CmoCh12G006320 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G006320
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	Cmo_Chr12: 3991561 .. 3993159 (-)
RNA-Seq Expression	CmoCh12G006320
Synteny	CmoCh12G006320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCTCAAGGACTTGATAAGCGTCCATTGAAAGAGTTGGTAGCTGAGATTAGTACTCGCAAATTCAATTGTGTTCGATTGACGTGGTCTGTCCATATGTTTACTCGTTATGCATATGAGACTATTGGTGATGTCTTTGACGGTCTAGATATTGCTGACGTAAAAAATGGAGTTAAAAAGCACAATCCTGAAATATTGAAAATGACGGTTGCTAAGGCGTTTCAAACTGTCATTAATGCTTTGGGCTCGGAGAAAATTATGGTTATTTTAGATAATCATATTAGCCAACCAAGATGGTGTTGCTCTCTCGACGATGGAAATGGGTTCTTTGGAGATTGCAATTTTAACCCTAGTGAATGGCTTCAAAGCTTGTCATTTGTTGCCGTGCAATTCACATGCAATCCATATGTGAGTTTGACTTTCTAAACTTTTTTTTTTTTAAATGAAAATTTCGTTATTATTATTAATATTTTGAATTTTTACGATCCTTTTTGTGTTAAAGGTGGTAGGAATGAGTTTAAGAAACGAGCTTCGAGGGTCATTATCAAATGCTGATGCCTGGTGCTATTATGTGAGACAAGGAAGCCAACTGATCCATAGAATAAACCCTAATCTCCTTGTAGTCATTTCTGGACTAAACTATGACAATGATCTAAGCCATCTAAAGAAAAGACCTCTAGGCTACACTTTACACAACAAAGTAGTGTTGGAGGCACATCTCTACTCGTTCAGTGGAGATCATGAAAGCAAGTTCACAAAGAATCCATTAAATGTAATATGCGACCAAATCATGGAGAAGTTTGAAAAGGAAGCTGGGTTTGTGGTGGACATGGAGAATCCATATCCTTTGTTCCTTAGTGAATTCGGGTACGATCAATCGGGTGGAAATGATGCTCAAAATCGATTCATGAGCTGCTTTTTAGCTCGAATTGTAGAGAAGGATATTGATTGGGCGTTGTGGGCTTTCCAAGGAAGTTATATGTATAGACAAGGACATCAAGATATTGAAGAAAGTTTTGGTGTTATGGATTACTCTTGGACTAAAGATCGTAACCCTAAACTTCAACAATTACTTCAACTTGCCAAGAGAATCAACCAAGGTTTTTCGTTGCTGAATTATCTATATGAATCCTATATATATTATTTTATCAGAATTTGAAACAAAAGTTTGTTTTTGCAGATCCAAATTCAAAAGCACCAATGTCATACATAATGCTACACCCAGCATCGGGACAATGTGTTAAGTCCAATAGCAACGGAGAGGCTGAACTTGGTGATTGTACAGCTCTGACCCAATGGAACCATAGTGGAGATGCAAGTTTGATGAAGTTGTCAAGCGGGAAATGTTTGAAGTCTGCTGGAGATGGAAAGTCGCCGGTGGTATCCGATGATTGCTCTGGTGATGGAAGCAATTGGACGGTGGCTTCAAAAGCAAAGCTTCAATTGGCCACCAAAATTGGTGAGGACAACTTTTGTTTGGAAAAGGAATCAGACACTTCAATTGTTGTGAAGAAATGCATTTGCCTAGACGAGGGGAGTTGCATGGATGATCCTCAAAGCCAATGGTTCAAGCTTATTCCAACCAATGTTGCTTAG

mRNA sequence

ATGGTTGCTCAAGGACTTGATAAGCGTCCATTGAAAGAGTTGGTAGCTGAGATTAGTACTCGCAAATTCAATTGTGTTCGATTGACGTGGTCTGTCCATATGTTTACTCGTTATGCATATGAGACTATTGGTGATGTCTTTGACGGTCTAGATATTGCTGACGTAAAAAATGGAGTTAAAAAGCACAATCCTGAAATATTGAAAATGACGGTTGCTAAGGCGTTTCAAACTGTCATTAATGCTTTGGGCTCGGAGAAAATTATGGTTATTTTAGATAATCATATTAGCCAACCAAGATGGTGTTGCTCTCTCGACGATGGAAATGGGTTCTTTGGAGATTGCAATTTTAACCCTAGTGAATGGCTTCAAAGCTTGTCATTTGTTGCCGTGCAATTCACATGCAATCCATATGTGGTAGGAATGAGTTTAAGAAACGAGCTTCGAGGGTCATTATCAAATGCTGATGCCTGGTGCTATTATGTGAGACAAGGAAGCCAACTGATCCATAGAATAAACCCTAATCTCCTTGTAGTCATTTCTGGACTAAACTATGACAATGATCTAAGCCATCTAAAGAAAAGACCTCTAGGCTACACTTTACACAACAAAGTAGTGTTGGAGGCACATCTCTACTCGTTCAGTGGAGATCATGAAAGCAAGTTCACAAAGAATCCATTAAATGTAATATGCGACCAAATCATGGAGAAGTTTGAAAAGGAAGCTGGGTTTGTGGTGGACATGGAGAATCCATATCCTTTGTTCCTTAGTGAATTCGGGTACGATCAATCGGGTGGAAATGATGCTCAAAATCGATTCATGAGCTGCTTTTTAGCTCGAATTGTAGAGAAGGATATTGATTGGGCGTTGTGGGCTTTCCAAGGAAGTTATATGTATAGACAAGGACATCAAGATATTGAAGAAAGTTTTGGTGTTATGGATTACTCTTGGACTAAAGATCGTAACCCTAAACTTCAACAATTACTTCAACTTGCCAAGAGAATCAACCAAGATCCAAATTCAAAAGCACCAATGTCATACATAATGCTACACCCAGCATCGGGACAATGTGTTAAGTCCAATAGCAACGGAGAGGCTGAACTTGGTGATTGTACAGCTCTGACCCAATGGAACCATAGTGGAGATGCAAGTTTGATGAAGTTGTCAAGCGGGAAATGTTTGAAGTCTGCTGGAGATGGAAAGTCGCCGGTGGTATCCGATGATTGCTCTGGTGATGGAAGCAATTGGACGGTGGCTTCAAAAGCAAAGCTTCAATTGGCCACCAAAATTGGTGAGGACAACTTTTGTTTGGAAAAGGAATCAGACACTTCAATTGTTGTGAAGAAATGCATTTGCCTAGACGAGGGGAGTTGCATGGATGATCCTCAAAGCCAATGGTTCAAGCTTATTCCAACCAATGTTGCTTAG

Coding sequence (CDS)

ATGGTTGCTCAAGGACTTGATAAGCGTCCATTGAAAGAGTTGGTAGCTGAGATTAGTACTCGCAAATTCAATTGTGTTCGATTGACGTGGTCTGTCCATATGTTTACTCGTTATGCATATGAGACTATTGGTGATGTCTTTGACGGTCTAGATATTGCTGACGTAAAAAATGGAGTTAAAAAGCACAATCCTGAAATATTGAAAATGACGGTTGCTAAGGCGTTTCAAACTGTCATTAATGCTTTGGGCTCGGAGAAAATTATGGTTATTTTAGATAATCATATTAGCCAACCAAGATGGTGTTGCTCTCTCGACGATGGAAATGGGTTCTTTGGAGATTGCAATTTTAACCCTAGTGAATGGCTTCAAAGCTTGTCATTTGTTGCCGTGCAATTCACATGCAATCCATATGTGGTAGGAATGAGTTTAAGAAACGAGCTTCGAGGGTCATTATCAAATGCTGATGCCTGGTGCTATTATGTGAGACAAGGAAGCCAACTGATCCATAGAATAAACCCTAATCTCCTTGTAGTCATTTCTGGACTAAACTATGACAATGATCTAAGCCATCTAAAGAAAAGACCTCTAGGCTACACTTTACACAACAAAGTAGTGTTGGAGGCACATCTCTACTCGTTCAGTGGAGATCATGAAAGCAAGTTCACAAAGAATCCATTAAATGTAATATGCGACCAAATCATGGAGAAGTTTGAAAAGGAAGCTGGGTTTGTGGTGGACATGGAGAATCCATATCCTTTGTTCCTTAGTGAATTCGGGTACGATCAATCGGGTGGAAATGATGCTCAAAATCGATTCATGAGCTGCTTTTTAGCTCGAATTGTAGAGAAGGATATTGATTGGGCGTTGTGGGCTTTCCAAGGAAGTTATATGTATAGACAAGGACATCAAGATATTGAAGAAAGTTTTGGTGTTATGGATTACTCTTGGACTAAAGATCGTAACCCTAAACTTCAACAATTACTTCAACTTGCCAAGAGAATCAACCAAGATCCAAATTCAAAAGCACCAATGTCATACATAATGCTACACCCAGCATCGGGACAATGTGTTAAGTCCAATAGCAACGGAGAGGCTGAACTTGGTGATTGTACAGCTCTGACCCAATGGAACCATAGTGGAGATGCAAGTTTGATGAAGTTGTCAAGCGGGAAATGTTTGAAGTCTGCTGGAGATGGAAAGTCGCCGGTGGTATCCGATGATTGCTCTGGTGATGGAAGCAATTGGACGGTGGCTTCAAAAGCAAAGCTTCAATTGGCCACCAAAATTGGTGAGGACAACTTTTGTTTGGAAAAGGAATCAGACACTTCAATTGTTGTGAAGAAATGCATTTGCCTAGACGAGGGGAGTTGCATGGATGATCCTCAAAGCCAATGGTTCAAGCTTATTCCAACCAATGTTGCTTAG

Protein sequence

MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVKKHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSEWLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVISGLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKEAGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQGHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSNSNGEAELGDCTALTQWNHSGDASLMKLSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVASKAKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA

Homology

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BLAST of CmoCh12G006320 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 6.1e-101
Identity = 187/488 (38.32%), Postives = 283/488 (57.99%), Query Frame = 0


Query: 4   QGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAY--ETIGDVFDGLDIADVKNGVKK 63
           +GL++ P+  +   IS+  FNCVRLT+S+HM TR +Y   T+   F  L++ +  +G++ 
Sbjct: 64  EGLNRLPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEH 123

Query: 64  HNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSEW 123
           +NPE+L +    A+  V+ AL    +MVILDNH+S+P+WCC++DDGNGFFGD  FNP+ W
Sbjct: 124 NNPELLDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTW 183

Query: 124 LQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVISG 183
           ++ L  +A  F   P VV MSLRNELRG+ S   +W  +++ G+  +H+ NP +LV++SG
Sbjct: 184 VEGLGLMATYFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSG 243

Query: 184 LNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKEA 243
           L +D DLS L   P+      K+V E H YSF     +       N +C     +F+   
Sbjct: 244 LQFDTDLSFLPVLPVTLPFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNV 303

Query: 244 GFVVDMEN--PYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYR 303
           GFV    N    PLF+SEFG DQ   ND  NR+++C LA + E+D+DWALW   GSY YR
Sbjct: 304 GFVTSSANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYR 363

Query: 304 QGHQ---DIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPN-SKAPMSYIMLHPASGQ 363
              Q   D EE++G  ++ W++ RNP     L+  ++  QDP  +  P   I+ HPASG 
Sbjct: 364 SDKQPVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGL 423

Query: 364 CVKSNSNGEAELGDCTAL-TQWNHSGDAS--LMKLSSGKCLKSAGDGKSPVVSDDCSGDG 423
           CV+S       LG C ++ ++WN+       +  + S  C+ + G+G   +++++CS   
Sbjct: 424 CVESGIGNTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPN 483

Query: 424 SN-WTVASKAKLQLATKI-GEDN----FCLEKESDTSIVVKKCICLDEGSCMD--DPQSQ 473
           +  W+  S A+LQL T++ G+D      CL+      I   +CIC+ +  C    +P+ Q
Sbjct: 484 NTLWSTVSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICITDSHCYPKLNPEKQ 543

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BLAST of CmoCh12G006320 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 8.5e-10
Identity = 75/347 (21.61%), Postives = 134/347 (38.62%), Query Frame = 0


Query: 2   VAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVKK 61
           V  GL  R  K+++ ++    FN VRL +          +T+    D             
Sbjct: 58  VMHGLWARNWKDMIVQMQGLGFNAVRLPFCP---ATLRSDTMPASID-----------YS 117

Query: 62  HNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSEW 121
            N ++  +T  +    VI    +  + V+LD+H      C  + +    +   ++  ++W
Sbjct: 118 RNADLQGLTSLQILDKVIAEFNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQW 177

Query: 122 LQSLSFVAVQFTCNPYVVGMSLRNELRGSL-----SNADAWCYYVRQGSQLIHRINPNLL 181
           L  L FVA ++   PYV+G+ L+NE  G+      + A  W     +GS  +  + P  L
Sbjct: 178 LADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWL 237

Query: 182 VVISGLN------------YDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKN 241
           + + G+             +  +L  L   PL     N+++L  H+Y      +S F  +
Sbjct: 238 IAVEGITDNPVCSTNGGIFWGGNLQPLACTPLNIPA-NRLLLAPHVYGPDVFVQSYFNDS 297

Query: 242 PLNVICDQIMEK-FEKEAGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEK 301
                   I E+ F + AG        + L L EFG     G+     +    +  +  K
Sbjct: 298 NFPNNMPAIWERHFGQFAG-------THALLLGEFGGKYGEGDARDKTWQDALVKYLRSK 357

Query: 302 DIDWALWAFQGSYMYRQGHQDIEESFGVMDYSWTKDRNPKLQQLLQL 331
            I+      QG   Y   + +  ++ G++   WT  R  K+  L  L
Sbjct: 358 GIN------QG--FYWSWNPNSGDTGGILRDDWTSVRQDKMTLLRTL 368

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BLAST of CmoCh12G006320 vs. ExPASy TrEMBL
Match: A0A6J1GKH5 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111454747 PE=3 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.1e-285
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK 60
           MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK
Sbjct: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK 60

Query: 61  KHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE 120
           KHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE
Sbjct: 61  KHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE 120

Query: 121 WLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVIS 180
           WLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVIS
Sbjct: 121 WLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVIS 180

Query: 181 GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE 240
           GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE
Sbjct: 181 GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE 240

Query: 241 AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ 300
           AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ 300

Query: 301 GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSN 360
           GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSN
Sbjct: 301 GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSN 360

Query: 361 SNGEAELGDCTALTQWNHSGDASLMKLSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVASK 420
           SNGEAELGDCTALTQWNHSGDASLMKLSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVASK
Sbjct: 361 SNGEAELGDCTALTQWNHSGDASLMKLSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVASK 420

Query: 421 AKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA 475
           AKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA
Sbjct: 421 AKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA 474

[Close]

BLAST of CmoCh12G006320 vs. ExPASy TrEMBL
Match: A0A6J1KLZ2 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496466 PE=3 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.2e-280
Identity = 464/474 (97.89%), Postives = 469/474 (98.95%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK 60
           MVAQGLDKRPLKELVAEIS+RKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK
Sbjct: 1   MVAQGLDKRPLKELVAEISSRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK 60

Query: 61  KHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE 120
           +HNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE
Sbjct: 61  RHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE 120

Query: 121 WLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVIS 180
           WLQ LSFVAVQFTCNPYV+GMSLRNELRG LSNADAWCYYVRQGSQLIHRINPNLLVVIS
Sbjct: 121 WLQGLSFVAVQFTCNPYVIGMSLRNELRGPLSNADAWCYYVRQGSQLIHRINPNLLVVIS 180

Query: 181 GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE 240
           GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE
Sbjct: 181 GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE 240

Query: 241 AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ 300
           AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ 300

Query: 301 GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSN 360
           GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSK PMSYIMLHPASGQCVKSN
Sbjct: 301 GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKEPMSYIMLHPASGQCVKSN 360

Query: 361 SNGEAELGDCTALTQWNHSGDASLMKLSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVASK 420
           SNGEAELGDCTA TQWNH+GDASLMK SSGKCLKSAGDGKSPVVSDDCSGDGSNWTVASK
Sbjct: 361 SNGEAELGDCTAPTQWNHTGDASLMKSSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVASK 420

Query: 421 AKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA 475
           AKLQLATKIGEDNFCLEKESDTSIVVKKCICLD+GSCMDDPQSQWFKLIPTNVA
Sbjct: 421 AKLQLATKIGEDNFCLEKESDTSIVVKKCICLDQGSCMDDPQSQWFKLIPTNVA 474

[Close]

BLAST of CmoCh12G006320 vs. ExPASy TrEMBL
Match: A0A6J1JQD4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=3 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 2.0e-251
Identity = 411/475 (86.53%), Postives = 445/475 (93.68%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK 60
           MVAQGLDKRPLKEL AEIS+R+FNCVRLTWS+HMFTRYAYETIGDV DGLDI DVKNGV+
Sbjct: 1   MVAQGLDKRPLKELAAEISSRQFNCVRLTWSIHMFTRYAYETIGDVLDGLDIGDVKNGVE 60

Query: 61  KHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE 120
           KHNPEILKM+V KAFQTVIN LGSE IMVILDNHISQPRWCCSLDDGNGFFGD NFNP+E
Sbjct: 61  KHNPEILKMSVVKAFQTVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 121 WLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVIS 180
           WLQ LSFVA QFTCNP+V+GMSLRNELRG  +N DAWCYY+RQGSQLIHRINPN+LV+IS
Sbjct: 121 WLQGLSFVATQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILVIIS 180

Query: 181 GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE 240
           GLNYDNDLS+LKKRPLGYTLHNKVVLEAHLYSFSGD+ESKF K PLN+IC++IMEKFE+E
Sbjct: 181 GLNYDNDLSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 241 AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ 300
           AGFVVDMENPYPLFLSEFGYDQSGGN+A+NRFMSCFLARI EKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMENPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 301 GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSN 360
           GH D +ESFGVMD+SWTKDRNPKL QLLQL KRINQDPNSKAPMSYI+LH  SGQCVKS+
Sbjct: 301 GHTDPDESFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIVLHSVSGQCVKSD 360

Query: 361 SNGEAELGDCTALTQWNHSGDASLMK-LSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVAS 420
           + G  ELGDCTA TQWNH+GDAS MK LS+G+CLKSAGDGKSPVVS +CSGDGSNWTV+S
Sbjct: 361 NKGGIELGDCTAPTQWNHTGDASPMKLLSNGQCLKSAGDGKSPVVSAECSGDGSNWTVSS 420

Query: 421 KAKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA 475
           KAKLQLATKIGEDNFCLEKESD SIV+KKCICLDE SCMDDPQ+QWFKL+PTNV+
Sbjct: 421 KAKLQLATKIGEDNFCLEKESDASIVLKKCICLDEESCMDDPQTQWFKLVPTNVS 475

[Close]

BLAST of CmoCh12G006320 vs. ExPASy TrEMBL
Match: A0A6J1GQA8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111456519 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.2e-248
Identity = 405/475 (85.26%), Postives = 444/475 (93.47%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK 60
           MVAQGLDKRPLKEL AEIS+R+FNCVRLTWSVHMFTRYAYETIGDV DGLDI+DVKNGV+
Sbjct: 1   MVAQGLDKRPLKELAAEISSRQFNCVRLTWSVHMFTRYAYETIGDVLDGLDISDVKNGVE 60

Query: 61  KHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE 120
           KHN E+LKM+VAKAFQTVIN LGSE IMVILDNHISQ RWCCSLDDGNGFFGD NFNP+E
Sbjct: 61  KHNSEVLKMSVAKAFQTVINCLGSEGIMVILDNHISQSRWCCSLDDGNGFFGDRNFNPNE 120

Query: 121 WLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVIS 180
           W Q LSFVA QFTCNP+V+GMSLRNELRG  +N DAWCYY+RQGSQLIHRINPN+L++IS
Sbjct: 121 WFQGLSFVATQFTCNPHVIGMSLRNELRGPFTNNDAWCYYMRQGSQLIHRINPNILIIIS 180

Query: 181 GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE 240
           GLNYDNDLS+LKKRPLGYTLHNKVVLEAHLYSFSGD+ESKF K PLN+IC++IMEKFE+E
Sbjct: 181 GLNYDNDLSYLKKRPLGYTLHNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 241 AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ 300
           AGFVVDMENPYPLFLSEFGYDQSGGN+A+NRFMSCFLARI EKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMENPYPLFLSEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 301 GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSN 360
           GH D +ESFGVMD+SWTKDRNPKL QLLQL KRINQDPNSKAPMSYIMLH  SGQCVKS+
Sbjct: 301 GHTDPDESFGVMDHSWTKDRNPKLAQLLQLVKRINQDPNSKAPMSYIMLHSVSGQCVKSD 360

Query: 361 SNGEAELGDCTALTQWNHSGDASLMK-LSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVAS 420
           + G  ELGDCT+ TQWNH+GDAS MK LS+G+CLKSAGDG+SPVVS +CSGDGSNWTV+S
Sbjct: 361 NKGGIELGDCTSPTQWNHTGDASPMKLLSNGQCLKSAGDGQSPVVSAECSGDGSNWTVSS 420

Query: 421 KAKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA 475
           KAKLQLATK+GEDNFCLEK+SD SIV+KKCICLDE SCMDDPQ+QWFKL+PTNV+
Sbjct: 421 KAKLQLATKVGEDNFCLEKKSDASIVLKKCICLDEESCMDDPQTQWFKLVPTNVS 475

[Close]

BLAST of CmoCh12G006320 vs. ExPASy TrEMBL
Match: A0A6J1GZR4 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459103 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.1e-245
Identity = 400/475 (84.21%), Postives = 438/475 (92.21%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYETIGDVFDGLDIADVKNGVK 60
           MVAQGLDKRPLKEL +EI++R+FNCVRLTWSVHMFTRYAYE +GDV DGLDIADVK+GV 
Sbjct: 1   MVAQGLDKRPLKELASEITSRQFNCVRLTWSVHMFTRYAYECVGDVLDGLDIADVKSGVA 60

Query: 61  KHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNPSE 120
           KHNPEILKMT+AKAFQ VIN LGSE IMVILDNHISQPRWCCSLDDGNGFFGD NFNP+E
Sbjct: 61  KHNPEILKMTIAKAFQMVINCLGSEGIMVILDNHISQPRWCCSLDDGNGFFGDRNFNPNE 120

Query: 121 WLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVVIS 180
           WLQ LSFVA QFTCNP+VVGMSLRNELRG  +N DAWCYY+R+GSQLIHRINPNLL++IS
Sbjct: 121 WLQGLSFVAAQFTCNPHVVGMSLRNELRGPFTNNDAWCYYMRRGSQLIHRINPNLLIIIS 180

Query: 181 GLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFEKE 240
           GLNYDNDLS LKKRPLGYT  NKVVLEAHLYSFSGD+ESKF K PLN+IC++IMEKFE+E
Sbjct: 181 GLNYDNDLSQLKKRPLGYTFQNKVVLEAHLYSFSGDNESKFVKKPLNIICNEIMEKFERE 240

Query: 241 AGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMYRQ 300
           AGFVVDM+NPYPLF+SEFGYDQSGGN+A+NRFMSCFLARI EKDIDWALWAFQGSYMYRQ
Sbjct: 241 AGFVVDMKNPYPLFISEFGYDQSGGNEAENRFMSCFLARIAEKDIDWALWAFQGSYMYRQ 300

Query: 301 GHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVKSN 360
           GH+D +ESFGVMD+SWTKDRNPKL+Q+LQL KR+NQD NSKAPMSYI+LHP SGQCVKS+
Sbjct: 301 GHEDPDESFGVMDHSWTKDRNPKLKQMLQLIKRVNQDLNSKAPMSYIILHPVSGQCVKSD 360

Query: 361 SNGEAELGDCTALTQWNHSGDASLMK-LSSGKCLKSAGDGKSPVVSDDCSGDGSNWTVAS 420
             G  ELGDC   TQWNHSGDAS MK LS+G+CLKSAGDGKSP+VS DCSGD  NWTVAS
Sbjct: 361 GKGGIELGDCATPTQWNHSGDASPMKLLSNGQCLKSAGDGKSPIVSTDCSGDEYNWTVAS 420

Query: 421 KAKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKLIPTNVA 475
           KAKLQL+TK+GED+FCLEKESDTSIVVKKCICLD  SCMDDPQSQWFKL+PTNVA
Sbjct: 421 KAKLQLSTKVGEDSFCLEKESDTSIVVKKCICLDAESCMDDPQSQWFKLVPTNVA 475

[Close]

BLAST of CmoCh12G006320 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 346.7 bits (888), Expect = 3.0e-95
Identity = 192/477 (40.25%), Postives = 276/477 (57.86%), Query Frame = 0


Query: 2   VAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTR---YAYETIGDVFDGLDIADVKNG 61
           VA+GL K+PL  +  +I +  FNCVRLTW +++ T     A+ T+        + +  +G
Sbjct: 55  VAEGLSKQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSG 114

Query: 62  VKKHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNP 121
            + HNP IL + + KAFQ V+  L   ++MVILDNHISQP WCCS +DGNGFFGD + NP
Sbjct: 115 FQTHNPTILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNP 174

Query: 122 SEWLQSLSFVAVQF-TCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLV 181
             W++ L  +A  F   +  VVGMSLRNELRG   N   W  Y+R+G++ +H +NPN+LV
Sbjct: 175 QVWIKGLKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLV 234

Query: 182 VISGLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKF 241
           ++SGLNY  DLS L++RP   +   KVV E H Y F    E     + LN IC +  EK 
Sbjct: 235 IVSGLNYATDLSFLRERPFEVSFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKM 294

Query: 242 EKEAGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYM 301
            K +GF+  +E   PLF+SEFG DQ G N   N+F+SCF+A   ++D+DW+LW   GSY 
Sbjct: 295 MKMSGFL--LEKGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYY 354

Query: 302 YRQGHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCV 361
            R+     +ES+GV+D++W+  RN  + Q++   +          P   IM HP++G C+
Sbjct: 355 IREKSIGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK-IMFHPSTGLCI 414

Query: 362 KSNSNGEAELGDCTALTQWNHSGDASL-MKLSSGKCLKSAGDGKSPVVSDDCSGD-GSNW 421
              S  + +LG C     W  S    L +      CLK+   GKS  +    S    S W
Sbjct: 415 VRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKW 474

Query: 422 TVASKAKLQLATKIGEDNF--CLEKESD-TSIVVKKCICLDEGSCMDDPQSQWFKLI 470
            + S +K+QL++ I ++ F  CL+ +++  +IV   C CL  G+   DP+SQWFKL+
Sbjct: 475 KLFSDSKMQLSS-ITKNGFSVCLDVDTENNNIVTNSCKCL-RGNSSCDPRSQWFKLV 522

[Close]

BLAST of CmoCh12G006320 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 338.2 bits (866), Expect = 1.1e-92
Identity = 172/478 (35.98%), Postives = 269/478 (56.28%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYE---TIGDVFDGLDIADVKN 60
           +VA+GL K+ + +L  +I    FNCVR TW + + T        T+   F  L + D  +
Sbjct: 34  VVAEGLSKQSVDDLAKKIMAMGFNCVRFTWPLDLATNETLANNVTVRQSFQSLGLNDDIS 93

Query: 61  GVKKHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFN 120
           G +  NP ++ + + +A++ V+  LG+  +MVILDNH+++P WCC  +DGNGFFGD  F+
Sbjct: 94  GFETKNPSMIDLPLIEAYKKVVAKLGNNNVMVILDNHVTKPGWCCGYNDGNGFFGDTFFD 153

Query: 121 PSEWLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLV 180
           P+ W+  L+ +A+ F     VVGMSLRNELRG   N D W  Y++QG++ +H  NPN+LV
Sbjct: 154 PTTWIAGLTKIAMTFKGATNVVGMSLRNELRGPKQNVDDWFKYMQQGAEAVHEANPNVLV 213

Query: 181 VISGLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKF 240
           ++SGL+YD DLS ++ R +  T   K+V E H YSF+  + +  +KNP N  C +I++  
Sbjct: 214 ILSGLSYDTDLSFVRSRHVNLTFTRKLVFELHRYSFTNTN-TWSSKNP-NEACGEILKSI 273

Query: 241 EKEAGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYM 300
           E   GF +     +P+FLSEFG D  G N   NR++ C L    E D+DW++W  QGSY 
Sbjct: 274 ENGGGFNL---RDFPVFLSEFGIDLRGKNVNDNRYIGCILGWAAENDVDWSIWTLQGSYY 333

Query: 301 YRQGHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCV 360
            R+G   + E +G++D  W + R+    Q L L     Q P S++ +  ++ HP +G C+
Sbjct: 334 LREGVVGMSEFYGILDSDWVRVRSQSFLQRLSLILSPLQGPGSQSKVYNLVFHPLTGLCM 393

Query: 361 KSN--SNGEAELGDCTALTQWNHSGDASLMKLSSGKCLKSAGDGKSPVVSD-DCSGDG-S 420
             +     +  LG C     W+++   +L       CL+S G      +S+  CS    S
Sbjct: 394 LQSILDPTKVTLGLCNESQPWSYTPQNTLTLKDKSLCLESTGPNAPVKLSETSCSSPNLS 453

Query: 421 NWTVASKAKLQLATKIGEDNFCLEKESDTSIVVKKCICL--DEGSCMDDPQSQWFKLI 470
            W   S + + LA K   ++ CL+ +   +++   C C+  ++ SC  DP SQWFK++
Sbjct: 454 EWETISASNMLLAAKSTNNSLCLDVDETNNLMASNCKCVKGEDSSC--DPISQWFKIV 504

[Close]

BLAST of CmoCh12G006320 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.8e-92
Identity = 179/478 (37.45%), Postives = 269/478 (56.28%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYE---TIGDVFDGLDIADVKN 60
           +VA+GL K+P+  +  +I    FNCVRLTW + + T        T+   F  L + D   
Sbjct: 64  VVAEGLSKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIV 123

Query: 61  GVKKHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFN 120
           G + +NP I+ + + +A++TV+  LG+  +MVILDNH+++P WCC+ DDGNGFFGD  F+
Sbjct: 124 GFQTNNPSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFD 183

Query: 121 PSEWLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLV 180
           P+ W+ +L  +A  F     VVGMSLRNELRG   N + W  Y++QG++ +H  N  +LV
Sbjct: 184 PTVWVAALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLV 243

Query: 181 VISGLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKF 240
           ++SGL++D DLS ++ RP+  +   K+V E H YSFS D  S    NP N IC +++ + 
Sbjct: 244 ILSGLSFDADLSFVRSRPVKLSFTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNRI 303

Query: 241 EKEAGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYM 300
               G++  +   +PLFLSEFG D+ G N   NR+  C      E D+DW+LWA  GSY 
Sbjct: 304 GNGGGYL--LNQGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYY 363

Query: 301 YRQGHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCV 360
            RQG   + E +GV+D  W   RN    Q +   +   Q P  +     ++ HP +G C+
Sbjct: 364 LRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCI 423

Query: 361 KSNSNGE--AELGDCTALTQWNHSGDASLMKLSSGKCLKSAGDGKSPV--VSDDCSGDGS 420
             + +      LG C +   W+++  A  +K     CL+S G  K+PV      CS  GS
Sbjct: 424 VRSLDDPKMLTLGPCNSSEPWSYTKKALRIK-DQQLCLQSNGP-KNPVTMTRTSCSTSGS 483

Query: 421 NWTVASKAKLQLA-TKIGEDNFCLEKESDTSIVVKKCICLD-EGSCMDDPQSQWFKLI 470
            W   S +++ LA T   + + CL+ ++  ++V   C CL  + SC  +P SQWFK+I
Sbjct: 484 KWQTISASRMHLASTTSNKTSLCLDVDTANNVVANACKCLSKDKSC--EPMSQWFKII 533

[Close]

BLAST of CmoCh12G006320 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 4.0e-87
Identity = 179/476 (37.61%), Postives = 259/476 (54.41%), Query Frame = 0


Query: 1   MVAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMF---TRYAYETIGDVFDGLDIADVKN 60
           +VA+GL  +P+  +  +I    FNCVRLTW + +    T     T+   F+   +     
Sbjct: 57  VVAEGLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQ 116

Query: 61  GVKKHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFN 120
           G+  HNP I+   +   FQ V+ +LG   +MVILDNH + P WCCS DD + FFGD  FN
Sbjct: 117 GIYTHNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFN 176

Query: 121 PSEWLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLV 180
           P  W+  L  +A  F     VVGMSLRNELRG    +  W  Y+++G++ +H  NPN+LV
Sbjct: 177 PDLWMLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLV 236

Query: 181 VISGLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKF 240
           ++SGLN+D DLS LK RP+  +   K+VLE H YSF+ D   ++  + +N  C Q+  K 
Sbjct: 237 ILSGLNFDADLSFLKDRPVNLSFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKE 296

Query: 241 EKEAGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYM 300
            +  GFV+D    +PLFLSEFG DQ GG+   NR+M+C LA   EKD+DWA+WA  G Y 
Sbjct: 297 RRTGGFVLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYY 356

Query: 301 YRQGHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCV 360
           +R+G + + E++G++D +W    N    + L + +  +  P  K      + HP +G C+
Sbjct: 357 FREGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCL 416

Query: 361 KSNSN---GEAELGDCTALTQWNHS-GDASLMKLSSGKCLK-SAGDGKSPVVSDDCSGDG 420
              S+    E  LG CT    W++S G    ++     CL+     GKS  +   C+   
Sbjct: 417 VRKSHCHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICT--K 476

Query: 421 SNWTVASKAKLQLATKIGEDNFCLEKESDTSIVVKKCICLDEGSCMDDPQSQWFKL 469
                A+K  L   T  G    CL+ +SD ++V   C CL  G    +P SQWFK+
Sbjct: 477 IEQISATKMHLSFNTSDG-SLVCLDVDSDNNVVANSCNCL-TGDTTCEPASQWFKI 525

[Close]

BLAST of CmoCh12G006320 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-72
Identity = 164/479 (34.24%), Postives = 247/479 (51.57%), Query Frame = 0


Query: 2   VAQGLDKRPLKELVAEISTRKFNCVRLTWSVHMFTRYAYE---TIGDVFDGLDIADVKNG 61
           VA+GL K+PL  +  +I +  FNCVRLTW + + T        T+   F+ L + +   G
Sbjct: 56  VAEGLSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLG 115

Query: 62  VKKHNPEILKMTVAKAFQTVINALGSEKIMVILDNHISQPRWCCSLDDGNGFFGDCNFNP 121
           ++ HNP++L + +  AFQ V++ LG   +MVILDNH++ P WCC  +D + FFG  +F+P
Sbjct: 116 IQTHNPKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDP 175

Query: 122 SEWLQSLSFVAVQFTCNPYVVGMSLRNELRGSLSNADAWCYYVRQGSQLIHRINPNLLVV 181
             W + L  +A  F    +V+GMSLRNE RG+    D W  ++ QG++ +H  NP LLV+
Sbjct: 176 LVWAKGLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVI 235

Query: 182 ISGLNYDNDLSHLKKRPLGYTLHNKVVLEAHLYSFSGDHESKFTKNPLNVICDQIMEKFE 241
           +SG+++D +LS L+ R +  +  +K+V E H YSFS D    + K+  N  C +I+EK  
Sbjct: 236 LSGIDFDTNLSFLRDRSVNVSFTDKLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVT 295

Query: 242 KEAGFVVDMENPYPLFLSEFGYDQSGGNDAQNRFMSCFLARIVEKDIDWALWAFQGSYMY 301
              GF+  +   +PL LSEFG DQ GG+ + NR+M+C +A   E D+DWA+WA  G Y  
Sbjct: 296 HNGGFL--LGRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYL 355

Query: 302 RQGHQDIEESFGVMDYSWTKDRNPKLQQLLQLAKRINQDPNSKAPMSYIMLHPASGQCVK 361
           R G                    P L+                 P   ++ HP++G CV 
Sbjct: 356 RTG--------------------PGLR-----------------PNKNLLFHPSTGLCVT 415

Query: 362 SNSNGE---AELGDCTALTQWNHSGDASLMKLSSGKCLKS---AGDGKSPVVSDDCSGDG 421
           +N +       LG C     W  +    ++ ++   C+++    G      V   CS  G
Sbjct: 416 NNPSDNIPTLRLGPCPKSDPWTFNPSEGILWINK-MCVEAPNVVGQKVKLGVGTKCSKLG 475

Query: 422 SNWTVASKAKLQLATKIGEDNFCLE-KESDTSIVVKKCICLD-EGSCMDDPQSQWFKLI 470
                A+K  L   T  G    CL+  E D S+V  +C  L  + SC  DP SQWFK++
Sbjct: 476 Q--ISATKMHLSFKTSNGL-LLCLDVDERDNSVVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:

BLAST of CmoCh12G006320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	6.1e-101	38.32	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P19487	8.5e-10	21.61	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of CmoCh12G006320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GKH5	2.1e-285	100.00	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111454747 P...	[more]
A0A6J1KLZ2	1.2e-280	97.89	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496466 PE=...	[more]
A0A6J1JQD4	2.0e-251	86.53	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111487386 PE=...	[more]
A0A6J1GQA8	1.2e-248	85.26	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111456519 P...	[more]
A0A6J1GZR4	2.1e-245	84.21	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459103 P...	[more]

BLAST of CmoCh12G006320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26130.1	3.0e-95	40.25	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	1.1e-92	35.98	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT1G13130.1	1.8e-92	37.45	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	4.0e-87	37.61	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	1.2e-72	34.24	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.80.10.50		coord: 344..466 e-value: 1.0E-6 score: 30.9
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..331 e-value: 4.7E-57 score: 195.7
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 1..470
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 10..292 e-value: 8.1E-22 score: 78.0
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 4..331
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 343..467

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G006320.1	CmoCh12G006320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds