CmoCh16G008210 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G008210
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.3-like
LocationCmo_Chr16: 4292574 .. 4296098 (-)
RNA-Seq ExpressionCmoCh16G008210
SyntenyCmoCh16G008210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATGGAAAGAAGGAGAGATTTCAAGGGGGAGAACAACGCCAACGCCAACGCCAACGCCATGCCATCTGAAGATGTCACCGTTGATTGGAGAGGACGACCCTCGAACGCCGTCTCCCACGGCGGAATGAGAGCAGCTGCTTTTGTTCTTGGTAACTCTCTTTCTCTTTTTATTCTAAAAAGAATGAGGACATGAATGAATCTGACAGAGTGAGCGTGTCGTGTGTGGTCGGTGAGCAGGACTGCAAGCGTTCGAGATAATGGCGATCGCTGCAGTTGGGAACAATCTGATAACATATCTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACATAGTGACAAACTTTGTTGGAACAGTGTTTCTTTTGGCACTTGTTGGTGGCTTCCTGTCTGATTCTTATCTTGGGAGTTTTTGGACCATGCTTATCTTTGGCTTTGTTGAACTTTCTGTAAGTTCCTCCTTTCATCATCATCGTTCTTCTTTCATTTCTATCAAAATCATTCTTTTAACACTCAAGATCACACATCAAACTCTCCATCCTTTCATGTTTATCAAAAATTTATATATGGTTTTCTCTTTTAGAACATATAATCAACAAGTTCGATGAATCAATCCCCTCAAACAAAGCTTATATCAAACTTAAACAGCATCTTAGTGCAATTATATTATAATGGACCAATTCGGACTTTCGCTCAACGTTTTTAAAACGTGTCTGCTAGAGAGAGGTTTCCACTCTCTTATGAAAAATGTTTTGTTACTCTCTCCAACCGACGTGAAATCTCACAATCCACCCCCTTCAAGGTCTAGTGTCTTTGTTGATACTTATTCCCTTTCTCCAATCGATGTGGGACCCCCTAATCCACTCTCTTCAGGGCCCAACGTCCTTGTTGGTACACAACCTCGTGTCTACCCCAAATCCACTGCTAGCAGATATTGTCCTCTTTAAACTTTTCCTTTCAGGTTTCCTCTCAAGATTTTTAAAACGCGTCAGTTTGAGAGAGATTTGCACACCTTTATAAAGAAATGTTTCGCTCCCCCTCTCCAACCGATATGGGATCTCACATGAAATCTTTCGGTTATAAGTTATACTTGTGAGCGCACATACACGTACATAATCTCAAGATAAGTCAAAGATTTTGCATGTGCATGCATATACGACAGATTATAGGGATAGTGAGAGAGAGGAGGGAGTAGTAAAGGGAAAGAAAGCCAAATAATATGCTGGTACTCATATGTGCAGGGATTCATATTGCTGTCGGTTCAGGCTCATGTTCCTCAGCTCAAGCCACCCCAATGCAACATGTTAGAAAGTGATGGAAATGAGTGTGTGGAAGCAAAAGGGTTAAAGGCATTGATATTCTTTGTGGCACTGTACTTAGTGGCTCTGGGGAGTGGGTGTGTGAAGCCCAATATGATAGCTCATGGGGCTGATCAGTTCAGTCCACAAAGTCCAACCCAATCCAAAACGCTCTCAACATACTTCAATGCAGCTTATTTGGCCTTCTCCATTGGAGAACTCATTGCGCTCACTCTTCTTGTTTGGATCCAAACTCACTCTGGCATGGACGTTGGCTTTGGAGTCTCTGCTGCTGTTATGGCTTTGGGTTTGATCAGTTTGGTTGCTGGCATTTTTTACTACAGGAATAAACCCCCTCAAGGACCCATTTTCACCCCTATTCTTCAAGTAACTCACTTTCTTTTCCTTCTACTTTTACTTAAGTTTACTGAGACTTGTCATAAAATTTGTTTGTTTCTTCTTAAAGCTTTCCTTTGTGTCCGAAAAATGTTCCCAAAACCATCCATTTTCGCCCGTTCCCTTTTCGTTATCCTGTTCTTAAAACATTCTATTTATGTTTGAATACTTTTGATCCATTACATTGTTCGTCATTGTACTGTTAGTTAACTTTTCATAGCGGTTTCCATAAGAACTTTAGAATCATATACTCTTACAACCCATGCTATCTCTATTAACTATAATACGTAATCTAAAATCTTTTAAGGTCGTATACATACATATAGACTCGTAGTGGGTAGACTCTTCGAAAGTTTAAAAGTTAGTGGTGGTCTGGTGAGTCATATTGTGCAGAAAAAGTTGAATCAAACTCGGTCATACTAATCCAAAAAATTGGTGTAAAAACACACAATTCTTCAGGTCTTTGTTGCTGCCCTTTTGAAAAGAAAGCAAGTTTGTCCTTCCAACCCTCATATGCTTCATGGAAGCCAAACCAGTAATGATAAAGTTGGCTTATCCTCTCACTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGCAAGTGCACTTCAATTCTCCATTTTGAACTTGTTTTAGAACCTCTCCTAAACTTTCCTTAAACAAACTCAAATCTTTACATGACTTTGAAACAAATTTCAGGTTCTTGAACAAGGCTTGTATCAAAAACCAGGATGAAGCAGCAAAGGAAAGCCCATGGAGATTGTGCACTCCCAATCAAGTTGAACAAGTGAAGATATTAGTTTCAGTTGTCCCAATATTTGCCTGCACAATAGTTTTCAATACCATATTGGCTCAGCTCCAAACATTTTCAGTTCAACAAGGAAGTCTCATGAACACCCAACTTACCAAATCCTTCCACATTCCACCAGCTTCCCTCCAGTCTATTCCTTACATTATGCTCATTATCATCGTCCCTTTGTATGATACATTCTTTGTCCCATTTGCTAGAAAATTCACTGGCCATACCTCTGGAATATCTCCTTTAAAACGTATTGGTTTCGGTCTCTTTCTCTCTGCCTTTTCGATGGTTTCAGCAGCCATAATGGAGAAAAAGAGGAGAGATTCAGCTGTGAATTTGAACCAGACGATATCGATCTTCTGGATCACACCACAGTTCCTTATTTTTGGGTTATCAGAGATGTTTACAGCAGTTGGACTCATTGAGTTCTTTTACAACCAATCAATAAAAGGGATGCAAGCATTTTTAACAGCCATGACATATTGCTCATACTCATTTGGATTCTATTTGAGCTCACTGCTTGTGTCAATGGTGAATAAGATCACATCGAGCTCCTCCAATGGAGGTTGGCTCCATGACAATAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTAAATTTTCTTAACTACCTCTTCTGGTCAAAATGGTTCGACGAACATCCATCCGTCTCACAAAAACCACAACATAATGACAACCCCCACCATGGAGAAGACTACCACATTTATAATTTCAATTCTTCAAAGAACAATGTTGATGATTATGTTCCTTGATCAAGAGATTTTCAGGCTACTTTTTTTTCTTTGTTTTTTAGATCAATTAGCCGATATGACCTAGATCGGTTGGGTGGGTGAATTGATAGAGTTTGTGTGATAGATGTGTACCTAATTCAGTAGATAAGACAGTAAACAAATATTATAAGCATGTGTACCTGTAAATGTCCCATTGTCTACGTCATATTATCTTCAAATGAATGACAAAAGTTGCTTTCTCCTCC

mRNA sequence

ATGGAGATGGAAAGAAGGAGAGATTTCAAGGGGGAGAACAACGCCAACGCCAACGCCAACGCCATGCCATCTGAAGATGTCACCGTTGATTGGAGAGGACGACCCTCGAACGCCGTCTCCCACGGCGGAATGAGAGCAGCTGCTTTTGTTCTTGGACTGCAAGCGTTCGAGATAATGGCGATCGCTGCAGTTGGGAACAATCTGATAACATATCTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACATAGTGACAAACTTTGTTGGAACAGTGTTTCTTTTGGCACTTGTTGGTGGCTTCCTGTCTGATTCTTATCTTGGGAGTTTTTGGACCATGCTTATCTTTGGCTTTGTTGAACTTTCTGGATTCATATTGCTGTCGGTTCAGGCTCATGTTCCTCAGCTCAAGCCACCCCAATGCAACATGTTAGAAAGTGATGGAAATGAGTGTGTGGAAGCAAAAGGGTTAAAGGCATTGATATTCTTTGTGGCACTGTACTTAGTGGCTCTGGGGAGTGGGTGTGTGAAGCCCAATATGATAGCTCATGGGGCTGATCAGTTCAGTCCACAAAGTCCAACCCAATCCAAAACGCTCTCAACATACTTCAATGCAGCTTATTTGGCCTTCTCCATTGGAGAACTCATTGCGCTCACTCTTCTTGTTTGGATCCAAACTCACTCTGGCATGGACGTTGGCTTTGGAGTCTCTGCTGCTGTTATGGCTTTGGGTTTGATCAGTTTGGTTGCTGGCATTTTTTACTACAGGAATAAACCCCCTCAAGGACCCATTTTCACCCCTATTCTTCAAGTCTTTGTTGCTGCCCTTTTGAAAAGAAAGCAAGTTTGTCCTTCCAACCCTCATATGCTTCATGGAAGCCAAACCAGTAATGATAAAGTTGGCTTATCCTCTCACTCTGCCAACCTTATTCCCACTCAAAAGTTCTTGAACAAGGCTTGTATCAAAAACCAGGATGAAGCAGCAAAGGAAAGCCCATGGAGATTGTGCACTCCCAATCAAGTTGAACAAGTGAAGATATTAGTTTCAGTTGTCCCAATATTTGCCTGCACAATAGTTTTCAATACCATATTGGCTCAGCTCCAAACATTTTCAGTTCAACAAGGAAGTCTCATGAACACCCAACTTACCAAATCCTTCCACATTCCACCAGCTTCCCTCCAGTCTATTCCTTACATTATGCTCATTATCATCGTCCCTTTGTATGATACATTCTTTGTCCCATTTGCTAGAAAATTCACTGGCCATACCTCTGGAATATCTCCTTTAAAACGTATTGGTTTCGGTCTCTTTCTCTCTGCCTTTTCGATGGTTTCAGCAGCCATAATGGAGAAAAAGAGGAGAGATTCAGCTGTGAATTTGAACCAGACGATATCGATCTTCTGGATCACACCACAGTTCCTTATTTTTGGGTTATCAGAGATGTTTACAGCAGTTGGACTCATTGAGTTCTTTTACAACCAATCAATAAAAGGGATGCAAGCATTTTTAACAGCCATGACATATTGCTCATACTCATTTGGATTCTATTTGAGCTCACTGCTTGTGTCAATGGTGAATAAGATCACATCGAGCTCCTCCAATGGAGGTTGGCTCCATGACAATAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTAAATTTTCTTAACTACCTCTTCTGGTCAAAATGGTTCGACGAACATCCATCCGTCTCACAAAAACCACAACATAATGACAACCCCCACCATGGAGAAGACTACCACATTTATAATTTCAATTCTTCAAAGAACAATGTTGATGATTATGTTCCTTGATCAAGAGATTTTCAGGCTACTTTTTTTTCTTTGTTTTTTAGATCAATTAGCCGATATGACCTAGATCGGTTGGGTGGGTGAATTGATAGAGTTTGTGTGATAGATGTGTACCTAATTCAGTAGATAAGACAGTAAACAAATATTATAAGCATGTGTACCTGTAAATGTCCCATTGTCTACGTCATATTATCTTCAAATGAATGACAAAAGTTGCTTTCTCCTCC

Coding sequence (CDS)

ATGGAGATGGAAAGAAGGAGAGATTTCAAGGGGGAGAACAACGCCAACGCCAACGCCAACGCCATGCCATCTGAAGATGTCACCGTTGATTGGAGAGGACGACCCTCGAACGCCGTCTCCCACGGCGGAATGAGAGCAGCTGCTTTTGTTCTTGGACTGCAAGCGTTCGAGATAATGGCGATCGCTGCAGTTGGGAACAATCTGATAACATATCTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACATAGTGACAAACTTTGTTGGAACAGTGTTTCTTTTGGCACTTGTTGGTGGCTTCCTGTCTGATTCTTATCTTGGGAGTTTTTGGACCATGCTTATCTTTGGCTTTGTTGAACTTTCTGGATTCATATTGCTGTCGGTTCAGGCTCATGTTCCTCAGCTCAAGCCACCCCAATGCAACATGTTAGAAAGTGATGGAAATGAGTGTGTGGAAGCAAAAGGGTTAAAGGCATTGATATTCTTTGTGGCACTGTACTTAGTGGCTCTGGGGAGTGGGTGTGTGAAGCCCAATATGATAGCTCATGGGGCTGATCAGTTCAGTCCACAAAGTCCAACCCAATCCAAAACGCTCTCAACATACTTCAATGCAGCTTATTTGGCCTTCTCCATTGGAGAACTCATTGCGCTCACTCTTCTTGTTTGGATCCAAACTCACTCTGGCATGGACGTTGGCTTTGGAGTCTCTGCTGCTGTTATGGCTTTGGGTTTGATCAGTTTGGTTGCTGGCATTTTTTACTACAGGAATAAACCCCCTCAAGGACCCATTTTCACCCCTATTCTTCAAGTCTTTGTTGCTGCCCTTTTGAAAAGAAAGCAAGTTTGTCCTTCCAACCCTCATATGCTTCATGGAAGCCAAACCAGTAATGATAAAGTTGGCTTATCCTCTCACTCTGCCAACCTTATTCCCACTCAAAAGTTCTTGAACAAGGCTTGTATCAAAAACCAGGATGAAGCAGCAAAGGAAAGCCCATGGAGATTGTGCACTCCCAATCAAGTTGAACAAGTGAAGATATTAGTTTCAGTTGTCCCAATATTTGCCTGCACAATAGTTTTCAATACCATATTGGCTCAGCTCCAAACATTTTCAGTTCAACAAGGAAGTCTCATGAACACCCAACTTACCAAATCCTTCCACATTCCACCAGCTTCCCTCCAGTCTATTCCTTACATTATGCTCATTATCATCGTCCCTTTGTATGATACATTCTTTGTCCCATTTGCTAGAAAATTCACTGGCCATACCTCTGGAATATCTCCTTTAAAACGTATTGGTTTCGGTCTCTTTCTCTCTGCCTTTTCGATGGTTTCAGCAGCCATAATGGAGAAAAAGAGGAGAGATTCAGCTGTGAATTTGAACCAGACGATATCGATCTTCTGGATCACACCACAGTTCCTTATTTTTGGGTTATCAGAGATGTTTACAGCAGTTGGACTCATTGAGTTCTTTTACAACCAATCAATAAAAGGGATGCAAGCATTTTTAACAGCCATGACATATTGCTCATACTCATTTGGATTCTATTTGAGCTCACTGCTTGTGTCAATGGTGAATAAGATCACATCGAGCTCCTCCAATGGAGGTTGGCTCCATGACAATAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTAAATTTTCTTAACTACCTCTTCTGGTCAAAATGGTTCGACGAACATCCATCCGTCTCACAAAAACCACAACATAATGACAACCCCCACCATGGAGAAGACTACCACATTTATAATTTCAATTCTTCAAAGAACAATGTTGATGATTATGTTCCTTGA

Protein sequence

MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKVGLSSHSANLIPTQKFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHIYNFNSSKNNVDDYVP
Homology
BLAST of CmoCh16G008210 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 4.8e-231
Identity = 413/553 (74.68%), Postives = 469/553 (84.81%), Query Frame = 0

Query: 24  SEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKS 83
           +E+ +VDWRGRPSN   HGGMRAA FVLGLQAFEIM IAAVGNNLITY+INEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 84  ANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQC 143
           ANIVTNFVGT+F+ AL+GG+LSD++LGSFWT++IFGFVELSGFILLSVQAH+PQLKPP+C
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKC 144

Query: 144 NMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLST 203
           N L      C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQFS   P QSK LS+
Sbjct: 145 NPLID--QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSS 204

Query: 204 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 263
           YFNAAY AFS+GELIALTLLVW+QTHSGMD+GFGVSAA M +G+ISLV+G  Y+RNK P+
Sbjct: 205 YFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPR 264

Query: 264 GPIFTPILQVFVAALLKRKQVCPSNPHMLHGS-QTSNDKVGLSSHSANLIPTQKFLNKAC 323
             IFTPI  V VAA+LKRK   PS+P MLHG    +ND V   S +    P  +FL+KAC
Sbjct: 265 RSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVV--PSSTLPHTPRFRFLDKAC 324

Query: 324 IKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNT 383
           IK QD   KESPWRLCT  QVEQVK L+S+VPIFA TIVFNTILAQLQTFSVQQGS MNT
Sbjct: 325 IKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNT 384

Query: 384 QLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSA 443
           +L+ SFHIPPASLQ+IPYIMLI +VPLYD+F VPFARK TGH SGI PL RIG GLFLS 
Sbjct: 385 RLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLST 444

Query: 444 FSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGMQ 503
           FSMVSAA++EKKRRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFY QS KGM+
Sbjct: 445 FSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGME 504

Query: 504 AFLTAMTYCSYSFGFYLSSLLVSMVNKITSSS-SNGGWLHDNNLNKDRLDLFYWLLAALS 563
           +FL A+TYCSYSFGFY SS+LVS+VNKITS+S  + GWL +N+LNKDRLDLFYWLLA LS
Sbjct: 505 SFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLS 564

Query: 564 FLNFLNYLFWSKW 575
            LNFL+YLFWS+W
Sbjct: 565 LLNFLSYLFWSRW 573

BLAST of CmoCh16G008210 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 5.9e-221
Identity = 395/561 (70.41%), Postives = 465/561 (82.89%), Query Frame = 0

Query: 25  EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 84
           +D+ VD+RGRP     HGG RAA FVLG QAFE+MAIAAVGNNLITY+ NEMHF LSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 85  NIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 144
           N+VTNF+GTVFLL+L+GGFLSDSYLGSF TML+FG +E+SGFILLSVQAH+P+L+PP+CN
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 145 MLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTY 204
           M +S    CVEA G KA   + AL LVALGSGC+KPN+I+HGA+QF  +     + LS++
Sbjct: 143 M-KSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRK---DLRKLSSF 202

Query: 205 FNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQG 264
           FNAAY AFS+G+LIALTLLVW+QTHSGMDVGFGVSAAVMA G+ISLVAG  +YRNKPP G
Sbjct: 203 FNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSG 262

Query: 265 PIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKVGLSSHSANLIPTQKFLNKACIK 324
            IFTPI QVFVAA+ KRKQ+CPSNP+M+H   T   +V    HS       +FL+KACIK
Sbjct: 263 SIFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKF----RFLDKACIK 322

Query: 325 NQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNTQL 384
            Q + A ESPWRLCT  QV QVKIL+SV+PIFACTI+FNTILAQLQTFSVQQGS MNT +
Sbjct: 323 TQGK-AMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 382

Query: 385 TKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSAFS 444
           TK+F IPPASLQ+IPYI+LI  VPLY+TFFVP ARK TG+ SGISPL+RIG GLFL+ FS
Sbjct: 383 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 442

Query: 445 MVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGMQAF 504
           MV+AA++EKKRR+S +  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFY QS + MQ+F
Sbjct: 443 MVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSF 502

Query: 505 LTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNG---GWLHDNNLNKDRLDLFYWLLAALS 564
           LTAMTYCSYSFGFYLSS+LVS VN++TSS+ +G   GWL DN+LNKDRLD FYWLLA+LS
Sbjct: 503 LTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLS 562

Query: 565 FLNFLNYLFWSKWFDEHPSVS 583
           F+NF NYLFWS+W+   PS +
Sbjct: 563 FINFFNYLFWSRWYSCDPSAT 574

BLAST of CmoCh16G008210 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 4.5e-112
Identity = 238/566 (42.05%), Postives = 342/566 (60.42%), Query Frame = 0

Query: 30  DWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTN 89
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ YL   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 90  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESD 149
           F+GT FLLAL+GGFLSD++  +F   LI   +E  G I+L++QA  P L PP C     D
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-----D 134

Query: 150 GNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTYFNAAY 209
              C E  G KA + FV LYLVALG G +K ++ +HGA+QF   +P   K  ST+FN   
Sbjct: 135 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFV 194

Query: 210 LAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTP 269
              + G L+A+T +VW++ + G + GFGVS   + + ++  ++G  +YRNK P G   T 
Sbjct: 195 FCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTT 254

Query: 270 ILQVFVAALLK------------RKQVCPSNPHMLHGSQTSNDKVGLSS-HSANLIPTQK 329
           IL+V +AA +K               V PSN  +  G +    +  L        +P + 
Sbjct: 255 ILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRA 314

Query: 330 FLNKACIKNQDEAAKESP-WRL--CTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFS 389
            L  + +K  + AA E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL TFS
Sbjct: 315 QLTNS-LKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFS 374

Query: 390 VQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKR 449
           VQQ + MNT++  S  IPPASL   P + ++I+ P+YD   +PFARK T   +G++ L+R
Sbjct: 375 VQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQR 434

Query: 450 IGFGLFLSAFSMVSAAIMEKKRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTA 509
           IG GL LS  +M  AA++E KR+  A     ++  +T  ++  WI  Q+L  G +++FT 
Sbjct: 435 IGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTL 494

Query: 510 VGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLN 569
            GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N
Sbjct: 495 AGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSIN 554

Query: 570 KDRLDLFYWLLAALSFLNFLNYLFWS 573
           + +LD FYWL+  LS  NFL+YLFW+
Sbjct: 555 RYKLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of CmoCh16G008210 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 6.7e-108
Identity = 224/552 (40.58%), Postives = 330/552 (59.78%), Query Frame = 0

Query: 30  DWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTN 89
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ YL N MH SL++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 90  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESD 149
           F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA  P L PP C    S 
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK--SSA 136

Query: 150 GNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTYFNAAY 209
              C    G KA   FV LYLV+LG G +K ++ +HGA+QF   +P   K  ST+FN   
Sbjct: 137 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYV 196

Query: 210 LAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTP 269
              S G L+A+T +VWI+ + G + GFGVS   + L ++  + G  +Y+NK P+G   T 
Sbjct: 197 FCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTT 256

Query: 270 ILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKVGLSSHSANLIPTQKFLNK-ACIKNQDE 329
           I +V +AA +       S+ H      TS +   + S      P+Q   N   C+    E
Sbjct: 257 IFKVLLAASIVSCSSKTSSNHF-----TSRE---VQSEHEEKTPSQSLTNSLTCLNKAIE 316

Query: 330 AAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSF 389
                 W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  +F
Sbjct: 317 GKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NF 376

Query: 390 HIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSA 449
           ++P ASL   P + ++I+ P YD   +PFARK T    GI+ L+RIG GL LS  +M  A
Sbjct: 377 NVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVA 436

Query: 450 AIMEKKRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGM 509
           A++E KR+  A     ++  +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++   M
Sbjct: 437 ALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSM 496

Query: 510 QAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAALS 569
           ++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  LS
Sbjct: 497 RSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCVLS 556

Query: 570 FLNFLNYLFWSK 574
            +NFL+YLFW+K
Sbjct: 557 VVNFLHYLFWAK 556

BLAST of CmoCh16G008210 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 7.7e-104
Identity = 216/571 (37.83%), Postives = 341/571 (59.72%), Query Frame = 0

Query: 24  SEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKS 83
           ++D TVD    P+N    G  +A  F+LG +  E +A   +G NL+ YL + ++   + +
Sbjct: 8   TQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATA 67

Query: 84  ANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQC 143
           AN VTN+ GT ++  L+G F++D+YLG +WT+  F F+ +SG  LL++ A VP LKP  C
Sbjct: 68  ANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC 127

Query: 144 NMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLST 203
           N       +       +  +FFVALY++ALG+G +KP + + GADQF      +    S+
Sbjct: 128 NA------DTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSS 187

Query: 204 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 263
           +FN  Y + ++G LIA T+LVWIQ + G   GFGV    M + +     G  +YR + P 
Sbjct: 188 FFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPG 247

Query: 264 GPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKVGLSSHSANLIPTQ--KFLNKA 323
           G   T I QV VAA  K     P +  +L   +T++D+  +   S  L+ T   KF +KA
Sbjct: 248 GSPLTRIFQVIVAAFRKISVKVPEDKSLLF--ETADDESNIKG-SRKLVHTDNLKFFDKA 307

Query: 324 CIKNQDEAAKE---SPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGS 383
            +++Q ++ K+   +PWRLC+  QVE++K +++++P++A  IVF T+ +Q+ T  V QG+
Sbjct: 308 AVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGN 367

Query: 384 LMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGL 443
            M+  + K+F IP ASL     + ++   P+YD F +P ARKFT +  G + L+R+G GL
Sbjct: 368 TMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGL 427

Query: 444 FLSAFSMVSAAIMEKKRRDSAVNLNQ------TISIFWITPQFLIFGLSEMFTAVGLIEF 503
            +S F+M++A ++E  R D     N        +SIFW  PQ+L+ G +E+FT +G +EF
Sbjct: 428 VVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEF 487

Query: 504 FYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDL 563
           FY+Q+   M++  +A++  + + G YLS++LV++V KIT  +   GW+ D NLN+  LD 
Sbjct: 488 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDY 547

Query: 564 FYWLLAALSFLNFLNYLFWSKWFDEHPSVSQ 584
           F++LLA LSFLNFL YL+ SK +    +V +
Sbjct: 548 FFYLLATLSFLNFLVYLWISKRYKYKKAVGR 568

BLAST of CmoCh16G008210 vs. ExPASy TrEMBL
Match: A0A6J1EMI3 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435878 PE=3 SV=1)

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 614/616 (99.68%), Postives = 614/616 (99.68%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA
Sbjct: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
           IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240
           NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300
           AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 301 KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360
           KVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420
           TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480
           RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540
           GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600
           WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 601 IYNFNSSKNNVDDYVP 615
           IYNFNSSKNNVDDYVP
Sbjct: 601 IYNFNSSKNNVDDYVP 616

BLAST of CmoCh16G008210 vs. ExPASy TrEMBL
Match: A0A6J1JCZ7 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483371 PE=3 SV=1)

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 604/616 (98.05%), Postives = 608/616 (98.70%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERRRDFKGEN  NANANAMPSEDVTVDWRGRPSN+VSHGGMRAAAFVLGLQAFEIMA
Sbjct: 1   MEMERRRDFKGEN--NANANAMPSEDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMA 60

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
           IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAHVPQLKPPQCNML+SDGNECVEAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240
           NMIAHGADQFSPQSP+QSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300
           AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 301 KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360
           KVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420
           TIVFNTILAQLQTFSVQQGSLMNTQLTKS HIPPASLQSIPYIMLI+IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFA 420

Query: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480
           RK TGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540
           GLSEMFTAVGLIEFFY QSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600
           WLHDNNLNKDRLDLFYWLLAALSFLN LNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 601 IYNFNSSKNNVDDYVP 615
           IYNFNSSKNNVDDYVP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of CmoCh16G008210 vs. ExPASy TrEMBL
Match: A0A6J1ERH0 (protein NRT1/ PTR FAMILY 4.3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435878 PE=3 SV=1)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 572/616 (92.86%), Postives = 572/616 (92.86%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVL         
Sbjct: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVL--------- 60

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
                                            VFLLALVGGFLSDSYLGSFWTMLIFGF
Sbjct: 61  ---------------------------------VFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240
           NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300
           AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 301 KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360
           KVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420
           TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480
           RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540
           GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600
           WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 574

Query: 601 IYNFNSSKNNVDDYVP 615
           IYNFNSSKNNVDDYVP
Sbjct: 601 IYNFNSSKNNVDDYVP 574

BLAST of CmoCh16G008210 vs. ExPASy TrEMBL
Match: A0A6J1JB17 (protein NRT1/ PTR FAMILY 4.3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483371 PE=3 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 562/616 (91.23%), Postives = 566/616 (91.88%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERRRDFKGEN  NANANAMPSEDVTVDWRGRPSN+VSHGGMRAAAFVL         
Sbjct: 1   MEMERRRDFKGEN--NANANAMPSEDVTVDWRGRPSNSVSHGGMRAAAFVL--------- 60

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
                                            VFLLALVGGFLSDSYLGSFWTMLIFGF
Sbjct: 61  ---------------------------------VFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAHVPQLKPPQCNML+SDGNECVEAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240
           NMIAHGADQFSPQSP+QSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300
           AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 301 KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360
           KVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420
           TIVFNTILAQLQTFSVQQGSLMNTQLTKS HIPPASLQSIPYIMLI+IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFA 420

Query: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480
           RK TGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540
           GLSEMFTAVGLIEFFY QSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600
           WLHDNNLNKDRLDLFYWLLAALSFLN LNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 572

Query: 601 IYNFNSSKNNVDDYVP 615
           IYNFNSSKNNVDDYVP
Sbjct: 601 IYNFNSSKNNVDDYVP 572

BLAST of CmoCh16G008210 vs. ExPASy TrEMBL
Match: A0A6J1C7K1 (protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.1e-307
Identity = 543/618 (87.86%), Postives = 572/618 (92.56%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERR+DFKG+ +          + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMA
Sbjct: 3   MEMERRKDFKGDED---------RDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMA 62

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
           IAAVGNNLITY INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGF
Sbjct: 63  IAAVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGF 122

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAH+PQLKPPQCNML  DG  C EAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 123 VELSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKP 182

Query: 181 NMIAHGADQFSPQ-SPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVS 240
           NMIAHGADQFSPQ +P  SK LSTYFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVS
Sbjct: 183 NMIAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVS 242

Query: 241 AAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSN 300
           AAVMA+GLISLV GIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQ + 
Sbjct: 243 AAVMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNV 302

Query: 301 D-KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF 360
           D KV LSS SANLIPTQK  FL+KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIF
Sbjct: 303 DHKVALSSDSANLIPTQKFRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIF 362

Query: 361 ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP 420
           ACTI+FNTILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVP
Sbjct: 363 ACTIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVP 422

Query: 421 FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFL 480
           FARKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSA+NLNQT+SIFWITPQFL
Sbjct: 423 FARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFL 482

Query: 481 IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 540
           IFGLSEMFTAVGLIEFFY QS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSN
Sbjct: 483 IFGLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSN 542

Query: 541 GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED 600
           GGWLHDNNLNKDRLDLFYWLLA LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGED
Sbjct: 543 GGWLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGED 602

Query: 601 YHIYNFNSSKNNVDDYVP 615
           YH YNFNSSK N+D+YVP
Sbjct: 603 YHSYNFNSSKTNIDEYVP 611

BLAST of CmoCh16G008210 vs. NCBI nr
Match: XP_022929221.1 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 614/616 (99.68%), Postives = 614/616 (99.68%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA
Sbjct: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
           IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240
           NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300
           AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 301 KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360
           KVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420
           TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480
           RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540
           GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600
           WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 601 IYNFNSSKNNVDDYVP 615
           IYNFNSSKNNVDDYVP
Sbjct: 601 IYNFNSSKNNVDDYVP 616

BLAST of CmoCh16G008210 vs. NCBI nr
Match: KAG7015441.1 (Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 611/618 (98.87%), Postives = 611/618 (98.87%), Query Frame = 0

Query: 1   MEMERRRDFKGEN--NANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEI 60
           MEMERRRDFKGEN  NANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEI
Sbjct: 1   MEMERRRDFKGENNANANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEI 60

Query: 61  MAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIF 120
           MAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLAL GGFLSDSYLGSFWTMLIF
Sbjct: 61  MAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALFGGFLSDSYLGSFWTMLIF 120

Query: 121 GFVELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCV 180
           GFVELSGFILLSVQAHVPQLKPPQCNM ESDGNECVEAKGLKALIFFVALYLVALGSGCV
Sbjct: 121 GFVELSGFILLSVQAHVPQLKPPQCNMSESDGNECVEAKGLKALIFFVALYLVALGSGCV 180

Query: 181 KPNMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGV 240
           KPNMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGV
Sbjct: 181 KPNMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGV 240

Query: 241 SAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTS 300
           SAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTS
Sbjct: 241 SAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTS 300

Query: 301 NDKVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF 360
           NDKVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF
Sbjct: 301 NDKVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF 360

Query: 361 ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP 420
           ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP
Sbjct: 361 ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP 420

Query: 421 FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFL 480
           FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNL QTISIFWITPQFL
Sbjct: 421 FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLTQTISIFWITPQFL 480

Query: 481 IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 540
           IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN
Sbjct: 481 IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 540

Query: 541 GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED 600
           GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED
Sbjct: 541 GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED 600

Query: 601 YHIYNFNSSKNNVDDYVP 615
           YHIYNFNSSKNNVDDYVP
Sbjct: 601 YHIYNFNSSKNNVDDYVP 618

BLAST of CmoCh16G008210 vs. NCBI nr
Match: XP_023552004.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 609/616 (98.86%), Postives = 611/616 (99.19%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERRRDFKGEN  NANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA
Sbjct: 1   MEMERRRDFKGEN--NANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
           IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240
           NMIAHGADQFSPQSP+QSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300
           AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 301 KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360
           KVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420
           TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480
           RKFTGHTSGISPLKRIGFGLFLSAFSMV+AAIMEKKRRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVAAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540
           GLSEMFTAVGLIEFFY QSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600
           WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 601 IYNFNSSKNNVDDYVP 615
           IYNFNSSKNNVDDYVP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of CmoCh16G008210 vs. NCBI nr
Match: XP_022985329.1 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1179.9 bits (3051), Expect = 0.0e+00
Identity = 604/616 (98.05%), Postives = 608/616 (98.70%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60
           MEMERRRDFKGEN  NANANAMPSEDVTVDWRGRPSN+VSHGGMRAAAFVLGLQAFEIMA
Sbjct: 1   MEMERRRDFKGEN--NANANAMPSEDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMA 60

Query: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120
           IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180
           VELSGFILLSVQAHVPQLKPPQCNML+SDGNECVEAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240
           NMIAHGADQFSPQSP+QSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300
           AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 301 KVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360
           KVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420
           TIVFNTILAQLQTFSVQQGSLMNTQLTKS HIPPASLQSIPYIMLI+IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFA 420

Query: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480
           RK TGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF
Sbjct: 421 RKITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540
           GLSEMFTAVGLIEFFY QSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600
           WLHDNNLNKDRLDLFYWLLAALSFLN LNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 601 IYNFNSSKNNVDDYVP 615
           IYNFNSSKNNVDDYVP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of CmoCh16G008210 vs. NCBI nr
Match: KAG6577354.1 (Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 608/622 (97.75%), Postives = 608/622 (97.75%), Query Frame = 0

Query: 1   MEMERRRDFKGENNANA------NANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQ 60
           MEME RRDFKGENNANA      NANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQ
Sbjct: 1   MEME-RRDFKGENNANANTNANTNANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQ 60

Query: 61  AFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWT 120
           AFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLAL GGFLSDSYLGSFWT
Sbjct: 61  AFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALFGGFLSDSYLGSFWT 120

Query: 121 MLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALG 180
           MLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALG
Sbjct: 121 MLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALG 180

Query: 181 SGCVKPNMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDV 240
           SGCVKPNMIAHGADQFSPQSPTQSKT STYFNAAYLAFSIGELIALTLLVWIQTHSGMDV
Sbjct: 181 SGCVKPNMIAHGADQFSPQSPTQSKTHSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDV 240

Query: 241 GFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHG 300
           GFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHG
Sbjct: 241 GFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHG 300

Query: 301 SQTSNDKVGLSSHSANLIPTQK--FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSV 360
           SQTSNDKVGLSSHSANLIPTQK  FLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSV
Sbjct: 301 SQTSNDKVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSV 360

Query: 361 VPIFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDT 420
           VPIFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDT
Sbjct: 361 VPIFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDT 420

Query: 421 FFVPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWIT 480
            FVPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWIT
Sbjct: 421 LFVPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWIT 480

Query: 481 PQFLIFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITS 540
           PQFLIFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSM NKITS
Sbjct: 481 PQFLIFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMANKITS 540

Query: 541 SSSNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPH 600
           SSSNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWF EHPSVSQKPQHNDNPH
Sbjct: 541 SSSNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFAEHPSVSQKPQHNDNPH 600

Query: 601 HGEDYHIYNFNSSKNNVDDYVP 615
           HGEDYHIYNFNSSKNNVDDYVP
Sbjct: 601 HGEDYHIYNFNSSKNNVDDYVP 621

BLAST of CmoCh16G008210 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 802.0 bits (2070), Expect = 3.4e-232
Identity = 413/553 (74.68%), Postives = 469/553 (84.81%), Query Frame = 0

Query: 24  SEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKS 83
           +E+ +VDWRGRPSN   HGGMRAA FVLGLQAFEIM IAAVGNNLITY+INEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 84  ANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQC 143
           ANIVTNFVGT+F+ AL+GG+LSD++LGSFWT++IFGFVELSGFILLSVQAH+PQLKPP+C
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKC 144

Query: 144 NMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLST 203
           N L      C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQFS   P QSK LS+
Sbjct: 145 NPLID--QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSS 204

Query: 204 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 263
           YFNAAY AFS+GELIALTLLVW+QTHSGMD+GFGVSAA M +G+ISLV+G  Y+RNK P+
Sbjct: 205 YFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPR 264

Query: 264 GPIFTPILQVFVAALLKRKQVCPSNPHMLHGS-QTSNDKVGLSSHSANLIPTQKFLNKAC 323
             IFTPI  V VAA+LKRK   PS+P MLHG    +ND V   S +    P  +FL+KAC
Sbjct: 265 RSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVV--PSSTLPHTPRFRFLDKAC 324

Query: 324 IKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNT 383
           IK QD   KESPWRLCT  QVEQVK L+S+VPIFA TIVFNTILAQLQTFSVQQGS MNT
Sbjct: 325 IKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNT 384

Query: 384 QLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSA 443
           +L+ SFHIPPASLQ+IPYIMLI +VPLYD+F VPFARK TGH SGI PL RIG GLFLS 
Sbjct: 385 RLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLST 444

Query: 444 FSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGMQ 503
           FSMVSAA++EKKRRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFY QS KGM+
Sbjct: 445 FSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGME 504

Query: 504 AFLTAMTYCSYSFGFYLSSLLVSMVNKITSSS-SNGGWLHDNNLNKDRLDLFYWLLAALS 563
           +FL A+TYCSYSFGFY SS+LVS+VNKITS+S  + GWL +N+LNKDRLDLFYWLLA LS
Sbjct: 505 SFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLS 564

Query: 564 FLNFLNYLFWSKW 575
            LNFL+YLFWS+W
Sbjct: 565 LLNFLSYLFWSRW 573

BLAST of CmoCh16G008210 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 768.5 bits (1983), Expect = 4.2e-222
Identity = 395/561 (70.41%), Postives = 465/561 (82.89%), Query Frame = 0

Query: 25  EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 84
           +D+ VD+RGRP     HGG RAA FVLG QAFE+MAIAAVGNNLITY+ NEMHF LSKSA
Sbjct: 23  DDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSA 82

Query: 85  NIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 144
           N+VTNF+GTVFLL+L+GGFLSDSYLGSF TML+FG +E+SGFILLSVQAH+P+L+PP+CN
Sbjct: 83  NLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECN 142

Query: 145 MLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTY 204
           M +S    CVEA G KA   + AL LVALGSGC+KPN+I+HGA+QF  +     + LS++
Sbjct: 143 M-KSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRK---DLRKLSSF 202

Query: 205 FNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQG 264
           FNAAY AFS+G+LIALTLLVW+QTHSGMDVGFGVSAAVMA G+ISLVAG  +YRNKPP G
Sbjct: 203 FNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSG 262

Query: 265 PIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKVGLSSHSANLIPTQKFLNKACIK 324
            IFTPI QVFVAA+ KRKQ+CPSNP+M+H   T   +V    HS       +FL+KACIK
Sbjct: 263 SIFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKF----RFLDKACIK 322

Query: 325 NQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNTQL 384
            Q + A ESPWRLCT  QV QVKIL+SV+PIFACTI+FNTILAQLQTFSVQQGS MNT +
Sbjct: 323 TQGK-AMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 382

Query: 385 TKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSAFS 444
           TK+F IPPASLQ+IPYI+LI  VPLY+TFFVP ARK TG+ SGISPL+RIG GLFL+ FS
Sbjct: 383 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 442

Query: 445 MVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGMQAF 504
           MV+AA++EKKRR+S +  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFY QS + MQ+F
Sbjct: 443 MVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSF 502

Query: 505 LTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNG---GWLHDNNLNKDRLDLFYWLLAALS 564
           LTAMTYCSYSFGFYLSS+LVS VN++TSS+ +G   GWL DN+LNKDRLD FYWLLA+LS
Sbjct: 503 LTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLS 562

Query: 565 FLNFLNYLFWSKWFDEHPSVS 583
           F+NF NYLFWS+W+   PS +
Sbjct: 563 FINFFNYLFWSRWYSCDPSAT 574

BLAST of CmoCh16G008210 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 406.8 bits (1044), Expect = 3.2e-113
Identity = 238/566 (42.05%), Postives = 342/566 (60.42%), Query Frame = 0

Query: 30  DWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTN 89
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ YL   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 90  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESD 149
           F+GT FLLAL+GGFLSD++  +F   LI   +E  G I+L++QA  P L PP C     D
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-----D 134

Query: 150 GNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTYFNAAY 209
              C E  G KA + FV LYLVALG G +K ++ +HGA+QF   +P   K  ST+FN   
Sbjct: 135 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFV 194

Query: 210 LAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTP 269
              + G L+A+T +VW++ + G + GFGVS   + + ++  ++G  +YRNK P G   T 
Sbjct: 195 FCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTT 254

Query: 270 ILQVFVAALLK------------RKQVCPSNPHMLHGSQTSNDKVGLSS-HSANLIPTQK 329
           IL+V +AA +K               V PSN  +  G +    +  L        +P + 
Sbjct: 255 ILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRA 314

Query: 330 FLNKACIKNQDEAAKESP-WRL--CTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFS 389
            L  + +K  + AA E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL TFS
Sbjct: 315 QLTNS-LKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFS 374

Query: 390 VQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKR 449
           VQQ + MNT++  S  IPPASL   P + ++I+ P+YD   +PFARK T   +G++ L+R
Sbjct: 375 VQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQR 434

Query: 450 IGFGLFLSAFSMVSAAIMEKKRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTA 509
           IG GL LS  +M  AA++E KR+  A     ++  +T  ++  WI  Q+L  G +++FT 
Sbjct: 435 IGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTL 494

Query: 510 VGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLN 569
            GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N
Sbjct: 495 AGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSIN 554

Query: 570 KDRLDLFYWLLAALSFLNFLNYLFWS 573
           + +LD FYWL+  LS  NFL+YLFW+
Sbjct: 555 RYKLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of CmoCh16G008210 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 392.9 bits (1008), Expect = 4.8e-109
Identity = 224/552 (40.58%), Postives = 330/552 (59.78%), Query Frame = 0

Query: 30  DWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTN 89
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ YL N MH SL++S++ VT 
Sbjct: 13  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 72

Query: 90  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESD 149
           F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA  P L PP C    S 
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK--SSA 132

Query: 150 GNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTYFNAAY 209
              C    G KA   FV LYLV+LG G +K ++ +HGA+QF   +P   K  ST+FN   
Sbjct: 133 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYV 192

Query: 210 LAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTP 269
              S G L+A+T +VWI+ + G + GFGVS   + L ++  + G  +Y+NK P+G   T 
Sbjct: 193 FCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTT 252

Query: 270 ILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKVGLSSHSANLIPTQKFLNK-ACIKNQDE 329
           I +V +AA +       S+ H      TS +   + S      P+Q   N   C+    E
Sbjct: 253 IFKVLLAASIVSCSSKTSSNHF-----TSRE---VQSEHEEKTPSQSLTNSLTCLNKAIE 312

Query: 330 AAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSF 389
                 W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  +F
Sbjct: 313 GKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NF 372

Query: 390 HIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSA 449
           ++P ASL   P + ++I+ P YD   +PFARK T    GI+ L+RIG GL LS  +M  A
Sbjct: 373 NVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVA 432

Query: 450 AIMEKKRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGM 509
           A++E KR+  A     ++  +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++   M
Sbjct: 433 ALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSM 492

Query: 510 QAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAALS 569
           ++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  LS
Sbjct: 493 RSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCVLS 552

Query: 570 FLNFLNYLFWSK 574
            +NFL+YLFW+K
Sbjct: 553 VVNFLHYLFWAK 552

BLAST of CmoCh16G008210 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 392.9 bits (1008), Expect = 4.8e-109
Identity = 224/552 (40.58%), Postives = 330/552 (59.78%), Query Frame = 0

Query: 30  DWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSANIVTN 89
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ YL N MH SL++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 90  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCNMLESD 149
           F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA  P L PP C    S 
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK--SSA 136

Query: 150 GNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQSPTQSKTLSTYFNAAY 209
              C    G KA   FV LYLV+LG G +K ++ +HGA+QF   +P   K  ST+FN   
Sbjct: 137 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYV 196

Query: 210 LAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQGPIFTP 269
              S G L+A+T +VWI+ + G + GFGVS   + L ++  + G  +Y+NK P+G   T 
Sbjct: 197 FCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTT 256

Query: 270 ILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKVGLSSHSANLIPTQKFLNK-ACIKNQDE 329
           I +V +AA +       S+ H      TS +   + S      P+Q   N   C+    E
Sbjct: 257 IFKVLLAASIVSCSSKTSSNHF-----TSRE---VQSEHEEKTPSQSLTNSLTCLNKAIE 316

Query: 330 AAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSF 389
                 W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  +F
Sbjct: 317 GKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NF 376

Query: 390 HIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSA 449
           ++P ASL   P + ++I+ P YD   +PFARK T    GI+ L+RIG GL LS  +M  A
Sbjct: 377 NVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVA 436

Query: 450 AIMEKKRRDSA-----VNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIKGM 509
           A++E KR+  A     ++  +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++   M
Sbjct: 437 ALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSM 496

Query: 510 QAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAALS 569
           ++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  LS
Sbjct: 497 RSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCVLS 556

Query: 570 FLNFLNYLFWSK 574
            +NFL+YLFW+K
Sbjct: 557 VVNFLHYLFWAK 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VV54.8e-23174.68Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ65.9e-22170.41Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q8H1574.5e-11242.05Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE46.7e-10840.58Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9M3907.7e-10437.83Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EMI30.0e+0099.68protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JCZ70.0e+0098.05protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1ERH00.0e+0092.86protein NRT1/ PTR FAMILY 4.3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JB170.0e+0091.23protein NRT1/ PTR FAMILY 4.3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1C7K11.1e-30787.86protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146... [more]
Match NameE-valueIdentityDescription
XP_022929221.10.0e+0099.68protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita moschata][more]
KAG7015441.10.0e+0098.87Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_023552004.10.0e+0098.86protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo][more]
XP_022985329.10.0e+0098.05protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita maxima][more]
KAG6577354.10.0e+0097.75Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G59740.13.4e-23274.68Major facilitator superfamily protein [more]
AT1G33440.14.2e-22270.41Major facilitator superfamily protein [more]
AT1G69850.13.2e-11342.05nitrate transporter 1:2 [more]
AT1G27040.24.8e-10940.58Major facilitator superfamily protein [more]
AT1G27040.14.8e-10940.58Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 15..581
e-value: 3.6E-165
score: 552.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 35..574
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 112..539
e-value: 1.7E-90
score: 303.7
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 24..587
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR11654:SF551PROTEIN NRT1/ PTR FAMILY 4.3coord: 24..587
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 44..574
e-value: 0.0
score: 667.575

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G008210.1CmoCh16G008210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity