CmoCh10G004470 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh10G004470
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDUF676 domain-containing protein
LocationCmo_Chr10: 2013927 .. 2016524 (+)
RNA-Seq ExpressionCmoCh10G004470
SyntenyCmoCh10G004470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCGCGGGCGCGGCCAATACTCCACTCCAGACCCCGCCATCGGGGCCTCTCTTCGACCCGTCAGCCACTCGCCATTGCCTTTCCAACCCTTCCCCTCGCTTCACCATTCGCTTTCCACCTAAGCCCTTCCCGGATCCTGCTCTCTCGCTTTCCAATCATTCCAGGATGGACTTGATCGGACGCCTGGCTAATAGTTTCTTCTGCAGCCTCCGCCGTCTGAAAGGCCTCAAGGTGGAGGCTGATTTCTCTGGCGAGGAAACTTTTGTCCCCGATGCCAAGGCGGCGCCCGAGCACCTTGTCATCATGGTCAATGGTCTAATTGGGAGGTATGCTCTCCTTCATTGCTCTTTGATTCTACTCTCATGCCTGCTCCCACCATATTACTAGCTGTCCAACTGTCGCTCGTTTGATTTAGCATGTTAATTTGCGATCGGATTTGGATTTGGGTATCCATCGATCCTTCTTTTTCTTCATTTCTTCAGTGCTGCAGATTGGAGATACGCGGCAGAGCAATTTGTAATGAAGCTTCCTGATAATGTTATCGTACACCGTAAGCGCTTACTATTCCCCCGATCTTCTATTCGTTTTGTAGGTCTCCTTGATAGGTTGCCACTTATTGTAAAAGTTCAAGGAGTCTCTATGCACTTGATGTGAAGATTTTTGTTATTTTGAGTGTGTTTACGAAAATATAGTGTCAAAAGCTTTTGACAAAAATGGGTTAGGTTTAGTCAATACAAGATTAATCGCATCCCGAAAGGATAATTTTGGGCTGAGAGATAATGATACTTTTCCAAATAATCTATCTAGCACAACTTTTATTCTAATTTTGGAATGATTTTGAAACTGATCCACCTTTAATATCCTTTTTCCAAAAGCAACCTTGAATGTGAAAAAAGTTATATCACGTGGCGGATGAAGCTAGGTTACCTCTAGGTGGTAAATCTGGATCCAATCTTTTGATTTCAATGTTGCTTAACCTATGACCACCATGCAGAGAAATAGCTAGTGATGGGGAAGCTGAATCAGTGTCAGTAGGGCGAATAGGATATCCATTAACCATCAGTGCAATTTTAGACCGCAAAATTGAACTCACCCCTTCACTCAAAAAATTGAGATTGTGTCCTAATGGGGATATCTCTTTGATTGTTTTGGTATACAGTGTGCTTAAAATTATGATTTAGTAAGCCTTTTTATTTGTGTTTCTACTCAAAAGTGTTTTTAGCTGATCCAAATTCCAATGCACATCTCTTTTCTTGACTCTCTGACATCAAGAGTAGTCTTTGAGAAATTCATAATTGAATTTACCTTTGTTGTGTTCAATTTATTGGCTAGTGTATCGTGTTGGAATTCCTTTATGTCAACCCTTTATGCGTACAGGTCACATTAATTTCTTTGCTTCTAAAATAATCTTCTCAAGCAGGCAGTGAGTGTAACACTTCAAGATTAACATTTGATGGCGTTGACACCATGGGAGAAAGGCTGGCTGAAGAGGTATCTTGGTCCTATATATGTATTTATGATTCATCTCTGAAAAGTATAAAAATAGCATCCTCACAGACATTTGGGTTCTCTCTTGAAATTGGTATTGTTGAATTTCTCAAAAGTTGTCGAGGACTTTACTTTAAACACATCCAATGCAGGTGTTAGCTGTCATAAGGCGTTGGCCAGAGGTGCAGAAAATTTCCTTTGTGGCCCATTCTTTAGGTGGGCTTGTTGCAAGGTATGCCATTGGAAGGCTTTTTGATCATATCCCTAAATTGGAATCCTCAGGTGCTCCTCAAAGTTTCTCTGCCGAAGAGCAGAAGCAACAGGTTGAGCAATTCCATCATGCAAGAATTGCTGGATTGGAGCCTGTGAACTTCATAACAGTTGCAACTCCACATTTAGGTTCACGTGGAAATAAACAGGTGATTATTTTTATTATATGCACTTTAGACTACAGAAACCAGATCGTCAAGCTGAAACTTGACAGAGTTGTTCATGAGTGTGCATTTTCCTTTGCATAGCAATAGGGGCTATTGCCTGTTGACCGTTAACTCGCCACCTCACCCATTATTCACGAGGTTGTTAAATAAACTTAAGGTCTACATCAAACCAGTCATCAGCCTGAAAATTCAGAGTTTTTCATTGAGTGTGCATTCACTTTGCCGCAGCATTGGGGTTGGGTGGAGGGAGGGTAGGATGAGGTTGGATTTATCAAATGAAATATCTAGTTAATATGACTTCTTTGTTCCTTTTGTTTCCTCAGTTTCCAGTTCTTTGTGGTCTTCCTTTCTTGGAGAGAAGAGCTTCTCAAACAGCACACTTAATTGCTGGGAGGTCTGGGAAGCACCTATTCCTCACTGATGATGAAAATGATGGGAAACCTCCGCTTCTCCTAAGAATGGTTACCGACTCCGAAGATCTAAAGTTCATGTATGTTTTGACTTTTGCATAAGAATAGTAAGTTGCCTATACTTTGTATGCTATTGGTGTTTGACTTTGTATATTGTCTTTGGACTTTTGTTGTTTAGCTCAGCATTACGTGCATTTGAACGTCGGGTGGCATACGCAAATGTGAATTATGATCATATCCTTTTACACTTAAGGGCTTGA

mRNA sequence

ATGATCGCGGGCGCGGCCAATACTCCACTCCAGACCCCGCCATCGGGGCCTCTCTTCGACCCGTCAGCCACTCGCCATTGCCTTTCCAACCCTTCCCCTCGCTTCACCATTCGCTTTCCACCTAAGCCCTTCCCGGATCCTGCTCTCTCGCTTTCCAATCATTCCAGGATGGACTTGATCGGACGCCTGGCTAATAGTTTCTTCTGCAGCCTCCGCCGTCTGAAAGGCCTCAAGGTGGAGGCTGATTTCTCTGGCGAGGAAACTTTTGTCCCCGATGCCAAGGCGGCGCCCGAGCACCTTGTCATCATGGTCAATGGTCTAATTGGGAGTGCTGCAGATTGGAGATACGCGGCAGAGCAATTTGTAATGAAGCTTCCTGATAATGTTATCGTACACCGCAGTGAGTGTAACACTTCAAGATTAACATTTGATGGCGTTGACACCATGGGAGAAAGGCTGGCTGAAGAGGTGTTAGCTGTCATAAGGCGTTGGCCAGAGGTGCAGAAAATTTCCTTTGTGGCCCATTCTTTAGGTGGGCTTGTTGCAAGGTATGCCATTGGAAGGCTTTTTGATCATATCCCTAAATTGGAATCCTCAGGTGCTCCTCAAAGTTTCTCTGCCGAAGAGCAGAAGCAACAGGTTGAGCAATTCCATCATGCAAGAATTGCTGGATTGGAGCCTGTGAACTTCATAACAGTTGCAACTCCACATTTAGGTTCACGTGGAAATAAACAGTTTCCAGTTCTTTGTGGTCTTCCTTTCTTGGAGAGAAGAGCTTCTCAAACAGCACACTTAATTGCTGGGAGGTCTGGGAAGCACCTATTCCTCACTGATGATGAAAATGATGGGAAACCTCCGCTTCTCCTAAGAATGGTTACCGACTCCGAAGATCTAAAGTTCATCTCAGCATTACGTGCATTTGAACGTCGGGTGGCATACGCAAATGTGAATTATGATCATATCCTTTTACACTTAAGGGCTTGA

Coding sequence (CDS)

ATGATCGCGGGCGCGGCCAATACTCCACTCCAGACCCCGCCATCGGGGCCTCTCTTCGACCCGTCAGCCACTCGCCATTGCCTTTCCAACCCTTCCCCTCGCTTCACCATTCGCTTTCCACCTAAGCCCTTCCCGGATCCTGCTCTCTCGCTTTCCAATCATTCCAGGATGGACTTGATCGGACGCCTGGCTAATAGTTTCTTCTGCAGCCTCCGCCGTCTGAAAGGCCTCAAGGTGGAGGCTGATTTCTCTGGCGAGGAAACTTTTGTCCCCGATGCCAAGGCGGCGCCCGAGCACCTTGTCATCATGGTCAATGGTCTAATTGGGAGTGCTGCAGATTGGAGATACGCGGCAGAGCAATTTGTAATGAAGCTTCCTGATAATGTTATCGTACACCGCAGTGAGTGTAACACTTCAAGATTAACATTTGATGGCGTTGACACCATGGGAGAAAGGCTGGCTGAAGAGGTGTTAGCTGTCATAAGGCGTTGGCCAGAGGTGCAGAAAATTTCCTTTGTGGCCCATTCTTTAGGTGGGCTTGTTGCAAGGTATGCCATTGGAAGGCTTTTTGATCATATCCCTAAATTGGAATCCTCAGGTGCTCCTCAAAGTTTCTCTGCCGAAGAGCAGAAGCAACAGGTTGAGCAATTCCATCATGCAAGAATTGCTGGATTGGAGCCTGTGAACTTCATAACAGTTGCAACTCCACATTTAGGTTCACGTGGAAATAAACAGTTTCCAGTTCTTTGTGGTCTTCCTTTCTTGGAGAGAAGAGCTTCTCAAACAGCACACTTAATTGCTGGGAGGTCTGGGAAGCACCTATTCCTCACTGATGATGAAAATGATGGGAAACCTCCGCTTCTCCTAAGAATGGTTACCGACTCCGAAGATCTAAAGTTCATCTCAGCATTACGTGCATTTGAACGTCGGGTGGCATACGCAAATGTGAATTATGATCATATCCTTTTACACTTAAGGGCTTGA

Protein sequence

MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLIGRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQFVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAYANVNYDHILLHLRA
Homology
BLAST of CmoCh10G004470 vs. ExPASy Swiss-Prot
Match: Q08448 (Lipid droplet phospholipase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LPL1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.1e-13
Identity = 64/232 (27.59%), Postives = 107/232 (46.12%), Query Frame = 0

Query: 98  EHLVIMVNGLIGS---AADWRYAAEQFVMK--LPDNVIVHRSECNTSRLTFDGVDTMGER 157
           +HL ++++GL G+       R      + K  + D++I    + N    TFDG++ +G R
Sbjct: 5   KHLFVLIHGLWGNYTHMESMRTILSTTLKKEDVNDDMIYFLPKQNAMFKTFDGIEIIGYR 64

Query: 158 LAEEVLAVIRRWPE--VQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQ 217
              EV   IR + +  + K+S + +S GGLVAR+ IG++     +L              
Sbjct: 65  TLIEVCEFIRDYKDGKITKLSVMGYSQGGLVARFMIGKMLTEFKEL-------------- 124

Query: 218 KQQVEQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHL---IA 277
                         +EP  FIT+ATPHLG     +F    G+ +     S    L   I 
Sbjct: 125 -----------FEDIEPQLFITMATPHLG----VEFYNPTGIAYKSALYSALRTLGSTIL 184

Query: 278 GRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAYANVNYD 320
           G+SG+ +F+ +  N+     +L  ++  E   ++ AL  F+ R+A+ANV  D
Sbjct: 185 GKSGREMFIANSSNN-----ILVKLSQGE---YLEALSLFKWRIAFANVKND 199

BLAST of CmoCh10G004470 vs. ExPASy Swiss-Prot
Match: P53118 (Putative lipase ROG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ROG1 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.3e-13
Identity = 67/229 (29.26%), Postives = 98/229 (42.79%), Query Frame = 0

Query: 98  EHLVIMVNGLIGS-AADWRYAAEQFV---MKLPDNVIVHRSECNTSRLTFDGVDTMGERL 157
           +HLV++ +GL  + + D  Y  EQ        P   IV +        T  GV  +G RL
Sbjct: 188 KHLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVKYLGTRL 247

Query: 158 AEEVLAVIRRWPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQ 217
           AE ++  +     ++KISFV HSLGGL+  +AI  +++  P                   
Sbjct: 248 AEYIIQDLYD-ESIRKISFVGHSLGGLIQAFAIAYIYEVYPWF----------------- 307

Query: 218 VEQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKH 277
                      + P+NFIT+A+P LG   +    +   L F           + G++G+ 
Sbjct: 308 --------FKKVNPINFITLASPLLGIVTDNPAYIKVLLSF----------GVIGKTGQD 367

Query: 278 LFLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAYANVNYDHIL 323
           L L +D   GKP L L        L  I  LR F+RR  YAN   D I+
Sbjct: 368 LGLENDVEVGKPLLYL-----LSGLPLIEILRRFKRRTVYANAINDGIV 375

BLAST of CmoCh10G004470 vs. ExPASy Swiss-Prot
Match: O14162 (Putative lipase C4A8.10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4A8.10 PE=3 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 5.7e-13
Identity = 65/231 (28.14%), Postives = 102/231 (44.16%), Query Frame = 0

Query: 99  HLVIMVNGLIGS-AADWRYAAEQFV--MKLPDNVIVHRSECNTSRLTFDGVDTMGERLAE 158
           HLV++ +G+  +  AD  Y  E+ +   K    ++V R        T  GV  +G+RL E
Sbjct: 306 HLVVLTHGMHSNVGADMEYLKEKLIESSKSVKELVVVRGFTGNYCQTEKGVRWLGKRLGE 365

Query: 159 EVLAVI----RRWPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQK 218
            +L +       +P    IS VAHSLGGLV  YA+G +                      
Sbjct: 366 WLLDITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYVH--------------------- 425

Query: 219 QQVEQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLIAGRSG 278
               + H A    + PV F+T+ATP LG  G  + P   G           ++ I G++G
Sbjct: 426 ---AKTHGAFFQAIHPVFFVTLATPWLGVAG--EHPSYIG--------KALSYGIIGKTG 485

Query: 279 KHLFLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAYANVNYDHIL 323
           + L LT   +  +    L +++D     F  A+  FE+R+ +AN   D+I+
Sbjct: 486 QDLSLTPLNHSIESRPFLVLMSD-PSTPFFQAVSFFEKRILFANTTNDYIV 501

BLAST of CmoCh10G004470 vs. ExPASy Swiss-Prot
Match: Q12103 (Putative lipase YDL109C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDL109C PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-09
Identity = 64/228 (28.07%), Postives = 95/228 (41.67%), Query Frame = 0

Query: 99  HLVIMVNGLIGS-AADWRYAAEQFV---MKLPDNVIVHRSECNTSRLTFDGVDTMGERLA 158
           HLVI+ +G   + +AD  Y  E+     M  P+  +V +     +  T  G+  +G  LA
Sbjct: 194 HLVILTHGFQSNVSADMEYLMEEIYKAQMNNPNERLVIKGYMKNACETEKGIKFLGVGLA 253

Query: 159 EEVLAVIRRWPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQV 218
             ++  +     V KISF+ HSLGGL   +AI  +    P                    
Sbjct: 254 NYIIDELYD-DSVGKISFIGHSLGGLTQTFAICYIKTKYPYF------------------ 313

Query: 219 EQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHL 278
                     +EP+NFI++A+P LG        +    P   + +      I G +G+ L
Sbjct: 314 -------FKKVEPINFISLASPLLG--------IATSTPNYVKMSLSMG--IIGTTGQEL 373

Query: 279 FLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAYANVNYDHIL 323
            L D  N G  PLL  +  +S     IS L  F+RR  YAN   D I+
Sbjct: 374 GLKDG-NYGDKPLLYLLSEES----LISVLARFKRRTLYANAVNDGIV 380

BLAST of CmoCh10G004470 vs. ExPASy Swiss-Prot
Match: Q9P2D6 (Protein FAM135A OS=Homo sapiens OX=9606 GN=FAM135A PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 5.0e-09
Identity = 50/176 (28.41%), Postives = 77/176 (43.75%), Query Frame = 0

Query: 83   FSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQFVMKLPDNVIVHRSECNTSRLT 142
            FS EE    D      HL++ V+GL G++AD R       + LP   I           T
Sbjct: 1236 FSVEE---EDGSEDGVHLIVCVHGLDGNSADLRLVKTYIELGLPGGRIDFLMSERNQNDT 1295

Query: 143  FDGVDTMGERLAEEVLAVIRRWP-EVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGA 202
            F   D+M +RL +E++  I+ +   V KISF+ HSLG L+ R  + R     P+      
Sbjct: 1296 FADFDSMTDRLLDEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTR-----PRF----- 1355

Query: 203  PQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLER 258
                     K  + + H           F++++ PHLG+  N    V  GL F+++
Sbjct: 1356 ---------KYYLNKLH----------TFLSLSGPHLGTLYNSSALVNTGLWFMQK 1379

BLAST of CmoCh10G004470 vs. ExPASy TrEMBL
Match: A0A6J1E5F9 (putative lipase YOR059C isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430104 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.2e-183
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYDH++
Sbjct: 301 ISALRAFERRVAYANVNYDHMV 322

BLAST of CmoCh10G004470 vs. ExPASy TrEMBL
Match: A0A6J1E1Z7 (putative lipase YOR059C isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430104 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.2e-183
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYDH++
Sbjct: 301 ISALRAFERRVAYANVNYDHMV 322

BLAST of CmoCh10G004470 vs. ExPASy TrEMBL
Match: A0A6J1E307 (uncharacterized protein LOC111430104 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430104 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.2e-183
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYDH++
Sbjct: 301 ISALRAFERRVAYANVNYDHMV 322

BLAST of CmoCh10G004470 vs. ExPASy TrEMBL
Match: A0A6J1E7G7 (putative lipase YOR059C isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111430104 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.3e-182
Identity = 320/322 (99.38%), Postives = 320/322 (99.38%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYD  L
Sbjct: 301 ISALRAFERRVAYANVNYDRRL 322

BLAST of CmoCh10G004470 vs. ExPASy TrEMBL
Match: A0A6J1JJS1 (putative lipase YOR059C isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111485732 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.5e-178
Identity = 310/322 (96.27%), Postives = 317/322 (98.45%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTP QTPPSGPLFDPS TRHCLSNP PRFTIRFPP+PFPDPALSLS+HSRMDLI
Sbjct: 1   MIAGAANTPFQTPPSGPLFDPSPTRHCLSNPFPRFTIRFPPRPFPDPALSLSDHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETF PD KAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFGPDVKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FV+KLPDNVIVHRSECNTSRLTFDGVDTMGERL+EEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVLKLPDNVIVHRSECNTSRLTFDGVDTMGERLSEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAF+RRVAYANVNYDH++
Sbjct: 301 ISALRAFKRRVAYANVNYDHMV 322

BLAST of CmoCh10G004470 vs. NCBI nr
Match: KAG6589725.1 (hypothetical protein SDJN03_15148, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023402.1 hypothetical protein SDJN02_14427 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 657.5 bits (1695), Expect = 5.9e-185
Identity = 324/327 (99.08%), Postives = 325/327 (99.39%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNP PRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPFPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FV KLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVTKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHILLHLRA 328
           ISALRAF+RRVAYANVNYDHILLHLRA
Sbjct: 301 ISALRAFKRRVAYANVNYDHILLHLRA 327

BLAST of CmoCh10G004470 vs. NCBI nr
Match: XP_022922032.1 (putative lipase YOR059C isoform X2 [Cucurbita moschata])

HSP 1 Score: 652.1 bits (1681), Expect = 2.5e-183
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYDH++
Sbjct: 301 ISALRAFERRVAYANVNYDHMV 322

BLAST of CmoCh10G004470 vs. NCBI nr
Match: XP_022922033.1 (putative lipase YOR059C isoform X3 [Cucurbita moschata] >XP_022922034.1 putative lipase YOR059C isoform X3 [Cucurbita moschata])

HSP 1 Score: 652.1 bits (1681), Expect = 2.5e-183
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYDH++
Sbjct: 301 ISALRAFERRVAYANVNYDHMV 322

BLAST of CmoCh10G004470 vs. NCBI nr
Match: XP_022922031.1 (uncharacterized protein LOC111430104 isoform X1 [Cucurbita moschata])

HSP 1 Score: 652.1 bits (1681), Expect = 2.5e-183
Identity = 320/322 (99.38%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYDH++
Sbjct: 301 ISALRAFERRVAYANVNYDHMV 322

BLAST of CmoCh10G004470 vs. NCBI nr
Match: XP_022922035.1 (putative lipase YOR059C isoform X4 [Cucurbita moschata])

HSP 1 Score: 648.7 bits (1672), Expect = 2.8e-182
Identity = 320/322 (99.38%), Postives = 320/322 (99.38%), Query Frame = 0

Query: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60
           MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI
Sbjct: 1   MIAGAANTPLQTPPSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRMDLI 60

Query: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120
           GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ
Sbjct: 61  GRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAAEQ 120

Query: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180
           FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL
Sbjct: 121 FVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGL 180

Query: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240
           VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS
Sbjct: 181 VARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGS 240

Query: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300
           RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF
Sbjct: 241 RGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKF 300

Query: 301 ISALRAFERRVAYANVNYDHIL 323
           ISALRAFERRVAYANVNYD  L
Sbjct: 301 ISALRAFERRVAYANVNYDRRL 322

BLAST of CmoCh10G004470 vs. TAIR 10
Match: AT4G25770.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 177/328 (53.96%), Postives = 225/328 (68.60%), Query Frame = 0

Query: 1   MIAGAANTPLQTP---PSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRM 60
           MIA  +   +  P   PS P F P +          R  ++FP +P         N  +M
Sbjct: 1   MIASFSRFQISIPDLIPSSPRFLPPSQNQFRF----RLYLQFPAQP--------KNLCQM 60

Query: 61  DLIGRLANSFFCSLRRLKGLKVEADFSGEETF---VPDAKAAPEHLVIMVNGLIGSAADW 120
           + + RL    F + R  + L  E D  GE+ F   V ++   P+HLV+MVNG++GSAADW
Sbjct: 61  EWMNRLGIGCFAANRSKRELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADW 120

Query: 121 RYAAEQFVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVA 180
           +YAAEQFV K PD V+VHRSE N++ LTFDGVD MGERLA EVL V++    ++KISFVA
Sbjct: 121 KYAAEQFVKKFPDKVLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVA 180

Query: 181 HSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVA 240
           HSLGGLVARYAIG+L++   +++S  +P   S E+  +  E      IAGLEP+NFIT A
Sbjct: 181 HSLGGLVARYAIGKLYEQPGEVDSLDSP---SKEKSARGGE------IAGLEPMNFITFA 240

Query: 241 TPHLGSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTD 300
           TPHLGSRG++QFP+LCGLPFLER ASQTAHL AGR+GKHLFL D++ DG  PLL+RM TD
Sbjct: 241 TPHLGSRGHRQFPILCGLPFLERTASQTAHLAAGRTGKHLFLVDND-DGNAPLLIRMATD 300

Query: 301 SEDLKFISALRAFERRVAYANVNYDHIL 323
           S+DLKFISAL AF+RRVAYANVN+D ++
Sbjct: 301 SDDLKFISALNAFKRRVAYANVNFDSMV 306

BLAST of CmoCh10G004470 vs. TAIR 10
Match: AT4G25770.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 177/328 (53.96%), Postives = 225/328 (68.60%), Query Frame = 0

Query: 1   MIAGAANTPLQTP---PSGPLFDPSATRHCLSNPSPRFTIRFPPKPFPDPALSLSNHSRM 60
           MIA  +   +  P   PS P F P +          R  ++FP +P         N  +M
Sbjct: 1   MIASFSRFQISIPDLIPSSPRFLPPSQNQFRF----RLYLQFPAQP--------KNLCQM 60

Query: 61  DLIGRLANSFFCSLRRLKGLKVEADFSGEETF---VPDAKAAPEHLVIMVNGLIGSAADW 120
           + + RL    F + R  + L  E D  GE+ F   V ++   P+HLV+MVNG++GSAADW
Sbjct: 61  EWMNRLGIGCFAANRSKRELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAADW 120

Query: 121 RYAAEQFVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVA 180
           +YAAEQFV K PD V+VHRSE N++ LTFDGVD MGERLA EVL V++    ++KISFVA
Sbjct: 121 KYAAEQFVKKFPDKVLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISFVA 180

Query: 181 HSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVA 240
           HSLGGLVARYAIG+L++   +++S  +P   S E+  +  E      IAGLEP+NFIT A
Sbjct: 181 HSLGGLVARYAIGKLYEQPGEVDSLDSP---SKEKSARGGE------IAGLEPMNFITFA 240

Query: 241 TPHLGSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTD 300
           TPHLGSRG++QFP+LCGLPFLER ASQTAHL AGR+GKHLFL D++ DG  PLL+RM TD
Sbjct: 241 TPHLGSRGHRQFPILCGLPFLERTASQTAHLAAGRTGKHLFLVDND-DGNAPLLIRMATD 300

Query: 301 SEDLKFISALRAFERRVAYANVNYDHIL 323
           S+DLKFISAL AF+RRVAYANVN+D ++
Sbjct: 301 SDDLKFISALNAFKRRVAYANVNFDSMV 306

BLAST of CmoCh10G004470 vs. TAIR 10
Match: AT1G10040.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 4.0e-70
Identity = 140/249 (56.22%), Postives = 178/249 (71.49%), Query Frame = 0

Query: 77  LKVEADFSGEETFV---PDAKAAPEHLVIMVNGLIGSAADWRYAAEQFVMKLPDNVIVHR 136
           L+ E+D SG        P  +  P HLV+MVNGLIGSA +WR+AA+Q + K P +++VH 
Sbjct: 56  LRAESDESGNVDLTVDFPGERTEPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLLVHC 115

Query: 137 SECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGLVARYAIGRLFDHI 196
           S+ N S  TFDGVD MGERLAEEV +VI+R P +QKISFV HSLGGL+ARYAIGRL++  
Sbjct: 116 SKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLYEQE 175

Query: 197 PKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLP 256
            + E    P +      K  +E+   ARIAGLEPV FIT ATPHLGSRG+KQ P+  G  
Sbjct: 176 SREE---LPHNSDDIGDKCSIEE-PKARIAGLEPVYFITSATPHLGSRGHKQVPLFSGSY 235

Query: 257 FLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAY 316
            LER A++ +  + G++GKHLFL D +  GKPPLLLRMV DS DLKFISAL+ F+RR+AY
Sbjct: 236 TLERLATRMSGCL-GKTGKHLFLADSDG-GKPPLLLRMVKDSRDLKFISALQCFKRRIAY 295

Query: 317 ANVNYDHIL 323
           AN ++DH++
Sbjct: 296 ANTSFDHLV 298

BLAST of CmoCh10G004470 vs. TAIR 10
Match: AT1G10040.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 4.0e-70
Identity = 140/249 (56.22%), Postives = 178/249 (71.49%), Query Frame = 0

Query: 77  LKVEADFSGEETFV---PDAKAAPEHLVIMVNGLIGSAADWRYAAEQFVMKLPDNVIVHR 136
           L+ E+D SG        P  +  P HLV+MVNGLIGSA +WR+AA+Q + K P +++VH 
Sbjct: 56  LRAESDESGNVDLTVDFPGERTEPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLLVHC 115

Query: 137 SECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLGGLVARYAIGRLFDHI 196
           S+ N S  TFDGVD MGERLAEEV +VI+R P +QKISFV HSLGGL+ARYAIGRL++  
Sbjct: 116 SKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLYEQE 175

Query: 197 PKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLP 256
            + E    P +      K  +E+   ARIAGLEPV FIT ATPHLGSRG+KQ P+  G  
Sbjct: 176 SREE---LPHNSDDIGDKCSIEE-PKARIAGLEPVYFITSATPHLGSRGHKQVPLFSGSY 235

Query: 257 FLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAY 316
            LER A++ +  + G++GKHLFL D +  GKPPLLLRMV DS DLKFISAL+ F+RR+AY
Sbjct: 236 TLERLATRMSGCL-GKTGKHLFLADSDG-GKPPLLLRMVKDSRDLKFISALQCFKRRIAY 295

Query: 317 ANVNYDHIL 323
           AN ++DH++
Sbjct: 296 ANTSFDHLV 298

BLAST of CmoCh10G004470 vs. TAIR 10
Match: AT5G51180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.0e-66
Identity = 128/228 (56.14%), Postives = 160/228 (70.18%), Query Frame = 0

Query: 95  AAPEHLVIMVNGLIGSAADWRYAAEQFVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLA 154
           ++ +HLV+MV+G++GS  DW++ AEQFV K+PD V VH SE N S LT DGVD MGERLA
Sbjct: 31  SSADHLVVMVHGILGSTDDWKFGAEQFVKKMPDKVFVHCSEKNVSALTLDGVDVMGERLA 90

Query: 155 EEVLAVIRRWPEVQKISFVAHSLGGLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQV 214
            EVL +I+R P + KISFVAHSLGGL ARYAIG+L+    + +   +    S E  K   
Sbjct: 91  AEVLDIIQRKPNICKISFVAHSLGGLAARYAIGKLYKPANQEDVKDSVADSSQETPK--- 150

Query: 215 EQFHHARIAGLEPVNFITVATPHLGSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHL 274
                  I GLE +NFITVATPHLGS GNKQ P L G   +E+ A    H I  R+G+HL
Sbjct: 151 -----GTICGLEAMNFITVATPHLGSMGNKQVPFLFGFSSIEKVAGLIIHWIFKRTGRHL 210

Query: 275 FLTDDENDGKPPLLLRMVTDSEDLKFISALRAFERRVAYANVNYDHIL 323
           FL D+E +GKPPLL RMV D++D  FISALRAF+RRVAY+NV +DH++
Sbjct: 211 FLKDEE-EGKPPLLRRMVEDTDDCHFISALRAFKRRVAYSNVGHDHVV 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q084481.1e-1327.59Lipid droplet phospholipase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / ... [more]
P531184.3e-1329.26Putative lipase ROG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX... [more]
O141625.7e-1328.14Putative lipase C4A8.10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O... [more]
Q121031.0e-0928.07Putative lipase YDL109C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c)... [more]
Q9P2D65.0e-0928.41Protein FAM135A OS=Homo sapiens OX=9606 GN=FAM135A PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1E5F91.2e-18399.38putative lipase YOR059C isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430104... [more]
A0A6J1E1Z71.2e-18399.38putative lipase YOR059C isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430104... [more]
A0A6J1E3071.2e-18399.38uncharacterized protein LOC111430104 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1E7G71.3e-18299.38putative lipase YOR059C isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111430104... [more]
A0A6J1JJS11.5e-17896.27putative lipase YOR059C isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111485732 P... [more]
Match NameE-valueIdentityDescription
KAG6589725.15.9e-18599.08hypothetical protein SDJN03_15148, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022922032.12.5e-18399.38putative lipase YOR059C isoform X2 [Cucurbita moschata][more]
XP_022922033.12.5e-18399.38putative lipase YOR059C isoform X3 [Cucurbita moschata] >XP_022922034.1 putative... [more]
XP_022922031.12.5e-18399.38uncharacterized protein LOC111430104 isoform X1 [Cucurbita moschata][more]
XP_022922035.12.8e-18299.38putative lipase YOR059C isoform X4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G25770.11.4e-8853.96alpha/beta-Hydrolases superfamily protein [more]
AT4G25770.21.4e-8853.96alpha/beta-Hydrolases superfamily protein [more]
AT1G10040.14.0e-7056.22alpha/beta-Hydrolases superfamily protein [more]
AT1G10040.24.0e-7056.22alpha/beta-Hydrolases superfamily protein [more]
AT5G51180.12.0e-6656.14alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007751Domain of unknown function DUF676, lipase-likePFAMPF05057DUF676coord: 97..320
e-value: 9.7E-65
score: 218.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 100..276
e-value: 1.7E-14
score: 56.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 99..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR12482:SF55ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN-RELATEDcoord: 52..321
IPR044294Lipase-likePANTHERPTHR12482UNCHARACTERIZEDcoord: 52..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh10G004470.1CmoCh10G004470.1mRNA