CmoCh11G001800 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh11G001800
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cmo_Chr11: 872104 .. 872664 (+)
RNA-Seq Expression	CmoCh11G001800
Synteny	CmoCh11G001800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTCCACTTTCTCTCTCTTCTTTCTCTCTCCTCTCCTTGTAATTCTTGTCTCCTCCTTCTCCCATGGTTCGACCCCTCACAACATGGTTCAAGAAACATGCAAGAAATGTGCAGCCACCACTCCAAATCTCAGCTACGCCTTCTGTGTTTCGTCTTTAGAATCCGATGATCGGAGCCGCTCGGCAAGTCTCCACAGACTTGGGCTCATCTCCATGGATCTACTGCGCCACAACTTAACCAGCACACGACGTGAAGTCAAGAAGCTTTTGAGGAACAAGACATTGGATGGGTTCGTTAAGGCGTGTTTAGATGATTGTTTGGAGCTTTATTCTGATGCGATTCCGACGCTGAAAGAATGTAAAAGGGAGTATAAACAGAAGCGTTTTAGTGGTGCAAATGTGAAGGTTAGTTCAATCATGGAAGCTCCTTCAACTTGTGAAGATGGGTTTCATGAAAAACAAGGCGTGGTTTCACCGTTGACTAAGAATAATAAGAATGTTTTTCAGTTAGCTGCGTTGACTCTCTCCATCGTCAACATGAATATGCATCTTCAATGA

mRNA sequence

ATGATTTCCACTTTCTCTCTCTTCTTTCTCTCTCCTCTCCTTGTAATTCTTGTCTCCTCCTTCTCCCATGGTTCGACCCCTCACAACATGGTTCAAGAAACATGCAAGAAATGTGCAGCCACCACTCCAAATCTCAGCTACGCCTTCTGTGTTTCGTCTTTAGAATCCGATGATCGGAGCCGCTCGGCAAGTCTCCACAGACTTGGGCTCATCTCCATGGATCTACTGCGCCACAACTTAACCAGCACACGACGTGAAGTCAAGAAGCTTTTGAGGAACAAGACATTGGATGGGTTCGTTAAGGCGTGTTTAGATGATTGTTTGGAGCTTTATTCTGATGCGATTCCGACGCTGAAAGAATGTAAAAGGGAGTATAAACAGAAGCGTTTTAGTGGTGCAAATGTGAAGGTTAGTTCAATCATGGAAGCTCCTTCAACTTGTGAAGATGGGTTTCATGAAAAACAAGGCGTGGTTTCACCGTTGACTAAGAATAATAAGAATGTTTTTCAGTTAGCTGCGTTGACTCTCTCCATCGTCAACATGAATATGCATCTTCAATGA

Coding sequence (CDS)

ATGATTTCCACTTTCTCTCTCTTCTTTCTCTCTCCTCTCCTTGTAATTCTTGTCTCCTCCTTCTCCCATGGTTCGACCCCTCACAACATGGTTCAAGAAACATGCAAGAAATGTGCAGCCACCACTCCAAATCTCAGCTACGCCTTCTGTGTTTCGTCTTTAGAATCCGATGATCGGAGCCGCTCGGCAAGTCTCCACAGACTTGGGCTCATCTCCATGGATCTACTGCGCCACAACTTAACCAGCACACGACGTGAAGTCAAGAAGCTTTTGAGGAACAAGACATTGGATGGGTTCGTTAAGGCGTGTTTAGATGATTGTTTGGAGCTTTATTCTGATGCGATTCCGACGCTGAAAGAATGTAAAAGGGAGTATAAACAGAAGCGTTTTAGTGGTGCAAATGTGAAGGTTAGTTCAATCATGGAAGCTCCTTCAACTTGTGAAGATGGGTTTCATGAAAAACAAGGCGTGGTTTCACCGTTGACTAAGAATAATAAGAATGTTTTTCAGTTAGCTGCGTTGACTCTCTCCATCGTCAACATGAATATGCATCTTCAATGA

Protein sequence

MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ

Homology

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BLAST of CmoCh11G001800 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 7.9e-28
Identity = 64/153 (41.83%), Postives = 104/153 (67.97%), Query Frame = 0


Query: 29  NMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVK 88
           ++VQ TCKK A  +PN++Y FCV SL +D +S SA L  LG+IS +L     +  +  + 
Sbjct: 16  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKIQTFIG 75

Query: 89  KLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSGANVKVSSIMEAPSTCE 148
           ++L++K +D  +K  L+DC+ LY+DA  +++E   ++K K ++ ANVK+S+ ++   TCE
Sbjct: 76  RILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCE 135

Query: 149 DGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 182
           DGF EK+G+ SP+TK NK+  QL A++L+I  +
Sbjct: 136 DGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of CmoCh11G001800 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-27
Identity = 72/179 (40.22%), Postives = 118/179 (65.92%), Query Frame = 0


Query: 4   TFSL-FFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRS 63
           +FSL  F   LL++L +  S      ++VQ TCKK A  +PN++Y FCV SL +D +S +
Sbjct: 4   SFSLCIFFFNLLLLLQAVIS-----ADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHT 63

Query: 64  ASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECK 123
           A L  LG+IS +L   + +  +  + ++L++K +D  +K  L+DC+ LY+DA  +++E  
Sbjct: 64  ADLQGLGVISANLAIQHGSKIQTFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAI 123

Query: 124 REYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 182
            ++K K ++ ANVK+S+ ++   TCEDGF EK+G+VSP+TK NK+  QL A++L+I  +
Sbjct: 124 ADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of CmoCh11G001800 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.1e-16
Identity = 55/169 (32.54%), Postives = 91/169 (53.85%), Query Frame = 0


Query: 15  VILVSSFSHG-STPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLIS 74
           +++ S F +G +T   ++Q++CKK  A  P LSY FCV+SL  D +S++A +L  L L S
Sbjct: 7   LVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLAS 66

Query: 75  MDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSG 134
                  +T+ +  V + L+++     V+  L  CL  Y+DA   L       K + + G
Sbjct: 67  TKTAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRDYQG 126

Query: 135 ANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 182
           AN+ +S+ ++ P  CED F E +   SP+T  N  +F+   + L+  NM
Sbjct: 127 ANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of CmoCh11G001800 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-07
Identity = 50/160 (31.25%), Postives = 76/160 (47.50%), Query Frame = 0


Query: 6   SLFFLSPLLVILVSS----FSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSR 65
           SL FL  LLV L  S     S  S    +++ TCK     T N  Y FCVS+L+SD RS 
Sbjct: 4   SLIFL--LLVTLTFSASTLISAKSNTTTIIESTCK-----TTNY-YKFCVSALKSDPRSP 63

Query: 66  SASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKEC 125
           +A    L  I + +   N TST   +   L     D  +K  L DC E Y+ A  +L+  
Sbjct: 64  TADTKGLASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADSLRLT 123

Query: 126 KREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPL 162
            ++   + +  A++ V +  + P+ C + F   +G+  P+
Sbjct: 124 IQDLDDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPV 155

[Close]

BLAST of CmoCh11G001800 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-07
Identity = 39/135 (28.89%), Postives = 67/135 (49.63%), Query Frame = 0


Query: 48  AFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDC 107
           +FC S ++S+ ++  A L  L  I+    + + +   R+++ L++  T +  +K     C
Sbjct: 41  SFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTAT-NPTMKKAYTSC 100

Query: 108 LELYSDAIPTLKECKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKN 167
           ++ Y  AI +L + K+        G N+KVS+ ME PSTCE    + +  V P    N  
Sbjct: 101 VQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFK--VDPSAVKNSG 160

Query: 168 VFQ-LAALTLSIVNM 182
            FQ +  + L I NM
Sbjct: 161 DFQNICGIVLVISNM 172

[Close]

BLAST of CmoCh11G001800 vs. ExPASy TrEMBL
Match: A0A6J1EZS0 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.4e-97
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 60
           MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS
Sbjct: 1   MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 60

Query: 61  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 120
           RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE
Sbjct: 61  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 120

Query: 121 CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN 180
           CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN
Sbjct: 121 CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN 180

Query: 181 MNMHLQ 187
           MNMHLQ
Sbjct: 181 MNMHLQ 186

[Close]

BLAST of CmoCh11G001800 vs. ExPASy TrEMBL
Match: A0A6J1I7I4 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 8.9e-91
Identity = 177/186 (95.16%), Postives = 180/186 (96.77%), Query Frame = 0


Query: 1   MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 60
           MISTFSLFFLSPL VILVSS SHGSTPHNMVQETCKKCAATTPNLSY+FCVSSLESDDRS
Sbjct: 1   MISTFSLFFLSPLFVILVSSSSHGSTPHNMVQETCKKCAATTPNLSYSFCVSSLESDDRS 60

Query: 61  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 120
           RSA+LHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVK  LDDCLELYSDAIPTLKE
Sbjct: 61  RSANLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKVRLDDCLELYSDAIPTLKE 120

Query: 121 CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN 180
           CKREYKQKRFS ANVKVSSIMEAPSTCEDGFHEKQGV SPLTK+NKNVFQLAALTLSIVN
Sbjct: 121 CKREYKQKRFSDANVKVSSIMEAPSTCEDGFHEKQGVGSPLTKSNKNVFQLAALTLSIVN 180

Query: 181 MNMHLQ 187
           MNMHLQ
Sbjct: 181 MNMHLQ 186

[Close]

BLAST of CmoCh11G001800 vs. ExPASy TrEMBL
Match: A0A0A0LUJ3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 6.9e-67
Identity = 129/185 (69.73%), Postives = 159/185 (85.95%), Query Frame = 0


Query: 1   MISTFSLFFLSPLLVILVSSFSHG-STPHNMVQETCKKCAATTPNLSYAFCVSSLESDDR 60
           MIST  LF  + + +++ SSFS   S PHN++QETCKK AA+TPNL+Y FCV+SLESD R
Sbjct: 1   MISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTR 60

Query: 61  SRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLK 120
           SR A+LH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K CL DC+ELYSDA+PTLK
Sbjct: 61  SRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLK 120

Query: 121 ECKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIV 180
           E KREYK + +  AN+KVSSIMEAP+TCE+GF EK+G++SPLTKNN +VFQLAALTLSI+
Sbjct: 121 EAKREYKNRNYKDANIKVSSIMEAPTTCENGFKEKEGIISPLTKNNSDVFQLAALTLSII 180

Query: 181 NMNMH 185
           NMN+H
Sbjct: 181 NMNLH 185

[Close]

BLAST of CmoCh11G001800 vs. ExPASy TrEMBL
Match: A0A6J1C4E5 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 6.4e-65
Identity = 133/188 (70.74%), Postives = 158/188 (84.04%), Query Frame = 0


Query: 1   MISTFSLFFLSPLLVILVSSFSHGS--TPHNMVQETCKKCAATTPNLSYAFCVSSLESDD 60
           MISTFS   L+    +L  SFS GS  T  N++QETCK+ A +TPNLSY FCV+SLESDD
Sbjct: 27  MISTFS---LTTFFAVLFFSFSDGSTITTSNIIQETCKRSADSTPNLSYNFCVASLESDD 86

Query: 61  RSRSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTL 120
           RSR A LH+LGLI+MDL+RHN+TSTRR+++KLLRNK LD FVKA LDDCLELYSDA+P L
Sbjct: 87  RSRCADLHKLGLIAMDLMRHNITSTRRDIRKLLRNKKLDQFVKARLDDCLELYSDAVPIL 146

Query: 121 KECKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSI 180
           KE KR YK+K++  ANVKVSS+MEAPSTCEDGF +K+G+VSPLT+ NKNVF+LAALTLSI
Sbjct: 147 KEAKRCYKEKKYVDANVKVSSVMEAPSTCEDGFRDKEGLVSPLTEKNKNVFRLAALTLSI 206

Query: 181 VNMNMHLQ 187
           +NMN HLQ
Sbjct: 207 INMNTHLQ 211

[Close]

BLAST of CmoCh11G001800 vs. ExPASy TrEMBL
Match: A0A2K1XQD5 (PMEI domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G044100 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 7.9e-47
Identity = 94/178 (52.81%), Postives = 135/178 (75.84%), Query Frame = 0


Query: 4   TFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA 63
           TFS   L   L +L  +F H +   N++ ETCKKCAA  PNLSY FCVSSL++ +RS+  
Sbjct: 3   TFSYCLLLLFLPLLFCTF-HANIAQNLIPETCKKCAANDPNLSYNFCVSSLQASNRSQCD 62

Query: 64  SLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKR 123
           +L  LG++S+ L+++N+T+TR  VK LL+NK +D F++ACL+DCL+LYSDAIPTLK+   
Sbjct: 63  NLRGLGMMSIKLIKYNVTNTRHYVKNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMI 122

Query: 124 EYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 182
           +YK K +  AN++VSS+++A +TCEDGF +K+G VSPLTK N + FQL+A+ L+++NM
Sbjct: 123 DYKSKHYKDANIEVSSVIDAATTCEDGFEDKEGAVSPLTKRNNDTFQLSAIALALINM 179

[Close]

BLAST of CmoCh11G001800 vs. NCBI nr
Match: XP_022933437.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 60
           MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS
Sbjct: 1   MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 60

Query: 61  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 120
           RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE
Sbjct: 61  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 120

Query: 121 CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN 180
           CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN
Sbjct: 121 CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN 180

Query: 181 MNMHLQ 187
           MNMHLQ
Sbjct: 181 MNMHLQ 186

[Close]

BLAST of CmoCh11G001800 vs. NCBI nr
Match: XP_022973011.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 342.8 bits (878), Expect = 1.8e-90
Identity = 177/186 (95.16%), Postives = 180/186 (96.77%), Query Frame = 0


Query: 1   MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 60
           MISTFSLFFLSPL VILVSS SHGSTPHNMVQETCKKCAATTPNLSY+FCVSSLESDDRS
Sbjct: 1   MISTFSLFFLSPLFVILVSSSSHGSTPHNMVQETCKKCAATTPNLSYSFCVSSLESDDRS 60

Query: 61  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 120
           RSA+LHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVK  LDDCLELYSDAIPTLKE
Sbjct: 61  RSANLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKVRLDDCLELYSDAIPTLKE 120

Query: 121 CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN 180
           CKREYKQKRFS ANVKVSSIMEAPSTCEDGFHEKQGV SPLTK+NKNVFQLAALTLSIVN
Sbjct: 121 CKREYKQKRFSDANVKVSSIMEAPSTCEDGFHEKQGVGSPLTKSNKNVFQLAALTLSIVN 180

Query: 181 MNMHLQ 187
           MNMHLQ
Sbjct: 181 MNMHLQ 186

[Close]

BLAST of CmoCh11G001800 vs. NCBI nr
Match: KAG6587552.1 (hypothetical protein SDJN03_16117, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021528.1 hypothetical protein SDJN02_15253, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303.9 bits (777), Expect = 9.5e-79
Identity = 155/157 (98.73%), Postives = 155/157 (98.73%), Query Frame = 0


Query: 30  MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 89
           MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 60

Query: 90  LLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSGANVKVSSIMEAPSTCED 149
           LLRNKTLDGFVKA LDDCLELYSDAIPTLKECKREYKQKRFS ANVKVSSIMEAPSTCED
Sbjct: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFSDANVKVSSIMEAPSTCED 120

Query: 150 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 187
           GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ
Sbjct: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 157

[Close]

BLAST of CmoCh11G001800 vs. NCBI nr
Match: XP_023531537.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301.6 bits (771), Expect = 4.7e-78
Identity = 153/157 (97.45%), Postives = 155/157 (98.73%), Query Frame = 0


Query: 30  MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSASLHRLGLISMDLLRHNLTSTRREVKK 89
           MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA+LHRLGLISMDLLRHNLTSTRREVKK
Sbjct: 1   MVQETCKKCAATTPNLSYAFCVSSLESDDRSRSANLHRLGLISMDLLRHNLTSTRREVKK 60

Query: 90  LLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSGANVKVSSIMEAPSTCED 149
           LLRNKTLDGFVKA LDDCLELYSDAIPTLKECKREYKQKRF+ ANVKVSSIMEAPSTCED
Sbjct: 61  LLRNKTLDGFVKARLDDCLELYSDAIPTLKECKREYKQKRFNDANVKVSSIMEAPSTCED 120

Query: 150 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 187
           GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ
Sbjct: 121 GFHEKQGVVSPLTKNNKNVFQLAALTLSIVNMNMHLQ 157

[Close]

BLAST of CmoCh11G001800 vs. NCBI nr
Match: XP_038880152.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 277.7 bits (709), Expect = 7.3e-71
Identity = 135/186 (72.58%), Postives = 160/186 (86.02%), Query Frame = 0


Query: 1   MISTFSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 60
           MIST  LF  +  L+++ SS SHGS  HN++QETCKK A ++PN+SY FCV+S ESD+RS
Sbjct: 3   MISTSHLFSFTAFLLLIFSSSSHGSNTHNIIQETCKKSAESSPNISYKFCVASFESDNRS 62

Query: 61  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 120
           RSA+LH+LGLISMDL+RHN+TSTRRE+KKLLRNK L+ F+K CL DCLELYSDA+PTLKE
Sbjct: 63  RSANLHKLGLISMDLMRHNITSTRREIKKLLRNKKLEEFIKGCLKDCLELYSDAVPTLKE 122

Query: 121 CKREYKQKRFSGANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVN 180
            KREYK+K++  ANVKVSSIMEAP+TCEDGF EKQG +SPLT NN N FQLAALTLSI+N
Sbjct: 123 AKREYKEKKYKDANVKVSSIMEAPTTCEDGFQEKQGTISPLTNNNSNAFQLAALTLSIIN 182

Query: 181 MNMHLQ 187
           MNMHLQ
Sbjct: 183 MNMHLQ 188

[Close]

BLAST of CmoCh11G001800 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.3e-20
Identity = 64/167 (38.32%), Postives = 97/167 (58.08%), Query Frame = 0


Query: 5   FSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA- 64
           F++FFL      L+++F   +   +++Q+ CKK A   P + Y FCV SLE + +S++A 
Sbjct: 7   FAMFFL------LLNAF---TIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTAR 66

Query: 65  SLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKR 124
           SL RL ++S        TS +  V K+L+    + + +  L DCLELYSDA  +LKE   
Sbjct: 67  SLDRLVMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEALT 126

Query: 125 EYKQKRFSGANVKVSSIMEAPSTCEDGFHE-KQGVVSPLTKNNKNVF 170
             K + +  ANV +S+ M AP +CE GF E K+ + SP TK+N  +F
Sbjct: 127 IIKSRDYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of CmoCh11G001800 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-20
Identity = 55/167 (32.93%), Postives = 95/167 (56.89%), Query Frame = 0


Query: 16  ILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLISMD 75
           +L +  ++G T   +++ +CKK  AT+P   Y  CV+SLE++ ++++A  L  L + S  
Sbjct: 12  VLSNGLANGQT---LIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLVMASTK 71

Query: 76  LLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSGAN 135
                 T+ +  V K+++ K ++      L DCL+LY+DAI +L E     K + +    
Sbjct: 72  NAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRNYPTVK 131

Query: 136 VKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 182
             +S+ M+ PSTCE GF E++   SP+TK N N++Q+  + L+  NM
Sbjct: 132 TVLSAAMDTPSTCETGFKERK-APSPVTKENDNLYQMILIPLAFTNM 174

[Close]

BLAST of CmoCh11G001800 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.0e-19
Identity = 63/181 (34.81%), Postives = 90/181 (49.72%), Query Frame = 0


Query: 14  LVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS--RSASLHRLGLI 73
           ++ L    S   +  +M+ +TCK CAA +    Y FCVSSL +   +     +L  L L+
Sbjct: 9   IIFLFFLVSQVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSSLALV 68

Query: 74  SMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREY-KQKRF 133
            M     N T+T   +++LL +     F  ACL DCLELY DA   L+E  R +  +K  
Sbjct: 69  PMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEAVRVFITRKEL 128

Query: 134 SGANVKVSSIMEAPSTCEDGFHEKQ-------GV---VSPLTKNNKNVFQLAALTLSIVN 182
              NV VS+ ME+  TCE+GF E+        GV    SP+   N  +F+   + L I N
Sbjct: 129 GTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQIALCIFN 188

[Close]

BLAST of CmoCh11G001800 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.9e-17
Identity = 55/169 (32.54%), Postives = 91/169 (53.85%), Query Frame = 0


Query: 15  VILVSSFSHG-STPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA-SLHRLGLIS 74
           +++ S F +G +T   ++Q++CKK  A  P LSY FCV+SL  D +S++A +L  L L S
Sbjct: 7   LVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLAS 66

Query: 75  MDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKREYKQKRFSG 134
                  +T+ +  V + L+++     V+  L  CL  Y+DA   L       K + + G
Sbjct: 67  TKTAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRDYQG 126

Query: 135 ANVKVSSIMEAPSTCEDGFHEKQGVVSPLTKNNKNVFQLAALTLSIVNM 182
           AN+ +S+ ++ P  CED F E +   SP+T  N  +F+   + L+  NM
Sbjct: 127 ANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of CmoCh11G001800 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 85.1 bits (209), Expect = 6.4e-17
Identity = 58/179 (32.40%), Postives = 99/179 (55.31%), Query Frame = 0


Query: 5   FSLFFLSPLLVILVSSFSHGSTPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRSRSA- 64
           FSL+ +  +  +L++ F   +T  ++++++CKK     P L Y FCV SLE + +S++A 
Sbjct: 3   FSLYLV--MFFLLLNCF---ATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAK 62

Query: 65  SLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKECKR 124
           SL  L  +S        TS +  V K+L+    +  V+  L DCLELY++AI +L +   
Sbjct: 63  SLEGLVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAIDSLNQSLD 122

Query: 125 EYKQKRFSGANVKVSSIMEAPSTCEDGF-HEKQGVVSPLTKNNKNVFQLAALTLSIVNM 182
             K + +  A + +S+ M+AP +CE  F   K+ V SP TK N  +F +  + ++  +M
Sbjct: 123 TVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

The following BLAST results are available for this feature:

BLAST of CmoCh11G001800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9YUH4	7.9e-28	41.83	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q8GT41	1.8e-27	40.22	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
Q9FJR5	4.1e-16	32.54	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
O49603	1.2e-07	31.25	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702...	[more]
Q9LUV1	1.2e-07	28.89	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of CmoCh11G001800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EZS0	6.4e-97	100.00	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 ...	[more]
A0A6J1I7I4	8.9e-91	95.16	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV...	[more]
A0A0A0LUJ3	6.9e-67	69.73	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 S...	[more]
A0A6J1C4E5	6.4e-65	70.74	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4...	[more]
A0A2K1XQD5	7.9e-47	52.81	PMEI domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G04410...	[more]

BLAST of CmoCh11G001800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022933437.1	1.3e-96	100.00	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_022973011.1	1.8e-90	95.16	putative invertase inhibitor [Cucurbita maxima]	[more]
KAG6587552.1	9.5e-79	98.73	hypothetical protein SDJN03_16117, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023531537.1	4.7e-78	97.45	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
XP_038880152.1	7.3e-71	72.58	putative invertase inhibitor [Benincasa hispida]	[more]

BLAST of CmoCh11G001800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46970.1	1.3e-20	38.32	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	1.6e-20	32.93	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	4.0e-19	34.81	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	2.9e-17	32.54	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	6.4e-17	32.40	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 73..93
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 1..181
None	No IPR available	PANTHER	PTHR31707:SF314	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR	coord: 1..181
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..178 e-value: 3.4E-29 score: 113.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..181 e-value: 1.1E-40 score: 136.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..178 e-value: 2.8E-29 score: 102.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..182 e-value: 1.3E-38 score: 134.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..181
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 35..181 e-value: 1.5785E-51 score: 160.608

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh11G001800.1	CmoCh11G001800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity