CmoCh14G001170 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh14G001170
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	Cmo_Chr14: 526856 .. 528288 (-)
RNA-Seq Expression	CmoCh14G001170
Synteny	CmoCh14G001170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTAGCATGTCGCCATGGACAGTAAGATTTGAACAACACACTCATATAGTACCCTCGTGGAAGGAAGAAGGAGGACGACATAAATTGTGAGAATCTTTATCTCTCCATATTTTAATATTATCTTTTCAATCTCTTCAAATATTCATTTCATTCTTCAATTCGGTGCAGATGAATTCCCCGATTCAAAGATGGTCTCTCAATGGCTTCACCGCCCTCGTCACCGGCGGAACCCGAGGCATTGGGTCGGCCTCTAATCATTATCAATCCCACTTCTCATATCATTCATCTATTTATGTTCTTACTTGTTTTGTTTTATTTTGTTTTCAGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTAACCAAACAGAGCTGGACCAAAGGGTAAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCAGCGACAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTAATTCTCTCTCTCATTTATTTTCTTTACAAACCCAAAATGCTCTAATTCTGAAAATGGGTGGTTGGTTTTAAGGTGAACAACGCAGGGGCGGTAACACTGAAATCAGCAGCAGAGCACACAACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCAGGAGTCGTCGCTTTACCAAAAATATCAATCTATGCGGCATCTAAAGGTATGGGGTTAGATAAATCTGTGGGTAATGAATGATTGGAAGATTCAAGATTGTTCTTTGACGGTTTGTGGGTTTGTACTTCGATTGCAGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCAAAGGATAATATTCGTATCAACACTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAGGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCTGACGCCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTGGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACGTGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAGACTTTAGTATGGGCTGTGCATTTGTGTGGTGTAATGTAGCAATGTTTCAGTTTATGTGTATCATTGAAAGTGGTCAAACCCAGAAAGTAAAACGTTAAACAGTAGGCACAGTGTCTCAGTCTGAAAGAAAATAAAATGTATAATGTCGGCCATGTGATGTGACAAGGTCCTCCTTTGGGCTTTCATTTTCTATTTTAAGTTTTGGACAAAAGATGAATTAATATCATATCCTCC

mRNA sequence

AACTAGCATGTCGCCATGGACAGTAAGATTTGAACAACACACTCATATAGTACCCTCGTGGAAGGAAGAAGGAGGACGACATAAATTATGAATTCCCCGATTCAAAGATGGTCTCTCAATGGCTTCACCGCCCTCGTCACCGGCGGAACCCGAGGCATTGGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTAACCAAACAGAGCTGGACCAAAGGGTAAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCAGCGACAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTGAACAACGCAGGGGCGGTAACACTGAAATCAGCAGCAGAGCACACAACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCAGGAGTCGTCGCTTTACCAAAAATATCAATCTATGCGGCATCTAAAGGTATGGGGTTAGATAAATCTGTGGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCAAAGGATAATATTCGTATCAACACTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAGGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCTGACGCCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTGGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACGTGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAGACTTTAGTATGGGCTGTGCATTTGTGTGGTGTAATGTAGCAATGTTTCAGTTTATGTGTATCATTGAAAGTGGTCAAACCCAGAAAGTAAAACGTTAAACAGTAGGCACAGTGTCTCAGTCTGAAAGAAAATAAAATGTATAATGTCGGCCATGTGATGTGACAAGGTCCTCCTTTGGGCTTTCATTTTCTATTTTAAGTTTTGGACAAAAGATGAATTAATATCATATCCTCC

Coding sequence (CDS)

ATGAATTCCCCGATTCAAAGATGGTCTCTCAATGGCTTCACCGCCCTCGTCACCGGCGGAACCCGAGGCATTGGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTAACCAAACAGAGCTGGACCAAAGGGTAAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCAGCGACAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTGAACAACGCAGGGGCGGTAACACTGAAATCAGCAGCAGAGCACACAACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCAGGAGTCGTCGCTTTACCAAAAATATCAATCTATGCGGCATCTAAAGGTATGGGGTTAGATAAATCTGTGGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCAAAGGATAATATTCGTATCAACACTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAGGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCTGACGCCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTGGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACGTGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAG

Protein sequence

MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLPAASYVTGQVICVDGGYTAGGG

Homology

[Close]

BLAST of CmoCh14G001170 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 4.2e-83
Identity = 160/264 (60.61%), Postives = 195/264 (73.86%), Query Frame = 0


Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G TALVTGGTRGIGYAIVEELA+ GA V+TCSR+Q +LD+ +++W RKGF+VSG
Sbjct: 11  RRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVSG 70

Query: 66  SVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAP 125
            VCD+SS SQRQ L+ +V+S FNGKLNIL+NNAG    K A   T EDY+ IM TNFEA 
Sbjct: 71  PVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIPKEATNFTAEDYSIIMGTNFEAS 130

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLA 185
           Y+L Q+++P LKASG  SIVF SS AGV+A+P  SIYAASK        GAINQ+TK+LA
Sbjct: 131 YNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASK--------GAINQVTKSLA 190

Query: 186 CEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLEL---TPAGRIGEANEISSVVAF 245
           CEWAKD+IR+N VAPW + T I      V  +   +  L    P  R GE +E+SS+V +
Sbjct: 191 CEWAKDSIRVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTY 250

Query: 246 LCLPAASYVTGQVICVDGGYTAGG 267
           LCLP ASY+TGQ+ICVDGGYT  G
Sbjct: 251 LCLPTASYITGQIICVDGGYTVNG 266

[Close]

BLAST of CmoCh14G001170 vs. ExPASy Swiss-Prot
Match: P50162 (Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.0e-81
Identity = 156/265 (58.87%), Postives = 197/265 (74.34%), Query Frame = 0


Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++GIGYAIVEELA LGA V+TCSRN+ ELD+ ++ W  KG  V GS
Sbjct: 16  RWSLKGTTALVTGGSKGIGYAIVEELAGLGARVYTCSRNEKELDECLEIWREKGLNVEGS 75

Query: 67  VCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCDL SR++R +L+ TV+ +F+GKLNILVNNAG V  K A + T +DYN IM TNFEA Y
Sbjct: 76  VCDLLSRTERDKLMQTVAHVFDGKLNILVNNAGVVIHKEAKDFTEKDYNIIMGTNFEAAY 135

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLAC 186
           HLSQI+YP LKAS  G+++F+SS+AG  ALP +S+Y+ASK        GAINQ+TK+LAC
Sbjct: 136 HLSQIAYPLLKASQNGNVIFLSSIAGFSALPSVSLYSASK--------GAINQMTKSLAC 195

Query: 187 EWAKDNIRINTVAPWAVKT----TISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAF 246
           EWAKDNIR+N+VAP  + T    T  + +    EE    +  TP GR G+  E+S+++AF
Sbjct: 196 EWAKDNIRVNSVAPGVILTPLVETAIKKNPHQKEEIDNFIVKTPMGRAGKPQEVSALIAF 255

Query: 247 LCLPAASYVTGQVICVDGGYTAGGG 268
           LC PAASY+TGQ+I  DGG+TA GG
Sbjct: 256 LCFPAASYITGQIIWADGGFTANGG 272

[Close]

BLAST of CmoCh14G001170 vs. ExPASy Swiss-Prot
Match: Q9ZW04 (Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g29170 PE=3 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 5.2e-81
Identity = 151/257 (58.75%), Postives = 195/257 (75.88%), Query Frame = 0


Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+RN+T+L + V+EW+ KGFEV+ S
Sbjct: 13  RWSLGGMTALVTGGSKGLGEAVVEELAMLGARVHTCARNETQLQECVREWQAKGFEVTTS 72

Query: 67  VCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR QR++L+  V+SIF GKLNILVNNAG    K   E+T +DY+ +M+TN ++ +
Sbjct: 73  VCDVSSRDQREKLMENVASIFQGKLNILVNNAGTGITKPTTEYTAQDYSFLMATNLDSAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLAC 186
           HLSQ+++P LKASG GSIV +SS AGVV +   SIY A+K        GA+NQ+ KNLAC
Sbjct: 133 HLSQLAHPLLKASGSGSIVLMSSTAGVVHINVGSIYGATK--------GAMNQLAKNLAC 192

Query: 187 EWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLP 246
           EWA+DNIR+N+V PW + T +   D  + +E  R    TP GR+G ANE+SS+VAFLC P
Sbjct: 193 EWARDNIRVNSVCPWFIATPLYLNDEELKKEVER---KTPMGRVGNANEVSSLVAFLCFP 252

Query: 247 AASYVTGQVICVDGGYT 264
           AASY+TGQ ICVDGG+T
Sbjct: 253 AASYITGQTICVDGGFT 258

[Close]

BLAST of CmoCh14G001170 vs. ExPASy Swiss-Prot
Match: Q9ZW03 (Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g29150 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.2e-80
Identity = 152/260 (58.46%), Postives = 200/260 (76.92%), Query Frame = 0


Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R++T+L +R++EW+ KGFEV+ S
Sbjct: 13  RWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTS 72

Query: 67  VCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR QR++L+ TVSS+F GKLNILVNNAG   +K + E+T EDY+ +M+TN E+ +
Sbjct: 73  VCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLAC 186
           HLSQI++P LKASG GSIVF+SSVAG+V     SIY ASK        GA+NQ+ ++LAC
Sbjct: 133 HLSQIAHPLLKASGSGSIVFMSSVAGLVH-TGASIYGASK--------GAMNQLGRSLAC 192

Query: 187 EWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLP 246
           EWA DNIR+N+V PW + T ++    +  +    + + TP GR+GEANE+SS+VAFLC P
Sbjct: 193 EWASDNIRVNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFP 252

Query: 247 AASYVTGQVICVDGGYTAGG 267
           AASY+TGQ ICVDGG +  G
Sbjct: 253 AASYITGQTICVDGGASVNG 263

[Close]

BLAST of CmoCh14G001170 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.5e-80
Identity = 147/261 (56.32%), Postives = 196/261 (75.10%), Query Frame = 0


Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R++T+L +R++EW+ KGF+V+ 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAP 125
           S+CD+S R QR++L+ TVSS+F GKLNILVNN G + LK   E+T E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLA 185
           +H+SQ+++P LKASG GSIV +SS+AGVV +   SIY A+K        GA+NQ+ +NLA
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATK--------GAMNQLARNLA 182

Query: 186 CEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCL 245
           CEWA DNIR N + PW + T +     +V E      E TP GR+GEANE+S +VAFLCL
Sbjct: 183 CEWASDNIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCL 242

Query: 246 PAASYVTGQVICVDGGYTAGG 267
           PAASY+TGQVICVDGG T  G
Sbjct: 243 PAASYITGQVICVDGGLTVNG 255

[Close]

BLAST of CmoCh14G001170 vs. ExPASy TrEMBL
Match: A0A6J1F1Z8 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.4e-137
Identity = 259/267 (97.00%), Postives = 259/267 (97.00%), Query Frame = 0


Query: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQI 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASK        GAINQI
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASK--------GAINQI 180

Query: 181 TKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVV 240
           TKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVV
Sbjct: 181 TKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVV 240

Query: 241 AFLCLPAASYVTGQVICVDGGYTAGGG 268
           AFLCLPAASYVTGQVICVDGGYTAGGG
Sbjct: 241 AFLCLPAASYVTGQVICVDGGYTAGGG 259

[Close]

BLAST of CmoCh14G001170 vs. ExPASy TrEMBL
Match: A0A6J1J921 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.6e-133
Identity = 249/267 (93.26%), Postives = 255/267 (95.51%), Query Frame = 0


Query: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS R+QLITTVSSIFNGKLNILVNNAG +TLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQI 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASK        GAINQI
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASK--------GAINQI 180

Query: 181 TKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVV 240
           TKNLACEWAKDNIRINTVAPWAVKTTIS+PDAAVVEEYSRLLE TPAGRIGEANEISSVV
Sbjct: 181 TKNLACEWAKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVV 240

Query: 241 AFLCLPAASYVTGQVICVDGGYTAGGG 268
           AFLCLPAASY+TGQVICVDGGYTAGGG
Sbjct: 241 AFLCLPAASYMTGQVICVDGGYTAGGG 259

[Close]

BLAST of CmoCh14G001170 vs. ExPASy TrEMBL
Match: A0A5D3DN35 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001440 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 7.2e-118
Identity = 218/266 (81.95%), Postives = 242/266 (90.98%), Query Frame = 0


Query: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SP+QRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S SQRQQLI TVSSIFNG LNILVNNAG VTLK A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQI 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALPKISIYAA+K        GAINQI
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATK--------GAINQI 180

Query: 181 TKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVV 240
           TKNLACEWAKDNIR+NTVAPW V+TTIS+PDAAVVEEY RL+  TPAGRIGE  EISSVV
Sbjct: 181 TKNLACEWAKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVV 240

Query: 241 AFLCLPAASYVTGQVICVDGGYTAGG 267
           AFLCLPAASYV+GQ+ICVDGG+TAGG
Sbjct: 241 AFLCLPAASYVSGQIICVDGGFTAGG 258

[Close]

BLAST of CmoCh14G001170 vs. ExPASy TrEMBL
Match: A0A1S3B6I2 (tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 7.2e-118
Identity = 218/266 (81.95%), Postives = 242/266 (90.98%), Query Frame = 0


Query: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SP+QRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S SQRQQLI TVSSIFNG LNILVNNAG VTLK A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQI 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALPKISIYAA+K        GAINQI
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATK--------GAINQI 180

Query: 181 TKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVV 240
           TKNLACEWAKDNIR+NTVAPW V+TTIS+PDAAVVEEY RL+  TPAGRIGE  EISSVV
Sbjct: 181 TKNLACEWAKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVV 240

Query: 241 AFLCLPAASYVTGQVICVDGGYTAGG 267
           AFLCLPAASYV+GQ+ICVDGG+TAGG
Sbjct: 241 AFLCLPAASYVSGQIICVDGGFTAGG 258

[Close]

BLAST of CmoCh14G001170 vs. ExPASy TrEMBL
Match: A0A0A0LAG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.6e-117
Identity = 217/266 (81.58%), Postives = 241/266 (90.60%), Query Frame = 0


Query: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SP+QRWSLNGFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S SQRQQLI TVSSIFNG LNILVNNAG VT+K+A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTMKTATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQI 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALP+ISIYAA+K        GAINQI
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPRISIYAATK--------GAINQI 180

Query: 181 TKNLACEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVV 240
           TKNLACEWAKDNIRINTVAPW V+TTIS PDAA VEEY RL+  TPAGR+GE  EISSVV
Sbjct: 181 TKNLACEWAKDNIRINTVAPWGVRTTISTPDAAAVEEYGRLIGRTPAGRLGEPEEISSVV 240

Query: 241 AFLCLPAASYVTGQVICVDGGYTAGG 267
           AFLCLPAASYV+GQ+ICVDGGYTAGG
Sbjct: 241 AFLCLPAASYVSGQIICVDGGYTAGG 258

[Close]

BLAST of CmoCh14G001170 vs. TAIR 10
Match: AT2G29150.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 8.2e-82
Identity = 152/260 (58.46%), Postives = 200/260 (76.92%), Query Frame = 0


Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R++T+L +R++EW+ KGFEV+ S
Sbjct: 13  RWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTS 72

Query: 67  VCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR QR++L+ TVSS+F GKLNILVNNAG   +K + E+T EDY+ +M+TN E+ +
Sbjct: 73  VCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLAC 186
           HLSQI++P LKASG GSIVF+SSVAG+V     SIY ASK        GA+NQ+ ++LAC
Sbjct: 133 HLSQIAHPLLKASGSGSIVFMSSVAGLVH-TGASIYGASK--------GAMNQLGRSLAC 192

Query: 187 EWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLP 246
           EWA DNIR+N+V PW + T ++    +  +    + + TP GR+GEANE+SS+VAFLC P
Sbjct: 193 EWASDNIRVNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFP 252

Query: 247 AASYVTGQVICVDGGYTAGG 267
           AASY+TGQ ICVDGG +  G
Sbjct: 253 AASYITGQTICVDGGASVNG 263

[Close]

BLAST of CmoCh14G001170 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.1e-81
Identity = 147/261 (56.32%), Postives = 196/261 (75.10%), Query Frame = 0


Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R++T+L +R++EW+ KGF+V+ 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAP 125
           S+CD+S R QR++L+ TVSS+F GKLNILVNN G + LK   E+T E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLA 185
           +H+SQ+++P LKASG GSIV +SS+AGVV +   SIY A+K        GA+NQ+ +NLA
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATK--------GAMNQLARNLA 182

Query: 186 CEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCL 245
           CEWA DNIR N + PW + T +     +V E      E TP GR+GEANE+S +VAFLCL
Sbjct: 183 CEWASDNIRTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCL 242

Query: 246 PAASYVTGQVICVDGGYTAGG 267
           PAASY+TGQVICVDGG T  G
Sbjct: 243 PAASYITGQVICVDGGLTVNG 255

[Close]

BLAST of CmoCh14G001170 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 4.1e-81
Identity = 147/261 (56.32%), Postives = 194/261 (74.33%), Query Frame = 0


Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSLNG +ALVTGGTRGIG AIVEELA LGA VHTC+RN+ EL+  + +W R GF V+G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 66  SVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAP 125
           SVCD+S RSQR+ L+ TVSS+F GKL+ILVNN G    K   E T  +++ +MSTNFE+ 
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESV 183

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLA 185
           +HL Q++YP L+ S  GS+VF+SSV+G V+L  +S+ +++K        GAINQ+T++LA
Sbjct: 184 FHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTK--------GAINQLTRSLA 243

Query: 186 CEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCL 245
           CEWAKDNIRIN VAPW +KT++     +  E    +  +TP GR+GE  E+SS VAFLCL
Sbjct: 244 CEWAKDNIRINAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCL 303

Query: 246 PAASYVTGQVICVDGGYTAGG 267
           PA+SY+TGQ++CVDGG +  G
Sbjct: 304 PASSYITGQILCVDGGMSING 316

[Close]

BLAST of CmoCh14G001170 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 9.1e-81
Identity = 154/261 (59.00%), Postives = 189/261 (72.41%), Query Frame = 0


Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G TALVTGGTRGIG A+VEELA  GA VHTCSRNQ EL+  + +W+  G  VSG
Sbjct: 5   KRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSG 64

Query: 66  SVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAP 125
           SVCD S R QR++LI   SS F+GKLNIL+NN G    K   E+++E+Y  IMSTN E+ 
Sbjct: 65  SVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESA 124

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLA 185
           +HLSQI++P LKASG GSIVF+SSVAG+V L   SIY A+K        GA+NQ+T+NLA
Sbjct: 125 FHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATK--------GALNQLTRNLA 184

Query: 186 CEWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCL 245
           CEWA DNIR N VAPW +KT++        E    ++  TP GR+GE  E+SS+VAFLCL
Sbjct: 185 CEWASDNIRTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCL 244

Query: 246 PAASYVTGQVICVDGGYTAGG 267
           PA+SY+TGQVI VDGG+T  G
Sbjct: 245 PASSYITGQVISVDGGFTVNG 257

[Close]

BLAST of CmoCh14G001170 vs. TAIR 10
Match: AT2G29350.1 (senescence-associated gene 13 )

HSP 1 Score: 297.4 bits (760), Expect = 1.2e-80
Identity = 150/260 (57.69%), Postives = 193/260 (74.23%), Query Frame = 0


Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++GIG A+VEELA LGA VHTC+R++T+L +R++EW+ KGF+V+ S
Sbjct: 12  RWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLREWQAKGFQVTTS 71

Query: 67  VCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR QR +L+ TVSS++ GKLNILVNN G    K   E+T ED++ +M+TN E+ +
Sbjct: 72  VCDVSSRDQRVKLMETVSSLYQGKLNILVNNVGTSIFKPTTEYTAEDFSFVMATNLESAF 131

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGMGLDKSVGAINQITKNLAC 186
           HLSQ+++P LKASG GSIV +SS AGVV +   SIY A+K        GA+NQ+ +NLAC
Sbjct: 132 HLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATK--------GAMNQLARNLAC 191

Query: 187 EWAKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLP 246
           EWA DNIR N+V PW + T +S  D    E     +  TP GR+GEANE+S +VAFLCLP
Sbjct: 192 EWASDNIRTNSVCPWYITTPLSN-DFFDEEFKKEAVRTTPMGRVGEANEVSPLVAFLCLP 251

Query: 247 AASYVTGQVICVDGGYTAGG 267
           +ASY+TGQ ICVDGG T  G
Sbjct: 252 SASYITGQTICVDGGATVNG 262

The following BLAST results are available for this feature:

BLAST of CmoCh14G001170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50165	4.2e-83	60.61	Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
P50162	1.0e-81	58.87	Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1	[more]
Q9ZW04	5.2e-81	58.75	Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g291...	[more]
Q9ZW03	1.2e-80	58.46	Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g291...	[more]
Q9ZW13	1.5e-80	56.32	Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292...	[more]

BLAST of CmoCh14G001170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1Z8	2.4e-137	97.00	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 S...	[more]
A0A6J1J921	4.6e-133	93.26	tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=...	[more]
A0A5D3DN35	7.2e-118	81.95	Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B6I2	7.2e-118	81.95	tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1	[more]
A0A0A0LAG7	1.6e-117	81.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1	[more]

BLAST of CmoCh14G001170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29150.1	8.2e-82	58.46	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29290.2	1.1e-81	56.32	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29260.1	4.1e-81	56.32	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G06060.1	9.1e-81	59.00	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29350.1	1.2e-80	57.69	senescence-associated gene 13	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 226..246 score: 32.66 coord: 191..208 score: 34.07 coord: 89..100 score: 58.29 coord: 14..31 score: 50.59 coord: 170..189 score: 27.28 coord: 136..152 score: 35.68
None	No IPR available	GENE3D	3.40.50.720		coord: 2..267 e-value: 4.0E-77 score: 260.9
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 21..263 e-value: 9.9E-52 score: 175.8
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 10..263 e-value: 1.4E-15 score: 55.0
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 16..207 e-value: 9.5E-17 score: 58.9
None	No IPR available	PANTHER	PTHR42898:SF28	TROPINONE REDUCTASE-LIKE PROTEIN	coord: 5..266
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 5..266
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 14..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh14G001170.1	CmoCh14G001170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity