CmoCh15G005730 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh15G005730
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptiontRNA pseudouridine(38/39) synthase isoform X1
LocationCmo_Chr15: 2731081 .. 2739632 (-)
RNA-Seq ExpressionCmoCh15G005730
SyntenyCmoCh15G005730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGTCTCACATCGGCTAAGGAATACTGTCTTCATTGGTGTGAGCTCTTTTGAGAAGCCCAAAGTAAAGCTACTAGAGCATCATACCATTGTGGGAGTCATGTTCATCTAACATGATATAAGAGTCATGCCCTAGATTTAACAATGTCAATAGAACGAAGAAAGGACACTAAAAGAAAAGGAGTCGAGCCTCGATTAAAGAGAGGTGTACTTTGTTCGAACCCAAATGTTGAATGAAAGTCCTACATCGGCTCGAGAGGCATACTTTGTTCGAGCCCACGTGTTGGATGAAAGTTTCACGTCCGCTGCTTAAGCCCACGGAAGATACCGTACATGGTCTGCTACGCAAAGTAGTACATCGGCGTGTATACTTGCTCTATAAATACGTTCCCGCCTTCTGACTTGGCTTTCTGAATAGACTATTTCGTCTCTTTCTTGTCGTCTTCAACCTCTGAATCTCGGTTTCCAGAGAAACGTCTCTGCTAGGGTTTTAGGGTTTCTTTTGTGATTTGCGGAATTTTTCCATTGCTGTAACATTGTGCTTCTTCTGTTCTTCAAGAAGCTTTCGTATGTGTTCTCTCGTCGTTTCTATGCGTCAATTCTGTTTTACCTCGCGATGAATTCCTAATTCTGTCTGAACTTTGGGGCCTTATAATTTGTTTTTCAGTGGTCGTTTGAACTCTGGGGCTGTATTTCTTGACGTACAAGGGCTAATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATTGGAAATCTTCAATCCCGGCTTCGTCTTCTCCAAGGCAGGGTTAAGGTTTGAATGTCTACTCTCATTTCATCTTCCGGTTGATTGATGAATTAATGGAGAAGTATCGGTAGAGACAAGACTGATGTTCGATCTCAATCCACTAATTTGTCAGGTTTTAGAGGAGGAAAATTCGAATCTATCCTCTCGACTTGCAACTTGCTGCTGTTCTGAGGTTTGGTCGACTATGTTAGAATGAAATCAGATCTATTTGGTTGTCATGCTAGCCCAGCTAACCTTAGGGTAGATTTTTCGTCAGGGCTAACTAAACATGAATTCGTGCAGAGGAGGATGAACTTTGACGATGGGAGTAGTTCGATTGAGAAAAGCAGGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCTGTAGAAAAAACGCCAGGCTAGTTGTTACTTCATGAACAAATAGAGTCTATTTTCTCATTCCTCCATAGTTGCGAAAATCAAATCAATTTTTTTCTTTTAGATAAGAGGATCACCAAAAAATGGTGCCATGGGGGAGTAGTAGTAGTAGTAGTAACTCACATGTAAATACGACCAAAGGAGGCAGAATGGAGGAGAATATAATATGTAACAGTGGAGTTCATATAAATATATTAATGAGATTTTTATGAAATTCAAGAATCAAGATTAAGAAATGATGATTTGTGAGTCTGAACTATAGTCAAATTATGCATTGACATACAATAAAATACTCAAATGTGGCTGTTTCACAATAACTGTTGCATATGGATTTGAAAATTTGCATGTCCCCAAATAGCCACTGGATAAACCAAATAGTTTTTCCTACCTGCCAGAGAAAAAGGTGAAAAATGATGAACCTTAGACATACGTTTGGAATGTTTATGAAGTTTAGATGAAATAATTTACGGTAGTTCTCATGCATATTATCAACCTTGTTTATCTACTGGAATGATTTGCTTGTTATTTTATGGGCGAAGGTTATAACACAAGGATCTTGGATCACTGTTCAAAGAGATATATTGCTCTAAAAGTAATGTATTTCGGGCGGAGGTATGTAATCTAGCGATAATGGGAACAATACCAGTACCTTTTACTTTCCCCTGCATTAATTTCCGTACATTTCAGTCCTCCGAAACCTTCTTCCGAGATATGGAGAAATCTTGTTAAAAGAAAACCCCACAACAGTTGTTATTTAAAAACTCTATCGTCGTAGCTTTTTGTTATACTGTGAAAATTTAACTTGATGTCAGATAGTGCCTGAAATTCTGGTTACTTCATCTGTTCAGGTTCTATGGCTTTGCTTCAGAGGCACAAATGGAACCAACAGTTGAGGTATAGTTTCAAATTAAATTGAACAAATTATTTATGATTTATCGATTACAGTGGTACTGATGGGGGGTACTTGTCAAGAACGCACAATATTGATTCTTAATGTTTACTGGGAGTGCCTAAACATTTAAATAATGCATTTAGAGGATCCCAATATCAATTCTTCCATCTTTTCTAATTGATATTTGATCACACCATCTGCTAGAATGAGCATCATGCGATTCTCAAGGGTAAATATAAGGTTTTCTTTGTCAATGAATATTCTTCCCTTCCTGCTTGCTAAGGAATGCCATCGAGATTATTTTGATGCTGTGTTTAATTATGGATTTGGATTATAATGAAGAATGCTAGAAAGCAAAATCGACATTCTGATTCATCTAACCATTAGGTTTTTGATGAGTTTGAGAAATAAATCGTGAATTTCTAAGTATGAATTATGAGGTAACCAGACACCCAAGATTTCAGGAGGCTTGGATTTAACTTGAGTTTCACTATAGTAATGTGTAGTCCTTCCCTCCTTTACCCTTGCAAGTCCTCCTTACAACCACCCTTTTTTCACCTTTCCCCTTCCTTTGTGATTCTCTTGCGATCCCAAAAGCAAACAAAAGGCTTAAGATGTAGATGGATCCAATAAAGGGGAGGGGACTAAAAAGAGTAGGAATTGTCACTGGAAAAGACTTTGACAAAGTTGATTGGCAACATTTGAATGCTACGTAAAAGTTGAAAGGCTTTAGGAAATAGATGAGCATGACTTCATGCGCAAGACCATGGTCTGGGTGGGTGTAAACATTTAGCATCAATTTGTTTTAAGGGGGAGGGGGTTACGGGAAGGTATATTTGGTGGTTGTGAGGAATGTTGGGTGAGAAGAGATTTACTTGTAAATTTTGTACCCAAAAAAAGAAGTTAATTTGTTATCGTTAATTTGTTATCTCATCTTCCTGCAGTCCGAAATTTTCAGAGCTCTTGATAAAGCCAGGCTTCTTGTTGGTGGTAGGAAGGAGTCTTGTTATTCAAGATGTGGTAGAACAGACAGGGGGGTCTCATCTGTTGGGCAAGTAAGACACTTAAAATCTGTAGAGAATAACATTGTACAGCTAGAATAGCATTGTATAACAGTAGCTTTATGCTAATTTTCTGAATACGTTGACTTCCTCATCTTTTTAATTAGGGAACTTTGTTGACCTTTCAATCCCTAATTGAGTTAATTTCACCTTTCTAGCAATCACACTATTGTTCCTGCAGGTAATTGCTCTCTATTTACGGTCCAACCTTAAGGATCCCCCTAATGGTGAAAATGGCAATCATAATGCGGAAGAGCACCATGGTCAGCTAAGTTGATGTTGCCAAAGTCGTTCTTGTTTGTTAATAACTTGAATACTAATCAACTGAATGATAATATGGAGTGCGCCAGACTTCTTGCTTTCTGTTACGGATAGCACTCTTGTTTGAAACACCAAAATATAGCAATATTCTTTACTGCATTAGGAAAGTAGATGGTAGCGATACTTTATACAGGGAACTACAGACATGACCCTTTCTTTTATTTTTTCAAAGGATGTCATCTATAGTTTTATGTGCTTATTATTATTTTTTAATTAACATTTTCAATTGGGGTAGTTTTTTCTTTCTAAGTTCTAATTGGAACGTTGCATACTTTTTCCATCTCCATTATCTCAACTTCATTTCCATTTATTATTTACTCTCAGATGGAGAAATTGATTATGTGAGAGTAATAAATAGAGTTCTTCCCAATGACATTCGGGTTTTAGGCTGGTGTACTGTTCCTGTTGGCTTCAGTGCAAGGTTTGTATTGCTCGTGATTTTATTTTTATTTTTTATTTATATAGATTTGGGTATTTTCGTTTTCTTGAGAGGAATGTGATGTGTTGATTTTTAATGTGAAGTCAGGAACTTCATGAGACTAGCATTCAACAGATTTAAAAATCAATATAACTGGCTTTGACTGCTGAGTTTATGTGCCTACATGAAATTCTGTAGAGTTGTCTTGCTATTCTAAATGTTTGTACACCTCTCTAAGAAGTGCAGTGACCAGTAGCATCTCCGTTACCCCTTACGTCATACAAGAAGCTTTACTTGTGCGTTATTTTTTTTCATTTCTTTGTTAGACACAGCTGGATTTAGGGTTTAAGTTCATTTCTTTGTTAGATACATGCTGCTTAAGTTCTCTAGACATGCTTACCTTTGCCAATGTATGGCATCAATGTCTGGGGTGACATTTTTTTTGTTACGCTGGGGCATTTGTTTGAAACCAAGAACCATGTTAATTTTTTGGTTACTTTCTAAAAATTGAGTTTACACATCTTACATTTTTGCTAAAGATTAACATTTTTCTTCCAAGGGACTGGATCAATGGGGGTTATGGTTCATAATTATGTTAAAAATTAGTTTTGGAAAAGACACATAGTAATTTATACCTAAACAATTCAAGAGTATTCAAATCTACATTTTAAGGTGTTATACTTGATAATCATGGGTACGTCATGATGCTCCCCATACACTAATACTCAATGCATGACCAATACTTCTTTGAGGAACTTATTATGTGAATTTGTTCCCGAAGTGTAAAACTTATCTATTCAGCATAATTTTTCAGCAAGAATCTTTAAGGTATGAATCTGTTGGAAAGACTCCAGTTCTAGTTTCTAACATACATGCTTATATTATCGGAAAAATATTAATGCTTACTCTGGCATTTCTTGTTGCCTCTTTATGCAGCACAGATTTAGTTGTTTAAGAAGAAAGTATAAATATTTATTCTGGGGAGCAAACTTGAACATCTCGGTATGCAAATTATTTTGTACGAGTCTTGTAATGCTTTTGCAGAAGTAATTTGGATGCTGCTTCAACAAAAGGTTGAAAAATTTTATCTGCATTTTCACCAGGCTATGGAGAGGGCCGGTAAGAAATTTGTAGGGGAACATGACTTCAGAAACTTCTGCAAAATGGATGCAGCAAATGTTCACAATTATAGGCGATGCATCATGTCATTTGACATTTCTCCTTGTGATGTGAGGTTGGCGTTGTTTTCAGATTTCTAATCTGTTTTCCAATTCAACTTTTCGTACCATGCTTAATTGAAACATGCCTTGCATTTGTATATTTTTATTGTCAAATCTGATTAGCATATTTGCTTCTCAGGCCATCCAAAAGAGCCTTCCTCTAGCTGTTGTTCAAGCTTATTATACTTTATTCTCCCTGTTTTTGGTCTCTTAAGTTCATTAACTATGTTCTCTCCTAGGTGTTGCCAAGCTGCCAGTAGTTTACTAACTAGAATAAGAGTGCTTTTCTGACCATGTTCATTTTGAGTTGTTGATAATCATGTTGAAATTGACCATCTCTGTGGTTTTTATCATTTGCGGGGGTAGATTCATATTATGCAAGATGCTTTGAACTTCAGAAGAATGATCATGTTAACTTTTTAAGCGTCCTGCAGTTATTGCTGTGTTAGATTTCTATTTATTCCAAATCTAGGATGCATTAAGACGACTACGCAAATTAAAAGAATGTGAAATATACTTGTGGGAGAAAGAAGCGTGGTACCATAGAACCCGTGTTTTATGATAGAATTCTATTGGATATATCTTTTGTAACTTCATTCTACATCATCGTGTCCTGAGCTAAGATGTCTTCTTGGCTCAAGACATCTACTTTTTTTTTTTTTGGTAAACAAATTAATCTCTCTCTCTCTCTCTCTCTCACAATCTTTTTAGTCTCTGAATCTTTTTGGTACTCTGACAGGATGGAAGGCAATCTATTATGGGCATTCAACATAGAAGGCAGTGCTTTCCTCTGGCATCAGGTCCGCTGCATGGTTGCTGTAGTATTCATGGTTGGGCAAGGTCTTGAAGATCCTGATGTGCGTGCCAGACGTCTTGACATCTGATTGCTTTATTATATTTGCATAAACACTTTTAATATGTTAAACTGCTTGCTTTTGCTCATGAAAATGTAGTCATCTTATACTTCACAGGCCTCAACACTCTCATTTCAGCTTATTATATCTCTAGAATGGCCATAAAAGATAAACAACTAATGGTAAATTTGGTAACTAGGAGCTAGTGTTTTCCAGAATTGATAGCCGTATTTATTTTTATATAGATGCATCTGCCGTTGACAATGAACCTTCCTAGCCACTGTTTCTTTTCAATACATGTGCACTTCCCCCCTTTACCGAAGTTATCAATCTTATTTCTTGGTTGGTAAACCCTAAGCATGACTTCGTTGCTCTTTTGAAATATGTGTTGTTTTGCTAATGGTCACACAATTAGCACTCATGTCAAGATTTCAACATTTAAAGTCGCTGTACACAGTGGTTTCCCAATTATCAGCTTTACTTGGCCTGTGGTGTGATTTTGTTATGCTATGTGAAGTATAAAATGCATCTCAATCTATTATATGAAGAGGACTGGTAAATTTTGTAGGTGGTTGATATTCTACTGGATCCATTAAGAACACCAAGGAAGCCTCAATATGTTATGGCTTCAGAAAATCCTCTGGTTCTTCATTCTTGTGAATTTGAAGGTGTCAAATTTAGGTGTTCAACAGGTACTTTTCTTCAGCTTCATTAAGGGATTAATTGATTATAGATAACGTGACTTCTACCTCCTGAATATTTTCTTTTTCACCTCAGTTGTCATTATATTATCGTTCTATATTTAAAGGATTTCCATACCTGCATTTCTCCTAGATGCTGGAGAAGCTCTACAGTTGCATTTATCAAATGAAATCCGCACATATGAGCTCCAAGCTGCAATATTCAAAGAAGCTCTTCTAAGCTGTTTGCCAATCACAGATGGTTTGAATTGCAATCTTTCCCTATTATACATTTTGTCATTATTTTTCAGTTGATTCATGATACTGATGTGGCAACTTCTATCTCCTCTCCAGATGAGAGCCTTTCAGACAACAGAAAGATAAAGAAGAAAATTTGCCATGTTCCCCTATTGTCAAGACCAACTGAACGTAAGTTCTGCACAAAAAGACTGCTGACTATTGGGATTATTTATTTGATAATGTCATATAGTACTACACTATCTGTGATTCCGTATATAACCAATGACTAAACAACTGAATATATTATTTTAGTATCACAGTTAAAAAAGAAAAACACTTCAGCTAAACGCAAATCCAATGACCCTTGTGTACATGTCAGTTAATAATTTGGTGTGAGCTGTTTTGTCCATAACCATTTACATAATGTTTTTAGTTAATGTGGAGCTTTTGTTTTGTCCATAGCAGAATCATCTCTCTTTCTTTGCTAATGATTTATGGGCCAGTAAATCTAGCTGTCATGGAGGAAGATGCTCATCGAGAAATGTGAGATCCCACATAGGTTGGAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTCCTTAGTAAACGTATTTTAAAACTTTGAGGGAAAGTCTTTGAAAGAAAGCCCAAAGAAGACAATATTTGCTAGCAGCAGATTGGTCTGTTACAAGAAATTAAAATATTAGCTACAAGATTGGTTTTCATTTAATTTATTTGATTTATTGAATCACTCTATCTTAAGCGTTGCATATTCCCCTTCTCATCCCCTAACTATATTATTAACTACCTCCAGAAGCATACCCTTCCCTCTGTTATTTCAATATGTTAGTTTATATGTATTGGTTTATGCTGTTCCGTTTCCAGCTTCTTACGAAGAGCGGCGTGCGAAGCTCAACTCATCCTCAAAGAGTTCATCAAATACATCTTCATAGGCACAGGTAACATAGTTTTCGTGTTTTTTGAAAAAAATAAATAAATCTATTAAGCATCTGTTTCTAAGATCTAATTTCTTCCCTTTCAAAAAGATATCAAGTGAACATGAAAGCCTAATTGGACTAATTATGATACTATATGGATACAAGATTATATCTTAGAGCAGCTCTCATCCCATTTCATAAGAGACTGAAGTCTTTTAAAACTGCATTTGGCGTAACTTTTAGAATACTAATTAAGGAACTTCTCAAATCTACTGTTCAATTCTTGAAATTTATGTTTATTTCTTATTATAACTCAAAGAAATATTTGAATTAGTGTACAATAAGGGGGTAAATTTAAAGATAATGAGTGTATCTGTAGTTTTGCAGAGTTTGAACGCCATTAAAAGAGCAGAAGCGGCAATGATGAGCATCCGGTGATGAAGGGGAGACGAGTCTAAAGAAAAAGCTGACAGCCCAACCACACCCCAACATCAACATGTACCATCATCATCATGAATCCAGGTGCATGAACTCCGACCAAATCATTTTTCTCACTTGCCCTGTCCCCCTCTCCTCTATGGTTTTATTAATGTTGAGAATGACTTGTGACGGTTCTGTGCTGTGTGTTTTTGTCCTGAACCATGTGAAAGCTTAA

mRNA sequence

ATGAAAGTCTCACATCGGCTAAGGAATACTGTCTTCATTGGTGTGAGCTCTTTTGAGAAGCCCAAAAAGCTTTCTGGTCGTTTGAACTCTGGGGCTGTATTTCTTGACGTACAAGGGCTAATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATTGGAAATCTTCAATCCCGGCTTCGTCTTCTCCAAGGCAGGGTTAAGGTTTTAGAGGAGGAAAATTCGAATCTATCCTCTCGACTTGCAACTTGCTGCTGTTCTGAGAGGAGGATGAACTTTGACGATGGGAGTAGTTCGATTGAGAAAAGCAGGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCTGTAGAAAAAACGCCAGGTTATAACACAAGGATCTTGGATCACTGTTCAAAGAGATATATTGCTCTAAAAGTAATGTATTTCGGGCGGAGGTTCTATGGCTTTGCTTCAGAGGCACAAATGGAACCAACAGTTGAGTCCGAAATTTTCAGAGCTCTTGATAAAGCCAGGCTTCTTGTTGGTGGTAGGAAGGAGTCTTGTTATTCAAGATGTGGTAGAACAGACAGGGGGGTCTCATCTGTTGGGCAAGTAATTGCTCTCTATTTACGGTCCAACCTTAAGGATCCCCCTAATGGTGAAAATGGCAATCATAATGCGGAAGAGCACCATGATGGAGAAATTGATTATGTGAGAGTAATAAATAGAGTTCTTCCCAATGACATTCGGGTTTTAGGCTGGTGTACTGTTCCTGTTGGCTTCAGTGCAAGATTTAGTTGTTTAAGAAGAAAGTATAAATATTTATTCTGGGGAGCAAACTTGAACATCTCGGCTATGGAGAGGGCCGGTAAGAAATTTGTAGGGGAACATGACTTCAGAAACTTCTGCAAAATGGATGCAGCAAATGTTCACAATTATAGGCGATGCATCATGTCATTTGACATTTCTCCTTGTGATGTGAGGATGGAAGGCAATCTATTATGGGCATTCAACATAGAAGGCAGTGCTTTCCTCTGGCATCAGGTCCGCTGCATGGTTGCTGTAGTATTCATGGTTGGGCAAGGTCTTGAAGATCCTGATGTGGTTGATATTCTACTGGATCCATTAAGAACACCAAGGAAGCCTCAATATGTTATGGCTTCAGAAAATCCTCTGGTTCTTCATTCTTGTGAATTTGAAGGTGTCAAATTTAGGTGTTCAACAGATGCTGGAGAAGCTCTACAGTTGCATTTATCAAATGAAATCCGCACATATGAGCTCCAAGCTGCAATATTCAAAGAAGCTCTTCTAAGCTGTTTGCCAATCACAGATGATGAGAGCCTTTCAGACAACAGAAAGATAAAGAAGAAAATTTGCCATGTTCCCCTATTGTCAAGACCAACTGAACCTTCTTACGAAGAGCGGCGTGCGAAGCTCAACTCATCCTCAAAGACATCCGGTGATGAAGGGGAGACGAGTCTAAAGAAAAAGCTGACAGCCCAACCACACCCCAACATCAACATGTACCATCATCATCATGAATCCAGGTGCATGAACTCCGACCAAATCATTTTTCTCACTTGCCCTGTCCCCCTCTCCTCTATGGTTTTATTAATGTTGAGAATGACTTGTGACGGTTCTGTGCTGTGTGTTTTTGTCCTGAACCATGTGAAAGCTTAA

Coding sequence (CDS)

ATGAAAGTCTCACATCGGCTAAGGAATACTGTCTTCATTGGTGTGAGCTCTTTTGAGAAGCCCAAAAAGCTTTCTGGTCGTTTGAACTCTGGGGCTGTATTTCTTGACGTACAAGGGCTAATGGACGGAGCGGAACATTCCCTCGATTACAGAGGAGGTACGGGAGCAATGGAAACGACCTCAGGAACTGACGAGTTTCAACCCTTCGATGGAAACCTAATTGGAAATCTTCAATCCCGGCTTCGTCTTCTCCAAGGCAGGGTTAAGGTTTTAGAGGAGGAAAATTCGAATCTATCCTCTCGACTTGCAACTTGCTGCTGTTCTGAGAGGAGGATGAACTTTGACGATGGGAGTAGTTCGATTGAGAAAAGCAGGAAATATGGAAGTCTGAAAGACAAGACTACTAAGAAGAAAGCTGTAGAAAAAACGCCAGGTTATAACACAAGGATCTTGGATCACTGTTCAAAGAGATATATTGCTCTAAAAGTAATGTATTTCGGGCGGAGGTTCTATGGCTTTGCTTCAGAGGCACAAATGGAACCAACAGTTGAGTCCGAAATTTTCAGAGCTCTTGATAAAGCCAGGCTTCTTGTTGGTGGTAGGAAGGAGTCTTGTTATTCAAGATGTGGTAGAACAGACAGGGGGGTCTCATCTGTTGGGCAAGTAATTGCTCTCTATTTACGGTCCAACCTTAAGGATCCCCCTAATGGTGAAAATGGCAATCATAATGCGGAAGAGCACCATGATGGAGAAATTGATTATGTGAGAGTAATAAATAGAGTTCTTCCCAATGACATTCGGGTTTTAGGCTGGTGTACTGTTCCTGTTGGCTTCAGTGCAAGATTTAGTTGTTTAAGAAGAAAGTATAAATATTTATTCTGGGGAGCAAACTTGAACATCTCGGCTATGGAGAGGGCCGGTAAGAAATTTGTAGGGGAACATGACTTCAGAAACTTCTGCAAAATGGATGCAGCAAATGTTCACAATTATAGGCGATGCATCATGTCATTTGACATTTCTCCTTGTGATGTGAGGATGGAAGGCAATCTATTATGGGCATTCAACATAGAAGGCAGTGCTTTCCTCTGGCATCAGGTCCGCTGCATGGTTGCTGTAGTATTCATGGTTGGGCAAGGTCTTGAAGATCCTGATGTGGTTGATATTCTACTGGATCCATTAAGAACACCAAGGAAGCCTCAATATGTTATGGCTTCAGAAAATCCTCTGGTTCTTCATTCTTGTGAATTTGAAGGTGTCAAATTTAGGTGTTCAACAGATGCTGGAGAAGCTCTACAGTTGCATTTATCAAATGAAATCCGCACATATGAGCTCCAAGCTGCAATATTCAAAGAAGCTCTTCTAAGCTGTTTGCCAATCACAGATGATGAGAGCCTTTCAGACAACAGAAAGATAAAGAAGAAAATTTGCCATGTTCCCCTATTGTCAAGACCAACTGAACCTTCTTACGAAGAGCGGCGTGCGAAGCTCAACTCATCCTCAAAGACATCCGGTGATGAAGGGGAGACGAGTCTAAAGAAAAAGCTGACAGCCCAACCACACCCCAACATCAACATGTACCATCATCATCATGAATCCAGGTGCATGAACTCCGACCAAATCATTTTTCTCACTTGCCCTGTCCCCCTCTCCTCTATGGTTTTATTAATGTTGAGAATGACTTGTGACGGTTCTGTGCTGTGTGTTTTTGTCCTGAACCATGTGAAAGCTTAA

Protein sequence

MKVSHRLRNTVFIGVSSFEKPKKLSGRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSSRLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPITDDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGDEGETSLKKKLTAQPHPNINMYHHHHESRCMNSDQIIFLTCPVPLSSMVLLMLRMTCDGSVLCVFVLNHVKA
Homology
BLAST of CmoCh15G005730 vs. ExPASy Swiss-Prot
Match: Q3SX07 (tRNA pseudouridine(38/39) synthase OS=Bos taurus OX=9913 GN=PUS3 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 3.2e-67
Identity = 139/291 (47.77%), Postives = 183/291 (62.89%), Query Frame = 0

Query: 156 KRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRG 215
           +R++ALK+ Y G  + GFAS+     T+E ++F AL K R LV  R+ S Y RCGRTD+G
Sbjct: 62  QRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTR-LVENRQTSNYHRCGRTDKG 121

Query: 216 VSSVGQVIALYLRSNLKDPPNGENGNH----NAEEHHDGEIDYVRVINRVLPNDIRVLGW 275
           VS+ GQVI+L LRS++   P G +  H    N       EI Y  ++NRVLP DIRVL W
Sbjct: 122 VSAFGQVISLDLRSHI---PKGRDSEHFNLKNEVNDVATEIRYTHILNRVLPPDIRVLAW 181

Query: 276 CTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAAN-VHNY 335
             V   FSARFSCL R Y+Y F  ANL+I  M  A +K+VG HDFRN CKMD AN V N+
Sbjct: 182 APVETSFSARFSCLERTYRYFFPRANLDIVTMNYAAQKYVGTHDFRNLCKMDVANGVINF 241

Query: 336 RRCIMSFDISPCDVRM------EGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPD 395
           +R I+S  +      +      E   L  F + G AFL+HQVRCM+AV+F++GQG+E P+
Sbjct: 242 QRTILSAQVQRVGQNLGEEGWQEPFQLCQFEVTGQAFLYHQVRCMMAVLFLIGQGMEKPE 301

Query: 396 VVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL 436
           V+D LL+  + P+KPQY MA E PLVL+ C+FE +K+    +  E    HL
Sbjct: 302 VIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENIKWIYDREVQEFNVTHL 348

BLAST of CmoCh15G005730 vs. ExPASy Swiss-Prot
Match: Q9JI38 (tRNA pseudouridine(38/39) synthase OS=Mus musculus OX=10090 GN=Pus3 PE=1 SV=2)

HSP 1 Score: 256.9 bits (655), Expect = 5.4e-67
Identity = 162/445 (36.40%), Postives = 240/445 (53.93%), Query Frame = 0

Query: 78  QSRLRLLQGRVKVLEEENSNLSSRLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKK 137
           ++++  L  RVK LE+E   L                       +K  +  ++KD + ++
Sbjct: 7   RNQIEKLLNRVKELEQEVERLK----------------------KKKEQANNIKDSSIRE 66

Query: 138 KAVEKTPGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLL 197
            ++               +R++ALK+ Y G  + GFAS+     T+E ++F AL K R L
Sbjct: 67  NSLGSGKAKRAFDFSAHGRRHVALKIAYLGWGYQGFASQENTSNTIEEKLFEALTKTR-L 126

Query: 198 VGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGN--HNAEEHHDGEIDYV 257
           V  R+ S Y RCGRTD+GVS+ GQVI+L LRS      + E+ N  H A++    EI Y 
Sbjct: 127 VESRQTSNYHRCGRTDKGVSAFGQVISLDLRSQFPTSRDSEDSNLKHEADDLAK-EIRYT 186

Query: 258 RVINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHD 317
            ++NRVLP DIRVL W  V   FSARFSCL R Y+Y F  A+L+I+ M  A +K+VG HD
Sbjct: 187 HILNRVLPADIRVLAWAPVEPSFSARFSCLERTYRYFFPRADLDIATMNYAAQKYVGTHD 246

Query: 318 FRNFCKMDAAN-VHNYRRCIMSFDI-----SPCDV-RMEGNLLWAFNIEGSAFLWHQVRC 377
           FRN CKMD AN V N++R I+   +     SP +  R E   L  F + G AFL+HQVRC
Sbjct: 247 FRNLCKMDVANGVINFQRTILCAQVQLVAQSPGEERRQEPFQLCQFEVIGQAFLYHQVRC 306

Query: 378 MVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAG 437
           M+A++F++GQG+E P+++D LL+  + P+KPQY MA E PLVL+ C+FE  K+    +  
Sbjct: 307 MMAILFLIGQGMEKPEIIDELLNIQKNPQKPQYSMAVEFPLVLYDCKFENTKWIYDHEVQ 366

Query: 438 EALQLHL-----SNEIRTYELQAAI--FKEALLSCLPITDDESLSDNRKIK--------- 493
           E    HL     ++ ++T+ L + +      +++C   T  +  ++ R I+         
Sbjct: 367 EFNVTHLQQLWANHAVKTHMLYSMLQGLDSVMVTCAAGTKMDEATEWRNIQPPVIKHTSA 426

BLAST of CmoCh15G005730 vs. ExPASy Swiss-Prot
Match: Q9BZE2 (tRNA pseudouridine(38/39) synthase OS=Homo sapiens OX=9606 GN=PUS3 PE=1 SV=3)

HSP 1 Score: 255.8 bits (652), Expect = 1.2e-66
Identity = 138/288 (47.92%), Postives = 187/288 (64.93%), Query Frame = 0

Query: 156 KRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRG 215
           +R++AL++ Y G  + GFAS+     T+E ++F AL K R LV  R+ S Y RCGRTD+G
Sbjct: 62  RRHVALRIAYMGWGYQGFASQENTNNTIEEKLFEALTKTR-LVESRQTSNYHRCGRTDKG 121

Query: 216 VSSVGQVIALYLRSNLKDPPNGENGNHNAEEHHDG-EIDYVRVINRVLPNDIRVLGWCTV 275
           VS+ GQVI+L LRS      + E+ N   E +    EI Y  ++NRVLP DIR+L W  V
Sbjct: 122 VSAFGQVISLDLRSQFPRGRDSEDFNVKEEANAAAEEIRYTHILNRVLPPDIRILAWAPV 181

Query: 276 PVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAAN-VHNYRRC 335
              FSARFSCL R Y+Y F  A+L+I  M+ A +K+VG HDFRN CKMD AN V N++R 
Sbjct: 182 EPSFSARFSCLERTYRYFFPRADLDIVTMDYAAQKYVGTHDFRNLCKMDVANGVINFQRT 241

Query: 336 IMSFDI-----SPCDVR-MEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVD 395
           I+S  +     SP + R  E   L  F + G AFL+HQVRCM+A++F++GQG+E P+++D
Sbjct: 242 ILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVRCMMAILFLIGQGMEKPEIID 301

Query: 396 ILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL 436
            LL+  + P+KPQY MA E PLVL+ C+FE VK+    +A E    HL
Sbjct: 302 ELLNIEKNPQKPQYSMAVEFPLVLYDCKFENVKWIYDQEAQEFNITHL 348

BLAST of CmoCh15G005730 vs. ExPASy Swiss-Prot
Match: P31115 (tRNA pseudouridine(38/39) synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DEG1 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 3.7e-60
Identity = 144/354 (40.68%), Postives = 208/354 (58.76%), Query Frame = 0

Query: 121 IEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQME 180
           IE ++K    +++ T+ KA +    ++       + R+IAL+  Y G  + G A + +  
Sbjct: 59  IEDNKKRKISQEEVTRSKAKKAPKKFD---FSKHNTRFIALRFAYLGWNYNGLAVQKEYT 118

Query: 181 --PTVESEIFRALDKARLLVG-GRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNG 240
             PTVE  I  A++K +L+     ++  +SRCGRTD+GVS++ QVI+L +RSNL D    
Sbjct: 119 PLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNLTDEEQR 178

Query: 241 ENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWC-TVPVGFSARFSCLRRKYKYLFWGA 300
           +  N +       EI YV V+N++LP+DIR+   C   P  F ARFSC+ R YKY+F G 
Sbjct: 179 DPTNDSR------EIPYVHVLNQLLPDDIRISAVCLRPPPNFDARFSCVHRHYKYIFNGK 238

Query: 301 NLNISAMERAGKKFVGEHDFRNFCKMDAA-NVHNYRRCIMSFDISPCDVRMEGNLLWAFN 360
           NLNI  M +A   FVGE DFRNFCK+D +  + N++R I+S  I P          + F+
Sbjct: 239 NLNIEKMSKAASYFVGERDFRNFCKLDGSKQITNFKRTIISSKILPL-----SETFYCFD 298

Query: 361 IEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCE 420
           + GSAFLWHQVRCM+A++F+VGQ LE P++V  L D  +TP++P Y MA++ PL+L+ C+
Sbjct: 299 LVGSAFLWHQVRCMMAILFLVGQSLEVPEIVLRLTDIEKTPQRPVYEMANDIPLLLYDCK 358

Query: 421 F-------------EGVKFRCSTDAGEALQLHLSNEIRTYELQAA---IFKEAL 454
           F             + +KF   T A EAL LH       YEL+AA   IFK+ L
Sbjct: 359 FPEMDWQEPTVDDYKAIKF---TTATEALTLH-------YELKAAVCNIFKDVL 388

BLAST of CmoCh15G005730 vs. ExPASy Swiss-Prot
Match: Q9UTB3 (Putative tRNA pseudouridine synthase C25B8.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC25B8.05 PE=3 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 8.6e-57
Identity = 120/278 (43.17%), Postives = 173/278 (62.23%), Query Frame = 0

Query: 158 YIALKVMYFGRRFYGFASEAQME--PTVESEIFRALDKARLLVGGRKESC-YSRCGRTDR 217
           ++A K  Y G  + G A + +    PTVE ++F AL +A L+      SC +SRCGRTD+
Sbjct: 93  FVAFKFAYCGWNYNGLAYQLEPTPLPTVEGKVFEALLRAHLITD--PSSCSFSRCGRTDK 152

Query: 218 GVSSVGQVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTV 277
           GVS++GQVI+L +R + + P                 I Y  V+NR+LP DIR+ G+   
Sbjct: 153 GVSAMGQVISLNVRHSERRP-----------------IIYCDVLNRLLPADIRITGYAFP 212

Query: 278 PVGFSARFSCLRRKYKYLFW----GANLNISAMERAGKKFVGEHDFRNFCKMDAA-NVHN 337
           P GF ARFSC +R YKYLF     G +L+I  M  A   ++GEHDFRNFCK+DA+  + N
Sbjct: 213 PEGFDARFSCKQRHYKYLFTKDYPGGSLDIDRMNEAAALYLGEHDFRNFCKIDASKQITN 272

Query: 338 YRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDIL 397
           Y R I+S  +   D       L+AF+++G+AFLWHQVRCM+A++F++GQ LE   +++ L
Sbjct: 273 YHRRILSSKVICID---PSTGLYAFDLQGTAFLWHQVRCMMAILFLIGQKLEPASLINDL 332

Query: 398 LDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDA 428
           LD  + P KP Y MASE PL+L+ C+F+ +++   TD+
Sbjct: 333 LDIKKVPTKPIYDMASEYPLILYDCDFDNIEWTLPTDS 348

BLAST of CmoCh15G005730 vs. ExPASy TrEMBL
Match: A0A6J1FEX0 (tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444871 PE=3 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.7e-271
Identity = 462/465 (99.35%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 460
           YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 420

Query: 461 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK+S +
Sbjct: 421 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKSSSN 465

BLAST of CmoCh15G005730 vs. ExPASy TrEMBL
Match: A0A6J1JQK1 (tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 3.3e-261
Identity = 444/465 (95.48%), Postives = 458/465 (98.49%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQS+LRLLQGRVKVLEEENSNLSS
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKVLEEENSNLSS 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLA+C CSERRMNFDDGSSSIEKS+KYGSLKDKTT+KKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTD+GVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHN++EHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDP+RTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 460
           YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL+NEIRTYELQAAIFKEALLSCLPIT
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLTNEIRTYELQAAIFKEALLSCLPIT 420

Query: 461 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           +DES SDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK+S +
Sbjct: 421 NDESFSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKSSSN 465

BLAST of CmoCh15G005730 vs. ExPASy TrEMBL
Match: A0A6J1JWD4 (tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488950 PE=3 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.6e-260
Identity = 443/465 (95.27%), Postives = 457/465 (98.28%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQS+LRLLQGRVK LEEENSNLSS
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKELEEENSNLSS 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLA+C CSERRMNFDDGSSSIEKS+KYGSLKDKTT+KKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTD+GVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHN++EHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDP+RTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 460
           YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL+NEIRTYELQAAIFKEALLSCLPIT
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLTNEIRTYELQAAIFKEALLSCLPIT 420

Query: 461 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           +DES SDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK+S +
Sbjct: 421 NDESFSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKSSSN 465

BLAST of CmoCh15G005730 vs. ExPASy TrEMBL
Match: A0A6J1FKL6 (tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444871 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 9.7e-229
Identity = 391/394 (99.24%), Postives = 393/394 (99.75%), Query Frame = 0

Query: 112 MNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIALKVMYFGRRFY 171
           MNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIALKVMYFGRRFY
Sbjct: 1   MNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIALKVMYFGRRFY 60

Query: 172 GFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNL 231
           GFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNL
Sbjct: 61  GFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNL 120

Query: 232 KDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKY 291
           KDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKY
Sbjct: 121 KDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKY 180

Query: 292 LFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDISPCDVRMEGNLL 351
           LFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDISPCDVRMEGNLL
Sbjct: 181 LFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDISPCDVRMEGNLL 240

Query: 352 WAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVL 411
           WAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVL
Sbjct: 241 WAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVL 300

Query: 412 HSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPITDDESLSDNRKI 471
           HSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPITDDESLSDNRKI
Sbjct: 301 HSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPITDDESLSDNRKI 360

Query: 472 KKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           KKKICHVPLLSRPTEPSYEERRAKLNSSSK+S +
Sbjct: 361 KKKICHVPLLSRPTEPSYEERRAKLNSSSKSSSN 394

BLAST of CmoCh15G005730 vs. ExPASy TrEMBL
Match: A0A6J1FEX6 (tRNA pseudouridine(38/39) synthase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111444871 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.6e-226
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCST 426
           YVMASENPLVLHSCEFEGVKFRCST
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCST 385

BLAST of CmoCh15G005730 vs. NCBI nr
Match: KAG6578858.1 (putative F-actin-capping protein subunit beta, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 957.6 bits (2474), Expect = 4.9e-275
Identity = 467/479 (97.49%), Postives = 476/479 (99.37%), Query Frame = 0

Query: 23  KLSGRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLR 82
           KLSGRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LR
Sbjct: 2   KLSGRLNSGAVFLDVQGLMDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLR 61

Query: 83  LLQGRVKVLEEENSNLSSRLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEK 142
           LLQGRVK LEEENSNLSSRLA+CCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKAVEK
Sbjct: 62  LLQGRVKELEEENSNLSSRLASCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEK 121

Query: 143 TPGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRK 202
           TPGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRK
Sbjct: 122 TPGYNTRILDHCSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRK 181

Query: 203 ESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVL 262
           ESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHN+EEHHDGEIDYVRV+NRVL
Sbjct: 182 ESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVL 241

Query: 263 PNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKM 322
           PNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKM
Sbjct: 242 PNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKM 301

Query: 323 DAANVHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLED 382
           DAANVHNYRRCIMSFDISPCDVRMEGN LWAFNIEGSAFLWHQVRCMVAVVFMVGQGLED
Sbjct: 302 DAANVHNYRRCIMSFDISPCDVRMEGNPLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLED 361

Query: 383 PDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTY 442
           PDVVDILLDP+RTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL+NEIRTY
Sbjct: 362 PDVVDILLDPVRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLTNEIRTY 421

Query: 443 ELQAAIFKEALLSCLPITDDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK 502
           ELQAAIFKEALLSCLPIT+DES SDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK
Sbjct: 422 ELQAAIFKEALLSCLPITNDESFSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK 480

BLAST of CmoCh15G005730 vs. NCBI nr
Match: XP_022938732.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata] >XP_022938734.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata])

HSP 1 Score: 944.1 bits (2439), Expect = 5.6e-271
Identity = 462/465 (99.35%), Postives = 464/465 (99.78%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 460
           YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 420

Query: 461 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK+S +
Sbjct: 421 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKSSSN 465

BLAST of CmoCh15G005730 vs. NCBI nr
Match: XP_023551060.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551061.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.9 bits (2389), Expect = 3.5e-265
Identity = 449/465 (96.56%), Postives = 460/465 (98.92%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQS+LRLLQGRVK LEEENSNLS+
Sbjct: 1   MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSQLRLLQGRVKELEEENSNLST 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLATCCCSERRMNFDDGSSSIEKS+KYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLATCCCSERRMNFDDGSSSIEKSKKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRAL+KARLLVGGRKESCYSRCGRTDRGVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALEKARLLVGGRKESCYSRCGRTDRGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHN+EEHHDGEIDYVRV+NRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNSEEHHDGEIDYVRVMNRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVV+MVGQGLEDPDVVDILLDPLRTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVYMVGQGLEDPDVVDILLDPLRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 460
           YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL+NEIRTYELQAAIFKEALLSCLPIT
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLTNEIRTYELQAAIFKEALLSCLPIT 420

Query: 461 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           +DES  DNRKIKKKICHVPL SRPTEPSYEERRAKLNSSSK+S +
Sbjct: 421 NDESFPDNRKIKKKICHVPLFSRPTEPSYEERRAKLNSSSKSSSN 465

BLAST of CmoCh15G005730 vs. NCBI nr
Match: XP_022992677.1 (tRNA pseudouridine(38/39) synthase isoform X2 [Cucurbita maxima])

HSP 1 Score: 910.6 bits (2352), Expect = 6.8e-261
Identity = 444/465 (95.48%), Postives = 458/465 (98.49%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQS+LRLLQGRVKVLEEENSNLSS
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKVLEEENSNLSS 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLA+C CSERRMNFDDGSSSIEKS+KYGSLKDKTT+KKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTD+GVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHN++EHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDP+RTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 460
           YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL+NEIRTYELQAAIFKEALLSCLPIT
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLTNEIRTYELQAAIFKEALLSCLPIT 420

Query: 461 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           +DES SDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK+S +
Sbjct: 421 NDESFSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKSSSN 465

BLAST of CmoCh15G005730 vs. NCBI nr
Match: XP_022992675.1 (tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita maxima] >XP_022992676.1 tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita maxima])

HSP 1 Score: 908.3 bits (2346), Expect = 3.4e-260
Identity = 443/465 (95.27%), Postives = 457/465 (98.28%), Query Frame = 0

Query: 41  MDGAEHSLDYRGGTGAMETTSGTDEFQPFDGNLIGNLQSRLRLLQGRVKVLEEENSNLSS 100
           MDGAEHSLD+RG TGAMETTSGTDEFQPFD NLI NLQS+LRLLQGRVK LEEENSNLSS
Sbjct: 1   MDGAEHSLDHRGATGAMETTSGTDEFQPFDRNLIENLQSQLRLLQGRVKELEEENSNLSS 60

Query: 101 RLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKKKAVEKTPGYNTRILDHCSKRYIA 160
           RLA+C CSERRMNFDDGSSSIEKS+KYGSLKDKTT+KKAVEKTPGYNTRILDHCSKRYIA
Sbjct: 61  RLASCYCSERRMNFDDGSSSIEKSKKYGSLKDKTTEKKAVEKTPGYNTRILDHCSKRYIA 120

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTD+GVSSVG
Sbjct: 121 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDKGVSSVG 180

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QVIALYLRSNLKDPPNGENGNHN++EHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA
Sbjct: 181 QVIALYLRSNLKDPPNGENGNHNSKEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 240

Query: 281 RFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 340
           RFSCLRR+YKYLFWGANLNISAME AGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS
Sbjct: 241 RFSCLRRRYKYLFWGANLNISAMESAGKKFVGEHDFRNFCKMDAANVHNYRRCIMSFDIS 300

Query: 341 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPLRTPRKPQ 400
           PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDP+RTPRKPQ
Sbjct: 301 PCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDPDVVDILLDPVRTPRKPQ 360

Query: 401 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLSNEIRTYELQAAIFKEALLSCLPIT 460
           YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHL+NEIRTYELQAAIFKEALLSCLPIT
Sbjct: 361 YVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLTNEIRTYELQAAIFKEALLSCLPIT 420

Query: 461 DDESLSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKTSGD 506
           +DES SDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSK+S +
Sbjct: 421 NDESFSDNRKIKKKICHVPLLSRPTEPSYEERRAKLNSSSKSSSN 465

BLAST of CmoCh15G005730 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 476.9 bits (1226), Expect = 2.4e-134
Identity = 236/425 (55.53%), Postives = 299/425 (70.35%), Query Frame = 0

Query: 78  QSRLRLLQGRVKVLEEENSNLSSRLATCCCSERRMNFDDGSSSIEKSRKYGSLKDKTTKK 137
           ++ L  L+ RVK LE EN+ L S++++C C +  +  D             SL D ++  
Sbjct: 22  ETELVFLRNRVKELEVENAKLLSQVSSCQCQQMEVKHD------------RSLSDSSSLV 81

Query: 138 KAVEKTPGYNTRILDH-CSKRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARL 197
           +      G    I  H  SKRY+ALK+MYFG+RFYGF++EAQMEP++ESE+F+AL++ RL
Sbjct: 82  RRRRVRKGDKNSIPSHLISKRYVALKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRL 141

Query: 198 LVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVR 257
           LVG +K+SCYSRCGRTD+GVSS GQVIAL+LRS LK PP       N       E DYVR
Sbjct: 142 LVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVR 201

Query: 258 VINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYLFWGANLNISAMERAGKKFVGEHDF 317
           V+NR LP+DIRV+GW   P+ F ARFSC  R+YKY FW  NLN+SAM+ AGKKF+GEHDF
Sbjct: 202 VLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWRQNLNLSAMDFAGKKFIGEHDF 261

Query: 318 RNFCKMDAANVHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMV 377
           RNFCKMD ANVH Y R +  F++SPC    EG+ L  F + GSAFLWHQ+RCMVAV+FM+
Sbjct: 262 RNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMI 321

Query: 378 GQGLEDPDVVDILLDPLRTPRKPQYVMASENPLVLHSCEFEGVKFRCSTDAGEALQLHLS 437
           GQG+E  DV+D LLD  +TPRKPQY++ASE PLVL +CEFE V F CS  A E+L+ H  
Sbjct: 322 GQGVESVDVIDTLLDTKKTPRKPQYLLASEIPLVLRTCEFENVDFICSPGAAESLRSHFK 381

Query: 438 NEIRTYELQAAIFKEALLSCLPI-----TDDESLSDNRKIKKKICHVPLLSRPTEPSYEE 497
           NE  TY+L++ I++EAL +CLP+     T++ S +   K KK+  HVPLLSRPTEPSYEE
Sbjct: 382 NESLTYQLESVIYQEALRNCLPLSNNVSTEESSCNGVEKKKKRAEHVPLLSRPTEPSYEE 434

BLAST of CmoCh15G005730 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 90.1 bits (222), Expect = 6.2e-18
Identity = 84/276 (30.43%), Postives = 124/276 (44.93%), Query Frame = 0

Query: 161 LKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVG 220
           L + Y G RF G+  + +  PT++S + +AL +   L  GRKE      GRTD GV + G
Sbjct: 45  LVIAYDGTRFAGWQYQ-ESPPTIQSMLEKALIQITEL--GRKELQLIGAGRTDAGVHAWG 104

Query: 221 QVIALYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSA 280
           QV       N            + +  H         +N +LP DIRV         F A
Sbjct: 105 QVAHFVTPFNYT----------SLDSFH-------AALNGLLPKDIRVRELSAAVPEFHA 164

Query: 281 RFSCLRRKYKYLFW----------------GANLNISAMERAGKKFVGEHDFRNFCKMDA 340
           RFS   + Y+Y  +                   LN S M  A   FVG+HDF  F     
Sbjct: 165 RFSASSKVYRYQIYNDTFMDPFQRHWAYHCAYKLNASKMREAANLFVGKHDFSAFANATR 224

Query: 341 AN-VHNYRRCIMSFDISPCDVRMEGNLLWAFNIEGSAFLWHQVRCMVAVVFMVGQGLEDP 400
            + V +  + I  FD+    ++M G+LL    +EGS FL+ QVR MVA++  +G+   D 
Sbjct: 225 EDGVPDPLKTISRFDV----IQM-GSLL-QLEVEGSGFLYRQVRNMVALLIQIGKEALDS 284

Query: 401 DVVDILLD--PLRTPRKPQYVMASENPLVLHSCEFE 418
           D+V ++L+    R   K   + AS + L L S +++
Sbjct: 285 DIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYK 294

BLAST of CmoCh15G005730 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 6.4e-07
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 0

Query: 179 MEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGE 238
           +E  +E  +F A      + G  K   ++R  RTD+GVS+VGQV++      + DP    
Sbjct: 47  IEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVS---GRFIVDP---- 106

Query: 239 NGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYL 293
                        + +V  +N  LPN IR+ G+  V   FS++  C RR+Y YL
Sbjct: 107 -------------LGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYL 140

BLAST of CmoCh15G005730 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 6.4e-07
Identity = 42/143 (29.37%), Postives = 66/143 (46.15%), Query Frame = 0

Query: 156 KRYIALKVMYFGRRFYGFASEAQMEPTVESEIFRALDKARLLVGGRKESC----YSRCGR 215
           +R +A+   + G  + G       + T+E E+  AL  A  +    +       ++R  R
Sbjct: 57  RRKVAIVFAFCGVGYQGMQKNPGAK-TIEGELEEALFHAGAVPDADRNKPRNYEWARSAR 116

Query: 216 TDRGVSSVGQVIA--LYLRSNLKDPPNGENGNHNAEEHHDGEIDYVRVINRVLPNDIRVL 275
           TD+GVS+VGQV++   Y+     DPP                  +V  +N  LP+ IRV 
Sbjct: 117 TDKGVSAVGQVVSGRFYV-----DPPG-----------------FVERLNSKLPDQIRVF 176

Query: 276 GWCTVPVGFSARFSCLRRKYKYL 293
           G+  V   FS++  C RR+Y YL
Sbjct: 177 GYKRVAPSFSSKKFCDRRRYVYL 176

BLAST of CmoCh15G005730 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 6.4e-07
Identity = 36/114 (31.58%), Postives = 55/114 (48.25%), Query Frame = 0

Query: 179 MEPTVESEIFRALDKARLLVGGRKESCYSRCGRTDRGVSSVGQVIALYLRSNLKDPPNGE 238
           +E  +E  +F A      + G  K   ++R  RTD+GVS+VGQV++      + DP    
Sbjct: 47  IEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVS---GRFIVDP---- 106

Query: 239 NGNHNAEEHHDGEIDYVRVINRVLPNDIRVLGWCTVPVGFSARFSCLRRKYKYL 293
                        + +V  +N  LPN IR+ G+  V   FS++  C RR+Y YL
Sbjct: 107 -------------LGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYL 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3SX073.2e-6747.77tRNA pseudouridine(38/39) synthase OS=Bos taurus OX=9913 GN=PUS3 PE=2 SV=1[more]
Q9JI385.4e-6736.40tRNA pseudouridine(38/39) synthase OS=Mus musculus OX=10090 GN=Pus3 PE=1 SV=2[more]
Q9BZE21.2e-6647.92tRNA pseudouridine(38/39) synthase OS=Homo sapiens OX=9606 GN=PUS3 PE=1 SV=3[more]
P311153.7e-6040.68tRNA pseudouridine(38/39) synthase OS=Saccharomyces cerevisiae (strain ATCC 2045... [more]
Q9UTB38.6e-5743.17Putative tRNA pseudouridine synthase C25B8.05 OS=Schizosaccharomyces pombe (stra... [more]
Match NameE-valueIdentityDescription
A0A6J1FEX02.7e-27199.35tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JQK13.3e-26195.48tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1JWD41.6e-26095.27tRNA pseudouridine(38/39) synthase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FKL69.7e-22999.24tRNA pseudouridine(38/39) synthase isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1FEX61.6e-226100.00tRNA pseudouridine(38/39) synthase isoform X3 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
KAG6578858.14.9e-27597.49putative F-actin-capping protein subunit beta, partial [Cucurbita argyrosperma s... [more]
XP_022938732.15.6e-27199.35tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita moschata] >XP_022938734... [more]
XP_023551060.13.5e-26596.56tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_022992677.16.8e-26195.48tRNA pseudouridine(38/39) synthase isoform X2 [Cucurbita maxima][more]
XP_022992675.13.4e-26095.27tRNA pseudouridine(38/39) synthase isoform X1 [Cucurbita maxima] >XP_022992676.1... [more]
Match NameE-valueIdentityDescription
AT1G34150.12.4e-13455.53Pseudouridine synthase family protein [more]
AT3G06950.16.2e-1830.43Pseudouridine synthase family protein [more]
AT1G76120.16.4e-0731.58Pseudouridine synthase family protein [more]
AT1G20370.16.4e-0729.37Pseudouridine synthase family protein [more]
AT1G76120.26.4e-0731.58Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 74..101
NoneNo IPR availablePIRSRPIRSR001430-2PIRSR001430-2coord: 202..411
e-value: 7.9E-34
score: 115.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 486..511
NoneNo IPR availablePANTHERPTHR11142:SF5TRNA PSEUDOURIDINE(38/39) SYNTHASEcoord: 78..499
IPR020097Pseudouridine synthase I, TruA, alpha/beta domainPFAMPF01416PseudoU_synth_1coord: 307..417
e-value: 1.2E-28
score: 99.7
IPR020095Pseudouridine synthase I, TruA, C-terminalGENE3D3.30.70.660coord: 285..459
e-value: 1.4E-40
score: 141.0
IPR020094Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminalGENE3D3.30.70.580coord: 148..284
e-value: 6.4E-32
score: 112.1
IPR001406Pseudouridine synthase I, TruATIGRFAMTIGR00071TIGR00071coord: 158..403
e-value: 1.1E-50
score: 170.4
IPR001406Pseudouridine synthase I, TruAPANTHERPTHR11142PSEUDOURIDYLATE SYNTHASEcoord: 78..499
IPR001406Pseudouridine synthase I, TruAHAMAPMF_00171TruAcoord: 157..416
score: 23.408983
IPR041707Pseudouridine synthase Pus3-likeCDDcd02569PseudoU_synth_ScPus3coord: 161..416
e-value: 2.47704E-134
score: 389.723
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 156..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh15G005730.1CmoCh15G005730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0008033 tRNA processing
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding