CmoCh12G000530 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh12G000530
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Protein kish
Location	Cmo_Chr12: 277213 .. 278511 (+)
RNA-Seq Expression	CmoCh12G000530
Synteny	CmoCh12G000530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAAAGGACCCTGACCTTTGGTGTATGGACTATGAAAATAGTCATAGCTTGGATAAATAGGAGCTATTAATCGCTGACATGGTCGATGTTTGTTTTTCAGTCGGCTTTGTTCAATTTTCATTCGTTTCTGACAGTAATCCTACTGGGAATTTGTACCTGTACCTATATGAAGATGCAGTTTCCTGCTATTCTTGAACAGAGAAATGGGTAATTTTTTCATTTCTGTTATGCTGTATAATACTGCATTGAGATAAATATAGATAATCAGGTTTCGAGCATGTTGCCTAATTGTTTTGAGTTTTCATTTCATGTTGCTACTTTCTTTTCCTTCTCTCAAACATAATTGGAATTAATTTGATGCTTGGAGAGACCCCCATTCTTTCCTTACCTCGTGAAATTGAATTTCCTATACATATGTTAGGTGTTGATTGATTTATTATCATGGTGGTGCTATTGATCACCGATATTGATGTTCTTGGTGTGCGAAACCACTACCTCTTATGTTCGAAATACGATTACATTATAAATTCCATATCGTTTTTAATCGAGTTGCTCATCTGACAGGTTCCGTGGCTTCTTTTGGAAAGCAGCTAGAATAGGTAGGATTCTTGGTTAAATTTGCACAATTGCTCTATTGCATGAAAATATTTTTCTGGAACAATTTATACATAAACTGTAGAACATCTGAACTCTTTCTTTTTTCTTCTCTCTTTGGTTCTTCCAGCATTCGCCTCTCTTAATGAGACTGAATTTCTGATGTATTGCACCCAAAAAAAAGTATTTAGCTTCTTTATTATTAAAAAAAACTCAGTAGCTTTTCGGCCTTTTAGATTTCTCTTGTTCATATACGAATTTATATACACAATCATGCAACGAGGGACATGGGAGAAAGAAACAAAGTCTAAGTTACACCTATTTCAATCAGTAGGGCATAGATTTTGTTGTCATTTGGCAAGTATAACAGTCATTATTATCTGACGGGTTGATGTTCTGTTTGTGAATCAGGTGAACGTTTGAGTCCTTGGGTAGCTGTGGGGTGCTTCTCAATGGGAGTCTCAATAATATTCTTCTGAGCTATGCCTATATTCCCTGTTTTGTGGTTTATTTAGCATGTGTGTGCCTTACAGTCTTATTAGTAAGAGGGTCTTTGAAGTTAGATTTCTGTAGTGACATGGTTGAAAGCTATTTAAATCCATTTATAAATACACGAGCATTTCATTACAAGTTGTTTTGCTTGCTATGCATCCTCAAAAATGCCAAAGAGGAAGATCATTGTTGTGTTACATTTCTCATCAG

mRNA sequence

ATGTCGGCTTTGTTCAATTTTCATTCGTTTCTGACAGTAATCCTACTGGGAATTTGTACCTGTACCTATATGAAGATGCAGTTTCCTGCTATTCTTGAACAGAGAAATGGGTTCCGTGGCTTCTTTTGGAAAGCAGCTAGAATAGGTGAACGTTTGAGTCCTTGGGTAGCTGTGGGGTGCTTCTCAATGGGAGTCTCAATAATATTCTTCTGAGCTATGCCTATATTCCCTGTTTTGTGGTTTATTTAGCATGTGTGTGCCTTACAGTCTTATTAGTAAGAGGGTCTTTGAAGTTAGATTTCTGTAGTGACATGGTTGAAAGCTATTTAAATCCATTTATAAATACACGAGCATTTCATTACAAGTTGTTTTGCTTGCTATGCATCCTCAAAAATGCCAAAGAGGAAGATCATTGTTGTGTTACATTTCTCATCAG

Coding sequence (CDS)

ATGTCGGCTTTGTTCAATTTTCATTCGTTTCTGACAGTAATCCTACTGGGAATTTGTACCTGTACCTATATGAAGATGCAGTTTCCTGCTATTCTTGAACAGAGAAATGGGTTCCGTGGCTTCTTTTGGAAAGCAGCTAGAATAGGTGAACGTTTGAGTCCTTGGGTAGCTGTGGGGTGCTTCTCAATGGGAGTCTCAATAATATTCTTCTGA

Protein sequence

MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGCFSMGVSIIFF

Homology

[Close]

BLAST of CmoCh12G000530 vs. ExPASy Swiss-Prot
Match: Q9VWH8 (Protein kish OS=Drosophila melanogaster OX=7227 GN=ksh PE=3 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 3.1e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQ-RNGFRGFFWKAARIGERLSPWVAVG 60
          MSALFNFHS L+VILL ICTC Y++  FP+++++ + GF G FWK ARIGER SPWV   
Sbjct: 1  MSALFNFHSLLSVILLLICTCAYLRSLFPSLIDRNKTGFMGTFWKLARIGERKSPWVGAA 60

Query: 61 CFSMGVSIIFF 71
          C  M  +++F+
Sbjct: 61 CLIMAFTVLFW 71

[Close]

BLAST of CmoCh12G000530 vs. ExPASy Swiss-Prot
Match: Q9CR64 (Protein kish-A OS=Mus musculus OX=10090 GN=Tmem167a PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.2e-15
Identity = 41/70 (58.57%), Postives = 51/70 (72.86%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQ-RNGFRGFFWKAARIGERLSPWVAVG 60
          MSA+FNF S LTVILL ICTC Y++   P+IL++ + G  G FWK ARIGER SP+VA+ 
Sbjct: 1  MSAIFNFQSLLTVILLLICTCAYIRSLAPSILDRNKTGLLGIFWKCARIGERKSPYVAIC 60

Query: 61 CFSMGVSIIF 70
          C  M  SI+F
Sbjct: 61 CIVMAFSILF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy Swiss-Prot
Match: Q148I3 (Protein kish-A OS=Bos taurus OX=9913 GN=TMEM167A PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.9e-15
Identity = 41/70 (58.57%), Postives = 51/70 (72.86%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQ-RNGFRGFFWKAARIGERLSPWVAVG 60
          MSA+FNF S LTVILL ICTC Y++   P++L++ + G  G FWK ARIGER SP+VAV 
Sbjct: 1  MSAIFNFQSLLTVILLLICTCAYIRSLAPSLLDRNKTGLLGIFWKCARIGERKSPYVAVC 60

Query: 61 CFSMGVSIIF 70
          C  M  SI+F
Sbjct: 61 CIVMAFSILF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy Swiss-Prot
Match: Q5BJC2 (Protein kish-A OS=Danio rerio OX=7955 GN=tmem167a PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.9e-15
Identity = 40/70 (57.14%), Postives = 52/70 (74.29%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQ-RNGFRGFFWKAARIGERLSPWVAVG 60
          MSA+FNF S LTVILL ICTC Y++   P++L++ + GF G FWK ARIGER SP+VA  
Sbjct: 1  MSAIFNFQSLLTVILLLICTCAYIRSLTPSLLDKNKTGFLGIFWKCARIGERKSPYVAFC 60

Query: 61 CFSMGVSIIF 70
          C  M ++I+F
Sbjct: 61 CIVMALTILF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy Swiss-Prot
Match: Q8TBQ9 (Protein kish-A OS=Homo sapiens OX=9606 GN=TMEM167A PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.9e-15
Identity = 41/70 (58.57%), Postives = 51/70 (72.86%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQ-RNGFRGFFWKAARIGERLSPWVAVG 60
          MSA+FNF S LTVILL ICTC Y++   P++L++ + G  G FWK ARIGER SP+VAV 
Sbjct: 1  MSAIFNFQSLLTVILLLICTCAYIRSLAPSLLDRNKTGLLGIFWKCARIGERKSPYVAVC 60

Query: 61 CFSMGVSIIF 70
          C  M  SI+F
Sbjct: 61 CIVMAFSILF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy TrEMBL
Match: A0A6J1GJV2 (Protein kish OS=Cucurbita moschata OX=3662 GN=LOC111454546 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.7e-32
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60
          MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC
Sbjct: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60

Query: 61 FSMGVSIIFF 71
          FSMGVSIIFF
Sbjct: 61 FSMGVSIIFF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy TrEMBL
Match: A0A6J1KKJ8 (Protein kish OS=Cucurbita maxima OX=3661 GN=LOC111496111 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 7.8e-32
Identity = 69/70 (98.57%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60
          MSALFNFHSFLTVILLGICTCTY+KMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC
Sbjct: 1  MSALFNFHSFLTVILLGICTCTYVKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60

Query: 61 FSMGVSIIFF 71
          FSMGVSIIFF
Sbjct: 61 FSMGVSIIFF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy TrEMBL
Match: A0A6J1HB53 (Protein kish OS=Cucurbita moschata OX=3662 GN=LOC111462406 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.0e-31
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60
          MSALFNFHSFLTVILLGICTCTY+KM FPAILEQRNGFRGFFWKAARIGERLSPWVAVGC
Sbjct: 1  MSALFNFHSFLTVILLGICTCTYVKMHFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60

Query: 61 FSMGVSIIFF 71
          FSMGVSIIFF
Sbjct: 61 FSMGVSIIFF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy TrEMBL
Match: A0A0A0KGV9 (Protein kish OS=Cucumis sativus OX=3659 GN=Csa_6G365220 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.0e-31
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60
          MSALFNFHSFLTVILLGICTCTY+KM FPAILEQRNGFRGFFWKAARIGERLSPWVAVGC
Sbjct: 1  MSALFNFHSFLTVILLGICTCTYVKMHFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60

Query: 61 FSMGVSIIFF 71
          FSMGVSIIFF
Sbjct: 61 FSMGVSIIFF 70

[Close]

BLAST of CmoCh12G000530 vs. ExPASy TrEMBL
Match: A0A6J1BQQ0 (Protein kish OS=Momordica charantia OX=3673 GN=LOC111004584 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.0e-31
Identity = 68/70 (97.14%), Postives = 69/70 (98.57%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60
          MSALFNFHSFLTVILLGICTCTY+KM FPAILEQRNGFRGFFWKAARIGERLSPWVAVGC
Sbjct: 1  MSALFNFHSFLTVILLGICTCTYVKMHFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60

Query: 61 FSMGVSIIFF 71
          FSMGVSIIFF
Sbjct: 61 FSMGVSIIFF 70

[Close]

BLAST of CmoCh12G000530 vs. TAIR 10
Match: AT5G20165.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1242 (InterPro:IPR009653); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 134.0 bits (336), Expect = 4.5e-32
Identity = 62/70 (88.57%), Postives = 66/70 (94.29%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARIGERLSPWVAVGC 60
          MSALFNFHSFLTV+LL ICTCTY+KMQFPAILEQ+ GFRGFFWKAARIGERLSPWV+ GC
Sbjct: 1  MSALFNFHSFLTVVLLVICTCTYLKMQFPAILEQKTGFRGFFWKAARIGERLSPWVSAGC 60

Query: 61 FSMGVSIIFF 71
          F MGVSIIFF
Sbjct: 61 FMMGVSIIFF 70

[Close]

BLAST of CmoCh12G000530 vs. TAIR 10
Match: AT5G20165.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1242 (InterPro:IPR009653); Has 285 Blast hits to 285 proteins in 131 species: Archae - 0; Bacteria - 0; Metazoa - 142; Fungi - 72; Plants - 44; Viruses - 0; Other Eukaryotes - 27 (source: NCBI BLink). )

HSP 1 Score: 94.7 bits (234), Expect = 3.1e-20
Identity = 46/57 (80.70%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNGFRGFFWKAARI-GERLSPWV 57
          MSALFNFHSFLTV+LL ICTCTY+KMQFPAILEQ+ GFRGFFWKAARI    L P V
Sbjct: 1  MSALFNFHSFLTVVLLVICTCTYLKMQFPAILEQKTGFRGFFWKAARIDSNALKPMV 57

[Close]

BLAST of CmoCh12G000530 vs. TAIR 10
Match: AT5G20165.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1242 (InterPro:IPR009653). )

HSP 1 Score: 81.6 bits (200), Expect = 2.7e-16
Identity = 46/80 (57.50%), Postives = 49/80 (61.25%), Query Frame = 0


Query: 1  MSALFNFHSFLTVILLGICTCTYMKMQFPAILEQRNG----------------------- 57
          MSALFNFHSFLTV+LL ICTCTY+KMQFPAILEQ+ G                       
Sbjct: 1  MSALFNFHSFLTVVLLVICTCTYLKMQFPAILEQKTGIVFCQIKDALSDFSAYCYFRLQM 60

The following BLAST results are available for this feature:

BLAST of CmoCh12G000530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9VWH8	3.1e-17	56.34	Protein kish OS=Drosophila melanogaster OX=7227 GN=ksh PE=3 SV=2	[more]
Q9CR64	2.2e-15	58.57	Protein kish-A OS=Mus musculus OX=10090 GN=Tmem167a PE=1 SV=1	[more]
Q148I3	2.9e-15	58.57	Protein kish-A OS=Bos taurus OX=9913 GN=TMEM167A PE=3 SV=1	[more]
Q5BJC2	2.9e-15	57.14	Protein kish-A OS=Danio rerio OX=7955 GN=tmem167a PE=3 SV=1	[more]
Q8TBQ9	2.9e-15	58.57	Protein kish-A OS=Homo sapiens OX=9606 GN=TMEM167A PE=1 SV=1	[more]

BLAST of CmoCh12G000530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJV2	2.7e-32	100.00	Protein kish OS=Cucurbita moschata OX=3662 GN=LOC111454546 PE=3 SV=1	[more]
A0A6J1KKJ8	7.8e-32	98.57	Protein kish OS=Cucurbita maxima OX=3661 GN=LOC111496111 PE=3 SV=1	[more]
A0A6J1HB53	3.0e-31	97.14	Protein kish OS=Cucurbita moschata OX=3662 GN=LOC111462406 PE=3 SV=1	[more]
A0A0A0KGV9	3.0e-31	97.14	Protein kish OS=Cucumis sativus OX=3659 GN=Csa_6G365220 PE=3 SV=1	[more]
A0A6J1BQQ0	3.0e-31	97.14	Protein kish OS=Momordica charantia OX=3673 GN=LOC111004584 PE=3 SV=1	[more]

BLAST of CmoCh12G000530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20165.1	4.5e-32	88.57	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT5G20165.2	3.1e-20	80.70	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT5G20165.3	2.7e-16	57.50	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009653	Protein kish	PFAM	PF06842	DUF1242	coord: 10..43 e-value: 2.1E-17 score: 62.8
None	No IPR available	PANTHER	PTHR13229	PROTEIN KISH-A	coord: 1..70
None	No IPR available	PANTHER	PTHR13229:SF15	PROTEIN KISH	coord: 1..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh12G000530.1	CmoCh12G000530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046907	intracellular transport
biological_process	GO:0009306	protein secretion
cellular_component	GO:0000139	Golgi membrane
cellular_component	GO:0016021	integral component of membrane