CmoCh10G011230 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh10G011230 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.0e-50
Identity = 109/241 (45.23%), Postives = 149/241 (61.83%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M ++A S P+N   DI PR+IP     I  YGKNSF+W+GP P VH+M+PD +K  LS  
Sbjct: 62  MLKEAKSKPINLYDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKF 121

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           Y  QKP  NPL +LL  G+AD EG +W+    +    +    + +M+PA+  S +E++ K
Sbjct: 122 YQFQKPRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTK 181

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEE+VS  G +ELDV+P+L  L +DVISRTAFGSSYEEG+ IF   ++   L++++  T 
Sbjct: 182 WEEMVSTKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTI 241

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGE-AMHKDLLSILLQSNLKE 218
                             I+KE+K  L  +INKR +AM AGE + H DLL ILL+SN KE
Sbjct: 242 YLPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKE 301

BLAST of CmoCh10G011230 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.8e-47
Identity = 101/239 (42.26%), Postives = 143/239 (59.83%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M  +A   P+N S D   RV P ++ T+ KYGK+SF+WIGP PRV +MDP+ +K  +   
Sbjct: 66  MTRKAKLKPINLSDDPVLRVRPFIHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMVKS 125

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           Y   KP  NPL +L  DGLA+HEG  W+    +    +    +  M+PAM  SC EM+ K
Sbjct: 126 YKFNKPKRNPLVKLFADGLANHEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVSK 185

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           W++++SKDG  ELDV P L  L +DVIS TAFGSSYEEG ++F    +   LV+K++ + 
Sbjct: 186 WDKMISKDGSRELDVWPFLQRLTSDVISHTAFGSSYEEGNIVFELQTEQAELVMKTLQSV 245

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKE 217
                             I ++ ++ L+++INK+ +AM AGE    D+L ILL+SNLKE
Sbjct: 246 YIPGWSYLPTKRNRKMKEIDRKTQSCLMNIINKKTKAMQAGEGSTDDILGILLESNLKE 304

BLAST of CmoCh10G011230 vs. ExPASy Swiss-Prot
Match: Q05047 (Secologanin synthase OS=Catharanthus roseus OX=4058 GN=CYP72A1 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.4e-44
Identity = 95/242 (39.26%), Postives = 141/242 (58.26%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M ++A+S PM F++DI PR++P +NHTI+ YG+NSF W+G  PR+H+M+P+ +K  L+  
Sbjct: 65  MHQEALSKPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHS 124

Query: 61  YDIQK--PTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMI 120
              QK     NPL + L  G+   EG KWS    I    +    +  M+PA      +M+
Sbjct: 125 SKYQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDML 184

Query: 121 RKWEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVV---- 180
            KWE++  K G +E+D+ P    L +DVIS+ AFGS+YEEG  IF  LK+L +L +    
Sbjct: 185 TKWEKIAEKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMR 244

Query: 181 ---------------KSISTISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLK 218
                          K +  I+KE+  +L  +INKR +A+ AGE    DLL +LL+SN++
Sbjct: 245 DVYIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEPGEDDLLGVLLESNIQ 304

BLAST of CmoCh10G011230 vs. ExPASy Swiss-Prot
Match: U5NDT8 (Secologanin synthase OS=Catharanthus roseus OX=4058 GN=SLS PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.1e-44
Identity = 95/242 (39.26%), Postives = 141/242 (58.26%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M ++A+S+PM F +DI PR++P +NHTI  YG+NSF W+G  PR+H+M+P+ +K  L+  
Sbjct: 65  MHQEALSNPMEFDNDIVPRLMPHINHTIKTYGRNSFTWMGRIPRIHVMEPELIKEVLTHS 124

Query: 61  YDIQK--PTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMI 120
              QK     NPL + L  G+   EG KWS    I    +    +  M+PA      +M+
Sbjct: 125 SKYQKNFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDML 184

Query: 121 RKWEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVV---- 180
            KWE+L  K+G +E+D+ P    L +DVIS+ AFGS+Y+EG  IF  LK+L +L +    
Sbjct: 185 TKWEKLAEKEGSHEVDIFPTFDVLTSDVISKVAFGSTYDEGGKIFRLLKELMDLTIDCMR 244

Query: 181 ---------------KSISTISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLK 218
                          K +  I+KE+  +L  +INKR +A+ AGE    DLL +LL+SN++
Sbjct: 245 DVYIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEPGEDDLLGVLLESNIQ 304

BLAST of CmoCh10G011230 vs. ExPASy Swiss-Prot
Match: Q2MJ21 (Cytochrome P450 716A67 OS=Medicago truncatula OX=3880 GN=CYP72A67 PE=2 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 5.0e-42
Identity = 98/240 (40.83%), Postives = 135/240 (56.25%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M ++A S PM  S DI PRVIP +   +  YGKN F+W G  PR+ L +P+ +K  L+  
Sbjct: 64  MGKKAKSKPMELSDDIIPRVIPYIQQLVQIYGKNPFIWSGTTPRLILTEPELIKDVLNRT 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
            ++QKP    + + LF GL  HEG KW     +    +    +  M P+   SC +MI K
Sbjct: 124 SELQKPKYE-IFKFLFSGLIIHEGEKWRKHRRLMNAAFQLEKLKIMAPSFLTSCIDMISK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSI--- 180
           WE  +S DG  E+D+ P L NL +DVISR AFGSSYEEGK IF   ++   LV+K++   
Sbjct: 184 WESTLSSDGSGEIDIWPSLQNLTSDVISRNAFGSSYEEGKRIFDLQREQGELVMKNLVKS 243

Query: 181 ----------------STISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             I K++ + L  +INKR++AM AGEA   DLL +LL+SN +EI
Sbjct: 244 LIPLWRFIPTATQRRMHEIEKDIDSSLRYIINKREKAMKAGEATENDLLGLLLESNHQEI 302

BLAST of CmoCh10G011230 vs. ExPASy TrEMBL
Match: A0A6J1IHE1 (cytochrome P450 CYP72A219-like OS=Cucurbita maxima OX=3661 GN=LOC111473561 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.0e-98
Identity = 189/240 (78.75%), Postives = 200/240 (83.33%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAISSPMNFSHDIAPRVIPS+NHT HKYGKNSFMWIGPYPRVH+MDPDQLKAA SLM
Sbjct: 64  MREQAISSPMNFSHDIAPRVIPSINHTFHKYGKNSFMWIGPYPRVHIMDPDQLKAAFSLM 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           +D+QKP+INPL+RLLFDGL DHEGPKW     I    +    + DMVPAMCESCTEMIRK
Sbjct: 124 HDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEMIRK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEE+VSKD YYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQL NLVVK+ ST 
Sbjct: 184 WEEVVSKDEYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTV 243

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             ISKELKTLL+DVINKRQRAMMAGEAMH DLLSILLQSNLKEI
Sbjct: 244 YIPGWRFVPTKSNNIMKKISKELKTLLMDVINKRQRAMMAGEAMHNDLLSILLQSNLKEI 303

BLAST of CmoCh10G011230 vs. ExPASy TrEMBL
Match: A0A6J1EYN3 (cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111439752 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.0e-98
Identity = 189/240 (78.75%), Postives = 200/240 (83.33%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAISSPMNFSHDIAPRVIPS+NHTIHKYGKNSFMWIGPYPRVH+MDPDQLKAALSLM
Sbjct: 64  MREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPRVHIMDPDQLKAALSLM 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           +D+QKP+INPL+RLLFDGL DHEGPKW     I    +    +  MVPAMCESCTEMIRK
Sbjct: 124 HDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKHLKGMVPAMCESCTEMIRK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEE+VSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQL NLVVK+ ST 
Sbjct: 184 WEEVVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTV 243

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             ISKEL+ LLLDVINKRQRAMMAG AMH DLLSILLQSNLKEI
Sbjct: 244 YIPGWRFVPTKSNNRMKKISKELRDLLLDVINKRQRAMMAGAAMHNDLLSILLQSNLKEI 303

BLAST of CmoCh10G011230 vs. ExPASy TrEMBL
Match: A0A5D3CEG3 (Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003160 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 4.2e-68
Identity = 141/241 (58.51%), Postives = 170/241 (70.54%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M E+A S PMNFSHDI PRVIPSM+HTI KYGKNSFMW+GP PRV +MDP+QLKAALSL 
Sbjct: 61  MEEEAKSKPMNFSHDIVPRVIPSMHHTIKKYGKNSFMWLGPNPRVLIMDPEQLKAALSLY 120

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
            D QKPT NPL RLLFDGL +HEG KW     I  + +    + DMVP M ESC EMI K
Sbjct: 121 NDFQKPTTNPLVRLLFDGLINHEGEKWVKHRKIINHAFHFEKLKDMVPTMFESCNEMISK 180

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WE++ SKDG  ELDV+P+L +L ADVISRTAFGS+YEEGK+IF   K+L  LV+++ +T 
Sbjct: 181 WEKMTSKDGSCELDVVPYLQSLTADVISRTAFGSTYEEGKIIFNHFKELIRLVLQASNTV 240

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHK-DLLSILLQSNLKE 218
                             I+KE+KT+L+++I+KRQR M   E MH  DLL ILL+SN  E
Sbjct: 241 YLPGWRFLPTKSNNRMKKITKEIKTILMNIIDKRQRTMKEDETMHSDDLLGILLESNSNE 300

BLAST of CmoCh10G011230 vs. ExPASy TrEMBL
Match: A0A1S3BQL9 (cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103492242 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 4.2e-68
Identity = 141/241 (58.51%), Postives = 170/241 (70.54%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M E+A S PMNFSHDI PRVIPSM+HTI KYGKNSFMW+GP PRV +MDP+QLKAALSL 
Sbjct: 61  MEEEAKSKPMNFSHDIVPRVIPSMHHTIKKYGKNSFMWLGPNPRVLIMDPEQLKAALSLY 120

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
            D QKPT NPL RLLFDGL +HEG KW     I  + +    + DMVP M ESC EMI K
Sbjct: 121 NDFQKPTTNPLVRLLFDGLINHEGEKWVKHRKIINHAFHFEKLKDMVPTMFESCNEMISK 180

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WE++ SKDG  ELDV+P+L +L ADVISRTAFGS+YEEGK+IF   K+L  LV+++ +T 
Sbjct: 181 WEKMTSKDGSCELDVVPYLQSLTADVISRTAFGSTYEEGKIIFNHFKELIRLVLQASNTV 240

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHK-DLLSILLQSNLKE 218
                             I+KE+KT+L+++I+KRQR M   E MH  DLL ILL+SN  E
Sbjct: 241 YLPGWRFLPTKSNNRMKKITKEIKTILMNIIDKRQRTMKEDETMHSDDLLGILLESNSNE 300

BLAST of CmoCh10G011230 vs. ExPASy TrEMBL
Match: A0A6J1FEF7 (cytochrome P450 CYP72A219-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111443325 PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.9e-64
Identity = 129/240 (53.75%), Postives = 168/240 (70.00%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAIS PMNFS+DIAPRV PS++HTI KYGKNSFMW+GP PRVH+MDP+QLK   +L 
Sbjct: 62  MREQAISKPMNFSNDIAPRVTPSIHHTIQKYGKNSFMWLGPIPRVHIMDPEQLKTVFALF 121

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
            DIQKP++NPL +L   GL +HEG KW+    I    +    +  MVPA   SC +M+ K
Sbjct: 122 NDIQKPSMNPLVKLFAGGLVNHEGAKWAKHRKIINPAFHLEKLKGMVPAFYHSCYDMVSK 181

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSISTI 180
           WE +VS++G  +LDVMP+L  +AADVISRTAFGSSYE+GK IF    +L +LV+++I  I
Sbjct: 182 WESMVSEEGSCDLDVMPYLQQMAADVISRTAFGSSYEKGKKIFQLQTELADLVIQTIMRI 241

Query: 181 -------------------SKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                              SKE+ +L+L +IN+R+++M AGEAM+ DLL IL++SNL EI
Sbjct: 242 YIPGWRFLPTKSNKKMKELSKEISSLILGIINEREKSMKAGEAMNSDLLGILMESNLNEI 301

BLAST of CmoCh10G011230 vs. NCBI nr
Match: XP_023520477.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.2 bits (962), Expect = 3.9e-100
Identity = 193/240 (80.42%), Postives = 202/240 (84.17%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAISSPMNFSHDIAPRVIPSMNHTIH YGKNSFMWIGPYPRVH+MDPDQLKAA SLM
Sbjct: 64  MREQAISSPMNFSHDIAPRVIPSMNHTIHTYGKNSFMWIGPYPRVHIMDPDQLKAAFSLM 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           +DIQKP+INPL+RLLFDGLADHEGPKW     I    +    + DMVPAMCESCTEMIRK
Sbjct: 124 HDIQKPSINPLSRLLFDGLADHEGPKWVKHRKIINPAFHFKHLKDMVPAMCESCTEMIRK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQL NL+VK +ST 
Sbjct: 184 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLIVKVVSTV 243

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             ISKELKTLLL+VINKRQRAMMAGEAMH DLLSILLQSNLKEI
Sbjct: 244 YIPGWRFVPTKSNNRMKKISKELKTLLLNVINKRQRAMMAGEAMHNDLLSILLQSNLKEI 303

BLAST of CmoCh10G011230 vs. NCBI nr
Match: XP_022974803.1 (cytochrome P450 CYP72A219-like [Cucurbita maxima])

HSP 1 Score: 369.4 bits (947), Expect = 2.1e-98
Identity = 189/240 (78.75%), Postives = 200/240 (83.33%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAISSPMNFSHDIAPRVIPS+NHT HKYGKNSFMWIGPYPRVH+MDPDQLKAA SLM
Sbjct: 64  MREQAISSPMNFSHDIAPRVIPSINHTFHKYGKNSFMWIGPYPRVHIMDPDQLKAAFSLM 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           +D+QKP+INPL+RLLFDGL DHEGPKW     I    +    + DMVPAMCESCTEMIRK
Sbjct: 124 HDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEMIRK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEE+VSKD YYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQL NLVVK+ ST 
Sbjct: 184 WEEVVSKDEYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTV 243

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             ISKELKTLL+DVINKRQRAMMAGEAMH DLLSILLQSNLKEI
Sbjct: 244 YIPGWRFVPTKSNNIMKKISKELKTLLMDVINKRQRAMMAGEAMHNDLLSILLQSNLKEI 303

BLAST of CmoCh10G011230 vs. NCBI nr
Match: XP_022933047.1 (cytochrome P450 CYP72A219-like [Cucurbita moschata])

HSP 1 Score: 367.9 bits (943), Expect = 6.2e-98
Identity = 189/240 (78.75%), Postives = 200/240 (83.33%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAISSPMNFSHDIAPRVIPS+NHTIHKYGKNSFMWIGPYPRVH+MDPDQLKAALSLM
Sbjct: 64  MREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPRVHIMDPDQLKAALSLM 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           +D+QKP+INPL+RLLFDGL DHEGPKW     I    +    +  MVPAMCESCTEMIRK
Sbjct: 124 HDVQKPSINPLSRLLFDGLTDHEGPKWVKHRKIINPAFHFKHLKGMVPAMCESCTEMIRK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEE+VSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQL NLVVK+ ST 
Sbjct: 184 WEEVVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLVVKATSTV 243

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             ISKEL+ LLLDVINKRQRAMMAG AMH DLLSILLQSNLKEI
Sbjct: 244 YIPGWRFVPTKSNNRMKKISKELRDLLLDVINKRQRAMMAGAAMHNDLLSILLQSNLKEI 303

BLAST of CmoCh10G011230 vs. NCBI nr
Match: XP_023516285.1 (cytochrome P450 CYP72A219-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023516286.1 cytochrome P450 CYP72A219-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023516287.1 cytochrome P450 CYP72A219-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 365.9 bits (938), Expect = 2.4e-97
Identity = 189/240 (78.75%), Postives = 198/240 (82.50%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAISSPMNFSHDIAPRVIPS+NHTIHKYGKNSFMWIGPYP VH+MDPDQLKAALSLM
Sbjct: 64  MREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWIGPYPSVHIMDPDQLKAALSLM 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           +D++KP+INPL RLLFDGL DHEGPKW     I    +    + DMVPAMCESCTEMIRK
Sbjct: 124 HDVRKPSINPLQRLLFDGLTDHEGPKWVKHRKIINPAFHFKQLKDMVPAMCESCTEMIRK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEE VSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQL NL VK+ ST 
Sbjct: 184 WEEGVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLINLFVKATSTV 243

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             ISKELK LLLDVINKRQRAMMAGEAMH DLLSILLQSNLKEI
Sbjct: 244 YIPGWRFVPTKSNNRMKKISKELKALLLDVINKRQRAMMAGEAMHNDLLSILLQSNLKEI 303

BLAST of CmoCh10G011230 vs. NCBI nr
Match: XP_023515512.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.5 bits (924), Expect = 9.9e-96
Identity = 186/240 (77.50%), Postives = 200/240 (83.33%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MREQAISSPMNFSHDIAPRVIPS+NHTIHKYGKNSFMW+GPYPRVH+MDPDQLKAALSLM
Sbjct: 64  MREQAISSPMNFSHDIAPRVIPSINHTIHKYGKNSFMWVGPYPRVHIMDPDQLKAALSLM 123

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           +DIQKPTINPLARLLFDGL  HEGPKW     I    +    + DMVPA+CESCTEMIRK
Sbjct: 124 HDIQKPTINPLARLLFDGLTTHEGPKWVKHRKIMNPAFHFKHLKDMVPAVCESCTEMIRK 183

Query: 121 WEELVSKDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIST- 180
           WEE+VS++G+YELDVMPHL NLAADVISRTAFGSSYEEGKMIFIRLK+LTNLVV+SIST 
Sbjct: 184 WEEVVSEEGFYELDVMPHLENLAADVISRTAFGSSYEEGKMIFIRLKELTNLVVQSISTV 243

Query: 181 ------------------ISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKEI 218
                             ISKEL TLLLDVINKRQRAMMA EAM  DLLSILLQSNLK+I
Sbjct: 244 YISGWRFVPTKSNTRMKKISKELNTLLLDVINKRQRAMMASEAMPTDLLSILLQSNLKKI 303

BLAST of CmoCh10G011230 vs. TAIR 10
Match: AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )

HSP 1 Score: 176.8 bits (447), Expect = 1.9e-44
Identity = 100/237 (42.19%), Postives = 140/237 (59.07%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M EQ  S P+N + D   RV+P +  T+  +GK S+MW+GP   V +  P+ +K  L+ +
Sbjct: 66  MVEQEKSRPINLTDDYTHRVMPLIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVLNRV 125

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           YD  KP ++P+  L   G+A +EG KWS    I    +    +  M+PA  ESC+EMI K
Sbjct: 126 YDFPKPPVHPIVELFATGVALYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISK 185

Query: 121 WEELVSKDG-YYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSIS- 180
           WE+LV++ G   E+DV P+LG+L +DVISRTAFGSSYEEGK IF   ++    V+K++  
Sbjct: 186 WEKLVTEQGSSNEIDVWPYLGDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALEL 245

Query: 181 ------------------TISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSN 214
                              I+KE+K+ L ++I KRQR M  GEA   DLL ILL+SN
Sbjct: 246 AFIPGMRFLPTKNNLRMRQINKEVKSRLREIIMKRQRGMDTGEAPKNDLLGILLESN 302

BLAST of CmoCh10G011230 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-43
Identity = 94/240 (39.17%), Postives = 141/240 (58.75%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M  +A S P+  + DI+PRV+P        YG+  F W GP P + +MDP+Q+K   + +
Sbjct: 62  MLSEARSKPLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKV 121

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           YD QKP   PLA ++  GLA+++G KW+    I    +    I +MVPA  +SC E++ +
Sbjct: 122 YDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGE 181

Query: 121 WEELVS-KDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKS--- 180
           W++LVS K    E+DV P L ++ ADVISRTAFGSSY+EG+ IF    +L  L++++   
Sbjct: 182 WDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRK 241

Query: 181 ----------------ISTISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNLKE 217
                           +   ++E++ +L  ++NKR RA  AGEA   DLL ILL+SNL++
Sbjct: 242 AFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQ 301

BLAST of CmoCh10G011230 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 167.9 bits (424), Expect = 8.8e-42
Identity = 91/238 (38.24%), Postives = 138/238 (57.98%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           M  +A S P+N + DI PR++P     +  +G+  F W GP P + +MDP+Q+K   + +
Sbjct: 62  MLAEARSKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKV 121

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           YD QK    PL RL+  GL  ++G KW+    I    +    I +MVPA  +SC+E++ +
Sbjct: 122 YDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGE 181

Query: 121 WEELVS-KDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKS--- 180
           W++LV+ K    E+D+ P L ++ ADVISRTAFGSSY+EG+ IF    +L  L++++   
Sbjct: 182 WDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRK 241

Query: 181 ----------------ISTISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNL 215
                           +   ++E+K +L  ++NKR RA  AGEA   DLL ILL+SNL
Sbjct: 242 AIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNL 299

BLAST of CmoCh10G011230 vs. TAIR 10
Match: AT3G14650.1 (cytochrome P450, family 72, subfamily A, polypeptide 11 )

HSP 1 Score: 164.5 bits (415), Expect = 9.7e-41
Identity = 91/238 (38.24%), Postives = 138/238 (57.98%), Query Frame = 0

Query: 1   MREQAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLM 60
           MR +A S P+N + DI PR++P     +  +G+  F W G  P + +MDP+Q+   L+ +
Sbjct: 62  MRAEARSKPINLTDDITPRIVPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKV 121

Query: 61  YDIQKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRK 120
           YD QK    PL RL+  G+  ++G KW+    I    +    I +MVPA  +SC+E++ K
Sbjct: 122 YDFQKAHTFPLGRLIATGVLSYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCK 181

Query: 121 WEELVS-KDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKSI-- 180
           W++LVS K+   E+DV P L ++ ADVISRTAFGSS  EG+ IF    +L  L+++++  
Sbjct: 182 WDKLVSDKESSCEVDVWPGLVSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRK 241

Query: 181 -----------------STISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNL 215
                               ++E++ +L  ++NKR RA  AGEA + DLL ILL+SNL
Sbjct: 242 AFIPGYSYLPTKGNRRMKAKAREIQVILRGIVNKRLRAREAGEAPNDDLLGILLESNL 299

BLAST of CmoCh10G011230 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 161.0 bits (406), Expect = 1.1e-39
Identity = 90/235 (38.30%), Postives = 136/235 (57.87%), Query Frame = 0

Query: 4   QAISSPMNFSHDIAPRVIPSMNHTIHKYGKNSFMWIGPYPRVHLMDPDQLKAALSLMYDI 63
           +A S P+  + DI PRV+P     +  +G+ +  W GP P + +MDP+Q+K   + +YD 
Sbjct: 65  EATSKPIKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDF 124

Query: 64  QKPTINPLARLLFDGLADHEGPKWSNCSYIWLNCY----ISDMVPAMCESCTEMIRKWEE 123
           QK    PL+++L  GL  ++G KW+    I    +    I +MV    ESC+E++ +W++
Sbjct: 125 QKAHTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDK 184

Query: 124 LVS-KDGYYELDVMPHLGNLAADVISRTAFGSSYEEGKMIFIRLKQLTNLVVKS------ 183
           LVS K    E+DV P L ++ ADVISRTAFGSSY EG  IF    +L  LV+++      
Sbjct: 185 LVSDKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFI 244

Query: 184 -------------ISTISKELKTLLLDVINKRQRAMMAGEAMHKDLLSILLQSNL 215
                        + T ++E++ +L  +INKR+RA  +GEA  +DLL ILL+SNL
Sbjct: 245 PGYIYLPTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNL 299