CmoCh10G003500 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh10G003500 vs. ExPASy Swiss-Prot
Match: B0F9L7 (Golgin candidate 2 OS=Arabidopsis thaliana OX=3702 GN=GC2 PE=1 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 3.8e-136
Identity = 339/707 (47.95%), Postives = 456/707 (64.50%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNL-EAIGKTGDI-LPLKDQLKKK 60
           MANWISSKLKAAE+ILQQ+DQQAA+SL+K E+    D + E   K+G   + LKDQL+KK
Sbjct: 1   MANWISSKLKAAETILQQLDQQAADSLRKDEKSETHDEVFETSPKSGSSPVSLKDQLRKK 60

Query: 61  NQVDNDY-HGKLRSDLSLNVS--------RNQDNVISAASKPSPS--SKPSTLTDSDWTE 120
               +D   G  R+      S        R  D      S PS S     + LTD+DWTE
Sbjct: 61  TYEGSDSGSGSQRNSTEQKPSYLSSSKKVRKPDQSHERTSAPSQSLTQDNTKLTDNDWTE 120

Query: 121 LLGIPNQ-PSTSTASRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGE 180
           LL  PNQ  STST+    G+  +RG K+D +R  N G +  V D KK+ ++   N     
Sbjct: 121 LLSTPNQRTSTSTSRSPGGTSAIRGLKKDGKRHGNLGKNPLVSDGKKSSSSNVVNSRGRP 180

Query: 181 RKKLNRKTNDVEESNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEK 240
           +K+ N++ +D E S       S S  D+KN              ++A  +I V +   E 
Sbjct: 181 QKQTNKEPSDKEVS-------SPSDADMKN--------------RNAPRDIFVNSTHKE- 240

Query: 241 NEIGGNFDSKGFSSEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGK 300
                    K  S +   L      ++ET+P      R +  +    + +  + S   GK
Sbjct: 241 -------SEKDVSGKTPPLDDSRRSANETLP------RETSPSVGKRDGRESRRSSVWGK 300

Query: 301 HKSDEVSRSSISDGV-RKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILA 360
              +EVS+S++SDG+ RKE   SS S   S SD +S S++D+E ER++EE +RRR+++ A
Sbjct: 301 QVREEVSQSNVSDGLTRKE---SSLSSDESESDYESDSSTDSERERQREERRRRRERVFA 360

Query: 361 EKAAAKAMEAIKEHEDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELE 420
           EK A KA+  IKE E++VARLEGEK SLEKI+E+RA++QA+EA+ELQT+MME +EA +LE
Sbjct: 361 EKVATKAVAVIKERENMVARLEGEKLSLEKIVEERAKQQAQEAAELQTNMMETLEAADLE 420

Query: 421 KQKHNETRREALAKMVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEEL 480
           KQKHN TR E L ++  LE ENA+L ++LA+ Q  LE + ++VA L+QQ+ELKE+  EEL
Sbjct: 421 KQKHNNTRMEVLTRLAGLEAENAELTRSLAAGQKKLETQIDQVAVLKQQVELKESTLEEL 480

Query: 481 RRRIASSQEAGTSTKPL-AFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRK 540
           +R   +    GT+ K L   +G +FE ++LEAE SL+TDK+ +LQ+K  KLE DIE++RK
Sbjct: 481 KRNTFNIGGRGTTLKQLDTSRGDKFEHQMLEAEISLLTDKIGRLQDKATKLEADIEMMRK 540

Query: 541 EMEEPTEVEVELKRRLGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNT 600
           E+EEPTEVE+ELKRRL Q+TDHLIQKQ QVEALSSEKAT+LFRIEAV+R +EE+K M   
Sbjct: 541 ELEEPTEVEIELKRRLNQLTDHLIQKQSQVEALSSEKATILFRIEAVSRLIEENKGM--- 600

Query: 601 NDITSTSWRDLESGKWKLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNP 660
              T  S +DLE+G W+LSGSK +P  +DKI SGKKHLG L+ QL+AI+ +G VF+RRNP
Sbjct: 601 -SATEASSQDLEAGDWELSGSKFKPAFQDKIRSGKKHLGWLVMQLNAIFISGTVFLRRNP 660

Query: 661 AAKLWSVVYLICLHLWVLYIFMSHSRVDT--ESKSGAVISLENINTS 690
            AK+W+VVYL+CLHLWVLYI +SHS   +  E +SGAVISLEN + S
Sbjct: 661 TAKIWAVVYLVCLHLWVLYILLSHSDASSSGELRSGAVISLENFSNS 665

BLAST of CmoCh10G003500 vs. ExPASy Swiss-Prot
Match: Q9QYE6 (Golgin subfamily A member 5 OS=Mus musculus OX=10090 GN=Golga5 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.2e-09
Identity = 83/365 (22.74%), Postives = 183/365 (50.14%), Query Frame = 0

Query: 319 SDSGSASDAEIEREKEEIKRRRQKILAEKAAAK-AMEAIKEHEDLVARLEGEKQSLEKIL 378
           +++ + ++ +   EK+++   +Q++   +A+ + A + + +++    R+    QS EK++
Sbjct: 388 AEAVTLAERKYSEEKKKVDELQQQVKLHRASLESAKQELVDYKQKATRI---LQSKEKLI 447

Query: 379 EDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKMVKLETENADLAKTLASV 438
                  + E  E  T+    +E +  EK+   E  ++ + +M +L +E  D        
Sbjct: 448 NSLKEGSSFEGLESSTASSMELEELRHEKEMQKEEIQKLMGQMHQLRSELQD-------- 507

Query: 439 QWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTKPLAFKGIEFELEILEAE 498
                +E  +V+      E  +   +++ ++  S QE            +E ELE ++ E
Sbjct: 508 -----MEAQQVSEAESAREQLQDLQDQIAKQRTSKQE------------LETELERMKQE 567

Query: 499 HSLITDKVLQ----LQEKGKKLEEDIELVRKEMEEPT---EVEVELKRRLGQMTDHLIQK 558
              + + + +    LQ + K  EE+I+ +R ++   T     + EL+ RL Q+T+ LIQK
Sbjct: 568 FRYMEEDLHRTKNTLQSRIKDREEEIQKLRNQLTNKTLSNSSQSELESRLHQLTETLIQK 627

Query: 559 QVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKWKLSGSKLR-- 618
           Q  +E+LS+EK +L+F++E + +Q+  + S  N+    + S   ++SG+    G++LR  
Sbjct: 628 QTMLESLSTEKNSLVFQLERLEQQVHSASSGPNSGSAINMS--GVDSGE----GTRLRNV 687

Query: 619 PMLEDKIHSGKKHLGSLIQQ----LDAIYAAGMVFIRRNPAAKLWSVVYLICLHLWVLYI 670
           P+L +   +    +   +++    +D       +F+RR P A+++ ++Y+  LHLWV+ +
Sbjct: 688 PVLFNDTETNLAGMYGKVRKAASSIDQFSIRLGIFLRRYPIARVFVIIYMALLHLWVMIV 718

BLAST of CmoCh10G003500 vs. ExPASy Swiss-Prot
Match: Q8TBA6 (Golgin subfamily A member 5 OS=Homo sapiens OX=9606 GN=GOLGA5 PE=1 SV=3)

HSP 1 Score: 58.2 bits (139), Expect = 4.4e-07
Identity = 90/386 (23.32%), Postives = 186/386 (48.19%), Query Frame = 0

Query: 318 DSDSGSASDAEIEREKEEIKRRRQKI-----LAEKAAAKAMEAIKEHEDLV----ARLEG 377
           + +S     +E      +++  RQ +     LAE+  +   + + E +  V      LE 
Sbjct: 364 EQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLES 423

Query: 378 EKQSL-------EKILEDRAR-----KQAEEASELQTSMMEMMEAVELEKQKHNETRREA 437
            KQ L        +IL+ + +     K+      L +S    ME  EL  +K  E +RE 
Sbjct: 424 SKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEK--EMQREE 483

Query: 438 LAKMVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAG 497
           + K++        + +  + +Q   ++E  +V       E  +  H+++  + AS QE  
Sbjct: 484 IQKLM------GQIHQLRSELQ---DMEAQQVNEAESAREQLQDLHDQIAGQKASKQE-- 543

Query: 498 TSTKPLAFKGIEFELEILEAEHSLITDKVLQ----LQEKGKKLEEDIELVRKEMEEPT-- 557
                     +E ELE L+ E   I + + +    LQ + K  +E+I+ +R ++   T  
Sbjct: 544 ----------LETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLS 603

Query: 558 -EVEVELKRRLGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITS 617
              + EL+ RL Q+T+ LIQKQ  +E+LS+EK +L+F++E + +Q+  +    ++++ +S
Sbjct: 604 NSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASG--SSSNGSS 663

Query: 618 TSWRDLESGKWKLSGSKLR--PMLEDKIHSGKKHLGSLIQQ----LDAIYAAGMVFIRRN 670
            +   +++G+    G++LR  P+L +   +    +   +++    +D       +F+RR 
Sbjct: 664 INMSGIDNGE----GTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFSIRLGIFLRRY 720

BLAST of CmoCh10G003500 vs. ExPASy TrEMBL
Match: A0A6J1E702 (golgin candidate 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430004 PE=4 SV=1)

HSP 1 Score: 1259.2 bits (3257), Expect = 0.0e+00
Identity = 692/692 (100.00%), Postives = 692/692 (100.00%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60

Query: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120
           VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA
Sbjct: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120

Query: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180
           SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES
Sbjct: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180

Query: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240
           NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS
Sbjct: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240

Query: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300
           EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG
Sbjct: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300

Query: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360
           VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE
Sbjct: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360

Query: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420
           DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM
Sbjct: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420

Query: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480
           VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK
Sbjct: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480

Query: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540
           PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL
Sbjct: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540

Query: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600
           GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW
Sbjct: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600

Query: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660
           KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW
Sbjct: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660

Query: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           VLYIFMSHSRVDTESKSGAVISLENINTSLNM
Sbjct: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 692

BLAST of CmoCh10G003500 vs. ExPASy TrEMBL
Match: A0A6J1JFU1 (golgin candidate 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485352 PE=4 SV=1)

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 678/693 (97.84%), Postives = 687/693 (99.13%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQL-KKKN 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQL KKKN
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKKN 60

Query: 61  QVDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTST 120
           QVDNDYHGKLRSDLSLNVSRNQDNVISAA KPSP+SKPSTLTDSDWTELLGIPNQPSTST
Sbjct: 61  QVDNDYHGKLRSDLSLNVSRNQDNVISAAPKPSPTSKPSTLTDSDWTELLGIPNQPSTST 120

Query: 121 ASRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEE 180
           ASRSNGSPTVRGAKRDVRRPSNAGS+MSVLDFKKTQNNT+SNRSVGERKKL RKT+DVEE
Sbjct: 121 ASRSNGSPTVRGAKRDVRRPSNAGSNMSVLDFKKTQNNTKSNRSVGERKKLTRKTSDVEE 180

Query: 181 SNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFS 240
           SNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFS
Sbjct: 181 SNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFS 240

Query: 241 SEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISD 300
           SEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISD
Sbjct: 241 SEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISD 300

Query: 301 GVRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEH 360
           GVRKEWTGSSTSGGSSG DSDS SASDAEIERE+EEIKRRRQKILAEKAAAKAMEAIKEH
Sbjct: 301 GVRKEWTGSSTSGGSSGLDSDSDSASDAEIEREREEIKRRRQKILAEKAAAKAMEAIKEH 360

Query: 361 EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK 420
           EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK
Sbjct: 361 EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK 420

Query: 421 MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST 480
           MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST
Sbjct: 421 MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST 480

Query: 481 KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRR 540
           KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEE IELVRKEMEEPTEVEVELKRR
Sbjct: 481 KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEESIELVRKEMEEPTEVEVELKRR 540

Query: 541 LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGK 600
           LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTN+I+STSWRDLESGK
Sbjct: 541 LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNNISSTSWRDLESGK 600

Query: 601 WKLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHL 660
           WKLSGSKL+PMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHL
Sbjct: 601 WKLSGSKLKPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHL 660

Query: 661 WVLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           WVLYIFMSHS+VDTESKSGAVISLENINTSLNM
Sbjct: 661 WVLYIFMSHSQVDTESKSGAVISLENINTSLNM 693

BLAST of CmoCh10G003500 vs. ExPASy TrEMBL
Match: A0A6J1BZG9 (golgin candidate 2 OS=Momordica charantia OX=3673 GN=LOC111007018 PE=4 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 595/692 (85.98%), Postives = 645/692 (93.21%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVD+LEA  KTGDILPLKDQLKKKNQ
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDSLEATSKTGDILPLKDQLKKKNQ 60

Query: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120
           +DNDYHGK+RSDLSLNVSRNQDNVISA SKPSP  K  TLTDSDWTELLG PNQPSTS A
Sbjct: 61  LDNDYHGKVRSDLSLNVSRNQDNVISAVSKPSP--KSPTLTDSDWTELLGTPNQPSTSAA 120

Query: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180
           SRSNGS ++RG KRD RRP+N GS++SVLDFKKTQ NT+SN+S+GE KKLNRK +DVEES
Sbjct: 121 SRSNGSSSIRGVKRDGRRPNNGGSNISVLDFKKTQ-NTKSNKSIGETKKLNRKASDVEES 180

Query: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240
           NISVSLG+NSRVD KNDKN+ HSEG+ELDKK+AGGN++V+AKSVEK EIGG+FDSKGF+ 
Sbjct: 181 NISVSLGTNSRVDPKNDKNVAHSEGQELDKKEAGGNMLVQAKSVEKKEIGGDFDSKGFTL 240

Query: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300
           E S L IRN QSSETVPD DQAKRISDMN+I+ +AQ+H+ SG +GKHKSDEVSRSSISD 
Sbjct: 241 EKSVLAIRNGQSSETVPDRDQAKRISDMNSIMKDAQNHRGSGVSGKHKSDEVSRSSISDD 300

Query: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360
           VRKEWTGSSTS GSSGSDSDSGSASD EIERE+EE +++R+K LAEKAAAKAMEAIKE E
Sbjct: 301 VRKEWTGSSTSDGSSGSDSDSGSASDFEIEREREERRKKREKFLAEKAAAKAMEAIKEQE 360

Query: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420
           DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMME MEAVELEKQKHNETRREALA M
Sbjct: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMETMEAVELEKQKHNETRREALAIM 420

Query: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480
            KLETENADLA+ LASVQWNLELEG RVAGLRQQIELKET HEELRRRIASS +AGTSTK
Sbjct: 421 AKLETENADLARALASVQWNLELEGKRVAGLRQQIELKETTHEELRRRIASSHQAGTSTK 480

Query: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540
           PLAFKGIEFELEIL+AEHSLITDKVL+LQEKGKKLEE+IEL+RK+MEEPTEVEVE+KRRL
Sbjct: 481 PLAFKGIEFELEILDAEHSLITDKVLKLQEKGKKLEENIELMRKDMEEPTEVEVEVKRRL 540

Query: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600
           GQMTDHLIQKQ QVEALSSEKATLLFRIEAVTRQLEE+KS++NTNDI+S+S RDLESGKW
Sbjct: 541 GQMTDHLIQKQAQVEALSSEKATLLFRIEAVTRQLEENKSVMNTNDISSSSSRDLESGKW 600

Query: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660
           +LSGSKLRPML+DKIHSGKKHLGSLIQQLDAIY AGMVFIRRNPAAKLWSVVYL+CLH W
Sbjct: 601 ELSGSKLRPMLQDKIHSGKKHLGSLIQQLDAIYGAGMVFIRRNPAAKLWSVVYLVCLHFW 660

Query: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           VLYI MSHS+VDTE+KSGAVISLENIN SLNM
Sbjct: 661 VLYILMSHSQVDTETKSGAVISLENINASLNM 689

BLAST of CmoCh10G003500 vs. ExPASy TrEMBL
Match: A0A6J1F667 (golgin candidate 2-like OS=Cucurbita moschata OX=3662 GN=LOC111441182 PE=4 SV=1)

HSP 1 Score: 1068.1 bits (2761), Expect = 1.5e-308
Identity = 593/692 (85.69%), Postives = 636/692 (91.91%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60
           MANWISSKLKAAESILQQIDQQAAESLKK ERPPAVDNLEA GKTGDILPLKDQLKKKNQ
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKDERPPAVDNLEATGKTGDILPLKDQLKKKNQ 60

Query: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120
            D DYHGK RSDLSLNVSRNQDNVISA+ K SPSSKPSTLTDSDWTELLG PNQPSTS A
Sbjct: 61  DDKDYHGKFRSDLSLNVSRNQDNVISASPKSSPSSKPSTLTDSDWTELLGTPNQPSTSAA 120

Query: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180
           +RSNG+ ++RGAKRD R+PSNAGS++SVLDFK+TQNNT SN+SVGE K+LNRK +DVEES
Sbjct: 121 ARSNGASSIRGAKRDGRKPSNAGSTVSVLDFKRTQNNTISNKSVGETKRLNRKASDVEES 180

Query: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240
           N+SV L +++RVDLK+D+NI HSEGRELDKK+AG NI+VEAKSVEK E GGNFDSKGFS 
Sbjct: 181 NVSVPLRTDTRVDLKSDQNITHSEGRELDKKEAGDNILVEAKSVEKKEFGGNFDSKGFSL 240

Query: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300
           +DS L IRN  SSET+PDT QA+RISD   IV   Q+HQ SG +GKHKSDE SRSSISD 
Sbjct: 241 KDSMLAIRNGHSSETMPDTGQAERISDTKIIVKAVQNHQGSGVSGKHKSDEFSRSSISDD 300

Query: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360
           VRKEWTGSSTS GSSGSDSDSGSASD+EIERE+EE +RRRQKILAEKAAAKA+EAIKE E
Sbjct: 301 VRKEWTGSSTSDGSSGSDSDSGSASDSEIEREREEKRRRRQKILAEKAAAKALEAIKEQE 360

Query: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420
           DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMME MEAVELEKQKHNETRREALA M
Sbjct: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMETMEAVELEKQKHNETRREALAIM 420

Query: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480
            KLETENADLA+TLASVQWNLELEGNRVAGLRQQIELKET HEELRRRIASS +AGTSTK
Sbjct: 421 AKLETENADLARTLASVQWNLELEGNRVAGLRQQIELKETTHEELRRRIASSHQAGTSTK 480

Query: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540
            L FKGIEFELEILEAEHSLITDKVLQLQEKG KLEE+IEL+R+EMEEPTEVEVELKRRL
Sbjct: 481 SLPFKGIEFELEILEAEHSLITDKVLQLQEKGGKLEENIELMRREMEEPTEVEVELKRRL 540

Query: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600
           GQMTDHLIQKQ QVEALSSEKATLLFRIEAV RQLEE+K MVNTN+I+STS RDLESGKW
Sbjct: 541 GQMTDHLIQKQAQVEALSSEKATLLFRIEAVMRQLEENKFMVNTNNISSTSSRDLESGKW 600

Query: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660
           +LSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIY AGMVFIRRNPAAKLWSVVYL+CLHLW
Sbjct: 601 ELSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYVAGMVFIRRNPAAKLWSVVYLVCLHLW 660

Query: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           VLYI MS S+VDTE+ SGAVISL+NIN SL+M
Sbjct: 661 VLYILMSRSQVDTETLSGAVISLDNINASLHM 692

BLAST of CmoCh10G003500 vs. ExPASy TrEMBL
Match: A0A6J1KMQ4 (golgin candidate 2-like OS=Cucurbita maxima OX=3661 GN=LOC111495566 PE=4 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 3.4e-305
Identity = 586/692 (84.68%), Postives = 633/692 (91.47%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60
           MANWISSKLKAAESILQQIDQQAAESLKK ERPP+V NLEA GKTGDILPLKDQLKKKNQ
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKDERPPSVGNLEATGKTGDILPLKDQLKKKNQ 60

Query: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120
            D DYHGK RSDLSLNVSRNQDNVISA+ K SPSSKPSTLTDSDWTELLG PNQPSTS A
Sbjct: 61  DDKDYHGKFRSDLSLNVSRNQDNVISASPKSSPSSKPSTLTDSDWTELLGTPNQPSTSAA 120

Query: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180
           +RSNG+ ++RGAKRD R+PSNAGS++S +DFK+TQNNT SN+SVGE K+LNRK +DVEES
Sbjct: 121 ARSNGASSIRGAKRDGRKPSNAGSTVSAMDFKRTQNNTISNKSVGETKRLNRKASDVEES 180

Query: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240
           N+SV L +++RVDLK+D+NIMHSEGRELDKK+AG NI+VEAKSVEK E GGNFDSKGFS 
Sbjct: 181 NVSVPLRTDTRVDLKSDQNIMHSEGRELDKKEAGDNILVEAKSVEKKECGGNFDSKGFSL 240

Query: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300
           +DS L IRN  SSET+PDT QA RISD   IV   Q+HQ SG +GKHKSDEVSRSSISD 
Sbjct: 241 KDSVLAIRNGHSSETMPDTGQADRISDTKIIVKAVQNHQGSGVSGKHKSDEVSRSSISDD 300

Query: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360
           VRKEWTGSSTS GSSGSDSDSGSASD+EIERE+EE +RRRQKILAEKAAAKA+EAIKE E
Sbjct: 301 VRKEWTGSSTSDGSSGSDSDSGSASDSEIEREREEKRRRRQKILAEKAAAKALEAIKEQE 360

Query: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420
           DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMME MEAVELEKQKHNETRREALA M
Sbjct: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMETMEAVELEKQKHNETRREALAIM 420

Query: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480
            KLETENADLA+TLASVQWNLELEGNRVAGLRQQIELKET HEELRRRIASS +AGTSTK
Sbjct: 421 AKLETENADLARTLASVQWNLELEGNRVAGLRQQIELKETTHEELRRRIASSHQAGTSTK 480

Query: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540
            L FKGIEFELEILEAE SLITDKVLQLQEKG+KLEE+IEL+R+EMEEPTEVE+ELK+RL
Sbjct: 481 SLPFKGIEFELEILEAELSLITDKVLQLQEKGEKLEENIELMRREMEEPTEVEIELKKRL 540

Query: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600
           GQMTDHLIQKQ QVEALSSEKATLLFR EAV RQLEE+K MVNTN+I+ST  RDLESGKW
Sbjct: 541 GQMTDHLIQKQAQVEALSSEKATLLFRTEAVMRQLEENKFMVNTNNISSTLSRDLESGKW 600

Query: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660
           +LSGSKLRPMLEDKIHSGKKHLGSLI+QLDAIY AGMVFIRRNPAAKLWSVVYL+CLHLW
Sbjct: 601 ELSGSKLRPMLEDKIHSGKKHLGSLIRQLDAIYVAGMVFIRRNPAAKLWSVVYLVCLHLW 660

Query: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           VLYI MS S+VDTE+ SGAVISL+NIN SLNM
Sbjct: 661 VLYILMSRSQVDTETLSGAVISLDNINASLNM 692

BLAST of CmoCh10G003500 vs. NCBI nr
Match: XP_022921865.1 (golgin candidate 2-like [Cucurbita moschata])

HSP 1 Score: 1259.2 bits (3257), Expect = 0.0e+00
Identity = 692/692 (100.00%), Postives = 692/692 (100.00%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60

Query: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120
           VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA
Sbjct: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120

Query: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180
           SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES
Sbjct: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180

Query: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240
           NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS
Sbjct: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240

Query: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300
           EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG
Sbjct: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300

Query: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360
           VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE
Sbjct: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360

Query: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420
           DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM
Sbjct: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420

Query: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480
           VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK
Sbjct: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480

Query: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540
           PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL
Sbjct: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540

Query: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600
           GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW
Sbjct: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600

Query: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660
           KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW
Sbjct: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660

Query: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           VLYIFMSHSRVDTESKSGAVISLENINTSLNM
Sbjct: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 692

BLAST of CmoCh10G003500 vs. NCBI nr
Match: KAG6589632.1 (Golgin candidate 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1253.0 bits (3241), Expect = 0.0e+00
Identity = 688/692 (99.42%), Postives = 692/692 (100.00%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60

Query: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120
           VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA
Sbjct: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120

Query: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180
           SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES
Sbjct: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180

Query: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240
           NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS
Sbjct: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240

Query: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300
           EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG
Sbjct: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300

Query: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360
           VRKEWTGSSTSGGSSGSDSDSGSASDAEIERE+EEIKRRRQKILAEKAAAKAMEAIKEHE
Sbjct: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREREEIKRRRQKILAEKAAAKAMEAIKEHE 360

Query: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420
           DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM
Sbjct: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420

Query: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480
           VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK
Sbjct: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480

Query: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540
           PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEE+IELVRKEMEEPTEVEVELKRRL
Sbjct: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEENIELVRKEMEEPTEVEVELKRRL 540

Query: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600
           GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDI+STSWRDLESGKW
Sbjct: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDISSTSWRDLESGKW 600

Query: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660
           KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW
Sbjct: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660

Query: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           VLYIFMSHS+VDTESKSGAVISLENINTSLNM
Sbjct: 661 VLYIFMSHSQVDTESKSGAVISLENINTSLNM 692

BLAST of CmoCh10G003500 vs. NCBI nr
Match: KAG7023320.1 (Golgin candidate 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1245.7 bits (3222), Expect = 0.0e+00
Identity = 683/692 (98.70%), Postives = 690/692 (99.71%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ
Sbjct: 68  MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKNQ 127

Query: 61  VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 120
           VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA
Sbjct: 128 VDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTSTA 187

Query: 121 SRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 180
           SRSNGSPTVRGAKRDVRRP+NAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES
Sbjct: 188 SRSNGSPTVRGAKRDVRRPTNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEES 247

Query: 181 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 240
           NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS
Sbjct: 248 NISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFSS 307

Query: 241 EDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 300
           EDSALTIRN QSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG
Sbjct: 308 EDSALTIRNGQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISDG 367

Query: 301 VRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEHE 360
           VRKEWTGSSTS GSSGSDSDSGSASDAEIERE+EEIKRRRQKILAEKAAAKAMEAIKEHE
Sbjct: 368 VRKEWTGSSTSSGSSGSDSDSGSASDAEIEREREEIKRRRQKILAEKAAAKAMEAIKEHE 427

Query: 361 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 420
           DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM
Sbjct: 428 DLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAKM 487

Query: 421 VKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 480
           VKLETENADLAKTLAS+QWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK
Sbjct: 488 VKLETENADLAKTLASLQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTSTK 547

Query: 481 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRRL 540
           PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEE+IELVRKEMEEPTEVEVELKRRL
Sbjct: 548 PLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEENIELVRKEMEEPTEVEVELKRRL 607

Query: 541 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGKW 600
           GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSM+NTNDI+STSWRDLESGKW
Sbjct: 608 GQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMMNTNDISSTSWRDLESGKW 667

Query: 601 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 660
           KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW
Sbjct: 668 KLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHLW 727

Query: 661 VLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           VLYIFMSHS+VDTESKSGAVISLENINTSLNM
Sbjct: 728 VLYIFMSHSQVDTESKSGAVISLENINTSLNM 759

BLAST of CmoCh10G003500 vs. NCBI nr
Match: XP_022987956.1 (golgin candidate 2-like [Cucurbita maxima])

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 678/693 (97.84%), Postives = 687/693 (99.13%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQL-KKKN 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQL KKKN
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKKN 60

Query: 61  QVDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTST 120
           QVDNDYHGKLRSDLSLNVSRNQDNVISAA KPSP+SKPSTLTDSDWTELLGIPNQPSTST
Sbjct: 61  QVDNDYHGKLRSDLSLNVSRNQDNVISAAPKPSPTSKPSTLTDSDWTELLGIPNQPSTST 120

Query: 121 ASRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEE 180
           ASRSNGSPTVRGAKRDVRRPSNAGS+MSVLDFKKTQNNT+SNRSVGERKKL RKT+DVEE
Sbjct: 121 ASRSNGSPTVRGAKRDVRRPSNAGSNMSVLDFKKTQNNTKSNRSVGERKKLTRKTSDVEE 180

Query: 181 SNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFS 240
           SNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFS
Sbjct: 181 SNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFS 240

Query: 241 SEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISD 300
           SEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISD
Sbjct: 241 SEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISD 300

Query: 301 GVRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEH 360
           GVRKEWTGSSTSGGSSG DSDS SASDAEIERE+EEIKRRRQKILAEKAAAKAMEAIKEH
Sbjct: 301 GVRKEWTGSSTSGGSSGLDSDSDSASDAEIEREREEIKRRRQKILAEKAAAKAMEAIKEH 360

Query: 361 EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK 420
           EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK
Sbjct: 361 EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK 420

Query: 421 MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST 480
           MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST
Sbjct: 421 MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST 480

Query: 481 KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRR 540
           KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEE IELVRKEMEEPTEVEVELKRR
Sbjct: 481 KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEESIELVRKEMEEPTEVEVELKRR 540

Query: 541 LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGK 600
           LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTN+I+STSWRDLESGK
Sbjct: 541 LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNNISSTSWRDLESGK 600

Query: 601 WKLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHL 660
           WKLSGSKL+PMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHL
Sbjct: 601 WKLSGSKLKPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHL 660

Query: 661 WVLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           WVLYIFMSHS+VDTESKSGAVISLENINTSLNM
Sbjct: 661 WVLYIFMSHSQVDTESKSGAVISLENINTSLNM 693

BLAST of CmoCh10G003500 vs. NCBI nr
Match: XP_023515724.1 (golgin candidate 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1226.5 bits (3172), Expect = 0.0e+00
Identity = 677/693 (97.69%), Postives = 686/693 (98.99%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQL-KKKN 60
           MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQL KKKN
Sbjct: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNLEAIGKTGDILPLKDQLKKKKN 60

Query: 61  QVDNDYHGKLRSDLSLNVSRNQDNVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTST 120
           QVDNDYHGKLRSDLSLNVSRNQ+NVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTST
Sbjct: 61  QVDNDYHGKLRSDLSLNVSRNQENVISAASKPSPSSKPSTLTDSDWTELLGIPNQPSTST 120

Query: 121 ASRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGERKKLNRKTNDVEE 180
           ASRSNGSPTVRGAKRDVRRPSNAGS+MSVLDFKKTQNNT+SNRS+GERKKL RKT+DVEE
Sbjct: 121 ASRSNGSPTVRGAKRDVRRPSNAGSNMSVLDFKKTQNNTKSNRSLGERKKLTRKTSDVEE 180

Query: 181 SNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEKNEIGGNFDSKGFS 240
           SNISVSLGSNSRV LKNDKNIMHSEGRELDKKDAGGNI VEAKSVEKNEI GNFDSKGFS
Sbjct: 181 SNISVSLGSNSRVALKNDKNIMHSEGRELDKKDAGGNISVEAKSVEKNEICGNFDSKGFS 240

Query: 241 SEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGKHKSDEVSRSSISD 300
           SE+SALTIRNSQSSETVPDTDQAKRISDMN IVTNAQSHQESGFTGKHKSDEVSRSSISD
Sbjct: 241 SENSALTIRNSQSSETVPDTDQAKRISDMNIIVTNAQSHQESGFTGKHKSDEVSRSSISD 300

Query: 301 GVRKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILAEKAAAKAMEAIKEH 360
           GVRKEWTGSSTSGGSSGSDSDSGSASDAEIERE+EEIKRRRQKILAEKAAAKAMEAIKEH
Sbjct: 301 GVRKEWTGSSTSGGSSGSDSDSGSASDAEIEREREEIKRRRQKILAEKAAAKAMEAIKEH 360

Query: 361 EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK 420
           EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK
Sbjct: 361 EDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELEKQKHNETRREALAK 420

Query: 421 MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST 480
           MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST
Sbjct: 421 MVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEELRRRIASSQEAGTST 480

Query: 481 KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRR 540
           KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRR
Sbjct: 481 KPLAFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRKEMEEPTEVEVELKRR 540

Query: 541 LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDITSTSWRDLESGK 600
           LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDI+STSWRDLESGK
Sbjct: 541 LGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNTNDISSTSWRDLESGK 600

Query: 601 WKLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNPAAKLWSVVYLICLHL 660
           WKLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIY AGMVFIRRNPAAKLWSVVYLICLHL
Sbjct: 601 WKLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYVAGMVFIRRNPAAKLWSVVYLICLHL 660

Query: 661 WVLYIFMSHSRVDTESKSGAVISLENINTSLNM 693
           WVLYIFMSHS+VDTESKSGAVISLENINTSLNM
Sbjct: 661 WVLYIFMSHSQVDTESKSGAVISLENINTSLNM 693

BLAST of CmoCh10G003500 vs. TAIR 10
Match: AT1G18190.1 (golgin candidate 2 )

HSP 1 Score: 486.9 bits (1252), Expect = 2.7e-137
Identity = 339/707 (47.95%), Postives = 456/707 (64.50%), Query Frame = 0

Query: 1   MANWISSKLKAAESILQQIDQQAAESLKKGERPPAVDNL-EAIGKTGDI-LPLKDQLKKK 60
           MANWISSKLKAAE+ILQQ+DQQAA+SL+K E+    D + E   K+G   + LKDQL+KK
Sbjct: 1   MANWISSKLKAAETILQQLDQQAADSLRKDEKSETHDEVFETSPKSGSSPVSLKDQLRKK 60

Query: 61  NQVDNDY-HGKLRSDLSLNVS--------RNQDNVISAASKPSPS--SKPSTLTDSDWTE 120
               +D   G  R+      S        R  D      S PS S     + LTD+DWTE
Sbjct: 61  TYEGSDSGSGSQRNSTEQKPSYLSSSKKVRKPDQSHERTSAPSQSLTQDNTKLTDNDWTE 120

Query: 121 LLGIPNQ-PSTSTASRSNGSPTVRGAKRDVRRPSNAGSSMSVLDFKKTQNNTRSNRSVGE 180
           LL  PNQ  STST+    G+  +RG K+D +R  N G +  V D KK+ ++   N     
Sbjct: 121 LLSTPNQRTSTSTSRSPGGTSAIRGLKKDGKRHGNLGKNPLVSDGKKSSSSNVVNSRGRP 180

Query: 181 RKKLNRKTNDVEESNISVSLGSNSRVDLKNDKNIMHSEGRELDKKDAGGNIVVEAKSVEK 240
           +K+ N++ +D E S       S S  D+KN              ++A  +I V +   E 
Sbjct: 181 QKQTNKEPSDKEVS-------SPSDADMKN--------------RNAPRDIFVNSTHKE- 240

Query: 241 NEIGGNFDSKGFSSEDSALTIRNSQSSETVPDTDQAKRISDMNTIVTNAQSHQESGFTGK 300
                    K  S +   L      ++ET+P      R +  +    + +  + S   GK
Sbjct: 241 -------SEKDVSGKTPPLDDSRRSANETLP------RETSPSVGKRDGRESRRSSVWGK 300

Query: 301 HKSDEVSRSSISDGV-RKEWTGSSTSGGSSGSDSDSGSASDAEIEREKEEIKRRRQKILA 360
              +EVS+S++SDG+ RKE   SS S   S SD +S S++D+E ER++EE +RRR+++ A
Sbjct: 301 QVREEVSQSNVSDGLTRKE---SSLSSDESESDYESDSSTDSERERQREERRRRRERVFA 360

Query: 361 EKAAAKAMEAIKEHEDLVARLEGEKQSLEKILEDRARKQAEEASELQTSMMEMMEAVELE 420
           EK A KA+  IKE E++VARLEGEK SLEKI+E+RA++QA+EA+ELQT+MME +EA +LE
Sbjct: 361 EKVATKAVAVIKERENMVARLEGEKLSLEKIVEERAKQQAQEAAELQTNMMETLEAADLE 420

Query: 421 KQKHNETRREALAKMVKLETENADLAKTLASVQWNLELEGNRVAGLRQQIELKETCHEEL 480
           KQKHN TR E L ++  LE ENA+L ++LA+ Q  LE + ++VA L+QQ+ELKE+  EEL
Sbjct: 421 KQKHNNTRMEVLTRLAGLEAENAELTRSLAAGQKKLETQIDQVAVLKQQVELKESTLEEL 480

Query: 481 RRRIASSQEAGTSTKPL-AFKGIEFELEILEAEHSLITDKVLQLQEKGKKLEEDIELVRK 540
           +R   +    GT+ K L   +G +FE ++LEAE SL+TDK+ +LQ+K  KLE DIE++RK
Sbjct: 481 KRNTFNIGGRGTTLKQLDTSRGDKFEHQMLEAEISLLTDKIGRLQDKATKLEADIEMMRK 540

Query: 541 EMEEPTEVEVELKRRLGQMTDHLIQKQVQVEALSSEKATLLFRIEAVTRQLEESKSMVNT 600
           E+EEPTEVE+ELKRRL Q+TDHLIQKQ QVEALSSEKAT+LFRIEAV+R +EE+K M   
Sbjct: 541 ELEEPTEVEIELKRRLNQLTDHLIQKQSQVEALSSEKATILFRIEAVSRLIEENKGM--- 600

Query: 601 NDITSTSWRDLESGKWKLSGSKLRPMLEDKIHSGKKHLGSLIQQLDAIYAAGMVFIRRNP 660
              T  S +DLE+G W+LSGSK +P  +DKI SGKKHLG L+ QL+AI+ +G VF+RRNP
Sbjct: 601 -SATEASSQDLEAGDWELSGSKFKPAFQDKIRSGKKHLGWLVMQLNAIFISGTVFLRRNP 660

Query: 661 AAKLWSVVYLICLHLWVLYIFMSHSRVDT--ESKSGAVISLENINTS 690
            AK+W+VVYL+CLHLWVLYI +SHS   +  E +SGAVISLEN + S
Sbjct: 661 TAKIWAVVYLVCLHLWVLYILLSHSDASSSGELRSGAVISLENFSNS 665