CmoCh16G013100 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G013100
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Protease inhibitor protein
Location	Cmo_Chr16: 9304518 .. 9305069 (-)
RNA-Seq Expression	CmoCh16G013100
Synteny	CmoCh16G013100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTTGGCTATAAGTACGACACTCATTTGGCCGAACAAATTCAACAAAAGAGTACGCTAAGCATTTCCATATAACGAAGCAAAATGTCTGGTACGTGTCCTCTCCATATGTTCTTCTTATCATTTACTCTTAAAATTGTTTTGAAATTGTTTAGAAATGCTACGATGGAGCGGTTTGTAAGTCTTGGTATGGAATTATGTGCATTTTATTGTGAGTGATCTGTATTAACAAATTTAACACGAATATGTGTTTTAAATAGGGAAAAGTGAATGGCCTGAGCTTGTGTTCGTAGAATATTATGAAGTTGCAGATATAATACAAAAGGAGAATCCTAACGTCAAACCACAAAAGATTCTTGCCGGGAGTCCGATGATTTTGAATTACGATCCTTCACGAGTTTGGGTTATTGTCAATATTAAGGATGTTTGTATTTCACCTCCCAAAGTTGGTTAAAAATTACTCACGGCTATCGTTGTAAAAGTTATATGTACGTGTCTATATGAAAGTACTAATAAGGTAGTATCTAACTACCATTTTAATTATTGAATTGTTC

mRNA sequence

TGTTGGCTATAAGTACGACACTCATTTGGCCGAACAAATTCAACAAAAGAGTACGCTAAGCATTTCCATATAACGAAGCAAAATGTCTGGGAAAAGTGAATGGCCTGAGCTTGTGTTCGTAGAATATTATGAAGTTGCAGATATAATACAAAAGGAGAATCCTAACGTCAAACCACAAAAGATTCTTGCCGGGAGTCCGATGATTTTGAATTACGATCCTTCACGAGTTTGGGTTATTGTCAATATTAAGGATGTTTGTATTTCACCTCCCAAAGTTGGTTAAAAATTACTCACGGCTATCGTTGTAAAAGTTATATGTACGTGTCTATATGAAAGTACTAATAAGGTAGTATCTAACTACCATTTTAATTATTGAATTGTTC

Coding sequence (CDS)

ATGTCTGGGAAAAGTGAATGGCCTGAGCTTGTGTTCGTAGAATATTATGAAGTTGCAGATATAATACAAAAGGAGAATCCTAACGTCAAACCACAAAAGATTCTTGCCGGGAGTCCGATGATTTTGAATTACGATCCTTCACGAGTTTGGGTTATTGTCAATATTAAGGATGTTTGTATTTCACCTCCCAAAGTTGGTTAA

Protein sequence

MSGKSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISPPKVG

Homology

[Close]

BLAST of CmoCh16G013100 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.8e-06
Identity = 25/64 (39.06%), Postives = 40/64 (62.50%), Query Frame = 0


Query: 3  GKSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISP 62
          GKS WP LV V       II+++NPNVK   +  G+P+  ++  +RV + VN + + +SP
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 63 PKVG 67
          P++G
Sbjct: 65 PRIG 68

[Close]

BLAST of CmoCh16G013100 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 8.8e-06
Identity = 26/63 (41.27%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 3  GKSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISP 62
          GK EWPELV    Y+ A II++ENPNV+       S    ++   RVWV+V+   V +  
Sbjct: 6  GKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVVRT 65

Query: 63 PKV 66
          P+V
Sbjct: 66 PRV 68

[Close]

BLAST of CmoCh16G013100 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 2.2e-04
Identity = 25/63 (39.68%), Postives = 33/63 (52.38%), Query Frame = 0


Query: 3  GKSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISP 62
          GK+ WPELV       A  +++EN NV    +  GS M  ++   RVWVIVN   V  S 
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 63 PKV 66
          P +
Sbjct: 66 PHI 68

[Close]

BLAST of CmoCh16G013100 vs. ExPASy Swiss-Prot
Match: P16062 (Subtilisin-chymotrypsin inhibitor CI-1A OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 6.3e-04
Identity = 19/54 (35.19%), Postives = 35/54 (64.81%), Query Frame = 0


Query: 2  SGKSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNI 56
          S K+ WPE+V +   +  +II ++ PN + + I   + + LN+DP+RV+V+V +
Sbjct: 23 SAKTSWPEVVGMSAEKAKEIILRDKPNAQVEVIPVDAMVHLNFDPNRVFVLVAV 76

[Close]

BLAST of CmoCh16G013100 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 43.9 bits (102), Expect = 8.2e-04
Identity = 21/64 (32.81%), Postives = 33/64 (51.56%), Query Frame = 0


Query: 3  GKSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISP 62
          GK  WP+LV         +I++ENP V+   +  GSP+  ++   RV V V  + +   P
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 63 PKVG 67
          P +G
Sbjct: 65 PAIG 68

[Close]

BLAST of CmoCh16G013100 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.6e-13
Identity = 37/63 (58.73%), Postives = 51/63 (80.95%), Query Frame = 0


Query: 4  KSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISPP 63
          K+EWPELVFV+Y   A II+KENP+V+  KIL+G+P ILN+D +RVWV +N+++V +  P
Sbjct: 9  KTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEVVVKKP 68

Query: 64 KVG 67
          KVG
Sbjct: 69 KVG 71

[Close]

BLAST of CmoCh16G013100 vs. ExPASy TrEMBL
Match: A0A0A0L9Z4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142410 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.5e-13
Identity = 39/63 (61.90%), Postives = 47/63 (74.60%), Query Frame = 0


Query: 4  KSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISPP 63
          KS WPELV V++   A II+ ENPNVK  KIL GSP+ILNYDP+RVWVI N +D  +  P
Sbjct: 9  KSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNTEDKVVDIP 68

Query: 64 KVG 67
          +VG
Sbjct: 69 RVG 71

[Close]

BLAST of CmoCh16G013100 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.7e-12
Identity = 38/63 (60.32%), Postives = 46/63 (73.02%), Query Frame = 0


Query: 4  KSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISPP 63
          K+ WPELVFVE+   A II+KENP+VK  KILA SP I N+DPSRVWV  N++D  +  P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 64 KVG 67
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of CmoCh16G013100 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.1e-11
Identity = 34/62 (54.84%), Postives = 48/62 (77.42%), Query Frame = 0


Query: 4  KSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISPP 63
          K++WPELVF++Y   A II++ENP+V+  KIL+G+P ILN+D  RVW  VN++DV +  P
Sbjct: 10 KTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVKMP 69

Query: 64 KV 66
          KV
Sbjct: 70 KV 71

[Close]

BLAST of CmoCh16G013100 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.4e-11
Identity = 36/63 (57.14%), Postives = 46/63 (73.02%), Query Frame = 0


Query: 4  KSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISPP 63
          K++WPELVFV+Y   A II+KENP+VK  KIL+G   I N+D  RVW  VN++DV +  P
Sbjct: 10 KTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVEDVVVKTP 69

Query: 64 KVG 67
          KVG
Sbjct: 70 KVG 72

[Close]

BLAST of CmoCh16G013100 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 45.1 bits (105), Expect = 2.6e-05
Identity = 23/64 (35.94%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 3  GKSEWPELVFVEYYEVADIIQKENPNVKPQKILAGSPMILNYDPSRVWVIVNIKDVCISP 62
          GKS WPEL+  +  +  ++I++ENP +K   IL G+ +   +  SRV+V VN   + +  
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 63 PKVG 67
          P +G
Sbjct: 96 PIIG 99

The following BLAST results are available for this feature:

BLAST of CmoCh16G013100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	1.8e-06	39.06	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P80211	8.8e-06	41.27	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P82381	2.2e-04	39.68	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P16062	6.3e-04	35.19	Subtilisin-chymotrypsin inhibitor CI-1A OS=Hordeum vulgare OX=4513 PE=1 SV=1	[more]
P24076	8.2e-04	32.81	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]

BLAST of CmoCh16G013100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8E6	2.6e-13	58.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A0A0L9Z4	4.5e-13	61.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142410 PE=3 SV=1	[more]
A0A5A7U4U0	1.7e-12	60.32	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U228	1.1e-11	54.84	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	1.4e-11	57.14	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of CmoCh16G013100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	2.6e-05	35.94	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 4..66 e-value: 1.0E-14 score: 54.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 2..66
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..66 e-value: 1.2E-17 score: 66.0
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 2..66
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 2..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh16G013100.1	CmoCh16G013100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity