ++FIVDR+KELIKYKGFQVAPAELEA+L+THP++ DAAVV + D   GE+P 
Sbjct: 427 IGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLITHPNMADAAVVSIKDDSCGELPV 486

Query: 547 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
           A IV +  ++ TEDEI KY++  V  YK++  V F+E IPK+PSGK++R+ ++ ++
Sbjct: 487 AFIVRSNGSEITEDEIKKYISKQVVFYKRIHRVFFIEAIPKAPSGKILRKELRARL 537

BLAST of CmoCh18G006350 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1175.6 bits (3040), Expect = 0.0e+00
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 0

Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60

Query: 61  IRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR
Sbjct: 61  IRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAEAAS 607
           RAEAAS
Sbjct: 601 RAEAAS 606

BLAST of CmoCh18G006350 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 595/606 (98.18%), Postives = 599/606 (98.84%), Query Frame = 0

Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRR SMSAANNNS MATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATC 60

Query: 61  IRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRD  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAE YADNVAFVEAVSGKAYTYREVVR
Sbjct: 61  IRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQ+VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLS L
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYG+IGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID+KGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAEAAS 607
           RAE AS
Sbjct: 601 RAETAS 606

BLAST of CmoCh18G006350 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.3e-297
Identity = 531/596 (89.09%), Postives = 560/596 (93.96%), Query Frame = 0

Query: 11  KKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEH 70
           +KR MPISP + L ISH QSM              PS     N SAM T IRDLVEDEEH
Sbjct: 25  QKRPMPISPPITLHISHSQSM-----------KTEPSSMPVANISAMGTLIRDLVEDEEH 84

Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKAYTYREVVRDT RFAKALS
Sbjct: 85  IFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTKRFAKALS 144

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
           SLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVE ADAKLVVT
Sbjct: 145 SLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEAADAKLVVT 204

Query: 191 NSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSS 250
           NS +FEKVKELKLPVIV+GEELIEGSMNWHKLLEAADRAGNN VKEDIKQ+DLCALPFSS
Sbjct: 205 NSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKEDIKQSDLCALPFSS 264

Query: 251 GTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNK 310
           GTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKVTTLGLIPFFHIYGITGICCATLRNK
Sbjct: 265 GTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHIYGITGICCATLRNK 324

Query: 311 GKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAA 370
           GKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAA
Sbjct: 325 GKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAA 384

Query: 371 PLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVK 430
           PLAPE+QT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+NL AKKNTVGRILPNLEVK
Sbjct: 385 PLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKNTVGRILPNLEVK 444

Query: 431 FIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDV 490
           FIDP+SGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID++GW+HTGDIGYIDD+GDV
Sbjct: 445 FIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWLHTGDIGYIDDNGDV 504

Query: 491 FIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAK 550
           FIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+V+APN+K
Sbjct: 505 FIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVIAPNSK 564

Query: 551 ETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 607
           ETE+EII+YVASNVA+YKKVR+VHFV++IPKSPSGKVMRRLIKEKMI+KIRA+ AS
Sbjct: 565 ETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKMIDKIRAKTAS 609

BLAST of CmoCh18G006350 vs. ExPASy TrEMBL
Match: A0A1S3BER3 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 5.3e-294
Identity = 519/592 (87.67%), Postives = 560/592 (94.59%), Query Frame = 0

Query: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRS 74
           MPISPS  LQ+SH QSMIQ FS   L RG+   + A N  SAM T  +D V+DE+HIFRS
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194
           KKG VV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYG+IGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDGDVFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+ ETE+
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 607
           EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 592

BLAST of CmoCh18G006350 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.8e-292
Identity = 515/554 (92.96%), Postives = 542/554 (97.83%), Query Frame = 0

Query: 53  NNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKA 112
           N SAM T IRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKA
Sbjct: 5   NISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKA 64

Query: 113 YTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 172
           YTYREVVRDT RFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH
Sbjct: 65  YTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 124

Query: 173 ISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNN 232
           ISEIKKQVE ADAKLVVTNS +FEKVKELKLPVIV+GEELIEGSMNWHKLLEAADRAGNN
Sbjct: 125 ISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNN 184

Query: 233 FVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIP 292
            VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKVTTLGLIP
Sbjct: 185 HVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIP 244

Query: 293 FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 352
           FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV
Sbjct: 245 FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 304

Query: 353 KEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDN 412
           +EFDLS LKLQAIMTAAAPLAPE+QT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+N
Sbjct: 305 EEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKEN 364

Query: 413 LAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDD 472
           L AKKNTVGRILPNLEVKFIDP+SGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID+
Sbjct: 365 LTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDN 424

Query: 473 KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD 532
           +GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD
Sbjct: 425 RGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD 484

Query: 533 KEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLI 592
           +EAGEIPAAS+V+APN+KETE+EII+YVASNVA+YKKVR+VHFV++IPKSPSGKVMRRLI
Sbjct: 485 EEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLI 544

Query: 593 KEKMIEKIRAEAAS 607
           KEKMI+KIRA+ AS
Sbjct: 545 KEKMIDKIRAKTAS 558

BLAST of CmoCh18G006350 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 787.3 bits (2032), Expect = 8.6e-228
Identity = 389/532 (73.12%), Postives = 459/532 (86.28%), Query Frame = 0

Query: 66  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRF 125
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 126 AKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 185
           AKAL+SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 186 KLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCA 245
           + ++T+++++EKVK L LPVIV+GEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 246 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 305
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 306 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAI 365
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 366 MTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILP 425
           MTAAAPLAPEL T+FE KFP V VQEAYGLTEHSCITL +G   K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 426 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYID 485
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID++GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 486 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM 545
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPD+EAGEIPAA +V+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 546 APNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMI 598
            P A E E++I+ +VA+NVAHYKKVR VHFV++IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of CmoCh18G006350 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 394.0 bits (1011), Expect = 2.1e-109
Identity = 203/551 (36.84%), Postives = 334/551 (60.62%), Query Frame = 0

Query: 52  NNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGK 111
           N+ +    C  D+      IFRS+LP++ +P+ + L +++ +N   +A     +   +G+
Sbjct: 9   NDQNDQKQCSNDV------IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGE 68

Query: 112 AYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAA 171
            YTY +V   + + A  L +L +K+  VV+++LPN  E  +  L     G + +  NP  
Sbjct: 69  VYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFF 128

Query: 172 HISEIKKQVEVADAKLVVTNSSSFEKVKELK----LPVIVVGEELIEGSMNWHKLLEAAD 231
             +EI KQ + + AKL+VT S   +K+K L+    L V    + + E  + + +L ++ +
Sbjct: 129 TPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEE 188

Query: 232 RAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKV 291
              ++ + E I   D+ ALPFSSGTTG+ KGVMLTH+ LV ++   + G    +      
Sbjct: 189 PRVDS-IPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDD 248

Query: 292 TTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILA 351
             L ++P FHIY +  I   +LR    +++M +F++   +  +   +VT A +VPPI+LA
Sbjct: 249 VILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLA 308

Query: 352 LVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNY 411
           + K+P  +++DLS +++  + + AAPL  EL+ +   KFP   + + YG+TE   +    
Sbjct: 309 IAKSPETEKYDLSSVRM--VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMS 368

Query: 412 GSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEE 471
               K+    K    G ++ N E+K +DPD+G SLP+N PGEIC+R   +M+GY N+   
Sbjct: 369 LGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLA 428

Query: 472 TSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDA 531
           T+ TID  GW+HTGD+G+IDDD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D 
Sbjct: 429 TASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDV 488

Query: 532 AVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSG 591
           AVV + +++AGE+P A +V + ++  +EDEI ++V+  V  YK++  V F ++IPK+PSG
Sbjct: 489 AVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSG 548

Query: 592 KVMRRLIKEKM 597
           K++R+ ++ ++
Sbjct: 549 KILRKDLRARL 550

BLAST of CmoCh18G006350 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 385.6 bits (989), Expect = 7.5e-107
Identity = 196/536 (36.57%), Postives = 322/536 (60.07%), Query Frame = 0

Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRS+LP++ +P+ ++L +++ QN   +A     +   +G  YTY +V   + + A    
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + ++ KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 191 NSSSFEKVKELK----LPVIVVGEE----LIEGSMNWHKLLEAADRAGNNFVKEDIKQTD 250
            +   +K+K L+    + ++ + +     + EG + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 251 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKVTTLGLIPFFHIYGIT 310
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 311 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 370
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   +++DLS +
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 371 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 430
           ++  + + AAPL  EL+ +   KFP   + + YG+TE   +        K+    K    
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 431 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 490
           G ++ N E+K +DPD+G SL +N PGEIC+R   +M+GY NN   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 491 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 550
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + ++ AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 551 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
           A +V + +++ +ED++ ++V+  V  YK++  V F E+IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of CmoCh18G006350 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 383.6 bits (984), Expect = 2.8e-106
Identity = 206/531 (38.79%), Postives = 322/531 (60.64%), Query Frame = 0

Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRS+LP++ +P+ + L  +  +   S +D    +   +GK+YTY E      R A  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
            L ++KG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 191 NSSSFEKVKEL--KLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKE-DIKQTDLCALP 250
           +S   +K+K L   L +I   E   E  + +  L+   D   N F +  DI   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 251 FSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKV--TTLGLIPFFHIYGITGICCA 310
           FSSGTTG+ KGV+LTH++L+ ++   + G    +  K     L ++P FHIY +  +   
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 311 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAI 370
           +LR+   V++M +F++   ++ +    VT A +VPP+++AL KNP V  +DLS ++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 371 MTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILP 430
           ++ AAPL  ELQ S  ++ P   + + YG+TE   +        K+ +  K  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 431 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYID 490
           N E+K +  ++  SL  N PGEIC+R Q +M+ Y N+ E TS TID++GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 491 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM 550
           +D ++FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  D+ AGE+P A +V 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 551 APNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
           +     TE+++ +YVA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CmoCh18G006350 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 376.7 bits (966), Expect = 3.5e-104
Identity = 197/554 (35.56%), Postives = 331/554 (59.75%), Query Frame = 0

Query: 66  EDEEH------IFRSQLPEVQVPDDITLPEFVLQ----NAESYADNVAFVEAVSGKAYTY 125
           EDEE       IFRS+LP++ +P+ + L ++V Q    + +  +     ++  +G+  TY
Sbjct: 17  EDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTY 76

Query: 126 REVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISE 185
            +V  +  R A  +  L ++ G VV+++LPN  E+A+  L +   G V +  NP     E
Sbjct: 77  ADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPE 136

Query: 186 IKKQVEVADAKLVVTNSSSFEKVKELKLPVIVV------GEELI-----EGSMNWHKLLE 245
           I KQ + + AK+++T     +K+  LK   +++      G+  +     +G +++ +L +
Sbjct: 137 IAKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQ 196

Query: 246 AADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--V 305
           A +      +K  I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    +   
Sbjct: 197 ADE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFT 256

Query: 306 GKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPI 365
                L  +P FHIY +  +  + +R    ++++ RF+L   +  +   +VT  P+ PP+
Sbjct: 257 ANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPV 316

Query: 366 ILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCIT 425
           +LA +K+P  + +DLS +++  +++ AA L  EL+ +   KFP     + YG+TE   + 
Sbjct: 317 VLAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVA 376

Query: 426 LNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNN 485
            +  +  K+    K    G ++ N E+K +D ++G SLP+N  GEICVR   +M+GY N+
Sbjct: 377 KSL-AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLND 436

Query: 486 EEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSI 545
            E T+RTID  GW+HTGDIG++DDD ++FIVDR+KELIK+KG+QVAPAELEA+L++HPSI
Sbjct: 437 PEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSI 496

Query: 546 EDAAVVPLPDKEAGEIPAASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKS 597
           +DAAVV + D+ A E+P A +  +  ++ TED++  YV   V HYK++++V F+E IPK+
Sbjct: 497 DDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKA 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQ121.2e-22673.124-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=... [more]
Q7XXL25.1e-20164.214-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9... [more]
P149122.6e-11238.954-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
P316842.6e-11238.724-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
A0A2H5AIX54.4e-11239.934-coumarate-CoA ligase 1 OS=Narcissus pseudonarcissus OX=39639 GN=4CL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G0B40.0e+00100.004-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=... [more]
A0A6J1HUV50.0e+0098.184-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ... [more]
A0A6J1DG052.3e-29789.094-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A1S3BER35.3e-29487.674-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1[more]
A0A6J1DEE13.8e-29292.964-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G62940.18.6e-22873.12acyl-CoA synthetase 5 [more]
AT3G21240.12.1e-10936.844-coumarate:CoA ligase 2 [more]
AT1G51680.17.5e-10736.574-coumarate:CoA ligase 1 [more]
AT1G65060.12.8e-10638.794-coumarate:CoA ligase 3 [more]
AT3G21230.13.5e-10435.564-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 93..502
e-value: 3.2E-96
score: 322.5
NoneNo IPR availableGENE3D3.30.300.30coord: 498..600
e-value: 1.5E-30
score: 107.9
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 63..599
NoneNo IPR availablePANTHERPTHR24096:SF3894-COUMARATE--COA LIGASE-LIKE 1coord: 63..599
NoneNo IPR availableCDDcd059044CLcoord: 81..590
e-value: 0.0
score: 667.016
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 72..599
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 511..586
e-value: 3.0E-16
score: 60.2
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 69..497
e-value: 7.7E-131
score: 438.7
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 246..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh18G006350.1CmoCh18G006350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046949 fatty-acyl-CoA biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity