CmoCh13G000310 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G000310
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein DOUBLE-STRAND BREAK FORMATION
LocationCmo_Chr13: 211141 .. 213118 (-)
RNA-Seq ExpressionCmoCh13G000310
SyntenyCmoCh13G000310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGTGTTCAGTTGCGGAGCAATACTCTCTCTTTTGTTCACGGCTTAGAAGCCGAAGGTTTGGCTATTCACTCTTAATTCTAGCTCATTCATTCGAATTTCCATTGATAATCTTCCGTATATTGTCGATTGTTACATTTTCTTTCATGCTTTATCGCTTATGATTTCCACAGATTAGATGATTCTACTCTGCGAATTCTGGAATTCTTTTCCGCTTCCAAAGACACGATGTCGTTGATGGATGTCAAATCCGGAGTAAAAGAATTACTCAGCTTTGAATCTCTATCTATCATTCGTGAAACTGTTGAGAAAACTGATGATCAAAAGCTTCTAGTCATCGAGTTTCTTGTTCGAGCTTTTGCTCTTGTTGGAGACATTGAGGCTAGTATTCATTTGCATAAGTCTCTGTGATCGTTCAGAACATCAGAAATTGTGCTTTTAGATTAGTCTTTTTTGTTGTTATTTTGTTTAGTCTAAATATGGTATCGTGTTAATAAAACCGTGGTTATTCACTTTTGCTTGCTATCTAAGTTTTGGTGCTTTCTAAGATTTCATCGTGGGAGCACCGCAACATTTCCCCTCCTTAAATCACCTAGTTTTGGTATTTATATTCTATGCTAAATGATGAACTTGTTAGCAGATGTGACTACCTATTTCTCTAAGTTCAGCTTACACTTCTTATGCTTCTAGAGTTGCTTAGCTTTGAGATATGAGGCCTTGAATTTTCGGGAACTGAAGTCTTTTAATCAACCAAGGCTTCAAGTCTCACACGCGGAATGGTTAAACTTCGCTGAGCATTCATTAAACGCTGGCTTTTTTTCAATTGCTATAAAGGTAAGGTAATTTCTCCTATTTATGCAACTGCTTAAGATTTTCTTTAAAACTTTGGTTAGGTAGAGGAGCCTGTCCATTAAAAGTTCTATATATTATGTGTGTTGGATCCCTTATGTAGCTAAGCATATTGAGTATGTGAAATGTGAAGTGAGATTAAATTCTTAGTCGAGATGTATTTGTCTTTCGGGAAATTAAGAATCGAGCTCTACAATTTTGGAGATTTGCGGAATGGTGGTGCTAGTAGTTCATAAGGAAAAGCTTTGAGTGGAGAATTCTTAAATAGGAAAGAATAGCTCGGCATAAACGCTAATATTGTGGCTAAGTTTTAAGAAAGAGGAAGAGAATATCATCAAATGCATGTGGAATGCATGTGAACTGCACATGATCCAATGTAACTAACTTGAGGTGCCTAATATACAATATCAGAAGAAAATATGATGCATGACATTACAACAAATCCTATTGCATAACGTTCTCCTGTTATGAGGTTGGTCATTAAGTAAAGAAAACAATAGGTAAAGAGAAGCTTTGTCAGAATGTTTTCTAGACTAAAAATGGTTTGAATTGAAGAATATGCTTATTTATTACATGCTCACGAACAAAATGATTGTAGGCCTCAACGAGTTTGGAATAAACACTGTTATTATAGCAAAGTAAAATCGATGGCGAGAAAGGAGAAAAACATACTGTCCGTTGCACAACTTCTACCCATTAGTGCAACAACATTAATTTTATTTCTTCCATTCTATCAGGTTAAGTTGGATGTGATCAAATCTAATCTAGATGCACGACTCTGTTTTTCACCGTATACCTATTAACTTTTTGTTTTTGTCATTTTTTTCCATATCTTGTGTCTTATTGTCGTATTTCAGCTCTAATCTTTAGTATTCAGTGGGTTTTTCTCATGATACAAAGAAATGATGGTGAATATTCAATTAGAGTCTCATTCAATACGGTGAACGTATTTACATTTTTTTTTTACAGGCATATGAGCAAGCACTGTCAAGCCTTCAGCAGAGTGATACTGCAAACTACACATCACATGGTTCCTCTAAATGTGCGGAAGTTATTGAGAAGATAAAGAGACTCAAAGATCATGCTCTGAAATCAGCTGGTTCCCATTCTGGTAGGGATCTTAGTTGA

mRNA sequence

ATGCCGTGTTCAGTTGCGGAGCAATACTCTCTCTTTTGTTCACGGCTTAGAAGCCGAAGATTAGATGATTCTACTCTGCGAATTCTGGAATTCTTTTCCGCTTCCAAAGACACGATGTCGTTGATGGATGTCAAATCCGGAGTAAAAGAATTACTCAGCTTTGAATCTCTATCTATCATTCGTGAAACTGTTGAGAAAACTGATGATCAAAAGCTTCTAGTCATCGAGTTTCTTGTTCGAGCTTTTGCTCTTGTTGGAGACATTGAGGCTAGTATTCATTTGCATAATTGCTTAGCTTTGAGATATGAGGCCTTGAATTTTCGGGAACTGAAGTCTTTTAATCAACCAAGGCTTCAAGTCTCACACGCGGAATGGTTAAACTTCGCTGAGCATTCATTAAACGCTGGCTTTTTTTCAATTGCTATAAAGGCATATGAGCAAGCACTGTCAAGCCTTCAGCAGAGTGATACTGCAAACTACACATCACATGGTTCCTCTAAATGTGCGGAAGTTATTGAGAAGATAAAGAGACTCAAAGATCATGCTCTGAAATCAGCTGGTTCCCATTCTGGTAGGGATCTTAGTTGA

Coding sequence (CDS)

ATGCCGTGTTCAGTTGCGGAGCAATACTCTCTCTTTTGTTCACGGCTTAGAAGCCGAAGATTAGATGATTCTACTCTGCGAATTCTGGAATTCTTTTCCGCTTCCAAAGACACGATGTCGTTGATGGATGTCAAATCCGGAGTAAAAGAATTACTCAGCTTTGAATCTCTATCTATCATTCGTGAAACTGTTGAGAAAACTGATGATCAAAAGCTTCTAGTCATCGAGTTTCTTGTTCGAGCTTTTGCTCTTGTTGGAGACATTGAGGCTAGTATTCATTTGCATAATTGCTTAGCTTTGAGATATGAGGCCTTGAATTTTCGGGAACTGAAGTCTTTTAATCAACCAAGGCTTCAAGTCTCACACGCGGAATGGTTAAACTTCGCTGAGCATTCATTAAACGCTGGCTTTTTTTCAATTGCTATAAAGGCATATGAGCAAGCACTGTCAAGCCTTCAGCAGAGTGATACTGCAAACTACACATCACATGGTTCCTCTAAATGTGCGGAAGTTATTGAGAAGATAAAGAGACTCAAAGATCATGCTCTGAAATCAGCTGGTTCCCATTCTGGTAGGGATCTTAGTTGA

Protein sequence

MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSIIRETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQVSHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKDHALKSAGSHSGRDLS
Homology
BLAST of CmoCh13G000310 vs. ExPASy Swiss-Prot
Match: Q8RX33 (Protein DOUBLE-STRAND BREAK FORMATION OS=Arabidopsis thaliana OX=3702 GN=DFO PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.2e-29
Identity = 79/187 (42.25%), Postives = 114/187 (60.96%), Query Frame = 0

Query: 5   VAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSIIRETV 64
           +A+Q  LF +R++ RR D+ +LRILE    + +  S ++V+S +++ +  ES+ I  E  
Sbjct: 32  IADQTYLFINRVQDRRFDEESLRILELSLVAMNVKSFLEVRSRLRDFMRSESVVIFGELT 91

Query: 65  EKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQVSHAE 124
            ++   KL V+EF  RAFAL+GD+E      +CLA+RYEALN R+LKS +   L VSH+E
Sbjct: 92  GESMVAKLSVLEFFARAFALLGDME------SCLAMRYEALNLRQLKSPSCLWLGVSHSE 151

Query: 125 WLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKDHALK 184
           W  FA  S+  GF SIA KA E AL SL++       S  +S   +  EK++RL+D A  
Sbjct: 152 WTKFAVQSMENGFPSIAGKASENALLSLKKDSLIEPKSEDNSDILDAAEKVRRLRDSAAS 211

Query: 185 SAGSHSG 192
              SHSG
Sbjct: 212 LTSSHSG 212

BLAST of CmoCh13G000310 vs. ExPASy TrEMBL
Match: A0A6J1HPP0 (uncharacterized protein LOC111464906 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464906 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.2e-91
Identity = 184/195 (94.36%), Postives = 187/195 (95.90%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETVEKTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           HALKSAGSHS + L+
Sbjct: 181 HALKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. ExPASy TrEMBL
Match: A0A6J1HMC3 (uncharacterized protein LOC111464906 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464906 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.2e-91
Identity = 184/195 (94.36%), Postives = 187/195 (95.90%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETVEKTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           HALKSAGSHS + L+
Sbjct: 181 HALKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. ExPASy TrEMBL
Match: A0A6J1I645 (uncharacterized protein LOC111469552 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469552 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.3e-87
Identity = 179/195 (91.79%), Postives = 182/195 (93.33%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRR DDSTLRILEFFSASKDTM  MDVKSGVKELL FESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRFDDSTLRILEFFSASKDTMYFMDVKSGVKELLRFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETVEKTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSK AEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKRAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           HALKSAGSHS + L+
Sbjct: 181 HALKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. ExPASy TrEMBL
Match: A0A6J1I136 (uncharacterized protein LOC111469552 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469552 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.3e-87
Identity = 179/195 (91.79%), Postives = 182/195 (93.33%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRR DDSTLRILEFFSASKDTM  MDVKSGVKELL FESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRFDDSTLRILEFFSASKDTMYFMDVKSGVKELLRFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETVEKTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSK AEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKRAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           HALKSAGSHS + L+
Sbjct: 181 HALKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. ExPASy TrEMBL
Match: A0A1S3CL48 (uncharacterized protein LOC103502216 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502216 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.8e-71
Identity = 154/187 (82.35%), Postives = 159/187 (85.03%), Query Frame = 0

Query: 9   YSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSIIRETVEKTD 68
           YSLF SRLRSRR DDSTLRILE F ASKD  SL DVKS   ELL FESLSIIRET EKTD
Sbjct: 5   YSLFLSRLRSRRFDDSTLRILELFPASKDATSLADVKSSFSELLRFESLSIIRETAEKTD 64

Query: 69  DQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQVSHAEWLNF 128
           DQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFR LKSFNQP LQVSHAEWLNF
Sbjct: 65  DQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRVLKSFNQPWLQVSHAEWLNF 124

Query: 129 AEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKDHALKSAGS 188
           AEHSL+AGFFSIAIKAYEQALSSLQQSDTANYTSHGS K  EVIEKI RLKDHAL  +GS
Sbjct: 125 AEHSLHAGFFSIAIKAYEQALSSLQQSDTANYTSHGSFKHTEVIEKINRLKDHALNLSGS 184

Query: 189 HSGRDLS 196
           HS + L+
Sbjct: 185 HSVQALT 185

BLAST of CmoCh13G000310 vs. NCBI nr
Match: XP_022964954.1 (uncharacterized protein LOC111464906 isoform X1 [Cucurbita moschata])

HSP 1 Score: 344.4 bits (882), Expect = 6.6e-91
Identity = 184/195 (94.36%), Postives = 187/195 (95.90%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETVEKTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           HALKSAGSHS + L+
Sbjct: 181 HALKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. NCBI nr
Match: XP_022964955.1 (uncharacterized protein LOC111464906 isoform X2 [Cucurbita moschata])

HSP 1 Score: 344.4 bits (882), Expect = 6.6e-91
Identity = 184/195 (94.36%), Postives = 187/195 (95.90%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETVEKTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           HALKSAGSHS + L+
Sbjct: 181 HALKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. NCBI nr
Match: XP_023520164.1 (uncharacterized protein LOC111783465 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.4 bits (864), Expect = 8.1e-89
Identity = 179/195 (91.79%), Postives = 185/195 (94.87%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRR DDSTLRILEFFSASKDTMSLMDVKSGVKELL FESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRFDDSTLRILEFFSASKDTMSLMDVKSGVKELLRFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETV+KTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVDKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIA+KAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAMKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           H+LKSAGSHS + L+
Sbjct: 181 HSLKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. NCBI nr
Match: XP_023520165.1 (uncharacterized protein LOC111783465 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520166.1 uncharacterized protein LOC111783467 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.4 bits (864), Expect = 8.1e-89
Identity = 179/195 (91.79%), Postives = 185/195 (94.87%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRR DDSTLRILEFFSASKDTMSLMDVKSGVKELL FESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRFDDSTLRILEFFSASKDTMSLMDVKSGVKELLRFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQV 120
           RETV+KTDDQKLLVIEFLVRAFALVGDIE      +CLALRYEALNFRELKSFNQPRLQV
Sbjct: 61  RETVDKTDDQKLLVIEFLVRAFALVGDIE------SCLALRYEALNFRELKSFNQPRLQV 120

Query: 121 SHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180
           SHAEWLNFAEHSLNAGFFSIA+KAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD
Sbjct: 121 SHAEWLNFAEHSLNAGFFSIAMKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKD 180

Query: 181 HALKSAGSHSGRDLS 196
           H+LKSAGSHS + L+
Sbjct: 181 HSLKSAGSHSVQALT 189

BLAST of CmoCh13G000310 vs. NCBI nr
Match: KAG6583341.1 (Protein DOUBLE-STRAND BREAK FORMATION, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 336.3 bits (861), Expect = 1.8e-88
Identity = 184/208 (88.46%), Postives = 188/208 (90.38%), Query Frame = 0

Query: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSII 60
           MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELL FESLSII
Sbjct: 1   MPCSVAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLRFESLSII 60

Query: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEASI------------HL-HNCLALRYEALNF 120
           RETVEKTDDQKLLVIEFLVRAFALVGDIEA+             HL  +CLALRYEALNF
Sbjct: 61  RETVEKTDDQKLLVIEFLVRAFALVGDIEANFIVGAPQHFPSLNHLVLSCLALRYEALNF 120

Query: 121 RELKSFNQPRLQVSHAEWLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSK 180
           RELKSFNQPRLQVSHAEWLNFAEHSLN GFFSIAIKAYEQALSSLQQSDTANYTSH SSK
Sbjct: 121 RELKSFNQPRLQVSHAEWLNFAEHSLNTGFFSIAIKAYEQALSSLQQSDTANYTSHDSSK 180

Query: 181 CAEVIEKIKRLKDHALKSAGSHSGRDLS 196
           CAEVIEKIKRLKDHALKSAGSHS + L+
Sbjct: 181 CAEVIEKIKRLKDHALKSAGSHSVQALT 208

BLAST of CmoCh13G000310 vs. TAIR 10
Match: AT1G07060.1 (unknown protein; Has 30 Blast hits to 30 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 30; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 131.3 bits (329), Expect = 8.2e-31
Identity = 79/187 (42.25%), Postives = 114/187 (60.96%), Query Frame = 0

Query: 5   VAEQYSLFCSRLRSRRLDDSTLRILEFFSASKDTMSLMDVKSGVKELLSFESLSIIRETV 64
           +A+Q  LF +R++ RR D+ +LRILE    + +  S ++V+S +++ +  ES+ I  E  
Sbjct: 32  IADQTYLFINRVQDRRFDEESLRILELSLVAMNVKSFLEVRSRLRDFMRSESVVIFGELT 91

Query: 65  EKTDDQKLLVIEFLVRAFALVGDIEASIHLHNCLALRYEALNFRELKSFNQPRLQVSHAE 124
            ++   KL V+EF  RAFAL+GD+E      +CLA+RYEALN R+LKS +   L VSH+E
Sbjct: 92  GESMVAKLSVLEFFARAFALLGDME------SCLAMRYEALNLRQLKSPSCLWLGVSHSE 151

Query: 125 WLNFAEHSLNAGFFSIAIKAYEQALSSLQQSDTANYTSHGSSKCAEVIEKIKRLKDHALK 184
           W  FA  S+  GF SIA KA E AL SL++       S  +S   +  EK++RL+D A  
Sbjct: 152 WTKFAVQSMENGFPSIAGKASENALLSLKKDSLIEPKSEDNSDILDAAEKVRRLRDSAAS 211

Query: 185 SAGSHSG 192
              SHSG
Sbjct: 212 LTSSHSG 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RX331.2e-2942.25Protein DOUBLE-STRAND BREAK FORMATION OS=Arabidopsis thaliana OX=3702 GN=DFO PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1HPP03.2e-9194.36uncharacterized protein LOC111464906 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1HMC33.2e-9194.36uncharacterized protein LOC111464906 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1I6451.3e-8791.79uncharacterized protein LOC111469552 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1I1361.3e-8791.79uncharacterized protein LOC111469552 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A1S3CL482.8e-7182.35uncharacterized protein LOC103502216 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
XP_022964954.16.6e-9194.36uncharacterized protein LOC111464906 isoform X1 [Cucurbita moschata][more]
XP_022964955.16.6e-9194.36uncharacterized protein LOC111464906 isoform X2 [Cucurbita moschata][more]
XP_023520164.18.1e-8991.79uncharacterized protein LOC111783465 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023520165.18.1e-8991.79uncharacterized protein LOC111783465 isoform X2 [Cucurbita pepo subsp. pepo] >XP... [more]
KAG6583341.11.8e-8888.46Protein DOUBLE-STRAND BREAK FORMATION, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
AT1G07060.18.2e-3142.25unknown protein; Has 30 Blast hits to 30 proteins in 10 species: Archae - 0; Bac... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044969Protein DOUBLE-STRAND BREAK FORMATIONPANTHERPTHR37176F10K1.23coord: 3..190

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G000310.1CmoCh13G000310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042138 meiotic DNA double-strand break formation