CmoCh15G010080 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G010080
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr15: 5577017 .. 5579857 (-)
RNA-Seq ExpressionCmoCh15G010080
SyntenyCmoCh15G010080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGGAACTCCATCCCTTAATAAACAGTCATTACCATGGAAATGCTGCATTGTTTTCTTCTCTTTTCATTCCTAGGGATCACCCATGCCCAAAGCTTCCACAATTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAAACCGAATCACAACGTACCCACCACCGACGTCCATCAATCTTATCACATCACTCGCTTTCGAAACTCGGCCATTCGAGCCACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTTCCATGGATTTAGCTTCCTTTCACGATGACGACAGAGTAAAATCAGCTTTAATCGACTGTCTGGAACTCTATGAAGATTCAATCACCGAGCTCAATCGCTCGGTGATGAACTCTATGAAGGCCAATTCAATTGGCCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGTCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATAATATCATTTTTCCACATCAAATTTTGAGTAACTTCTCGAAATTGATCAGCAATTCTCTTGCCATCACCAAGGCCGCTGCCGTGACAGCTACTACTTCTTCCACCCCTGGTAGCCAGCGGCTACTAGCTGGGTCGTTTCCCAAGTGGGTTTCTGGTGATGATAGGATGCTCCTTCGGGACACGACCGAGAAGCGCACGGATATGGTGGTGGCCCAAGATGGGAGCGGCAATTTCAGAACGATATCGGAGGCGGTGGCCGCCGCGGCGGAGGTTAGGAAGGGAAGTGAAAGAAGGTTTGTTATATATGTAAAAGGTGGGATTTACAGAGAGAATGTTGTGATTAAGAAAACGTTGAAGAATGTTATGATGATTGGAGATGGGATGGGCGAGACGATCGTTACGGCCATGAAAAACGTCAAAGATGGATCAACCACTTTCCGATCAGCCACTTTTGGTTCGTAAATCCTTATGTTTTAATCCTATTTTTATGTTAATTTTGCTTCCATGCATGCATGCAAATGGATCTACAAGTTATAGTTTCCAATAGAATTGAATTTAAAAGCTTCAATAGGCTTTGAGTTTACTATCAATTTGTTTTCTAAGAGTTTTAGGACCTTTAATTTGATCAAATACAATCAATTAATAACTTATTAAACATGTTCATAGCGTAAAAGTTAAAAGTTATGTTAAAAATCATTTTAAATTTTAGAAACCAAATTTGTAATTGAAAATATTTACGAATAAATGAAGCCAAAAATAAAAAATTGCAACTCATGGACCAAATTGATTGAATTACCGACCAAGTCATAACTCATCGGAAAAAATGTTGGCGGCAATTTCAGCGGTGGTCGGCGAAGGGTTCATCGCCAAAGACATGACGTTTGAGAACAGGGCAGGAGCAGAGAAGCACCAAGCTGTTGCCCTCCGAACTAACGCCGATCGCTCAATATTCTATCGATGCAGCTTCAAAGGCTACCAAGACACTCTATACGCCCACTCCAACCGCCATTTCTTCCGTGAATGCCACATTTACGGAACCATCGACTTCATTTTCGGCGACGGCGCCTCTGTATTTCAAAATTGCAACATCTTCGTCAGAAAGCCGATGGAAAACCAGAAAAACACGGTGACGGCGCAGGGGAGGTCCGACCCGAATGAAAACACGGGGTTTGTGATACATAATTCCGTTGTCAGAGCGGCACCGGACCTGAAGGCGGTGGAGGGTGTGTACAAAACCTATTTGGGTAGGCCGTGGAAGGCGTATTCGAGGACGGTGGTAATGAGGAGCTTTTTGGATGGGCTAATTGAACCGGCGGGGTGGCTTCCTTGGGCCGGAGATTTCGGTCTGAAGACGGTTTATTATGGGGAGTTTAAGAACAGCGGTGGCGGGGCAAGTACAATGAGGAGGGTGCAGTGGCCGGGGTACCATGTCATGACGACTGCGGCGGAGGCCGAGAGATTTACAGTGGAAAGTTTCCTGGAAGGAGGAGGTTGGATTCCGGGCACCGGAGTGCCATTTGCAAATGGGGTATGATTATGAAATAGAAGTTGATAGAAAAGATATTTGGCTATACATCGGTCAATGGTTGTTGCTGCTGTACATTTTGAAAGTGGTGTACCATAAAAGTTAATGGTGGTATGGTGGTAAGAAATAAGGTCCATTTGGAAGAGAAAAATGGGGTGGGGGGAAAGTTGGGCAGTGCAAAAAGATGTAAGAAAAATGTTGTGCAATTTGTGTTAGGATTTGTTTGGGGACAAAAATAGTTGGGTATATAGCTTATTATAGTTGGAAAACTTTTATTTCTTTTTTCCCTTCTTTTCATCCTATTATGAAAGACTAAATTAATGTAATAAATAGTTATCAACCAGGATTAGATCCTCATTTTGGTTTCCTATTTTTCCTTCTTAGTTCTTAAAGAAGCACAAGCAAAGTGAGATAAAAAGTATATATTTTGTTCAGAAAGCATAAGAAATAAAACGTTAATCCAATCAAACCCTAAACTGATGAACAGACCAACAATATTAATAGGGTAAGCTTATACCAAAAGTGGGGAAAAAACCACATTAGTTCATTCAAATATATATTATATATTCTATTACTTTGTCGAGCTTCATAAGAATGAAGCATCAAAAGATTATTAAGACAATCAAAATCCAAAATTTATGTAGGCTAAAGTTTTCCCGCCAAACAAACACTCTGGAAGCACTTACAGTGATGTTTTAAGAGATCTTGCGCCACCGCAAGCTAGCAACAATTATTTCTTCAAAATCATGGGAAATTCTTGCTTAAGTAATGACGGTAGGAGCAGATCGGCCGGTAAATTCTTGCATTTTTGA

mRNA sequence

AAAAGGAACTCCATCCCTTAATAAACAGTCATTACCATGGAAATGCTGCATTGTTTTCTTCTCTTTTCATTCCTAGGGATCACCCATGCCCAAAGCTTCCACAATTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAAACCGAATCACAACGTACCCACCACCGACGTCCATCAATCTTATCACATCACTCGCTTTCGAAACTCGGCCATTCGAGCCACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTTCCATGGATTTAGCTTCCTTTCACGATGACGACAGAGTAAAATCAGCTTTAATCGACTGTCTGGAACTCTATGAAGATTCAATCACCGAGCTCAATCGCTCGGTGATGAACTCTATGAAGGCCAATTCAATTGGCCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGTCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATAATATCATTTTTCCACATCAAATTTTGAGTAACTTCTCGAAATTGATCAGCAATTCTCTTGCCATCACCAAGGCCGCTGCCGTGACAGCTACTACTTCTTCCACCCCTGGTAGCCAGCGGCTACTAGCTGGGTCGTTTCCCAAGTGGGTTTCTGGTGATGATAGGATGCTCCTTCGGGACACGACCGAGAAGCGCACGGATATGGTGGTGGCCCAAGATGGGAGCGGCAATTTCAGAACGATATCGGAGGCGGTGGCCGCCGCGGCGGAGGTTAGGAAGGGAAGTGAAAGAAGGTTTGTTATATATGTAAAAGGTGGGATTTACAGAGAGAATGTTGTGATTAAGAAAACGTTGAAGAATGTTATGATGATTGGAGATGGGATGGGCGAGACGATCGTTACGGCCATGAAAAACGTCAAAGATGGATCAACCACTTTCCGATCAGCCACTTTTGCGGTGGTCGGCGAAGGGTTCATCGCCAAAGACATGACGTTTGAGAACAGGGCAGGAGCAGAGAAGCACCAAGCTGTTGCCCTCCGAACTAACGCCGATCGCTCAATATTCTATCGATGCAGCTTCAAAGGCTACCAAGACACTCTATACGCCCACTCCAACCGCCATTTCTTCCGTGAATGCCACATTTACGGAACCATCGACTTCATTTTCGGCGACGGCGCCTCTGTATTTCAAAATTGCAACATCTTCGTCAGAAAGCCGATGGAAAACCAGAAAAACACGGTGACGGCGCAGGGGAGGTCCGACCCGAATGAAAACACGGGGTTTGTGATACATAATTCCGTTGTCAGAGCGGCACCGGACCTGAAGGCGGTGGAGGGTGTGTACAAAACCTATTTGGGTAGGCCGTGGAAGGCGTATTCGAGGACGGTGGTAATGAGGAGCTTTTTGGATGGGCTAATTGAACCGGCGGGGTGGCTTCCTTGGGCCGGAGATTTCGGTCTGAAGACGGTTTATTATGGGGAGTTTAAGAACAGCGGTGGCGGGGCAAGTACAATGAGGAGGGTGCAGTGGCCGGGGTACCATGTCATGACGACTGCGGCGGAGGCCGAGAGATTTACAGTGGAAAGTTTCCTGGAAGGAGGAGGTTGGATTCCGGGCACCGGAGTGCCATTTGCAAATGGGGCTAAAGTTTTCCCGCCAAACAAACACTCTGGAAGCACTTACAGTGATGTTTTAAGAGATCTTGCGCCACCGCAAGCTAGCAACAATTATTTCTTCAAAATCATGGGAAATTCTTGCTTAAGTAATGACGGTAGGAGCAGATCGGCCGGTAAATTCTTGCATTTTTGA

Coding sequence (CDS)

ATGGAAATGCTGCATTGTTTTCTTCTCTTTTCATTCCTAGGGATCACCCATGCCCAAAGCTTCCACAATTTCATGTCTTGCTCCCAAACCCCATATCCAGACCTCTGCTTCCATTTCATCAAACCGAATCACAACGTACCCACCACCGACGTCCATCAATCTTATCACATCACTCGCTTTCGAAACTCGGCCATTCGAGCCACTTTGAACCAAGCCATGGTGGCCCATCACCACCTTCTCTCTTCCATGGATTTAGCTTCCTTTCACGATGACGACAGAGTAAAATCAGCTTTAATCGACTGTCTGGAACTCTATGAAGATTCAATCACCGAGCTCAATCGCTCGGTGATGAACTCTATGAAGGCCAATTCAATTGGCCACTTGACATTGCTAAGTGCTTCATTAGCGAATCATCAAACATGTCGAGATGGGTTTCGAGATTTTGGATTTTCTTCTCATTATTTGAATAATATCATTTTTCCACATCAAATTTTGAGTAACTTCTCGAAATTGATCAGCAATTCTCTTGCCATCACCAAGGCCGCTGCCGTGACAGCTACTACTTCTTCCACCCCTGGTAGCCAGCGGCTACTAGCTGGGTCGTTTCCCAAGTGGGTTTCTGGTGATGATAGGATGCTCCTTCGGGACACGACCGAGAAGCGCACGGATATGGTGGTGGCCCAAGATGGGAGCGGCAATTTCAGAACGATATCGGAGGCGGTGGCCGCCGCGGCGGAGGTTAGGAAGGGAAGTGAAAGAAGGTTTGTTATATATGTAAAAGGTGGGATTTACAGAGAGAATGTTGTGATTAAGAAAACGTTGAAGAATGTTATGATGATTGGAGATGGGATGGGCGAGACGATCGTTACGGCCATGAAAAACGTCAAAGATGGATCAACCACTTTCCGATCAGCCACTTTTGCGGTGGTCGGCGAAGGGTTCATCGCCAAAGACATGACGTTTGAGAACAGGGCAGGAGCAGAGAAGCACCAAGCTGTTGCCCTCCGAACTAACGCCGATCGCTCAATATTCTATCGATGCAGCTTCAAAGGCTACCAAGACACTCTATACGCCCACTCCAACCGCCATTTCTTCCGTGAATGCCACATTTACGGAACCATCGACTTCATTTTCGGCGACGGCGCCTCTGTATTTCAAAATTGCAACATCTTCGTCAGAAAGCCGATGGAAAACCAGAAAAACACGGTGACGGCGCAGGGGAGGTCCGACCCGAATGAAAACACGGGGTTTGTGATACATAATTCCGTTGTCAGAGCGGCACCGGACCTGAAGGCGGTGGAGGGTGTGTACAAAACCTATTTGGGTAGGCCGTGGAAGGCGTATTCGAGGACGGTGGTAATGAGGAGCTTTTTGGATGGGCTAATTGAACCGGCGGGGTGGCTTCCTTGGGCCGGAGATTTCGGTCTGAAGACGGTTTATTATGGGGAGTTTAAGAACAGCGGTGGCGGGGCAAGTACAATGAGGAGGGTGCAGTGGCCGGGGTACCATGTCATGACGACTGCGGCGGAGGCCGAGAGATTTACAGTGGAAAGTTTCCTGGAAGGAGGAGGTTGGATTCCGGGCACCGGAGTGCCATTTGCAAATGGGGCTAAAGTTTTCCCGCCAAACAAACACTCTGGAAGCACTTACAGTGATGTTTTAAGAGATCTTGCGCCACCGCAAGCTAGCAACAATTATTTCTTCAAAATCATGGGAAATTCTTGCTTAAGTAATGACGGTAGGAGCAGATCGGCCGGTAAATTCTTGCATTTTTGA

Protein sequence

MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANGAKVFPPNKHSGSTYSDVLRDLAPPQASNNYFFKIMGNSCLSNDGRSRSAGKFLHF
Homology
BLAST of CmoCh15G010080 vs. ExPASy Swiss-Prot
Match: O49298 (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.3e-142
Identity = 278/547 (50.82%), Postives = 357/547 (65.27%), Query Frame = 0

Query: 13  LGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQA 72
           + I+H  + H   SC QTPYP +C H +    N P   +        F +  + +T++QA
Sbjct: 25  VSISHLNA-HFITSCKQTPYPSVCDHHMS---NSPLKTLDDQTDGFTFHDLVVSSTMDQA 84

Query: 73  MVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMN-SMKANSIGHLTLL 132
           +  H  + S     S H      SAL DCLELYED+I +LN S  +    ++     T L
Sbjct: 85  VQLHRLVSSLKQHHSLH--KHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSL 144

Query: 133 SASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAA-VTATTSS 192
           SA++AN  TCR+GFRDF  +S Y  +  FP Q   N +K ISNSLA+TKAAA   A    
Sbjct: 145 SAAIANQDTCRNGFRDFKLTSSY--SKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEK 204

Query: 193 TPGS-------QRLLAG-------------SFPKWVSGDDRMLLRDT-TEKRTDMVVAQD 252
            P +       QR  AG              FP W    DR LL D+ T  + D+VVA+D
Sbjct: 205 YPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKD 264

Query: 253 GSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIV 312
           GSG++ +I +AV AAA++ + ++ R VIYVK G+YRENVVIKK++KNVM+IGDG+  TIV
Sbjct: 265 GSGHYTSIQQAVNAAAKLPRRNQ-RLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIV 324

Query: 313 TAMKNVKDGSTTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSF 372
           T  +NV+DG+TTFRSATFAV G GFIA+ +TFEN AG EKHQAVALR+++D S+FY CSF
Sbjct: 325 TGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSF 384

Query: 373 KGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRS 432
           KGYQDTLY HS+R F R C+IYGT+DFIFGD  ++ QNCNI+ RKPM  QKNT+TAQ R 
Sbjct: 385 KGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRK 444

Query: 433 DPNENTGFVIHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLP 492
           +P+E TGFVI +S V  A +         TYLGRPW+++SRTV M+  L  L+ PAGWLP
Sbjct: 445 EPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLP 504

Query: 493 WAGDFGLKTVYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGT 537
           W+G F L T+YYGE+ N+G GAS   RV+WPGYHV+ T  EAE+FTVE+FL+G  WI  T
Sbjct: 505 WSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITAT 553

BLAST of CmoCh15G010080 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 6.6e-129
Identity = 245/510 (48.04%), Postives = 329/510 (64.51%), Query Frame = 0

Query: 27  CSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMDLA 86
           C +TP P  C +F+        TDV      T F   +++  L +A  A      +  L 
Sbjct: 32  CGKTPNPQPCEYFL-----TQKTDVTSIKQDTDFYKISLQLALERATTAQSR---TYTLG 91

Query: 87  SFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKANSIGHLTLLSASLANHQTCRDGFR 146
           S   ++R K+A  DC ELYE ++ +LN++  +S     +   T LS +L N +TCR    
Sbjct: 92  SKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLE 151

Query: 147 DFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLLAGSFPKWV 206
           D G   + L        + +N +KLISN+L++ K           P ++      FP WV
Sbjct: 152 DLGVPEYVL------PLLSNNVTKLISNTLSLNK----------VPYNEPSYKDGFPTWV 211

Query: 207 SGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIYRE 266
              DR LL+  T  R ++VVAQDGSGN +TI EAVAAA+   +    R+VIY+K G Y E
Sbjct: 212 KPGDRKLLQ--TTPRANIVVAQDGSGNVKTIQEAVAAAS---RAGGSRYVIYIKAGTYNE 271

Query: 267 NVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAKDMTFENRAG 326
           N+ +K  LKN+M +GDG+G+TI+T  K+V  G+TTF+SAT AVVG+ FIA+D+T  N AG
Sbjct: 272 NIEVK--LKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAG 331

Query: 327 AEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGASVFQ 386
              HQAVALR+ +D S+FYRCSF+GYQDTLY HS R F+REC IYGT+DFIFG+ A V Q
Sbjct: 332 PNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQ 391

Query: 387 NCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVYKTYLGRPWK 446
           NCNIF RKP  N+ NT+TAQGR+DPN++TG +IHN  V AA DLK V+   KT+LGRPWK
Sbjct: 392 NCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWK 451

Query: 447 AYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGGGASTMRRVQWPGYHVMT 506
            YSRTV +++FLD LI PAGW+ W+GDF L T+YY E+ N+G G+ST  RV+W GYHV+T
Sbjct: 452 QYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLT 509

Query: 507 TAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           + ++  +FTV +F+ G  W+P T VPF +G
Sbjct: 512 SPSQVSQFTVGNFIAGNSWLPATNVPFTSG 509

BLAST of CmoCh15G010080 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 457.2 bits (1175), Expect = 2.8e-127
Identity = 249/510 (48.82%), Postives = 341/510 (66.86%), Query Frame = 0

Query: 27  CSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMDLA 86
           CSQTP P  C +F+   HN     +      + F   +++  L++A++A  H   +  L 
Sbjct: 34  CSQTPNPKPCEYFL--THNSNNEPIKSE---SEFLKISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKANSIGHLTLLSASLANHQTCRDGFR 146
               D R K+A  DC++LY+ +++++N ++  ++K + +   T LS +L N  TCR GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLLAGSFPKWV 206
           + G     + +I+ P  + +N S L+ N+LAI K        + TP  +      FP WV
Sbjct: 154 ELG-----VTDIVLP-LMSNNVSNLLCNTLAINK-----VPFNYTPPEK----DGFPSWV 213

Query: 207 SGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIYRE 266
              DR LL+ +T K  + VVA+DGSGNF+TI EA+ AA+        RFVIYVK G+Y E
Sbjct: 214 KPGDRKLLQSSTPK-DNAVVAKDGSGNFKTIKEAIDAAS-----GSGRFVIYVKQGVYSE 273

Query: 267 NVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAKDMTFENRAG 326
           N+ I+K  KNVM+ GDG+G+TI+T  K+V  G+TTF SAT A VG+GFIA+ +TF N AG
Sbjct: 274 NLEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAG 333

Query: 327 AEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGASVFQ 386
           A   QAVALR+ +D S+FY+CSF+ YQDTLY HSNR F+R+C +YGT+DFIFG+ A+V Q
Sbjct: 334 ASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQ 393

Query: 387 NCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVYKTYLGRPWK 446
           NCNIF R+P  ++ NT+TAQGRSDPN+NTG +IHNS V AA DL+ V G  KTYLGRPW+
Sbjct: 394 NCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWR 453

Query: 447 AYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGGGASTMRRVQWPGYHVMT 506
            YSRTV M++ LD LI+P GWL W G+F LKT++Y EF+N+G GAST  RV WPG+ V+ 
Sbjct: 454 QYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLG 510

Query: 507 TAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           +A+EA +FTV +FL GG WIP + VPF +G
Sbjct: 514 SASEASKFTVGTFLAGGSWIP-SSVPFTSG 510

BLAST of CmoCh15G010080 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 1.4e-118
Identity = 242/527 (45.92%), Postives = 320/527 (60.72%), Query Frame = 0

Query: 26  SCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLN--QAMVAHHHLLSSM 85
           SCS T YPDLCF  I          V ++      +   I  +LN     V H++     
Sbjct: 72  SCSSTRYPDLCFSAI--------AAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQK 131

Query: 86  DLASFHDDDRVKSALIDCLELYEDSITELNRSV--------MNSMKANSIGHLTLLSASL 145
            L   +   R K AL DCLE  ++++ EL+++V          S+  ++    TL+SA++
Sbjct: 132 LLKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAM 191

Query: 146 ANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQ 205
            N  TC DGF     + H + + +   Q+  +  K+ SN+LA+ K    T        + 
Sbjct: 192 TNQGTCLDGFSHDDANKH-VRDALSDGQV--HVEKMCSNALAMIKNMTDTDMMIMRTSNN 251

Query: 206 RLL------AGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRK 265
           R L         +P W+S  DR LL+ ++    + VVA DGSGNF+T++ AVAAA +   
Sbjct: 252 RKLTEETSTVDGWPAWLSPGDRRLLQ-SSSVTPNAVVAADGSGNFKTVAAAVAAAPQ--- 311

Query: 266 GSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAV 325
           G  +R++I +K G+YRENV + K  KN+M IGDG   TI+T  +NV DGSTTF+SAT AV
Sbjct: 312 GGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAV 371

Query: 326 VGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECH 385
           VGEGF+A+D+TF+N AG  KHQAVALR  AD S FY C    YQDTLY HSNR FF  C 
Sbjct: 372 VGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCL 431

Query: 386 IYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPD 445
           I GT+DFIFG+ A+V QNC+I  RKP   QKN VTAQGR+DPN+NTG VI  S + A  D
Sbjct: 432 IAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSD 491

Query: 446 LKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGG 505
           LK V+G + TYLGRPWK YSRTV+M+S +  LI PAGW  W G+F L T++YGE +NSG 
Sbjct: 492 LKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGA 551

Query: 506 GASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           GA T  RV+W G+ V+T+A EA+ FT  SF+ G  W+  TG PF+ G
Sbjct: 552 GAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLG 583

BLAST of CmoCh15G010080 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 5.3e-118
Identity = 236/521 (45.30%), Postives = 326/521 (62.57%), Query Frame = 0

Query: 27  CSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMDLA 86
           C +TPYPD C  + K NHN       Q   ++ FR   + A +++A+ A   L +S    
Sbjct: 38  CDKTPYPDPCKCYFK-NHN----GFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNC 97

Query: 87  SFHDDDRVKSALIDCLELYEDSITELNRSVMN-SMKANS------IGHLTLLSASLANHQ 146
           +   D + ++ L DC++LY D+I +LNR++   S KA +          T LS +L N +
Sbjct: 98  T---DSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTE 157

Query: 147 TCRDGFRDFGFSSHYLNNIIFPHQILSN--FSKLISNSLAITKAAAVTATTSSTPGSQRL 206
           TCR G  D       LN   F   I+SN   S LISN LA+  A        +T  +Q+ 
Sbjct: 158 TCRRGSSD-------LNVTDFITPIVSNTKISHLISNCLAVNGALLTAGNKGNTTANQK- 217

Query: 207 LAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVI 266
               FP W+S  D+ LLR     R ++VVA+DGSG+F T+  A+  A   RK +  RFVI
Sbjct: 218 ---GFPTWLSRKDKRLLRAV---RANLVVAKDGSGHFNTVQAAIDVAGR-RKVTSGRFVI 277

Query: 267 YVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAK 326
           YVK GIY+EN+ ++    ++M++GDGM  TI+T  ++V+ G TT+ SAT  + G  FIAK
Sbjct: 278 YVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLHFIAK 337

Query: 327 DMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFI 386
            +TF N AG  K QAVALR+++D SIFY+CS +GYQDTL  HS R F+REC+IYGT+DFI
Sbjct: 338 GITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGTVDFI 397

Query: 387 FGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVY 446
           FG+ A+VFQNC I  R+P++ Q N +TAQGR+DP +NTG  IHNS +  APDLK V G  
Sbjct: 398 FGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLKPVVGTV 457

Query: 447 KTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPW--AGDFGLKTVYYGEFKNSGGGASTMR 506
           KTY+GRPW  +SRTVV++++LD ++ P GW PW     FGL T++Y E+KN+G  +ST  
Sbjct: 458 KTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPASSTRW 517

Query: 507 RVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           RV W G+HV+  A++A  FTV  F+ G  W+P TG+PF +G
Sbjct: 518 RVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSG 535

BLAST of CmoCh15G010080 vs. ExPASy TrEMBL
Match: A0A6J1FKP5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111445093 PE=3 SV=1)

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 536/536 (100.00%), Postives = 536/536 (100.00%), Query Frame = 0

Query: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60
           MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF
Sbjct: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60

Query: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120
           RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM
Sbjct: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120

Query: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180
           KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK
Sbjct: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180

Query: 181 AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA 240
           AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA
Sbjct: 181 AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA 240

Query: 241 VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST 300
           VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST
Sbjct: 241 VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST 300

Query: 301 TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS 360
           TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS
Sbjct: 301 TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS 360

Query: 361 NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH 420
           NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH
Sbjct: 361 NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH 420

Query: 421 NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY 480
           NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY
Sbjct: 421 NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY 480

Query: 481 YGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           YGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG
Sbjct: 481 YGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 536

BLAST of CmoCh15G010080 vs. ExPASy TrEMBL
Match: A0A6J1IBL7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472075 PE=3 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.7e-292
Identity = 507/534 (94.94%), Postives = 513/534 (96.07%), Query Frame = 0

Query: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRN 62
           ML CFLLFSFLGITHAQSFH FMSCSQTPYPDLCFHFI PNHN+PTTDVHQSYHITRFRN
Sbjct: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60

Query: 63  SAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKA 122
           SAIRATLNQAMVAHHHLLS+MDLASF DD RVKSALIDCLELYEDSITELNRS+MNSM A
Sbjct: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120

Query: 123 NSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAA 182
           +SI HLTLLSASLANHQTCRDGFRDFGFSSHYL NIIFPHQILSNFSKLISNSLAITK A
Sbjct: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYL-NIIFPHQILSNFSKLISNSLAITKIA 180

Query: 183 AVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVA 242
           AVTATTSSTP SQR+LAGSFPKWVSGDDRMLLRDTT KR DMVVAQDGSGNFRTISEAVA
Sbjct: 181 AVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVA 240

Query: 243 AAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTF 302
           AAAEVRK SERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMG TIVTAMKNVKDGSTTF
Sbjct: 241 AAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTF 300

Query: 303 RSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNR 362
           RSATFAVVGEGFIAKDMTFENRAG EKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNR
Sbjct: 301 RSATFAVVGEGFIAKDMTFENRAGPEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNR 360

Query: 363 HFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNS 422
           HFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQ RSDPNENTGFVIHNS
Sbjct: 361 HFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNS 420

Query: 423 VVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYG 482
           VVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMR+ LDGLIEPAGWLPW GDFGLKTVYYG
Sbjct: 421 VVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRNVLDGLIEPAGWLPWGGDFGLKTVYYG 480

Query: 483 EFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           EFKNSGGGASTMRRVQWPGYHVM T AEAERFTVESFLEGGGWIPGTGVPF NG
Sbjct: 481 EFKNSGGGASTMRRVQWPGYHVMKTEAEAERFTVESFLEGGGWIPGTGVPFGNG 533

BLAST of CmoCh15G010080 vs. ExPASy TrEMBL
Match: A0A1S3CSS2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103504549 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.3e-233
Identity = 408/537 (75.98%), Postives = 453/537 (84.36%), Query Frame = 0

Query: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60
           M+ ++CFLLFS L I H +SF+NFMSC QTPYPDLCFH+I PN N+PT D+ ++Y  TRF
Sbjct: 1   MKFVYCFLLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRF 60

Query: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120
           RNSA++ATLNQA   H HL S MDL SF DD+R K ALIDCLE YEDSI +LNRSV +S 
Sbjct: 61  RNSAVQATLNQARKVHDHLRSIMDLGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSST 120

Query: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180
             NSI HLT+LSASLANHQTC DGFRDF  S    NN   P QI+SNFSKL+SNSLAITK
Sbjct: 121 SVNSIDHLTMLSASLANHQTCLDGFRDFDNS----NNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA 240
           AA   A++SST   Q L  G  P+WV  DD+ LLRD   K  DMVVAQDGSG+FRTISEA
Sbjct: 181 AAVAPASSSSTSCGQPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISEA 240

Query: 241 VAAAAEVRKGS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGS 300
           VAAA E RKGS   RFVIYVKGGIY+ENVVIKK++KNVMMIGDGM  TI+TAMKNV+DGS
Sbjct: 241 VAAAEEARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDGS 300

Query: 301 TTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAH 360
           TTF+SATFAV GEGFIAKDMTFEN AG EKHQAVALR+NADRSIFYRCSFKGYQDTLYAH
Sbjct: 301 TTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAH 360

Query: 361 SNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVI 420
           SNR F+RECHIYGT+DFIFGDGA VFQNCNIFVRKPM NQKNT+TAQGRSDPNENTGFVI
Sbjct: 361 SNRQFYRECHIYGTVDFIFGDGAVVFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFVI 420

Query: 421 HNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTV 480
           HNSV++AAPDLK V+G+Y TYLGRPWKAYSRTV+M+S+LDGLIEPAGWLPWAGDFGL+T+
Sbjct: 421 HNSVIKAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRTI 480

Query: 481 YYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           YYGEF N GGGA+T  RV+WPGYHVMT+A EAE FTVESFL G GWIP TGVPF NG
Sbjct: 481 YYGEFMNIGGGANTKGRVRWPGYHVMTSATEAEGFTVESFLGGRGWIPTTGVPFVNG 533

BLAST of CmoCh15G010080 vs. ExPASy TrEMBL
Match: A0A0A0KUU2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G568300 PE=3 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 1.8e-233
Identity = 408/538 (75.84%), Postives = 455/538 (84.57%), Query Frame = 0

Query: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60
           M++LHCFLLFS L I H +SF+NFMSCSQTPYPDLCFH+I PN N+ T  + ++Y ITRF
Sbjct: 1   MKILHCFLLFSLLVIIHGRSFNNFMSCSQTPYPDLCFHYINPNDNIRTAHIDETYLITRF 60

Query: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120
           RNSAI+ATL QAM    HL S+MDL SF D+DR   ALIDCLE YEDSI ELNRS ++S 
Sbjct: 61  RNSAIQATLYQAMKVRDHLRSTMDLGSFDDNDRNHVALIDCLEFYEDSIAELNRSTLSST 120

Query: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180
            ANSI H T+LSASLANHQTC DGFRDFGF     NN   P QI+SNFSKL+SNSLAITK
Sbjct: 121 SANSIDHSTMLSASLANHQTCLDGFRDFGFLVDDSNNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 A-AAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISE 240
           A AA  +T+SST   Q LL G  P+WV  DD+ LL+D   K  DMVVAQDGSG+FRTISE
Sbjct: 181 AIAAAPSTSSSTSCGQPLLDGLCPEWVYDDDKTLLQDMVGKGADMVVAQDGSGDFRTISE 240

Query: 241 AVAAAAEVRKGS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDG 300
           AVAAA E RKGS   RFVIYVKGGIY+ENVVI+K +KN+MM+GDGM  TIVTAMKNV+DG
Sbjct: 241 AVAAAEEARKGSGSGRFVIYVKGGIYKENVVIEKRMKNIMMVGDGMDRTIVTAMKNVQDG 300

Query: 301 STTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYA 360
           STTF+SATFAV GEGFIAKDMTFEN AG EKHQAVALR+NADRSIFYRCSFKGYQDTLYA
Sbjct: 301 STTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYA 360

Query: 361 HSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFV 420
           HSNR F+RECHIYGT+DFIFGDG  VFQNCNIFVRKPM NQKNT+TAQ RSDPNEN+GFV
Sbjct: 361 HSNRQFYRECHIYGTVDFIFGDGVVVFQNCNIFVRKPMLNQKNTITAQARSDPNENSGFV 420

Query: 421 IHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKT 480
           IHNSV+ AAPDLK V+G+Y TYLGRPWKAYSRTV+M+S++DGLIEPAGWLPWAGDFGL+T
Sbjct: 421 IHNSVIGAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYMDGLIEPAGWLPWAGDFGLRT 480

Query: 481 VYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           VYYGEF N+G GA+T  RV+WPGYHVMT+A EAERFTVESFLEGGGWIP TGVPF NG
Sbjct: 481 VYYGEFMNTGDGANTKGRVEWPGYHVMTSAVEAERFTVESFLEGGGWIPATGVPFVNG 538

BLAST of CmoCh15G010080 vs. ExPASy TrEMBL
Match: A0A5D3E4L0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00430 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 6.1e-226
Identity = 395/513 (77.00%), Postives = 435/513 (84.80%), Query Frame = 0

Query: 25  MSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMD 84
           MSCSQTPYPDLCFH+I PN N+PT D+ ++Y  TRFRNSA++ATLNQA   H HL S MD
Sbjct: 1   MSCSQTPYPDLCFHYINPNDNIPTADIDETYLNTRFRNSAVQATLNQARKVHDHLRSIMD 60

Query: 85  LASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKANSIGHLTLLSASLANHQTCRDG 144
           L SF DD+R K ALIDCLE YEDSI +LNRS  +S   NSI HLT+LSASLANHQTC DG
Sbjct: 61  LGSFDDDNRNKVALIDCLEFYEDSIAKLNRSAFSSTIVNSIDHLTMLSASLANHQTCLDG 120

Query: 145 FRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLLAGSFPK 204
           FRDF  S    NN   P QI+SNFSKL+SNSLAITKAA   A++SST   Q LL G  P+
Sbjct: 121 FRDFDNS----NNFFLPIQIMSNFSKLVSNSLAITKAAVAPASSSSTSCGQPLLDGLCPE 180

Query: 205 WVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGS-ERRFVIYVKGGI 264
           WV  DD+ LLRD   K  DMVVAQDGSG+FRTISEAVAAA E RKGS   RFVIYVKGGI
Sbjct: 181 WVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISEAVAAAEEARKGSGSERFVIYVKGGI 240

Query: 265 YRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAKDMTFEN 324
           Y+ENVVIKK++KNVMMIGDGM  TI+TAMKNV+DGSTTF+SATFAV GEGFIAKDMTFEN
Sbjct: 241 YKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDGSTTFQSATFAVAGEGFIAKDMTFEN 300

Query: 325 RAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGAS 384
            AG EKHQAVALR+NADRSIFYRCSFKGYQDTLYAHSNR F+RECHIYGT+DFIFGDGA 
Sbjct: 301 TAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAHSNRQFYRECHIYGTVDFIFGDGAV 360

Query: 385 VFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVYKTYLGR 444
           VFQNCNIFVRKPM NQKNT+TAQGRSDPNENTGFVIHNSV++AAPDLK V+G+Y TYLGR
Sbjct: 361 VFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFVIHNSVIKAAPDLKPVQGLYGTYLGR 420

Query: 445 PWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGGGASTMRRVQWPGYH 504
           PWKAYSRTV+M+S+LDGLIEPAGWLPWAGDFGL+T+YYGEF N GGGA+T  RV+WPGYH
Sbjct: 421 PWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRTIYYGEFMNIGGGANTKGRVRWPGYH 480

Query: 505 VMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           VMT+A EAE FTVESFL G GWIP TGVPF NG
Sbjct: 481 VMTSATEAEGFTVESFLGGRGWIPTTGVPFVNG 509

BLAST of CmoCh15G010080 vs. NCBI nr
Match: XP_022939088.1 (pectinesterase [Cucurbita moschata])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 536/536 (100.00%), Postives = 536/536 (100.00%), Query Frame = 0

Query: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60
           MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF
Sbjct: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60

Query: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120
           RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM
Sbjct: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120

Query: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180
           KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK
Sbjct: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180

Query: 181 AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA 240
           AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA
Sbjct: 181 AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA 240

Query: 241 VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST 300
           VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST
Sbjct: 241 VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST 300

Query: 301 TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS 360
           TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS
Sbjct: 301 TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS 360

Query: 361 NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH 420
           NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH
Sbjct: 361 NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH 420

Query: 421 NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY 480
           NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY
Sbjct: 421 NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY 480

Query: 481 YGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           YGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG
Sbjct: 481 YGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 536

BLAST of CmoCh15G010080 vs. NCBI nr
Match: XP_023549991.1 (pectinesterase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.8e-302
Identity = 521/536 (97.20%), Postives = 526/536 (98.13%), Query Frame = 0

Query: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60
           M MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFI PNHNVPTTDVHQSY+ITRF
Sbjct: 1   MNMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFINPNHNVPTTDVHQSYNITRF 60

Query: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120
           RNSAIRATLNQAMVAHHHLLS+MDLASF DDDRVKSALIDCLELYEDSITELNRSVMNSM
Sbjct: 61  RNSAIRATLNQAMVAHHHLLSAMDLASFDDDDRVKSALIDCLELYEDSITELNRSVMNSM 120

Query: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180
           +ANSI HLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLI+NSLAITK
Sbjct: 121 RANSIDHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLITNSLAITK 180

Query: 181 AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA 240
           AAAVTATTSS P SQRLLAGSFPKWVS DDRMLLRDTTEKR DMVVAQDGSGNFRTISEA
Sbjct: 181 AAAVTATTSSNPRSQRLLAGSFPKWVSSDDRMLLRDTTEKRADMVVAQDGSGNFRTISEA 240

Query: 241 VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST 300
           VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST
Sbjct: 241 VAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGST 300

Query: 301 TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHS 360
           TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAV+LRTNADRSIFYRCSFKGYQDTLYAHS
Sbjct: 301 TFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVSLRTNADRSIFYRCSFKGYQDTLYAHS 360

Query: 361 NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH 420
           NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH
Sbjct: 361 NRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIH 420

Query: 421 NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY 480
           NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY
Sbjct: 421 NSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVY 480

Query: 481 YGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           YGEFKNSGGGASTMRRVQWPGYHVM TAAEAERFTVESFLEGGGWIP TGVPFANG
Sbjct: 481 YGEFKNSGGGASTMRRVQWPGYHVMKTAAEAERFTVESFLEGGGWIPATGVPFANG 536

BLAST of CmoCh15G010080 vs. NCBI nr
Match: XP_022973540.1 (pectinesterase [Cucurbita maxima])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.7e-292
Identity = 507/534 (94.94%), Postives = 513/534 (96.07%), Query Frame = 0

Query: 3   MLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRN 62
           ML CFLLFSFLGITHAQSFH FMSCSQTPYPDLCFHFI PNHN+PTTDVHQSYHITRFRN
Sbjct: 1   MLRCFLLFSFLGITHAQSFHTFMSCSQTPYPDLCFHFINPNHNIPTTDVHQSYHITRFRN 60

Query: 63  SAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKA 122
           SAIRATLNQAMVAHHHLLS+MDLASF DD RVKSALIDCLELYEDSITELNRS+MNSM A
Sbjct: 61  SAIRATLNQAMVAHHHLLSAMDLASFDDDYRVKSALIDCLELYEDSITELNRSMMNSMGA 120

Query: 123 NSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAA 182
           +SI HLTLLSASLANHQTCRDGFRDFGFSSHYL NIIFPHQILSNFSKLISNSLAITK A
Sbjct: 121 HSIDHLTLLSASLANHQTCRDGFRDFGFSSHYL-NIIFPHQILSNFSKLISNSLAITKIA 180

Query: 183 AVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVA 242
           AVTATTSSTP SQR+LAGSFPKWVSGDDRMLLRDTT KR DMVVAQDGSGNFRTISEAVA
Sbjct: 181 AVTATTSSTPYSQRILAGSFPKWVSGDDRMLLRDTTVKRADMVVAQDGSGNFRTISEAVA 240

Query: 243 AAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTF 302
           AAAEVRK SERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMG TIVTAMKNVKDGSTTF
Sbjct: 241 AAAEVRKASERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGATIVTAMKNVKDGSTTF 300

Query: 303 RSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNR 362
           RSATFAVVGEGFIAKDMTFENRAG EKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNR
Sbjct: 301 RSATFAVVGEGFIAKDMTFENRAGPEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNR 360

Query: 363 HFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNS 422
           HFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQ RSDPNENTGFVIHNS
Sbjct: 361 HFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQARSDPNENTGFVIHNS 420

Query: 423 VVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYG 482
           VVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMR+ LDGLIEPAGWLPW GDFGLKTVYYG
Sbjct: 421 VVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRNVLDGLIEPAGWLPWGGDFGLKTVYYG 480

Query: 483 EFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           EFKNSGGGASTMRRVQWPGYHVM T AEAERFTVESFLEGGGWIPGTGVPF NG
Sbjct: 481 EFKNSGGGASTMRRVQWPGYHVMKTEAEAERFTVESFLEGGGWIPGTGVPFGNG 533

BLAST of CmoCh15G010080 vs. NCBI nr
Match: XP_038906560.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 824.7 bits (2129), Expect = 5.1e-235
Identity = 420/539 (77.92%), Postives = 459/539 (85.16%), Query Frame = 0

Query: 1   MEMLHCFLLFSFL-GITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITR 60
           M+MLHC LLFS L  I H +SF+NFMSCSQTPYPDLCFH+I PN NV T D+ ++  ITR
Sbjct: 1   MKMLHCLLLFSLLFMIIHGKSFNNFMSCSQTPYPDLCFHYINPNDNVLTADIDETSVITR 60

Query: 61  FRNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNS 120
           ++N  IRATLNQAMV H HL  +MDL SF DDDR K ALIDCLELYEDSITELNR  M+S
Sbjct: 61  YQNLTIRATLNQAMVVHDHLRFAMDLDSFDDDDRTKFALIDCLELYEDSITELNRLAMSS 120

Query: 121 MKAN-SIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAI 180
           M  N SI HLTLLSASLANHQTC+DGFRDFG     L +   P QILSNFSKL+SNSLAI
Sbjct: 121 MSVNSSIDHLTLLSASLANHQTCQDGFRDFG-----LFSFDDPFQILSNFSKLVSNSLAI 180

Query: 181 TKAA-AVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTI 240
           TKAA AV  T+SST G +RLL GSFP+W+   DR+LL+ T   R DMVVAQDGSG+FRTI
Sbjct: 181 TKAAVAVPPTSSSTSGGRRLLGGSFPQWIYDVDRILLQGTVGNRADMVVAQDGSGDFRTI 240

Query: 241 SEAVAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKD 300
           SEAV AA +  KG  RRFVIYVKGGIY+ENVVIKK +KNVMMIGDGM  TIVTAMKNV+D
Sbjct: 241 SEAVTAAEDATKGC-RRFVIYVKGGIYKENVVIKKGMKNVMMIGDGMDRTIVTAMKNVQD 300

Query: 301 GSTTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLY 360
           GSTTF+SATFAV GEGFIAKDMTFEN AG EKHQAVALR+NADRSIFYRCSFKGYQDTLY
Sbjct: 301 GSTTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLY 360

Query: 361 AHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGF 420
           AHSNR F+RECHIYGT+DFIFGD  +VFQNCNIFVRKPM NQKNT+TA  RSDPNENTGF
Sbjct: 361 AHSNRQFYRECHIYGTVDFIFGDAVAVFQNCNIFVRKPMMNQKNTITAHARSDPNENTGF 420

Query: 421 VIHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLK 480
           VIHNSV++AAPDLK VEG+Y TYLGRPWK YSRTV+M+SFLDGLIEPAGWLPWAGDFGL+
Sbjct: 421 VIHNSVIQAAPDLKPVEGLYGTYLGRPWKPYSRTVIMKSFLDGLIEPAGWLPWAGDFGLR 480

Query: 481 TVYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           TVYYGEF N+GGGA T  RVQWPGYHVMT+AAEAERFTVESFLEGG WIP TGVPFANG
Sbjct: 481 TVYYGEFMNTGGGAYTKGRVQWPGYHVMTSAAEAERFTVESFLEGGSWIPATGVPFANG 533

BLAST of CmoCh15G010080 vs. NCBI nr
Match: XP_008467117.1 (PREDICTED: pectinesterase [Cucumis melo])

HSP 1 Score: 818.9 bits (2114), Expect = 2.8e-233
Identity = 408/537 (75.98%), Postives = 453/537 (84.36%), Query Frame = 0

Query: 1   MEMLHCFLLFSFLGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRF 60
           M+ ++CFLLFS L I H +SF+NFMSC QTPYPDLCFH+I PN N+PT D+ ++Y  TRF
Sbjct: 1   MKFVYCFLLFSLLVIIHGRSFNNFMSCFQTPYPDLCFHYINPNDNIPTADIDETYLNTRF 60

Query: 61  RNSAIRATLNQAMVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMNSM 120
           RNSA++ATLNQA   H HL S MDL SF DD+R K ALIDCLE YEDSI +LNRSV +S 
Sbjct: 61  RNSAVQATLNQARKVHDHLRSIMDLGSFDDDNRNKVALIDCLEFYEDSIAKLNRSVFSST 120

Query: 121 KANSIGHLTLLSASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITK 180
             NSI HLT+LSASLANHQTC DGFRDF  S    NN   P QI+SNFSKL+SNSLAITK
Sbjct: 121 SVNSIDHLTMLSASLANHQTCLDGFRDFDNS----NNFFLPIQIMSNFSKLVSNSLAITK 180

Query: 181 AAAVTATTSSTPGSQRLLAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEA 240
           AA   A++SST   Q L  G  P+WV  DD+ LLRD   K  DMVVAQDGSG+FRTISEA
Sbjct: 181 AAVAPASSSSTSCGQPLPDGLCPEWVYDDDKTLLRDMVGKGADMVVAQDGSGDFRTISEA 240

Query: 241 VAAAAEVRKGS-ERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGS 300
           VAAA E RKGS   RFVIYVKGGIY+ENVVIKK++KNVMMIGDGM  TI+TAMKNV+DGS
Sbjct: 241 VAAAEEARKGSGSERFVIYVKGGIYKENVVIKKSMKNVMMIGDGMDRTIITAMKNVQDGS 300

Query: 301 TTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAH 360
           TTF+SATFAV GEGFIAKDMTFEN AG EKHQAVALR+NADRSIFYRCSFKGYQDTLYAH
Sbjct: 301 TTFQSATFAVAGEGFIAKDMTFENTAGPEKHQAVALRSNADRSIFYRCSFKGYQDTLYAH 360

Query: 361 SNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVI 420
           SNR F+RECHIYGT+DFIFGDGA VFQNCNIFVRKPM NQKNT+TAQGRSDPNENTGFVI
Sbjct: 361 SNRQFYRECHIYGTVDFIFGDGAVVFQNCNIFVRKPMMNQKNTITAQGRSDPNENTGFVI 420

Query: 421 HNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTV 480
           HNSV++AAPDLK V+G+Y TYLGRPWKAYSRTV+M+S+LDGLIEPAGWLPWAGDFGL+T+
Sbjct: 421 HNSVIKAAPDLKPVQGLYGTYLGRPWKAYSRTVIMKSYLDGLIEPAGWLPWAGDFGLRTI 480

Query: 481 YYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           YYGEF N GGGA+T  RV+WPGYHVMT+A EAE FTVESFL G GWIP TGVPF NG
Sbjct: 481 YYGEFMNIGGGANTKGRVRWPGYHVMTSATEAEGFTVESFLGGRGWIPTTGVPFVNG 533

BLAST of CmoCh15G010080 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-143
Identity = 278/547 (50.82%), Postives = 357/547 (65.27%), Query Frame = 0

Query: 13  LGITHAQSFHNFMSCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQA 72
           + I+H  + H   SC QTPYP +C H +    N P   +        F +  + +T++QA
Sbjct: 25  VSISHLNA-HFITSCKQTPYPSVCDHHMS---NSPLKTLDDQTDGFTFHDLVVSSTMDQA 84

Query: 73  MVAHHHLLSSMDLASFHDDDRVKSALIDCLELYEDSITELNRSVMN-SMKANSIGHLTLL 132
           +  H  + S     S H      SAL DCLELYED+I +LN S  +    ++     T L
Sbjct: 85  VQLHRLVSSLKQHHSLH--KHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSL 144

Query: 133 SASLANHQTCRDGFRDFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAA-VTATTSS 192
           SA++AN  TCR+GFRDF  +S Y  +  FP Q   N +K ISNSLA+TKAAA   A    
Sbjct: 145 SAAIANQDTCRNGFRDFKLTSSY--SKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEK 204

Query: 193 TPGS-------QRLLAG-------------SFPKWVSGDDRMLLRDT-TEKRTDMVVAQD 252
            P +       QR  AG              FP W    DR LL D+ T  + D+VVA+D
Sbjct: 205 YPSTGFTKFSKQRSSAGGGSHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKD 264

Query: 253 GSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIYRENVVIKKTLKNVMMIGDGMGETIV 312
           GSG++ +I +AV AAA++ + ++ R VIYVK G+YRENVVIKK++KNVM+IGDG+  TIV
Sbjct: 265 GSGHYTSIQQAVNAAAKLPRRNQ-RLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIV 324

Query: 313 TAMKNVKDGSTTFRSATFAVVGEGFIAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSF 372
           T  +NV+DG+TTFRSATFAV G GFIA+ +TFEN AG EKHQAVALR+++D S+FY CSF
Sbjct: 325 TGNRNVQDGTTTFRSATFAVSGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSF 384

Query: 373 KGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRS 432
           KGYQDTLY HS+R F R C+IYGT+DFIFGD  ++ QNCNI+ RKPM  QKNT+TAQ R 
Sbjct: 385 KGYQDTLYLHSSRQFLRNCNIYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRK 444

Query: 433 DPNENTGFVIHNSVVRAAPDLKAVEGVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLP 492
           +P+E TGFVI +S V  A +         TYLGRPW+++SRTV M+  L  L+ PAGWLP
Sbjct: 445 EPDETTGFVIQSSTVATASE---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLP 504

Query: 493 WAGDFGLKTVYYGEFKNSGGGASTMRRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGT 537
           W+G F L T+YYGE+ N+G GAS   RV+WPGYHV+ T  EAE+FTVE+FL+G  WI  T
Sbjct: 505 WSGSFALSTLYYGEYGNTGAGASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITAT 553

BLAST of CmoCh15G010080 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 457.2 bits (1175), Expect = 2.0e-128
Identity = 249/510 (48.82%), Postives = 341/510 (66.86%), Query Frame = 0

Query: 27  CSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMDLA 86
           CSQTP P  C +F+   HN     +      + F   +++  L++A++A  H   +  L 
Sbjct: 34  CSQTPNPKPCEYFL--THNSNNEPIKSE---SEFLKISMKLVLDRAILAKTH---AFTLG 93

Query: 87  SFHDDDRVKSALIDCLELYEDSITELNRSVMNSMKANSIGHLTLLSASLANHQTCRDGFR 146
               D R K+A  DC++LY+ +++++N ++  ++K + +   T LS +L N  TCR GF 
Sbjct: 94  PKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFL 153

Query: 147 DFGFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLLAGSFPKWV 206
           + G     + +I+ P  + +N S L+ N+LAI K        + TP  +      FP WV
Sbjct: 154 ELG-----VTDIVLP-LMSNNVSNLLCNTLAINK-----VPFNYTPPEK----DGFPSWV 213

Query: 207 SGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVIYVKGGIYRE 266
              DR LL+ +T K  + VVA+DGSGNF+TI EA+ AA+        RFVIYVK G+Y E
Sbjct: 214 KPGDRKLLQSSTPK-DNAVVAKDGSGNFKTIKEAIDAAS-----GSGRFVIYVKQGVYSE 273

Query: 267 NVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAKDMTFENRAG 326
           N+ I+K  KNVM+ GDG+G+TI+T  K+V  G+TTF SAT A VG+GFIA+ +TF N AG
Sbjct: 274 NLEIRK--KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAG 333

Query: 327 AEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFGDGASVFQ 386
           A   QAVALR+ +D S+FY+CSF+ YQDTLY HSNR F+R+C +YGT+DFIFG+ A+V Q
Sbjct: 334 ASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQ 393

Query: 387 NCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVYKTYLGRPWK 446
           NCNIF R+P  ++ NT+TAQGRSDPN+NTG +IHNS V AA DL+ V G  KTYLGRPW+
Sbjct: 394 NCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWR 453

Query: 447 AYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGGGASTMRRVQWPGYHVMT 506
            YSRTV M++ LD LI+P GWL W G+F LKT++Y EF+N+G GAST  RV WPG+ V+ 
Sbjct: 454 QYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLG 510

Query: 507 TAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           +A+EA +FTV +FL GG WIP + VPF +G
Sbjct: 514 SASEASKFTVGTFLAGGSWIP-SSVPFTSG 510

BLAST of CmoCh15G010080 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 426.4 bits (1095), Expect = 3.7e-119
Identity = 236/521 (45.30%), Postives = 326/521 (62.57%), Query Frame = 0

Query: 27  CSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMDLA 86
           C +TPYPD C  + K NHN       Q   ++ FR   + A +++A+ A   L +S    
Sbjct: 38  CDKTPYPDPCKCYFK-NHN----GFQQPTQLSEFRVMLVEAAMDRAISARAELTNSGKNC 97

Query: 87  SFHDDDRVKSALIDCLELYEDSITELNRSVMN-SMKANS------IGHLTLLSASLANHQ 146
           +   D + ++ L DC++LY D+I +LNR++   S KA +          T LS +L N +
Sbjct: 98  T---DSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSCTDFDAQTWLSTALTNTE 157

Query: 147 TCRDGFRDFGFSSHYLNNIIFPHQILSN--FSKLISNSLAITKAAAVTATTSSTPGSQRL 206
           TCR G  D       LN   F   I+SN   S LISN LA+  A        +T  +Q+ 
Sbjct: 158 TCRRGSSD-------LNVTDFITPIVSNTKISHLISNCLAVNGALLTAGNKGNTTANQK- 217

Query: 207 LAGSFPKWVSGDDRMLLRDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVI 266
               FP W+S  D+ LLR     R ++VVA+DGSG+F T+  A+  A   RK +  RFVI
Sbjct: 218 ---GFPTWLSRKDKRLLRAV---RANLVVAKDGSGHFNTVQAAIDVAGR-RKVTSGRFVI 277

Query: 267 YVKGGIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAK 326
           YVK GIY+EN+ ++    ++M++GDGM  TI+T  ++V+ G TT+ SAT  + G  FIAK
Sbjct: 278 YVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEGLHFIAK 337

Query: 327 DMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFI 386
            +TF N AG  K QAVALR+++D SIFY+CS +GYQDTL  HS R F+REC+IYGT+DFI
Sbjct: 338 GITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIYGTVDFI 397

Query: 387 FGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVY 446
           FG+ A+VFQNC I  R+P++ Q N +TAQGR+DP +NTG  IHNS +  APDLK V G  
Sbjct: 398 FGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLKPVVGTV 457

Query: 447 KTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPW--AGDFGLKTVYYGEFKNSGGGASTMR 506
           KTY+GRPW  +SRTVV++++LD ++ P GW PW     FGL T++Y E+KN+G  +ST  
Sbjct: 458 KTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGPASSTRW 517

Query: 507 RVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           RV W G+HV+  A++A  FTV  F+ G  W+P TG+PF +G
Sbjct: 518 RVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSG 535

BLAST of CmoCh15G010080 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 420.2 bits (1079), Expect = 2.7e-117
Identity = 233/522 (44.64%), Postives = 314/522 (60.15%), Query Frame = 0

Query: 26  SCSQTPYPDLCFHFIKPNHNVPTTDVHQSYHITRFRNSAIRATLNQAMVAHHHLLSSMDL 85
           +CS++ YP+LC   +       T D ++  HI      +  ATL +   A   L +S  +
Sbjct: 78  TCSKSLYPNLCIDTLLDFPGSLTADENELIHI------SFNATLQKFSKA---LYTSSTI 137

Query: 86  ASFHDDDRVKSALIDCLELYEDSITELNRS----VMNSMKANSIGHLTLLSASLANHQTC 145
                  RV+SA   CLEL +DS+  L R+    V+ S   +    +T LS+++ NH TC
Sbjct: 138 TYTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTC 197

Query: 146 RDGFRDF-GFSSHYLNNIIFPHQILSNFSKLISNSLAITKAAAVTATTSSTPGSQRLL-- 205
            DGF +  G      + +I     + + S+++SN LAI        +      +++LL  
Sbjct: 198 TDGFDEIEGQGGEVKDQVI---GAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGT 257

Query: 206 --AGSFPKWVSGDDRMLL-RDTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRF 265
                 P W+  +DR LL   T+  + D+ V++DGSG F+TI+EA+  A E    S RRF
Sbjct: 258 EETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPE---HSSRRF 317

Query: 266 VIYVKGGIY-RENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGF 325
           VIYVK G Y  EN+ + +   N+M IGDG G+T++T  K++ D  TTF +ATFA  G GF
Sbjct: 318 VIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGF 377

Query: 326 IAKDMTFENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTI 385
           I +DMTFEN AG  KHQAVALR   D ++ YRC+  GYQD LY HSNR FFREC IYGT+
Sbjct: 378 IVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTV 437

Query: 386 DFIFGDGASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVE 445
           DFIFG+ A + Q+CNI+ RKPM  QK T+TAQ R DPN+NTG  IH   + A PDL+A +
Sbjct: 438 DFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASK 497

Query: 446 GVYKTYLGRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGGGASTM 505
           G Y TYLGRPWK YSR V M S +   I+P GWL W G F L ++YYGE+ N G G+   
Sbjct: 498 GSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIG 557

Query: 506 RRVQWPGYHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           +RV+WPGYHV+T+  EA +FTV  F+ G  W+P TGV F +G
Sbjct: 558 QRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSG 584

BLAST of CmoCh15G010080 vs. TAIR 10
Match: AT3G60730.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 418.7 bits (1075), Expect = 7.8e-117
Identity = 221/455 (48.57%), Postives = 296/455 (65.05%), Query Frame = 0

Query: 95  KSALIDCLELYEDSITELNRSVMNSMKANSIGHLTLLSASLANHQTCRDGF--RDFGFSS 154
           K  L +C +LY++S   L++ V++          T LS  LANH TC DG   +  G   
Sbjct: 66  KLGLSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKP 125

Query: 155 HYLNNIIFP-HQILSNFSKLISNSLAITKAAAVTATTSSTP---------GSQRLLAGSF 214
              +N+ F  H+ L+ + K   +        A      + P         G  R   G  
Sbjct: 126 LVHSNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHG-- 185

Query: 215 PKWVSGDDRMLLR-DTTEKRTDMVVAQDGSGNFRTISEAVAAAAEVRKGSERRFVIYVKG 274
           P   + +  ML+  + T  R D VVA+DGS   RTI++A+AA + + K    R +IY+K 
Sbjct: 186 PSRPNQNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRLNRVIIYIKA 245

Query: 275 GIYRENVVIKKTLKNVMMIGDGMGETIVTAMKNVKDGSTTFRSATFAVVGEGFIAKDMTF 334
           G+Y E + I + +KN+M++GDGM  TIVT  +NV DGSTT+ SATF V G+GF A+D+TF
Sbjct: 246 GVYNEKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATFGVSGDGFWARDITF 305

Query: 335 ENRAGAEKHQAVALRTNADRSIFYRCSFKGYQDTLYAHSNRHFFRECHIYGTIDFIFGDG 394
           EN AG  KHQAVALR ++D S+FYRCSFKGYQDTL+ HS R F+R+CHIYGTIDFIFGD 
Sbjct: 306 ENTAGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDA 365

Query: 395 ASVFQNCNIFVRKPMENQKNTVTAQGRSDPNENTGFVIHNSVVRAAPDLKAVEGVYKTYL 454
           A+VFQNC+IFVR+PM++Q N +TAQGR DP+ N+G  I +S +RAAP+ +AV+G +K+YL
Sbjct: 366 AAVFQNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYL 425

Query: 455 GRPWKAYSRTVVMRSFLDGLIEPAGWLPWAGDFGLKTVYYGEFKNSGGGASTMRRVQWPG 514
           GRPWK YSRTV +++ +D LI+P GW  W+G + L T+YYGEF N+G GA T RRV WPG
Sbjct: 426 GRPWKKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPG 485

Query: 515 YHVMTTAAEAERFTVESFLEGGGWIPGTGVPFANG 537
           +HV+    EA  FTV  F++G  WIP TGVPF+ G
Sbjct: 486 FHVLRGEEEASPFTVSRFIQGDSWIPITGVPFSAG 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O492982.3e-14250.82Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=37... [more]
O048876.6e-12948.04Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221492.8e-12748.82Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
O048861.4e-11845.92Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Q9FHN55.3e-11845.30Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1FKP50.0e+00100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111445093 PE=3 SV=1[more]
A0A6J1IBL73.7e-29294.94Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472075 PE=3 SV=1[more]
A0A1S3CSS21.3e-23375.98Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103504549 PE=3 SV=1[more]
A0A0A0KUU21.8e-23375.84Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G568300 PE=3 SV=1[more]
A0A5D3E4L06.1e-22677.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00430... [more]
Match NameE-valueIdentityDescription
XP_022939088.10.0e+00100.00pectinesterase [Cucurbita moschata][more]
XP_023549991.12.8e-30297.20pectinesterase [Cucurbita pepo subsp. pepo][more]
XP_022973540.17.7e-29294.94pectinesterase [Cucurbita maxima][more]
XP_038906560.15.1e-23577.92pectinesterase [Benincasa hispida][more]
XP_008467117.12.8e-23375.98PREDICTED: pectinesterase [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G23200.11.7e-14350.82Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G45220.12.0e-12848.82Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.13.7e-11945.30Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.12.7e-11744.64pectin methylesterase PCR fragment F [more]
AT3G60730.17.8e-11748.57Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006633Carbohydrate-binding/sugar hydrolysis domainSMARTSM00722completecoord: 259..439
e-value: 0.0037
score: 26.5
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 16..178
e-value: 4.6E-23
score: 92.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 26..180
e-value: 1.9E-16
score: 57.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 26..178
e-value: 5.8E-22
score: 78.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 25..182
e-value: 4.3E-24
score: 87.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 26..180
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 217..537
e-value: 1.2E-124
score: 417.9
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 223..522
e-value: 1.5E-143
score: 477.0
NoneNo IPR availablePANTHERPTHR31707:SF152PECTINESTERASE INHIBITOR 5-RELATEDcoord: 21..536
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 21..536
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 26..176
e-value: 5.85028E-29
score: 110.606
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 251..270
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 370..379
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 222..534

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G010080.1CmoCh15G010080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity