CmoCh14G005770 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh14G005770
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	2-isopropylmalate synthase
Location	Cmo_Chr14: 2941862 .. 2945094 (-)
RNA-Seq Expression	CmoCh14G005770
Synteny	CmoCh14G005770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTACGAGACCTCAAACCCAATCCAGCTATGTCCATGCTAACTATGCCGGTTTCAATGTCGATGTCGATGTCGAAGCCGAATCATGACGTTTGTATTTCCCGTCATCAAATAACATCATGTCATGTAGTGTCTTCGGTTCGACAACGTCCACCCTACACCCCAAATTCCATCCTGGACGCTCCCTATGTACGCGTATTTGACACCACTCTCCGTGACGGCGAGCAATCCCCCGGCACAGCCCTCACGTTGAAGCAGAAGCTTCTCATTGCCCGTCAGCTTGTGAAGCTGAGGGTCGATGCAATTGAGGCGGGATTCCCGGCAGCGTCGAGGCAGGACTTTGAGGCTGTGGAGATGGTTGCGAAAGAGGTTGGTAATGAGGTAGGTGAGGACGGATACGTGCCGGTGATTTGCGGGTTCTCGAGGTGTACCGAGAGCGACGTTCGAGCTGCTTGGGAGGCAGTGAAGTATGCTAAGAGACCAAGAATACACATGTTCATTGCAACGAGTGAGATTCATTTGACGCATAAGCTTAGAAAGACGAGAGGGGAAGTGGTTGAGATTGCTAGGAATATGGTGAGGTTTGCACGAAGCTTGGGTTTTGATGATGTTGAGTTTAGCCCTGAGGATGCTGGCAGGTTTGATCTTTAATTATCTCTACTTGAGCTAGTTCGAGTTTGTTAGATCCCATATCGGTTGGAGAGGAGAACGAAACACTCTTTATAAGGGTGTGGAAACCTTTTCCTAGCAGACGTGTTTTGAATAAGATTGTTTTGCTGGCGTTTAAGGGAAAATCCTAGTGGTGGGCTTGGGCCGTTATAAATGGTATCAAAGCCATACACTAGGCAATGTGCCAGTGAGAAGGTTGTTCCTTGAAAAGGGTAGACACGAGACGGTGTGCTATTAAGAACCCTGGGCTCCGAAGGGAAATGAATTTGGTGGAAGTCCCACATAAATTGGAGAAGGAACGAGTGCCAGTGAGGATGCTGGGCCCTGAAGGGGGTGGATTGTTAGATCCCACATCGGGTTGTGGAGGAGAACGAAACATCCTTTATAAGGGTGTGGAAACCTTTCCTTAGCCTAGCAGACGCGTTTTAAACCTTTGAGGGGAAGCCCGAAAGGGAAGGCCTTTTCCTAGCCTAACCCTGAATGGGGGTGGATTGTTAGATCCCACAGCGGTTAGGGAGGAGAACGAAACACCCTTTATAACGGTGTAAAAGTCTTTCCTTAGTAAACGCGTTTTAAAGTCTTGAGAGCGAAAGTCAAAGGAAAAGTCTAAATAAGACAATATCTACTAGCGGTGGGGTTACACAATTTCGCAGTTCGTATATATTTGAATTGAGCTGGCTGGAGTTGTCATTTGTTGGGTGTGGTATCAGATCAGATAAAGAGTTTCTGTATCAAATTCTTGGTGAAGTGATAAGAGCTGGGGCGACAACTCTGAACATAACAGACACAGTGGGGTATACAGTGCCAGCTGAATATGGGAAGCTGATAGCTGATATAAAATGTAATACTCCTGGCACTCAAAATGTTATCATTTCAACGCATTGCCAGAATGACCTTGGGCTAGCAACTGCCAATACAATAGCAGTAAGTGATACTAACCCTCTATATGTTCTTGCTCCAAATTCCATTCCTCTCATATTTTTGTTTTGTAGGGGGCATGCGGTGGTGCAAGGCAAGTTGAGGTCACAATAAATGGCATAGGTGAGAAAGCGGGAAATGCTTCACTCGAGGAGGTAATCTGTTCTTTCATTTATCAACCGATCTATGCTCAAATTGGTCCTATAATTTTTGTAACACTCCATGCCTAAAATTTGGCACCTAATGGCTTCGGTATTCCCCTGCGGTATAGCACTTTACTTACTCTTTGCTTCTAAGAGTGAAGACTATCTCTACAGACCCACACAAGTCCTTCTAGCATGTTCTGTCCTTGTTTGCATGCGTCCTAGGAAATTAATGGTCCCGGTATCCTCCCATGTACTTACAGACCGACACAAACCTTTCTAGCATGTTTTGACCCAACATAGAATTGATCCAAGCAAAACACGTTTAACCATGAAGTTTTTATAGGACAGACAATGGATATATGTATCTTGTTGATATAGATAGTAACTTCCAATTCTTTTAAGCTTTTCTCGGTCATCTTATCCTTAAGATCACTCTCATTCAAATGCGGTCACGGTTCATTCATGTATTATTTCTTTATTCGAGTTAATGCACCATTCTACGATACATGATGTGTTTAGATGGTAATGACCCTACGATGTCGTGGAGAGCATGTGTTTGGAGGTCTTTACACTGGAATTAACTCGAGACATATCTTTTCAGCCAGTAAAATAGTAATTAGTTACCAGAAAGCTTCTAAAAAAGTTACCAAAAAGCTTAATTATTTGAGAAGGTTGGCCAAATTGAGTGTCATTGTTGGTCAGGTAGAAGAGTACACTGGGTTGAATGTGCAGCCCCACAAGGCCATTGTTGGAGCCAATGCCTTTGCTCATCAAAGTGGCATCCACCAGGTTGCCCTTCTTCCATTTTTACTCTTGTTGAGGCTTAAATGGTATCCTAAATATGATCATTGATACTCAGGATGGGATGCTTAAGCACAGAGGCACTTACGAAATCATATCACCAGAAGATATTGGCTATCAACGATCCAATCATGTTGTTATTGTTCTTGGAAAGCTCAGGTATGCCCTGTTTCTTTACTGCTATGAACCTCGATAAAGTTCTTATTTTTTTCAACCAAAGCTCCACCTTTGACTTTCTTATGTGTATAGTGGACGTCATGCTCTTAAAAGTTACCTTCGAAAGGTATATTGTGATAACGTTTTTCATAAGCTATCATATCATTTATTTCATGTAGGGGCTTCATGCAGCTGATATTGATTGAATAATGTTATACCTCATTGTCTTTTCAGCTTGGCTATGAGATTGACGAGGACAAGCTCGACAACGTATTTTGGCGTTTCAAAGCTTTAGCTGAACAGAAAAATGTAATTTAGGTGATACCCATTTGAGATTATTCTACCTAGGTTACCATTCATCAATACTCCTTACTTGTTTTTGAATTTTCAAAGAGTTATAGATGCGGATCTTATAGCACTGGTATCTGATGGAGTTTCTAAGAAAGTTGACATCAAAGTGACTGAAAACCGAACATAGTTTGTGTGAAATTTTAGATTTCATTATAGTTGAATCGTTTTTTAAGTAAATTTATCGTGGGATGTGC

mRNA sequence

CGTACGAGACCTCAAACCCAATCCAGCTATGTCCATGCTAACTATGCCGGTTTCAATGTCGATGTCGATGTCGAAGCCGAATCATGACGTTTGTATTTCCCGTCATCAAATAACATCATGTCATGTAGTGTCTTCGGTTCGACAACGTCCACCCTACACCCCAAATTCCATCCTGGACGCTCCCTATGTACGCGTATTTGACACCACTCTCCGTGACGGCGAGCAATCCCCCGGCACAGCCCTCACGTTGAAGCAGAAGCTTCTCATTGCCCGTCAGCTTGTGAAGCTGAGGGTCGATGCAATTGAGGCGGGATTCCCGGCAGCGTCGAGGCAGGACTTTGAGGCTGTGGAGATGGTTGCGAAAGAGGTTGGTAATGAGGTAGGTGAGGACGGATACGTGCCGGTGATTTGCGGGTTCTCGAGGTGTACCGAGAGCGACGTTCGAGCTGCTTGGGAGGCAGTGAAGTATGCTAAGAGACCAAGAATACACATGTTCATTGCAACGAGTGAGATTCATTTGACGCATAAGCTTAGAAAGACGAGAGGGGAAGTGGTTGAGATTGCTAGGAATATGGTGAGGTTTGCACGAAGCTTGGGTTTTGATGATGTTGAGTTTAGCCCTGAGGATGCTGGCAGATCAGATAAAGAGTTTCTGTATCAAATTCTTGGTGAAGTGATAAGAGCTGGGGCGACAACTCTGAACATAACAGACACAGTGGGGTATACAGTGCCAGCTGAATATGGGAAGCTGATAGCTGATATAAAATGTAATACTCCTGGCACTCAAAATGTTATCATTTCAACGCATTGCCAGAATGACCTTGGGCTAGCAACTGCCAATACAATAGCAGGGGCATGCGGTGGTGCAAGGCAAGTTGAGGTCACAATAAATGGCATAGGTGAGAAAGCGGGAAATGCTTCACTCGAGGAGGTAGAAGAGTACACTGGGTTGAATGTGCAGCCCCACAAGGCCATTGTTGGAGCCAATGCCTTTGCTCATCAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAGAGGCACTTACGAAATCATATCACCAGAAGATATTGGCTATCAACGATCCAATCATGTTGTTATTGTTCTTGGAAAGCTCAGTGGACGTCATGCTCTTAAAAGTTACCTTCGAAAGCTTGGCTATGAGATTGACGAGGACAAGCTCGACAACGTATTTTGGCGTTTCAAAGCTTTAGCTGAACAGAAAAATGTAATTTAGGTGATACCCATTTGAGATTATTCTACCTAGGTTACCATTCATCAATACTCCTTACTTGTTTTTGAATTTTCAAAGAGTTATAGATGCGGATCTTATAGCACTGGTATCTGATGGAGTTTCTAAGAAAGTTGACATCAAAGTGACTGAAAACCGAACATAGTTTGTGTGAAATTTTAGATTTCATTATAGTTGAATCGTTTTTTAAGTAAATTTATCGTGGGATGTGC

Coding sequence (CDS)

ATGTCCATGCTAACTATGCCGGTTTCAATGTCGATGTCGATGTCGAAGCCGAATCATGACGTTTGTATTTCCCGTCATCAAATAACATCATGTCATGTAGTGTCTTCGGTTCGACAACGTCCACCCTACACCCCAAATTCCATCCTGGACGCTCCCTATGTACGCGTATTTGACACCACTCTCCGTGACGGCGAGCAATCCCCCGGCACAGCCCTCACGTTGAAGCAGAAGCTTCTCATTGCCCGTCAGCTTGTGAAGCTGAGGGTCGATGCAATTGAGGCGGGATTCCCGGCAGCGTCGAGGCAGGACTTTGAGGCTGTGGAGATGGTTGCGAAAGAGGTTGGTAATGAGGTAGGTGAGGACGGATACGTGCCGGTGATTTGCGGGTTCTCGAGGTGTACCGAGAGCGACGTTCGAGCTGCTTGGGAGGCAGTGAAGTATGCTAAGAGACCAAGAATACACATGTTCATTGCAACGAGTGAGATTCATTTGACGCATAAGCTTAGAAAGACGAGAGGGGAAGTGGTTGAGATTGCTAGGAATATGGTGAGGTTTGCACGAAGCTTGGGTTTTGATGATGTTGAGTTTAGCCCTGAGGATGCTGGCAGATCAGATAAAGAGTTTCTGTATCAAATTCTTGGTGAAGTGATAAGAGCTGGGGCGACAACTCTGAACATAACAGACACAGTGGGGTATACAGTGCCAGCTGAATATGGGAAGCTGATAGCTGATATAAAATGTAATACTCCTGGCACTCAAAATGTTATCATTTCAACGCATTGCCAGAATGACCTTGGGCTAGCAACTGCCAATACAATAGCAGGGGCATGCGGTGGTGCAAGGCAAGTTGAGGTCACAATAAATGGCATAGGTGAGAAAGCGGGAAATGCTTCACTCGAGGAGGTAGAAGAGTACACTGGGTTGAATGTGCAGCCCCACAAGGCCATTGTTGGAGCCAATGCCTTTGCTCATCAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAGAGGCACTTACGAAATCATATCACCAGAAGATATTGGCTATCAACGATCCAATCATGTTGTTATTGTTCTTGGAAAGCTCAGTGGACGTCATGCTCTTAAAAGTTACCTTCGAAAGCTTGGCTATGAGATTGACGAGGACAAGCTCGACAACGTATTTTGGCGTTTCAAAGCTTTAGCTGAACAGAAAAATGTAATTTAG

Protein sequence

MSMLTMPVSMSMSMSKPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLEEVEEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQKNVI

Homology

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BLAST of CmoCh14G005770 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 1.8e-154
Identity = 275/393 (69.97%), Postives = 317/393 (80.66%), Query Frame = 0


Query: 38  RQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFP 97
           R+RP Y PN I D  YVR+FDTTLRDGEQSPG  LT K+KL IARQL KL VD IEAGFP
Sbjct: 71  RRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFP 130

Query: 98  AASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFI 157
           AAS+ DFEAV+ +A+ VGN V E+GYVPVICG SRC + D+  AWEAVKYAKRPRIH FI
Sbjct: 131 AASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFI 190

Query: 158 ATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVI 217
           ATS+IHL +KL+K++ EV+EIARNMVRFARSLG +DVEFSPEDAGRS++E+LY+ILGEVI
Sbjct: 191 ATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVI 250

Query: 218 RAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGAC 277
           +AGATTLNI DTVG T+P+E+G+LIADIK NTPG QNVIISTHCQNDLGL+TANT++GA 
Sbjct: 251 KAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAH 310

Query: 278 GGARQVEVTINGIGEKAGNASLEE-------------------------------VEEYT 337
            GARQVEVTINGIGE+AGNASLEE                               VEEYT
Sbjct: 311 SGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYT 370

Query: 338 GLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGR 397
           G+  QPHKAIVGANAFAH+SGIHQDGMLKH+GTYEI+SPE+IG +RSN   IVLGKLSGR
Sbjct: 371 GMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGR 430

Query: 398 HALKSYLRKLGYEIDEDKLDNVFWRFKALAEQK 400
           HALK  L +LGY +D+ +L N+FWRFKA+AEQK
Sbjct: 431 HALKDRLNELGYVLDDGQLSNLFWRFKAVAEQK 463

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BLAST of CmoCh14G005770 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 538.1 bits (1385), Expect = 8.4e-152
Identity = 278/435 (63.91%), Postives = 329/435 (75.63%), Query Frame = 0


Query: 7   PVSMSMSMSKPNHDVCISRHQIT---SCHVV--------SSVRQRPPYTPNSILDAPYVR 66
           P+S S    +P+H   IS    T   SC +         +  R RP Y PN I D  YVR
Sbjct: 32  PISSSFRF-QPSHHRSISLRSQTLRLSCSISDPSPLPPHTPRRPRPEYIPNRISDPNYVR 91

Query: 67  VFDTTLRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVG 126
           VFDTTLRDGEQSPG  LT K+KL IARQL KL VD IEAGFPAAS+ DFEAV+ +A+ VG
Sbjct: 92  VFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVG 151

Query: 127 NEVGEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEV 186
           N V E+GYVPVICG SRC + D+  AW+AVKYAKRPRIH FIATS+IHL +KL+KT+ EV
Sbjct: 152 NTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKKTKAEV 211

Query: 187 VEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVP 246
           +EIAR+MVRFARSLG +DVEFSPEDAGRS++E+LY+ILGEVI+AGATTLNI DTVG T+P
Sbjct: 212 IEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLP 271

Query: 247 AEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAG 306
           +E+G+LI D+K NTPG +NV+ISTHCQNDLGL+TANT++GA  GARQ+EVTINGIGE+AG
Sbjct: 272 SEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGARQMEVTINGIGERAG 331

Query: 307 NASLEE-------------------------------VEEYTGLNVQPHKAIVGANAFAH 366
           NASLEE                               VEEYTG+  QPHKAIVGANAFAH
Sbjct: 332 NASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAH 391

Query: 367 QSGIHQDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDK 400
           +SGIHQDGMLKH+GTYEII PE+IG +RSN   IVLGKLSGRHALK  L +LGY++D+++
Sbjct: 392 ESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQ 451

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BLAST of CmoCh14G005770 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 7.1e-151
Identity = 266/409 (65.04%), Postives = 321/409 (78.48%), Query Frame = 0


Query: 25  RHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIARQL 84
           +H  ++    S   +RP Y P+ I D  YVR+FDTTLRDGEQSPG  +T K+KL +ARQL
Sbjct: 19  KHYYSTFIRCSISNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQL 78

Query: 85  VKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCTESDVRAAWEA 144
            KL VD IEAGFPA+S  DFE+V+++A+E+GN   E+G+VPVICG SRC +SD+  AWEA
Sbjct: 79  AKLGVDIIEAGFPASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEA 138

Query: 145 VKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDAGRS 204
           VKYAK+PR+H FIATSEIH+ +KL+ +R +VVE AR+MV +ARSLG +DVEFSPEDAGRS
Sbjct: 139 VKYAKKPRVHTFIATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRS 198

Query: 205 DKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQND 264
           D+EFLY ILGEVI+AGATTLNI DTVGYTVP+E+G+LI DIK NTPG +NVIISTHCQND
Sbjct: 199 DREFLYDILGEVIKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQND 258

Query: 265 LGLATANTIAGACGGARQVEVTINGIGEKAGNASLEE----------------------- 324
           LGL+TANT+AGAC GARQ+EVTINGIGE+AGNASLEE                       
Sbjct: 259 LGLSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQ 318

Query: 325 --------VEEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGYQRS 384
                   VEEY+GL VQPHKAIVGANAFAH+SGIHQDGMLKH+ TYEIISP+D+G  RS
Sbjct: 319 HIVPSSKMVEEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEIISPDDVGLSRS 378

Query: 385 NHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQKNVI 403
           N   IVLGKLSGRHALKS + +LGY+ID  +L+++FWRFK++AE+K  I
Sbjct: 379 NEAGIVLGKLSGRHALKSKMLELGYDIDGKELEDLFWRFKSVAEKKKKI 427

[Close]

BLAST of CmoCh14G005770 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 9.4e-143
Identity = 263/419 (62.77%), Postives = 314/419 (74.94%), Query Frame = 0


Query: 18  NHDVCISRHQITSCH---VVSSVRQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTL 77
           N    IS+H  +S     V  S+R+ P YTP+ I D  YVR+FDTTLRDGEQSPG  +T 
Sbjct: 32  NFKPSISKHSNSSFSIPVVRCSIRRIPEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTT 91

Query: 78  KQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCT 137
           K+KL +ARQ  KL VD IEAGFPA+S  D EAV+++AKEVGN V E+ YVPVICG +RC 
Sbjct: 92  KEKLDVARQSAKLGVDIIEAGFPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCN 151

Query: 138 ESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDV 197
           + D+  AWEAVKYAK+PRIH FIATSE+H+ +KL+ +R +VVE AR+MV +ARS+G +DV
Sbjct: 152 KKDIDKAWEAVKYAKKPRIHTFIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDV 211

Query: 198 EFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQN 257
           EFSPEDAGRSD EFLY ILGEVI+AGATTLNI DTVGYTVP E+G+LIA IK NTPG ++
Sbjct: 212 EFSPEDAGRSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVED 271

Query: 258 VIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLEE------------- 317
           VIISTHCQNDLGL+TANT+AGAC GARQ+EVTINGIGE+AGNASLEE             
Sbjct: 272 VIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVL 331

Query: 318 ------------------VEEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEII 377
                             VE  +GL+VQPHKAIVGANAF H+SGIHQDGMLKH+ TYEII
Sbjct: 332 GGLYTGINTQHILMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKDTYEII 391

Query: 378 SPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQKNVI 403
           SPEDIG  R+N   IV GKLSG    K  + +LGYEI+  +LD++FWRFK++AE+K  I
Sbjct: 392 SPEDIGLNRANESGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEKKKKI 450

[Close]

BLAST of CmoCh14G005770 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 4.8e-139
Identity = 249/394 (63.20%), Postives = 300/394 (76.14%), Query Frame = 0


Query: 40  RPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAA 99
           RP Y PN I D+ YVR+ DTTLRDGEQSPG  +T K+KL IARQLVKL VD I+ GFP+A
Sbjct: 19  RPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVKLGVDIIQPGFPSA 78

Query: 100 SRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIAT 159
           S  DF AV+M+A+EVGN V +DGYVPVI GF RC E D+  AWEAVKYAKRPR+   IAT
Sbjct: 79  SNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVKYAKRPRLCTSIAT 138

Query: 160 SEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRA 219
           S IH+ HKLRK++ +V++IAR+MV+FARSLG +D++F  EDA RSD+EFLY+ILG VI A
Sbjct: 139 SPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDREFLYEILGVVIEA 198

Query: 220 GATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGG 279
           GATT+NI DTVG  +P E GKLI DIK NTPG  NVIISTHC NDLGLATANTI GA  G
Sbjct: 199 GATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLGLATANTIEGARTG 258

Query: 280 ARQVEVTINGIGEKAGNASLEE-------------------------------VEEYTGL 339
           ARQ+EVTINGIGE+AGNASLEE                               VEEY+G+
Sbjct: 259 ARQLEVTINGIGERAGNASLEEVVMALASKGDHALNGLYTRINTRHILETSKMVEEYSGM 318

Query: 340 NVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHA 399
           ++QPHK +VGANAF H SGIHQDGMLKH+GTYE ISPE+IG++R+  + IVLGKLSG  A
Sbjct: 319 HLQPHKPLVGANAFVHASGIHQDGMLKHKGTYETISPEEIGHKRTTRIGIVLGKLSGSQA 378

Query: 400 LKSYLRKLGYEIDEDKLDNVFWRFKALAEQKNVI 403
           L+  L +LGY++ ED++D+VFW+FKA+AE+K V+
Sbjct: 379 LRKRLEELGYDLKEDEVDSVFWQFKAMAEKKKVV 412

[Close]

BLAST of CmoCh14G005770 vs. ExPASy TrEMBL
Match: A0A6J1F8D9 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441807 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.6e-223
Identity = 402/433 (92.84%), Postives = 402/433 (92.84%), Query Frame = 0


Query: 1   MSMLTMPVSMSMSMSKPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 60
           MSMLTMPVSMSMSMSKPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT
Sbjct: 1   MSMLTMPVSMSMSMSKPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 60

Query: 61  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 120
           LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE
Sbjct: 61  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 120

Query: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 180
           DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR
Sbjct: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 180

Query: 181 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 240
           NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK
Sbjct: 181 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 240

Query: 241 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 300
           LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE
Sbjct: 241 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 300

Query: 301 E-------------------------------VEEYTGLNVQPHKAIVGANAFAHQSGIH 360
           E                               VEEYTGLNVQPHKAIVGANAFAHQSGIH
Sbjct: 301 EMVMTLRCRGEHVFGGLYTGINSRHIFSASKIVEEYTGLNVQPHKAIVGANAFAHQSGIH 360

Query: 361 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 403
           QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF
Sbjct: 361 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 420

[Close]

BLAST of CmoCh14G005770 vs. ExPASy TrEMBL
Match: A0A6J1F3I8 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441807 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 6.2e-222
Identity = 400/431 (92.81%), Postives = 400/431 (92.81%), Query Frame = 0


Query: 1   MSMLTMPVSMSMSMSKPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 60
           MSMLTMPVSMSMSMSKPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT
Sbjct: 1   MSMLTMPVSMSMSMSKPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 60

Query: 61  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 120
           LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE
Sbjct: 61  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 120

Query: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 180
           DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR
Sbjct: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 180

Query: 181 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 240
           NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK
Sbjct: 181 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 240

Query: 241 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 300
           LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE
Sbjct: 241 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 300

Query: 301 E-------------------------------VEEYTGLNVQPHKAIVGANAFAHQSGIH 360
           E                               VEEYTGLNVQPHKAIVGANAFAHQSGIH
Sbjct: 301 EMVMTLRCRGEHVFGGLYTGINSRHIFSASKIVEEYTGLNVQPHKAIVGANAFAHQSGIH 360

Query: 361 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 401
           QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF
Sbjct: 361 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 420

[Close]

BLAST of CmoCh14G005770 vs. ExPASy TrEMBL
Match: A0A6J1J7V5 (2-isopropylmalate synthase OS=Cucurbita maxima OX=3661 GN=LOC111482524 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.1e-167
Identity = 303/409 (74.08%), Postives = 335/409 (81.91%), Query Frame = 0


Query: 22  CISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIA 81
           C  +  I S     S  +RPPY PN I D+ YVR+FDTTLRDGEQSPG +LT K+K+ IA
Sbjct: 57  CCQKESIVSNSESKSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKVDIA 116

Query: 82  RQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCTESDVRAA 141
           RQL KL VD IEAGFPAAS++DFEAV+M+AKEVGN V EDGYVPVICG SRC E D+R A
Sbjct: 117 RQLTKLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTA 176

Query: 142 WEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDA 201
           WEAVKY+KRPRIH FIATSEIH+ HKLRKTR +V+EIARNMV+FARSLG DDVEFSPEDA
Sbjct: 177 WEAVKYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDA 236

Query: 202 GRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHC 261
           GRSD+EFLYQILGEVI+AGATTLNI DTVGYT+P E+GKLIADIK NTPG +NVIISTHC
Sbjct: 237 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHC 296

Query: 262 QNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLEE-------------------- 321
           QNDLGLATANT+AGAC GARQVEVTINGIGE+AGNASLEE                    
Sbjct: 297 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGI 356

Query: 322 -----------VEEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGY 381
                      VEEYTGLNVQPHKAIVGANAFAH+SGIHQDGMLKH+GTYEII+PEDIGY
Sbjct: 357 NSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIIAPEDIGY 416

Query: 382 QRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQK 400
           +RSN   IVLGKLSGRHALKS L +LGYE+D +KLDNVFWRFKA+AEQK
Sbjct: 417 ERSNDAGIVLGKLSGRHALKSRLVELGYELDNEKLDNVFWRFKAVAEQK 465

[Close]

BLAST of CmoCh14G005770 vs. ExPASy TrEMBL
Match: A0A6J1G9D1 (2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111452159 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 4.3e-167
Identity = 303/409 (74.08%), Postives = 333/409 (81.42%), Query Frame = 0


Query: 22  CISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIA 81
           C     + S    +S  +RPPY PN I D+ YVR+FDTTLRDGEQSPG +LT K+KL IA
Sbjct: 57  CSHTESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIA 116

Query: 82  RQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCTESDVRAA 141
           RQLVKL VD IEAGFPAAS++DFEAV+M+A EVGN V EDGYVPVICG SRC E+D+R A
Sbjct: 117 RQLVKLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVNEDGYVPVICGLSRCNENDIRTA 176

Query: 142 WEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDA 201
           WEAVKYAKRPRIH FIATSEIH+ HKLRKTR +V+EIARNMVRFARSLG DDVEFSPEDA
Sbjct: 177 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 236

Query: 202 GRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHC 261
           GRSD+EFLYQILGEVI+AGATTLNI DTVGYT+P E+G LIADIK NTPG  NVIISTHC
Sbjct: 237 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHC 296

Query: 262 QNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLEE-------------------- 321
           QNDLGLATANT+AGAC GARQ+EVTINGIGE+AGNASLEE                    
Sbjct: 297 QNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGI 356

Query: 322 -----------VEEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGY 381
                      VEEYTGLNVQPHKAIVGANAFAH+SGIHQDGMLKH+GTYEIISPEDIGY
Sbjct: 357 NSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGY 416

Query: 382 QRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQK 400
           +RSN   IVLGKLSGRHALKS L +LGYE+D + LDNVFWRFKA+AEQK
Sbjct: 417 ERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQK 465

[Close]

BLAST of CmoCh14G005770 vs. ExPASy TrEMBL
Match: A0A6J1CL37 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.8e-166
Identity = 306/421 (72.68%), Postives = 338/421 (80.29%), Query Frame = 0


Query: 13  SMSKPNHDVCISRHQ---ITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTTLRDGEQSPG 72
           S   P   VC S  Q   I S    +S  QRPPY PN I D  YVR+FDTTLRDGEQSPG
Sbjct: 38  SFRSPAGRVCCSEAQSISIASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPG 97

Query: 73  TALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGEDGYVPVICG 132
            +LT K+KL IARQL KL VD IEAGFPAAS++DFEAV+++AKEVGN V +DGYVPVICG
Sbjct: 98  ASLTSKEKLDIARQLAKLGVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICG 157

Query: 133 FSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSL 192
            SRC E D+R AWEAVKYAKRPRIH FIATSEIH+ +KLRKTR EV+EIAR+MVRFARSL
Sbjct: 158 LSRCNEKDIRTAWEAVKYAKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSL 217

Query: 193 GFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNT 252
           G DDVEFSPEDAGRSD+EFLY+ILGEVI+AGATTLNI DTVGYT+P E+G LIADIK NT
Sbjct: 218 GCDDVEFSPEDAGRSDREFLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNT 277

Query: 253 PGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLEE-------- 312
           PG +NVIISTHCQNDLGLATANT+AGAC GARQVEVTINGIGE+AGNASLEE        
Sbjct: 278 PGIENVIISTHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCR 337

Query: 313 -----------------------VEEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRG 372
                                  VEEYTGLNVQPHKAIVGANAFAH+SGIHQDGMLKH+G
Sbjct: 338 GEHVLGGLHSGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKG 397

Query: 373 TYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQ 400
           TYEIISPEDIGY+RSN   IVLGKLSGRHALKS L++LGYE+D + LDN+FWRFKA+AEQ
Sbjct: 398 TYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVAEQ 457

[Close]

BLAST of CmoCh14G005770 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 547.0 bits (1408), Expect = 1.3e-155
Identity = 275/393 (69.97%), Postives = 317/393 (80.66%), Query Frame = 0


Query: 38  RQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFP 97
           R+RP Y PN I D  YVR+FDTTLRDGEQSPG  LT K+KL IARQL KL VD IEAGFP
Sbjct: 71  RRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFP 130

Query: 98  AASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFI 157
           AAS+ DFEAV+ +A+ VGN V E+GYVPVICG SRC + D+  AWEAVKYAKRPRIH FI
Sbjct: 131 AASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFI 190

Query: 158 ATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVI 217
           ATS+IHL +KL+K++ EV+EIARNMVRFARSLG +DVEFSPEDAGRS++E+LY+ILGEVI
Sbjct: 191 ATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVI 250

Query: 218 RAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGAC 277
           +AGATTLNI DTVG T+P+E+G+LIADIK NTPG QNVIISTHCQNDLGL+TANT++GA 
Sbjct: 251 KAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAH 310

Query: 278 GGARQVEVTINGIGEKAGNASLEE-------------------------------VEEYT 337
            GARQVEVTINGIGE+AGNASLEE                               VEEYT
Sbjct: 311 SGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYT 370

Query: 338 GLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGR 397
           G+  QPHKAIVGANAFAH+SGIHQDGMLKH+GTYEI+SPE+IG +RSN   IVLGKLSGR
Sbjct: 371 GMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGR 430

Query: 398 HALKSYLRKLGYEIDEDKLDNVFWRFKALAEQK 400
           HALK  L +LGY +D+ +L N+FWRFKA+AEQK
Sbjct: 431 HALKDRLNELGYVLDDGQLSNLFWRFKAVAEQK 463

[Close]

BLAST of CmoCh14G005770 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 538.1 bits (1385), Expect = 6.0e-153
Identity = 278/435 (63.91%), Postives = 329/435 (75.63%), Query Frame = 0


Query: 7   PVSMSMSMSKPNHDVCISRHQIT---SCHVV--------SSVRQRPPYTPNSILDAPYVR 66
           P+S S    +P+H   IS    T   SC +         +  R RP Y PN I D  YVR
Sbjct: 32  PISSSFRF-QPSHHRSISLRSQTLRLSCSISDPSPLPPHTPRRPRPEYIPNRISDPNYVR 91

Query: 67  VFDTTLRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVG 126
           VFDTTLRDGEQSPG  LT K+KL IARQL KL VD IEAGFPAAS+ DFEAV+ +A+ VG
Sbjct: 92  VFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVG 151

Query: 127 NEVGEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEV 186
           N V E+GYVPVICG SRC + D+  AW+AVKYAKRPRIH FIATS+IHL +KL+KT+ EV
Sbjct: 152 NTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKKTKAEV 211

Query: 187 VEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVP 246
           +EIAR+MVRFARSLG +DVEFSPEDAGRS++E+LY+ILGEVI+AGATTLNI DTVG T+P
Sbjct: 212 IEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLP 271

Query: 247 AEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAG 306
           +E+G+LI D+K NTPG +NV+ISTHCQNDLGL+TANT++GA  GARQ+EVTINGIGE+AG
Sbjct: 272 SEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGARQMEVTINGIGERAG 331

Query: 307 NASLEE-------------------------------VEEYTGLNVQPHKAIVGANAFAH 366
           NASLEE                               VEEYTG+  QPHKAIVGANAFAH
Sbjct: 332 NASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAH 391

Query: 367 QSGIHQDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDK 400
           +SGIHQDGMLKH+GTYEII PE+IG +RSN   IVLGKLSGRHALK  L +LGY++D+++
Sbjct: 392 ESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLDDEQ 451

[Close]

BLAST of CmoCh14G005770 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 450.3 bits (1157), Expect = 1.6e-126
Identity = 231/413 (55.93%), Postives = 296/413 (71.67%), Query Frame = 0


Query: 23  ISRHQITSCHVVSSVRQR-PPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIA 82
           +S++  TS   +  V +R P Y PN + D  YVRVFDTTLRDGEQSPG +LT  QKL IA
Sbjct: 53  VSKNAATSSTDLKPVVERWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLTPPQKLEIA 112

Query: 83  RQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGED-GYVPVICGFSRCTESDVRA 142
           RQL KLRVD +E GFP +S ++ E ++ +AK VGNEV E+ GYVPVIC  +RC   D+ A
Sbjct: 113 RQLAKLRVDIMEVGFPGSSEEELETIKTIAKTVGNEVDEETGYVPVICAIARCKHRDIEA 172

Query: 143 AWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPED 202
            WEA+KYAKRPRI +F +TS+IH+ +KL+KT+ EV+E+A + +RFA+SLGF+D++F  ED
Sbjct: 173 TWEALKYAKRPRILVFTSTSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFNDIQFGCED 232

Query: 203 AGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTH 262
            GRSDK+FL +ILGE I+AG T + I DTVG  +P EYG+L+  +K NTPG  +V+++ H
Sbjct: 233 GGRSDKDFLCKILGEAIKAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGIDDVVVAVH 292

Query: 263 CQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLEE------------------- 322
           C NDLGLATAN+IAG   GARQVEVTINGIGE++GNASLEE                   
Sbjct: 293 CHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEEVVMALKCRGAYVINGVYTK 352

Query: 323 ------------VEEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIG 382
                       V+EYTGL VQ HK IVGAN F H+SGIHQDG+LK+R TYEI+SPEDIG
Sbjct: 353 IDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILSPEDIG 412

Query: 383 YQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQKNVI 403
             +S +  +VLGKLSGRHA+K  L++LGYE+D++KL+ VF  F+ L + K  I
Sbjct: 413 IVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLFRDLTKNKKRI 465

[Close]

BLAST of CmoCh14G005770 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 447.6 bits (1150), Expect = 1.1e-125
Identity = 220/398 (55.28%), Postives = 290/398 (72.86%), Query Frame = 0


Query: 37  VRQRPPYTPNSILDAPYVRVFDTTLRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGF 96
           + +RP Y PN +    YVRV DTTLRDGEQSPG ALT  QKL IARQL KLRVD +E GF
Sbjct: 68  MERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGF 127

Query: 97  PAASRQDFEAVEMVAKEVGNEVGED-GYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHM 156
           P +S ++FEA++ +AK VGNEV E+ GYVPVICG +RC + D+ A WEA+KYAKRPR+ +
Sbjct: 128 PVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVML 187

Query: 157 FIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGE 216
           F +TSEIH+ +KL+KT+ EV+E+A N V++A+SLGF D++F  ED GR++K+F+ +ILGE
Sbjct: 188 FTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILGE 247

Query: 217 VIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTIAG 276
            I+AGATT+   DTVG  +P E+G+L+A +  NTPG  +++ + HC NDLG+ATANTI+G
Sbjct: 248 SIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDIVFAIHCHNDLGVATANTISG 307

Query: 277 ACGGARQVEVTINGIGEKAGNASLEE-------------------------------VEE 336
            C GARQVEVTINGIGE++GNA LEE                               V+E
Sbjct: 308 ICAGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQE 367

Query: 337 YTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLS 396
           +TG+ VQPHK IVG N F H+SGIHQDG+LK+R TYEI+SPED+G  +S +  IVLGKLS
Sbjct: 368 HTGMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLS 427

Query: 397 GRHALKSYLRKLGYEIDEDKLDNVFWRFKALAEQKNVI 403
           GRHA+K  L++LGYEI ++K +++F R++ L + K  I
Sbjct: 428 GRHAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRI 465

The following BLAST results are available for this feature:

BLAST of CmoCh14G005770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C550	1.8e-154	69.97	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	8.4e-152	63.91	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04973	7.1e-151	65.04	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	9.4e-143	62.77	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q39891	4.8e-139	63.20	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of CmoCh14G005770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F8D9	5.6e-223	92.84	2-isopropylmalate synthase 2, chloroplastic-like isoform X2 OS=Cucurbita moschat...	[more]
A0A6J1F3I8	6.2e-222	92.81	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 OS=Cucurbita moschat...	[more]
A0A6J1J7V5	1.1e-167	74.08	2-isopropylmalate synthase OS=Cucurbita maxima OX=3661 GN=LOC111482524 PE=3 SV=1	[more]
A0A6J1G9D1	4.3e-167	74.08	2-isopropylmalate synthase OS=Cucurbita moschata OX=3662 GN=LOC111452159 PE=3 SV...	[more]
A0A6J1CL37	2.8e-166	72.68	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]

BLAST of CmoCh14G005770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74040.1	1.3e-155	69.97	2-isopropylmalate synthase 1	[more]
AT1G18500.1	6.0e-153	63.91	methylthioalkylmalate synthase-like 4	[more]
AT5G23010.1	1.6e-126	55.93	methylthioalkylmalate synthase 1	[more]
AT5G23020.1	1.1e-125	55.28	2-isopropylmalate synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 49..310 e-value: 3.5E-84 score: 284.8
None	No IPR available	GENE3D	1.10.238.260		coord: 311..402 e-value: 2.0E-34 score: 120.3
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 302..400 coord: 41..302
None	No IPR available	CDD	cd07940	DRE_TIM_IPMS	coord: 56..302 e-value: 1.38885E-150 score: 424.939
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 52..320
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 54..302 e-value: 1.1E-82 score: 277.5
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 54..323 score: 34.943836
IPR002034	Alpha-isopropylmalate/homocitrate synthase, conserved site	PROSITE	PS00815	AIPM_HOMOCIT_SYNTH_1	coord: 61..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh14G005770.1	CmoCh14G005770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0046912	acyltransferase, acyl groups converted into alkyl on transfer
molecular_function	GO:0003824	catalytic activity