CmoCh11G010400 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh11G010400
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Calcium-binding EF hand family protein
Location	Cmo_Chr11: 5722455 .. 5727506 (+)
RNA-Seq Expression	CmoCh11G010400
Synteny	CmoCh11G010400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCTGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTCGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCAAAAGAAAAGGCTTCCCCGCAAGGAAATTCCTTAGAATCTGAGGCCACCCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGATGAGGAAATCGAGGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCGTGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATTTGATCATAACAAAGATGGTAAAATTGATTTCAATGAGTTTGTTAAGTTTATGGAAATTGCCTTGTCTTGAATTTTCAAATATGAACATAGGTGTTTCAATTTCCATTTCCATTTCCATTGTCATGTCCGACACTTTCATCGCCCTTTGATGTCTAGGGGTTCTAATGTGTCGACATAAGAGATAAATTAATGTTTTCTATAACATTAAAAAGATGTGCAAAGTATGAGAAAAGTCAAAAAGATGTCACCAATGTCAACATAACATTAAAAATAAATTTTAATTATTGATATGGAACATTTTATCGATGTTTTTAGTAAAGTAATAATTCTTCATGCATTCACATTTTATCTTGACCTGTACAAGTCATTAGTTGTTTGGAGTTTTAAAACAGACTTGACCTTGACCTTGAGGAAGAACGTGGTCTTCGAGCAGTTCAATTTTATTAGACGAGACGTATTTGAGCTCTGGGTGAACTAACTTTTTTGTTCTTTGGACTTCGTTAGCTTCCTTTTATCTTGTAGTGAAGGATTGATATCCTAGTTTGGAGGTGGGATCTCGTCGTATATCATAGAATAGTATGATGTCATCTCTTTAAGCATATATGTATAATGTTAATAGATGACCCTTCTATAAAAAACTACAAAGTCAAAAAGTTTGGGGGAGTGACTACATGTGTCACTCGGCTACTGAGTCTACTTATGAGGATTATTAGCAAGTTGTAACGCTCCATGCTTAGGATTTAAAATCTGGATTCGGTATTTAATGACCCTGACATTCCCCTACATCTTCAATGACATGGTCACGTTACTTCTCGTTTCTAAGAGTGAAGACTATCCTCATAGATCAACACTGAAGTTCCTATGACTGAAGCCACTGAAAAGGTAAGTATATCTCGTTGGTATAGATAGTAATTTTAAATTCTTTAAATATTTCTTAGTCATCCTACTCTTAGGGTCTCTCTCATTCGAATGTGATTTCTATCCATTTATATACCTCTCATTTACTTCAGCGCCACATGCCTACATGTTTCCATCTTGGTTCGTCTCGAACCACATTTTACTAAGAGAGGTACTTCTCTAATACCATTTGTAATGGCCCACGCCCACTGCATTCTCTGCGACATGGTTAAATTACTTTTAGTTTTTAAGAGTGAAAACTATCCCTATAGATTTATATGAGTCGTTTCAGCATGTTTTATGCTAGCTCACATGATTCTCACGAAAATTTCCTGGAAGTCACCTAACTTAAAATTGCTCTAAGCAAAATACACTTAACTTTTGAGTTCTCATGATTGAGACACTGAAAAAGAAGGTATCCCATGACTATTGCATGCAAGTTAATCATTCACTCGTTAACTATAGTTTGATCAAAAGTTTGTTTCCTTTGTAATAATTTTGTACCTTTAAGTAGTACTCATCTTCTAATGACCAATTTATTTATGATTGTATAGCTACCATATTTTCCTATTATAAATAAGGCCGCTACGGCATACCTGACATGTCTAGTATGCGTGAAATTTTTTATTTAAAATATTTCATACACAATATTATGAACTTAAACGTTCCTAAAATCTATTTTAAATAAAACTAAACATCTATGGAATAACAAAAACAAAATAACATAATTTAACGAGCAAGTCATCAAATCAAGTCAACAGCAAAACCACCCACTTACTTAATTGGCCTAAACCGAAGTCAGTGTATTCCTAGTTCTAGCTTAAAGTTGCTCCTTAAATTCTAATTCTATCTAAAGAGTCCCAAAATCATAATTTAGAATTCAATTATAGAAATGGACTCCCATTACCTAAAATAATCAAAACTTAGCTCTAAATACTTTAAATTAGATAAAAGTAGAACCTTATATTTTTTTTAAAAATCTATATATATCATATAATTTTAGTTTTAAAAAAACAGAAAATTTATGGCCAAACAAACCAAGAATGGAAGCTAAAATGTTTATAAAATTTATTATTTAAATACTTTCATCAAGGGATTCAAATAATTTCGAAGTTTAATTTTTAGAACTTAAAGTCAAATCTATGAATTTTTTTTCACAACATAAGGGATAATTTTATATAATTAATATAGAGAGTCACAATCTCATGCCAATTTTTGTTATTTTAGTTTTCCACGTCAAAATGTCAAATTGTCAAGATGACTGAGTGGTCTGACATTTTTATGTAGGTTTCTTTTATATAACAAATAAAAAGTGCTTTTACCTTACCTACTCTTAAAAACAATTATTCTAAAATTTTTAGGCATGGATGATAGTTTAGATTTGTTAAAAGTTCGATGTTCTTATTGTAAATCCGGAGTAAACAAGTGGCATATGAGTGAATCATGACCATATTTGAATGGAAGCGGATGTTGTGTTGAATTCATCTAGATTTTGAATCCTAGACATAGTGTGTTACCGTTATCATCTTAAAAAAAAAAAAAAATGATTCAGTTTTTTTTTTCTTAAGCTAGAATAAAAAAAAAAACACGTGAGGGGTAGAAGGTTTGTACTTAGATGATGTGAGCTGAAATATCCCACTCGTGTCTCCATAGCCATATGTTGTCCCCTGTCACAACATTTGGCCACATTTCACCCAATCCTGGTTGGACTATGTTCCCCTGACCAATTATGATCGCTGATGTAAAAGCATGACATACCAAAGAATTTGAATTGAAAAATAAGATCTGCTTCACATCAAAGGGGCTGCCAACACATTGTGTAGCGCCGCCATTGTATAGGTTTGGCCACACACCATGGATACGGAATCTATTACTTGGGTCTTGATACCATTCTGGCTTATTCTGATCACTACATGCTGTTGGTTGCCATTGAATTACGACTGGCAAGTAGTCATAGTTGTTGTTAAGGATATTTAGTAATAAATTATAGTTTACCATATATATTATATTTGATATTTTATTATAAGGCAAATATCTAATATTTGCCTTATTACCATCAGTATCTTTCAATTTATTGTTATTACCATAAGTATCTTTCCATTCATTGTTATTTCCATTTATTACTGTTTCTATATCATTGTAATTTATTTGATTATAAATAAGATAACTTTCACACCATTTAGGTGTGGTGGGTTAATCAAACATTCTCAGTTGTTATCTCCATCACAACTCACTATTAACATCAGTATCACAACTCACTATTAACATCCGTCACTCTTTGATATTGAGGTTGATATCAAATGCTTGTGAGTGATATAATAGAAGGAGGCTATTTATAGTAAGTCAATGTGATTGCTCCTCACTATCTTCCAAATGGCTGCTATCAGAGTTACTAAATTAAAATAATAAATAAAGTTTTTAAAGAAATTATCTTATTAGCATCTGTCACTCCATTTTAACCTTGTAAATATTGACTTTCTACAAAATTCTTCTTTGATCAGAGAGGTAAATATTGACTTTCTACAAGATTCTTCTTTGATTAGAGAGAGAAATGAGTGTCAGGAAAGACGGAAAGACGCTGACCTCGAAGGGGTAGATTGTGAGATCCCACATCGATTAAAGAGGGGAACGAAACATTATTTATGAGGGTGTGGAAATCTCTTCCTAGCCGACACGTTTTAAAAACTTTGAGGGAAAGCCTGAAATGACAAACTAAAAGAGTACAATGAAAAACTGTACTCTCTCAACACTAAGAATAGAGAAGAAAAGGTGTACTCTCTAAACATGAAGAATAGAGAAGAAAAGGTGCTCTCTCTAAACATGAAGAATAGCCACGAGTGGAAGATTTTGACAAAAGTAGAAGGTAACTTCCTACAAGAACCCAAAACACTACCATTTTTAGATTACCTACAAGAGCCCATTTCTCGTATTATAACCATCTCTTGGAATGATTCTTTGAAACAACCAACAATAATCATCCAAAATAACCCAAAACTGTCCCATTTTTAGCATTGCATTAGAACGTTTCAAACCGCAGCCTATATTCCCAAACTCCCTTCTAATGCATACATCCACTACAAATAACCACACTCTCAAAATCCTCTCTCCCACATTAGATTAACAAAGGCGTTGCATCGGCCTGCGTTAATTCTCCCACATTAGATTAACAATGGCGATGCGTCGGCCTATGTTATTGCTAGTGCTTGTGCTCATAGCCTTCTCTCACTTTTCGATGCTCACAACCTCGAAAAACAATAATAAGGTCCCAAAACCCAAAAAGACAAAGCCCAAATTTCTAAAACCAAACAATCCTAATCCCGCCTTTGAGCGTTTTACTGTAGAGATCCATAACGACTTGCGTATGTTCATATTGGATGCCCACTGCTCCTCGAAAGATGATGATTTAGGGTTACATATATTGTTCCCAGATGAGGAACAAGATTGGTCATTTCATGATAATTGGTTAGGCACTACCGAATTTCATTGTAGATTGGAGTGGCAATATGGGCTTTTAGAATTTGATGCCTTTCATAGCAACACTGATAATCTTTTGATCGATTATTGCGCCAACGCAACCTGCATTTGGAGTGCGAGACAAGATGGGGTTTATTTGAACAATCAAGATGACGATCTTGTTTTTTATGAGTATTGGGATATGCTTGGATAG

mRNA sequence

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCTGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTCGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCAAAAGAAAAGGCTTCCCCGCAAGGAAATTCCTTAGAATCTGAGGCCACCCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGATGAGGAAATCGAGGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCGTGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATTTGATCATAACAAAGATGGCACTACCGAATTTCATTGTAGATTGGAGTGGCAATATGGGCTTTTAGAATTTGATGCCTTTCATAGCAACACTGATAATCTTTTGATCGATTATTGCGCCAACGCAACCTGCATTTGGAGTGCGAGACAAGATGGGGTTTATTTGAACAATCAAGATGACGATCTTGTTTTTTATGAGTATTGGGATATGCTTGGATAG

Coding sequence (CDS)

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCTGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTCGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCAAAAGAAAAGGCTTCCCCGCAAGGAAATTCCTTAGAATCTGAGGCCACCCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGATGAGGAAATCGAGGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCGTGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATTTGATCATAACAAAGATGGCACTACCGAATTTCATTGTAGATTGGAGTGGCAATATGGGCTTTTAGAATTTGATGCCTTTCATAGCAACACTGATAATCTTTTGATCGATTATTGCGCCAACGCAACCTGCATTTGGAGTGCGAGACAAGATGGGGTTTATTTGAACAATCAAGATGACGATCTTGTTTTTTATGAGTATTGGGATATGCTTGGATAG

Protein sequence

MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNSLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFHCRLEWQYGLLEFDAFHSNTDNLLIDYCANATCIWSARQDGVYLNNQDDDLVFYEYWDMLG

Homology

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BLAST of CmoCh11G010400 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 7.1e-18
Identity = 47/102 (46.08%), Postives = 69/102 (67.65%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGC 132
           IS+EE + VM  L  F +D++++E +   +E+ ++F E E SLEE+KQ F+VFD+N+DG 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 133 IDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           IDA ELQ VL +L   QG ++ +C  MI   D NKDG  +F+
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFN 183

[Close]

BLAST of CmoCh11G010400 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.9e-15
Identity = 49/119 (41.18%), Postives = 71/119 (59.66%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSDEEIE--ESWLGGEEIGAIFYESEPSLEELKQTFNVFDK 129
           +  + +E+V  VM+ L    +D+E E  +     +E+  +F E EPSLEE+KQ F+VFD+
Sbjct: 88  DDGLCREDVGMVMKSLG-LSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDE 147

Query: 130 NRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFHCRLEWQYGLLEF 187
           NRDG ID  +LQ VL +L   QG  + +C RMI  FD +KDG  +F       YG ++F
Sbjct: 148 NRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDF-------YGFVKF 198

[Close]

BLAST of CmoCh11G010400 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.9e-10
Identity = 42/117 (35.90%), Postives = 64/117 (54.70%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSDEE----IEESWLGG------EEIGAIF---YESEPSLE 129
           +  I+K+E+   +E L  +  D++    IE+  L G      EE G ++    E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 130 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEF 174
           ++++ FNVFD+NRDG I  +EL+SVL  L   QG  + DC RMI++ D + DG   F
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNF 134

[Close]

BLAST of CmoCh11G010400 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.3e-08
Identity = 30/73 (41.10%), Postives = 42/73 (57.53%), Query Frame = 0


Query: 101 GEEIGAIFYESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMI 160
           GE    I  E +   E++K+ FNVFD+N DG I  DEL++VL  L   QG  + DC +MI
Sbjct: 63  GELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMI 122

Query: 161 ARFDHNKDGTTEF 174
            + D + DG   +
Sbjct: 123 KKVDVDGDGRVNY 135

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BLAST of CmoCh11G010400 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.5e-07
Identity = 39/116 (33.62%), Postives = 64/116 (55.17%), Query Frame = 0


Query: 63  SEATQHKNSSISKEEVKFVMEKLEF---FCSDEEIEESWLGGEEIGAIFYESEPSL--EE 122
           +E+ ++    I ++E+  +++K++     C D  IEE    GE    I  E E  +  E+
Sbjct: 27  NESFKNLGIIIPEDELTQIIQKIDVNGDGCVD--IEEF---GELYKTIMVEDEDEVGEED 86

Query: 123 LKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEF 174
           +K+ FNVFD+N DG I  DEL++VL  L   QG  + +C +MI + D + DG   +
Sbjct: 87  MKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNY 137

[Close]

BLAST of CmoCh11G010400 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.2e-89
Identity = 170/174 (97.70%), Postives = 172/174 (98.85%), Query Frame = 0


Query: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDG  +F+
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFN 174

[Close]

BLAST of CmoCh11G010400 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 3.0e-88
Identity = 167/174 (95.98%), Postives = 170/174 (97.70%), Query Frame = 0


Query: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFP+VNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEK SPQGNS
Sbjct: 1   MPSQFPIVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKVSPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDG  +F+
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFN 174

[Close]

BLAST of CmoCh11G010400 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.2e-36
Identity = 95/180 (52.78%), Postives = 122/180 (67.78%), Query Frame = 0


Query: 6   PVVNLKLALT--------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQ 65
           P +NL+L  +        L+ +DR SI      F D IHDL+S  +S F SPPKEKA+PQ
Sbjct: 6   PTINLQLIPSTTTMMPPPLVFIDRASI------FADTIHDLVSLAFSFFQSPPKEKAAPQ 65

Query: 66  GNSLESEATQHKNSSISKEEVKFVMEKLEFFCS---DEEIEESWLGGEEIGAIFYESEPS 125
             + +SE  +  +  +S+EEVK VMEKLE +C+   D+EI+E + G EE+  +F E+EPS
Sbjct: 66  SRASQSEGKE--DWLMSREEVKSVMEKLELYCNEEGDDEIDECF-GREEVRGMFEENEPS 125

Query: 126 LEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           LEELKQTFNVFD+NRDG IDA EL +VLGLLV N  +FIHDC  MIARFD N D   +F+
Sbjct: 126 LEELKQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFN 176

[Close]

BLAST of CmoCh11G010400 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 2.3e-35
Identity = 90/178 (50.56%), Postives = 119/178 (66.85%), Query Frame = 0


Query: 6   PVVNLKLALT-------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQG 65
           P +NL+L  +       L+ +DR SI      F   IHDL+S  +S F SPPK+KA+PQ 
Sbjct: 6   PTINLQLIPSTTTTTPPLVFIDRASI------FAGTIHDLVSLAFSFFQSPPKDKAAPQS 65

Query: 66  NSLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEE--SWLGGEEIGAIFYESEPSLE 125
            + +SE+ +  +  +S+EEVK VMEKLE +C++EE +E     G +E+  +F E+EPSLE
Sbjct: 66  RASQSESKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLE 125

Query: 126 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           ELKQTFNVFD+NRDG IDA EL +VLGLL  N  +FIHDC  MIARFD N D   +F+
Sbjct: 126 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFN 175

[Close]

BLAST of CmoCh11G010400 vs. ExPASy TrEMBL
Match: A0A6J1F5H5 (S-protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111441018 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 9.6e-34
Identity = 68/72 (94.44%), Postives = 68/72 (94.44%), Query Frame = 0


Query: 163 FDHNKDGTTEFHCRLEWQYGLLEFDAFHSNTDNLLIDYCANATCIWSARQDGVYLNNQDD 222
           F  N  GTTEFHCRLEWQYGLLEFDAFHSNTDNLLIDYCANATCIWSARQDGVYLNNQDD
Sbjct: 94  FHDNWLGTTEFHCRLEWQYGLLEFDAFHSNTDNLLIDYCANATCIWSARQDGVYLNNQDD 153

Query: 223 DLVFYEYWDMLG 235
           DLVFYEYWDMLG
Sbjct: 154 DLVFYEYWDMLG 165

[Close]

BLAST of CmoCh11G010400 vs. NCBI nr
Match: XP_022927644.1 (probable calcium-binding protein CML30 [Cucurbita moschata])

HSP 1 Score: 339.3 bits (869), Expect = 2.6e-89
Identity = 170/174 (97.70%), Postives = 172/174 (98.85%), Query Frame = 0


Query: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDG  +F+
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFN 174

[Close]

BLAST of CmoCh11G010400 vs. NCBI nr
Match: XP_023003075.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 334.7 bits (857), Expect = 6.3e-88
Identity = 167/174 (95.98%), Postives = 170/174 (97.70%), Query Frame = 0


Query: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFP+VNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEK SPQGNS
Sbjct: 1   MPSQFPIVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKVSPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDG  +F+
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFN 174

[Close]

BLAST of CmoCh11G010400 vs. NCBI nr
Match: XP_023530007.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.2 bits (853), Expect = 1.8e-87
Identity = 166/174 (95.40%), Postives = 171/174 (98.28%), Query Frame = 0


Query: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEI+ESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDG  +F+
Sbjct: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFN 174

[Close]

BLAST of CmoCh11G010400 vs. NCBI nr
Match: KAG6588366.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 331.3 bits (848), Expect = 7.0e-87
Identity = 167/174 (95.98%), Postives = 170/174 (97.70%), Query Frame = 0


Query: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCS+EEIEESWLGGEEIGAIF ESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDG  +F+
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFN 174

[Close]

BLAST of CmoCh11G010400 vs. NCBI nr
Match: KAG7022215.1 (putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 329.3 bits (843), Expect = 2.6e-86
Identity = 166/174 (95.40%), Postives = 170/174 (97.70%), Query Frame = 0


Query: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHK+SSISKEEVKFVMEKLEFFCS+EEIEESWLGGEEIGAIF ESEPSLEELKQ
Sbjct: 61  LESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDG  +F+
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFN 174

[Close]

BLAST of CmoCh11G010400 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 92.4 bits (228), Expect = 5.1e-19
Identity = 47/102 (46.08%), Postives = 69/102 (67.65%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGC 132
           IS+EE + VM  L  F +D++++E +   +E+ ++F E E SLEE+KQ F+VFD+N+DG 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 133 IDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFH 175
           IDA ELQ VL +L   QG ++ +C  MI   D NKDG  +F+
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFN 183

[Close]

BLAST of CmoCh11G010400 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.4e-16
Identity = 49/119 (41.18%), Postives = 71/119 (59.66%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSDEEIE--ESWLGGEEIGAIFYESEPSLEELKQTFNVFDK 129
           +  + +E+V  VM+ L    +D+E E  +     +E+  +F E EPSLEE+KQ F+VFD+
Sbjct: 88  DDGLCREDVGMVMKSLG-LSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDE 147

Query: 130 NRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEFHCRLEWQYGLLEF 187
           NRDG ID  +LQ VL +L   QG  + +C RMI  FD +KDG  +F       YG ++F
Sbjct: 148 NRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDF-------YGFVKF 198

[Close]

BLAST of CmoCh11G010400 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 67.8 bits (164), Expect = 1.3e-11
Identity = 42/117 (35.90%), Postives = 64/117 (54.70%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSDEE----IEESWLGG------EEIGAIF---YESEPSLE 129
           +  I+K+E+   +E L  +  D++    IE+  L G      EE G ++    E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 130 ELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEF 174
           ++++ FNVFD+NRDG I  +EL+SVL  L   QG  + DC RMI++ D + DG   F
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNF 134

[Close]

BLAST of CmoCh11G010400 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-09
Identity = 30/73 (41.10%), Postives = 42/73 (57.53%), Query Frame = 0


Query: 101 GEEIGAIFYESEPSLEELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMI 160
           GE    I  E +   E++K+ FNVFD+N DG I  DEL++VL  L   QG  + DC +MI
Sbjct: 63  GELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMI 122

Query: 161 ARFDHNKDGTTEF 174
            + D + DG   +
Sbjct: 123 KKVDVDGDGRVNY 135

[Close]

BLAST of CmoCh11G010400 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 57.4 bits (137), Expect = 1.8e-08
Identity = 39/116 (33.62%), Postives = 64/116 (55.17%), Query Frame = 0


Query: 63  SEATQHKNSSISKEEVKFVMEKLEF---FCSDEEIEESWLGGEEIGAIFYESEPSL--EE 122
           +E+ ++    I ++E+  +++K++     C D  IEE    GE    I  E E  +  E+
Sbjct: 27  NESFKNLGIIIPEDELTQIIQKIDVNGDGCVD--IEEF---GELYKTIMVEDEDEVGEED 86

Query: 123 LKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGTTEF 174
           +K+ FNVFD+N DG I  DEL++VL  L   QG  + +C +MI + D + DG   +
Sbjct: 87  MKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNY 137

The following BLAST results are available for this feature:

BLAST of CmoCh11G010400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	7.1e-18	46.08	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	1.9e-15	41.18	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9SRR7	1.9e-10	35.90	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9LNE7	2.3e-08	41.10	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9ZR02	2.5e-07	33.62	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of CmoCh11G010400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EIK8	1.2e-89	97.70	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1KQS0	3.0e-88	95.98	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]
A0A6J1J5Q6	1.2e-36	52.78	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1F854	2.3e-35	50.56	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1F5H5	9.6e-34	94.44	S-protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111441018 PE=3 SV=1	[more]

BLAST of CmoCh11G010400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927644.1	2.6e-89	97.70	probable calcium-binding protein CML30 [Cucurbita moschata]	[more]
XP_023003075.1	6.3e-88	95.98	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_023530007.1	1.8e-87	95.40	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
KAG6588366.1	7.0e-87	95.98	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. s...	[more]
KAG7022215.1	2.6e-86	95.40	putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. a...	[more]

BLAST of CmoCh11G010400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	5.1e-19	46.08	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	1.4e-16	41.18	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	1.3e-11	35.90	ARF-GAP domain 11	[more]
AT1G05990.1	1.6e-09	41.10	EF hand calcium-binding protein family	[more]
AT4G03290.1	1.8e-08	33.62	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 117..145 e-value: 2.4E-5 score: 33.7
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 117..143 e-value: 3.4E-7 score: 29.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 113..148 score: 14.987627
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 117..173 e-value: 1.14749E-9 score: 51.0093
None	No IPR available	GENE3D	1.10.238.10		coord: 45..189 e-value: 4.2E-13 score: 51.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..68
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..70
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 18..174
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 18..174
IPR010264	Plant self-incompatibility S1	PFAM	PF05938	Self-incomp_S1	coord: 160..226 e-value: 2.5E-8 score: 34.4
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 126..138
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 68..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh11G010400.1	CmoCh11G010400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060320	rejection of self pollen
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005509	calcium ion binding