CmoCh12G013200 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G013200
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	vacuolar protein sorting-associated protein 32 homolog 2
Location	Cmo_Chr12: 11545514 .. 11549086 (+)
RNA-Seq Expression	CmoCh12G013200
Synteny	CmoCh12G013200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGGAACCAATTAAATACATTGCCGCGTGTGGTGGACAAACAGCGACGGGAAGAGAGGGAACTCCAAATCTTCGTCCTTCCTGTTCTGGAGCGCCTTGGAAGAAACCCTTTCTACTTCAAATTTCTTCACAAATTCTTTCATTTCATTCTGGTTGGTATAAATCTGTCTCTGATTATCATCTTTCCGTCTCAATCTCTCGCTGGTTTCTCGATTCCTTTCTTGTTGGCATTGATTTTTTTTTTTTGAATCGTATCGAAGTTGATCGGCAGGGCGATTTCTCTTCTTGAGGAATTCATTTTGTCTTTGATTTCGTTGTTGCAGATCGAGGGGCGATTCTTTTTTCTTAGCTTAAACAATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAATGAGGTATGTTTTACTTCGATGTTTTATTGTTTTTGATATTCTTTGTATGGAAATGATCTTTTCCTTTTCTCACTTGTTCTGAACCTATTTCATCGATCTTGATGGATTCTTGGCTTCTCGATCATTCCGTTTTTTCCTCGTCTTTTCCAAAAATTAGTGTATATGACGGGGTAGTAATTGGTTCTACTTGACGGAGTATTTGTGTTTATGACGTCGTATTAACTTGTTCTCATTGATAGAGTACTAGTGTTTATGACGTCGTAGTAATTGGTTCTCCTTGACCGAGTATTAGTGTTTATAATGTCGTAGTTATTGGTTTCCAGTTGAAATTATCGTCTTATTTGGATTTTTTAACTCGTAATTTTCCAGTTGAAATGTCGTAGTTACTGCCGTTCTTTAGGTGAAATTTCGCTTATGTAAGAAAGGAAACCTGTGTACTACAATGCTGCCGTTCTTTGGAATTGCGTCTAAAATTGTGGTTGCGTAGATTTTTGTAGTCCGACTAAGCTTTTCTTAATTGATACCGATATTTTTGTGCACTTCATTGTCCGGCGTTGTTTGATTTAATTGCTTCCTTTTCATCGTCTGGGATTATCTGTTTAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGGTGTGTTATGTAATTGGGTGATCTCTTCTATGGTCAAATGCTTCTTGGTATTAATTTTTATTCCTTTTTTGAGTATTTGTTCACATGTGAAGTGTGAATAAGATTTAATTAACCCCCGTTTTCTCCGCTCTGGAGTGTTAATTTGATGGTACTGCCATTCTATGGTCAAATGCAAGTTGTGCTTATGTCTGTATATTGCAATGTGATACTCCGCAGAATTTATTACTGAATCCTCCATCTACTTTTTTTTGTTCATTTCCTTATTAGGAAAATCTTGTGCTTGATGAATTATTCTACTCTACATCCTCAATTGATGAAGTAGCCTCGTGCATTTTTTGGAGGATGATTTTTCTTGTGTTTTGTTGCCAGTCTTTGTGACTGATGGTTGCAATGTATCCCAATATTTTTTGTTGTAGTTTATGATGGCCATACATTTGTAGCTTTGTTTATTTCAAATCTTCGTTTGAGCTTTCTTTCATTTTTGGAGTTTTGTGATTTTCCTTGTTCTTATCTTTATCAGTGTTTTGGATATCACTTTCAAATGGGTTTTAATTTTATATTTCATGTCTATTTTAGTCTGAGCTGCATTTTTGGAGTTTATTCTGTCTGCTTTTTTGGGCTCTATAGTTTGTTCCTTTTCATTCTTGTTGATTTATTTAATATTTTTAAAATCATTGTAATTTTTGCTGTTCCCTATCCTTCTATCTCTATATTACCTCGTGTTCTTATTTGCCAACCCATTTGGATCGTTTTGTTTATTGGATATGAATATCAAATATTAGGATTAAATGTCTAGGATATGAAATAGTTCTGTTTATAACTCGAACATTTGTTCTTTACAAGAATTGTTGTTTATAGGTTTCCTTCCTGACAATTTAATTAGTTTAATCAATTAGTTAGATCTGAAAGAAAAAATTAATGATATTAAATTGAAAGATTCGATTCATAGTCATTAGAATTGGTTGCAGCGGCCATACAATGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGGTGAGCTGGAGTCGTTATTTACTGATTGATGAGACATTTATTTCTTGTACTTTCAGTTAACTGTATTGAAATCAACCTTCTACTTTATTAACAATTTTTTAAACTTTTGCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACGTATGCCTAATATTTATTTGATTTGCTTTCTTTCTGCCTAACATTCTTTTTGTCTTTACAATCGATCTTTTAGCACAACTATTAACATTACTATTGCCTAAGCCGGAAAATGAAAAAAAAAAAAAAAAAAAACTGTACAAAATATTGCTCAACTTATTTATGCACTTCGTTCATCTAGTTATTGATGTTAAGCTAACCAATCTTGAGAAAGTTAGCCATTACCATTAAGCATTACTTGCAATGGCAACTGAAAAATGAAATAATTTGTGAAAGTTTGTTTCATGTTTTATTTCTTTAACCGAGGTTGTGAAATTTGGAACCGTACTTATTTTAAAAATGTAGGCTGATTATTTGAAACTTTCTTCTCCAGAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAGGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGTACTTCATCTTTGGCTTGTTTCTTGGCCTTTCCTGGTATTTGCTTATGTCGCACAATTCATCTATTAACAATGGTGGTGTAATCAGGATGAATTGGAGGCAGAACTGGAAGAGCTTGAAAGTGCTGAGTTGGAAGAACAACTTCTCCAGCCAGCCTCAACGGCTCCTGCTGGACCTGTCTCAGTCCCGTCAGGCCGAGTTCCTGCTCGCCCTGTTCCACAGAAGAGAACTGCGGAAGAAGACGAGCTGGCAGCTCTGCAAGCTGAGATGGCCCTCTAACCAGGTTTGTTCCGTAGCCCAATCTTAAGCTGAATGAGCTCCGATTAACTTTCTATAATCACACTGTTCAAGTCCAAGTACTCTAAATTTGTAGTTGAACGTTGTTTTATCTTAAAAGATTACCTTTTATGGAGCAGAGAGCTGAATTTTAATTGGACATTGCAGGTGGTGCATACGAGTATCATCTTCTGGCAGATTGCATCCGAATCTTCTAAGCGTTTGATTGAAATTTTACACAAATTACCCTTCCCTGCGGCAAGACAATGTATATATAAAGGTGCAAAATTACTGGGATCTTCATCTTAAAATCATCTAAAAATCTTATACGAATCTTCAATTTTAAATTATTGGGAAATCTAGGTAATATTTGAGTAATTATAATTTCTTTGACTTATAATAAAAATGGCATGTTTGTTTACTATA

mRNA sequence

CGGGAACCAATTAAATACATTGCCGCGTGTGGTGGACAAACAGCGACGGGAAGAGAGGGAACTCCAAATCTTCGTCCTTCCTGTTCTGGAGCGCCTTGGAAGAAACCCTTTCTACTTCAAATTTCTTCACAAATTCTTTCATTTCATTCTGATCGAGGGGCGATTCTTTTTTCTTAGCTTAAACAATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAATGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAGGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTGGAAGAGCTTGAAAGTGCTGAGTTGGAAGAACAACTTCTCCAGCCAGCCTCAACGGCTCCTGCTGGACCTGTCTCAGTCCCGTCAGGCCGAGTTCCTGCTCGCCCTGTTCCACAGAAGAGAACTGCGGAAGAAGACGAGCTGGCAGCTCTGCAAGCTGAGATGGCCCTCTAACCAGGTGGTGCATACGAGTATCATCTTCTGGCAGATTGCATCCGAATCTTCTAAGCGTTTGATTGAAATTTTACACAAATTACCCTTCCCTGCGGCAAGACAATGTATATATAAAGGTGCAAAATTACTGGGATCTTCATCTTAAAATCATCTAAAAATCTTATACGAATCTTCAATTTTAAATTATTGGGAAATCTAGGTAATATTTGAGTAATTATAATTTCTTTGACTTATAATAAAAATGGCATGTTTGTTTACTATA

Coding sequence (CDS)

ATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAATGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAGGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTGGAAGAGCTTGAAAGTGCTGAGTTGGAAGAACAACTTCTCCAGCCAGCCTCAACGGCTCCTGCTGGACCTGTCTCAGTCCCGTCAGGCCGAGTTCCTGCTCGCCCTGTTCCACAGAAGAGAACTGCGGAAGAAGACGAGCTGGCAGCTCTGCAAGCTGAGATGGCCCTCTAA

Protein sequence

MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL

Homology

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BLAST of CmoCh12G013200 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-95
Identity = 195/219 (89.04%), Postives = 206/219 (94.06%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAGP-VSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           AP  P V VP+GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

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BLAST of CmoCh12G013200 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 1.5e-86
Identity = 185/218 (84.86%), Postives = 192/218 (88.07%), Query Frame = 0


Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +NRLFGKPKQE + L TLDKLNETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQ
Sbjct: 3   MNRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQ 62

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
           CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD
Sbjct: 63  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 122

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTA 181
           VDKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AELEEQLLQP    
Sbjct: 123 VDKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQPV--- 182

Query: 182 PAGPVSVPSGRVPARPVPQKR-TAEEDELAALQAEMAL 219
              P+ VP G  PAR   QK+ TAEEDELAALQAEMAL
Sbjct: 183 ---PIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

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BLAST of CmoCh12G013200 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.1e-27
Identity = 87/208 (41.83%), Postives = 121/208 (58.17%), Query Frame = 0


Query: 7   GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRK 66
           GK  + A+    + +L ET EML KK+  L KK   E+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKRAALQALKRK 71

Query: 67  RLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     +ID VD+ M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNMDIDKVDELM 131

Query: 127 DEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAGPV 186
            +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LL+     P    
Sbjct: 132 QDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLLEIGDNVPL--P 191

Query: 187 SVPSGRVPARPVPQKRTAEEDELAALQA 215
           +VPS  +P+RP  +K   +ED++  L+A
Sbjct: 192 NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

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BLAST of CmoCh12G013200 vs. ExPASy Swiss-Prot
Match: Q6IQ73 (Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.3e-26
Identity = 90/208 (43.27%), Postives = 118/208 (56.73%), Query Frame = 0


Query: 7   GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRK 66
           GK  +       + KL ET EML KK++ L KK  AE+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGGKGPTPQEAIQKLRETEEMLAKKQDFLEKKIDAELLIAKK-NGTKNKRAALQALKRK 71

Query: 67  RLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMK   +  +ID VD  M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKTAHENMDIDKVDDLM 131

Query: 127 DEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAGPV 186
            +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LLQ      +GP 
Sbjct: 132 QDITEQQELAQEISDAISRPVGFGEEFDEDELMAELEELEQEELDKDLLQ-----ISGPE 191

Query: 187 SVPSGRVPARPVP------QKRTAEEDE 209
            VP   VP+ P+P      QK+  EEDE
Sbjct: 192 DVPLPNVPSNPLPKKTAVAQKKRQEEDE 213

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BLAST of CmoCh12G013200 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-26
Identity = 84/216 (38.89%), Postives = 125/216 (57.87%), Query Frame = 0


Query: 1   MLNRLF---GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKR 60
           ++ +LF   GK  +  +    + KL +T EML KK+  L KK   E+  AK+    KNKR
Sbjct: 3   LIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKNKR 62

Query: 61  AAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQP 180
           +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL+ 
Sbjct: 123 DIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLLEV 182

Query: 181 ASTAPAGPVSVPSGRVPARPVPQKRTAEEDELAALQ 214
                    +VP+  +PA+PV +K+  ++D++  L+
Sbjct: 183 QGPETVPLPNVPAASLPAKPVKKKQEEDDDDMRELE 217

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BLAST of CmoCh12G013200 vs. ExPASy TrEMBL
Match: A0A6J1FBK1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444128 PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 5.1e-106
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

[Close]

BLAST of CmoCh12G013200 vs. ExPASy TrEMBL
Match: A0A6J1HQ32 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita maxima OX=3661 GN=LOC111465657 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 7.4e-105
Identity = 217/218 (99.54%), Postives = 217/218 (99.54%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APAGPVSV SGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 218

[Close]

BLAST of CmoCh12G013200 vs. ExPASy TrEMBL
Match: A0A6J1G2S3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450291 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.9e-102
Identity = 208/218 (95.41%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PA PVS+PSGRVPARPVPQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

[Close]

BLAST of CmoCh12G013200 vs. ExPASy TrEMBL
Match: A0A1S3CAQ7 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103498351 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.7e-101
Identity = 207/218 (94.95%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APA PVSVP+GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

[Close]

BLAST of CmoCh12G013200 vs. ExPASy TrEMBL
Match: A0A5A7V4V7 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00800 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.7e-101
Identity = 207/218 (94.95%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APA PVSVP+GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

[Close]

BLAST of CmoCh12G013200 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 349.7 bits (896), Expect = 1.6e-96
Identity = 195/219 (89.04%), Postives = 206/219 (94.06%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAGP-VSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           AP  P V VP+GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

[Close]

BLAST of CmoCh12G013200 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 349.7 bits (896), Expect = 1.6e-96
Identity = 195/219 (89.04%), Postives = 206/219 (94.06%), Query Frame = 0


Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAGP-VSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           AP  P V VP+GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

[Close]

BLAST of CmoCh12G013200 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 320.5 bits (820), Expect = 1.1e-87
Identity = 185/218 (84.86%), Postives = 192/218 (88.07%), Query Frame = 0


Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +NRLFGKPKQE + L TLDKLNETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQ
Sbjct: 3   MNRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQ 62

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
           CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD
Sbjct: 63  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 122

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTA 181
           VDKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AELEEQLLQP    
Sbjct: 123 VDKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQPV--- 182

Query: 182 PAGPVSVPSGRVPARPVPQKR-TAEEDELAALQAEMAL 219
              P+ VP G  PAR   QK+ TAEEDELAALQAEMAL
Sbjct: 183 ---PIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

[Close]

BLAST of CmoCh12G013200 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 301.6 bits (771), Expect = 5.1e-82
Identity = 170/192 (88.54%), Postives = 180/192 (93.75%), Query Frame = 0


Query: 28  MLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQM 87
           MLEKKE VL+KKA AEVEKAKE++R KNKRAAIQCLKRKRLYE QVEQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  IMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPI 147
           IMLEGAKATTETVDALR+GASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+TP+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GAAADFDEDELEAELEELESAELEEQLLQPASTAPAGP-VSVPSGRVPARPVPQKRTAEE 207
           GAAADFDEDEL AEL+ELES ELE QLLQPA+TAP  P V VP+GR PARPVPQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 208 DELAALQAEMAL 219
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

[Close]

BLAST of CmoCh12G013200 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 55.8 bits (133), Expect = 4.9e-08
Identity = 58/206 (28.16%), Postives = 96/206 (46.60%), Query Frame = 0


Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +  LF K  +       +  L      L + +  L K   AE + A++  R K K  A+ 
Sbjct: 1   MGNLFVKKPKITEVDRAILSLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
            LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G +A+KA+Q   N+DD
Sbjct: 61  ALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLDD 120

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADF-DEDELEAELEELESAELEEQLLQPAST 181
           V K MD+    T   K  Q+ LS  +G      DE+E+ AE + LES  + E + +  +T
Sbjct: 121 VQKLMDD----TAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPTT 180

Query: 182 --APAGPVSVPSGRVPAR-PVPQKRT 204
              P  P  +    VP + PV    T
Sbjct: 181 ELMPEEPEKMDLPDVPTKAPVASNET 202

The following BLAST results are available for this feature:

BLAST of CmoCh12G013200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZE4	2.3e-95	89.04	Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana...	[more]
O82197	1.5e-86	84.86	Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana...	[more]
Q7ZVC4	2.1e-27	41.83	Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=...	[more]
Q6IQ73	1.3e-26	43.27	Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=...	[more]
Q5XGW6	3.9e-26	38.89	Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ...	[more]

BLAST of CmoCh12G013200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FBK1	5.1e-106	100.00	vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata O...	[more]
A0A6J1HQ32	7.4e-105	99.54	vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita maxima OX=...	[more]
A0A6J1G2S3	5.9e-102	95.41	vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch...	[more]
A0A1S3CAQ7	1.7e-101	94.95	vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656...	[more]
A0A5A7V4V7	1.7e-101	94.95	Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va...	[more]

BLAST of CmoCh12G013200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29160.1	1.6e-96	89.04	SNF7 family protein	[more]
AT4G29160.3	1.6e-96	89.04	SNF7 family protein	[more]
AT2G19830.1	1.1e-87	84.86	SNF7 family protein	[more]
AT4G29160.2	5.1e-82	88.54	SNF7 family protein	[more]
AT5G09260.1	4.9e-08	28.16	vacuolar protein sorting-associated protein 20.2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 12..39
None	No IPR available	COILS	Coil	Coil	coord: 122..142
None	No IPR available	COILS	Coil	Coil	coord: 151..175
None	No IPR available	GENE3D	1.10.287.1060		coord: 14..91 e-value: 2.4E-22 score: 80.8
None	No IPR available	GENE3D	6.10.250.1710		coord: 117..171 e-value: 3.0E-9 score: 38.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..218
None	No IPR available	PANTHER	PTHR22761	CHARGED MULTIVESICULAR BODY PROTEIN	coord: 1..218
None	No IPR available	PANTHER	PTHR22761:SF63	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 32 HOMOLOG 1	coord: 1..218
IPR005024	Snf7 family	PFAM	PF03357	Snf7	coord: 20..185 e-value: 2.9E-46 score: 157.2

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G013200.1	CmoCh12G013200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway
biological_process	GO:0006900	vesicle budding from membrane
biological_process	GO:0007034	vacuolar transport
cellular_component	GO:0009898	cytoplasmic side of plasma membrane
cellular_component	GO:0000815	ESCRT III complex
cellular_component	GO:0005771	multivesicular body