CmoCh13G003730 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G003730
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionReverse transcriptase
LocationCmo_Chr13: 4747472 .. 4749430 (+)
RNA-Seq ExpressionCmoCh13G003730
SyntenyCmoCh13G003730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGTAGATGCCACAATAAACTCTCAACCGAGCAAGAGCACTCTGATAGACTCTGGAGCAACCCACAACTTCATTGCCGATCAAGAAGCCAGAAGATTGGGACTCACCATAGGAAAAGACCCGGGGAAAATGAAAGCTGTCAACTCCGAGGCCTTGCCTATTGTGGGAGTTTCCAAGAGAGTCCCCTTCAAAATAGGGGATTGGACTGGAGAGCTAGACCTTGTCGTGGTTCGCATGGACGACTTCGACGTGGTACTTGGGATGGAGTTCCTCCTAGAACACAAGGTCATCCCAATGCCATTGGCAAAATGCTTGGTGATCACCGACCGTAACCCCACAGTAATACATGCAAGCATCAAGCAACCAGGTAATCTTCGAATGATCTCGGCCATACAACTGAAAAGGGGACTCGCACGAGAGAAACCTACATTCATGGCCATACCACTGATAGAGGAAGCAACCACCGAAGAGACTGTCCCAGAGGAAATCAAGGAAGTACTAGACAGCTATACCGACATAATGCCAGAGAGTCTACCACAAACACTACCACCTCGTCGAGGCATTGACCACGAAATCAAACTCCTTCCCGGGGTTAAGCCGCCAGCAAAGAACGCATACCGGATGGCTCCGCCCGAGCTAGCTGAATTGAGGAAACAACTGGATGAGTTGTTGAAGGCGGGATTCATTCGCCCGGCAAAGGCCCCTTATGGAGCCCTCATACTGTTCCAGAAGAAGAAGGATGGGACGTTGCGTCTATGCATAGACTATAGAGCCTTAAACAAGGTGACAGTACGCAACAAATATCCTTTGCCGATAATATCCGACTTGTTCGACCAACTTCACGGGGCCAAGTACTTCACGAAGTTGGACTTACGATCAGGATATTATCAAGTACGTATTGCCGAAGGGGACGAACCTAAGACGACGTGCGTAACAAGATATGGGGCCTTTGAATTCCTAGTAATGCCCTTTGGTTTGACAAACGCCCCAGCTACGTTCTGCACGTTGATGAACCAGGTTTTCTATGAATACCTGGATCAGTTCGTCATAGTATACCTCGACGACATTGTTGTTTACAGCACAACCCTCGAGGAACACAAAGTGCATTTGAAGTTAGTGTTTGACAAGCTCCGGCAGAATCAGCTGTATGTGAAGAAGGAGAAATGTGCATTCGCACAAACATGCATCAACTTCCTTGGACATGTCGTCAGATGTGGACAAATTAGTATGGATAGCGACAAGATAAAAGCTATCCAAGAATGGAAGGTTCCTACTTCCGTATCTGAGTTGCGGTCCTTCTTAGGACTAGCCAACTACTATAGGCGGTTCGTCGAAGGGTTTTCACGACGAGCCGCCCCATTGACAGAGCTGTTGAAGAAAGACCACCCTTGGTCGTGGTCGAATGATTGTCAAATGGCCTTTGAAAATCTGAAAACAACCATGACGAGGGGTCCTGTCCTCGGGTTGGTAGACGTCACAAAGCCATTTGAAGTAGAAACCGATGCTTCGGACTTTGCTCTCGGTGGAGTCCTTATTCAAGAAGGCCACCCCATCACTTACGAAAGTCGAAAGCTCAACGATGCCGAACGAAGATACACTGTCTCCGAGAAAGAAATGCAGGCTGTAGTCCATTGCCTTCGAGTCTGGAGACAGTATCTCTTAGGATCACAGTTCGTAGTGAAGACGGATAACAGCGCCACTTGCTACTTCTTTGATCAACCAAAATTAATAGCAAAACAAGCCCGGTGGCAGGAGTCGTTGGCCGAATTCGACTTCAAGTTCGAACACAAAGCAGGAAAGAGCAATCAAGCAGCCGATGCGCTGAGTCGGAAGGGCGAACATGCGGCCCTGTGCATGTTTGCCCATATTCATTCAAGTAAGATCGATGGATCGATGCGCGACATCATGAAGGAACATTTACACAAAGACCCATCGGCAAAGCTGTTGTCGAACTAG

mRNA sequence

ATGTTCGTAGATGCCACAATAAACTCTCAACCGAGCAAGAGCACTCTGATAGACTCTGGAGCAACCCACAACTTCATTGCCGATCAAGAAGCCAGAAGATTGGGACTCACCATAGGAAAAGACCCGGGGAAAATGAAAGCTGTCAACTCCGAGGCCTTGCCTATTGTGGGAGTTTCCAAGAGAGTCCCCTTCAAAATAGGGGATTGGACTGGAGAGCTAGACCTTGTCGTGGTTCGCATGGACGACTTCGACGTGGTACTTGGGATGGAGTTCCTCCTAGAACACAAGGTCATCCCAATGCCATTGGCAAAATGCTTGGTGATCACCGACCGTAACCCCACAGTAATACATGCAAGCATCAAGCAACCAGGTAATCTTCGAATGATCTCGGCCATACAACTGAAAAGGGGACTCGCACGAGAGAAACCTACATTCATGGCCATACCACTGATAGAGGAAGCAACCACCGAAGAGACTGTCCCAGAGGAAATCAAGGAAGTACTAGACAGCTATACCGACATAATGCCAGAGAGTCTACCACAAACACTACCACCTCGTCGAGGCATTGACCACGAAATCAAACTCCTTCCCGGGGTTAAGCCGCCAGCAAAGAACGCATACCGGATGGCTCCGCCCGAGCTAGCTGAATTGAGGAAACAACTGGATGAGTTGTTGAAGGCGGGATTCATTCGCCCGGCAAAGGCCCCTTATGGAGCCCTCATACTGTTCCAGAAGAAGAAGGATGGGACGTTGCGTCTATGCATAGACTATAGAGCCTTAAACAAGGTGACAGTACGCAACAAATATCCTTTGCCGATAATATCCGACTTGTTCGACCAACTTCACGGGGCCAAGTACTTCACGAAGTTGGACTTACGATCAGGATATTATCAAGTACGTATTGCCGAAGGGGACGAACCTAAGACGACGTGCGTAACAAGATATGGGGCCTTTGAATTCCTAGTAATGCCCTTTGGTTTGACAAACGCCCCAGCTACGTTCTGCACGTTGATGAACCAGGTTTTCTATGAATACCTGGATCAGTTCGTCATAGTATACCTCGACGACATTGTTGTTTACAGCACAACCCTCGAGGAACACAAAGTGCATTTGAAGTTAGTGTTTGACAAGCTCCGGCAGAATCAGCTGTATGTGAAGAAGGAGAAATGTGCATTCGCACAAACATGCATCAACTTCCTTGGACATGTCGTCAGATGTGGACAAATTAGTATGGATAGCGACAAGATAAAAGCTATCCAAGAATGGAAGGTTCCTACTTCCGTATCTGAGTTGCGGTCCTTCTTAGGACTAGCCAACTACTATAGGCGGTTCGTCGAAGGGTTTTCACGACGAGCCGCCCCATTGACAGAGCTGTTGAAGAAAGACCACCCTTGGTCGTGGTCGAATGATTGTCAAATGGCCTTTGAAAATCTGAAAACAACCATGACGAGGGGTCCTGTCCTCGGGTTGGTAGACGTCACAAAGCCATTTGAAGTAGAAACCGATGCTTCGGACTTTGCTCTCGGTGGAGTCCTTATTCAAGAAGGCCACCCCATCACTTACGAAAGTCGAAAGCTCAACGATGCCGAACGAAGATACACTGTCTCCGAGAAAGAAATGCAGGCTGTAGTCCATTGCCTTCGAGTCTGGAGACAGTATCTCTTAGGATCACAGTTCGTAGTGAAGACGGATAACAGCGCCACTTGCTACTTCTTTGATCAACCAAAATTAATAGCAAAACAAGCCCGGTGGCAGGAGTCGTTGGCCGAATTCGACTTCAAGTTCGAACACAAAGCAGGAAAGAGCAATCAAGCAGCCGATGCGCTGAGTCGGAAGGGCGAACATGCGGCCCTGTGCATGTTTGCCCATATTCATTCAAGTAAGATCGATGGATCGATGCGCGACATCATGAAGGAACATTTACACAAAGACCCATCGGCAAAGCTGTTGTCGAACTAG

Coding sequence (CDS)

ATGTTCGTAGATGCCACAATAAACTCTCAACCGAGCAAGAGCACTCTGATAGACTCTGGAGCAACCCACAACTTCATTGCCGATCAAGAAGCCAGAAGATTGGGACTCACCATAGGAAAAGACCCGGGGAAAATGAAAGCTGTCAACTCCGAGGCCTTGCCTATTGTGGGAGTTTCCAAGAGAGTCCCCTTCAAAATAGGGGATTGGACTGGAGAGCTAGACCTTGTCGTGGTTCGCATGGACGACTTCGACGTGGTACTTGGGATGGAGTTCCTCCTAGAACACAAGGTCATCCCAATGCCATTGGCAAAATGCTTGGTGATCACCGACCGTAACCCCACAGTAATACATGCAAGCATCAAGCAACCAGGTAATCTTCGAATGATCTCGGCCATACAACTGAAAAGGGGACTCGCACGAGAGAAACCTACATTCATGGCCATACCACTGATAGAGGAAGCAACCACCGAAGAGACTGTCCCAGAGGAAATCAAGGAAGTACTAGACAGCTATACCGACATAATGCCAGAGAGTCTACCACAAACACTACCACCTCGTCGAGGCATTGACCACGAAATCAAACTCCTTCCCGGGGTTAAGCCGCCAGCAAAGAACGCATACCGGATGGCTCCGCCCGAGCTAGCTGAATTGAGGAAACAACTGGATGAGTTGTTGAAGGCGGGATTCATTCGCCCGGCAAAGGCCCCTTATGGAGCCCTCATACTGTTCCAGAAGAAGAAGGATGGGACGTTGCGTCTATGCATAGACTATAGAGCCTTAAACAAGGTGACAGTACGCAACAAATATCCTTTGCCGATAATATCCGACTTGTTCGACCAACTTCACGGGGCCAAGTACTTCACGAAGTTGGACTTACGATCAGGATATTATCAAGTACGTATTGCCGAAGGGGACGAACCTAAGACGACGTGCGTAACAAGATATGGGGCCTTTGAATTCCTAGTAATGCCCTTTGGTTTGACAAACGCCCCAGCTACGTTCTGCACGTTGATGAACCAGGTTTTCTATGAATACCTGGATCAGTTCGTCATAGTATACCTCGACGACATTGTTGTTTACAGCACAACCCTCGAGGAACACAAAGTGCATTTGAAGTTAGTGTTTGACAAGCTCCGGCAGAATCAGCTGTATGTGAAGAAGGAGAAATGTGCATTCGCACAAACATGCATCAACTTCCTTGGACATGTCGTCAGATGTGGACAAATTAGTATGGATAGCGACAAGATAAAAGCTATCCAAGAATGGAAGGTTCCTACTTCCGTATCTGAGTTGCGGTCCTTCTTAGGACTAGCCAACTACTATAGGCGGTTCGTCGAAGGGTTTTCACGACGAGCCGCCCCATTGACAGAGCTGTTGAAGAAAGACCACCCTTGGTCGTGGTCGAATGATTGTCAAATGGCCTTTGAAAATCTGAAAACAACCATGACGAGGGGTCCTGTCCTCGGGTTGGTAGACGTCACAAAGCCATTTGAAGTAGAAACCGATGCTTCGGACTTTGCTCTCGGTGGAGTCCTTATTCAAGAAGGCCACCCCATCACTTACGAAAGTCGAAAGCTCAACGATGCCGAACGAAGATACACTGTCTCCGAGAAAGAAATGCAGGCTGTAGTCCATTGCCTTCGAGTCTGGAGACAGTATCTCTTAGGATCACAGTTCGTAGTGAAGACGGATAACAGCGCCACTTGCTACTTCTTTGATCAACCAAAATTAATAGCAAAACAAGCCCGGTGGCAGGAGTCGTTGGCCGAATTCGACTTCAAGTTCGAACACAAAGCAGGAAAGAGCAATCAAGCAGCCGATGCGCTGAGTCGGAAGGGCGAACATGCGGCCCTGTGCATGTTTGCCCATATTCATTCAAGTAAGATCGATGGATCGATGCGCGACATCATGAAGGAACATTTACACAAAGACCCATCGGCAAAGCTGTTGTCGAACTAG

Protein sequence

MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSKRVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASIKQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLPQTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGALILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVYSTTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQEWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKTTMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEMQAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAGKSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLLSN
Homology
BLAST of CmoCh13G003730 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.3e-85
Identity = 163/402 (40.55%), Postives = 250/402 (62.19%), Query Frame = 0

Query: 216 ELRKQLDELLKAGFIRPAKAPYGALI-LFQKKKDGT----LRLCIDYRALNKVTVRNKYP 275
           E+  Q+ ++L  G IR + +PY + I +  KK+D +     R+ IDYR LN++TV +++P
Sbjct: 222 EVESQIQDMLNQGIIRTSNSPYNSPIWVVPKKQDASGKQKFRIVIDYRKLNEITVGDRHP 281

Query: 276 LPIISDLFDQLHGAKYFTKLDLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNA 335
           +P + ++  +L    YFT +DL  G++Q+ +      KT   T++G +E+L MPFGL NA
Sbjct: 282 IPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGLKNA 341

Query: 336 PATFCTLMNQVFYEYLDQFVIVYLDDIVVYSTTLEEHKVHLKLVFDKLRQNQLYVKKEKC 395
           PATF   MN +    L++  +VYLDDI+V+ST+L+EH   L LVF+KL +  L ++ +KC
Sbjct: 342 PATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKC 401

Query: 396 AFAQTCINFLGHVVRCGQISMDSDKIKAIQEWKVPTSVSELRSFLGLANYYRRFVEGFSR 455
            F +    FLGHV+    I  + +KI+AIQ++ +PT   E+++FLGL  YYR+F+  F+ 
Sbjct: 402 EFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFAD 461

Query: 456 RAAPLTELLKKDHPWSWSN-DCQMAFENLKTTMTRGPVLGLVDVTKPFEVETDASDFALG 515
            A P+T+ LKK+     +N +   AF+ LK  ++  P+L + D TK F + TDASD ALG
Sbjct: 462 IAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALG 521

Query: 516 GVLIQEGHPITYESRKLNDAERRYTVSEKEMQAVVHCLRVWRQYLLGSQFVVKTDNSATC 575
            VL Q+GHP++Y SR LN+ E  Y+  EKE+ A+V   + +R YLLG  F + +D+    
Sbjct: 522 AVLSQDGHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLS 581

Query: 576 YFFDQPKLIAKQARWQESLAEFDFKFEHKAGKSNQAADALSR 612
           + +      +K  RW+  L+EFDF  ++  GK N  ADALSR
Sbjct: 582 WLYRMKDPNSKLTRWRVKLSEFDFDIKYIKGKENCVADALSR 623

BLAST of CmoCh13G003730 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.4e-84
Identity = 167/417 (40.05%), Postives = 246/417 (58.99%), Query Frame = 0

Query: 201 PPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGA-LILFQKKKDGT----LRLCI 260
           P     Y +A     E+  Q+ E+L  G IR + +PY +   +  KK D +     R+ I
Sbjct: 206 PIYSKQYPLAQTHEIEVENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVVI 265

Query: 261 DYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVRIAEGDEPKTTCVTRY 320
           DYR LN++T+ ++YP+P + ++  +L   +YFT +DL  G++Q+ + E    KT   T+ 
Sbjct: 266 DYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTKS 325

Query: 321 GAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVYSTTLEEHKVHLKLVF 380
           G +E+L MPFGL NAPATF   MN +    L++  +VYLDDI+++ST+L EH   ++LVF
Sbjct: 326 GHYEYLRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVF 385

Query: 381 DKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQEWKVPTSVSELRSFL 440
            KL    L ++ +KC F +   NFLGH+V    I  +  K+KAI  + +PT   E+R+FL
Sbjct: 386 TKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAFL 445

Query: 441 GLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSN-DCQMAFENLKTTMTRGPVLGLVDVT 500
           GL  YYR+F+  ++  A P+T  LKK         +   AFE LK  + R P+L L D  
Sbjct: 446 GLTGYYRKFIPNYADIAKPMTSCLKKRTKIDTQKLEYIEAFEKLKALIIRDPILQLPDFE 505

Query: 501 KPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEMQAVVHCLRVWRQYL 560
           K F + TDAS+ ALG VL Q GHPI++ SR LND E  Y+  EKE+ A+V   + +R YL
Sbjct: 506 KKFVLTTDASNLALGAVLSQNGHPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYL 565

Query: 561 LGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAGKSNQAADALSR 612
           LG QF++ +D+    +  +  +  AK  RW+  L+E+ FK ++  GK N  ADALSR
Sbjct: 566 LGRQFLIASDHQPLRWLHNLKEPGAKLERWRVRLSEYQFKIDYIKGKENSVADALSR 622

BLAST of CmoCh13G003730 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.1e-81
Identity = 172/484 (35.54%), Postives = 269/484 (55.58%), Query Frame = 0

Query: 138 LAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLPQTLPPRRGIDHEIKLL- 197
           ++  K T   +  +     E  +P+  KE  D   +   E LP+   P +G++ E++L  
Sbjct: 352 ISSSKHTLSQMNKVSNIVKEPELPDIYKEFKDITAETNTEKLPK---PIKGLEFEVELTQ 411

Query: 198 PGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGALILFQKKKDGTLRLCID 257
              + P +N Y + P ++  +  ++++ LK+G IR +KA     ++F  KK+GTLR+ +D
Sbjct: 412 ENYRLPIRN-YPLPPGKMQAMNDEINQGLKSGIIRESKAINACPVMFVPKKEGTLRMVVD 471

Query: 258 YRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVRIAEGDEPKTTCVTRYG 317
           Y+ LNK    N YPLP+I  L  ++ G+  FTKLDL+S Y+ +R+ +GDE K       G
Sbjct: 472 YKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRKGDEHKLAFRCPRG 531

Query: 318 AFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVYSTTLEEHKVHLKLVFD 377
            FE+LVMP+G++ APA F   +N +  E  +  V+ Y+DDI+++S +  EH  H+K V  
Sbjct: 532 VFEYLVMPYGISTAPAHFQYFINTILGEAKESHVVCYMDDILIHSKSESEHVKHVKDVLQ 591

Query: 378 KLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQEWKVPTSVSELRSFLG 437
           KL+   L + + KC F Q+ + F+G+ +     +   + I  + +WK P +  ELR FLG
Sbjct: 592 KLKNANLIINQAKCEFHQSQVKFIGYHISEKGFTPCQENIDKVLQWKQPKNRKELRQFLG 651

Query: 438 LANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKTTMTRGPVLGLVDVTKP 497
             NY R+F+   S+   PL  LLKKD  W W+     A EN+K  +   PVL   D +K 
Sbjct: 652 SVNYLRKFIPKTSQLTHPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKK 711

Query: 498 FEVETDASDFALGGVLIQEG-----HPITYESRKLNDAERRYTVSEKEMQAVVHCLRVWR 557
             +ETDASD A+G VL Q+      +P+ Y S K++ A+  Y+VS+KEM A++  L+ WR
Sbjct: 712 ILLETDASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWR 771

Query: 558 QYLLGS--QFVVKTD--NSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAGKSNQAAD 612
            YL  +   F + TD  N       +      + ARWQ  L +F+F+  ++ G +N  AD
Sbjct: 772 HYLESTIEPFKILTDHRNLIGRITNESEPENKRLARWQLFLQDFNFEINYRPGSANHIAD 831

BLAST of CmoCh13G003730 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.1e-81
Identity = 172/484 (35.54%), Postives = 269/484 (55.58%), Query Frame = 0

Query: 138 LAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLPQTLPPRRGIDHEIKLL- 197
           ++  K T   +  +     E  +P+  KE  D   +   E LP+   P +G++ E++L  
Sbjct: 352 ISSSKHTLSQMNKVSNIVKEPELPDIYKEFKDITAETNTEKLPK---PIKGLEFEVELTQ 411

Query: 198 PGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGALILFQKKKDGTLRLCID 257
              + P +N Y + P ++  +  ++++ LK+G IR +KA     ++F  KK+GTLR+ +D
Sbjct: 412 ENYRLPIRN-YPLPPGKMQAMNDEINQGLKSGIIRESKAINACPVMFVPKKEGTLRMVVD 471

Query: 258 YRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVRIAEGDEPKTTCVTRYG 317
           Y+ LNK    N YPLP+I  L  ++ G+  FTKLDL+S Y+ +R+ +GDE K       G
Sbjct: 472 YKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRKGDEHKLAFRCPRG 531

Query: 318 AFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVYSTTLEEHKVHLKLVFD 377
            FE+LVMP+G++ APA F   +N +  E  +  V+ Y+DDI+++S +  EH  H+K V  
Sbjct: 532 VFEYLVMPYGISTAPAHFQYFINTILGEAKESHVVCYMDDILIHSKSESEHVKHVKDVLQ 591

Query: 378 KLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQEWKVPTSVSELRSFLG 437
           KL+   L + + KC F Q+ + F+G+ +     +   + I  + +WK P +  ELR FLG
Sbjct: 592 KLKNANLIINQAKCEFHQSQVKFIGYHISEKGFTPCQENIDKVLQWKQPKNRKELRQFLG 651

Query: 438 LANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKTTMTRGPVLGLVDVTKP 497
             NY R+F+   S+   PL  LLKKD  W W+     A EN+K  +   PVL   D +K 
Sbjct: 652 SVNYLRKFIPKTSQLTHPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKK 711

Query: 498 FEVETDASDFALGGVLIQEG-----HPITYESRKLNDAERRYTVSEKEMQAVVHCLRVWR 557
             +ETDASD A+G VL Q+      +P+ Y S K++ A+  Y+VS+KEM A++  L+ WR
Sbjct: 712 ILLETDASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWR 771

Query: 558 QYLLGS--QFVVKTD--NSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAGKSNQAAD 612
            YL  +   F + TD  N       +      + ARWQ  L +F+F+  ++ G +N  AD
Sbjct: 772 HYLESTIEPFKILTDHRNLIGRITNESEPENKRLARWQLFLQDFNFEINYRPGSANHIAD 831

BLAST of CmoCh13G003730 vs. ExPASy Swiss-Prot
Match: P0CT35 (Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-2 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.1e-81
Identity = 172/484 (35.54%), Postives = 269/484 (55.58%), Query Frame = 0

Query: 138 LAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLPQTLPPRRGIDHEIKLL- 197
           ++  K T   +  +     E  +P+  KE  D   +   E LP+   P +G++ E++L  
Sbjct: 352 ISSSKHTLSQMNKVSNIVKEPELPDIYKEFKDITAETNTEKLPK---PIKGLEFEVELTQ 411

Query: 198 PGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGALILFQKKKDGTLRLCID 257
              + P +N Y + P ++  +  ++++ LK+G IR +KA     ++F  KK+GTLR+ +D
Sbjct: 412 ENYRLPIRN-YPLPPGKMQAMNDEINQGLKSGIIRESKAINACPVMFVPKKEGTLRMVVD 471

Query: 258 YRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVRIAEGDEPKTTCVTRYG 317
           Y+ LNK    N YPLP+I  L  ++ G+  FTKLDL+S Y+ +R+ +GDE K       G
Sbjct: 472 YKPLNKYVKPNIYPLPLIEQLLAKIQGSTIFTKLDLKSAYHLIRVRKGDEHKLAFRCPRG 531

Query: 318 AFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVYSTTLEEHKVHLKLVFD 377
            FE+LVMP+G++ APA F   +N +  E  +  V+ Y+DDI+++S +  EH  H+K V  
Sbjct: 532 VFEYLVMPYGISTAPAHFQYFINTILGEAKESHVVCYMDDILIHSKSESEHVKHVKDVLQ 591

Query: 378 KLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQEWKVPTSVSELRSFLG 437
           KL+   L + + KC F Q+ + F+G+ +     +   + I  + +WK P +  ELR FLG
Sbjct: 592 KLKNANLIINQAKCEFHQSQVKFIGYHISEKGFTPCQENIDKVLQWKQPKNRKELRQFLG 651

Query: 438 LANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKTTMTRGPVLGLVDVTKP 497
             NY R+F+   S+   PL  LLKKD  W W+     A EN+K  +   PVL   D +K 
Sbjct: 652 SVNYLRKFIPKTSQLTHPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKK 711

Query: 498 FEVETDASDFALGGVLIQEG-----HPITYESRKLNDAERRYTVSEKEMQAVVHCLRVWR 557
             +ETDASD A+G VL Q+      +P+ Y S K++ A+  Y+VS+KEM A++  L+ WR
Sbjct: 712 ILLETDASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWR 771

Query: 558 QYLLGS--QFVVKTD--NSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAGKSNQAAD 612
            YL  +   F + TD  N       +      + ARWQ  L +F+F+  ++ G +N  AD
Sbjct: 772 HYLESTIEPFKILTDHRNLIGRITNESEPENKRLARWQLFLQDFNFEINYRPGSANHIAD 831

BLAST of CmoCh13G003730 vs. ExPASy TrEMBL
Match: A0A6J1D906 (Reverse transcriptase OS=Momordica charantia OX=3673 GN=LOC111018360 PE=4 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 522/648 (80.56%), Postives = 580/648 (89.51%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            MFVDATIN   +KST++DSGATHNFI++QEARRL LTI KD GKMK VN EALPIVGVSK
Sbjct: 455  MFVDATINCNXAKSTMVDSGATHNFISEQEARRLKLTIEKDTGKMKVVNFEALPIVGVSK 514

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            RV  K+G WTG +D VVVRMDDFDVVLGMEFL+EHKVIPMPLAKC+++T  +PTV+  SI
Sbjct: 515  RVMLKLGTWTGSVDFVVVRMDDFDVVLGMEFLIEHKVIPMPLAKCMIVTSNSPTVVTTSI 574

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            KQPG +RMISA+QLK+GL RE+PTFMAIP++E+      VP EI+ V+  Y DIMP+SLP
Sbjct: 575  KQPGGIRMISALQLKKGLNREEPTFMAIPMVEQPVETRDVPPEIQVVMKEYVDIMPDSLP 634

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            +TLPPRRGIDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAP+GA 
Sbjct: 635  KTLPPRRGIDHEIELIPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPFGAP 694

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQKKKDGTLRLCIDYRALNKVTVRNKYPLPII+DLFDQLHGAKYFTKLDLRSGYYQVR
Sbjct: 695  VLFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIITDLFDQLHGAKYFTKLDLRSGYYQVR 754

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVM FGLTNAPATFCT+MNQVF+EYLDQFV+VYLDDIVVY
Sbjct: 755  IAEGDEPKTTCVTRYGAFEFLVMXFGLTNAPATFCTMMNQVFHEYLDQFVVVYLDDIVVY 814

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            S TL+EH++HL+LVFDKLRQNQLYVKKEKCAFAQ  I FLGHV+  GQISMD+DK+KAIQ
Sbjct: 815  SPTLKEHQIHLRLVFDKLRQNQLYVKKEKCAFAQKRIXFLGHVIEQGQISMDTDKVKAIQ 874

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            EW+VPTSV ELRSFLGLANYYRRF+EGFSRRA P+TELLKK   W WS + Q AFE+LK 
Sbjct: 875  EWRVPTSVIELRSFLGLANYYRRFIEGFSRRATPMTELLKKGMTWMWSKESQDAFEDLKA 934

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             M +GPVLGL DVTKPFEVETDASD+ALGGVL+Q+ HPI YESRKLN+AERRYTVSEKEM
Sbjct: 935  AMMKGPVLGLADVTKPFEVETDASDYALGGVLLQDDHPIXYESRKLNNAERRYTVSEKEM 994

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSA C+FF+QPKL +KQARWQE LAEFDFKFEHKAG
Sbjct: 995  LAVVHCLRSWRQYLLGSXFVVKTDNSAICHFFNQPKLTSKQARWQELLAEFDFKFEHKAG 1054

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAK 649
            KSNQAADALSRKGEHA LCM AHIH+SK DGS+RD++ E+L   PSA+
Sbjct: 1055 KSNQAADALSRKGEHAXLCMLAHIHTSKADGSIRDLIXEYLQXXPSAR 1102

BLAST of CmoCh13G003730 vs. ExPASy TrEMBL
Match: A0A5D3C9P8 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00750 PE=4 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 8.3e-293
Identity = 487/652 (74.69%), Postives = 567/652 (86.96%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            M+VD  IN +P+KST++DSGATHNFI + EA+RL L   KD G+MKAVNS ALPI+G+ K
Sbjct: 436  MYVDTWINEKPTKSTMVDSGATHNFITEAEAKRLNLRWEKDAGRMKAVNSAALPIIGLVK 495

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            R   ++G W+G +D VVV+MDDFDVVLGMEFLLEH+VIPMPLAKCLVIT   P+V+   +
Sbjct: 496  RTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDL 555

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            +QP  L+MISA+QLK+GL+R++PTFMAIPL     + ETVP+EI  VL+ Y D+MP+SLP
Sbjct: 556  RQPDGLKMISAMQLKKGLSRDEPTFMAIPLNSSENSGETVPKEIVRVLEKYRDVMPDSLP 615

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            ++LPPRR IDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAPYGA 
Sbjct: 616  KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 675

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQKKKDG+LRLCIDYRALNK+TVRNKYPLPII+DLFD+LHGAKYF+KLDLRSGYYQVR
Sbjct: 676  VLFQKKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVR 735

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVF+EYLD+FV+VYLDDIVVY
Sbjct: 736  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVY 795

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STT+EEH+ HL+ VF KL++NQLYVK+EKC+FAQ  INFLGHV+ CG+I M+  KI AI+
Sbjct: 796  STTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKIAAIR 855

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            +W +P SVSELRSFLGLANYYRRFVEGFS+RA+PLTELLKKD  W+W  +CQ AF+ LK 
Sbjct: 856  DWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLTELLKKDVHWNWDPECQAAFDGLKQ 915

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             +  GP+LG+ DVTKPFEVETDASD+ALGGVL+Q GHPI YESRKLN AERRYTVSEKEM
Sbjct: 916  ALMEGPLLGIADVTKPFEVETDASDYALGGVLLQNGHPIAYESRKLNAAERRYTVSEKEM 975

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSATC+FF QPKL +KQARWQE LAEFDF+FEHK G
Sbjct: 976  LAVVHCLRAWRQYLLGSSFVVKTDNSATCHFFTQPKLTSKQARWQEFLAEFDFEFEHKKG 1035

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLLSN 653
             SNQAADALSRK EHAA+C+ AH+  S+I GS+RD ++E L KD +A+ + N
Sbjct: 1036 SSNQAADALSRKQEHAAICLLAHLQGSEIGGSVRDTLREFLQKDHAAQNVMN 1087

BLAST of CmoCh13G003730 vs. ExPASy TrEMBL
Match: A0A5D3C4R1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001990 PE=4 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.8e-292
Identity = 486/652 (74.54%), Postives = 567/652 (86.96%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            M+VD  IN +P+KST++DSGATHNFI + EA+RL L   KD G+MKAVNS ALPI+G+ K
Sbjct: 466  MYVDTWINQKPTKSTMVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVK 525

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            R   ++G W+G +D VVV+MDDFDVVLGMEFLLEH+VIPMPLAKCLVIT   P+V+   +
Sbjct: 526  RTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDL 585

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            +QP  L+MISA+QLK+GL+R++PTFMAIPL     + ETVP+EI  VL+ Y D+MP+SLP
Sbjct: 586  RQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLP 645

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            ++LPPRR IDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAPYGA 
Sbjct: 646  KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 705

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQ+KKDG+LRLCIDYRALNK+TVRNKYPLPII+DLFD+LHGAKYF+KLDLRSGYYQVR
Sbjct: 706  VLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVR 765

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVF+EYLD+FV+VYLDDIVVY
Sbjct: 766  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVY 825

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STT+EEH+ HL+ VF KL++NQLYVK+EKC+FAQ  INFLGHV+ CG+I M+  KI AI+
Sbjct: 826  STTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKIAAIR 885

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            +W +P SVSELRSFLGLANYYRRFVEGFS+RA+PLTELLKKD  W+W  +CQ AF+ LK 
Sbjct: 886  DWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLTELLKKDVHWNWDPECQTAFDGLKQ 945

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             +  GP+LG+ DVTKPFEVETDASD+ALGGVL+Q GHPI YESRKLN AERRYTVSEKEM
Sbjct: 946  ALMEGPLLGIADVTKPFEVETDASDYALGGVLLQNGHPIAYESRKLNAAERRYTVSEKEM 1005

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSATC+FF QPKL +KQARWQE LAEFDF+FEHK G
Sbjct: 1006 LAVVHCLRAWRQYLLGSSFVVKTDNSATCHFFTQPKLTSKQARWQEFLAEFDFEFEHKKG 1065

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLLSN 653
             SNQAADALSRK EHAA+C+ AH+  S+I GS+RD ++E L KD +A+ + N
Sbjct: 1066 SSNQAADALSRKQEHAAICLLAHLQGSEIGGSVRDTLREFLQKDHAAQNVMN 1117

BLAST of CmoCh13G003730 vs. ExPASy TrEMBL
Match: A0A5D3BRZ6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00060 PE=4 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.8e-292
Identity = 486/652 (74.54%), Postives = 567/652 (86.96%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            M+VD  IN +P+KST++DSGATHNFI + EA+RL L   KD G+MKAVNS ALPI+G+ K
Sbjct: 466  MYVDTWINQKPTKSTMVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVK 525

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            R   ++G W+G +D VVV+MDDFDVVLGMEFLLEH+VIPMPLAKCLVIT   P+V+   +
Sbjct: 526  RTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDL 585

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            +QP  L+MISA+QLK+GL+R++PTFMAIPL     + ETVP+EI  VL+ Y D+MP+SLP
Sbjct: 586  RQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLP 645

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            ++LPPRR IDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAPYGA 
Sbjct: 646  KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 705

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQ+KKDG+LRLCIDYRALNK+TVRNKYPLPII+DLFD+LHGAKYF+KLDLRSGYYQVR
Sbjct: 706  VLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVR 765

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVF+EYLD+FV+VYLDDIVVY
Sbjct: 766  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVY 825

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STT+EEH+ HL+ VF KL++NQLYVK+EKC+FAQ  INFLGHV+ CG+I M+  KI AI+
Sbjct: 826  STTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKIAAIR 885

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            +W +P SVSELRSFLGLANYYRRFVEGFS+RA+PLTELLKKD  W+W  +CQ AF+ LK 
Sbjct: 886  DWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLTELLKKDVHWNWDPECQTAFDGLKQ 945

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             +  GP+LG+ DVTKPFEVETDASD+ALGGVL+Q GHPI YESRKLN AERRYTVSEKEM
Sbjct: 946  ALMEGPLLGIADVTKPFEVETDASDYALGGVLLQNGHPIAYESRKLNAAERRYTVSEKEM 1005

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSATC+FF QPKL +KQARWQE LAEFDF+FEHK G
Sbjct: 1006 LAVVHCLRAWRQYLLGSSFVVKTDNSATCHFFTQPKLTSKQARWQEFLAEFDFEFEHKKG 1065

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLLSN 653
             SNQAADALSRK EHAA+C+ AH+  S+I GS+RD ++E L KD +A+ + N
Sbjct: 1066 SSNQAADALSRKQEHAAICLLAHLQGSEIGGSVRDTLREFLQKDHAAQNVMN 1117

BLAST of CmoCh13G003730 vs. ExPASy TrEMBL
Match: A0A5A7T0E2 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20G001310 PE=4 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.8e-292
Identity = 486/652 (74.54%), Postives = 567/652 (86.96%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            M+VD  IN +P+KST++DSGATHNFI + EA+RL L   KD G+MKAVNS ALPI+G+ K
Sbjct: 466  MYVDTWINQKPTKSTMVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVK 525

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            R   ++G W+G +D VVV+MDDFDVVLGMEFLLEH+VIPMPLAKCLVIT   P+V+   +
Sbjct: 526  RTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDL 585

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            +QP  L+MISA+QLK+GL+R++PTFMAIPL     + ETVP+EI  VL+ Y D+MP+SLP
Sbjct: 586  RQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLP 645

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            ++LPPRR IDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAPYGA 
Sbjct: 646  KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 705

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQ+KKDG+LRLCIDYRALNK+TVRNKYPLPII+DLFD+LHGAKYF+KLDLRSGYYQVR
Sbjct: 706  VLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVR 765

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVF+EYLD+FV+VYLDDIVVY
Sbjct: 766  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVY 825

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STT+EEH+ HL+ VF KL++NQLYVK+EKC+FAQ  INFLGHV+ CG+I M+  KI AI+
Sbjct: 826  STTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKIAAIR 885

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            +W +P SVSELRSFLGLANYYRRFVEGFS+RA+PLTELLKKD  W+W  +CQ AF+ LK 
Sbjct: 886  DWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLTELLKKDVHWNWDPECQTAFDGLKQ 945

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             +  GP+LG+ DVTKPFEVETDASD+ALGGVL+Q GHPI YESRKLN AERRYTVSEKEM
Sbjct: 946  ALMEGPLLGIADVTKPFEVETDASDYALGGVLLQNGHPIAYESRKLNAAERRYTVSEKEM 1005

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSATC+FF QPKL +KQARWQE LAEFDF+FEHK G
Sbjct: 1006 LAVVHCLRAWRQYLLGSSFVVKTDNSATCHFFTQPKLTSKQARWQEFLAEFDFEFEHKKG 1065

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLLSN 653
             SNQAADALSRK EHAA+C+ AH+  S+I GS+RD ++E L KD +A+ + N
Sbjct: 1066 SSNQAADALSRKQEHAAICLLAHLQGSEIGGSIRDTLREFLQKDHAAQNVMN 1117

BLAST of CmoCh13G003730 vs. NCBI nr
Match: XP_023537907.1 (uncharacterized protein LOC111798805 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1271.9 bits (3290), Expect = 0.0e+00
Identity = 632/648 (97.53%), Postives = 640/648 (98.77%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            MFVDATINS+ SKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK
Sbjct: 461  MFVDATINSRLSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 520

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
             VPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVI ASI
Sbjct: 521  GVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIPASI 580

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            KQPGNLRMISAIQLKRGLARE+PTFMAIPLIEEATTEETVPEEIK+VLDSYTDIMPESLP
Sbjct: 581  KQPGNLRMISAIQLKRGLAREEPTFMAIPLIEEATTEETVPEEIKDVLDSYTDIMPESLP 640

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            QTLPPRRGIDHEI+LLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGA 
Sbjct: 641  QTLPPRRGIDHEIELLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAP 700

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR
Sbjct: 701  VLFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 760

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY
Sbjct: 761  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 820

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCI+FLGHVVRCGQISMDSDKIKAIQ
Sbjct: 821  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCIHFLGHVVRCGQISMDSDKIKAIQ 880

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT
Sbjct: 881  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 940

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
            TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPI YESRKLNDAERRYTVSEKEM
Sbjct: 941  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPIAYESRKLNDAERRYTVSEKEM 1000

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLRVWRQYLLGSQFVVKTDNSATC+FFDQPKL AKQARWQESLAEFDFKFEHKAG
Sbjct: 1001 LAVVHCLRVWRQYLLGSQFVVKTDNSATCHFFDQPKLTAKQARWQESLAEFDFKFEHKAG 1060

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAK 649
            KSNQAADALSRKGEHAALCM AHIHSSKIDGSMRDI+KEHLHKDPSAK
Sbjct: 1061 KSNQAADALSRKGEHAALCMLAHIHSSKIDGSMRDIIKEHLHKDPSAK 1108

BLAST of CmoCh13G003730 vs. NCBI nr
Match: XP_023524533.1 (uncharacterized protein LOC111788429 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 631/650 (97.08%), Postives = 640/650 (98.46%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            MFVDATINS+ SKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK
Sbjct: 461  MFVDATINSRLSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 520

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
             VPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVI ASI
Sbjct: 521  GVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIPASI 580

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            KQPGNLRMISAIQLKRGLARE+PTFMAIPLIEEATTEETVPEEIKEVLD+YTDIMPESLP
Sbjct: 581  KQPGNLRMISAIQLKRGLAREEPTFMAIPLIEEATTEETVPEEIKEVLDNYTDIMPESLP 640

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            QTLPPRRGIDHEI+LLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGA 
Sbjct: 641  QTLPPRRGIDHEIELLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAP 700

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR
Sbjct: 701  VLFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 760

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            I EGDEPKTTCVTRYGAFEFLVMPFGLTNAPATF TLMNQVFYEYLDQFVIVYLDDIVVY
Sbjct: 761  IVEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFYTLMNQVFYEYLDQFVIVYLDDIVVY 820

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ
Sbjct: 821  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 880

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT
Sbjct: 881  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 940

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
            TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPI YESRKLNDAERRYTVSEKEM
Sbjct: 941  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPIAYESRKLNDAERRYTVSEKEM 1000

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLRVWRQYLLGSQFVVKTDNSATC+FFDQPKL AKQARWQESLAEFDFKFEHKAG
Sbjct: 1001 LAVVHCLRVWRQYLLGSQFVVKTDNSATCHFFDQPKLTAKQARWQESLAEFDFKFEHKAG 1060

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLL 651
            KSNQAADALSRKGEHAALCM AHIHSSKIDGSMRDI+KEHLHKDPSAK++
Sbjct: 1061 KSNQAADALSRKGEHAALCMLAHIHSSKIDGSMRDIIKEHLHKDPSAKVV 1110

BLAST of CmoCh13G003730 vs. NCBI nr
Match: XP_022150099.1 (uncharacterized protein LOC111018360 [Momordica charantia])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 522/648 (80.56%), Postives = 580/648 (89.51%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            MFVDATIN   +KST++DSGATHNFI++QEARRL LTI KD GKMK VN EALPIVGVSK
Sbjct: 455  MFVDATINCNXAKSTMVDSGATHNFISEQEARRLKLTIEKDTGKMKVVNFEALPIVGVSK 514

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            RV  K+G WTG +D VVVRMDDFDVVLGMEFL+EHKVIPMPLAKC+++T  +PTV+  SI
Sbjct: 515  RVMLKLGTWTGSVDFVVVRMDDFDVVLGMEFLIEHKVIPMPLAKCMIVTSNSPTVVTTSI 574

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            KQPG +RMISA+QLK+GL RE+PTFMAIP++E+      VP EI+ V+  Y DIMP+SLP
Sbjct: 575  KQPGGIRMISALQLKKGLNREEPTFMAIPMVEQPVETRDVPPEIQVVMKEYVDIMPDSLP 634

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            +TLPPRRGIDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAP+GA 
Sbjct: 635  KTLPPRRGIDHEIELIPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPFGAP 694

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQKKKDGTLRLCIDYRALNKVTVRNKYPLPII+DLFDQLHGAKYFTKLDLRSGYYQVR
Sbjct: 695  VLFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIITDLFDQLHGAKYFTKLDLRSGYYQVR 754

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVM FGLTNAPATFCT+MNQVF+EYLDQFV+VYLDDIVVY
Sbjct: 755  IAEGDEPKTTCVTRYGAFEFLVMXFGLTNAPATFCTMMNQVFHEYLDQFVVVYLDDIVVY 814

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            S TL+EH++HL+LVFDKLRQNQLYVKKEKCAFAQ  I FLGHV+  GQISMD+DK+KAIQ
Sbjct: 815  SPTLKEHQIHLRLVFDKLRQNQLYVKKEKCAFAQKRIXFLGHVIEQGQISMDTDKVKAIQ 874

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            EW+VPTSV ELRSFLGLANYYRRF+EGFSRRA P+TELLKK   W WS + Q AFE+LK 
Sbjct: 875  EWRVPTSVIELRSFLGLANYYRRFIEGFSRRATPMTELLKKGMTWMWSKESQDAFEDLKA 934

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             M +GPVLGL DVTKPFEVETDASD+ALGGVL+Q+ HPI YESRKLN+AERRYTVSEKEM
Sbjct: 935  AMMKGPVLGLADVTKPFEVETDASDYALGGVLLQDDHPIXYESRKLNNAERRYTVSEKEM 994

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSA C+FF+QPKL +KQARWQE LAEFDFKFEHKAG
Sbjct: 995  LAVVHCLRSWRQYLLGSXFVVKTDNSAICHFFNQPKLTSKQARWQELLAEFDFKFEHKAG 1054

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAK 649
            KSNQAADALSRKGEHA LCM AHIH+SK DGS+RD++ E+L   PSA+
Sbjct: 1055 KSNQAADALSRKGEHAXLCMLAHIHTSKADGSIRDLIXEYLQXXPSAR 1102

BLAST of CmoCh13G003730 vs. NCBI nr
Match: TYK07954.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.7e-292
Identity = 487/652 (74.69%), Postives = 567/652 (86.96%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            M+VD  IN +P+KST++DSGATHNFI + EA+RL L   KD G+MKAVNS ALPI+G+ K
Sbjct: 436  MYVDTWINEKPTKSTMVDSGATHNFITEAEAKRLNLRWEKDAGRMKAVNSAALPIIGLVK 495

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            R   ++G W+G +D VVV+MDDFDVVLGMEFLLEH+VIPMPLAKCLVIT   P+V+   +
Sbjct: 496  RTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDL 555

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            +QP  L+MISA+QLK+GL+R++PTFMAIPL     + ETVP+EI  VL+ Y D+MP+SLP
Sbjct: 556  RQPDGLKMISAMQLKKGLSRDEPTFMAIPLNSSENSGETVPKEIVRVLEKYRDVMPDSLP 615

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            ++LPPRR IDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAPYGA 
Sbjct: 616  KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 675

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQKKKDG+LRLCIDYRALNK+TVRNKYPLPII+DLFD+LHGAKYF+KLDLRSGYYQVR
Sbjct: 676  VLFQKKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVR 735

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVF+EYLD+FV+VYLDDIVVY
Sbjct: 736  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVY 795

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STT+EEH+ HL+ VF KL++NQLYVK+EKC+FAQ  INFLGHV+ CG+I M+  KI AI+
Sbjct: 796  STTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKIAAIR 855

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            +W +P SVSELRSFLGLANYYRRFVEGFS+RA+PLTELLKKD  W+W  +CQ AF+ LK 
Sbjct: 856  DWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLTELLKKDVHWNWDPECQAAFDGLKQ 915

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             +  GP+LG+ DVTKPFEVETDASD+ALGGVL+Q GHPI YESRKLN AERRYTVSEKEM
Sbjct: 916  ALMEGPLLGIADVTKPFEVETDASDYALGGVLLQNGHPIAYESRKLNAAERRYTVSEKEM 975

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSATC+FF QPKL +KQARWQE LAEFDF+FEHK G
Sbjct: 976  LAVVHCLRAWRQYLLGSSFVVKTDNSATCHFFTQPKLTSKQARWQEFLAEFDFEFEHKKG 1035

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLLSN 653
             SNQAADALSRK EHAA+C+ AH+  S+I GS+RD ++E L KD +A+ + N
Sbjct: 1036 SSNQAADALSRKQEHAAICLLAHLQGSEIGGSVRDTLREFLQKDHAAQNVMN 1087

BLAST of CmoCh13G003730 vs. NCBI nr
Match: KAA0067557.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 1014.6 bits (2622), Expect = 3.8e-292
Identity = 486/652 (74.54%), Postives = 567/652 (86.96%), Query Frame = 0

Query: 1    MFVDATINSQPSKSTLIDSGATHNFIADQEARRLGLTIGKDPGKMKAVNSEALPIVGVSK 60
            M+VD  IN +P+KST++DSGATHNFI + EA+RL L   KD G+MKAVNS ALPI+G+ K
Sbjct: 462  MYVDTWINQKPTKSTMVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVK 521

Query: 61   RVPFKIGDWTGELDLVVVRMDDFDVVLGMEFLLEHKVIPMPLAKCLVITDRNPTVIHASI 120
            R   ++G W+G +D VVV+MDDFDVVLGMEFLLEH+VIPMPLAKCLVIT   P+V+   +
Sbjct: 522  RTMIRLGGWSGLVDFVVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDL 581

Query: 121  KQPGNLRMISAIQLKRGLAREKPTFMAIPLIEEATTEETVPEEIKEVLDSYTDIMPESLP 180
            +QP  L+MISA+QLK+GL+R++PTFMAIPL     + ETVP+EI  VL+ Y D+MP+SLP
Sbjct: 582  RQPDGLKMISAMQLKKGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLP 641

Query: 181  QTLPPRRGIDHEIKLLPGVKPPAKNAYRMAPPELAELRKQLDELLKAGFIRPAKAPYGAL 240
            ++LPPRR IDHEI+L+PG KPPAKNAYRMAPPELAELRKQLDELL AGFIRPAKAPYGA 
Sbjct: 642  KSLPPRRMIDHEIELVPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAP 701

Query: 241  ILFQKKKDGTLRLCIDYRALNKVTVRNKYPLPIISDLFDQLHGAKYFTKLDLRSGYYQVR 300
            +LFQ+KKDG+LRLCIDYRALNK+TVRNKYPLPII+DLFD+LHGAKYF+KLDLRSGYYQVR
Sbjct: 702  VLFQRKKDGSLRLCIDYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVR 761

Query: 301  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFYEYLDQFVIVYLDDIVVY 360
            IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVF+EYLD+FV+VYLDDIVVY
Sbjct: 762  IAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVY 821

Query: 361  STTLEEHKVHLKLVFDKLRQNQLYVKKEKCAFAQTCINFLGHVVRCGQISMDSDKIKAIQ 420
            STT+EEH+ HL+ VF KL++NQLYVK+EKC+FAQ  INFLGHV+ CG+I M+  KI AI+
Sbjct: 822  STTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKIAAIR 881

Query: 421  EWKVPTSVSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKT 480
            +W +P SVSELRSFLGLANYYRRFVEGFS+RA+PLTELLKKD  W+W  +CQ AF+ LK 
Sbjct: 882  DWAMPKSVSELRSFLGLANYYRRFVEGFSKRASPLTELLKKDVHWNWDPECQTAFDGLKQ 941

Query: 481  TMTRGPVLGLVDVTKPFEVETDASDFALGGVLIQEGHPITYESRKLNDAERRYTVSEKEM 540
             +  GP+LG+ DVTKPFEVETDASD+ALGGVL+Q GHPI YESRKLN AERRYTVSEKEM
Sbjct: 942  ALMEGPLLGIADVTKPFEVETDASDYALGGVLLQNGHPIAYESRKLNAAERRYTVSEKEM 1001

Query: 541  QAVVHCLRVWRQYLLGSQFVVKTDNSATCYFFDQPKLIAKQARWQESLAEFDFKFEHKAG 600
             AVVHCLR WRQYLLGS FVVKTDNSATC+FF QPKL +KQARWQE LAEFDF+FEHK G
Sbjct: 1002 LAVVHCLRAWRQYLLGSSFVVKTDNSATCHFFTQPKLTSKQARWQEFLAEFDFEFEHKKG 1061

Query: 601  KSNQAADALSRKGEHAALCMFAHIHSSKIDGSMRDIMKEHLHKDPSAKLLSN 653
             SNQAADALSRK EHAA+C+ AH+  S+I GS+RD ++E L KD +A+ + N
Sbjct: 1062 SSNQAADALSRKQEHAAICLLAHLQGSEIGGSVRDTLREFLQKDHAAQNVMN 1113

BLAST of CmoCh13G003730 vs. TAIR 10
Match: ATMG00860.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 1.2e-22
Identity = 55/130 (42.31%), Postives = 77/130 (59.23%), Query Frame = 0

Query: 370 HLKLVFDKLRQNQLYVKKEKCAFAQTCINFLG--HVVRCGQISMDSDKIKAIQEWKVPTS 429
           HL +V     Q+Q Y  ++KCAF Q  I +LG  H++    +S D  K++A+  W  P +
Sbjct: 3   HLGMVLQIWEQHQFYANRKKCAFGQPQIAYLGHRHIISGEGVSADPAKLEAMVGWPEPKN 62

Query: 430 VSELRSFLGLANYYRRFVEGFSRRAAPLTELLKKDHPWSWSNDCQMAFENLKTTMTRGPV 489
            +ELR FLGL  YYRRFV+ + +   PLTELLKK +   W+    +AF+ LK  +T  PV
Sbjct: 63  TTELRGFLGLTGYYRRFVKNYGKIVRPLTELLKK-NSLKWTEMAALAFKALKGAVTTLPV 122

Query: 490 LGLVDVTKPF 498
           L L D+  PF
Sbjct: 123 LALPDLKLPF 131

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P043231.3e-8540.55Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
P208259.4e-8440.05Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste... [more]
P0CT411.1e-8135.54Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT341.1e-8135.54Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
P0CT351.1e-8135.54Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
A0A6J1D9060.0e+0080.56Reverse transcriptase OS=Momordica charantia OX=3673 GN=LOC111018360 PE=4 SV=1[more]
A0A5D3C9P88.3e-29374.69Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26... [more]
A0A5D3C4R11.8e-29274.54Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11... [more]
A0A5D3BRZ61.8e-29274.54Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11... [more]
A0A5A7T0E21.8e-29274.54Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20... [more]
Match NameE-valueIdentityDescription
XP_023537907.10.0e+0097.53uncharacterized protein LOC111798805 [Cucurbita pepo subsp. pepo][more]
XP_023524533.10.0e+0097.08uncharacterized protein LOC111788429 [Cucurbita pepo subsp. pepo][more]
XP_022150099.10.0e+0080.56uncharacterized protein LOC111018360 [Momordica charantia][more]
TYK07954.11.7e-29274.69reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0067557.13.8e-29274.54reverse transcriptase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
ATMG00860.11.2e-2242.31DNA/RNA polymerases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.10.10HIV Type 1 Reverse Transcriptase, subunit A, domain 1coord: 189..329
e-value: 1.2E-87
score: 294.6
NoneNo IPR availablePFAMPF08284RVP_2coord: 7..97
e-value: 3.7E-11
score: 43.0
NoneNo IPR availablePANTHERPTHR24559:SF324TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEINcoord: 203..594
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 203..594
NoneNo IPR availableCDDcd00303retropepsin_likecoord: 3..92
e-value: 1.79656E-19
score: 81.6139
NoneNo IPR availableCDDcd09274RNase_HI_RT_Ty3coord: 498..612
e-value: 8.6007E-55
score: 180.766
NoneNo IPR availableCDDcd01647RT_LTRcoord: 228..404
e-value: 1.65797E-88
score: 271.391
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 1..119
e-value: 1.2E-19
score: 72.4
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 2..97
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 413..505
e-value: 8.5E-30
score: 104.6
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 269..404
e-value: 1.2E-87
score: 294.6
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 245..403
e-value: 4.0E-29
score: 101.7
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 224..404
score: 14.533341
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 467..561
e-value: 5.9E-31
score: 106.4
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 167..598

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G003730.1CmoCh13G003730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006508 proteolysis
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008270 zinc ion binding