Homology
BLAST of CmoCh15G000190 vs. ExPASy Swiss-Prot
Match:
F4IYF8 (Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760 PE=2 SV=1)
HSP 1 Score: 916.8 bits (2368), Expect = 2.1e-265
Identity = 538/1001 (53.75%), Postives = 666/1001 (66.53%), Query Frame = 0
Query: 5 AAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHHLF-V 64
AAE S ITLGGKGSSL+S+SVY +A G + VRIDSS++ER ++ N SIK F +
Sbjct: 2 AAERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGI 61
Query: 65 PDFLTRDEARASLVVLLNKLIIST-----SSGIRSVIPILIAETLNSNPETLQFEPLDVT 124
P LT +E RASL VLLNKLI+ST SS RSV+P+ I E LN E+L+ +DVT
Sbjct: 62 PQGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVT 121
Query: 125 PEELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFA 184
E V E+SC L GIC+++DH+S LS I D+VA++SCE ++AD+++FSLLDSGDG
Sbjct: 122 EGENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNG 181
Query: 185 SKEEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKI 244
K+ +G+A D+KV LNG K GK+E IS IP HG R K VH+ R ELNS VK
Sbjct: 182 DKDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKG 241
Query: 245 GKGGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRE 304
GK GS ++G +A+ T LL + +LG CS R KL DEN+K L+ ++
Sbjct: 242 GK-TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLS---EKI 301
Query: 305 CPSSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGEL-IG 364
C +E+LK Y+L L+E Y F H +N VW+IV EA+AAF + GGEL +
Sbjct: 302 CVENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQ 361
Query: 365 KGQDVD-INGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKL-----QGKGGGLGSLENL 424
K D D +K KKNE KKAV+GKG+S+V+Q IKD+L G + SL
Sbjct: 362 KSGDADKEESKTDKKKKKNE--KKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQC 421
Query: 425 VKDLLSFLDPKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSI 484
+ +L+ +P+ FD+LL K+KEIVESNE+RRLPKLP KGTRDFAKEQMA+R+KAFSI
Sbjct: 422 GEQILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLP--KGTRDFAKEQMAVREKAFSI 481
Query: 485 IEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYV 544
I+ VF+RHGATALDTP FELRETLMGKYGEDSKLVYD+ADQGGELCSLRYDLTVPFARYV
Sbjct: 482 IQNVFKRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYV 541
Query: 545 AMNGITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDE 604
AMNGITSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDE
Sbjct: 542 AMNGITSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDE 601
Query: 605 LNIGQKGLAVLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVP 664
L IG +Y+ +KLNHR+LLDGMLEICGVP
Sbjct: 602 LEIG--------DYE--------------------------VKLNHRKLLDGMLEICGVP 661
Query: 665 SDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQE 724
+KFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA+RIG+FVKE+G PLELLSKL+QE
Sbjct: 662 PEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGAPLELLSKLRQE 721
Query: 725 QSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ 784
S L N+ S +AL+EL I+F AL++S C +++VFDLSLARGLDYYTGVI+EAV G
Sbjct: 722 GSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSLARGLDYYTGVIFEAVCIGAE- 781
Query: 785 RIFLDNSTDSDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIA 844
+GSI
Sbjct: 782 -------------------------------------------------------VGSIG 841
Query: 845 AGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQT 904
AGGRYDNLIGMFG+KQVPAVG+SLGIERVF IME+L + Q Q+
Sbjct: 842 AGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIMEELNEKQKQV----------------- 883
Query: 905 TRATKTEVLVSILGDD-LTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVF 964
R T+T+VLVSI+ D+ L +AAEL S++W AK++AE+LV+KR KH +RAKE+ IPW+V
Sbjct: 902 IRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKESGIPWMVM 883
Query: 965 LGEREASEGIVKLKNV----ETFEEVTVSRSNIIDELKKRL 988
+GE+E S V LK + E E+ T +R ++ELKK L
Sbjct: 962 VGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of CmoCh15G000190 vs. ExPASy Swiss-Prot
Match:
P93422 (Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150900 PE=2 SV=2)
HSP 1 Score: 698.7 bits (1802), Expect = 9.2e-200
Identity = 416/868 (47.93%), Postives = 535/868 (61.64%), Query Frame = 0
Query: 139 LDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASKEEVGIANDMKVFLNGSKL 198
+D ++ L +ADAVA++SCEA+R DV+AF + SGDG ++K+E +A D+K+ L GSKL
Sbjct: 36 IDCCASPLVRVADAVAALSCEAARGDVAAFDVPTSGDGLSAKDEADVAADVKMLLFGSKL 95
Query: 199 VGK---IESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGKGGG-SLSSGTEDAVR 258
VG ++ + + +P +G RE + +H+++R+ELN+ VK+GK G E+A+
Sbjct: 96 VGAAGGADAASFTKVPTVNGIFREAVRALHARVRIELNAPVKLGKRDAVQTGEGKEEALV 155
Query: 259 TVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECPSSESLKREYRLVSE 318
+ A + + K S+ R +L + D ++ L ++ + LK ++ +
Sbjct: 156 ALATQLARPVQAMLKLSVARARLCVARIDDAELRKKLTDGVE-----IDDLK---GMLGK 215
Query: 319 LGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQDVDINGA------- 378
+ +D V + V I+AWEA A IE I K Q + N A
Sbjct: 216 VTIDSDAVSVLRGVYNSLLKFRDILAWEAAVAMAVIEMDSSIEKPQACEENEAGSSTENP 275
Query: 379 ---NEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLV-----KDLLSFL 438
EK + KKK LGKG+S V+ L++D L G + S+ + + +L
Sbjct: 276 HASGEKPKGDKKSKKKKTLGKGTSAVLMLLRD-LVTNGKEVLSVNSALLAEWGTELSLLF 335
Query: 439 DPKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERH 498
DPK +L+ K+KEIVE+NE RRLPK+P KGTRDF KEQMAIR+ AFSII GVF+ H
Sbjct: 336 DPKCPRLVSLVDKVKEIVETNEVRRLPKIP--KGTRDFGKEQMAIREHAFSIITGVFKMH 395
Query: 499 GATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 558
GA +LDTP FELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMN I+S
Sbjct: 396 GAVSLDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNNISSL 455
Query: 559 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGL 618
KRYQIAKVYRRDNPSKGR+REFYQCDFDIAG YE M PDFEVIK+LTELLD+L+IG
Sbjct: 456 KRYQIAKVYRRDNPSKGRYREFYQCDFDIAGVYETMEPDFEVIKVLTELLDQLDIG---- 515
Query: 619 AVLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTIC 678
Y+ IKLNHR+LLDGMLEICGVP +KFRT+C
Sbjct: 516 ----TYE--------------------------IKLNHRKLLDGMLEICGVPPEKFRTVC 575
Query: 679 SSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNK 738
SSIDKLDKQ+F+Q+K+E+V+EKG++ E A++IGD VK RG PLE+L +L++E S + N
Sbjct: 576 SSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLVKTRGPPLEVLLELRKEGSKFMGNA 635
Query: 739 GSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNST 798
GS ALNELEILF AL+K+N + K+VFDLSLARGLDYYTGVIYEAVFKG TQ
Sbjct: 636 GSVTALNELEILFKALDKANAIGKIVFDLSLARGLDYYTGVIYEAVFKGTTQ-------- 695
Query: 799 DSDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNL 858
+GSIAAGGRYDNL
Sbjct: 696 -----------------------------------------------VGSIAAGGRYDNL 755
Query: 859 IGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEV 918
+GMF KQVPAVGVSLGIERVF IMEQ ++NQ+ RAT+TEV
Sbjct: 756 VGMFSGKQVPAVGVSLGIERVFAIMEQQEMEKNQI------------------RATETEV 785
Query: 919 LVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEG 978
LVSI+G DL AAEL SE+W+A + AEF + R+ H+ A ++ IPW+V +GE E S G
Sbjct: 816 LVSIIGKDLILAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSGIPWMVLVGESEISSG 785
Query: 979 IVKLKNVETFEEVTVSRSNIIDELKKRL 988
VKLKN+ +E V R+ LK++L
Sbjct: 876 KVKLKNLAASQEEEVDRTEFAQVLKQKL 785
BLAST of CmoCh15G000190 vs. ExPASy Swiss-Prot
Match:
E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)
HSP 1 Score: 433.3 bits (1113), Expect = 7.2e-120
Identity = 257/564 (45.57%), Postives = 347/564 (61.52%), Query Frame = 0
Query: 430 LRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAF 489
L ++K + +E + + L +KGTRD+ +QMAIR+K F+II F+RHGA +D+P F
Sbjct: 38 LLQLKAQLGGDEGKHVFVLKTAKGTRDYNPKQMAIREKVFNIIINCFKRHGAETIDSPVF 97
Query: 490 ELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYR 549
EL+ETL GKYGEDSKL+YDL DQGGEL SLRYDLTVPFARY+AMN IT+ KRY IAKVYR
Sbjct: 98 ELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYR 157
Query: 550 RDNP--SKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLAVLNNYDE 609
RDNP ++GR+REFYQCDFDIAGQY+ M PD E +K++ E+L EL++G
Sbjct: 158 RDNPAMTRGRYREFYQCDFDIAGQYDAMIPDAECLKLVYEILSELDLGD----------- 217
Query: 610 SASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDK 669
RIK+N RR+LDGM ICGVP +KFRTICS++DKLDK
Sbjct: 218 -----------------------FRIKVNDRRILDGMFAICGVPDEKFRTICSTVDKLDK 277
Query: 670 QSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNE 729
+++++K+EMV EKGL+ E+A+RI D+V +G +L +L Q+ L Q+K + + +
Sbjct: 278 LAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGK-DLAERLLQDPK-LSQSKQACAGITD 337
Query: 730 LEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTDSDWEDFV 789
+++LFS LE DKVVFDLSLARGLDYYTGVIYEA+ TQ ST ++
Sbjct: 338 MKLLFSYLELFQITDKVVFDLSLARGLDYYTGVIYEAIL---TQANPAPASTPAEQNG-- 397
Query: 790 VLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLIGMFGSK- 849
ED + +GS+A GGRYD L+GMF K
Sbjct: 398 -------------------------------AEDAGVS-VGSVAGGGRYDGLVGMFDPKG 457
Query: 850 -QVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVLV-SIL 909
+VP VGVS+GIERVF IMEQ K + S + R T+T+VLV S
Sbjct: 458 RKVPCVGVSIGIERVFSIMEQ---------KAELSS--------EKVRTTETQVLVASAQ 511
Query: 910 GDDLTQAAELASEIWSAKVHAEFLV--NKRVMKHIDRAKEARIPWIVFLGEREASEGIVK 969
+ L + +L +E+W+A + AE L N +++ + ++ IP + LGE+E +G+VK
Sbjct: 518 KNLLEERLKLTAELWNAGIKAEVLYKKNPKLLSQLQHCEDTGIPLVAILGEQELKDGVVK 511
Query: 970 LKNVETFEEVTVSRSNIIDELKKR 987
L+NV + EEV V R+ ++DE+KKR
Sbjct: 578 LRNVASREEVDVPRAELVDEVKKR 511
BLAST of CmoCh15G000190 vs. ExPASy Swiss-Prot
Match:
Q61035 (Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2)
HSP 1 Score: 423.7 bits (1088), Expect = 5.7e-117
Identity = 248/564 (43.97%), Postives = 339/564 (60.11%), Query Frame = 0
Query: 430 LRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAF 489
L K+K + +E ++ L KGTRD++ QMA+R+K F +I F+RHGA +DTP F
Sbjct: 38 LLKLKAQLGQDEGKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVF 97
Query: 490 ELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYR 549
EL+ETL GKYGEDSKL+YDL DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYR
Sbjct: 98 ELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYR 157
Query: 550 RDNP--SKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLAVLNNYDE 609
RDNP ++GR+REFYQCDFDIAGQ++ M PD E +KI+ E+L L IG
Sbjct: 158 RDNPAMTRGRYREFYQCDFDIAGQFDPMIPDAECLKIMCEILSSLQIG------------ 217
Query: 610 SASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDK 669
N + +K+N RR+LDGM +CGVP KFRTICSS+DKLDK
Sbjct: 218 --------NFL--------------VKVNDRRILDGMFAVCGVPDSKFRTICSSVDKLDK 277
Query: 670 QSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNE 729
S++++K EMV EKGL E+A+RIGD+V++ G + L+ +L Q+ L QNK + + L +
Sbjct: 278 VSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDPK-LSQNKQAVEGLGD 337
Query: 730 LEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTDSDWEDFV 789
L++LF L DK+ FDLSLARGLDYYTGVIYEAV
Sbjct: 338 LKLLFEYLILFGIDDKISFDLSLARGLDYYTGVIYEAV---------------------- 397
Query: 790 VLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLIGMFGSK- 849
L+ +P E+ + +GSIAAGGRYD L+GMF K
Sbjct: 398 -----------------------LLQMPTQAGEEPL--GVGSIAAGGRYDGLVGMFDPKG 457
Query: 850 -QVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVLV-SIL 909
+VP VG+S+G+ER+F I+EQ L+ + R T+T+VLV S
Sbjct: 458 RKVPCVGLSIGVERIFSIVEQRLEASE-----------------EKVRTTETQVLVASAQ 501
Query: 910 GDDLTQAAELASEIWSAKVHAEFLV--NKRVMKHIDRAKEARIPWIVFLGEREASEGIVK 969
L + +L SE+W A + AE L N +++ + +EA IP + +GE+E +G++K
Sbjct: 518 KKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIK 501
Query: 970 LKNVETFEEVTVSRSNIIDELKKR 987
L++V + EEV V R ++++E+++R
Sbjct: 578 LRSVASREEVDVRREDLVEEIRRR 501
BLAST of CmoCh15G000190 vs. ExPASy Swiss-Prot
Match:
P70076 (Histidine--tRNA ligase, cytoplasmic OS=Takifugu rubripes OX=31033 GN=hars1 PE=3 SV=1)
HSP 1 Score: 422.9 bits (1086), Expect = 9.7e-117
Identity = 244/565 (43.19%), Postives = 339/565 (60.00%), Query Frame = 0
Query: 430 LRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAF 489
L ++K + ++ + L +KGTRD+ +QMAIR+K F+ I F+RHGA +DTP F
Sbjct: 43 LLELKAHLGGDDGKHQFVLKTAKGTRDYNPKQMAIREKVFNTIVSCFKRHGAETIDTPVF 102
Query: 490 ELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYR 549
EL+ETL GKYGEDSKL+YDL DQGGEL SLRYDLTVPFARY+AMN IT+ KRY IAKVYR
Sbjct: 103 ELKETLTGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYR 162
Query: 550 RDNP--SKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLAVLNNYDE 609
RDNP ++GR+REFYQCDFDIAGQY+ M PD E +KI+ E+L EL++G
Sbjct: 163 RDNPAMTRGRYREFYQCDFDIAGQYDAMIPDAECLKIVHEILSELDLGD----------- 222
Query: 610 SASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDK 669
RIK+N RR+LDGM +CGVP + FRTICS++DKLDK
Sbjct: 223 -----------------------FRIKVNDRRILDGMFAVCGVPDNMFRTICSTVDKLDK 282
Query: 670 QSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNE 729
++ +K EMV EKGL+ E A++IG +V +G ++L +L Q+Q + Q+ + L +
Sbjct: 283 LPWEAVKNEMVNEKGLSEEAADQIGVYVGMQG-GMDLAERLLQDQK-MCQSTQACAGLTD 342
Query: 730 LEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVF-KGGTQRIFLDNSTDSDWEDF 789
+++LFS L+ DKVVFDLSLARGLDYYTG+IYEA+ + G + + S ++ E+
Sbjct: 343 IKLLFSYLQLFQVTDKVVFDLSLARGLDYYTGIIYEAILTQAGVAPVAPETSNEAPTEEC 402
Query: 790 VVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLIGMFGSK 849
V +GS+A GGRYD L+GMF K
Sbjct: 403 VT--------------------------------------VGSVAGGGRYDGLVGMFDPK 462
Query: 850 --QVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVLVSIL 909
+VP VGVS+GIER+F IMEQ + + R T+ +V+V+
Sbjct: 463 GRKVPCVGVSIGIERIFSIMEQKAE-----------------ASTEKIRTTEVQVMVAAA 516
Query: 910 GDD-LTQAAELASEIWSAKVHAEFLVNK--RVMKHIDRAKEARIPWIVFLGEREASEGIV 969
+ L + L +E+W+A + AE + K +++ + +E+ IP + LGE+E G+V
Sbjct: 523 QKNLLEERLRLITELWNAGIKAELMYKKSPKLLSQLQHCEESGIPLVAILGEQELKNGVV 516
Query: 970 KLKNVETFEEVTVSRSNIIDELKKR 987
KL+NV T +EV +SR+++I E+KKR
Sbjct: 583 KLRNVATRDEVDISRADLIAEIKKR 516
BLAST of CmoCh15G000190 vs. ExPASy TrEMBL
Match:
A0A6J1FE95 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1)
HSP 1 Score: 1617.8 bits (4188), Expect = 0.0e+00
Identity = 879/989 (88.88%), Postives = 879/989 (88.88%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKASGFDNLLRKIKEIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASGFDNLLRKIKEIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ---- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
IKLNHRRLLDGMLEICGVPSDKFRTICS
Sbjct: 601 ------------------------------YEIKLNHRRLLDGMLEICGVPSDKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ
Sbjct: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
IGSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------IGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRATKTEVL
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRATKTEVL 881
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 881
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVTVSRSNIIDELKKRLTP
Sbjct: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of CmoCh15G000190 vs. ExPASy TrEMBL
Match:
A0A6J1JV84 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1)
HSP 1 Score: 1592.8 bits (4123), Expect = 0.0e+00
Identity = 863/989 (87.26%), Postives = 873/989 (88.27%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA PAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPD+LTR+EARASLVVLLNKLIIS SSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVFEQSCY+L+GICALLDHQSAALSSIADAVASISCEASRADVSAFSL+DSGDGFASK
Sbjct: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAIS IPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLKREY+LVSELGLDEKY+EFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVK +LSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKASGFDNLL+KIKEIVESN SRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASGFDNLLKKIKEIVESNASRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ---- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
IKLNHRRLLDGMLEICGVPSDKFRTICS
Sbjct: 601 ------------------------------YEIKLNHRRLLDGMLEICGVPSDKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ
Sbjct: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
IGSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------IGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRATKTEVL
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRATKTEVL 881
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 881
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVTVSRSNIIDELKKRL P
Sbjct: 961 VKLKNVETFEEVTVSRSNIIDELKKRLAP 881
BLAST of CmoCh15G000190 vs. ExPASy TrEMBL
Match:
A0A6J1DGB6 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1)
HSP 1 Score: 1464.9 bits (3791), Expect = 0.0e+00
Identity = 796/994 (80.08%), Postives = 842/994 (84.71%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADP +E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH
Sbjct: 1 MADP-SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+F+P+FLTR+EARASL VLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVF+QSCY+LNGICALLDHQSAALSSIADAVA+ISCEASRADVSAF L+DSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIE GA S IPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 -GGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLK+EY+LVSEL LD++Y EFVHTVNVL TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKG-----GGLGSLENLVKDL 420
DVDIN NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKG GGLGSLEN VKDL
Sbjct: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
Query: 421 LSFLDPKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGV 480
LSFLDPKAS FDNLL+KIKEIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGV
Sbjct: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGV 480
Query: 481 FERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNG 540
FERHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNG
Sbjct: 481 FERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNG 540
Query: 541 ITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIG 600
ITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG
Sbjct: 541 ITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIG 600
Query: 601 QKGLAVLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKF 660
Q IKLNHR+LLDGMLEICGVP +KF
Sbjct: 601 Q----------------------------------YEIKLNHRKLLDGMLEICGVPPEKF 660
Query: 661 RTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPL 720
RTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+IGDFVKERGHPLELLSKLKQEQ PL
Sbjct: 661 RTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPL 720
Query: 721 LQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFL 780
LQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ
Sbjct: 721 LQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ---- 780
Query: 781 DNSTDSDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGR 840
+GSIAAGGR
Sbjct: 781 ---------------------------------------------------VGSIAAGGR 840
Query: 841 YDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRAT 900
YDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRAT
Sbjct: 841 YDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRAT 884
Query: 901 KTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGERE 960
KTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+RIPWIVFLGERE
Sbjct: 901 KTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRIPWIVFLGERE 884
Query: 961 ASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 990
SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 961 VSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
BLAST of CmoCh15G000190 vs. ExPASy TrEMBL
Match:
A0A6J1GRC1 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1)
HSP 1 Score: 1455.7 bits (3767), Expect = 0.0e+00
Identity = 786/989 (79.47%), Postives = 838/989 (84.73%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADP AE+SVITLGGKGSSL+S+S++AIAHGF+LVRIDSS+LERLASSSNN NAAS+KH
Sbjct: 1 MADP-AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQ 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+ +P++LTR+EARASLVVLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VVIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVF+QSCY+LNG+CALLDHQSAALSSIADAVA ISCEASRADVS F+L+DSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIESGAIS IPK HGCLREQAKLVHSKMRVELNS+VKIGK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED VRT LLSFAA+LWDLGKCSLDR KLI SN D+NMKASLAGLLDRECP
Sbjct: 241 -GGSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLK+ YRLVS+L LD+ Y EFVHTVNVL TVWKIV WEAIA F+TIEGG+LIGKGQ
Sbjct: 301 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVD+N ANEKVV K+EKKKKAVLGKG+SVVVQLIKD+LQGKGG LG LEN VKD+LSFLD
Sbjct: 361 DVDMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG-LENSVKDILSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKAS FDNLL KI+EIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASEFDNLLMKIREIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKL+YDL+DQGGELCSLRYDLTVPFARYVAMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ---- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
IKLNHR+LLDGMLEICGVP +KFRTICS
Sbjct: 601 ------------------------------YEIKLNHRKLLDGMLEICGVPPEKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGL +EIAERIGDFVKERGHPLELL+KLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SS AL+ELEILFSALEK+ CVDKVVFDLSLARGLDYYTGVIYEA+FKGGTQ
Sbjct: 721 SSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
+GSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------VGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRATKTE+L
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRATKTEIL 878
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAK++AEFL+NKRVMKHIDRAKE+RIPWIVFLGERE SEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRIPWIVFLGEREVSEGI 878
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVT+ RSNIIDELKKRL P
Sbjct: 961 VKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of CmoCh15G000190 vs. ExPASy TrEMBL
Match:
A0A6J1JT52 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1)
HSP 1 Score: 1452.6 bits (3759), Expect = 0.0e+00
Identity = 784/989 (79.27%), Postives = 837/989 (84.63%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADP AE+SVITLGGKGSSL+S+S++AIAHGF+LVRIDSS+LERLASSSNN NAAS+KH
Sbjct: 1 MADP-AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQ 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+ +PD+LTR+EARASLVVLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VVIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVF+QSCY+LNG+CALLDHQSAALSSIADAVA ISCEASRADVS F+L+DSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIESGAIS IPK HGCLREQAKLVHSKMRVELNS+VKIGK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGS+SSGTED VRT LLSFAAMLWDLGKCSLDR KLIL SN D+NMKASLAGLLDRECP
Sbjct: 241 -GGSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLK+ YRLVS+L LD+ Y EFVHTVNVL TVWKIV WEAIA F+TIEGG+LIGKGQ
Sbjct: 301 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVD+N ANEKV+ K+EKKKKAVLGKG+SVVVQLIKD+LQG+ G LG LEN VKDLLSFLD
Sbjct: 361 DVDMNRANEKVLNKSEKKKKAVLGKGTSVVVQLIKDRLQGESGDLG-LENSVKDLLSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKAS FDNLL KI+EIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASEFDNLLMKIREIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKL+YDL+DQGGELCSLRYDLTVPFARY+AMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ---- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
IKLNHR+LLDGMLEICGVP +KFRTICS
Sbjct: 601 ------------------------------YEIKLNHRKLLDGMLEICGVPPEKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGL +EIAERIGDFVKERGHPLELL+KLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SS AL+ELEILFSALEK+ CVDKVVFDLSLARGLDYYTGVIYEA+FKGGTQ
Sbjct: 721 SSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
+GSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------VGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQ LKDQN QTTRATKTE+L
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQHLKDQN-----------------QTTRATKTEIL 878
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAK++AEFL+NKRVMKHIDRAKE+RIPWIVFLGERE SEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRIPWIVFLGEREVSEGI 878
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVT+ RSNIIDELKKRL P
Sbjct: 961 VKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of CmoCh15G000190 vs. NCBI nr
Match:
XP_022938504.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >KAG6578315.1 Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1617.8 bits (4188), Expect = 0.0e+00
Identity = 879/989 (88.88%), Postives = 879/989 (88.88%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKASGFDNLLRKIKEIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASGFDNLLRKIKEIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ---- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
IKLNHRRLLDGMLEICGVPSDKFRTICS
Sbjct: 601 ------------------------------YEIKLNHRRLLDGMLEICGVPSDKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ
Sbjct: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
IGSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------IGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRATKTEVL
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRATKTEVL 881
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 881
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVTVSRSNIIDELKKRLTP
Sbjct: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of CmoCh15G000190 vs. NCBI nr
Match:
XP_023550553.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1601.3 bits (4145), Expect = 0.0e+00
Identity = 869/989 (87.87%), Postives = 878/989 (88.78%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADPAAELSVITLGGKGSSLASTSVYAIAHGF+LVRIDSSSLER++SSSNNPNAASIKHH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFALVRIDSSSLERISSSSNNPNAASIKHH 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPDFLTR+EARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDFLTREEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSL+DSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAIS IPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLI GSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLIWGSNGDENMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLKREYRLVSELGLDEKYVEFVH VNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SNESLKREYRLVSELGLDEKYVEFVHAVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKASGFDNLL+KIKEIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASGFDNLLKKIKEIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIG
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIG----- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
+Y+ IKLNHRRLLDGMLEICGVPSDKFRTICS
Sbjct: 601 ------------------HYE-----------IKLNHRRLLDGMLEICGVPSDKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ
Sbjct: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
IGSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------IGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRATKTEVL
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRATKTEVL 881
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 881
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVTVSRSNIIDELKKRLTP
Sbjct: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of CmoCh15G000190 vs. NCBI nr
Match:
XP_022993046.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])
HSP 1 Score: 1592.8 bits (4123), Expect = 0.0e+00
Identity = 863/989 (87.26%), Postives = 873/989 (88.27%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA PAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPD+LTR+EARASLVVLLNKLIIS SSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVFEQSCY+L+GICALLDHQSAALSSIADAVASISCEASRADVSAFSL+DSGDGFASK
Sbjct: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAIS IPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLKREY+LVSELGLDEKY+EFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVK +LSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKASGFDNLL+KIKEIVESN SRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASGFDNLLKKIKEIVESNASRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ---- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
IKLNHRRLLDGMLEICGVPSDKFRTICS
Sbjct: 601 ------------------------------YEIKLNHRRLLDGMLEICGVPSDKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ
Sbjct: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
IGSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------IGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRATKTEVL
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRATKTEVL 881
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 881
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVTVSRSNIIDELKKRL P
Sbjct: 961 VKLKNVETFEEVTVSRSNIIDELKKRLAP 881
BLAST of CmoCh15G000190 vs. NCBI nr
Match:
XP_022152462.1 (histidine--tRNA ligase, cytoplasmic [Momordica charantia])
HSP 1 Score: 1464.9 bits (3791), Expect = 0.0e+00
Identity = 796/994 (80.08%), Postives = 842/994 (84.71%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADP +E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH
Sbjct: 1 MADP-SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+F+P+FLTR+EARASL VLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVF+QSCY+LNGICALLDHQSAALSSIADAVA+ISCEASRADVSAF L+DSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIE GA S IPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 -GGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLK+EY+LVSEL LD++Y EFVHTVNVL TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKG-----GGLGSLENLVKDL 420
DVDIN NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKG GGLGSLEN VKDL
Sbjct: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
Query: 421 LSFLDPKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGV 480
LSFLDPKAS FDNLL+KIKEIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGV
Sbjct: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGV 480
Query: 481 FERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNG 540
FERHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNG
Sbjct: 481 FERHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNG 540
Query: 541 ITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIG 600
ITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IG
Sbjct: 541 ITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIG 600
Query: 601 QKGLAVLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKF 660
Q IKLNHR+LLDGMLEICGVP +KF
Sbjct: 601 Q----------------------------------YEIKLNHRKLLDGMLEICGVPPEKF 660
Query: 661 RTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPL 720
RTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+IGDFVKERGHPLELLSKLKQEQ PL
Sbjct: 661 RTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGDFVKERGHPLELLSKLKQEQCPL 720
Query: 721 LQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFL 780
LQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ
Sbjct: 721 LQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ---- 780
Query: 781 DNSTDSDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGR 840
+GSIAAGGR
Sbjct: 781 ---------------------------------------------------VGSIAAGGR 840
Query: 841 YDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRAT 900
YDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRAT
Sbjct: 841 YDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRAT 884
Query: 901 KTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGERE 960
KTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+RIPWIVFLGERE
Sbjct: 901 KTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRIPWIVFLGERE 884
Query: 961 ASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 990
SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 961 VSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
BLAST of CmoCh15G000190 vs. NCBI nr
Match:
KAG6602728.1 (Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 790/989 (79.88%), Postives = 839/989 (84.83%), Query Frame = 0
Query: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MADP AE+SVITLGGKGSSL+S+S++AIAHGF+LVRIDSS+LERLASSSNN NAAS+KH
Sbjct: 1 MADP-AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQ 60
Query: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+ +PD+LTR+EARASLVVLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VVIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
ELSVF+QSCY+LNG+CALLDHQSAALSSIADAVA ISCEASRADVS F+L+DSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIESGAIS IPK HGCLREQAKLVHSKMRVELNS+VKIGK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED VRT LLSFAAMLWDLGKCSLDR KLIL SN D+NMKASLAGLLDRECP
Sbjct: 241 -GGSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECP 300
Query: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLK+ YRLVS+L LD+ Y EFVHTVNVL TVWKIV WEAIA F+TIEGG+LIGKGQ
Sbjct: 301 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
DVD+N ANEKVV K+EKKKKAVLGKG+SVVVQLIKD+LQGKGG LG LEN VKDLLSFLD
Sbjct: 361 DVDMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG-LENSVKDLLSFLD 420
Query: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
PKAS FDNLL KI+EIVESNESRRLPKLP KGTRDFAKEQMAIRKKAFSIIEGVFERHG
Sbjct: 421 PKASEFDNLLMKIREIVESNESRRLPKLP--KGTRDFAKEQMAIRKKAFSIIEGVFERHG 480
Query: 481 ATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFK 540
ATALDTPAFELRETLMGKYGEDSKL+YDL+DQGGELCSLRYDLTVPFARYVAMNGITSFK
Sbjct: 481 ATALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFK 540
Query: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQKGLA 600
RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ
Sbjct: 541 RYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQ---- 600
Query: 601 VLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVPSDKFRTICS 660
IKLNHR+LLDGMLEICGVP +KFRTICS
Sbjct: 601 ------------------------------YEIKLNHRKLLDGMLEICGVPPEKFRTICS 660
Query: 661 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 720
SIDKLDKQSFDQIKREMVEEKGL +EIAERIGDFVKERGHPLELL+KLKQEQSPLLQNKG
Sbjct: 661 SIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVKERGHPLELLAKLKQEQSPLLQNKG 720
Query: 721 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTD 780
SS AL+ELEILFSALEK+ CVDKVVFDLSLARGLDYYTGVIYEA+FKGGTQ
Sbjct: 721 SSGALSELEILFSALEKAKCVDKVVFDLSLARGLDYYTGVIYEAIFKGGTQ--------- 780
Query: 781 SDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLI 840
+GSIAAGGRYDNLI
Sbjct: 781 ----------------------------------------------VGSIAAGGRYDNLI 840
Query: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVL 900
GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN QTTRATKTE+L
Sbjct: 841 GMFGSKQVPAVGVSLGIERVFVIMEQLLKDQN-----------------QTTRATKTEIL 878
Query: 901 VSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVFLGEREASEGI 960
VSILGDDLTQAAELASEIWSAK++AEFL+NKRVMKHIDRAKE+RIPWIVFLGERE SEGI
Sbjct: 901 VSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRIPWIVFLGEREVSEGI 878
Query: 961 VKLKNVETFEEVTVSRSNIIDELKKRLTP 990
VKLKNVETFEEVT+ RSNIIDELKKRL P
Sbjct: 961 VKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of CmoCh15G000190 vs. TAIR 10
Match:
AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 916.8 bits (2368), Expect = 1.5e-266
Identity = 538/1001 (53.75%), Postives = 666/1001 (66.53%), Query Frame = 0
Query: 5 AAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHHLF-V 64
AAE S ITLGGKGSSL+S+SVY +A G + VRIDSS++ER ++ N SIK F +
Sbjct: 2 AAERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGI 61
Query: 65 PDFLTRDEARASLVVLLNKLIIST-----SSGIRSVIPILIAETLNSNPETLQFEPLDVT 124
P LT +E RASL VLLNKLI+ST SS RSV+P+ I E LN E+L+ +DVT
Sbjct: 62 PQGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVT 121
Query: 125 PEELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFA 184
E V E+SC L GIC+++DH+S LS I D+VA++SCE ++AD+++FSLLDSGDG
Sbjct: 122 EGENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNG 181
Query: 185 SKEEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKI 244
K+ +G+A D+KV LNG K GK+E IS IP HG R K VH+ R ELNS VK
Sbjct: 182 DKDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKG 241
Query: 245 GKGGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRE 304
GK GS ++G +A+ T LL + +LG CS R KL DEN+K L+ ++
Sbjct: 242 GK-TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLS---EKI 301
Query: 305 CPSSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGEL-IG 364
C +E+LK Y+L L+E Y F H +N VW+IV EA+AAF + GGEL +
Sbjct: 302 CVENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQ 361
Query: 365 KGQDVD-INGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKL-----QGKGGGLGSLENL 424
K D D +K KKNE KKAV+GKG+S+V+Q IKD+L G + SL
Sbjct: 362 KSGDADKEESKTDKKKKKNE--KKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQC 421
Query: 425 VKDLLSFLDPKASGFDNLLRKIKEIVESNESRRLPKLPKSKGTRDFAKEQMAIRKKAFSI 484
+ +L+ +P+ FD+LL K+KEIVESNE+RRLPKLP KGTRDFAKEQMA+R+KAFSI
Sbjct: 422 GEQILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLP--KGTRDFAKEQMAVREKAFSI 481
Query: 485 IEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYV 544
I+ VF+RHGATALDTP FELRETLMGKYGEDSKLVYD+ADQGGELCSLRYDLTVPFARYV
Sbjct: 482 IQNVFKRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYV 541
Query: 545 AMNGITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDE 604
AMNGITSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDE
Sbjct: 542 AMNGITSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDE 601
Query: 605 LNIGQKGLAVLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNHRRLLDGMLEICGVP 664
L IG +Y+ +KLNHR+LLDGMLEICGVP
Sbjct: 602 LEIG--------DYE--------------------------VKLNHRKLLDGMLEICGVP 661
Query: 665 SDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQE 724
+KFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EIA+RIG+FVKE+G PLELLSKL+QE
Sbjct: 662 PEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIGNFVKEKGAPLELLSKLRQE 721
Query: 725 QSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQ 784
S L N+ S +AL+EL I+F AL++S C +++VFDLSLARGLDYYTGVI+EAV G
Sbjct: 722 GSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSLARGLDYYTGVIFEAVCIGAE- 781
Query: 785 RIFLDNSTDSDWEDFVVLEQSKEFKILLEIIFALEDHFSLMILPVTMCEDTIMCYIGSIA 844
+GSI
Sbjct: 782 -------------------------------------------------------VGSIG 841
Query: 845 AGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQLSKYNMDSFNSIVGKLQT 904
AGGRYDNLIGMFG+KQVPAVG+SLGIERVF IME+L + Q Q+
Sbjct: 842 AGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIMEELNEKQKQV----------------- 883
Query: 905 TRATKTEVLVSILGDD-LTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWIVF 964
R T+T+VLVSI+ D+ L +AAEL S++W AK++AE+LV+KR KH +RAKE+ IPW+V
Sbjct: 902 IRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKESGIPWMVM 883
Query: 965 LGEREASEGIVKLKNV----ETFEEVTVSRSNIIDELKKRL 988
+GE+E S V LK + E E+ T +R ++ELKK L
Sbjct: 962 VGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of CmoCh15G000190 vs. TAIR 10
Match:
AT3G46100.1 (Histidyl-tRNA synthetase 1 )
HSP 1 Score: 122.1 bits (305), Expect = 2.5e-27
Identity = 125/535 (23.36%), Postives = 217/535 (40.56%), Query Frame = 0
Query: 452 KGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LVYDLA 511
KGTRDF E M +R F+ + V +G +D P E + K GE+ + +Y
Sbjct: 73 KGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCFE 132
Query: 512 DQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDFD 571
D+G +LR +LT AR V G + K + I + +R + ++GR RE YQ + D
Sbjct: 133 DRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNMD 192
Query: 572 IAGQYEKMGPDFEVIKILTELLDELNIGQKGLAVLNNYDESASIAQNINLIYYKHIHPYY 631
I G ++ + E+I + + I +
Sbjct: 193 IIG-VPQVTAEAELISSIVTFFKRIGITASDVG--------------------------- 252
Query: 632 HMPRRIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEI 691
K++ R++L +L+ GVP D F +C IDK++K D+IK+E+ G++ +
Sbjct: 253 -----FKVSSRKVLQELLKKYGVPEDLFGRVCIIIDKIEKIPIDEIKKEL-GFTGISEDA 312
Query: 692 AERIGDFVKERGHPLELLS-KLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVF 751
E++ L++LS K + ++ G+ +A+ +L+ LFS EK + + F
Sbjct: 313 IEQL----------LQVLSVKSLDDLEDII--GGAGEAIADLKQLFSLAEKFGYSEWIQF 372
Query: 752 DLSLARGLDYYTGVIYEAVFKGGTQRIFLDNSTDSDWEDFVVLEQSKEFKILLEIIFALE 811
D S+ RGL YYTG+++E + G R
Sbjct: 373 DASVVRGLAYYTGIVFEGFDRKGKLR---------------------------------- 432
Query: 812 DHFSLMILPVTMCEDTIMCYIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 871
+I GGRYD L+ +G PA G G ++ +
Sbjct: 433 ----------------------AICGGGRYDRLLSTYGGDDFPACGFGFG----DAVIVE 485
Query: 872 LLKDQNQLSKYNMDSFNSIVGKLQTTRATKTEVLVSILGDDLT-QAAELASEIWSAKVHA 931
LLK+++ L + + E +V L DL AA +A+ +
Sbjct: 493 LLKEKDLLPELGQE----------------VENIVCALDKDLQGAAATVATALRDKGQTV 485
Query: 932 EFLVNKRVMKHI-DRAKEARIPWIVFLGEREASEGIVKLKNVETFEEVTVSRSNI 980
+ ++ + +K + RA +V +G+ E +G V +K + + E+ V S++
Sbjct: 553 DLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLSSGEQFQVKLSDL 485
BLAST of CmoCh15G000190 vs. TAIR 10
Match:
AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 72.0 bits (175), Expect = 3.0e-12
Identity = 57/218 (26.15%), Postives = 90/218 (41.28%), Query Frame = 0
Query: 452 KGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LVYDLA 511
KGTRDF E M +R F+ + V G +D P E + K GE+ + +Y
Sbjct: 73 KGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQLYCFE 132
Query: 512 DQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDFD 571
D+G +LR +LT AR V G + K + + + +R + ++GR RE YQ + D
Sbjct: 133 DRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHYQWNMD 192
Query: 572 IAGQYEKMGPDFEVIKILTELLDELNIGQKGLAVLNNYDESASIAQNINLIYYKHIHPYY 631
I G E + + E+I + + I +
Sbjct: 193 IIGVPE-VTAEAELISSIMTFFKRIGITASDVG--------------------------- 252
Query: 632 HMPRRIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKL 666
K++ R++L L+ GVP D F +C IDK+
Sbjct: 253 -----FKVSSRKVLQEFLKKYGVPEDLFGRVCFIIDKV 257
BLAST of CmoCh15G000190 vs. TAIR 10
Match:
AT3G59410.1 (protein kinase family protein )
HSP 1 Score: 63.5 bits (153), Expect = 1.1e-09
Identity = 83/376 (22.07%), Postives = 159/376 (42.29%), Query Frame = 0
Query: 408 LENLVKDLLSFLDPKASGFDNLLRKI--KEIVE--SNESRRLPKLPKSKGTRDFAKEQMA 467
L+N+++ + + D +S +D ++ I +E++E S++S R +L + +
Sbjct: 739 LDNILRIMQTSED--SSVYDRVVSVIFDEEVLEMKSHQSSR-SRLCADDSYIQYTEINTE 798
Query: 468 IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDL 527
+R I + VF +H A L+ + L+ + S+ L GG++ L Y+L
Sbjct: 799 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 858
Query: 528 TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 587
+PF ++++N +SFKRY+I+ VYRR NP Q DFDI G +
Sbjct: 859 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 918
Query: 588 DFEVIKILTELLDELNIGQKGLAVLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNH 647
+ EV+K++ ++ H ++ I LNH
Sbjct: 919 EAEVLKVIVDIT---------------------------------THIFHRGSCDIHLNH 978
Query: 648 RRLLDGMLEICGVPSD---KFRTICSSIDKLDKQSFDQ------IKREMVEEKGLTVEIA 707
LLD + G+ ++ K + S + L QS ++ I+R++++E L +
Sbjct: 979 GDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVV 1038
Query: 708 ERIGDFVKE-RGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFD 764
R+ G + L +L+ L ++ + AL+EL L + L + V D
Sbjct: 1039 NRLQTVASRFCGDADQALPRLR---GALRADRPTRKALDELSNLLTYLRVWRIEEHVHID 1063
BLAST of CmoCh15G000190 vs. TAIR 10
Match:
AT3G59410.2 (protein kinase family protein )
HSP 1 Score: 63.5 bits (153), Expect = 1.1e-09
Identity = 83/376 (22.07%), Postives = 159/376 (42.29%), Query Frame = 0
Query: 408 LENLVKDLLSFLDPKASGFDNLLRKI--KEIVE--SNESRRLPKLPKSKGTRDFAKEQMA 467
L+N+++ + + D +S +D ++ I +E++E S++S R +L + +
Sbjct: 763 LDNILRIMQTSED--SSVYDRVVSVIFDEEVLEMKSHQSSR-SRLCADDSYIQYTEINTE 822
Query: 468 IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDL 527
+R I + VF +H A L+ + L+ + S+ L GG++ L Y+L
Sbjct: 823 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 882
Query: 528 TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 587
+PF ++++N +SFKRY+I+ VYRR NP Q DFDI G +
Sbjct: 883 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 942
Query: 588 DFEVIKILTELLDELNIGQKGLAVLNNYDESASIAQNINLIYYKHIHPYYHMPRRIKLNH 647
+ EV+K++ ++ H ++ I LNH
Sbjct: 943 EAEVLKVIVDIT---------------------------------THIFHRGSCDIHLNH 1002
Query: 648 RRLLDGMLEICGVPSD---KFRTICSSIDKLDKQSFDQ------IKREMVEEKGLTVEIA 707
LLD + G+ ++ K + S + L QS ++ I+R++++E L +
Sbjct: 1003 GDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRPQSSERKLKWVFIRRQLLQELKLPEAVV 1062
Query: 708 ERIGDFVKE-RGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFD 764
R+ G + L +L+ L ++ + AL+EL L + L + V D
Sbjct: 1063 NRLQTVASRFCGDADQALPRLR---GALRADRPTRKALDELSNLLTYLRVWRIEEHVHID 1087
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4IYF8 | 2.1e-265 | 53.75 | Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760... | [more] |
| P93422 | 9.2e-200 | 47.93 | Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=... | [more] |
| E9QI36 | 7.2e-120 | 45.57 | Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2 | [more] |
| Q61035 | 5.7e-117 | 43.97 | Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2 | [more] |
| P70076 | 9.7e-117 | 43.19 | Histidine--tRNA ligase, cytoplasmic OS=Takifugu rubripes OX=31033 GN=hars1 PE=3 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1FE95 | 0.0e+00 | 88.88 | Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1 | [more] |
| A0A6J1JV84 | 0.0e+00 | 87.26 | Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1 | [more] |
| A0A6J1DGB6 | 0.0e+00 | 80.08 | Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1 | [more] |
| A0A6J1GRC1 | 0.0e+00 | 79.47 | Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1 | [more] |
| A0A6J1JT52 | 0.0e+00 | 79.27 | Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022938504.1 | 0.0e+00 | 88.88 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >KAG6578315.1 Hist... | [more] |
| XP_023550553.1 | 0.0e+00 | 87.87 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_022993046.1 | 0.0e+00 | 87.26 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] | [more] |
| XP_022152462.1 | 0.0e+00 | 80.08 | histidine--tRNA ligase, cytoplasmic [Momordica charantia] | [more] |
| KAG6602728.1 | 0.0e+00 | 79.88 | Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. soro... | [more] |
