CmoCh13G003750 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh13G003750
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	secoisolariciresinol dehydrogenase-like
Location	Cmo_Chr13: 4763337 .. 4764933 (-)
RNA-Seq Expression	CmoCh13G003750
Synteny	CmoCh13G003750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTAATTTTAATCGGTCACTTGAATATCTACGACCACTTCACTATTTGGTGCACTACTAGGCTAGAAGGCAAAGTGGCAGTAATCACAGGTGCGGCCAGCGGAATTTGGGAGTCCACAGCAAAACTCTTCTTTAAGCATGGAGCCAAAGTGGTGCTTGCAGACATCCTACACGATGTCGGTCACTCTCTTTCCCGCCATCTCTGTTCTTCGTCATCTTCATTCGTCCACTGCGATGTAACGAAAGAGAAAGAGATTGAAGATGCGGTTAACACAGCTATTTCTAAGTATGGAAAGTTAGACATCATGTTGAACAGCGCAGGCATTTCTAGAACTCTAAAATTCAACATTCTTGAAAACGAATTATCAAAGTTGAAGCCAGAAGATATAGCCGAAGCTGCTTTGTATTTAGCAAGTGATGCATCGAGATTTGTGAGTGGACATAACTTAATTGTTGATGGAGGCTTCACCGCAACAAACCAAGGTCTTTATTCCTACCAATAATAAAGTTGAAACGAAGAACTACTTGTTTCAGACTTCAAGTTCTTTCCTAAACTTGATCATGAATTATGTCCTAATTTTATTTGTGTGGTTTTTGTCTCAATTGAGCTATGTCATATTTCTCAGGTTTAACATTATTATAAGAATATGAATTCTAATAATGCAATTTAATTTTTCGCTGCTTAATCTCTAATTTGGTTATTGTCTCAATCTCTAACTTGAGATAGCTTAGGTTTCCTCATTACGTAGAAGTACCGCCTAATGCTATATTAATTGATATGCTCACTTGAGTTGGAGCCTATAAAGTTATGAGATTGTAATCACGCCCTAATGGACGACAATGTCCTCATAATGACCTTTAAACCTTCGACAGGATGAAATGTCACTAAAAAAAAGGTGGGTAGCTCTAGAAGCATCCCAAGAATTAAAAACACGTTATTGGACATGAAATTCGAGGAAGTGTTCCCAAGAGAATGATAAGTTTTGGTAGACTTCACCATCCACAAGAGGATGTTCGTGAGTGGACACCTGTAGTCGCAGTTCAGTACAGTTATCACCGAACAACGTAGTAATTTAAGGAAAAGGGACAAGAGGATGTCCAACCGTGAAAATCAAGAGTAAAAAACTTTCATGGCCAAAATTTGTTAAGGAAGGGAGAATTTGTAATACTCGAGCCATAGCGGGATTAAATACCTAATTTTTTTTTCAAGCTCAAACTTTTGGGATTTTTATCTTATGAACCAGAAACCGAACCGAGTCACTTCGGGTTCAGAAAAAACAACTCAACCCAACCCAACCCTTACAATTCGGATTGGGTTGATCCAAGTTGTTGAGTTGTTGGATTGGATGTAATCACGAGTGGATTGGTATGTGAACTGAGTCGTCACTGCCAAAAGTCGAATTTGGTGGGCGACATTGCTGTTAGTAACTGTTGCAAAACATGAACCACTCACTCACACACTACCTTTCATTTCCTTCATTGTCCCACAAATGTCAAAATGATTACTTTGTTCTTGTCTTCTTTTTATAAATAAAATCCCTTTTCTTTTTTGTTTTTTTTTTTTTTCCTTTTTTGAAAATATTTTAGATGTTTAA

mRNA sequence

ATGCTTAATTTTAATCGGCTAGAAGGCAAAGTGGCAGTAATCACAGGTGCGGCCAGCGGAATTTGGGAGTCCACAGCAAAACTCTTCTTTAAGCATGGAGCCAAAGTGGTGCTTGCAGACATCCTACACGATGTCGGTCACTCTCTTTCCCGCCATCTCTGTTCTTCGTCATCTTCATTCGTCCACTGCGATGTAACGAAAGAGAAAGAGATTGAAGATGCGGTTAACACAGCTATTTCTAAGTATGGAAAGTTAGACATCATGTTGAACAGCGCAGGCATTTCTAGAACTCTAAAATTCAACATTCTTGAAAACGAATTATCAAAGTTGAAGCCAGAAGATATAGCCGAAGCTGCTTTGTATTTAGCAAGTGATGCATCGAGATTTGTGAGTGGACATAACTTAATTGTTGATGGAGGCTTCACCGCAACAAACCAAGATGTTTAA

Coding sequence (CDS)

ATGCTTAATTTTAATCGGCTAGAAGGCAAAGTGGCAGTAATCACAGGTGCGGCCAGCGGAATTTGGGAGTCCACAGCAAAACTCTTCTTTAAGCATGGAGCCAAAGTGGTGCTTGCAGACATCCTACACGATGTCGGTCACTCTCTTTCCCGCCATCTCTGTTCTTCGTCATCTTCATTCGTCCACTGCGATGTAACGAAAGAGAAAGAGATTGAAGATGCGGTTAACACAGCTATTTCTAAGTATGGAAAGTTAGACATCATGTTGAACAGCGCAGGCATTTCTAGAACTCTAAAATTCAACATTCTTGAAAACGAATTATCAAAGTTGAAGCCAGAAGATATAGCCGAAGCTGCTTTGTATTTAGCAAGTGATGCATCGAGATTTGTGAGTGGACATAACTTAATTGTTGATGGAGGCTTCACCGCAACAAACCAAGATGTTTAA

Protein sequence

MLNFNRLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDVTKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSKLKPEDIAEAALYLASDASRFVSGHNLIVDGGFTATNQDV

Homology

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BLAST of CmoCh13G003750 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 3.8e-25
Identity = 55/101 (54.46%), Postives = 79/101 (78.22%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVA+ITG ASGI E+TAKLF +HGAKV +AD+  ++GHS+   + +S+S+++HCDV
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCDV 73

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENE 107
           T E  +++AV+  +S YGKLDIM ++AGIS   +  I++NE
Sbjct: 74  TNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNE 114

HSP 2 Score: 56.2 bits (134), Expect = 3.6e-07
Identity = 29/69 (42.03%), Postives = 42/69 (60.87%), Query Frame = 0


Query: 77  TAISKYGKLDIMLNSAGISRTLKFNILENELSKLKPEDIAEAALYLASDASRFVSGHNLI 136
           + I    + + ++N AG  +  KFN+          ED+A AALYLASD +++VSGHNL 
Sbjct: 208 SGIKNEEEFENVINFAGNLKGPKFNV----------EDVANAALYLASDEAKYVSGHNLF 266

Query: 137 VDGGFTATN 146
           +DGGF+  N
Sbjct: 268 IDGGFSVCN 266

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BLAST of CmoCh13G003750 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.4e-22
Identity = 60/127 (47.24%), Postives = 84/127 (66.14%), Query Frame = 0


Query: 5   NRLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSS-SSFVHC 64
           NRL+ KVA+ITG A GI E+TAKLF ++GAKVV+ADI  D G  +  ++ S    SFVHC
Sbjct: 12  NRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHC 71

Query: 65  DVTKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILE--NELSKLKPEDIAEAALY 124
           DVTK++++ + V+T I+K+GKLDIM  + G+  T  ++ILE  NE  K   +     A  
Sbjct: 72  DVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFL 131

Query: 125 LASDASR 129
           +A  A+R
Sbjct: 132 VAKHAAR 138

HSP 2 Score: 50.8 bits (120), Expect = 1.5e-05
Identity = 21/36 (58.33%), Postives = 29/36 (80.56%), Query Frame = 0


Query: 110 LKPEDIAEAALYLASDASRFVSGHNLIVDGGFTATN 146
           L+ ED+A+A  YLA D S++VSG NL++DGG+T TN
Sbjct: 231 LRAEDVADAVAYLAGDESKYVSGLNLVIDGGYTRTN 266

[Close]

BLAST of CmoCh13G003750 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.8e-22
Identity = 60/127 (47.24%), Postives = 84/127 (66.14%), Query Frame = 0


Query: 5   NRLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSS-SSFVHC 64
           NRL+ KVA+ITG A GI E+TAKLF ++GAKVV+ADI  D G  +  ++ S    SFVHC
Sbjct: 12  NRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHC 71

Query: 65  DVTKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILE--NELSKLKPEDIAEAALY 124
           DVTK++++ + V+T I+K+GKLDIM  + G+  T  ++ILE  NE  K   +     A  
Sbjct: 72  DVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFL 131

Query: 125 LASDASR 129
           +A  A+R
Sbjct: 132 VAKHAAR 138

HSP 2 Score: 50.8 bits (120), Expect = 1.5e-05
Identity = 21/36 (58.33%), Postives = 29/36 (80.56%), Query Frame = 0


Query: 110 LKPEDIAEAALYLASDASRFVSGHNLIVDGGFTATN 146
           L+ ED+A+A  YLA D S++VSG NL++DGG+T TN
Sbjct: 231 LRAEDVADAVAYLAGDESKYVSGLNLVIDGGYTRTN 266

[Close]

BLAST of CmoCh13G003750 vs. ExPASy Swiss-Prot
Match: H9BFQ1 (Tropinone reductase-like 2 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 6.7e-22
Identity = 55/107 (51.40%), Postives = 79/107 (73.83%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVA+ITG ASGI   TA+LF ++GAKVV+ADI  D+G +L+  L    + ++HCDV
Sbjct: 10  RLEGKVAIITGGASGIGACTAELFHENGAKVVIADIQDDLGQALATKL-GGKACYIHCDV 69

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGI--SRTLKFNILENELSKL 111
           +KE E+ + V+T ++KYG+LDIM N+AGI   + L  +++E+E S L
Sbjct: 70  SKEDEVINLVDTTVAKYGRLDIMFNNAGIIEGQGLPVSVVESEKSDL 115

HSP 2 Score: 43.5 bits (101), Expect = 2.4e-03
Identity = 39/110 (35.45%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 49  LSRHLCSSSSSFVHCDVTKEKEIE-----DAVNTAISKYGKLDIMLNSAGISRTLKFNIL 108
           LS H  ++S S V C + K    E       VN  IS YG +  + N +G     +   +
Sbjct: 158 LSGHAYAASKSGV-CGLAKNLTPELGKYGIRVN-CISPYGLVTGVSNVSGEGEANR-EFV 217

Query: 109 ENELSK--------LKPEDIAEAALYLASDASRFVSGHNLIVDGGFTATN 146
           E  LS+        L+ + IA+AAL+LASD + +VSG N++VDGG++  N
Sbjct: 218 EAMLSELGTLSGQTLRADGIAKAALFLASDEAYYVSGINMVVDGGYSVVN 264

[Close]

BLAST of CmoCh13G003750 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 8.8e-22
Identity = 53/99 (53.54%), Postives = 72/99 (72.73%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           +L GKVAVITG ASGI   TA+LF KHGA+VV+ADI  ++G SL   L   +SS+VHCDV
Sbjct: 14  KLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCDV 73

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILE 105
           T E ++  AV+ A++++GKLD+M N+AG+S    F + E
Sbjct: 74  TNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSE 112

HSP 2 Score: 47.8 bits (112), Expect = 1.3e-04
Identity = 23/36 (63.89%), Postives = 27/36 (75.00%), Query Frame = 0


Query: 110 LKPEDIAEAALYLASDASRFVSGHNLIVDGGFTATN 146
           LK +DIA AAL+LASD  R+VSG NL VDGG +  N
Sbjct: 231 LKADDIAAAALFLASDDGRYVSGQNLRVDGGLSVVN 266

[Close]

BLAST of CmoCh13G003750 vs. ExPASy TrEMBL
Match: A0A6J1EHJ0 (secoisolariciresinol dehydrogenase-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.6e-50
Identity = 129/252 (51.19%), Postives = 135/252 (53.57%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAI  YGKLDI+LN+AG S+TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAIFNYGKLDIILNNAGFSKTLKFNILENELSNFQKVDNVNLLGLFLGT 131

Query: 126 ------------------------------------------------------------ 147
                                                                       
Sbjct: 132 KHAARVMIPARQGSIIMMASGCSIIGGIGPYAYMSSKHGLLGLMKNAMVHLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.0e-49
Identity = 130/252 (51.59%), Postives = 135/252 (53.57%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAA GI ESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAISKYGKLDIMLN+AGIS TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 126 ------------------------------------------------------------ 147
                                                                       
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.0e-49
Identity = 130/252 (51.59%), Postives = 135/252 (53.57%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAA GI ESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAISKYGKLDIMLN+AGIS TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 126 ------------------------------------------------------------ 147
                                                                       
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. ExPASy TrEMBL
Match: A0A6J1EPB9 (secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 5.1e-49
Identity = 129/251 (51.39%), Postives = 134/251 (53.39%), Query Frame = 0


Query: 7   LEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDVT 66
           LEGKVAVITGAA GI ESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDVT
Sbjct: 13  LEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDVT 72

Query: 67  KEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK----------------- 126
           KEK+IE+AVNTAISKYGKLDIMLN+AGIS TLKFNILENELS                  
Sbjct: 73  KEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGTK 132

Query: 127 ------------------------------------------------------------ 147
                                                                       
Sbjct: 133 HAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNCV 192

[Close]

BLAST of CmoCh13G003750 vs. ExPASy TrEMBL
Match: A0A6J1ELD0 (secoisolariciresinol dehydrogenase-like isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.5e-48
Identity = 126/252 (50.00%), Postives = 132/252 (52.38%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAI  YGKLDI+LN+AG S+TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAIFNYGKLDIILNNAGFSKTLKFNILENELSNFQKVDNVNLLGLFLGT 131

Query: 126 ------------------------------------------------------------ 147
                                                                       
Sbjct: 132 KHAARVMIPARQGSIIMMASGCSIIGGIGPYAYMSSKHGLLGLMKNAMVHLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. NCBI nr
Match: KAG6583703.1 (hypothetical protein SDJN03_19635, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 228.8 bits (582), Expect = 3.1e-56
Identity = 131/200 (65.50%), Postives = 136/200 (68.00%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEG+VAVITGAASGIWESTAKLFFKHGAK VLADIL DVGHSLSRHLCSSSSSFVHC+V
Sbjct: 12  RLEGEVAVITGAASGIWESTAKLFFKHGAKGVLADILDDVGHSLSRHLCSSSSSFVHCNV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAISKYGKLDIMLN+A ISRTLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNADISRTLKFNILENELSDFQKVLNVNLLGVFLGT 131

Query: 126 ------------------------------------------LKPEDIAEAALYLASDAS 148
                                                     L PEDIAEAALYLASDAS
Sbjct: 132 KHAAKAMIPARQVPSQMTRDLFKLEDGDEFSNVYSHFKCGDILWPEDIAEAALYLASDAS 191

[Close]

BLAST of CmoCh13G003750 vs. NCBI nr
Match: KAG6583702.1 (hypothetical protein SDJN03_19634, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 208.4 bits (529), Expect = 4.3e-50
Identity = 131/253 (51.78%), Postives = 136/253 (53.75%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAA GI ESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAISKYGKLDIMLN+AGIS TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 126 ------------------------------------------------------------ 148
                                                                       
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATIDLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. NCBI nr
Match: XP_022927567.1 (secoisolariciresinol dehydrogenase-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 207.6 bits (527), Expect = 7.3e-50
Identity = 129/252 (51.19%), Postives = 135/252 (53.57%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAI  YGKLDI+LN+AG S+TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAIFNYGKLDIILNNAGFSKTLKFNILENELSNFQKVDNVNLLGLFLGT 131

Query: 126 ------------------------------------------------------------ 147
                                                                       
Sbjct: 132 KHAARVMIPARQGSIIMMASGCSIIGGIGPYAYMSSKHGLLGLMKNAMVHLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. NCBI nr
Match: XP_022927564.1 (secoisolariciresinol dehydrogenase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 206.1 bits (523), Expect = 2.1e-49
Identity = 130/252 (51.59%), Postives = 135/252 (53.57%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAA GI ESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAISKYGKLDIMLN+AGIS TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 126 ------------------------------------------------------------ 147
                                                                       
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. NCBI nr
Match: XP_022927566.1 (secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 206.1 bits (523), Expect = 2.1e-49
Identity = 130/252 (51.59%), Postives = 135/252 (53.57%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGKVAVITGAA GI ESTAKLFFKHGAKVVLADIL DVGHSLSRHLCSSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSK---------------- 125
           TKEK+IE+AVNTAISKYGKLDIMLN+AGIS TLKFNILENELS                 
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 126 ------------------------------------------------------------ 147
                                                                       
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

[Close]

BLAST of CmoCh13G003750 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 2.6e-21
Identity = 49/90 (54.44%), Postives = 70/90 (77.78%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           +LEGKVAVITG ASGI ++TA+ F   GA+V++ DI  + GH ++  L  S++ F+ CDV
Sbjct: 35  KLEGKVAVITGGASGIGKATAEEFVSQGAQVIIVDIDEEAGHMVATEL-GSAAHFLRCDV 94

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGIS 96
           T+E++I  AV TA++++GKLD+MLNSAGIS
Sbjct: 95  TEEEQIAKAVETAVTRHGKLDVMLNSAGIS 123

HSP 2 Score: 52.4 bits (124), Expect = 3.7e-07
Identity = 23/34 (67.65%), Postives = 30/34 (88.24%), Query Frame = 0


Query: 109 KLKPEDIAEAALYLASDASRFVSGHNLIVDGGFT 143
           K +  D+A+AALYLASD ++FV+GHNL+VDGGFT
Sbjct: 257 KCEEIDVAKAALYLASDDAKFVTGHNLVVDGGFT 290

[Close]

BLAST of CmoCh13G003750 vs. TAIR 10
Match: AT2G47120.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 4.9e-20
Identity = 43/94 (45.74%), Postives = 64/94 (68.09%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RLEGK+ +ITG ASGI    A+LF  HGAKVV+ D+  ++G +++  +    +SF  CDV
Sbjct: 5   RLEGKIVIITGGASGIGADAARLFTDHGAKVVIVDVQEELGQNVAVLIGKDKASFYRCDV 64

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLK 100
           T E E+EDAV   + K+GKLD++ ++AG+   L+
Sbjct: 65  TNETEVEDAVKFTVEKHGKLDVLFSNAGVLEPLE 98

HSP 2 Score: 43.5 bits (101), Expect = 1.7e-04
Identity = 19/33 (57.58%), Postives = 25/33 (75.76%), Query Frame = 0


Query: 110 LKPEDIAEAALYLASDASRFVSGHNLIVDGGFT 143
           LK   +A+ AL+LASD S ++SG NL VDGG+T
Sbjct: 219 LKASHVAQVALFLASDDSAYISGQNLAVDGGYT 251

[Close]

BLAST of CmoCh13G003750 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 6.5e-20
Identity = 42/89 (47.19%), Postives = 62/89 (69.66%), Query Frame = 0


Query: 6  RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
          RL+GK+A+ITG ASGI     +LF  HGAKVV+ D   ++G +++  +    +SF  CDV
Sbjct: 5  RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRCDV 64

Query: 66 TKEKEIEDAVNTAISKYGKLDIMLNSAGI 95
          T EKE+E+AV   + KYGKLD++ ++AG+
Sbjct: 65 TNEKEVENAVKFTVEKYGKLDVLFSNAGV 93

HSP 2 Score: 45.1 bits (105), Expect = 5.9e-05
Identity = 21/33 (63.64%), Postives = 26/33 (78.79%), Query Frame = 0


Query: 110 LKPEDIAEAALYLASDASRFVSGHNLIVDGGFT 143
           LK   +AEAAL+LASD S +VSG NL VDGG++
Sbjct: 220 LKARHVAEAALFLASDDSAYVSGQNLAVDGGYS 252

[Close]

BLAST of CmoCh13G003750 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 8.4e-20
Identity = 43/105 (40.95%), Postives = 72/105 (68.57%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSSFVHCDV 65
           RL+GK+ +ITG ASGI   + +LF +HGA+VV+ D+  ++G +++  +    +S+ HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 66  TKEKEIEDAVNTAISKYGKLDIMLNSAGISRTLKFNILENELSKL 111
           T E E+E+AV   + KYGKLD++ ++AG+      +IL+  L++L
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNEL 108

[Close]

BLAST of CmoCh13G003750 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 1.9e-19
Identity = 45/93 (48.39%), Postives = 67/93 (72.04%), Query Frame = 0


Query: 6   RLEGKVAVITGAASGIWESTAKLFFKHGAKVVLADILHDVGHSLSRHLCSSSSS----FV 65
           RLEGKVA+ITG A GI ++T  LF +HGA VV+AD+ +  G SL++ L S  +S    F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 66  HCDVTKEKEIEDAVNTAISKYGKLDIMLNSAGI 95
            CDV+ E ++E+ VN  +++YG+LDI+ N+AG+
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGV 123

HSP 2 Score: 53.9 bits (128), Expect = 1.3e-07
Identity = 25/39 (64.10%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 110 LKPEDIAEAALYLASDASRFVSGHNLIVDGGFTATNQDV 149
           L+  DIAEAALYLASD S++V+GHNL+VDGG T     V
Sbjct: 263 LRANDIAEAALYLASDESKYVNGHNLVVDGGVTTARNCV 301

The following BLAST results are available for this feature:

BLAST of CmoCh13G003750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	3.8e-25	54.46	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q94KL8	1.4e-22	47.24	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	1.8e-22	47.24	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
H9BFQ1	6.7e-22	51.40	Tropinone reductase-like 2 OS=Erythroxylum coca OX=289672 PE=2 SV=1	[more]
Q7FAE1	8.8e-22	53.54	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]

BLAST of CmoCh13G003750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EHJ0	3.6e-50	51.19	secoisolariciresinol dehydrogenase-like isoform X4 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EID0	1.0e-49	51.59	secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EI14	1.0e-49	51.59	secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EPB9	5.1e-49	51.39	secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1ELD0	1.5e-48	50.00	secoisolariciresinol dehydrogenase-like isoform X5 OS=Cucurbita moschata OX=3662...	[more]

BLAST of CmoCh13G003750 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6583703.1	3.1e-56	65.50	hypothetical protein SDJN03_19635, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6583702.1	4.3e-50	51.78	hypothetical protein SDJN03_19634, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022927567.1	7.3e-50	51.19	secoisolariciresinol dehydrogenase-like isoform X4 [Cucurbita moschata]	[more]
XP_022927564.1	2.1e-49	51.59	secoisolariciresinol dehydrogenase-like isoform X1 [Cucurbita moschata]	[more]
XP_022927566.1	2.1e-49	51.59	secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata]	[more]

BLAST of CmoCh13G003750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26760.1	2.6e-21	54.44	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47120.1	4.9e-20	45.74	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47130.1	6.5e-20	47.19	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	8.4e-20	40.95	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	1.9e-19	48.39	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 83..94 score: 49.96 coord: 11..28 score: 42.1
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 10..105 e-value: 2.1E-19 score: 69.7
None	No IPR available	GENE3D	3.40.50.720		coord: 3..101 e-value: 1.0E-23 score: 85.7
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 110..142 e-value: 1.4E-9 score: 37.9
None	No IPR available	GENE3D	3.40.50.720		coord: 102..147 e-value: 1.5E-10 score: 42.7
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 6..95 e-value: 4.8E-6 score: 23.7
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 4..108 coord: 109..146
None	No IPR available	PANTHER	PTHR43180:SF30	MOMILACTONE A SYNTHASE	coord: 4..108
None	No IPR available	PANTHER	PTHR43180:SF30	MOMILACTONE A SYNTHASE	coord: 109..146
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh13G003750.1	CmoCh13G003750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity