CmoCh15G014820 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G014820
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionCytochrome p450 76c2
LocationCmo_Chr15: 10052721 .. 10054866 (+)
RNA-Seq ExpressionCmoCh15G014820
SyntenyCmoCh15G014820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATTGCTTCATTTTCCTTTCTTCTTCTTCTACCTCTAATCTTCGCCATTCTTAAGCACATCAGTTCATCTTCAAAACAGCCGCCATTTCCTCCGGGTCCAAAGCCATGGCCGATGCTTGGAAATCTCCTTCAGATCGGTCAAAACGCCCACATTTCAATGACCCAATTCTCTCATATCTACGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGCTTGGTGGTCGCTTCTTCACCGGCCGCCGCCGCCGCCATTCTCAAGACTCAAGATCGCCTTCTATCTGCTCGATACATTTTCCAAATGATACCTGATCGAGCTCTGCACGATAACTGCTCGCTTGTTTTCTCACCGGAATGCGGCGATCGTTGGAAGAATCTGAGATCCATTTGCAGAGCCAAATTGTTTACAGCGAGAGCGATCGAATCCCAAGCCAGTTTGAGAAGGAAGAAGATGAATGAAATGGTGGAGTTCTTGCAGTCAAAACAGGGATCTGTGGTGGAGATTAAAGAATTTGTGTTTATTACTGTTTTCAACACTCTTTCTAACTTCATCTTCTCTAGGGATTTGGTTGGTTTTGTGGGTGATGGGTTCAATGGGATTGAAGGGCATTTCAAGAACGTGATCGATTGGGCTTTGATCCCAAATTTGGCTGACTTTTACCCTTTGCTCAGATGCTTCGATCTTCAAGGACTCCGAAAGAAAGCTGCTATCTATAAGAACCAAATCGATTCTTCGTGGGAAATTCTTATTAAAGAACGAAGAACAATTCATAGTCGAGGCGTTTTAGTTACGAGCGATTTCTTGGATGTTTTGATCCAAAGTGGATTCACCGATGAACAAATTAACTACTTGATTGTTGTAAGTGAAAAAAATCATCGAGGGAGTCTCACACTAATGGGTACTATGCATGTCTAGTGGTAGGAGGTATTTCGGGAAGCTCAAAGAAAAGTCACCGAGTCTATGCTCAAAGTGAACAATATATCATATCATTGTAGAGAGTCGTGATTCCTAATATGGTATCAGAATCATGCCCTTAACTTAGTCATGTCAATAGAATTCTCAAATGTCAAACAAAAAAGTTGTGAGGCTTGAAAGTATAGTCAAAAGTGACTTAAGTGTCGAACAAAGGGTATAATTTGTTCGATGACTCTAGAAAAGGAGTCGTGCCTCGATTAAGGAGAGGTTGTTCGAAAACTTCATAATGCTCACGAGATACTCTACAGTGTACTTTGTTCGAGAGGAGAATTGTTGGGAGGGAGTCCCATATTGACTAATTTAGGGGTATACTTTGTTTGATGAGCTCGGATTAAGGGGGACTTCAATATAAGTAAGGAATGTATCTCCACTTGTGTGAGTCTTTTTTGGGAAAGCCCAAAAGAAAGTCACTAGAGCTTATGTTCAAGGTGGATAATATCATACTATCGAGCCAGAGTTGTGATTACTAACGAAGGAACGAACAAAAAAAAAAAAAAAAAAACTAAATTTTTTTTTAGATGGATAGTTTTCTAATCAAAGTTTTGATGGGTTATTGCAGGAAGTGTTGTCCGCCGGCACGGATACAACGACCACGACGGTGGAGTGGGCGATGGCGGAGCTGTTGAAGAACAGAGACATTATGAACAGAGTTCGAGAGGAAACAAAGAAGGCAATCAACGGAGACGCCATTGACGAATCCAAACTCTCTGAGCTTCATTATCTATGGAAATGCGTGAAAGAAACTCTACGGCTGCATCCGCCTGCGCCATTTCTTCTTCCACGGTATGCTCCAGAAGCTTGTGAGCTTATGGGGTACTCTGTTCCTAAACACACCATGATTGTCGTGAATGTTTGGGGGATTGGGCGAGACCCTTCAATCTGGGAAGATCCTCAATCGTTCAAACCAGAAAGATTTGATGTCTGTGATGCAGATTTGAAGGGTCATGATTATCGATTCCTGCCATTTGGAGGAGGCAGAAGGCTTTGCCCTGGGCTTCCCATGGCGGCGGTGCAAGTGCCACTAATCTTAGCCACCTTAATTCACAACTTTGAATGGTGCCTTCCTAATGATGAGGATGCGTCAACGCTGGATTTGAAAGGAAGGCTTGGTGTAACATTACAAAAGGAAGAACCTCTTAAGCTTCTTCCTAAAAGGAGGATTTAA

mRNA sequence

ATGGAGATTGCTTCATTTTCCTTTCTTCTTCTTCTACCTCTAATCTTCGCCATTCTTAAGCACATCAGTTCATCTTCAAAACAGCCGCCATTTCCTCCGGGTCCAAAGCCATGGCCGATGCTTGGAAATCTCCTTCAGATCGGTCAAAACGCCCACATTTCAATGACCCAATTCTCTCATATCTACGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGCTTGGTGGTCGCTTCTTCACCGGCCGCCGCCGCCGCCATTCTCAAGACTCAAGATCGCCTTCTATCTGCTCGATACATTTTCCAAATGATACCTGATCGAGCTCTGCACGATAACTGCTCGCTTGTTTTCTCACCGGAATGCGGCGATCGTTGGAAGAATCTGAGATCCATTTGCAGAGCCAAATTGTTTACAGCGAGAGCGATCGAATCCCAAGCCAGTTTGAGAAGGAAGAAGATGAATGAAATGGTGGAGTTCTTGCAGTCAAAACAGGGATCTGTGGTGGAGATTAAAGAATTTGTGTTTATTACTGTTTTCAACACTCTTTCTAACTTCATCTTCTCTAGGGATTTGGTTGGTTTTGTGGGTGATGGGTTCAATGGGATTGAAGGGCATTTCAAGAACGTGATCGATTGGGCTTTGATCCCAAATTTGGCTGACTTTTACCCTTTGCTCAGATGCTTCGATCTTCAAGGACTCCGAAAGAAAGCTGCTATCTATAAGAACCAAATCGATTCTTCGTGGGAAATTCTTATTAAAGAACGAAGAACAATTCATAGTCGAGGCGTTTTAGTTACGAGCGATTTCTTGGATGTTTTGATCCAAAGTGGATTCACCGATGAACAAATTAACTACTTGATTGTTGAAGTGTTGTCCGCCGGCACGGATACAACGACCACGACGGTGGAGTGGGCGATGGCGGAGCTGTTGAAGAACAGAGACATTATGAACAGAGTTCGAGAGGAAACAAAGAAGGCAATCAACGGAGACGCCATTGACGAATCCAAACTCTCTGAGCTTCATTATCTATGGAAATGCGTGAAAGAAACTCTACGGCTGCATCCGCCTGCGCCATTTCTTCTTCCACGGTATGCTCCAGAAGCTTGTGAGCTTATGGGGTACTCTGTTCCTAAACACACCATGATTGTCGTGAATGTTTGGGGGATTGGGCGAGACCCTTCAATCTGGGAAGATCCTCAATCGTTCAAACCAGAAAGATTTGATGTCTGTGATGCAGATTTGAAGGGTCATGATTATCGATTCCTGCCATTTGGAGGAGGCAGAAGGCTTTGCCCTGGGCTTCCCATGGCGGCGGTGCAAGTGCCACTAATCTTAGCCACCTTAATTCACAACTTTGAATGGTGCCTTCCTAATGATGAGGATGCGTCAACGCTGGATTTGAAAGGAAGGCTTGGTGTAACATTACAAAAGGAAGAACCTCTTAAGCTTCTTCCTAAAAGGAGGATTTAA

Coding sequence (CDS)

ATGGAGATTGCTTCATTTTCCTTTCTTCTTCTTCTACCTCTAATCTTCGCCATTCTTAAGCACATCAGTTCATCTTCAAAACAGCCGCCATTTCCTCCGGGTCCAAAGCCATGGCCGATGCTTGGAAATCTCCTTCAGATCGGTCAAAACGCCCACATTTCAATGACCCAATTCTCTCATATCTACGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGCTTGGTGGTCGCTTCTTCACCGGCCGCCGCCGCCGCCATTCTCAAGACTCAAGATCGCCTTCTATCTGCTCGATACATTTTCCAAATGATACCTGATCGAGCTCTGCACGATAACTGCTCGCTTGTTTTCTCACCGGAATGCGGCGATCGTTGGAAGAATCTGAGATCCATTTGCAGAGCCAAATTGTTTACAGCGAGAGCGATCGAATCCCAAGCCAGTTTGAGAAGGAAGAAGATGAATGAAATGGTGGAGTTCTTGCAGTCAAAACAGGGATCTGTGGTGGAGATTAAAGAATTTGTGTTTATTACTGTTTTCAACACTCTTTCTAACTTCATCTTCTCTAGGGATTTGGTTGGTTTTGTGGGTGATGGGTTCAATGGGATTGAAGGGCATTTCAAGAACGTGATCGATTGGGCTTTGATCCCAAATTTGGCTGACTTTTACCCTTTGCTCAGATGCTTCGATCTTCAAGGACTCCGAAAGAAAGCTGCTATCTATAAGAACCAAATCGATTCTTCGTGGGAAATTCTTATTAAAGAACGAAGAACAATTCATAGTCGAGGCGTTTTAGTTACGAGCGATTTCTTGGATGTTTTGATCCAAAGTGGATTCACCGATGAACAAATTAACTACTTGATTGTTGAAGTGTTGTCCGCCGGCACGGATACAACGACCACGACGGTGGAGTGGGCGATGGCGGAGCTGTTGAAGAACAGAGACATTATGAACAGAGTTCGAGAGGAAACAAAGAAGGCAATCAACGGAGACGCCATTGACGAATCCAAACTCTCTGAGCTTCATTATCTATGGAAATGCGTGAAAGAAACTCTACGGCTGCATCCGCCTGCGCCATTTCTTCTTCCACGGTATGCTCCAGAAGCTTGTGAGCTTATGGGGTACTCTGTTCCTAAACACACCATGATTGTCGTGAATGTTTGGGGGATTGGGCGAGACCCTTCAATCTGGGAAGATCCTCAATCGTTCAAACCAGAAAGATTTGATGTCTGTGATGCAGATTTGAAGGGTCATGATTATCGATTCCTGCCATTTGGAGGAGGCAGAAGGCTTTGCCCTGGGCTTCCCATGGCGGCGGTGCAAGTGCCACTAATCTTAGCCACCTTAATTCACAACTTTGAATGGTGCCTTCCTAATGATGAGGATGCGTCAACGCTGGATTTGAAAGGAAGGCTTGGTGTAACATTACAAAAGGAAGAACCTCTTAAGCTTCTTCCTAAAAGGAGGATTTAA

Protein sequence

MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLKLLPKRRI
Homology
[Close]
BLAST of CmoCh15G014820 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.0e-119
Identity = 227/488 (46.52%), Postives = 310/488 (63.52%), Query Frame = 0 

Query: 3   IASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIY 62
           I SFSFLL L   F IL+     S+    PPGP+P P++GNLLQ+G   H    + +  Y
Sbjct: 8   IVSFSFLLFL---FFILR----DSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 67

Query: 63  GPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECG 122
           G L SLKLG+Q +VVASSPAAAA ILKT D++LS RY+FQ    +  H   S+V+S EC 
Sbjct: 68  GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENSIVWS-ECN 127

Query: 123 DRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTL 182
           D WK LR +CR +LFT + IESQ+ +R  K  EMV+FL+ K+G VV+I E VF T+ N  
Sbjct: 128 DNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIF 187

Query: 183 SNFIFSRDLVGFVGDGFNGIE--GHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 242
            N IFS+D+          +E   H   ++D     N AD++P++   DL G R+  A  
Sbjct: 188 GNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEV 247

Query: 243 KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT 302
             QI   W +++KERR   ++G    +DF+DVL+ +G  D++IN L++E+  AGT+T+ +
Sbjct: 248 LQQIYDVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSAS 307

Query: 303 TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL 362
           T+EWA+ EL K   +++++R E    +  + + ES L  L YL   VKETLRLHPP P L
Sbjct: 308 TIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLL 367

Query: 363 LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR 422
           LPR A E C +M Y++PK   I+VN W IGRDP  W+DP +FKPERF   D D KG+D+ 
Sbjct: 368 LPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFE 427

Query: 423 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP 482
            +PFGGGRR+CPGLP+A+    LI+ATL+ NFEW LP     S L +  + G+TLQK+ P
Sbjct: 428 LIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPP 484

Query: 483 LKLLPKRR 489
           L ++ K R
Sbjct: 488 LLIVLKAR 484

[Close]
BLAST of CmoCh15G014820 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.4e-116
Identity = 215/492 (43.70%), Postives = 310/492 (63.01%), Query Frame = 0 

Query: 1   MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH 60
           ME+ + +  L+  +I +IL  +  SS     PPGPKPWP++GNLLQ+G+  H    + + 
Sbjct: 1   MEVVTVA--LIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQ 60

Query: 61  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 120
            YG + +LK+G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +
Sbjct: 61  TYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-D 120

Query: 121 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 180
           C + WKNLR +CR +LFT + IESQA +R KK  EMVE+L  KQG  V+I E +F T+ N
Sbjct: 121 CTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVN 180

Query: 181 TLSNFIFSRDL--VGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAA 240
              N IFS+++  +G    G +  + +   +++     N AD++P+L  FDL G RK+ A
Sbjct: 181 IFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVA 240

Query: 241 IYKNQIDSSWEILIKERRTIHS-RGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDT 300
                I + W  +++ER+      G    +DF+DV + SG  D QIN L++E+  AGT+T
Sbjct: 241 ECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTET 300

Query: 301 TTTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPA 360
           + +T+EWAM EL KN  I  ++R E +  +   ++ ES    L YL   VKETLRLHPP 
Sbjct: 301 SASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPT 360

Query: 361 PFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGH 420
           P LLPR A E C ++ Y++PK   I+VN WGIGRDP  W DP +F PERF     D +G+
Sbjct: 361 PLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGN 420

Query: 421 DYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQK 480
           D+  +PFG GRR+CPGLP+A   + L++AT + N +WCLPN      L ++ + G+TLQK
Sbjct: 421 DFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQK 480

Query: 481 EEPLKLLPKRRI 490
           E PL ++PK R+
Sbjct: 481 EPPLFIVPKSRV 488

[Close]
BLAST of CmoCh15G014820 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.8e-116
Identity = 212/483 (43.89%), Postives = 304/483 (62.94%), Query Frame = 0 

Query: 10  LLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 69
           L+  +I +IL  +   S     PPGPKPWP++GNLLQ+G+  H    + +  YG + +LK
Sbjct: 7   LIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLK 66

Query: 70  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 129
           +G + +VVAS+ +AA+ ILKT DR+LSARY+FQ    +  H   S+V+S +C + WKNLR
Sbjct: 67  MGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLR 126

Query: 130 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 189
            +CR +LFT + IESQA +R KK  EMVE+L  KQG  V+I E +F T+ N   N IFS+
Sbjct: 127 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 186

Query: 190 DL--VGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 249
           ++  +G    G +  + +   +++     N AD++P+L  FDL G RK+ A     I + 
Sbjct: 187 NIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAI 246

Query: 250 WEILIKERRTIHS-RGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAM 309
           W  +++ER+      G    +DF+DV + SG  D QIN L++E+  AGT+T+ +T+EWAM
Sbjct: 247 WGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 306

Query: 310 AELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 369
            EL KN  I  ++R E +  +   ++ ES    L YL   VKETLRLHPP P LLPR A 
Sbjct: 307 TELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRAL 366

Query: 370 EACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFLPFGG 429
           E C ++ Y++PK   I+VN WGIGRDP  W DP +F PERF     D +G+D+  +PFG 
Sbjct: 367 ETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFGA 426

Query: 430 GRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLKLLPK 489
           GRR+CPGLP+A   + L++AT + N +WCLPN      L ++ + G+TLQKE PL ++PK
Sbjct: 427 GRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPK 486

[Close]
BLAST of CmoCh15G014820 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.5e-113
Identity = 210/481 (43.66%), Postives = 296/481 (61.54%), Query Frame = 0 

Query: 10  LLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLISLK 69
           L+  +I   L  I   S     PPGPKPWP++GNLLQ+G+  H    Q +  YG L SLK
Sbjct: 2   LVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLK 61

Query: 70  LGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLR 129
           LG++ +VVAS+P AA+ ILKT DR+LS RY+FQ    +  H   S+V+S EC + WK LR
Sbjct: 62  LGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNETWKKLR 121

Query: 130 SICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFIFSR 189
            +CR +LFT + IESQA +R  K  EMVE+L+   G+ V+I E VF T+ N   N IFS+
Sbjct: 122 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 181

Query: 190 DL--VGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSS 249
           ++  +G    G   ++ H   +++     N AD++P L  FDL G  K  A     I S 
Sbjct: 182 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSV 241

Query: 250 WEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAMA 309
           W  ++KER+          +DF+++L+ SG  D+QIN L++E+  AGT+T+ +T+EWA++
Sbjct: 242 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 301

Query: 310 ELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPE 369
           EL KN  +   +R E    +    + ES +  + YL   VKETLRLHP  P LLPR A E
Sbjct: 302 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 361

Query: 370 ACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYRFLPFGGG 429
            C+++ Y++PK   I+VN WGIGRDP  W DP  F PERF     D KG+D+  +PFG G
Sbjct: 362 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 421

Query: 430 RRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLKLLPKR 489
           RR+CPG+P+A   + LI+++L+ NF+W  P   D S L ++ + G+TLQKE PL ++PK 
Sbjct: 422 RRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 480

[Close]
BLAST of CmoCh15G014820 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.0e-103
Identity = 202/494 (40.89%), Postives = 306/494 (61.94%), Query Frame = 0 

Query: 1   MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNA----HISMT 60
           ++IA FS + ++ +   +LK      K    PPGP PWP++GNL  +  N     HI++ 
Sbjct: 3   LQIALFSLIPVILVFILLLK-----PKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLA 62

Query: 61  QFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLV 120
             +  +GP++ L LG Q  V+AS+  AA  ILKT DR+ SAR+I +M      H   SLV
Sbjct: 63  NMARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLV 122

Query: 121 FSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFI 180
           +S +C D WK LR I R ++F+ + +++Q+ +R +K+ E+++FL+SK+G VV+I +FVF 
Sbjct: 123 WS-DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFG 182

Query: 181 TVFNTLSNFIFSRDLVGFVGDGFN--GIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLR 240
           T+ N L N +FS+D+  + GD  +  GI+   + ++     PN+A+FYP L   DLQGL+
Sbjct: 183 TLLNILGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLK 242

Query: 241 KKAAIYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAG 300
           KK      ++   WE  +KER+   +     + D LDVL+ + F D QIN L +E    G
Sbjct: 243 KKCDERFIRVMKMWEGTVKERKANRNE---ESKDMLDVLLANDFNDAQINALFLETFGPG 302

Query: 301 TDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLH 360
           ++T++ T+EW +AEL+K+   M +VR+E  + +    I ES L +L YL  C+KE +RLH
Sbjct: 303 SETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLH 362

Query: 361 PPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADL 420
           P APFLLPR A E CE+MGY++PK++ ++VN + IGRDP  W+DP +F PERF   D D 
Sbjct: 363 PAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDF 422

Query: 421 KGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVT 480
            G  Y+F+PFG GRR C G+P+A   +PLI+ +L+HN+++ LP       L +   L +T
Sbjct: 423 HGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLT 482

Query: 481 LQKEEPLKLLPKRR 489
           L  +  L ++PK R
Sbjct: 483 LAIDPSLCVVPKAR 485

[Close]
BLAST of CmoCh15G014820 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.4e-284
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0 

Query: 1   MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH 60
           MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH
Sbjct: 1   MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH 60

Query: 61  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 120
           IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE
Sbjct: 61  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 120

Query: 121 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 180
           CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN
Sbjct: 121 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 180

Query: 181 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 240
           TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY
Sbjct: 181 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 240

Query: 241 KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT 300
           KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT
Sbjct: 241 KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT 300

Query: 301 TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL 360
           TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL
Sbjct: 301 TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL 360

Query: 361 LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR 420
           LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR
Sbjct: 361 LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR 420

Query: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP 480
           FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP
Sbjct: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP 480

Query: 481 LKLLPKRRI 490
           LKLLPKRRI
Sbjct: 481 LKLLPKRRI 489

[Close]
BLAST of CmoCh15G014820 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 7.6e-267
Identity = 462/489 (94.48%), Postives = 471/489 (96.32%), Query Frame = 0 

Query: 1   MEIASFSF-LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFS 60
           MEIASFSF LLLLPLIF IL+HISSSSKQPP PPGPKPWP+LGNLLQIGQNAHISMTQFS
Sbjct: 1   MEIASFSFLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFS 60

Query: 61  HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSP 120
           HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FSP
Sbjct: 61  HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSP 120

Query: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVF 180
           ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKE VFITVF
Sbjct: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVF 180

Query: 181 NTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAI 240
           NTLSNFIFSRDLVGFVGDGFNGI+G FKN+ID  LIPNLADFYPLLRCFDLQGLRKKAAI
Sbjct: 181 NTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAI 240

Query: 241 YKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTT 300
           YKNQ+DSSWEILIKERRTIHS+GV   SDFLDVLIQSGFTDEQI+YLIVEVLSAGTDTTT
Sbjct: 241 YKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTT 300

Query: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPF 360
           TTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAPF
Sbjct: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPF 360

Query: 361 LLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDY 420
           LLPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD CDADLKGHDY
Sbjct: 361 LLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDY 420

Query: 421 RFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEE 480
           RFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDL GRLGVTLQKEE
Sbjct: 421 RFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEE 480

Query: 481 PLKLLPKRR 489
           PLKLL K R
Sbjct: 481 PLKLLSKNR 489

[Close]
BLAST of CmoCh15G014820 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.6e-259
Identity = 452/489 (92.43%), Postives = 463/489 (94.68%), Query Frame = 0 

Query: 1   MEIASFSF-LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFS 60
           MEIASFSF LLLLPLIF IL+HI SSSKQPP PPGP PWP+LGNLLQIGQNAHISMTQFS
Sbjct: 1   MEIASFSFLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFS 60

Query: 61  HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSP 120
           H YGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FSP
Sbjct: 61  HFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSP 120

Query: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVF 180
           ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMN+MVEFLQSKQGSVVEIKE VFITVF
Sbjct: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVF 180

Query: 181 NTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAI 240
           NTLSNFIFSRDLVGFVGDGFNGI+G FKN+ID  LIPNLADFYPLLR FDLQGLRKKAAI
Sbjct: 181 NTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAI 240

Query: 241 YKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTT 300
           YKNQ+DSSWEILIKERRTIHSR V   SDFLDVLI+SGFTDEQI+YLIVEVLSAGTDTTT
Sbjct: 241 YKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTT 300

Query: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPF 360
           TTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAPF
Sbjct: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPF 360

Query: 361 LLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDY 420
           LLPRYAPEACELMGYSVPK TMI VNVWGIGRDPSIWEDP SFKPERFD  DADLKGHDY
Sbjct: 361 LLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDY 420

Query: 421 RFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEE 480
           RFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEW LPNDEDASTLDL GRLGVTLQKEE
Sbjct: 421 RFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEE 480

Query: 481 PLKLLPKRR 489
           PLKLL K+R
Sbjct: 481 PLKLLSKKR 489

[Close]
BLAST of CmoCh15G014820 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.7e-210
Identity = 366/488 (75.00%), Postives = 418/488 (85.66%), Query Frame = 0 

Query: 7   SFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLI 66
           S LLLLP IF ILKHI S S + P PPGP PWP+LGNLLQIGQNAHIS+TQF++IYGPLI
Sbjct: 12  SLLLLLP-IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 71

Query: 67  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWK 126
           SLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQM PDRALHD  SLVFSPECGD+WK
Sbjct: 72  SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 131

Query: 127 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFI 186
           NLRS+C+  LFTA+AIESQA LR KKM E+V+FL+SKQGSVVE+K+FVF +VFNTLSN I
Sbjct: 132 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 191

Query: 187 FSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDS 246
           FS DL+ FVGDGF GI+G FK ++D  L PNLADFYPLLR FDLQGL+ KA IYKN++DS
Sbjct: 192 FSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 251

Query: 247 SWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAM 306
            W ILIKERR IH +    ++DFLDVLIQSGF D+QIN LI+E+LSAGTDTTTTTVEWAM
Sbjct: 252 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 311

Query: 307 AELLKNRDIMNRVREETKKAINGD-AIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 366
           +E+LK++DI+N+VREE KK    +  IDES +SEL YLW+CVKETLRLHPPAPFLLPR A
Sbjct: 312 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 371

Query: 367 PEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDV-C---DADLKGHDYRF 426
           P  CE+MGYS+PK +M+ VNVWGIGRDPS+WED Q+F P+RFDV C   + D KG+DYR+
Sbjct: 372 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 431

Query: 427 LPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPL 486
           LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPNDED S LDL GRLGVTLQK E L
Sbjct: 432 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 491

Query: 487 KLLPKRRI 490
           KL+PKRR+
Sbjct: 492 KLIPKRRV 498

[Close]
BLAST of CmoCh15G014820 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 2.9e-210
Identity = 366/488 (75.00%), Postives = 418/488 (85.66%), Query Frame = 0 

Query: 7   SFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPLI 66
           S LLLLP IF ILKHI S S + P PPGP PWP+LGNLLQIGQNAHIS+TQF++IYGPLI
Sbjct: 12  SLLLLLP-IFIILKHIKSPSSKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 71

Query: 67  SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWK 126
           SLKLGAQRL+VASSPAAA A+LKTQDRLLSARYIFQM PDRALHD  SLVFSPECGD+WK
Sbjct: 72  SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 131

Query: 127 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNFI 186
           NLRS+C+  LFTA+AIESQA LR KKM E+V+FL+SKQGSVVE+K+FVF +VFNTLSN I
Sbjct: 132 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 191

Query: 187 FSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDS 246
           FS DL+ FVGDGF GI+G FK ++D  L PNLADFYPLLR FDLQGL+ KA IYKN++DS
Sbjct: 192 FSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 251

Query: 247 SWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWAM 306
            W ILIKERR IH +    ++DFLDVLIQSGF D+QIN LI+E+LSAGTDTTTTTVEWAM
Sbjct: 252 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 311

Query: 307 AELLKNRDIMNRVREETKKAINGD-AIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYA 366
           +E+LK++DI+N+VREE KK    +  IDES +SEL YLW+CVKETLRLHPPAPFLLPR A
Sbjct: 312 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 371

Query: 367 PEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDV-C---DADLKGHDYRF 426
           P  CE+MGYS+PK +M+ VNVWGIGRDPS+WED Q+F P+RFDV C   + D KG+DYR+
Sbjct: 372 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 431

Query: 427 LPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPL 486
           LPFGGGRR+CPGLPMA VQVPLILATLIHNFEW LPNDED S LDL GRLGVTLQK E L
Sbjct: 432 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 491

Query: 487 KLLPKRRI 490
           KL+PKRR+
Sbjct: 492 KLIPKRRV 498

[Close]
BLAST of CmoCh15G014820 vs. NCBI nr
Match: XP_022929108.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 987.3 bits (2551), Expect = 4.9e-284
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0 

Query: 1   MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH 60
           MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH
Sbjct: 1   MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH 60

Query: 61  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 120
           IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE
Sbjct: 61  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 120

Query: 121 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 180
           CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN
Sbjct: 121 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 180

Query: 181 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 240
           TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY
Sbjct: 181 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 240

Query: 241 KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT 300
           KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT
Sbjct: 241 KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT 300

Query: 301 TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL 360
           TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL
Sbjct: 301 TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL 360

Query: 361 LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR 420
           LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR
Sbjct: 361 LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR 420

Query: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP 480
           FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP
Sbjct: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP 480

Query: 481 LKLLPKRRI 490
           LKLLPKRRI
Sbjct: 481 LKLLPKRRI 489

[Close]
BLAST of CmoCh15G014820 vs. NCBI nr
Match: KAG7017133.1 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 937.9 bits (2423), Expect = 3.4e-269
Identity = 469/490 (95.71%), Postives = 476/490 (97.14%), Query Frame = 0 

Query: 1   MEIASFSF--LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQF 60
           MEIASFSF  LLLLPLI AILKHISSSSKQPP PPGPKPWPMLGNLLQIGQNAHISMTQF
Sbjct: 1   MEIASFSFLLLLLLPLIVAILKHISSSSKQPPLPPGPKPWPMLGNLLQIGQNAHISMTQF 60

Query: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120
           SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS
Sbjct: 61  SHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFS 120

Query: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180
           PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV
Sbjct: 121 PECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITV 180

Query: 181 FNTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAA 240
           FNTLSNFIFSRDLVGFVGDGFNGI+GHFKNVID ALIPNLADFYPLLRCFDLQGLRKKAA
Sbjct: 181 FNTLSNFIFSRDLVGFVGDGFNGIKGHFKNVIDLALIPNLADFYPLLRCFDLQGLRKKAA 240

Query: 241 IYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTT 300
           IYKNQIDSSWEILIKERRTIHSRGV   +DFLDVLIQSG+T+EQINYLIVEVLSAGTD  
Sbjct: 241 IYKNQIDSSWEILIKERRTIHSRGVSAATDFLDVLIQSGYTNEQINYLIVEVLSAGTD-- 300

Query: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAP 360
           TTTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAP
Sbjct: 301 TTTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAP 360

Query: 361 FLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHD 420
           FLLPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD CDADLKGHD
Sbjct: 361 FLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHD 420

Query: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKE 480
           YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDE+ASTLDL GRLG+TLQKE
Sbjct: 421 YRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDENASTLDLNGRLGITLQKE 480

Query: 481 EPLKLLPKRR 489
           EPLKLLPK+R
Sbjct: 481 EPLKLLPKKR 488

[Close]
BLAST of CmoCh15G014820 vs. NCBI nr
Match: XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])

HSP 1 Score: 929.1 bits (2400), Expect = 1.6e-266
Identity = 462/489 (94.48%), Postives = 471/489 (96.32%), Query Frame = 0 

Query: 1   MEIASFSF-LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFS 60
           MEIASFSF LLLLPLIF IL+HISSSSKQPP PPGPKPWP+LGNLLQIGQNAHISMTQFS
Sbjct: 1   MEIASFSFLLLLLPLIFIILRHISSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFS 60

Query: 61  HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSP 120
           HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FSP
Sbjct: 61  HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSP 120

Query: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVF 180
           ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKE VFITVF
Sbjct: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVF 180

Query: 181 NTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAI 240
           NTLSNFIFSRDLVGFVGDGFNGI+G FKN+ID  LIPNLADFYPLLRCFDLQGLRKKAAI
Sbjct: 181 NTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAI 240

Query: 241 YKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTT 300
           YKNQ+DSSWEILIKERRTIHS+GV   SDFLDVLIQSGFTDEQI+YLIVEVLSAGTDTTT
Sbjct: 241 YKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTT 300

Query: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPF 360
           TTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAPF
Sbjct: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPF 360

Query: 361 LLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDY 420
           LLPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD CDADLKGHDY
Sbjct: 361 LLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCDADLKGHDY 420

Query: 421 RFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEE 480
           RFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDL GRLGVTLQKEE
Sbjct: 421 RFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEE 480

Query: 481 PLKLLPKRR 489
           PLKLL K R
Sbjct: 481 PLKLLSKNR 489

[Close]
BLAST of CmoCh15G014820 vs. NCBI nr
Match: XP_023551257.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 907.1 bits (2343), Expect = 6.4e-260
Identity = 448/488 (91.80%), Postives = 465/488 (95.29%), Query Frame = 0 

Query: 1   MEIASFSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSH 60
           MEIASFSFLLLLPLIF +LKHISSSSKQPP PPGP PWP+LGNLLQIGQNAHISMTQFSH
Sbjct: 1   MEIASFSFLLLLPLIFTVLKHISSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSH 60

Query: 61  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPE 120
           IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYI QM PDRALHDNCSL+FSPE
Sbjct: 61  IYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYILQMTPDRALHDNCSLLFSPE 120

Query: 121 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFN 180
           CGDRWKNLRSICRAKLFTARAIESQASLRRKKM +MVEFLQSKQGSVVEIKEFVFITVFN
Sbjct: 121 CGDRWKNLRSICRAKLFTARAIESQASLRRKKMKDMVEFLQSKQGSVVEIKEFVFITVFN 180

Query: 181 TLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIY 240
           TLSN IFSRDLV FVGDGFNGI+G FKN+ID  LIPNLADFYPLLR FDLQGLRKKAAIY
Sbjct: 181 TLSNLIFSRDLVDFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRSFDLQGLRKKAAIY 240

Query: 241 KNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTT 300
           KNQIDSS EILIKERRTIHSRGV   SDFLD+LI+SGFT+EQI+YLI+EVL AGTDTTTT
Sbjct: 241 KNQIDSSSEILIKERRTIHSRGVSAASDFLDILIESGFTNEQISYLILEVLLAGTDTTTT 300

Query: 301 TVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPFL 360
           TVEWAMAEL+KNRDIMNRVREETKKA+NG AIDESKLSELHYLWKCVKET+RLHPPAPFL
Sbjct: 301 TVEWAMAELMKNRDIMNRVREETKKALNGGAIDESKLSELHYLWKCVKETVRLHPPAPFL 360

Query: 361 LPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDYR 420
           LPRYAPEACELMGYSVPKHTMI VNVWGIGRDPSIWEDPQSFKPERFD  DADLKGHDYR
Sbjct: 361 LPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDSSDADLKGHDYR 420

Query: 421 FLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEP 480
           FLPFGGGRR CPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDL GRLG+TLQKEEP
Sbjct: 421 FLPFGGGRRTCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGITLQKEEP 480

Query: 481 LKLLPKRR 489
           LKL+PK+R
Sbjct: 481 LKLVPKKR 488

[Close]
BLAST of CmoCh15G014820 vs. NCBI nr
Match: XP_022970204.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima])

HSP 1 Score: 904.0 bits (2335), Expect = 5.4e-259
Identity = 452/489 (92.43%), Postives = 463/489 (94.68%), Query Frame = 0 

Query: 1   MEIASFSF-LLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFS 60
           MEIASFSF LLLLPLIF IL+HI SSSKQPP PPGP PWP+LGNLLQIGQNAHISMTQFS
Sbjct: 1   MEIASFSFLLLLLPLIFTILRHICSSSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFS 60

Query: 61  HIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSP 120
           H YGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQM PDR LHDNCSL+FSP
Sbjct: 61  HFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSP 120

Query: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVF 180
           ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMN+MVEFLQSKQGSVVEIKE VFITVF
Sbjct: 121 ECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVF 180

Query: 181 NTLSNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAI 240
           NTLSNFIFSRDLVGFVGDGFNGI+G FKN+ID  LIPNLADFYPLLR FDLQGLRKKAAI
Sbjct: 181 NTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAI 240

Query: 241 YKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTT 300
           YKNQ+DSSWEILIKERRTIHSR V   SDFLDVLI+SGFTDEQI+YLIVEVLSAGTDTTT
Sbjct: 241 YKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTT 300

Query: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGDAIDESKLSELHYLWKCVKETLRLHPPAPF 360
           TTVEWAMAELLKNRDIMNRVREETKKAING AIDESKLSELHYLWKCVKETLRLHPPAPF
Sbjct: 301 TTVEWAMAELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPF 360

Query: 361 LLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDVCDADLKGHDY 420
           LLPRYAPEACELMGYSVPK TMI VNVWGIGRDPSIWEDP SFKPERFD  DADLKGHDY
Sbjct: 361 LLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFDADLKGHDY 420

Query: 421 RFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEE 480
           RFLPFGGGRR+CPGLPMAAVQVPLILATLIHNFEW LPNDEDASTLDL GRLGVTLQKEE
Sbjct: 421 RFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEE 480

Query: 481 PLKLLPKRR 489
           PLKLL K+R
Sbjct: 481 PLKLLSKKR 489

[Close]
BLAST of CmoCh15G014820 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 349.7 bits (896), Expect = 3.7e-96
Identity = 203/501 (40.52%), Postives = 293/501 (58.48%), Query Frame = 0 

Query: 6   FSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 65
           F F+L   +IF       SS K  P PPGP   P++GN+  +G+N H S    S  YGP+
Sbjct: 13  FCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPI 72

Query: 66  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRAL-HDNCSLVFSPECGDR 125
           +SLK G+   VV +SP AA  +L+T D++LS+R     I  R++ HD  S+V+ P    R
Sbjct: 73  MSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSI--RSINHDKVSVVWLPPSSSR 132

Query: 126 WKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSVVEIKEFVFITVFNTL 185
           W+ LR +   +LF+ + IE+  +LR  K+ E+V F+   S++   V+I    FIT  N +
Sbjct: 133 WRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNII 192

Query: 186 SNFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRK--KAA-- 245
           SN +FS DL  +  +     +     V++    P+ A+F+P L   DLQG RK  KA   
Sbjct: 193 SNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSE 252

Query: 246 ----IYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDE------QINYLIV 305
               +++  ID+  ++  K  R  +S+ V    DF+DVL+     DE       I +L++
Sbjct: 253 RLFKVFRGFIDA--KLAEKSLRDTNSKDVR-ERDFVDVLLDLTEGDEAELNTNDIVHLLL 312

Query: 306 EVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAI-NGDAIDESKLSELHYLWKCV 365
           ++  AGTDT ++TVEWAMAELL+N + M + + E    I     ++ES +S L YL   V
Sbjct: 313 DLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAVV 372

Query: 366 KETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERF 425
           KET RLHP AP L+PR A    E++G+ VPK T + VNVW IGRDP++WE+   FKPERF
Sbjct: 373 KETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPERF 432

Query: 426 DVCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDL 485
              D DL+G DY   PFG GRR+CPGLP+A   VPL+LA+L+++F+W LPN   +  LD+
Sbjct: 433 LGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDLDM 492

Query: 486 KGRLGVTLQKEEPLKLLPKRR 489
               G+TL K  PL  +P ++
Sbjct: 493 DETFGLTLHKTNPLHAVPVKK 508

[Close]
BLAST of CmoCh15G014820 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 335.5 bits (859), Expect = 7.2e-92
Identity = 190/501 (37.92%), Postives = 293/501 (58.48%), Query Frame = 0 

Query: 6   FSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 65
           F F+L   LIF   +    S      PPGP   P++GN+  +G++ H S  + S  YGP+
Sbjct: 13  FCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPV 72

Query: 66  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRAL-HDNCSLVFSPECGDR 125
           +SLKLG+   VV +SP AA  +L+T D++LSAR     +  R++ H + SLV+ P    R
Sbjct: 73  MSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAV--RSINHQDASLVWLPSSSAR 132

Query: 126 WKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSVVEIKEFVFITVFNTL 185
           W+ LR +   +L + + IE+  +LR  K+ E+V F+   S +   V+I    FIT  N +
Sbjct: 133 WRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFITTLNII 192

Query: 186 SNFIFSRDLVGF-VGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQG--------L 245
           SN +FS DL  +      NG++    +V+D A  P+ A+++P LR  DLQG         
Sbjct: 193 SNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCT 252

Query: 246 RKKAAIYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLI-----QSGFTDEQINYLIV 305
            +   +++  ID+     I E+ + ++   +  +DF+D L+     +S  +   I +L++
Sbjct: 253 ERLVRVFRGFIDAK----IAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEHLLL 312

Query: 306 EVLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAINGDAI-DESKLSELHYLWKCV 365
           ++ +AGTDT+++T+EWAM ELLKN   M + + E    I  + I +ES +S+L YL   V
Sbjct: 313 DMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVV 372

Query: 366 KETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERF 425
           KET RLH P P L+PR A    E++G+ V K T ++VNVW IGRDPS+W++P  F+PERF
Sbjct: 373 KETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERF 432

Query: 426 DVCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDL 485
              D D++G DY   PFG GRR+CPG+P+A   V L+LA+L+++F+W LP    +  LD+
Sbjct: 433 LGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDM 492

Query: 486 KGRLGVTLQKEEPLKLLPKRR 489
               G+TL K  PL  +P ++
Sbjct: 493 DETFGLTLHKTNPLHAVPVKK 507

[Close]
BLAST of CmoCh15G014820 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 334.7 bits (857), Expect = 1.2e-91
Identity = 181/481 (37.63%), Postives = 283/481 (58.84%), Query Frame = 0 

Query: 32  PPGPKPWPMLGNLLQI-GQNAHISMTQFSHIYGPLISLKLGAQRLVVASSPAAAAAILKT 91
           PPGP PWP++GN+ Q+ G   H S+T+ S  +GP+++L++G+   VV SS   A  I K 
Sbjct: 37  PPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFKK 96

Query: 92  QDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRWKNLRSICRAKLFTARAIESQASLRR 151
            D  L+ R I++ +      D    + + + G  W+ LR +C  + F  R +++ + +R 
Sbjct: 97  HDAALAGRKIYEAMKGGKSSDGS--LITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVRS 156

Query: 152 KKMNEMVEFLQ--SKQGS-VVEIKEFVFITVFNTLSNFIFSRDLVGFVGDGFNGIE--GH 211
           + +++M+ F++   + G+  +++  + F+  FN + N +FSRDL+    D   G E   H
Sbjct: 157 RCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFYH 216

Query: 212 FKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQIDSSWEI---LIKERRTIHSR- 271
              V+++A  PN+ADF+PLLR  D QG+R+K   +   ++ ++EI    I+ER  +  R 
Sbjct: 217 TGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFH---VEKAFEIAGEFIRERTEVRERE 276

Query: 272 -GVLVTSDFLDVLIQ-----------SGFTDEQINYLIVEVLSAGTDTTTTTVEWAMAEL 331
                T D+LDVL++           S F+   IN ++ E+ +AGTDTTT+T+EWA+AEL
Sbjct: 277 KSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAEL 336

Query: 332 LKNRDIMNRVREETKKAI--NGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAPE 391
           L N   + +++ E +     +   + E  L  L YL   + ETLRLHPP PFL+P  A  
Sbjct: 337 LHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMS 396

Query: 392 ACELMG-YSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERF--DVCDADLKGHDYRFLPF 451
            C +   Y++PK T ++VNVW IGRDP  W DP  FKPERF  D    D KG DY FLPF
Sbjct: 397 TCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDARDFKGQDYEFLPF 456

Query: 452 GGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPLKLL 486
           G GRR+CP LP+A+  +PL + +++ +F+W L N  +A  +D+  R+G+TL+K  PL+ +
Sbjct: 457 GSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAI 510

[Close]
BLAST of CmoCh15G014820 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 333.2 bits (853), Expect = 3.6e-91
Identity = 185/500 (37.00%), Postives = 284/500 (56.80%), Query Frame = 0 

Query: 6   FSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 65
           F F+    LI    +   SS +    PPGP   P++GN+ Q+G+N H S    + IYGP+
Sbjct: 13  FCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPI 72

Query: 66  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 125
           +SLK G    VV +SP AA  +L+T D++LS R     I     H+  S+++ P    RW
Sbjct: 73  MSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEVSVIWLPPSSARW 132

Query: 126 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFL--QSKQGSVVEIKEFVFITVFNTLS 185
           + LR +    +F+ +  E+  +LR KK+ E+V F+   S++   V+I    + TV N +S
Sbjct: 133 RMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIIS 192

Query: 186 NFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKA------ 245
           N +FS DL  +     N  +      ++ A  P+ A+++P +   DLQG RK        
Sbjct: 193 NILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTER 252

Query: 246 --AIYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLI------QSGFTDEQINYLIVE 305
              +++  +D+     I E+   +    +   DFLD L+      ++   +  I +L+++
Sbjct: 253 LFRVFRGFMDAK----IAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLD 312

Query: 306 VLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAI-NGDAIDESKLSELHYLWKCVK 365
           + +AGTDT+++T+EWAMAELL+N   M + + E  + +     + ES +S L YL   VK
Sbjct: 313 MFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVK 372

Query: 366 ETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFD 425
           ET RLHP AP L+PR A    E++G+ VPK T ++VNVW IGRDPS+WE+P  F+PERF 
Sbjct: 373 ETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFM 432

Query: 426 VCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLK 485
             D D+KG DY   PFGGGRR+CPGLP+A   V L+LA+L+++F+W LPN   +  LD+ 
Sbjct: 433 GKDIDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMD 492

Query: 486 GRLGVTLQKEEPLKLLPKRR 489
              G+TL +   L  +P ++
Sbjct: 493 ETFGITLHRTNTLYAIPVKK 507

[Close]
BLAST of CmoCh15G014820 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 329.3 bits (843), Expect = 5.1e-90
Identity = 194/501 (38.72%), Postives = 285/501 (56.89%), Query Frame = 0 

Query: 6   FSFLLLLPLIFAILKHISSSSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 65
           F F+L   L F   +   S  +    PPGP   P++GN+  +G+N H S T  S  YGP+
Sbjct: 13  FCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPV 72

Query: 66  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 125
           +SLKLG    VV +S  A   +LKT D++LS RYI +       H   S+ +      R+
Sbjct: 73  MSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNN-HHEFSVGWIHPSSSRF 132

Query: 126 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQS--KQGSVVEIKEFVFITVFNTLS 185
           + LR +   +LF+ + I++  +LR KK+ E+V FL    ++   V+I    F+T  N +S
Sbjct: 133 RMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNIIS 192

Query: 186 NFIFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKK------- 245
           N +FS +L  +     +  +       +    P+LA+F+P +R  DLQG  KK       
Sbjct: 193 NILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSGR 252

Query: 246 -AAIYKNQIDSSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDE------QINYLIVE 305
              +++   D+   I+ K  R++     + + DFLDVLI     DE      +I +L+++
Sbjct: 253 LLQVFREFYDA--RIVEKSSRSVEKD--VSSKDFLDVLIDLQQGDETEINIDEIEHLLLD 312

Query: 306 VLSAGTDTTTTTVEWAMAELLKNRDIMNRVREETKKAI--NGDAIDESKLSELHYLWKCV 365
           +  AGTDT ++TVEWAMAELL N   M +V++E    I  NGD   ES +S+L YL   V
Sbjct: 313 MFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGD-FQESDISKLPYLKAVV 372

Query: 366 KETLRLHPPAPFLLPRYAPEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERF 425
           KET RLHP APFLL R A    E++G++V K + ++VNVW IGRDP +WE+P  F+PERF
Sbjct: 373 KETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTHFEPERF 432

Query: 426 DVCDADLKGHDYRFLPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDL 485
              + D+KG DY   PFG GRR+CPGLP+A   V L+LA+L++ FEW LPN   +  LD+
Sbjct: 433 LGKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGVGSEDLDM 492

Query: 486 KGRLGVTLQKEEPLKLLPKRR 489
           +   G+T+ K  PL  +P ++
Sbjct: 493 EETFGLTVHKTNPLLAVPLKK 507

The following BLAST results are available for this feature:
BLAST of CmoCh15G014820 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9FXW43.0e-11946.52Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... [more]
O649001.4e-11643.70(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... [more]
O648991.8e-11643.89(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... [more]
Q9SP062.5e-11343.66(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... [more]
A8CDR53.0e-10340.89Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1[more]
BLAST of CmoCh15G014820 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1ER522.4e-284100.00probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A6J1ELA67.6e-26794.48probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... [more]
A0A6J1I4V72.6e-25992.43probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... [more]
A0A5A7UIU31.7e-21075.00Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... [more]
A0A1S3CT142.9e-21075.00probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... [more]
BLAST of CmoCh15G014820 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022929108.14.9e-284100.00probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata][more]
KAG7017133.13.4e-26995.71(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2, partial [Cucurbita argyrosperma... [more]
XP_022928791.11.6e-26694.48probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata][more]
XP_023551257.16.4e-26091.80probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita pepo subsp. ... [more]
XP_022970204.15.4e-25992.43probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita maxima][more]
BLAST of CmoCh15G014820 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G45570.13.7e-9640.52cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT2G45560.17.2e-9237.92cytochrome P450, family 76, subfamily C, polypeptide 1 [more]
AT3G52970.11.2e-9137.63cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
AT2G45550.13.6e-9137.00cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT1G33720.15.1e-9038.72cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 431..442
score: 28.4
coord: 345..356
score: 39.99
coord: 293..310
score: 41.21
coord: 422..431
score: 59.57
IPR001128Cytochrome P450PFAMPF00067p450coord: 32..476
e-value: 1.8E-96
score: 323.7
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 344..362
score: 32.62
coord: 302..328
score: 25.88
coord: 421..431
score: 57.4
coord: 59..78
score: 34.03
coord: 282..299
score: 27.98
coord: 385..409
score: 36.76
coord: 431..454
score: 31.32
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 17..487
e-value: 4.4E-111
score: 373.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 32..488
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 14..467
e-value: 3.1E-16
score: 57.2
NoneNo IPR availablePANTHERPTHR47956:SF23(S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 1-LIKEcoord: 5..486
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 5..486
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 424..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G014820.1CmoCh15G014820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen