CmoCh16G006300 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh16G006300
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionWRKY protein
LocationCmo_Chr16: 3100583 .. 3101717 (-)
RNA-Seq ExpressionCmoCh16G006300
SyntenyCmoCh16G006300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCTCCAAACAACAATTTCACAAGATCCCATTTCTCCAATTATTTAAACAAAGTTTGAGCAAATTAAATTTGCTCAAAGCTCTGAACTTTTCAAAGTCAGCCCCCCATGGAGGATTGGGATTTGCAAGCAATTGTAAAAGGATGCAACGGATCAATTACTCCTTCAAGAACAGCAACGCTCATGGAAGATCCAAATTTGGTCTCTTTTTTGATCGAAAAAGATGAACTTTTAAGTTCTTGTTCAGTGTATGATGAGTTTGAAGGGTTGTTTGGGAGCTCTGATGACTCTGCCTCTGCCTCTGCCTCTGCCGCCGCCATTTCTGGTCTTCTCAGAGAGCTTAAGGAACCGGAAAAGCTTCATCGGAAGAGCCAAATCGCTGCAACTAAACAGAAACAGAGGTGGGTACGTCGAATTTAGATTCAAAATTTTATCTTTTGAAGGATTTGATTGAGTTTTGATTTGGTATCAGCAAGAAAAGTCGGGAAAACAGAGTAGTGAAAGAGGTGAAAGCAGAGGGGGTGTGTTCGGATTCATGGGGATGGCGGAAATATGGACAGAAACCGATTAAAGGGTCGCCATATCCGAGAAGCTATTATCGATGTAGCTCCTCCAAAGGATGCTCTGCTAGAAAGCAAGTTGAACGGAGCCTCTCCGATCCTGGCGCCTTTGTCGTCACCTACTCGGCTGAGCACAACCACGCTGAGCCTACTCGCCGTAACGCGCTTGCCGGAACCACCAGGAAGAAGTTTCCGGCGCCGGAAAATCCGAGGTTGGACGTGATTCTGTCGCCTAGTAATTCTATGTCCGTGGCGTCCGTTGAAGAAGAACAGCCGATGGAGGGAGTTGCAGAGGGGGGAGTTATTATGGATTTGCCATTCGAATTCTTTTCTGGTTTGGAGGATTTTCTTTTCGAATGACAAATTTTTTTTTTTTTTTTAAGAGAATGTGAATTGGCAATTGTAATTTGTAAAACTTAATTTTTTTTTTCTTATTTTCATTTTTAATAGAAGTTTTTCAGTTTTGAAAAATGGGCTACCGTGACATGAAAATAACAAGAGGGAAAATGACTCCATCTGCAAATTCTCGGTCAGCCACACGGCTCTGCACGCTTCCGCTATCAACAATGATCTACA

mRNA sequence

AATCTCCAAACAACAATTTCACAAGATCCCATTTCTCCAATTATTTAAACAAAGTTTGAGCAAATTAAATTTGCTCAAAGCTCTGAACTTTTCAAAGTCAGCCCCCCATGGAGGATTGGGATTTGCAAGCAATTGTAAAAGGATGCAACGGATCAATTACTCCTTCAAGAACAGCAACGCTCATGGAAGATCCAAATTTGGTCTCTTTTTTGATCGAAAAAGATGAACTTTTAAGTTCTTGTTCAGTGTATGATGAGTTTGAAGGGTTGTTTGGGAGCTCTGATGACTCTGCCTCTGCCTCTGCCTCTGCCGCCGCCATTTCTGGTCTTCTCAGAGAGCTTAAGGAACCGGAAAAGCTTCATCGGAAGAGCCAAATCGCTGCAACTAAACAGAAACAGAGCAAGAAAAGTCGGGAAAACAGAGTAGTGAAAGAGGTGAAAGCAGAGGGGGTGTGTTCGGATTCATGGGGATGGCGGAAATATGGACAGAAACCGATTAAAGGGTCGCCATATCCGAGAAGCTATTATCGATGTAGCTCCTCCAAAGGATGCTCTGCTAGAAAGCAAGTTGAACGGAGCCTCTCCGATCCTGGCGCCTTTGTCGTCACCTACTCGGCTGAGCACAACCACGCTGAGCCTACTCGCCGTAACGCGCTTGCCGGAACCACCAGGAAGAAGTTTCCGGCGCCGGAAAATCCGAGGTTGGACGTGATTCTGTCGCCTAGTAATTCTATGTCCGTGGCGTCCGTTGAAGAAGAACAGCCGATGGAGGGAGTTGCAGAGGGGGGAGTTATTATGGATTTGCCATTCGAATTCTTTTCTGGTTTGGAGGATTTTCTTTTCGAATGACAAATTTTTTTTTTTTTTTTAAGAGAATGTGAATTGGCAATTGTAATTTGTAAAACTTAATTTTTTTTTTCTTATTTTCATTTTTAATAGAAGTTTTTCAGTTTTGAAAAATGGGCTACCGTGACATGAAAATAACAAGAGGGAAAATGACTCCATCTGCAAATTCTCGGTCAGCCACACGGCTCTGCACGCTTCCGCTATCAACAATGATCTACA

Coding sequence (CDS)

ATGGAGGATTGGGATTTGCAAGCAATTGTAAAAGGATGCAACGGATCAATTACTCCTTCAAGAACAGCAACGCTCATGGAAGATCCAAATTTGGTCTCTTTTTTGATCGAAAAAGATGAACTTTTAAGTTCTTGTTCAGTGTATGATGAGTTTGAAGGGTTGTTTGGGAGCTCTGATGACTCTGCCTCTGCCTCTGCCTCTGCCGCCGCCATTTCTGGTCTTCTCAGAGAGCTTAAGGAACCGGAAAAGCTTCATCGGAAGAGCCAAATCGCTGCAACTAAACAGAAACAGAGCAAGAAAAGTCGGGAAAACAGAGTAGTGAAAGAGGTGAAAGCAGAGGGGGTGTGTTCGGATTCATGGGGATGGCGGAAATATGGACAGAAACCGATTAAAGGGTCGCCATATCCGAGAAGCTATTATCGATGTAGCTCCTCCAAAGGATGCTCTGCTAGAAAGCAAGTTGAACGGAGCCTCTCCGATCCTGGCGCCTTTGTCGTCACCTACTCGGCTGAGCACAACCACGCTGAGCCTACTCGCCGTAACGCGCTTGCCGGAACCACCAGGAAGAAGTTTCCGGCGCCGGAAAATCCGAGGTTGGACGTGATTCTGTCGCCTAGTAATTCTATGTCCGTGGCGTCCGTTGAAGAAGAACAGCCGATGGAGGGAGTTGCAGAGGGGGGAGTTATTATGGATTTGCCATTCGAATTCTTTTCTGGTTTGGAGGATTTTCTTTTCGAATGA

Protein sequence

MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGGVIMDLPFEFFSGLEDFLFE
Homology
BLAST of CmoCh16G006300 vs. ExPASy Swiss-Prot
Match: Q9SUS1 (Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-34
Identity = 101/246 (41.06%), Postives = 138/246 (56.10%), Query Frame = 0

Query: 1   MEDWDLQAIVKGCNGS----------------ITPSRTATLMEDPNLVSFLIEKDELLSS 60
           M++ DL+AIV+G +GS                  P  T++  E     S L E  EL   
Sbjct: 1   MDEGDLEAIVRGYSGSGDAFSGESSGTFSPSFCLPMETSSFYEPEMETSGLDELGEL--- 60

Query: 61  CSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEPEK----LHRKS-----QIAATKQ 120
              Y  F     S+    ++S S    S   R+ K+       L   S     +I+ +K 
Sbjct: 61  ---YKPFYPF--STQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSRADHIRISESKS 120

Query: 121 KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180
           K+SKK+++ RVV++VK E + SD+W WRKYGQKPIKGSPYPRSYYRCSSSKGC ARKQVE
Sbjct: 121 KKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCSSSKGCLARKQVE 180

Query: 181 RSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAP-----ENPRLDVILSPSNSMS 217
           R+  +P  F +TY+ EHNH  PTRRN+LAG+TR K   P     +    +V+ SP+++  
Sbjct: 181 RNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTLTKKSEKEVVSSPTSNPM 238

BLAST of CmoCh16G006300 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 8.3e-33
Identity = 97/270 (35.93%), Postives = 143/270 (52.96%), Query Frame = 0

Query: 2   EDWDLQAIVKGCNGSITPSRTAT-----LMEDPNLVSFLIEKDELLSSCSVYDEFEGLFG 61
           +DWDL A+V+GC+ +++ S T T     +    N +  +  +   +S   + D +     
Sbjct: 3   DDWDLHAVVRGCS-AVSSSATTTVYSPGVSSHTNPIFTVGRQSNAVSFGEIRDLYTPFTQ 62

Query: 62  SSDDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSR------ENRVVKEV 121
            S  S+ +  +        +  K P  L   S    +K   S  SR      +++ V  V
Sbjct: 63  ESVVSSFSCINYPEEPRKPQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHV 122

Query: 122 KAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSA 181
            AE + SD W WRKYGQKPIKGSPYPR YYRCS+SKGC ARKQVER+ SDP  F+VTY+A
Sbjct: 123 AAEALNSDVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTA 182

Query: 182 EHNHAEPTRRNALAGTTRKK-----------------FPAPENPRLDVILSPSNSMSVAS 241
           EHNH  PT RN+LAG+TR+K                   +P     + +L   + ++V  
Sbjct: 183 EHNHPAPTHRNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGD 242

Query: 242 VEEEQPMEGVAEGGVIMDLPFEFFSGLEDF 244
           ++ E+ +  +++  V+ D   +FF GLE+F
Sbjct: 243 LDGEEDLLSLSD-TVVSD---DFFDGLEEF 267

BLAST of CmoCh16G006300 vs. ExPASy Swiss-Prot
Match: Q9FLX8 (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 8.5e-30
Identity = 68/123 (55.28%), Postives = 84/123 (68.29%), Query Frame = 0

Query: 95  QKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 154
           + + +K+++ R +  V  E + SD W WRKYGQKPIKGSPYPR+YYRCSSSKGC ARKQV
Sbjct: 143 RSRKRKNQQKRTICHVTQENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQV 202

Query: 155 ERSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSVASV 214
           ERS  DP  F+VTY+ EH H  PT RN+LAG+TR K   P NP    +  P  S    +V
Sbjct: 203 ERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNK-SQPVNP----VPKPDTSPLSDTV 260

Query: 215 EEE 218
           +EE
Sbjct: 263 KEE 260

BLAST of CmoCh16G006300 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.3e-27
Identity = 69/139 (49.64%), Postives = 87/139 (62.59%), Query Frame = 0

Query: 59  DDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSD 118
           +++ ++S S   IS   R L    +  +  ++       +  SR +        E V SD
Sbjct: 164 NNNNTSSCSQVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSS-------GEVVPSD 223

Query: 119 SWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPT 178
            W WRKYGQKPIKGSPYPR YYRCSSSKGCSARKQVERS +DP   V+TY++EHNH  PT
Sbjct: 224 LWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPT 283

Query: 179 RRNALAGTTRKKFPAPENP 198
           +RNALAG+TR    +  NP
Sbjct: 284 QRNALAGSTRSSSSSSLNP 295

BLAST of CmoCh16G006300 vs. ExPASy Swiss-Prot
Match: Q9FL92 (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.6e-26
Identity = 73/143 (51.05%), Postives = 92/143 (64.34%), Query Frame = 0

Query: 77   ELKEPEKL--HRKSQIAATKQ-------KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQ 136
            E K+ +KL  + +S + ATK+        + K ++E RVV  V      SD W WRKYGQ
Sbjct: 1131 ESKKTDKLVDNIQSSMIATKEIEITRSKSRRKNNKEKRVVCVVDRGSRSSDLWVWRKYGQ 1190

Query: 137  KPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRRNALAGTT 196
            KPIK SPYPRSYYRC+SSKGC ARKQVERS +DP   V+TY +EHNH  PT RN LAG+T
Sbjct: 1191 KPIKSSPYPRSYYRCASSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGST 1250

Query: 197  RKKFPAPENPRLDVILSPSNSMS 211
            R    +  +   DV  S S+++S
Sbjct: 1251 R----SSSSKCSDVTTSASSTVS 1269

BLAST of CmoCh16G006300 vs. ExPASy TrEMBL
Match: A0A6J1EZF1 (probable WRKY transcription factor 29 OS=Cucurbita moschata OX=3662 GN=LOC111437621 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 4.7e-132
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0

Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGGVIMDLPFEFFSGL 240
           NALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGGVIMDLPFEFFSGL
Sbjct: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGGVIMDLPFEFFSGL 240

Query: 241 EDFLFE 247
           EDFLFE
Sbjct: 241 EDFLFE 246

BLAST of CmoCh16G006300 vs. ExPASy TrEMBL
Match: A0A6J1JDJ7 (probable WRKY transcription factor 29 OS=Cucurbita maxima OX=3661 GN=LOC111483427 PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 3.5e-119
Identity = 229/246 (93.09%), Postives = 232/246 (94.31%), Query Frame = 0

Query: 1   MEDWDLQAIVKGCNGSITPSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60
           MEDWDLQAIVKGCNGSIT SRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD
Sbjct: 1   MEDWDLQAIVKGCNGSITSSRTATLMEDPNLVSFLIEKDELLSSCSVYDEFEGLFGSSDD 60

Query: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSDSW 120
           SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSR+NRVVKEVKAEGVCSDSW
Sbjct: 61  SASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRQNRVVKEVKAEGVCSDSW 120

Query: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRR 180
           GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFV+TY+AEHNHAEPTRR
Sbjct: 121 GWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVITYTAEHNHAEPTRR 180

Query: 181 NALAGTTRKKFPAPENPRLDVILSPSNSMSVASVEEEQPMEGVAEGGVIMDLPFEFFSGL 240
           NALAGTTRKKFPAPENPRLD        MSVASVEEEQPME     GVIMDLPFEFFSGL
Sbjct: 181 NALAGTTRKKFPAPENPRLD--------MSVASVEEEQPME-----GVIMDLPFEFFSGL 233

Query: 241 EDFLFE 247
           EDFLFE
Sbjct: 241 EDFLFE 233

BLAST of CmoCh16G006300 vs. ExPASy TrEMBL
Match: E7CEX4 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY26 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.5e-88
Identity = 188/271 (69.37%), Postives = 210/271 (77.49%), Query Frame = 0

Query: 1   MEDWDLQAIVKGCN----GSITPSRTATLMEDPNLVSFL---IEKDELLSSC-------- 60
           MEDW LQAIVKGCN    GS T + T  LMED N  SFL    E+DE  SSC        
Sbjct: 1   MEDWGLQAIVKGCNGIPIGSSTTAATTRLMEDSNWYSFLRSDQEEDEFFSSCVYNSSYNN 60

Query: 61  -------SVYDEFEGLFGSSDDSASASASAAAISGLLRELKEP--EKLHRKSQ-IAATKQ 120
                    +DEFEGLFG +  + SA   AA+IS LLR+ KEP  +KLH K+Q I  TKQ
Sbjct: 61  PQISSSSIFHDEFEGLFGRNSSNNSA---AASISHLLRDFKEPADQKLHHKNQIIQPTKQ 120

Query: 121 KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180
           KQSKKSR+NRVVKEVKA+ VCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE
Sbjct: 121 KQSKKSRQNRVVKEVKADKVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180

Query: 181 RSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSVASVE 240
           RSLSDP  F+VTY+AEHNHAEPTRRNALAGTTRKKFPA ENP LD+ILSP+NS SVAS+E
Sbjct: 181 RSLSDPEVFIVTYTAEHNHAEPTRRNALAGTTRKKFPALENPNLDMILSPNNSTSVASIE 240

Query: 241 EEQ-PMEGVAEGGVIMDLPFEFFSGLEDFLF 246
           E+Q PMEGV +G V++D+PFEFF+GLED LF
Sbjct: 241 EDQHPMEGVTDGEVLIDMPFEFFTGLEDLLF 268

BLAST of CmoCh16G006300 vs. ExPASy TrEMBL
Match: A0A5D3CZ98 (Putative WRKY transcription factor 29 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004460 PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.5e-87
Identity = 189/273 (69.23%), Postives = 209/273 (76.56%), Query Frame = 0

Query: 1   MEDWDLQAIVKGCN----GSITPSRTATLMEDP-NLVSFLIEKDE--------------- 60
           MEDW LQAIVKGCN    GS T + T  LMED  NL SFL    E               
Sbjct: 1   MEDWGLQAIVKGCNGIPIGSSTTAATTRLMEDTNNLYSFLRSDQEEDGGFFSSCVYNNYY 60

Query: 61  ---LLSSCSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEP--EKLHRKSQ-IAATK 120
              + SS   +DEFEGLFG +  + SA   AA+IS LLR+ KEP  +KLH K+Q I  TK
Sbjct: 61  NPQISSSSIFHDEFEGLFGRNSSNNSA---AASISHLLRDFKEPADQKLHHKNQIIQPTK 120

Query: 121 QKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 180
           QKQSKKSR+NRVVKEVKA+ VCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV
Sbjct: 121 QKQSKKSRQNRVVKEVKADKVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 180

Query: 181 ERSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSVASV 240
           ERSLSDP  FVVTY+AEHNHAEPTRRNALAGTTRKKFPA ENP LD+ILSP+NS SVAS+
Sbjct: 181 ERSLSDPEVFVVTYTAEHNHAEPTRRNALAGTTRKKFPALENPNLDMILSPNNSTSVASI 240

Query: 241 EEEQ--PMEGVAEGGVIMDLPFEFFSGLEDFLF 246
           EE+Q  PMEGVA+G V+MD+PFEFF+GLED LF
Sbjct: 241 EEDQHHPMEGVADGEVLMDMPFEFFTGLEDLLF 270

BLAST of CmoCh16G006300 vs. ExPASy TrEMBL
Match: A0A1S3BTM4 (probable WRKY transcription factor 29 OS=Cucumis melo OX=3656 GN=LOC103493141 PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.5e-87
Identity = 189/273 (69.23%), Postives = 209/273 (76.56%), Query Frame = 0

Query: 1   MEDWDLQAIVKGCN----GSITPSRTATLMEDP-NLVSFLIEKDE--------------- 60
           MEDW LQAIVKGCN    GS T + T  LMED  NL SFL    E               
Sbjct: 1   MEDWGLQAIVKGCNGIPIGSSTTAATTRLMEDTNNLYSFLRSDQEEDGGFFSSCVYNNYY 60

Query: 61  ---LLSSCSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEP--EKLHRKSQ-IAATK 120
              + SS   +DEFEGLFG +  + SA   AA+IS LLR+ KEP  +KLH K+Q I  TK
Sbjct: 61  NPQISSSSIFHDEFEGLFGRNSSNNSA---AASISHLLRDFKEPADQKLHHKNQIIQPTK 120

Query: 121 QKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 180
           QKQSKKSR+NRVVKEVKA+ VCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV
Sbjct: 121 QKQSKKSRQNRVVKEVKADKVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 180

Query: 181 ERSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSVASV 240
           ERSLSDP  FVVTY+AEHNHAEPTRRNALAGTTRKKFPA ENP LD+ILSP+NS SVAS+
Sbjct: 181 ERSLSDPEVFVVTYTAEHNHAEPTRRNALAGTTRKKFPALENPNLDMILSPNNSTSVASI 240

Query: 241 EEEQ--PMEGVAEGGVIMDLPFEFFSGLEDFLF 246
           EE+Q  PMEGVA+G V+MD+PFEFF+GLED LF
Sbjct: 241 EEDQHHPMEGVADGEVLMDMPFEFFTGLEDLLF 270

BLAST of CmoCh16G006300 vs. TAIR 10
Match: AT4G23550.1 (WRKY family transcription factor )

HSP 1 Score: 148.3 bits (373), Expect = 8.2e-36
Identity = 101/246 (41.06%), Postives = 138/246 (56.10%), Query Frame = 0

Query: 1   MEDWDLQAIVKGCNGS----------------ITPSRTATLMEDPNLVSFLIEKDELLSS 60
           M++ DL+AIV+G +GS                  P  T++  E     S L E  EL   
Sbjct: 1   MDEGDLEAIVRGYSGSGDAFSGESSGTFSPSFCLPMETSSFYEPEMETSGLDELGEL--- 60

Query: 61  CSVYDEFEGLFGSSDDSASASASAAAISGLLRELKEPEK----LHRKS-----QIAATKQ 120
              Y  F     S+    ++S S    S   R+ K+       L   S     +I+ +K 
Sbjct: 61  ---YKPFYPF--STQTILTSSVSLPEDSKPFRDDKKQRSHGCLLSNGSRADHIRISESKS 120

Query: 121 KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVE 180
           K+SKK+++ RVV++VK E + SD+W WRKYGQKPIKGSPYPRSYYRCSSSKGC ARKQVE
Sbjct: 121 KKSKKNQQKRVVEQVKEENLLSDAWAWRKYGQKPIKGSPYPRSYYRCSSSKGCLARKQVE 180

Query: 181 RSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAP-----ENPRLDVILSPSNSMS 217
           R+  +P  F +TY+ EHNH  PTRRN+LAG+TR K   P     +    +V+ SP+++  
Sbjct: 181 RNPQNPEKFTITYTNEHNHELPTRRNSLAGSTRAKTSQPKPTLTKKSEKEVVSSPTSNPM 238

BLAST of CmoCh16G006300 vs. TAIR 10
Match: AT4G01250.1 (WRKY family transcription factor )

HSP 1 Score: 142.1 bits (357), Expect = 5.9e-34
Identity = 97/270 (35.93%), Postives = 143/270 (52.96%), Query Frame = 0

Query: 2   EDWDLQAIVKGCNGSITPSRTAT-----LMEDPNLVSFLIEKDELLSSCSVYDEFEGLFG 61
           +DWDL A+V+GC+ +++ S T T     +    N +  +  +   +S   + D +     
Sbjct: 3   DDWDLHAVVRGCS-AVSSSATTTVYSPGVSSHTNPIFTVGRQSNAVSFGEIRDLYTPFTQ 62

Query: 62  SSDDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSR------ENRVVKEV 121
            S  S+ +  +        +  K P  L   S    +K   S  SR      +++ V  V
Sbjct: 63  ESVVSSFSCINYPEEPRKPQNQKRPLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHV 122

Query: 122 KAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSA 181
            AE + SD W WRKYGQKPIKGSPYPR YYRCS+SKGC ARKQVER+ SDP  F+VTY+A
Sbjct: 123 AAEALNSDVWAWRKYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTA 182

Query: 182 EHNHAEPTRRNALAGTTRKK-----------------FPAPENPRLDVILSPSNSMSVAS 241
           EHNH  PT RN+LAG+TR+K                   +P     + +L   + ++V  
Sbjct: 183 EHNHPAPTHRNSLAGSTRQKPSDQQTSKSPTTTIATYSSSPVTSADEFVLPVEDHLAVGD 242

Query: 242 VEEEQPMEGVAEGGVIMDLPFEFFSGLEDF 244
           ++ E+ +  +++  V+ D   +FF GLE+F
Sbjct: 243 LDGEEDLLSLSD-TVVSD---DFFDGLEEF 267

BLAST of CmoCh16G006300 vs. TAIR 10
Match: AT5G52830.1 (WRKY DNA-binding protein 27 )

HSP 1 Score: 132.1 bits (331), Expect = 6.1e-31
Identity = 68/123 (55.28%), Postives = 84/123 (68.29%), Query Frame = 0

Query: 95  QKQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQV 154
           + + +K+++ R +  V  E + SD W WRKYGQKPIKGSPYPR+YYRCSSSKGC ARKQV
Sbjct: 143 RSRKRKNQQKRTICHVTQENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSKGCLARKQV 202

Query: 155 ERSLSDPGAFVVTYSAEHNHAEPTRRNALAGTTRKKFPAPENPRLDVILSPSNSMSVASV 214
           ERS  DP  F+VTY+ EH H  PT RN+LAG+TR K   P NP    +  P  S    +V
Sbjct: 203 ERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNK-SQPVNP----VPKPDTSPLSDTV 260

Query: 215 EEE 218
           +EE
Sbjct: 263 KEE 260

BLAST of CmoCh16G006300 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 124.0 bits (310), Expect = 1.7e-28
Identity = 69/139 (49.64%), Postives = 87/139 (62.59%), Query Frame = 0

Query: 59  DDSASASASAAAISGLLRELKEPEKLHRKSQIAATKQKQSKKSRENRVVKEVKAEGVCSD 118
           +++ ++S S   IS   R L    +  +  ++       +  SR +        E V SD
Sbjct: 164 NNNNTSSCSQVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSRSS-------GEVVPSD 223

Query: 119 SWGWRKYGQKPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPT 178
            W WRKYGQKPIKGSPYPR YYRCSSSKGCSARKQVERS +DP   V+TY++EHNH  PT
Sbjct: 224 LWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPT 283

Query: 179 RRNALAGTTRKKFPAPENP 198
           +RNALAG+TR    +  NP
Sbjct: 284 QRNALAGSTRSSSSSSLNP 295

BLAST of CmoCh16G006300 vs. TAIR 10
Match: AT5G45050.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 120.6 bits (301), Expect = 1.8e-27
Identity = 73/143 (51.05%), Postives = 92/143 (64.34%), Query Frame = 0

Query: 77   ELKEPEKL--HRKSQIAATKQ-------KQSKKSRENRVVKEVKAEGVCSDSWGWRKYGQ 136
            E K+ +KL  + +S + ATK+        + K ++E RVV  V      SD W WRKYGQ
Sbjct: 1131 ESKKTDKLVDNIQSSMIATKEIEITRSKSRRKNNKEKRVVCVVDRGSRSSDLWVWRKYGQ 1190

Query: 137  KPIKGSPYPRSYYRCSSSKGCSARKQVERSLSDPGAFVVTYSAEHNHAEPTRRNALAGTT 196
            KPIK SPYPRSYYRC+SSKGC ARKQVERS +DP   V+TY +EHNH  PT RN LAG+T
Sbjct: 1191 KPIKSSPYPRSYYRCASSKGCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGST 1250

Query: 197  RKKFPAPENPRLDVILSPSNSMS 211
            R    +  +   DV  S S+++S
Sbjct: 1251 R----SSSSKCSDVTTSASSTVS 1269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUS11.2e-3441.06Probable WRKY transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=WRKY29 ... [more]
O046098.3e-3335.93WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
Q9FLX88.5e-3055.28Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ... [more]
O647472.3e-2749.64Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
Q9FL922.6e-2651.05Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1EZF14.7e-132100.00probable WRKY transcription factor 29 OS=Cucurbita moschata OX=3662 GN=LOC111437... [more]
A0A6J1JDJ73.5e-11993.09probable WRKY transcription factor 29 OS=Cucurbita maxima OX=3661 GN=LOC11148342... [more]
E7CEX42.5e-8869.37WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY26 PE=2 SV=1[more]
A0A5D3CZ983.5e-8769.23Putative WRKY transcription factor 29 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BTM43.5e-8769.23probable WRKY transcription factor 29 OS=Cucumis melo OX=3656 GN=LOC103493141 PE... [more]
Match NameE-valueIdentityDescription
AT4G23550.18.2e-3641.06WRKY family transcription factor [more]
AT4G01250.15.9e-3435.93WRKY family transcription factor [more]
AT5G52830.16.1e-3155.28WRKY DNA-binding protein 27 [more]
AT2G34830.11.7e-2849.64WRKY DNA-binding protein 35 [more]
AT5G45050.11.8e-2751.05Disease resistance protein (TIR-NBS-LRR class) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 116..176
e-value: 4.3E-34
score: 129.3
IPR003657WRKY domainPFAMPF03106WRKYcoord: 118..174
e-value: 4.0E-25
score: 87.8
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 111..177
score: 32.225159
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 93..178
e-value: 2.6E-28
score: 99.6
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 114..177
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..105
NoneNo IPR availablePANTHERPTHR32096:SF87WRKY TRANSCRIPTION FACTOR 63-RELATEDcoord: 3..210
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 3..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh16G006300.1CmoCh16G006300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0043565 sequence-specific DNA binding