CmoCh16G012680 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh16G012680
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	Cmo_Chr16: 9040802 .. 9042315 (-)
RNA-Seq Expression	CmoCh16G012680
Synteny	CmoCh16G012680

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGTTGTTTTTCCGAATTTTGATTCTGATTCCATTACATTTCATTAGATTCTCACTCGCTAACGAATTTCGGAGCAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATGTAATTCACTCAAACACGTCGCTAATTCACTCTGTTGTATCGAATGATCACACGATATTTAATTTACCGAAGAAACTAAAACTCAGTTTCGTTCTAATTTTCAGAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGCAAGAACATTACAGAACAATTTTATTTTCATCTCCTTTAATCACGAGGCCTTTTACCATTATTGACGAACCGATGATGCAGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGGTATGAATAATGATTAATTTCCCTTCGCTTATGCATTACTGGCGGTTTGATCATAGCGACAGTGATATTCACTTGCAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGTTCTTCAATACATCACATTCATTGGCATTTTAATTGAATGATTTCACGTCCGCGTTGGATTTTGACACAATCGAAACAGGAAATGAAGGCGAATGTTACGGTAAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGGTACGAACTACCGAACAATTAAGCTCCTGCATGGAAACTAATCGATCTAAAAAGCGTATACAGATAATTAATTCAATGAGAATGCAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

mRNA sequence

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTAAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Coding sequence (CDS)

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTAAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Protein sequence

MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLMMIETAKSTGVQGRIVNVTSNIHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP

Homology

[Close]

BLAST of CmoCh16G012680 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 125/331 (37.76%), Postives = 182/331 (54.98%), Query Frame = 0


Query: 2   LEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRI 61
           +E++  + G AG SGYGS STAE+V+ S     +TAI+TG TSGIG E ARVL  RG  +
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 62  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM 121
           II +RN KAA ++K  I+     + ++RI  + LDLSS+ SV +F+  F +LN+PLN+L+
Sbjct: 61  IIASRNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILI 120

Query: 122 --------------------------------------MIETAKSTGVQGRIVNVTSNIH 181
                                                 M  +A+ +G++GRIVN++S  H
Sbjct: 121 NNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAH 180

Query: 182 --SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 241
             ++  G +F+Y+    +     Y   +AY  SKLAN+LH+  L+ KLQE   N+T+N V
Sbjct: 181 TYTYTEGIMFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSV 240

Query: 242 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 292
           HPG++ TNL R        +    A    L K IP+GAAT+CYVA + ++++V G YFAD
Sbjct: 241 HPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFAD 300

[Close]

BLAST of CmoCh16G012680 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.1e-46
Identity = 125/331 (37.76%), Postives = 180/331 (54.38%), Query Frame = 0


Query: 2   LEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRI 61
           +E++  + G AG SGYGS STAE+V+ S     +TAI+TG TSGIG E ARVL  RG  +
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 62  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM 121
           II ARN KAA ++K  I+     + ++RI  + LDLSS+ SV +F+  F +LN+PLN+L+
Sbjct: 61  IIAARNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILI 120

Query: 122 --------------------------------------MIETAKSTGVQGRIVNVTSNIH 181
                                                 M  +A+ +G++GRIVN++S  H
Sbjct: 121 NNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAH 180

Query: 182 --SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 241
             ++  G +F+Y+    +     Y   +AY  SKLAN+LH+  L+ KLQE   N+T+N V
Sbjct: 181 TYTYTEGIMFDYINDPDR-----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSV 240

Query: 242 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 292
           HPG++ TNL R        +    A    L K IP+GAAT+CYVA + +++ V G YF D
Sbjct: 241 HPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTD 300

[Close]

BLAST of CmoCh16G012680 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.3e-42
Identity = 124/329 (37.69%), Postives = 178/329 (54.10%), Query Frame = 0


Query: 7   YLVGSAGASGYGSKSTAEEVSESCMNS-ITAIVTGATSGIGEETARVLAKRGVRIIIPAR 66
           +  GS GASG+ S+STAEEV+     + +TAIVTGA+SGIG ETARVL+ RGV +++  R
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVR 61

Query: 67  NLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL------ 126
           N  +  + K  IV +      +++ VM LDLSS+ SV  F S ++S  LPLNLL      
Sbjct: 62  NTDSGAKVKEDIVKQ---VPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGI 121

Query: 127 ----------------------------MMIETAKSTG----VQGRIVNVTSNIH--SWF 186
                                       ++++T KST      +GRIVN++S  H  S+ 
Sbjct: 122 MACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYP 181

Query: 187 SGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVV 246
            G  F+   +I+ K+S  Y S RAY  SKL NVLH  +L  +L+E   N+T N +HPG +
Sbjct: 182 EGVRFD---KINDKSS--YSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAI 241

Query: 247 KTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQG 295
            TNL R    ++   +  +A    +LK++P+GAAT+CYVA N  V  V+G YF D N   
Sbjct: 242 MTNLGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAK 301

[Close]

BLAST of CmoCh16G012680 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.9e-36
Identity = 114/315 (36.19%), Postives = 163/315 (51.75%), Query Frame = 0


Query: 13  GASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAE 72
           G SG+   STAE+V+       +TAIVTGA+SGIG ET RVLA RG  +I+  RN+ AA+
Sbjct: 9   GVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAAK 68

Query: 73  EAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM----------- 132
           + K  I+ +      +++  + LDLSS++SV  F S F S   PLN+L+           
Sbjct: 69  DVKDTILKD---IPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFK 128

Query: 133 ---------------------------MIETAKSTGVQGRIVNVTSNIHSWFSGDIFEYL 192
                                      M +T + +  +GRIVNV S  H +   +   + 
Sbjct: 129 LSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF- 188

Query: 193 GQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRER 252
            +I+ ++S  Y++ RAY  SKLANVLH  QL   L+E   N+T N +HPG + TNL R  
Sbjct: 189 DKINDQSS--YNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRHN 248

Query: 253 EGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKS 289
              V  LI  +     +LK + +GAAT+CYVA +  V+ V+G YF+D N   +     K 
Sbjct: 249 SA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTP-HGKD 308

[Close]

BLAST of CmoCh16G012680 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.3e-21
Identity = 98/313 (31.31%), Postives = 145/313 (46.33%), Query Frame = 0


Query: 31  MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSE----SDCFDH 90
           M+  T ++TGA SG+G  TA  L + G R+I+  R+   AEEA  ++  E    ++C   
Sbjct: 41  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPE 100

Query: 91  ------SRITVMALDLSSVNSV-------------------------------------- 150
                   + V  LDL+S+ SV                                      
Sbjct: 101 PGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQ 160

Query: 151 --VNFVSNFESLNLPLNLLMMIETAKSTGVQGRIVNVTSNIHSWFSGDI-FEYLGQISQK 210
             VN + +F   NL L LL        +    RIV V+S ++ +  GDI F+ L      
Sbjct: 161 FGVNHLGHFLLTNLLLGLL-------KSSAPSRIVVVSSKLYKY--GDINFDDL-----N 220

Query: 211 NSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRE-REGFVT 270
           + + Y+ +  Y+ SKLAN+L T +LA +L+    NVTVN +HPG+V+TNL R      + 
Sbjct: 221 SEQSYNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLV 280

Query: 271 DLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAE 292
             +F + S +   KT  EGA TS Y+A++  VE V+G YF DC ++     K     +A 
Sbjct: 281 KPLFNLVSWA-FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLP-KAMDESVAR 335

[Close]

BLAST of CmoCh16G012680 vs. ExPASy TrEMBL
Match: A0A6J1EB90 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.8e-154
Identity = 296/335 (88.36%), Postives = 297/335 (88.66%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL+
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 --------------------------------------MIETAKSTGVQGRIVNVTSNIH 180
                                                 MIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 298
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

[Close]

BLAST of CmoCh16G012680 vs. ExPASy TrEMBL
Match: A0A6J1E6B3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.0e-152
Identity = 295/335 (88.06%), Postives = 296/335 (88.36%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL+
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 --------------------------------------MIETAKSTGVQGRIVNVTSNIH 180
                                                 MIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 298
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

[Close]

BLAST of CmoCh16G012680 vs. ExPASy TrEMBL
Match: A0A6J1HN85 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.2e-151
Identity = 291/335 (86.87%), Postives = 293/335 (87.46%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLL+
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 --------------------------------------MIETAKSTGVQGRIVNVTSNIH 180
                                                 MIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 298
           GVVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCN 300

[Close]

BLAST of CmoCh16G012680 vs. ExPASy TrEMBL
Match: A0A6J1HL83 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 3.9e-149
Identity = 290/335 (86.57%), Postives = 292/335 (87.16%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLL+
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 --------------------------------------MIETAKSTGVQGRIVNVTSNIH 180
                                                 MIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 298
           GVVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCN 300

[Close]

BLAST of CmoCh16G012680 vs. ExPASy TrEMBL
Match: A0A5A7UCR1 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00880 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 6.5e-128
Identity = 251/341 (73.61%), Postives = 276/341 (80.94%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           MLEM KYLVGSAGASGYGS STAEEV++S   +NSITAIVTGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSE +CFD+SRITVM LDLSS+NSV+NFVS FE+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LM--------------------------------------MIETAKSTGVQGRIVNVTSN 180
           L+                                      MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISQ----KNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVT 240
           IHSWFSGDIFEYL QIS+     NSR YD+TRAY+FSKLAN+LHTVQLAH+LQEMKANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGN 298
           VNCVHPGVV+TNLNR+REGF+ DLIF+MASKS+LLKTIP+ AATSCYVAT+R VENVNG 
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

[Close]

BLAST of CmoCh16G012680 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 6.3e-91
Identity = 189/328 (57.62%), Postives = 234/328 (71.34%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  KYL+G+AGASG+GSKSTAEEV+E+C + SITA++TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRSITAVITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL 120
           +I PARN+KAAEEAK RIVSE   F  + I VM LDLSS+ SV NFV++FESL+LPLNLL
Sbjct: 61  LIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120

Query: 121 M--------------------------------------MIETAKSTGVQGRIVNVTSNI 180
           +                                      MI+TA+ TGVQGRIVNVTS I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180

Query: 181 HSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           H WFSGD+ EYL  ISQ   + +D+TRAYA SKLANVLHT +L+ +LQ++ ANVTVNCVH
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQ-FDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 290
           PGVV+T L R+REG +TDL+F++ASK  L+KT+P+ AAT+CYVATN  + NV+G YF DC
Sbjct: 241 PGVVRTRLTRDREGLLTDLVFFLASK--LVKTVPQAAATTCYVATNPRLVNVSGKYFTDC 300

[Close]

BLAST of CmoCh16G012680 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 316.2 bits (809), Expect = 2.7e-86
Identity = 181/333 (54.35%), Postives = 228/333 (68.47%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVS-ESCMNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  K+L+GS G SG+GS+STA+ V+  S + S+TAI+TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRSLTAIITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL 120
           +++PAR++K AEE KARI+SE   F  + I VM LDLSS+ SV  FV +FESLNLPLN+L
Sbjct: 61  LVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120

Query: 121 M--------------------------------------MIETAKSTGVQGRIVNVTSNI 180
           +                                      MIETA  TGVQGRIVNVTS +
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180

Query: 181 HSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           HSWFSGD+ +YL  IS +N+R YD+TRAYA SKLANVLHTV+L+  L +M ANVT NCVH
Sbjct: 181 HSWFSGDMLQYLADIS-RNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 290
           PG+VKT L R+REG VTDL+F++ SK  LLK++P+ AAT+CYVAT+  + NV G YF+DC
Sbjct: 241 PGIVKTRLTRDREGVVTDLVFFLTSK--LLKSVPQAAATTCYVATSPRLRNVCGKYFSDC 300

[Close]

BLAST of CmoCh16G012680 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 220.7 bits (561), Expect = 1.6e-57
Identity = 134/324 (41.36%), Postives = 196/324 (60.49%), Query Frame = 0


Query: 6   KYLVGSAGASGYGSKSTAEEVSESCM---NSITAIVTGATSGIGEETARVLAKRGVRIII 65
           +YL G AG +G+GS+STAE+V++      + +TAI+TG TSGIG ETARVLAKRGVR+++
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVVM 65

Query: 66  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM-- 125
             R++K AE  K RI+ E+   D   I +  +DLSS++SV  F S F S +LPLN+L+  
Sbjct: 66  AVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNILINN 125

Query: 126 ------------------------------------MIETAKSTGVQGRIVNVTSNIHSW 185
                                               MI+TA+ +G++GRI+N++S IH+W
Sbjct: 126 AGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNW 185

Query: 186 FSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGV 245
              D F +  ++    SR Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHPG+
Sbjct: 186 VKPDCFSF-PKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 245

Query: 246 VKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQ 289
           VKT + R  +G  TD +F +ASK  LLK+I +GAAT+CYVA +   + ++G YFADCN+ 
Sbjct: 246 VKTGIIRAHKGLFTDSLFLIASK--LLKSISQGAATTCYVALSNETKGLSGKYFADCNET 305

[Close]

BLAST of CmoCh16G012680 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 198.4 bits (503), Expect = 8.3e-51
Identity = 128/332 (38.55%), Postives = 190/332 (57.23%), Query Frame = 0


Query: 4   MFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIII 63
           ++  + G  G SG+GS STAEEV++     ++TAI+TG T GIG ETARVL+KRG  ++I
Sbjct: 3   LYSLITGRRGPSGFGSASTAEEVTQGIDATNLTAIITGGTGGIGMETARVLSKRGAHVVI 62

Query: 64  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM-- 123
            ARN+ AAE AK  I+ ++    ++R+T++ LDLSS+ S+  FV  F +L+LPLNLL+  
Sbjct: 63  GARNMGAAENAKTEILRQN---ANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINN 122

Query: 124 ------------------------------------MIETAKSTGVQGRIVNVTS--NIH 183
                                               M  TAK++GV+GRI+NV+S  +I+
Sbjct: 123 AGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIY 182

Query: 184 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 243
           ++  G  F+ +  I       Y   RAY  SKLAN+LH  +L+ +LQE   N+T N VHP
Sbjct: 183 TYQEGIQFDSINDICS-----YSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHP 242

Query: 244 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 295
           G++ TNL +     +  L F+      L K IP+GAAT+CYVA + +V+ V G YFADCN
Sbjct: 243 GLILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCN 302

[Close]

BLAST of CmoCh16G012680 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 5.6e-47
Identity = 123/328 (37.50%), Postives = 184/328 (56.10%), Query Frame = 0


Query: 4   MFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIII 63
           ++  + G  G SG+GS STAE+V+++   + +TAI+TG TSGIG E ARVLA RG  +II
Sbjct: 3   IYGVMTGKKGKSGFGSASTAEDVTQAIDASHLTAIITGGTSGIGLEAARVLAMRGAHVII 62

Query: 64  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLM-- 123
            ARN KAA E+K  I+  +    ++R+  + +D+SS+ SV +FV  F +LN+PLN+L+  
Sbjct: 63  AARNPKAANESKEMILQMN---PNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILINN 122

Query: 124 ------------------------------------MIETAKSTGVQGRIVNVTSNIHSW 183
                                               M  TA+ +GVQGRIVN++S  H++
Sbjct: 123 AGVMFCPFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHTY 182

Query: 184 FSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGV 243
              +  ++ G     +   Y   RAY  SKL+N+LH+  L+ +LQE   N+T+N VHPG+
Sbjct: 183 TYSEGIKFQG---INDPAGYSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPGL 242

Query: 244 VKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQ 293
           V TNL R   GF   +  + A      K IP+GAAT+CYVA + ++E V G YF DCN  
Sbjct: 243 VTTNLFR-YSGF--SMKVFRAMTFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCNIV 302

The following BLAST results are available for this feature:

BLAST of CmoCh16G012680 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	1.2e-46	37.76	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	2.1e-46	37.76	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	5.3e-42	37.69	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	1.9e-36	36.19	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	1.3e-21	31.31	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of CmoCh16G012680 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EB90	1.8e-154	88.36	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]
A0A6J1E6B3	1.0e-152	88.06	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos...	[more]
A0A6J1HN85	7.2e-151	86.87	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]
A0A6J1HL83	3.9e-149	86.57	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max...	[more]
A0A5A7UCR1	6.5e-128	73.61	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of CmoCh16G012680 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	6.3e-91	57.62	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	2.7e-86	54.35	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	1.6e-57	41.36	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	8.3e-51	38.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G37540.1	5.6e-47	37.50	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 170..189 score: 30.23 coord: 35..52 score: 46.16 coord: 126..142 score: 27.08 coord: 193..210 score: 36.77
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 35..119 e-value: 2.4E-10 score: 40.2
None	No IPR available	GENE3D	3.40.50.720		coord: 15..118 e-value: 4.8E-18 score: 67.2
None	No IPR available	GENE3D	3.40.50.720		coord: 119..291 e-value: 1.6E-27 score: 98.3
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..120 coord: 121..291
None	No IPR available	PANTHER	PTHR24320:SF114	OS06G0129100 PROTEIN	coord: 4..120
None	No IPR available	PANTHER	PTHR24320:SF114	OS06G0129100 PROTEIN	coord: 121..291
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 35..259

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh16G012680.1	CmoCh16G012680.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity