CmoCh11G013280 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh11G013280
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionallene oxide synthase 3-like
LocationCmo_Chr11: 9170165 .. 9171607 (-)
RNA-Seq ExpressionCmoCh11G013280
SyntenyCmoCh11G013280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCCTTCTTCTTCCTCTGAACTCCCTCTGAAACCCATTCCCGGCGGCTATGGCTTCCCCTTCCTTGGAGCAATCAAAGACCGATACGATTACTTCTACTTCCAAGGGAAAGACGAATTCTTCCGCTCCCGAGTCGCCAAATACCGCTCCACCGTCTTCCGCGCCAACATGCCGCCGGGCCCCTTCATCTCCTCCGACTCCAGAGTCGTCGTCCTTCTCGACGCTCTCACCTTTCAGATCCTTTTTGATACTTCTAAGGTCGAGAAACGCAATCTTCTCGATGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGATATTCGTACCTGCGCCTATTTGGACCCGTCGGAAACAGAGCACTCTGTTTTGAAAAAGCTCTTCCTCTGTTTTCTTGCATCTCGTCACGACAGGTTTATCCCTCTGTTCCGAAGCTCCTTGTCGGAGATGTTTATTAAACTTGAAGATAAAATCGCTGAGAAGAATAAGGTCGTTGATTTCAACCCGATTAGTGATTCCATGTCGTTTGACTTCATATTCCGGCTGCTCTCCGACGGAACCCCTGACTCTAAACTGGCAGCCGATGGACCTGGAATGTTTGATAAATGGCTCGCTTTCCAGCTCGCCCCACTGGCTTCCATTGGTTTGCCGAAGATATTCTCAATTTTAGAAGATCTCGTCATTCACACCATGCCACTGCCTTTCTTTCTTGTCAAAAGTGGTTACATGAAGCTTTACAATGCGTTTTACTCCTGCTCTGATTCGTTTCTAGCCGAAGCGGAGAAACAGGGGATAAACAGAGAGAAAGCGTGTCACAATCTAGTGTTTCTGGCCGGATTCAATGCGTACGGCGGAATGAAAGTACTTTTTCCGATTTTGCTGAAGTGGATCGGCACCGCTGGGGAGGAGCTTCACCGGAATCTCGCCGACGAAATCAGAGCCGCCGTGAAGGAAGAAGGGGGATTGACATTTAGTGCAATAGAGAAGATGAGTCTATTGAAGTCCGTCGTGTACGAAGCGCTGAGGATCGAGCCGCCGGTGCCGTTCCAGTACGCGAAAGCGAAGGAGGACATCGTGGTACAGAGCCACGATTCCGCTTTCAAGATAAAGAAAGGGGAGATCATTTTTGGGTATCAGCCGTTTGCAACTAAAGATGGAAAGGTTTTTGAGAATCCGGAGAAGTTCATCGGCGACCGGTTCGTCGGAGAAGAAGGGGAGAAGCTTCTGAAGTATGTGTACTGGTCGAACGGTAGGGAGACAGAGGAGCCGACGCCGGAGGACAAGATGTGTCCGGGGAAAAATCTGGTGGTGCTGATGGCCAGAGTTATTGTAGTGGAATTCTTCCTCCGTTACGACACGTTCTCCGTCGAGGTCAACGATTTGCCGTTGGGTCCGTCGGTGAAGATCACGTCCGTCACGAAAGCCGCCGTGAAGGTCTGA

mRNA sequence

ATGGCTTCCCCTTCTTCTTCCTCTGAACTCCCTCTGAAACCCATTCCCGGCGGCTATGGCTTCCCCTTCCTTGGAGCAATCAAAGACCGATACGATTACTTCTACTTCCAAGGGAAAGACGAATTCTTCCGCTCCCGAGTCGCCAAATACCGCTCCACCGTCTTCCGCGCCAACATGCCGCCGGGCCCCTTCATCTCCTCCGACTCCAGAGTCGTCGTCCTTCTCGACGCTCTCACCTTTCAGATCCTTTTTGATACTTCTAAGGTCGAGAAACGCAATCTTCTCGATGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGATATTCGTACCTGCGCCTATTTGGACCCGTCGGAAACAGAGCACTCTGTTTTGAAAAAGCTCTTCCTCTGTTTTCTTGCATCTCGTCACGACAGGTTTATCCCTCTGTTCCGAAGCTCCTTGTCGGAGATGTTTATTAAACTTGAAGATAAAATCGCTGAGAAGAATAAGGTCGTTGATTTCAACCCGATTAGTGATTCCATGTCGTTTGACTTCATATTCCGGCTGCTCTCCGACGGAACCCCTGACTCTAAACTGGCAGCCGATGGACCTGGAATGTTTGATAAATGGCTCGCTTTCCAGCTCGCCCCACTGGCTTCCATTGGTTTGCCGAAGATATTCTCAATTTTAGAAGATCTCGTCATTCACACCATGCCACTGCCTTTCTTTCTTGTCAAAAGTGGTTACATGAAGCTTTACAATGCGTTTTACTCCTGCTCTGATTCGTTTCTAGCCGAAGCGGAGAAACAGGGGATAAACAGAGAGAAAGCGTGTCACAATCTAGTGTTTCTGGCCGGATTCAATGCGTACGGCGGAATGAAAGTACTTTTTCCGATTTTGCTGAAGTGGATCGGCACCGCTGGGGAGGAGCTTCACCGGAATCTCGCCGACGAAATCAGAGCCGCCGTGAAGGAAGAAGGGGGATTGACATTTAGTGCAATAGAGAAGATGAGTCTATTGAAGTCCGTCGTGTACGAAGCGCTGAGGATCGAGCCGCCGGTGCCGTTCCAGTACGCGAAAGCGAAGGAGGACATCGTGGTACAGAGCCACGATTCCGCTTTCAAGATAAAGAAAGGGGAGATCATTTTTGGGTATCAGCCGTTTGCAACTAAAGATGGAAAGGTTTTTGAGAATCCGGAGAAGTTCATCGGCGACCGGTTCGTCGGAGAAGAAGGGGAGAAGCTTCTGAAGTATGTGTACTGGTCGAACGGTAGGGAGACAGAGGAGCCGACGCCGGAGGACAAGATGTGTCCGGGGAAAAATCTGGTGGTGCTGATGGCCAGAGTTATTGTAGTGGAATTCTTCCTCCGTTACGACACGTTCTCCGTCGAGGTCAACGATTTGCCGTTGGGTCCGTCGGTGAAGATCACGTCCGTCACGAAAGCCGCCGTGAAGGTCTGA

Coding sequence (CDS)

ATGGCTTCCCCTTCTTCTTCCTCTGAACTCCCTCTGAAACCCATTCCCGGCGGCTATGGCTTCCCCTTCCTTGGAGCAATCAAAGACCGATACGATTACTTCTACTTCCAAGGGAAAGACGAATTCTTCCGCTCCCGAGTCGCCAAATACCGCTCCACCGTCTTCCGCGCCAACATGCCGCCGGGCCCCTTCATCTCCTCCGACTCCAGAGTCGTCGTCCTTCTCGACGCTCTCACCTTTCAGATCCTTTTTGATACTTCTAAGGTCGAGAAACGCAATCTTCTCGATGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGATATTCGTACCTGCGCCTATTTGGACCCGTCGGAAACAGAGCACTCTGTTTTGAAAAAGCTCTTCCTCTGTTTTCTTGCATCTCGTCACGACAGGTTTATCCCTCTGTTCCGAAGCTCCTTGTCGGAGATGTTTATTAAACTTGAAGATAAAATCGCTGAGAAGAATAAGGTCGTTGATTTCAACCCGATTAGTGATTCCATGTCGTTTGACTTCATATTCCGGCTGCTCTCCGACGGAACCCCTGACTCTAAACTGGCAGCCGATGGACCTGGAATGTTTGATAAATGGCTCGCTTTCCAGCTCGCCCCACTGGCTTCCATTGGTTTGCCGAAGATATTCTCAATTTTAGAAGATCTCGTCATTCACACCATGCCACTGCCTTTCTTTCTTGTCAAAAGTGGTTACATGAAGCTTTACAATGCGTTTTACTCCTGCTCTGATTCGTTTCTAGCCGAAGCGGAGAAACAGGGGATAAACAGAGAGAAAGCGTGTCACAATCTAGTGTTTCTGGCCGGATTCAATGCGTACGGCGGAATGAAAGTACTTTTTCCGATTTTGCTGAAGTGGATCGGCACCGCTGGGGAGGAGCTTCACCGGAATCTCGCCGACGAAATCAGAGCCGCCGTGAAGGAAGAAGGGGGATTGACATTTAGTGCAATAGAGAAGATGAGTCTATTGAAGTCCGTCGTGTACGAAGCGCTGAGGATCGAGCCGCCGGTGCCGTTCCAGTACGCGAAAGCGAAGGAGGACATCGTGGTACAGAGCCACGATTCCGCTTTCAAGATAAAGAAAGGGGAGATCATTTTTGGGTATCAGCCGTTTGCAACTAAAGATGGAAAGGTTTTTGAGAATCCGGAGAAGTTCATCGGCGACCGGTTCGTCGGAGAAGAAGGGGAGAAGCTTCTGAAGTATGTGTACTGGTCGAACGGTAGGGAGACAGAGGAGCCGACGCCGGAGGACAAGATGTGTCCGGGGAAAAATCTGGTGGTGCTGATGGCCAGAGTTATTGTAGTGGAATTCTTCCTCCGTTACGACACGTTCTCCGTCGAGGTCAACGATTTGCCGTTGGGTCCGTCGGTGAAGATCACGTCCGTCACGAAAGCCGCCGTGAAGGTCTGA

Protein sequence

MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPPGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGTAGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV
Homology
BLAST of CmoCh11G013280 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 4.5e-160
Identity = 276/474 (58.23%), Postives = 353/474 (74.47%), Query Frame = 0

Query: 5   SSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPPGPF 64
           SS   LP+K IPG YG PF GAIKDRYD+ Y QG DEFFRSR+ KY STVFR N+PPGPF
Sbjct: 18  SSIPSLPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRTNVPPGPF 77

Query: 65  ISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 124
            + +S+VVVL+DA+++ ILFD S+V+K N  +GT+M S SF G  + C +L  S+ +H+ 
Sbjct: 78  NARNSKVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGTSDPKHTT 137

Query: 125 LKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLL 184
           LK LFL  L   HD+FIP+F +S++ MF  LE +++EK     FNPI D++SF+F+FRL 
Sbjct: 138 LKGLFLSTLTRLHDKFIPIFTTSITSMFTSLEKELSEKGTSY-FNPIGDNLSFEFLFRLF 197

Query: 185 SDGTP--DSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSG 244
            +G    D+ +  +GP + DKW+  QLAPL S+GL  + + LEDLV+HT PLP+ LVK  
Sbjct: 198 CEGKNPIDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPYILVKRD 257

Query: 245 YMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGTAG 304
           + KLYNAFY+     L EAEK G+ R++ACHN VFLAGFN+YGG+KV FP L+KWIGT+G
Sbjct: 258 HQKLYNAFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIKWIGTSG 317

Query: 305 EELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQ 364
             LH  L  EIR AVKE GG+T SAI+KM L+KSVVYE LR++PPVPFQ  KA+++I++ 
Sbjct: 318 PSLHARLVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKARKNIIIT 377

Query: 365 SHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETE 424
           +H+S+F IKK E+IFGYQP ATKD KVF+N E+F  DRFVG  GEKLLKYVYWSNG+E +
Sbjct: 378 NHESSFLIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVG-GGEKLLKYVYWSNGKEID 437

Query: 425 EPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
            P+  DK CPGK+L+VLM R++VVEFF+RYDTF VE   L LG  V   S+TKA
Sbjct: 438 NPSVNDKQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKA 489

BLAST of CmoCh11G013280 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 5.2e-148
Identity = 263/484 (54.34%), Postives = 349/484 (72.11%), Query Frame = 0

Query: 3   SPSSSS-------ELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVF 62
           SPSSSS       +LP + +PG YG P +G  KDR DYFY QGK+EFF+SR+ K++STVF
Sbjct: 54  SPSSSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVF 113

Query: 63  RANMPPGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYL 122
           R NMPPGPFIS +  VVVLLD  +F +LFD SKVEK++L  GT+MPS   TG  R  +YL
Sbjct: 114 RTNMPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYL 173

Query: 123 DPSETEHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSM 182
           DPSE  H+ LKKL    L+SR +  IP F +S SE+F  LE++++ K K    N  +D  
Sbjct: 174 DPSEPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGK-AGLNAANDQA 233

Query: 183 SFDFIFRLLSDGTP-DSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPL 242
           +F+F+ R L    P D++L  DGP +  KW+ FQL PL  +GLPK   +LEDLV+HT  L
Sbjct: 234 AFNFLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPK---VLEDLVMHTFRL 293

Query: 243 PFFLVKSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPIL 302
           P  LVK  Y +LYN FY  S S L EAEK GI+RE+ACHNL+F   FN++GG+K+ FP +
Sbjct: 294 PPALVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNM 353

Query: 303 LKWIGTAGEELHRNLADEIRAAVKEEGG-LTFSAIEKMSLLKSVVYEALRIEPPVPFQYA 362
           LKWIG AG +LH  LA EIR+ +    G +T +A+EKM L+KSVVYE+LRIEPPV  QY 
Sbjct: 354 LKWIGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYG 413

Query: 363 KAKEDIVVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYV 422
           +AK D+V++SHD++F+IK+GE+++GYQPFATKD K+F+  E+F+ DRF+GEEGEKLLK+V
Sbjct: 414 RAKHDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHV 473

Query: 423 YWSNGRETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSV 478
            WSNG ETE  +  +K C GK+ VVL++R+++VE FLRYD+F +EV   PLG ++ +TS+
Sbjct: 474 LWSNGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSL 533

BLAST of CmoCh11G013280 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.7e-144
Identity = 253/470 (53.83%), Postives = 338/470 (71.91%), Query Frame = 0

Query: 9   ELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPPGPFISSD 68
           +L  + IPG YG P +G  KDR DYFY QGK++FF SR+AKY+ST+FR NMPPGPFI+S+
Sbjct: 45  KLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITSN 104

Query: 69  SRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSVLKKL 128
            +V+VLLD  +F +LFD SKVEK++L  GT++PS   TG  R  +YLDPSE  H  LKKL
Sbjct: 105 PKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKKL 164

Query: 129 FLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLLSDGT 188
               L+SR D  IP F  + +E+F  L+ ++ EK   V FN  SD  +F+F+ R L    
Sbjct: 165 MFFLLSSRRDHVIPEFHETYTELFETLDKEMEEKG-TVGFNSGSDQAAFNFLARSLFGVN 224

Query: 189 P-DSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYMKLY 248
           P ++KL  DGP +  KW+  QL P+ ++GLPK    L+D+++HT  LP  LVK  Y +LY
Sbjct: 225 PVETKLGTDGPALIGKWILLQLHPVITLGLPK---FLDDVLLHTFRLPPILVKKDYQRLY 284

Query: 249 NAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGTAGEELHR 308
           + FY+ S +   EAEK GI++++ACHNL+F   FN++GGMK+ FP +LK I  AG E+H 
Sbjct: 285 DFFYTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHT 344

Query: 309 NLADEIRAAVKEEGG-LTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSHDS 368
            LA+EIR+ VK  GG +T SA+EKM L+KSVVYEALR++PPV  QY +AK+D+ ++SHD+
Sbjct: 345 RLANEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDA 404

Query: 369 AFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEPTP 428
            F++KKGEI+FGYQPFATKD K+F+ P +F+ DRFVGEEGEKLLK+V WSNG ETE PT 
Sbjct: 405 VFEVKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTV 464

Query: 429 EDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
            +K C GK+ VV+++R+ V EFFLRY T +V+V    LG S+ ITS+ KA
Sbjct: 465 GNKQCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

BLAST of CmoCh11G013280 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 509.6 bits (1311), Expect = 3.8e-143
Identity = 252/479 (52.61%), Postives = 336/479 (70.15%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           +A+ + S +LP++ IPG YG P +G IKDR+DYFY QG +EFF+SR+ KY STV+R NMP
Sbjct: 43  VATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMP 102

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PG FI+ + +VV LLD  +F +LFD  KVEK++L  GTYMPS   TG  R  +YLDPSE 
Sbjct: 103 PGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEP 162

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           +H  LK L    L S  +R  P F+++ SE+F  LE +++ K K  DF   SD  +F+F+
Sbjct: 163 KHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFL 222

Query: 181 FRLLSDGTP-DSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLV 240
            R      P D+KL AD PG+  KW+ F L PL SIGLP+   ++E+ +IHT  LP  LV
Sbjct: 223 ARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALV 282

Query: 241 KSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIG 300
           KS Y +LY  F   +   L EA+K GI+RE+A HNL+F   FN +GGMK+LFP ++K IG
Sbjct: 283 KSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIG 342

Query: 301 TAGEELHRNLADEIRAAVKEEGG-LTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKED 360
            AG ++H  LA+EIR+ +K  GG LT  AIEKM L KSVVYE LR EPPV  QY +AK+D
Sbjct: 343 RAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKD 402

Query: 361 IVVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNG 420
           +V++SHD+AFK+K GE+++GYQP AT+D K+F+  ++F+ +RFVGEEGEKLL++V WSNG
Sbjct: 403 LVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNG 462

Query: 421 RETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAA 478
            ETE PT  +K C GK+ VVL+AR+ V+E F RYD+F +EV   PLG SV  +S+ KA+
Sbjct: 463 PETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

BLAST of CmoCh11G013280 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 6.8e-140
Identity = 248/478 (51.88%), Postives = 342/478 (71.55%), Query Frame = 0

Query: 4   PSSSSE--LPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPP 63
           P SS E  LP++ IPG YG P +G I+DR DYFY QG++EFF+SR+ KY+STV+RANMPP
Sbjct: 62  PPSSDETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKSTVYRANMPP 121

Query: 64  GPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETE 123
           GPFI+S+ RV+VLLDA +F +LFD SKVEK++L  GTYMPS   TG  R  +YLDPSE  
Sbjct: 122 GPFIASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPN 181

Query: 124 HSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIF 183
           H+ LK+L    + +R D  IP F SS +++   +E  +A K K   FN  ++  +F+F+ 
Sbjct: 182 HTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAA-FNDPAEQAAFNFLS 241

Query: 184 RLLSDGTP-DSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 243
           R      P D+ L  D P +  KW+ F LAP+ S+GLPK    +E+  +H++ LP  LV+
Sbjct: 242 RAFFGVKPIDTPLGKDAPSLISKWVLFNLAPILSVGLPK---EVEEATLHSVRLPPLLVQ 301

Query: 244 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 303
           + Y +LY  F S + S L EAE+ GI+R++ACHN++F   FN++GG K+LFP L+KWIG 
Sbjct: 302 NDYHRLYEFFTSAAGSVLDEAEQSGISRDEACHNILFAVCFNSWGGFKILFPSLMKWIGR 361

Query: 304 AGEELHRNLADEIRAAVKEEGG--LTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKED 363
           AG ELH  LA EIR+A++  GG  +T +A+E+M L+KSVVYE LRIEPPV  QY KAK+D
Sbjct: 362 AGLELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQYGKAKKD 421

Query: 364 IVVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNG 423
            +++SH++A+++K+GE++FGYQPFATKD K+F+ PE+F+ DRFVG EG KL++YV WSNG
Sbjct: 422 FILESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVG-EGVKLMEYVMWSNG 481

Query: 424 RETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
            ETE P+  +K C GK+ VV+ AR+ VVE F RYD+F +EV    LG S+ +TS+ ++
Sbjct: 482 PETETPSVANKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASITLTSLKRS 534

BLAST of CmoCh11G013280 vs. ExPASy TrEMBL
Match: A0A6J1EU14 (allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111437746 PE=4 SV=1)

HSP 1 Score: 959.1 bits (2478), Expect = 6.8e-276
Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP
Sbjct: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET
Sbjct: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI
Sbjct: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180

Query: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240
           FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK
Sbjct: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240

Query: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300
           SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT
Sbjct: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300

Query: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360
           AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV
Sbjct: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360

Query: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420
           VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE
Sbjct: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420

Query: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480
           TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV
Sbjct: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480

BLAST of CmoCh11G013280 vs. ExPASy TrEMBL
Match: A0A6J1JN86 (allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486003 PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.7e-271
Identity = 471/480 (98.12%), Postives = 474/480 (98.75%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRV KYRSTVFRANMP
Sbjct: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVTKYRSTVFRANMP 60

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET
Sbjct: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           EHSVLKK FLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI
Sbjct: 121 EHSVLKKHFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180

Query: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240
           FRLLSDGTPDSKLAADGPGMFDKWL FQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK
Sbjct: 181 FRLLSDGTPDSKLAADGPGMFDKWLTFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240

Query: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300
           SGY KLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKW+GT
Sbjct: 241 SGYRKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGT 300

Query: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360
           AGE+LHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV
Sbjct: 301 AGEKLHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360

Query: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420
           VQSHDSAFKIKKGE IFGYQPFATKDG+VFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE
Sbjct: 361 VQSHDSAFKIKKGETIFGYQPFATKDGRVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420

Query: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480
           TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAA KV
Sbjct: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAAKV 480

BLAST of CmoCh11G013280 vs. ExPASy TrEMBL
Match: Q93XR3 (Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 6.6e-239
Identity = 407/477 (85.32%), Postives = 444/477 (93.08%), Query Frame = 0

Query: 1   MASPSSSS-ELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANM 60
           MA+PSSSS ELPLKPIPGGYGFPFLG IKDRYDYFYFQG+DEFFRSR+ KY STVFRANM
Sbjct: 1   MATPSSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANM 60

Query: 61  PPGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSE 120
           PPGPFISSDSRVVVLLDAL+F ILFDT+KVEKRN+LDGTYMPSLSFTG+IRTCAYLDPSE
Sbjct: 61  PPGPFISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSE 120

Query: 121 TEHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDF 180
           TEHSVLK+LFL FLASRHDRFIPLFRSSLSEMF+KLEDK++EK K+ DFN ISDSMSFD+
Sbjct: 121 TEHSVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDY 180

Query: 181 IFRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLV 240
           +FRLLSDGTPDSKLAA+GPGMFD WL FQLAPLASIGLPKIFS+ EDLVIHT+PLPFF V
Sbjct: 181 VFRLLSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPV 240

Query: 241 KSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIG 300
           KSGY KLY AFYS S SFL EAEKQGI+REKACHNLVFLAGFNAYGGMKVLFP LLKW+G
Sbjct: 241 KSGYRKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVG 300

Query: 301 TAGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDI 360
           TAGE+LHR LA+E+R  VKEEGGLTFSA+EKMSLLKSVVYEALRIEPPVPFQY KAKEDI
Sbjct: 301 TAGEDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDI 360

Query: 361 VVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGR 420
           V+QSHDS+FKIKKGE IFGYQPFATKD K+F++ EKF+GDRFVGEEGEKLLKYVYWSN R
Sbjct: 361 VIQSHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNER 420

Query: 421 ETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
           ET EPTPE+K CPGKNLVVL+ R++VVEFFLRYDTF+VEV DLPLGP+VK  S+T+A
Sbjct: 421 ETVEPTPENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRA 477

BLAST of CmoCh11G013280 vs. ExPASy TrEMBL
Match: A0A5D3C5U2 (Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00110 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.6e-238
Identity = 406/477 (85.12%), Postives = 443/477 (92.87%), Query Frame = 0

Query: 1   MASPSSSS-ELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANM 60
           MA+PSSSS ELPLKPIPGGYGFPFLG IKDRYDYFYFQG+DEFFRSR+ KY STVFRANM
Sbjct: 1   MATPSSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANM 60

Query: 61  PPGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSE 120
           PPGPFISSDSRVVVLLDAL+F ILFDT+KVEKRN+LDGTYMPSLSFTG+IRTCAYLDPSE
Sbjct: 61  PPGPFISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSE 120

Query: 121 TEHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDF 180
           TEHSVLK+LFL FLASRHDRFIPLFRSSLSEMF+KLEDK++EK K+ DFN ISDSMSFD+
Sbjct: 121 TEHSVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDY 180

Query: 181 IFRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLV 240
           +FRLLSDGTPDSKLAA+GPGMFD WL FQLAPLASIGLPKIFS+ EDLVIHT+PLPFF V
Sbjct: 181 VFRLLSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPV 240

Query: 241 KSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIG 300
           KSGY KLY AFYS S SFL EAEKQGI+REKACHNLVFLAGFNAYGGMKVLFP LLKW+G
Sbjct: 241 KSGYRKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVG 300

Query: 301 TAGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDI 360
           TAGE+LHR LA+E+R  VKEEGGLTFSA+EKMSLLKSVVYEALRIEPPVPFQY KAKEDI
Sbjct: 301 TAGEDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDI 360

Query: 361 VVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGR 420
           V+QSHDS+FKIKKGE IFGYQPFATKD K+F++ EKF+GDRFVGEEGEKLLKYVYWSN R
Sbjct: 361 VIQSHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNER 420

Query: 421 ETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
           ET EPT E+K CPGKNLVVL+ R++VVEFFLRYDTF+VEV DLPLGP+VK  S+T+A
Sbjct: 421 ETVEPTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRA 477

BLAST of CmoCh11G013280 vs. ExPASy TrEMBL
Match: A0A1S3BZK9 (allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.6e-238
Identity = 406/477 (85.12%), Postives = 443/477 (92.87%), Query Frame = 0

Query: 1   MASPSSSS-ELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANM 60
           MA+PSSSS ELPLKPIPGGYGFPFLG IKDRYDYFYFQG+DEFFRSR+ KY STVFRANM
Sbjct: 1   MATPSSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANM 60

Query: 61  PPGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSE 120
           PPGPFISSDSRVVVLLDAL+F ILFDT+KVEKRN+LDGTYMPSLSFTG+IRTCAYLDPSE
Sbjct: 61  PPGPFISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSE 120

Query: 121 TEHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDF 180
           TEHSVLK+LFL FLASRHDRFIPLFRSSLSEMF+KLEDK++EK K+ DFN ISDSMSFD+
Sbjct: 121 TEHSVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDY 180

Query: 181 IFRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLV 240
           +FRLLSDGTPDSKLAA+GPGMFD WL FQLAPLASIGLPKIFS+ EDLVIHT+PLPFF V
Sbjct: 181 VFRLLSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPV 240

Query: 241 KSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIG 300
           KSGY KLY AFYS S SFL EAEKQGI+REKACHNLVFLAGFNAYGGMKVLFP LLKW+G
Sbjct: 241 KSGYRKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVG 300

Query: 301 TAGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDI 360
           TAGE+LHR LA+E+R  VKEEGGLTFSA+EKMSLLKSVVYEALRIEPPVPFQY KAKEDI
Sbjct: 301 TAGEDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDI 360

Query: 361 VVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGR 420
           V+QSHDS+FKIKKGE IFGYQPFATKD K+F++ EKF+GDRFVGEEGEKLLKYVYWSN R
Sbjct: 361 VIQSHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNER 420

Query: 421 ETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
           ET EPT E+K CPGKNLVVL+ R++VVEFFLRYDTF+VEV DLPLGP+VK  S+T+A
Sbjct: 421 ETVEPTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRA 477

BLAST of CmoCh11G013280 vs. NCBI nr
Match: XP_022931572.1 (allene oxide synthase 3-like [Cucurbita moschata])

HSP 1 Score: 959.1 bits (2478), Expect = 1.4e-275
Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP
Sbjct: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET
Sbjct: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI
Sbjct: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180

Query: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240
           FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK
Sbjct: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240

Query: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300
           SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT
Sbjct: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300

Query: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360
           AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV
Sbjct: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360

Query: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420
           VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE
Sbjct: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420

Query: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480
           TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV
Sbjct: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480

BLAST of CmoCh11G013280 vs. NCBI nr
Match: XP_023530097.1 (allene oxide synthase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 952.2 bits (2460), Expect = 1.7e-273
Identity = 476/480 (99.17%), Postives = 478/480 (99.58%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           MASP+SSSELPLK IPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP
Sbjct: 1   MASPTSSSELPLKSIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET
Sbjct: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI
Sbjct: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180

Query: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240
           FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK
Sbjct: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240

Query: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300
           SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKW+GT
Sbjct: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGT 300

Query: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360
           AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV
Sbjct: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360

Query: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420
           VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE
Sbjct: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420

Query: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480
           TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAV V
Sbjct: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVTV 480

BLAST of CmoCh11G013280 vs. NCBI nr
Match: KAG6588631.1 (Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022428.1 Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 949.5 bits (2453), Expect = 1.1e-272
Identity = 477/480 (99.38%), Postives = 478/480 (99.58%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           MASP SSSELPLK IPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP
Sbjct: 1   MASP-SSSELPLKSIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET
Sbjct: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI
Sbjct: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180

Query: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240
           FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK
Sbjct: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240

Query: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300
           SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKW+GT
Sbjct: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGT 300

Query: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360
           AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV
Sbjct: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360

Query: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420
           VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE
Sbjct: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420

Query: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480
           TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV
Sbjct: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 479

BLAST of CmoCh11G013280 vs. NCBI nr
Match: XP_022988758.1 (allene oxide synthase 3-like [Cucurbita maxima] >XP_022988759.1 allene oxide synthase 3-like [Cucurbita maxima])

HSP 1 Score: 944.5 bits (2440), Expect = 3.6e-271
Identity = 471/480 (98.12%), Postives = 474/480 (98.75%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRV KYRSTVFRANMP
Sbjct: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVTKYRSTVFRANMP 60

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET
Sbjct: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           EHSVLKK FLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI
Sbjct: 121 EHSVLKKHFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180

Query: 181 FRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240
           FRLLSDGTPDSKLAADGPGMFDKWL FQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK
Sbjct: 181 FRLLSDGTPDSKLAADGPGMFDKWLTFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVK 240

Query: 241 SGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGT 300
           SGY KLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKW+GT
Sbjct: 241 SGYRKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGT 300

Query: 301 AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360
           AGE+LHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV
Sbjct: 301 AGEKLHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIV 360

Query: 361 VQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420
           VQSHDSAFKIKKGE IFGYQPFATKDG+VFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE
Sbjct: 361 VQSHDSAFKIKKGETIFGYQPFATKDGRVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRE 420

Query: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480
           TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAA KV
Sbjct: 421 TEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAAKV 480

BLAST of CmoCh11G013280 vs. NCBI nr
Match: NP_001284390.1 (allene oxide synthase 2-like [Cucumis melo] >AAK54282.1 fatty acid 9-hydroperoxide lyase [Cucumis melo])

HSP 1 Score: 836.3 bits (2159), Expect = 1.4e-238
Identity = 407/477 (85.32%), Postives = 444/477 (93.08%), Query Frame = 0

Query: 1   MASPSSSS-ELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANM 60
           MA+PSSSS ELPLKPIPGGYGFPFLG IKDRYDYFYFQG+DEFFRSR+ KY STVFRANM
Sbjct: 1   MATPSSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANM 60

Query: 61  PPGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSE 120
           PPGPFISSDSRVVVLLDAL+F ILFDT+KVEKRN+LDGTYMPSLSFTG+IRTCAYLDPSE
Sbjct: 61  PPGPFISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSE 120

Query: 121 TEHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDF 180
           TEHSVLK+LFL FLASRHDRFIPLFRSSLSEMF+KLEDK++EK K+ DFN ISDSMSFD+
Sbjct: 121 TEHSVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDY 180

Query: 181 IFRLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLV 240
           +FRLLSDGTPDSKLAA+GPGMFD WL FQLAPLASIGLPKIFS+ EDLVIHT+PLPFF V
Sbjct: 181 VFRLLSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPV 240

Query: 241 KSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIG 300
           KSGY KLY AFYS S SFL EAEKQGI+REKACHNLVFLAGFNAYGGMKVLFP LLKW+G
Sbjct: 241 KSGYRKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVG 300

Query: 301 TAGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDI 360
           TAGE+LHR LA+E+R  VKEEGGLTFSA+EKMSLLKSVVYEALRIEPPVPFQY KAKEDI
Sbjct: 301 TAGEDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDI 360

Query: 361 VVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGR 420
           V+QSHDS+FKIKKGE IFGYQPFATKD K+F++ EKF+GDRFVGEEGEKLLKYVYWSN R
Sbjct: 361 VIQSHDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNER 420

Query: 421 ETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
           ET EPTPE+K CPGKNLVVL+ R++VVEFFLRYDTF+VEV DLPLGP+VK  S+T+A
Sbjct: 421 ETVEPTPENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRA 477

BLAST of CmoCh11G013280 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 509.6 bits (1311), Expect = 2.7e-144
Identity = 252/479 (52.61%), Postives = 336/479 (70.15%), Query Frame = 0

Query: 1   MASPSSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMP 60
           +A+ + S +LP++ IPG YG P +G IKDR+DYFY QG +EFF+SR+ KY STV+R NMP
Sbjct: 43  VATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMP 102

Query: 61  PGPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSET 120
           PG FI+ + +VV LLD  +F +LFD  KVEK++L  GTYMPS   TG  R  +YLDPSE 
Sbjct: 103 PGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEP 162

Query: 121 EHSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFI 180
           +H  LK L    L S  +R  P F+++ SE+F  LE +++ K K  DF   SD  +F+F+
Sbjct: 163 KHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFL 222

Query: 181 FRLLSDGTP-DSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLV 240
            R      P D+KL AD PG+  KW+ F L PL SIGLP+   ++E+ +IHT  LP  LV
Sbjct: 223 ARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALV 282

Query: 241 KSGYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIG 300
           KS Y +LY  F   +   L EA+K GI+RE+A HNL+F   FN +GGMK+LFP ++K IG
Sbjct: 283 KSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIG 342

Query: 301 TAGEELHRNLADEIRAAVKEEGG-LTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKED 360
            AG ++H  LA+EIR+ +K  GG LT  AIEKM L KSVVYE LR EPPV  QY +AK+D
Sbjct: 343 RAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKD 402

Query: 361 IVVQSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNG 420
           +V++SHD+AFK+K GE+++GYQP AT+D K+F+  ++F+ +RFVGEEGEKLL++V WSNG
Sbjct: 403 LVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNG 462

Query: 421 RETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAA 478
            ETE PT  +K C GK+ VVL+AR+ V+E F RYD+F +EV   PLG SV  +S+ KA+
Sbjct: 463 PETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

BLAST of CmoCh11G013280 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 263.8 bits (673), Expect = 2.6e-70
Identity = 144/391 (36.83%), Postives = 219/391 (56.01%), Query Frame = 0

Query: 89  VEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSVLKKLFLCFLASRHDRFIPLFRSSL 148
           V+KR++L G + PSL F G +R   YLD +E +H+ +K   +  L      ++   RS+L
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 149 SEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLLS--DGTPDSKLAADGPGMFDKWLA 208
           +  +  +E +I++        P+   + F F+   L+  D +    +A +G    + WLA
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCI-FSFLCASLAGVDASVSPDIAENGWKTINTWLA 123

Query: 209 FQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYMKLYNAF-YSCSDSFLAEAEKQG 268
            Q+ P A +G+  +   LE++++HT P P  L+   Y KLYN    +  D      E+ G
Sbjct: 124 LQVIPTAKLGV--VPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 183

Query: 269 INREKACHNLVFLAGFNAYGGMKVLFPILLKWIGTAGEELHRNLADEIRAAVKEEGGLTF 328
           + R++A  NL+F+ GFNAYGG  V  P L+  I      L   +  E+R        L F
Sbjct: 184 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 243

Query: 329 SAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSHDSAFKIKKGEIIFGYQPFATK 388
             + +M L+KSVVYE LR  PPVP Q+A+A++D  + SHD+ F++KKGE++ GYQP   +
Sbjct: 244 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 303

Query: 389 DGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEPTPEDKMCPGKNLVVLMARVIV 448
           D  VF+ PE+F  DR+VGE G +LL Y+YWSNG +T  P+  +K C  K++V L A ++V
Sbjct: 304 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 363

Query: 449 VEFFLRYDTFSVEVNDLPLGPSVKITSVTKA 477
            + FLRYDT +        G S  I +V KA
Sbjct: 364 ADLFLRYDTIT--------GDSGSIKAVVKA 383

BLAST of CmoCh11G013280 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 58.5 bits (140), Expect = 1.7e-08
Identity = 60/201 (29.85%), Postives = 90/201 (44.78%), Query Frame = 0

Query: 294 LLKWIG---TAGEELHRNLADEIRAAVKEEGGLTFSA-IEKMSLLKSVVYEALRIEPP-- 353
           +L+WI       +E+   L +EI   V EE  +      +KM  LK+VV EALR  PP  
Sbjct: 319 VLQWIMANLVKNQEIQERLYEEITNVVGEEAKVVEEKDTQKMPYLKAVVMEALRRHPPGN 378

Query: 354 --VPFQYAKAKEDIVVQSHDSAFKI-KKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGE 413
             +P       ED V+      +K+ KKG I F       +D KV+E P  F  +RF+GE
Sbjct: 379 TVLPH---SVTEDTVL----GGYKVPKKGTINFLVAEIG-RDPKVWEEPMAFKPERFMGE 438

Query: 414 E------GEKLLKYVYWSNGRETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVE 473
           E      G + +K + +  GR         ++CPG  L +L     V      +    VE
Sbjct: 439 EEAVDITGSRGIKMMPFGAGR---------RICPGIGLAMLHLEYYVANMVREFQWKEVE 498

Query: 474 VNDLPLGPSVKITSVTKAAVK 480
            +++ L   V+ T + K  +K
Sbjct: 499 GHEVDLTEKVEFTVIMKHPLK 502

BLAST of CmoCh11G013280 vs. TAIR 10
Match: AT5G42590.1 (cytochrome P450, family 71, subfamily A, polypeptide 16 )

HSP 1 Score: 57.8 bits (138), Expect = 2.8e-08
Identity = 49/198 (24.75%), Postives = 85/198 (42.93%), Query Frame = 0

Query: 281 FNAYGGMKVLFPILLKWIGT---AGEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSV 340
           F  + G       +++W  T      E  + L DEIR+  K    ++   +E M+ LK+V
Sbjct: 294 FEMFLGSTTTTSAVIEWAMTRLMRNPECLKKLQDEIRSVSKMNSYVSGKEVENMNYLKAV 353

Query: 341 VYEALRIEPPVPFQYAK-AKEDIVVQSHD---------SAFKIKKGEIIFGYQPFATKDG 400
           + E LR+ PP+P    +   ED+ ++ +D         +A+ I++    +G      +  
Sbjct: 354 IKEVLRLHPPLPLLVPRLLSEDVKLKGYDITAGTQVIINAWAIQRDTATWGSDAQEFRPE 413

Query: 401 KVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEPTPEDKMCPGKNLVVLMARVIVVE 460
           + F++   F+G  F         KY+ +  GR         ++CPG  L  +MA V +  
Sbjct: 414 RHFDSTWDFVGRNF---------KYIPFGAGR---------RLCPGIGLGSVMASVTLAN 470

Query: 461 FFLRYDTFSVEVNDLPLG 466
              R+D     V D P G
Sbjct: 474 LVKRFDW---RVEDGPSG 470

BLAST of CmoCh11G013280 vs. TAIR 10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 3.7e-08
Identity = 57/197 (28.93%), Postives = 89/197 (45.18%), Query Frame = 0

Query: 295 LKWIG---TAGEELHRNLADEIRAAVKEEG-GLTFSAIEKMSLLKSVVYEALRIEPPVPF 354
           L+WI        E+ + L +EI++ V EE   +     +KM  LK+VV E LR  PP  F
Sbjct: 321 LQWIMANLVKNPEIQKRLYEEIKSVVGEEAKEVEEEDAQKMPYLKAVVMEGLRRHPPGHF 380

Query: 355 QYA-KAKEDIVVQSHDSAFKI-KKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEE--- 414
                  ED V+      +K+ KKG I F       +D  V+E P  F  +RF+GEE   
Sbjct: 381 VLPHSVTEDTVL----GGYKVPKKGTINFMVAEIG-RDPMVWEEPMAFKPERFMGEEEAV 440

Query: 415 ---GEKLLKYVYWSNGRETEEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDL 474
              G + +K + +  GR         ++CPG  L +L     V      ++   V+ +++
Sbjct: 441 DITGSRGIKMMPFGAGR---------RICPGIGLAMLHLEYYVANMVREFEWKEVQGHEV 500

Query: 475 PLGPSVKITSVTKAAVK 480
            L    + T V K ++K
Sbjct: 501 DLTEKFEFTVVMKHSLK 503

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GZP54.5e-16058.23Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1[more]
K4BV525.2e-14854.34Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q9LLB02.7e-14453.83Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
Q962423.8e-14352.61Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
P484176.8e-14051.88Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE... [more]
Match NameE-valueIdentityDescription
A0A6J1EU146.8e-276100.00allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111437746 PE=4 ... [more]
A0A6J1JN861.7e-27198.13allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486003 PE=4 SV... [more]
Q93XR36.6e-23985.32Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1[more]
A0A5D3C5U25.6e-23885.12Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BZK95.6e-23885.12allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022931572.11.4e-275100.00allene oxide synthase 3-like [Cucurbita moschata][more]
XP_023530097.11.7e-27399.17allene oxide synthase 3-like [Cucurbita pepo subsp. pepo][more]
KAG6588631.11.1e-27299.38Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG702... [more]
XP_022988758.13.6e-27198.13allene oxide synthase 3-like [Cucurbita maxima] >XP_022988759.1 allene oxide syn... [more]
NP_001284390.11.4e-23885.32allene oxide synthase 2-like [Cucumis melo] >AAK54282.1 fatty acid 9-hydroperoxi... [more]
Match NameE-valueIdentityDescription
AT5G42650.12.7e-14452.61allene oxide synthase [more]
AT4G15440.12.6e-7036.83hydroperoxide lyase 1 [more]
AT1G64930.11.7e-0829.85cytochrome P450, family 87, subfamily A, polypeptide 7 [more]
AT5G42590.12.8e-0824.75cytochrome P450, family 71, subfamily A, polypeptide 16 [more]
AT2G12190.13.7e-0828.93Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 368..382
score: 18.72
coord: 331..347
score: 37.78
coord: 384..402
score: 27.53
coord: 431..449
score: 22.06
IPR001128Cytochrome P450PFAMPF00067p450coord: 305..453
e-value: 6.2E-16
score: 58.2
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 10..480
e-value: 5.5E-110
score: 370.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 11..470
NoneNo IPR availablePANTHERPTHR24286:SF302HYDROPEROXIDE LYASEcoord: 9..457
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 9..457

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh11G013280.1CmoCh11G013280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen