CmoCh12G000650 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh12G000650
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAcyl-[acyl-carrier-protein] desaturase
LocationCmo_Chr12: 347481 .. 351949 (+)
RNA-Seq ExpressionCmoCh12G000650
SyntenyCmoCh12G000650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGCAGCTTGCGAGTTTCAGATCTCCTAAGTTCGTTGTGGCCTCCACTCTCCGCTCCACTTCCAGGTAGATTCTCCTTCTTTTTTCTTATTCTGTTTTTCAAATCTTTGTGTATTGGTTTTGATCTCTTTTATTATAATGGAGTGTGTTCCTGAATGGCGATGAATATGAAGCCGTTTCAATTGCATTTATATCGTTCTGTTTTTCTTCGTTTCCTATCGCATATCACGTCGACTGAGTGATTATGGCTCATTCCTGCCGGTACGAATAGGTTTTTTCCTTATTCTGTTATAATTCCAGAAAATGAAAATAGATGTTCAATTTTCTTTCCTGTGGCCGTAAATTTGATCTGTAGTTAATCGTGTGGTTGCATGATTGTTCATTTGGTATTCTTTGGTGTATTTGGCGGAGCGGTGTTCACTTTTTCTGTCTTCTTGCGAGCGTTCTATCTTGATCATTTATTCTTCGCTCTGATCTTCCGCAGCTCATATATATATTCCTTTCTCCTACATTTCTCTGCTTATATGTTCATAAACCGATAATAGACCAAGTTGTTCAGGGTAGAATTCTCTGTAATCCGATTCCTTTCTTGTTCTACCTTTAGACAGTATTTTTAATTGTAAACAGCTCGTTTGCAATGAGGACTTAATTATTTAGAAGAATTGTAATCTGCCTATGCAGGAACGAGAAATCTCTGTTTGCTTAACTATTTTGCCTTGTTTTGTGTATTTCGTAAACATACCAGGGAGGTCGAGGCCCTTAAGAAACCCTTTATACCTCCTAGGGAAGTGCATCCTCAAGTAACTCACTCAATGCCACCACAGAAGATAGAGATGTTTAAATGTCTGGAGGATTGGGCAGCAGAGAACATTTTAGTTTATTTGAAACCAGTGGAGAGGTGTTGGCAACCACTGGATTTGTTGCCAGATCCGGCATCTGAAGGATTTCAGGAGCAAGTGATGGAGCTTAGAGAGAGGGCAAAGGAAGTCCCTGATGATTACTTCATCGTTTTAGTTGGAGACATGATTACAGAAGATGCCCTTCCTACTTACCAGACAATGCTTAATACCATGGATGGAGTTAGGGATGAAACTGGAGCAAGCCTCAGTTCTTGGGCCATTTGGACGAGGTCTTGGACTGCTGAAGAGAACAGGCATGGTGACGTACTAAACAAATATCTTTACCTATCAGGGCGAGTTGATATGAGGCAGGTTGAGAAAACTATTCAGTATTTGATTCGATCAGGAATGGTGAGATCCATCTACCACTATCCAATTTTCCTTTACCTCATCACATTACTTTCACAATTTGCTTTTCTTCAAACCCATAGTTATTGAGAGGACAGTATGCACATTAAGACACACGAAAGCGCACACACTTCCATACTATGCTATACTTAATTCATTACAACCTTCACAACTGCTCAAGCATTTTGGAGTATTCCACGCATTTTGCTGGATATTGACACAATTTCTCCATTTTTTCACTAGTCCTGCTAAATTTTTTATTAAAATTTCATATGGATTGTGTCTATTTAGGACCCAGGAACTGAAAACAACCCTTACCTAGGATTTATATACACCTCATTTCAAGAGAGAGCAACCTTCATCTCCCATGGGAACACTGCCAGATTAGCAAAGGAGCACGGAGACATAAAGTTGGCCCAATTATGTGGAACAATTGCTGCAGATGAGAAGCGACACGAAGCTGCATATACAAAAATAGTTGAAAAGCTGTTTGAGATAGACCCTGAAGGAACCATCCTGTGTTTTGAAGACATGATGAGGAAGAACATTACAATGCCAGCCCACATGATGTACGATGGCCGTGACAACAACCTCTTCAATCACTTCTCGGCACTTGCTCAGAGGCTGGGGGTTTATACAGCCAAGGACTACGCCGACATATTGGAGTATTTGGTTGGGAGATGGAAGGTGGAACAGCTCAAAGGACTGTCAGGTCACGGGCAGAAGGCTCAGGATTATGTGTGTGGGTTACCAGCAAGAATTAGAAGGCTAGAAGAAAGAGCTCGAACACGGGCAAAAGAAGGTCCTACAGTTCCGTTCAGTTGGATTTTCAATCGGGAGGTTAAGCTGTAGTACGTGCAAAGAAGCAAACGAATGATTAATCGCCACTATCCAAAGGATAAGAAAATAGCAATTCGTGTTCTAGTGAAAAAATATCGTGTGATGGAGTCAATTAGGGATCCGAAGTAGGAGAGTTTAAGCGTTCTCTACGTTTTTCTTCTTTTTGTGTAACTTGCGTCAGCTGTAATATCTGGTTCCGCAGAATAACAAAATAATCTAAACATTATACATTATAAATATGTTCTTATATACAAATCTGTGTCAGAAATTTAACGAAATTAGTGCCCAAATTCCTATTTGGGTTTTGAATTTATAATCAATCTTCTGTTTTAGTTGTATTCAAAATTGGGTGGCAGGCACCCGACCCATCATACAGTGCACGTTCGGCCACCGTCAAACACCCTCGGGGGGGGGTCACCCATGTGCGGCGCCACAATCCATTGCGGACACTGTTTCCTTCTACGTTCCAACCTTTCCCCATATCTAGAGATTGCACTTCAACTACTAATTATTAAACTAAAAAAAGAAAAAAAAAATCACTTAGTAAGAACTTTTTTTTTTTTTTGGTCGTATGAAGAAATAAGAGATTGGGAAGGAAGGCTGAAAGGATTTTAAAAAATCTGACATAAACTCAAAATACTGATCGAATCTGAAATTAGTAAAAGGGACTCGTAAGCTATGAATGAGGATCGAATTCGAAAAATCAATAACATCGCTAATCTTTTAGAGATCAGCACCTGGAATCTAAATGAGCTACCAAGAGTCCGACATTCGTTTGAACTTTCTGGATCTAATTTCATGTGGAAAATCCAGAAAATGAATAGAAGAAAAACAGCAGAAACGGAGATTTGGTCAGATAAAAGACTTGCGAATCAAAACATGAAAAATGATGAACAGAAGAAGAGAATGAGATCGAAATGAATGTAGCGACTTGTTCGAGGCCATAATCAAAACTAACGAAATTTGACAGGCACGGAGAACCGCTAACGTTTATATGGCCAACCTTCAATGCAATTCACGCCATTATAAATCGACGCCCTTGCTCTGCAAATTGGCTTCCCTCCATTGTTCTTCTTCCATTCAAGCAAATCGAAATTCAAAAACATTTTTCATTTTTTTCTTCAAAAGAGAGATAAGGAAAAACTAGAACAGACCATTTCGAAAGAAACAACCCTAAACGAAAACGCTGAGAATAACGAAGAAGAAACCCTAGAAATTGGAACTCGGAGAAGATCACCGCAAGAGGATTATGAACAACTTCGATGTTGAAGGCGGGTTTTATAGGCTCTGAAGAAGATTAGCGAAAAGAAAACCCTAACCGCATTAGATTGAATTACAAATAAGCCCTCACGAGTTCAATAAAACTCGGTCTCGGTTACTCACCAAGAAGATGGGCCTACTTTCTAAACGTTCTCTAGTTATTGGGCTTTTGAGCCCAATAACTTCAAAGCTCATATACAACAAATATCTCATCTCGGCCCAACTCGTATGAAGCCCATTTCCCAATCCAACCATATTCAAACAAATTTGTATGTACTCACCCAAAAAACAAAGGGGCAAATTTATAAGTATAACGCAACACAACTATTACAAAAAGAATAATTATAGAATAATTGATGTGAATATTTTGGACCTAACAAATGAATTTAGAAAACACTCAGTATGTAGTGTAATGGTTGTAGAGGAGAACCAATAACCTACATTCCCAGCAAGAGTGTGTAAACTTCTTCCTATATCATATGCATTTTAAAATCGAGAGTCTGACGGCGATACGTAACGGACCAAAACAAACAATATCTACTTACTATGAGTGAGTTTGGGTTGTTACAAAGGGTGGAATCCTCATCCCACATTGGTCGGAGAGGAAATCGAAGTCTTCCTTATAAAGGTGTTAGCAAACCTCTCCCTATTAGTTATGCTTTAAAATTTAAAGTAGACAAGTGAAAAAACTTTTGTTATGCTTCAAATTTAAAGGTGTTGATAGTGTTCGAATATGTTCGTTAAACGTAGTTCCATTTTTCAAAAGTTTCAATATTTTATCTCCTTTATATTCGAATATATTTCAATTTTGTATTTCACGGGTATAAATTTAGATAAGAAACTATTTAAAAAATATTATACCATGCAAGTTTACTATCAACATGCAATACTGCAAGACAATATTGATTTAGTATTTGCAAAGAACTCTACATCTTTTCTGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGAAAAGGCAATGAAATTAGCTCACAATTTTTCTTGACCAAGTGATCCAAGCAGTCATCTATTTGAACATGCTATACTGTTAGTGTACCTGGATCAACAATCAAGATCAGATCAGATCAGAAGGGGCTTTCATCAACAAGCATTAA

mRNA sequence

ATGCCGCAGCTTGCGAGTTTCAGATCTCCTAAGTTCGTTGTGGCCTCCACTCTCCGCTCCACTTCCAGGGAGGTCGAGGCCCTTAAGAAACCCTTTATACCTCCTAGGGAAGTGCATCCTCAAGTAACTCACTCAATGCCACCACAGAAGATAGAGATGTTTAAATGTCTGGAGGATTGGGCAGCAGAGAACATTTTAGTTTATTTGAAACCAGTGGAGAGGTGTTGGCAACCACTGGATTTGTTGCCAGATCCGGCATCTGAAGGATTTCAGGAGCAAGTGATGGAGCTTAGAGAGAGGGCAAAGGAAGTCCCTGATGATTACTTCATCGTTTTAGTTGGAGACATGATTACAGAAGATGCCCTTCCTACTTACCAGACAATGCTTAATACCATGGATGGAGTTAGGGATGAAACTGGAGCAAGCCTCAGTTCTTGGGCCATTTGGACGAGGTCTTGGACTGCTGAAGAGAACAGGCATGGTGACGTACTAAACAAATATCTTTACCTATCAGGGCGAGTTGATATGAGGCAGGTTGAGAAAACTATTCAGTATTTGATTCGATCAGGAATGGACCCAGGAACTGAAAACAACCCTTACCTAGGATTTATATACACCTCATTTCAAGAGAGAGCAACCTTCATCTCCCATGGGAACACTGCCAGATTAGCAAAGGAGCACGGAGACATAAAGTTGGCCCAATTATGTGGAACAATTGCTGCAGATGAGAAGCGACACGAAGCTGCATATACAAAAATAGTTGAAAAGCTGTTTGAGATAGACCCTGAAGGAACCATCCTGTGTTTTGAAGACATGATGAGGAAGAACATTACAATGCCAGCCCACATGATGTACGATGGCCGTGACAACAACCTCTTCAATCACTTCTCGGCACTTGCTCAGAGGCTGGGGGTTTATACAGCCAAGGACTACGCCGACATATTGGAGTATTTGGTTGGGAGATGGAAGGTGGAACAGCTCAAAGGACTGTCAGGTCACGGGCAGAAGGCTCAGGATTATGTGTGTGGGTTACCAGCAAGAATTAGAAGGCTAGAAGAAAGAGCTCGAACACGGGCAAAAGAAGGTCCTACAGTTCCGTTCAGTTGGATTTTCAATCGGGAGTGTACCTGGATCAACAATCAAGATCAGATCAGATCAGAAGGGGCTTTCATCAACAAGCATTAA

Coding sequence (CDS)

ATGCCGCAGCTTGCGAGTTTCAGATCTCCTAAGTTCGTTGTGGCCTCCACTCTCCGCTCCACTTCCAGGGAGGTCGAGGCCCTTAAGAAACCCTTTATACCTCCTAGGGAAGTGCATCCTCAAGTAACTCACTCAATGCCACCACAGAAGATAGAGATGTTTAAATGTCTGGAGGATTGGGCAGCAGAGAACATTTTAGTTTATTTGAAACCAGTGGAGAGGTGTTGGCAACCACTGGATTTGTTGCCAGATCCGGCATCTGAAGGATTTCAGGAGCAAGTGATGGAGCTTAGAGAGAGGGCAAAGGAAGTCCCTGATGATTACTTCATCGTTTTAGTTGGAGACATGATTACAGAAGATGCCCTTCCTACTTACCAGACAATGCTTAATACCATGGATGGAGTTAGGGATGAAACTGGAGCAAGCCTCAGTTCTTGGGCCATTTGGACGAGGTCTTGGACTGCTGAAGAGAACAGGCATGGTGACGTACTAAACAAATATCTTTACCTATCAGGGCGAGTTGATATGAGGCAGGTTGAGAAAACTATTCAGTATTTGATTCGATCAGGAATGGACCCAGGAACTGAAAACAACCCTTACCTAGGATTTATATACACCTCATTTCAAGAGAGAGCAACCTTCATCTCCCATGGGAACACTGCCAGATTAGCAAAGGAGCACGGAGACATAAAGTTGGCCCAATTATGTGGAACAATTGCTGCAGATGAGAAGCGACACGAAGCTGCATATACAAAAATAGTTGAAAAGCTGTTTGAGATAGACCCTGAAGGAACCATCCTGTGTTTTGAAGACATGATGAGGAAGAACATTACAATGCCAGCCCACATGATGTACGATGGCCGTGACAACAACCTCTTCAATCACTTCTCGGCACTTGCTCAGAGGCTGGGGGTTTATACAGCCAAGGACTACGCCGACATATTGGAGTATTTGGTTGGGAGATGGAAGGTGGAACAGCTCAAAGGACTGTCAGGTCACGGGCAGAAGGCTCAGGATTATGTGTGTGGGTTACCAGCAAGAATTAGAAGGCTAGAAGAAAGAGCTCGAACACGGGCAAAAGAAGGTCCTACAGTTCCGTTCAGTTGGATTTTCAATCGGGAGTGTACCTGGATCAACAATCAAGATCAGATCAGATCAGAAGGGGCTTTCATCAACAAGCATTAA

Protein sequence

MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDWAAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVEKTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIAADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALAQRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAKEGPTVPFSWIFNRECTWINNQDQIRSEGAFINKH
Homology
BLAST of CmoCh12G000650 vs. ExPASy Swiss-Prot
Match: P32061 (Acyl-[acyl-carrier-protein] desaturase, chloroplastic OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 1.9e-193
Identity = 320/374 (85.56%), Postives = 350/374 (93.58%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           MPQLAS RSPKFV+ASTLRSTSREVE LKKPF+PPREVH QVTHSMPPQK+E+FK LEDW
Sbjct: 20  MPQLASLRSPKFVMASTLRSTSREVETLKKPFMPPREVHLQVTHSMPPQKMEIFKSLEDW 79

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           A EN+LV+LKPVERCWQP D LPD A EGF EQV ELRERAKE+PD+YF+VLVGDMITE+
Sbjct: 80  AEENLLVHLKPVERCWQPQDFLPDSAFEGFHEQVRELRERAKELPDEYFVVLVGDMITEE 139

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNT+DGVRDETGAS + WAIWTR+WTAEENRHGD+LNKYLYLSGRVDMRQVE
Sbjct: 140 ALPTYQTMLNTLDGVRDETGASPTPWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQVE 199

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLI SGMDP TENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQ+CGTI 
Sbjct: 200 KTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQICGTIT 259

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           ADEKRHE AYTKIVEKLFEIDPEGT++ FE+MMRK ++MPAH+MYDGRD+NLF+HFSA+A
Sbjct: 260 ADEKRHETAYTKIVEKLFEIDPEGTVIAFEEMMRKKVSMPAHLMYDGRDDNLFHHFSAVA 319

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QRLGVYTAKDYADILE+LVGRWKVE L GLSG GQKAQDYVC LPARIR+LEERA+ RAK
Sbjct: 320 QRLGVYTAKDYADILEFLVGRWKVESLTGLSGEGQKAQDYVCALPARIRKLEERAQGRAK 379

Query: 361 EGPTVPFSWIFNRE 375
           EGPT+PFSWIF+R+
Sbjct: 380 EGPTIPFSWIFDRQ 393

BLAST of CmoCh12G000650 vs. ExPASy Swiss-Prot
Match: P22337 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 4.2e-188
Identity = 311/374 (83.16%), Postives = 347/374 (92.78%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           +P +AS RSPKF +ASTL+S S+EVE LKKPF+PPREVH QVTHSMPPQKIE+FK L++W
Sbjct: 20  LPPMASTRSPKFYMASTLKSGSKEVENLKKPFMPPREVHVQVTHSMPPQKIEIFKSLDNW 79

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           A ENILV+LKPVE+CWQP D LPDPAS+GF EQV ELRERAKE+PDDYF+VLVGDMITE+
Sbjct: 80  AEENILVHLKPVEKCWQPQDFLPDPASDGFDEQVRELRERAKEIPDDYFVVLVGDMITEE 139

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNT+DGVRDETGAS +SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMRQ+E
Sbjct: 140 ALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIE 199

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLI SGMDP TEN+PYLGFIYTSFQERATFISHGNTAR AKEHGDIKLAQ+CGTIA
Sbjct: 200 KTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICGTIA 259

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           ADEKRHE AYTKIVEKLFEIDP+GT+L F DMMRK I+MPAH+MYDGRD+NLF+HFSA+A
Sbjct: 260 ADEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMPAHLMYDGRDDNLFDHFSAVA 319

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QRLGVYTAKDYADILE+LVGRWKV++L GLS  GQKAQDYVC LP RIRRLEERA+ RAK
Sbjct: 320 QRLGVYTAKDYADILEFLVGRWKVDKLTGLSAEGQKAQDYVCRLPPRIRRLEERAQGRAK 379

Query: 361 EGPTVPFSWIFNRE 375
           E PT+PFSWIF+R+
Sbjct: 380 EAPTMPFSWIFDRQ 393

BLAST of CmoCh12G000650 vs. ExPASy Swiss-Prot
Match: Q42807 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Glycine max OX=3847 GN=ACPD PE=2 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.0e-185
Identity = 303/379 (79.95%), Postives = 347/379 (91.56%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           +PQ+AS RSP+F +ASTLRS S+EVE +KKPF PPREVH QVTHSMPPQKIE+F+ LEDW
Sbjct: 15  LPQMASLRSPRFRMASTLRSGSKEVENIKKPFTPPREVHVQVTHSMPPQKIEIFQSLEDW 74

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           A ENIL +LKPVE+CWQP D LPDP+S+GF+EQV ELRERAKE+PDDYF+VLVGDMITE+
Sbjct: 75  AEENILAHLKPVEKCWQPQDFLPDPSSDGFEEQVKELRERAKELPDDYFVVLVGDMITEE 134

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNT+DGVRDETGASL+SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDM+Q+E
Sbjct: 135 ALPTYQTMLNTLDGVRDETGASLTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMKQIE 194

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLI SGMDP TEN+PYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQ+CG IA
Sbjct: 195 KTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQICGMIA 254

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           +DEKRHE AYTKIVEKLFE DP+GT++ F DMMRK I MPAH+MYDGRD+NLF+++S++A
Sbjct: 255 SDEKRHETAYTKIVEKLFESDPDGTVMAFADMMRKKIAMPAHLMYDGRDDNLFDNYSSVA 314

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QR+GVYTAKDYADILE+LVGRWKVEQL GLSG G+KAQ+Y+CGLP RIRRLEERA+ R K
Sbjct: 315 QRIGVYTAKDYADILEFLVGRWKVEQLTGLSGEGRKAQEYICGLPPRIRRLEERAQARVK 374

Query: 361 EGPTVPFSWIFNRECTWIN 380
           E  T+ FSWI +RE T +N
Sbjct: 375 ESSTLKFSWIHDRESTTLN 393

BLAST of CmoCh12G000650 vs. ExPASy Swiss-Prot
Match: P22243 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Carthamus tinctorius OX=4222 PE=1 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 1.0e-181
Identity = 298/373 (79.89%), Postives = 338/373 (90.62%), Query Frame = 0

Query: 2   PQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDWA 61
           P+ A+ RSPKF +ASTL S++ +V+  KKPF PPREVH QVTHSMPPQKIE+FK +E WA
Sbjct: 21  PKKANLRSPKFAMASTLGSSTPKVDNAKKPFQPPREVHVQVTHSMPPQKIEIFKSIEGWA 80

Query: 62  AENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITEDA 121
            +NILV+LKPVE+CWQ  D LPDPASEGF EQV ELR RAKE+PDDYF+VLVGDMITE+A
Sbjct: 81  EQNILVHLKPVEKCWQAQDFLPDPASEGFDEQVKELRARAKEIPDDYFVVLVGDMITEEA 140

Query: 122 LPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVEK 181
           LPTYQTMLNT+DGVRDETGASL+ WA+WTR+WTAEENRHGD+L+ YLYLSGRVDMRQ++K
Sbjct: 141 LPTYQTMLNTLDGVRDETGASLTPWAVWTRAWTAEENRHGDLLHTYLYLSGRVDMRQIQK 200

Query: 182 TIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIAA 241
           TIQYLI SGMDP TEN+PYLGFIYTSFQERATF+SHGNTAR AK+HGD+KLAQ+CGTIA+
Sbjct: 201 TIQYLIGSGMDPRTENSPYLGFIYTSFQERATFVSHGNTARHAKDHGDVKLAQICGTIAS 260

Query: 242 DEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALAQ 301
           DEKRHE AYTKIVEKLFEIDP+GT+L F DMMRK I+MPAH+MYDGRD+NLF HFSA+AQ
Sbjct: 261 DEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMPAHLMYDGRDDNLFEHFSAVAQ 320

Query: 302 RLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAKE 361
           RLGVYTAKDYADILE+LVGRWKV  L GLSG G+KAQDYVCGLP RIRRLEERA+ RAKE
Sbjct: 321 RLGVYTAKDYADILEFLVGRWKVADLTGLSGEGRKAQDYVCGLPPRIRRLEERAQGRAKE 380

Query: 362 GPTVPFSWIFNRE 375
           GP VPFSWIF+R+
Sbjct: 381 GPVVPFSWIFDRQ 393

BLAST of CmoCh12G000650 vs. ExPASy Swiss-Prot
Match: O22832 (Stearoyl-[acyl-carrier-protein] 9-desaturase 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAB2 PE=1 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 2.6e-177
Identity = 292/377 (77.45%), Postives = 338/377 (89.66%), Query Frame = 0

Query: 2   PQLASFRSPKFVVAST----LRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCL 61
           P   SFRSPKF+  ++    L S  +EVE+LKKPF PPREVH QV HSMPPQKIE+FK +
Sbjct: 22  PPTPSFRSPKFLCLASSSPALSSGPKEVESLKKPFTPPREVHVQVLHSMPPQKIEIFKSM 81

Query: 62  EDWAAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMI 121
           E+WA EN+L++LK VE+ WQP D LPDPAS+GF++QV ELRERA+E+PDDYF+VLVGDMI
Sbjct: 82  ENWAEENLLIHLKDVEKSWQPQDFLPDPASDGFEDQVRELRERARELPDDYFVVLVGDMI 141

Query: 122 TEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMR 181
           TE+ALPTYQTMLNT+DGVRDETGAS +SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMR
Sbjct: 142 TEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMR 201

Query: 182 QVEKTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCG 241
           Q+EKTIQYLI SGMDP TENNPYLGFIYTSFQERATFISHGNTAR AKEHGDIKLAQ+CG
Sbjct: 202 QIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICG 261

Query: 242 TIAADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFS 301
           TIAADEKRHE AYTKIVEKLFEIDP+GT++ F DMMRK I+MPAH+MYDGR++NLF++FS
Sbjct: 262 TIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFS 321

Query: 302 ALAQRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERART 361
           ++AQRLGVYTAKDYADILE+LVGRWK++ L GLSG G KAQDY+CGL  RI+RL+ERA+ 
Sbjct: 322 SVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNKAQDYLCGLAPRIKRLDERAQA 381

Query: 362 RAKEGPTVPFSWIFNRE 375
           RAK+GP +PFSWI +RE
Sbjct: 382 RAKKGPKIPFSWIHDRE 398

BLAST of CmoCh12G000650 vs. ExPASy TrEMBL
Match: A0A6J1GHE1 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC111453985 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.1e-219
Identity = 374/374 (100.00%), Postives = 374/374 (100.00%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW
Sbjct: 20  MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 79

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED
Sbjct: 80  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 139

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE
Sbjct: 140 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 199

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA
Sbjct: 200 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 259

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA
Sbjct: 260 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 319

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK
Sbjct: 320 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 379

Query: 361 EGPTVPFSWIFNRE 375
           EGPTVPFSWIFNRE
Sbjct: 380 EGPTVPFSWIFNRE 393

BLAST of CmoCh12G000650 vs. ExPASy TrEMBL
Match: A0A6J1KQ22 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC111496572 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.2e-207
Identity = 350/374 (93.58%), Postives = 365/374 (97.59%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW
Sbjct: 20  MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 79

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           AAENILV+LKPVERCWQP D LPDPASEGFQEQVMELRERAKEVPDDYF+VLVGDMITED
Sbjct: 80  AAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERAKEVPDDYFVVLVGDMITED 139

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNTMDGVRDETGASL+SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMRQVE
Sbjct: 140 ALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQVE 199

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLI SGMDPGTENNPYLGFIYTSFQERATFISHGNTARLA E+GDIKLAQLCGTIA
Sbjct: 200 KTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAMENGDIKLAQLCGTIA 259

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           ADEKRHE AYTKIVEKLFEIDPEGT+LCFEDMMRKNITMPAHMMYDGRDNNLF+HFSA+A
Sbjct: 260 ADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPAHMMYDGRDNNLFDHFSAVA 319

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QRLGVYTAKDYA+I+E+LVGRWKVE+L GLSG GQKAQDYVCGLPARIRRLEERA+ RAK
Sbjct: 320 QRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYVCGLPARIRRLEERAQARAK 379

Query: 361 EGPTVPFSWIFNRE 375
           EG T+PFSWIFNRE
Sbjct: 380 EGRTIPFSWIFNRE 393

BLAST of CmoCh12G000650 vs. ExPASy TrEMBL
Match: A0A1S3BAE5 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103487766 PE=3 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.7e-195
Identity = 325/374 (86.90%), Postives = 356/374 (95.19%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           MPQLAS RSPKFV+ASTLRSTSREVE LKKPF+PPREVH QVTHSMPPQKIE+FK LEDW
Sbjct: 20  MPQLASLRSPKFVMASTLRSTSREVETLKKPFMPPREVHLQVTHSMPPQKIEIFKSLEDW 79

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           A EN+LV+LKPVERCWQP D LPDPA +GF EQVMELRERAKE+PDDYF+VLVGDMITE+
Sbjct: 80  AEENLLVHLKPVERCWQPQDFLPDPAFDGFHEQVMELRERAKEIPDDYFVVLVGDMITEE 139

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNT+DGVRDETGAS +SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMRQ+E
Sbjct: 140 ALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIE 199

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLI SGMDP TENNPYLGFIYTSFQERATF+SHGNTARLAKEHGDIKLAQ+CGTIA
Sbjct: 200 KTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFVSHGNTARLAKEHGDIKLAQICGTIA 259

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           ADEKRHE AYTKIVEKLFEIDPEGT++ FEDMMRK ++MPAH+MYDGRD+NLF+HFSA+A
Sbjct: 260 ADEKRHETAYTKIVEKLFEIDPEGTVIAFEDMMRKKVSMPAHLMYDGRDDNLFDHFSAVA 319

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QRLGVYTAKDYADILE+LVGRWKVE L GLSG GQKAQDYVCGLPARIR+LEERA++RAK
Sbjct: 320 QRLGVYTAKDYADILEFLVGRWKVESLTGLSGEGQKAQDYVCGLPARIRKLEERAQSRAK 379

Query: 361 EGPTVPFSWIFNRE 375
           EGPT+PFSWIF+R+
Sbjct: 380 EGPTIPFSWIFDRQ 393

BLAST of CmoCh12G000650 vs. ExPASy TrEMBL
Match: A0A6J1HDY5 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC111462685 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.4e-194
Identity = 326/374 (87.17%), Postives = 353/374 (94.39%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           MPQLAS RSPKFV+ASTL STSREVE LKKPF+PPREVH QVTHSMPPQKIE+FK LEDW
Sbjct: 20  MPQLASLRSPKFVMASTLHSTSREVETLKKPFMPPREVHLQVTHSMPPQKIEIFKSLEDW 79

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           A ENIL +LKPVERCWQP D LPDPASEGF EQVMELRERAKE+PDDYF+VLVGDMITE+
Sbjct: 80  AEENILCHLKPVERCWQPQDFLPDPASEGFHEQVMELRERAKELPDDYFVVLVGDMITEE 139

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNT+DGVRDETGAS +SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMRQ+E
Sbjct: 140 ALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIE 199

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLI SGMDP TENNPYLGFIYTSFQERATFISHGNTARLAK+HGDIKLAQ+CGTIA
Sbjct: 200 KTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTARLAKDHGDIKLAQICGTIA 259

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           ADEKRHE AYTKIVEKLFEIDPEGT++ FEDMMRK ++MPAH+MYDGRD+NLF+HFSA+A
Sbjct: 260 ADEKRHETAYTKIVEKLFEIDPEGTVIAFEDMMRKKVSMPAHLMYDGRDDNLFDHFSAVA 319

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QRLGVYTAKDYADILE+LV RWKVE+L GLSG GQKAQDYVCGLPARIRRLEERA+ RAK
Sbjct: 320 QRLGVYTAKDYADILEFLVARWKVEKLTGLSGEGQKAQDYVCGLPARIRRLEERAQARAK 379

Query: 361 EGPTVPFSWIFNRE 375
           EGP +PFSWIF+RE
Sbjct: 380 EGPAIPFSWIFDRE 393

BLAST of CmoCh12G000650 vs. ExPASy TrEMBL
Match: A0A6J1BQY3 (Acyl-[acyl-carrier-protein] desaturase OS=Momordica charantia OX=3673 GN=LOC111004796 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.3e-194
Identity = 324/374 (86.63%), Postives = 354/374 (94.65%), Query Frame = 0

Query: 1   MPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 60
           +PQLAS RSPKF++ASTLRSTSREVE LKKPF+PPREVH QVTHSMPPQKIEMFK +E+W
Sbjct: 20  LPQLASLRSPKFLMASTLRSTSREVETLKKPFMPPREVHLQVTHSMPPQKIEMFKSMENW 79

Query: 61  AAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITED 120
           A ENILV+LKPVERCWQP D LPDPAS+GF EQVMELRERAKE+PDDYF+VLVGDMITE+
Sbjct: 80  AEENILVHLKPVERCWQPQDFLPDPASDGFHEQVMELRERAKEIPDDYFVVLVGDMITEE 139

Query: 121 ALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVE 180
           ALPTYQTMLNT+DGVRDETGASL+SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMRQ+E
Sbjct: 140 ALPTYQTMLNTLDGVRDETGASLTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIE 199

Query: 181 KTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIA 240
           KTIQYLI SGMDP TENNPYLGFIYTSFQERATFISHGNTAR AK+HGDIKLAQ+CGTIA
Sbjct: 200 KTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTARQAKQHGDIKLAQICGTIA 259

Query: 241 ADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALA 300
           ADEKRHE AYTKIVEKLFEIDPEGT++ FEDMMRK ++MPAH+MYDGRD+NLF+HFSA+A
Sbjct: 260 ADEKRHETAYTKIVEKLFEIDPEGTVIAFEDMMRKKVSMPAHLMYDGRDDNLFDHFSAVA 319

Query: 301 QRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAK 360
           QRLGVYTAKDYADILE+LVGRWKVE+L GLS  GQKAQDYVCGLPARIRRLEERA+ RAK
Sbjct: 320 QRLGVYTAKDYADILEFLVGRWKVEKLTGLSAEGQKAQDYVCGLPARIRRLEERAQGRAK 379

Query: 361 EGPTVPFSWIFNRE 375
           E  T+PFSWIFNRE
Sbjct: 380 EAATIPFSWIFNRE 393

BLAST of CmoCh12G000650 vs. TAIR 10
Match: AT2G43710.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 622.9 bits (1605), Expect = 1.8e-178
Identity = 292/377 (77.45%), Postives = 338/377 (89.66%), Query Frame = 0

Query: 2   PQLASFRSPKFVVAST----LRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCL 61
           P   SFRSPKF+  ++    L S  +EVE+LKKPF PPREVH QV HSMPPQKIE+FK +
Sbjct: 22  PPTPSFRSPKFLCLASSSPALSSGPKEVESLKKPFTPPREVHVQVLHSMPPQKIEIFKSM 81

Query: 62  EDWAAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMI 121
           E+WA EN+L++LK VE+ WQP D LPDPAS+GF++QV ELRERA+E+PDDYF+VLVGDMI
Sbjct: 82  ENWAEENLLIHLKDVEKSWQPQDFLPDPASDGFEDQVRELRERARELPDDYFVVLVGDMI 141

Query: 122 TEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMR 181
           TE+ALPTYQTMLNT+DGVRDETGAS +SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMR
Sbjct: 142 TEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMR 201

Query: 182 QVEKTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCG 241
           Q+EKTIQYLI SGMDP TENNPYLGFIYTSFQERATFISHGNTAR AKEHGDIKLAQ+CG
Sbjct: 202 QIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICG 261

Query: 242 TIAADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFS 301
           TIAADEKRHE AYTKIVEKLFEIDP+GT++ F DMMRK I+MPAH+MYDGR++NLF++FS
Sbjct: 262 TIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFS 321

Query: 302 ALAQRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERART 361
           ++AQRLGVYTAKDYADILE+LVGRWK++ L GLSG G KAQDY+CGL  RI+RL+ERA+ 
Sbjct: 322 SVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNKAQDYLCGLAPRIKRLDERAQA 381

Query: 362 RAKEGPTVPFSWIFNRE 375
           RAK+GP +PFSWI +RE
Sbjct: 382 RAKKGPKIPFSWIHDRE 398

BLAST of CmoCh12G000650 vs. TAIR 10
Match: AT2G43710.2 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 620.9 bits (1600), Expect = 6.9e-178
Identity = 291/377 (77.19%), Postives = 337/377 (89.39%), Query Frame = 0

Query: 2   PQLASFRSPKFVVAST----LRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCL 61
           P   SFRSPKF+  ++    L S  + VE+LKKPF PPREVH QV HSMPPQKIE+FK +
Sbjct: 22  PPTPSFRSPKFLCLASSSPALSSGPKSVESLKKPFTPPREVHVQVLHSMPPQKIEIFKSM 81

Query: 62  EDWAAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMI 121
           E+WA EN+L++LK VE+ WQP D LPDPAS+GF++QV ELRERA+E+PDDYF+VLVGDMI
Sbjct: 82  ENWAEENLLIHLKDVEKSWQPQDFLPDPASDGFEDQVRELRERARELPDDYFVVLVGDMI 141

Query: 122 TEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMR 181
           TE+ALPTYQTMLNT+DGVRDETGAS +SWAIWTR+WTAEENRHGD+LNKYLYLSGRVDMR
Sbjct: 142 TEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMR 201

Query: 182 QVEKTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCG 241
           Q+EKTIQYLI SGMDP TENNPYLGFIYTSFQERATFISHGNTAR AKEHGDIKLAQ+CG
Sbjct: 202 QIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTARQAKEHGDIKLAQICG 261

Query: 242 TIAADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFS 301
           TIAADEKRHE AYTKIVEKLFEIDP+GT++ F DMMRK I+MPAH+MYDGR++NLF++FS
Sbjct: 262 TIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFS 321

Query: 302 ALAQRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERART 361
           ++AQRLGVYTAKDYADILE+LVGRWK++ L GLSG G KAQDY+CGL  RI+RL+ERA+ 
Sbjct: 322 SVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNKAQDYLCGLAPRIKRLDERAQA 381

Query: 362 RAKEGPTVPFSWIFNRE 375
           RAK+GP +PFSWI +RE
Sbjct: 382 RAKKGPKIPFSWIHDRE 398

BLAST of CmoCh12G000650 vs. TAIR 10
Match: AT3G02630.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 588.6 bits (1516), Expect = 3.8e-168
Identity = 281/372 (75.54%), Postives = 319/372 (85.75%), Query Frame = 0

Query: 3   QLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDWAA 62
           Q    RS +  +AST+RS + EV   +K +IPPREVH QV HSMPPQK+E+FK LE WA 
Sbjct: 21  QARGSRSSRVSMASTIRSATTEVTNGRKLYIPPREVHVQVKHSMPPQKLEIFKSLEGWAD 80

Query: 63  ENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITEDAL 122
           E +L YLKPVE+ WQP D LP+P SEGF +QV ELRER KE+PDDYF+VLVGDMITE+AL
Sbjct: 81  ETLLTYLKPVEKSWQPTDFLPEPESEGFYDQVKELRERCKELPDDYFVVLVGDMITEEAL 140

Query: 123 PTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVEKT 182
           PTYQTMLNT+DGVRDETGAS + WAIWTR+WTAEENRHGD+LNKYLYLSGRVDMRQ+EKT
Sbjct: 141 PTYQTMLNTLDGVRDETGASPTPWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKT 200

Query: 183 IQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIAAD 242
           IQYLI SGMDP TENNPYLGFIYTSFQERATFISHGNTARLAK+ GD+KLAQ+CGTIAAD
Sbjct: 201 IQYLIGSGMDPKTENNPYLGFIYTSFQERATFISHGNTARLAKDRGDLKLAQICGTIAAD 260

Query: 243 EKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALAQR 302
           E+RHE AYTKIVEKLFEIDP+GTIL   DMM+K I+MPAH+MYDG+D+NLF HFS +AQR
Sbjct: 261 ERRHETAYTKIVEKLFEIDPDGTILGLADMMKKKISMPAHLMYDGQDDNLFEHFSTVAQR 320

Query: 303 LGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAKE- 362
           LGVYTAKDYADILE+LV RW VE L  LS  G +AQD+VCGLPARIR++EERA+ RAKE 
Sbjct: 321 LGVYTAKDYADILEFLVERWNVETLTDLSSEGHRAQDFVCGLPARIRKIEERAQGRAKEA 380

Query: 363 GPTVPFSWIFNR 374
              +PFSWIF R
Sbjct: 381 AKNIPFSWIFGR 392

BLAST of CmoCh12G000650 vs. TAIR 10
Match: AT5G16240.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 564.7 bits (1454), Expect = 5.9e-161
Identity = 269/359 (74.93%), Postives = 311/359 (86.63%), Query Frame = 0

Query: 17  TLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDWAAENILVYLKPVERCW 76
           T+RS S  V   +K +IPPREVH QV +SMPPQK+E+FK LE WA +N+L YLKPVE+ W
Sbjct: 35  TIRSDSTAVG--RKLYIPPREVHLQVKYSMPPQKLEIFKSLEGWANDNLLAYLKPVEKSW 94

Query: 77  QPLDLLPDPASEGFQEQVMELRERAKEVPDDYFIVLVGDMITEDALPTYQTMLNTMDGVR 136
           QP D LP+P SEGF +QV ELRER KE+ DDY IVLVGDMITE+ALPTYQTM+NT+DGVR
Sbjct: 95  QPTDFLPEPESEGFYDQVKELRERCKELSDDYLIVLVGDMITEEALPTYQTMINTLDGVR 154

Query: 137 DETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQVEKTIQYLIRSGMDPGTE 196
           DETGAS + WA+WTR+WTAEENRHGD+LNKYLYLSGRVDMRQ+EKTIQYLI SGMDP TE
Sbjct: 155 DETGASPTPWAVWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPKTE 214

Query: 197 NNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTIAADEKRHEAAYTKIVEK 256
           NNPYLGFIYTSFQERATFISHGNTARLAK+ GD+ L ++CGTIAADE+RHE AYTKIVEK
Sbjct: 215 NNPYLGFIYTSFQERATFISHGNTARLAKDLGDLTLGKICGTIAADERRHEHAYTKIVEK 274

Query: 257 LFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSALAQRLGVYTAKDYADILE 316
           LFEIDP+ T++ F DMMRK I+MPAH+MYDGRD+NLF+HFS++AQRLGVYTAKDYADIL+
Sbjct: 275 LFEIDPDTTVVGFADMMRKKISMPAHLMYDGRDDNLFDHFSSVAQRLGVYTAKDYADILQ 334

Query: 317 YLVGRWKVEQLKGLSGHGQKAQDYVCGLPARIRRLEERARTRAKE-GPTVPFSWIFNRE 375
           +LV RW VE+L  LS  G +AQDY+CGLPARIR+LEERA+ R KE    +PFSWIF RE
Sbjct: 335 HLVERWNVEKLSDLSSEGNRAQDYLCGLPARIRKLEERAQGRTKEAAKNIPFSWIFGRE 391

BLAST of CmoCh12G000650 vs. TAIR 10
Match: AT3G02610.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 513.5 bits (1321), Expect = 1.6e-145
Identity = 253/380 (66.58%), Postives = 304/380 (80.00%), Query Frame = 0

Query: 5   ASFRSPKFV----VASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 64
           +SF  P+ +    VA+    TS E +  KK F P +EV  QVTH++  +K+E+FK +E+W
Sbjct: 33  SSFSPPRLLRVSCVATNPSKTSEETD--KKKFRPIKEVPNQVTHTITQEKLEIFKSMENW 92

Query: 65  AAENILVYLKPVERCWQPLDLLPDPASEG-FQEQVMELRERAKEVPDDYFIVLVGDMITE 124
           A EN+L YLKPVE  WQP D LP+   E  F EQV ELR+R KE+PDDYF+VLVGDMITE
Sbjct: 93  AQENLLSYLKPVEASWQPQDFLPETNDEDRFYEQVKELRDRTKEIPDDYFVVLVGDMITE 152

Query: 125 DALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHGDVLNKYLYLSGRVDMRQV 184
           +ALPTYQT LNT+DGV+DETG SL+ WA+W R+WTAEENRHGD+LNKYLYLSGRVDMR V
Sbjct: 153 EALPTYQTTLNTLDGVKDETGGSLTPWAVWVRAWTAEENRHGDLLNKYLYLSGRVDMRHV 212

Query: 185 EKTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAKEHGDIKLAQLCGTI 244
           EKTIQYLI SGMD   ENNPY GFIYTSFQERATFISHGNTA+LA  +GD  LA++CGTI
Sbjct: 213 EKTIQYLIGSGMDSKFENNPYNGFIYTSFQERATFISHGNTAKLATTYGDTTLAKICGTI 272

Query: 245 AADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPAHMMYDGRDNNLFNHFSAL 304
           AADEKRHE AYT+IVEKLFEIDP+GT+     MMRK ITMPAH+M+DGRD++LF+H++A+
Sbjct: 273 AADEKRHETAYTRIVEKLFEIDPDGTVQALASMMRKRITMPAHLMHDGRDDDLFDHYAAV 332

Query: 305 AQRLGVYTAKDYADILEYLVGRWKVEQL-KGLSGHGQKAQDYVCGLPARIRRLEERARTR 364
           AQR+GVYTA DYA ILE+L+ RW+VE+L  GLSG G++AQDY+C LP RIRRLEERA  R
Sbjct: 333 AQRIGVYTATDYAGILEFLLRRWEVEKLGMGLSGEGRRAQDYLCTLPQRIRRLEERANDR 392

Query: 365 ----AKEGPTVPFSWIFNRE 375
               +K  P+V FSWI+ RE
Sbjct: 393 VKLASKSKPSVSFSWIYGRE 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P320611.9e-19385.56Acyl-[acyl-carrier-protein] desaturase, chloroplastic OS=Cucumis sativus OX=3659... [more]
P223374.2e-18883.16Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Ricinus communis ... [more]
Q428072.0e-18579.95Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Glycine max OX=38... [more]
P222431.0e-18179.89Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Carthamus tinctor... [more]
O228322.6e-17777.45Stearoyl-[acyl-carrier-protein] 9-desaturase 7, chloroplastic OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A6J1GHE12.1e-219100.00Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1KQ224.2e-20793.58Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC1114965... [more]
A0A1S3BAE51.7e-19586.90Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103487766 P... [more]
A0A6J1HDY51.4e-19487.17Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1BQY35.3e-19486.63Acyl-[acyl-carrier-protein] desaturase OS=Momordica charantia OX=3673 GN=LOC1110... [more]
Match NameE-valueIdentityDescription
AT2G43710.11.8e-17877.45Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT2G43710.26.9e-17877.19Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT3G02630.13.8e-16875.54Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT5G16240.15.9e-16174.93Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT3G02610.11.6e-14566.58Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005067Fatty acid desaturase, type 2PFAMPF03405FA_desaturase_2coord: 50..371
e-value: 2.9E-154
score: 512.8
IPR005067Fatty acid desaturase, type 2PIRSFPIRSF000346Dlt9_acylACP_descoord: 3..377
e-value: 9.7E-178
score: 588.5
IPR005067Fatty acid desaturase, type 2PANTHERPTHR31155ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATEDcoord: 6..375
IPR005067Fatty acid desaturase, type 2CDDcd01050Acyl_ACP_Desatcoord: 49..355
e-value: 9.82119E-179
score: 497.175
IPR012348Ribonucleotide reductase-likeGENE3D1.10.620.20Ribonucleotide Reductase, subunit Acoord: 32..376
e-value: 1.4E-172
score: 575.4
NoneNo IPR availablePANTHERPTHR31155:SF33ACYL-[ACYL-CARRIER-PROTEIN] DESATURASEcoord: 6..375
IPR005803Fatty acid desaturase type 2, conserved sitePROSITEPS00574FATTY_ACID_DESATUR_2coord: 297..316
IPR009078Ferritin-like superfamilySUPERFAMILY47240Ferritin-likecoord: 35..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh12G000650.1CmoCh12G000650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006631 fatty acid metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0045300 acyl-[acyl-carrier-protein] desaturase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0102786 stearoyl-[acp] desaturase activity
molecular_function GO:0004768 stearoyl-CoA 9-desaturase activity
molecular_function GO:0016491 oxidoreductase activity