CmoCh09G002330 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh09G002330
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionheat stress transcription factor B-4-like
LocationCmo_Chr09: 1070417 .. 1071523 (-)
RNA-Seq ExpressionCmoCh09G002330
SyntenyCmoCh09G002330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGAACACAATGGCTCTAATGGTTGATAATTGCGAAGGAGTTTTGGTGTCCTTGGAGTCCCATAAGCCAATCCCAGCTCCCTTTCTCACTAAAACATACCAGCTCGTCGACGATCCCTCCACTGACCATATTGTCTCATGGGGCGAAGATGATACCACGTTCGTTGTTTGGCGTCCTCCTGAATTTGCTAGAGATCTCCTTCCTAATTATTTCAAGCACAACAATTTCTCTAGCTTTGTCCGCCAGCTCAACACCTATGTAAGAATATCATCAATCAAGAATTCAAACATTCTCTCTGTTTCTTTCAATCAGCTTATTTTTCTTGTTCTGCTTTTAGGGGTTCAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAACGACTTCTTCAGAAAAGGAGATAAACATTTGTTATGTGAAATCCATAGACGGAAAACCGCTCAGCCACAACACCAACAATCTCTTTCTCCACTTGCTCTCAACAATCCAGGGTTTTACCACTTCCATGCCCGACCCAGCATCTCGCCCTCCGACTCTGACGACCAAAATAATTGGTGCGACTCGCCGCCGCTCTCCTCCTCTGGCACTAACAACAACAACAACTCTGTCTCCGCCCTGTCGGAGGACAACGAGCGGCTTCGGCGAAGCAATAACATGCTGATGTCGGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTATTTCGTTCAAAACCACGTGAAGCCCGTCGCCCCAAGTAATTCATATCAATACTCAACCTTTCCAGTGGTGCAACGGCAGCCGAACCACCATCTTGTCGGGTACAATAATCCGACAAATGCGAAACAAGTTTCGGGTCAGACCCATTTGGTGACTGCAACTCCAAATAATAATAATAACAACAACAAAAGCTTCGTGAGGATTGTAGAAGAACAACAAAAAACGAAGCTTTTTGGAGTGGCGATTCATTCGAAGAAACGGCTGCATCCGGAGTATGCTTCTAATAATATTGGGAAAGAGAACAACAACAAGGCTCGATTTGTGTTGGAGAAAGATGATTTAGGGCTGAATCTCATGCCCCCTTCCGCTTGTTAG

mRNA sequence

TCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGAACACAATGGCTCTAATGGTTGATAATTGCGAAGGAGTTTTGGTGTCCTTGGAGTCCCATAAGCCAATCCCAGCTCCCTTTCTCACTAAAACATACCAGCTCGTCGACGATCCCTCCACTGACCATATTGTCTCATGGGGCGAAGATGATACCACGTTCGTTGTTTGGCGTCCTCCTGAATTTGCTAGAGATCTCCTTCCTAATTATTTCAAGCACAACAATTTCTCTAGCTTTGTCCGCCAGCTCAACACCTATGGGTTCAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAACGACTTCTTCAGAAAAGGAGATAAACATTTGTTATGTGAAATCCATAGACGGAAAACCGCTCAGCCACAACACCAACAATCTCTTTCTCCACTTGCTCTCAACAATCCAGGGTTTTACCACTTCCATGCCCGACCCAGCATCTCGCCCTCCGACTCTGACGACCAAAATAATTGGTGCGACTCGCCGCCGCTCTCCTCCTCTGGCACTAACAACAACAACAACTCTGTCTCCGCCCTGTCGGAGGACAACGAGCGGCTTCGGCGAAGCAATAACATGCTGATGTCGGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTATTTCGTTCAAAACCACGTGAAGCCCGTCGCCCCAAGTAATTCATATCAATACTCAACCTTTCCAGTGGTGCAACGGCAGCCGAACCACCATCTTGTCGGGTACAATAATCCGACAAATGCGAAACAAGTTTCGGGTCAGACCCATTTGGTGACTGCAACTCCAAATAATAATAATAACAACAACAAAAGCTTCGTGAGGATTGTAGAAGAACAACAAAAAACGAAGCTTTTTGGAGTGGCGATTCATTCGAAGAAACGGCTGCATCCGGAGTATGCTTCTAATAATATTGGGAAAGAGAACAACAACAAGGCTCGATTTGTGTTGGAGAAAGATGATTTAGGGCTGAATCTCATGCCCCCTTCCGCTTGTTAG

Coding sequence (CDS)

ATGGCTCTAATGGTTGATAATTGCGAAGGAGTTTTGGTGTCCTTGGAGTCCCATAAGCCAATCCCAGCTCCCTTTCTCACTAAAACATACCAGCTCGTCGACGATCCCTCCACTGACCATATTGTCTCATGGGGCGAAGATGATACCACGTTCGTTGTTTGGCGTCCTCCTGAATTTGCTAGAGATCTCCTTCCTAATTATTTCAAGCACAACAATTTCTCTAGCTTTGTCCGCCAGCTCAACACCTATGGGTTCAGAAAAATTGTGCCGGACAGATGGGAATTTGCGAACGACTTCTTCAGAAAAGGAGATAAACATTTGTTATGTGAAATCCATAGACGGAAAACCGCTCAGCCACAACACCAACAATCTCTTTCTCCACTTGCTCTCAACAATCCAGGGTTTTACCACTTCCATGCCCGACCCAGCATCTCGCCCTCCGACTCTGACGACCAAAATAATTGGTGCGACTCGCCGCCGCTCTCCTCCTCTGGCACTAACAACAACAACAACTCTGTCTCCGCCCTGTCGGAGGACAACGAGCGGCTTCGGCGAAGCAATAACATGCTGATGTCGGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTATTTCGTTCAAAACCACGTGAAGCCCGTCGCCCCAAGTAATTCATATCAATACTCAACCTTTCCAGTGGTGCAACGGCAGCCGAACCACCATCTTGTCGGGTACAATAATCCGACAAATGCGAAACAAGTTTCGGGTCAGACCCATTTGGTGACTGCAACTCCAAATAATAATAATAACAACAACAAAAGCTTCGTGAGGATTGTAGAAGAACAACAAAAAACGAAGCTTTTTGGAGTGGCGATTCATTCGAAGAAACGGCTGCATCCGGAGTATGCTTCTAATAATATTGGGAAAGAGAACAACAACAAGGCTCGATTTGTGTTGGAGAAAGATGATTTAGGGCTGAATCTCATGCCCCCTTCCGCTTGTTAG

Protein sequence

MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC
Homology
BLAST of CmoCh09G002330 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.3e-85
Identity = 187/365 (51.23%), Postives = 233/365 (63.84%), Query Frame = 0

Query: 1   MALMVDNCEG---------VLVSLESH-KPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA+MV+N  G         + + +E   K +PAPFLTKTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FF++G+KHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQPQHQQSLSPLALNNPGFYHFHARPSISPSDS-------------DDQNNWCD 180
           IHRRKT+Q   QQ       ++P   H HA P I  S               ++ + WCD
Sbjct: 121 IHRRKTSQMIPQQ-------HSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTPEEDHYWCD 180

Query: 181 SPPLSSSGT----NNNNNSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVK 240
             P S         +    V+ALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVK
Sbjct: 181 DSPPSRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVK 240

Query: 241 PVAPSNSYQY-STFPVVQRQPNHHLVGYNNPT----------NAKQVSGQTHLVTATPNN 300
           PVAPSN+  Y S+F   Q+Q     + Y N            N+   + Q+ +     ++
Sbjct: 241 PVAPSNNSSYLSSFLQKQQQQQPPTLDYYNTATVNATNLNALNSSPPTSQSSITVLEDDH 300

Query: 301 NNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLN 328
            N++++S +R      KTKLFGV++ S K+    ++      +  +K R VL++ DL LN
Sbjct: 301 TNHHDQSNMR------KTKLFGVSLPSSKKRSHHFS------DQTSKTRLVLDQSDLALN 346

BLAST of CmoCh09G002330 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.6e-71
Identity = 159/316 (50.32%), Postives = 191/316 (60.44%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLE----------SHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA +V+ C  ++VS+E          + KP+PAPFLTKTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFAN+FFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQP----------QHQQSLSPLAL-NNPGFYHFHARPSISPSDSDDQNNWCDSP 180
           IHRRK++QP           H   L+P +L   P  YH H      P+ +        + 
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHHHLNPFSLPPPPPAYHHHHLIQEEPATT--------AH 180

Query: 181 PLSSSGTNNNNNSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSN 240
              +       + ++ALSEDN +LRR N++L+SELAHMKKLYNDIIYF+QNHV PV  + 
Sbjct: 181 CTVAGDGGEGGDFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHVAPVTTTT 240

Query: 241 SYQYSTFPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQK 295
           +   ST         HHL    +     ++    H     P              +    
Sbjct: 241 TTPSST---AMAAAQHHLPAAAS-CRLMELDSPDHSPPPPPPK--------TPATDGGDT 296

BLAST of CmoCh09G002330 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 7.6e-66
Identity = 157/331 (47.43%), Postives = 198/331 (59.82%), Query Frame = 0

Query: 1   MALMVDNCEG-VLVSLE-SH----------KPIPAPFLTKTYQLVDDPSTDHIVSWGEDD 60
           MA +V+ C G ++VS+E SH             PAPFL+KTYQLVDDPSTD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 61  TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLL 120
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFAN+FFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 121 CEIHRRKTAQ----------PQHQQSLSPLALNNP--------------GFYHFHARPSI 180
            EIHRRK++           P HQ    PL+L +P                YHF      
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQH--YPLSLFSPPTTPRSPPVGAAAAAAYHF------ 180

Query: 181 SPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDNERLRRSNNMLMSELAHMKKLYNDI 240
                  Q  +C SP   + G     + ++ALSEDN +LRR N++L+SELAHM+KLYNDI
Sbjct: 181 -------QEEYCSSPADYAGG---GGDLLAALSEDNRQLRRRNSLLLSELAHMRKLYNDI 240

Query: 241 IYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNN 293
           IYF+QNHV+PVAP      ++  +V+  P+               + +     A+P+ +N
Sbjct: 241 IYFLQNHVEPVAPPPLAAATSCRLVELGPS---------------TTERRRCAASPSGDN 288

BLAST of CmoCh09G002330 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.6e-63
Identity = 166/381 (43.57%), Postives = 204/381 (53.54%), Query Frame = 0

Query: 7   NCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDD-TTFVVWRPPEFARDLLP 66
           +CE    +  + K +PAPFLTKTYQLVDDP+TDHIVSWG+D  +TFVVWRPPEFARD+LP
Sbjct: 9   DCEAAAAA--AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILP 68

Query: 67  NYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQHQQSL 126
           NYFKHNNFSSFVRQLNTYGFRK+VP+RWEFAN+FFRKG+K LL EIHRRKT+        
Sbjct: 69  NYFKHNNFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTASPS 128

Query: 127 SPLALNNPGFYHFHARPSISPSD------SDD-------------QNNW----------- 186
            P     P F  FH  P ++ +        DD             Q +W           
Sbjct: 129 PPPFFAPPHFPLFH-HPGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRL 188

Query: 187 ------CDSPPLSSSGTNNNNNSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQ 246
                   SP  + +G      + + L E+NERLRRSN  L+ ELAHM+KLYNDIIYFVQ
Sbjct: 189 LALGGPAPSPSSAEAGGAGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDIIYFVQ 248

Query: 247 NHVKPVAPSNS---------YQYSTFPVVQRQPNH---HLVGYNNPTNAKQVS---GQTH 306
           NHV+PVAPS +          Q    P      N+        ++ T A++ S    Q H
Sbjct: 249 NHVRPVAPSPAAAAFLQGLGMQARKKPAAANVLNNSGGSTTSSSSLTIAEEPSPPPQQQH 308

Query: 307 LVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIH-------SKKRLHPEYASNNIGKENN 329
           L          N+ +          TKLFGV +        SK+   PE    +      
Sbjct: 309 LAGEKSGGEAGNSSA----ARSSAPTKLFGVHLSAAPCGAGSKRASSPE---EHPPTSPA 368

BLAST of CmoCh09G002330 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.7e-57
Identity = 123/234 (52.56%), Postives = 154/234 (65.81%), Query Frame = 0

Query: 21  IPAPFLTKTYQLVDDPSTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHNNFSS 80
           +PAPFLTKTYQLVDDP+TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+NFSS
Sbjct: 22  VPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHSNFSS 81

Query: 81  FVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQHQQSLSPLALNNPGF 140
           FVRQLNTYGFRK+VP+RWEFAN+FFRKG+K LLCEIHRRK+A      +  P     P F
Sbjct: 82  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSA----AATWPPFPPPPPPF 141

Query: 141 Y---HFHARPSISPSD------------SDDQNNWCDSPPLSSSGTNNNNNSV------- 200
           +   HF A       D            + ++ +W +S  L  + ++   + +       
Sbjct: 142 FAPRHFAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPA 201

Query: 201 ---------SALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPS 219
                     AL ++N RL R N  L+ ELAHM+KLY+DIIYFVQNHV+PVAPS
Sbjct: 202 RRAAATPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPS 251

BLAST of CmoCh09G002330 vs. ExPASy TrEMBL
Match: A0A6J1F8Q8 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111443150 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.0e-190
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120

Query: 121 HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDN 180
           HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDN
Sbjct: 121 HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDN 180

Query: 181 ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGY 240
           ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGY
Sbjct: 181 ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGY 240

Query: 241 NNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASN 300
           NNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASN
Sbjct: 241 NNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASN 300

Query: 301 NIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           NIGKENNNKARFVLEKDDLGLNLMPPSAC
Sbjct: 301 NIGKENNNKARFVLEKDDLGLNLMPPSAC 329

BLAST of CmoCh09G002330 vs. ExPASy TrEMBL
Match: A0A6J1IFM1 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111476507 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.6e-183
Identity = 319/334 (95.51%), Postives = 323/334 (96.71%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMVDNCEGVLVSLE HKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLEPHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120

Query: 121 HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNN-----NSVSA 180
           H QSLSPLALNNPGFYHFH R SISPSDSDDQNNWCDSPPLSSSGTNNNN     NSV+A
Sbjct: 121 HHQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSSSGTNNNNNNNYSNSVTA 180

Query: 181 LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNH 240
           LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNH
Sbjct: 181 LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNH 240

Query: 241 HLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHP 300
           HLVGYNNPTNAK+VSGQTHLVT TPNNNN++NKS VRIVEEQQKTKLFGVAIHSKKRLHP
Sbjct: 241 HLVGYNNPTNAKRVSGQTHLVTGTPNNNNDSNKSLVRIVEEQQKTKLFGVAIHSKKRLHP 300

Query: 301 EYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           EYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC
Sbjct: 301 EYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 334

BLAST of CmoCh09G002330 vs. ExPASy TrEMBL
Match: A0A6J1FJH2 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111445920 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 4.1e-155
Identity = 287/341 (84.16%), Postives = 303/341 (88.86%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 ----QHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNN-NNSVS 180
               QH QS SPL L N GFYH+ AR SISPSDSDD  NWCDSPPLSS+  NNN NNSV+
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNNNFNNSVT 180

Query: 181 ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST------FPV 240
           ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST      FPV
Sbjct: 181 ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDGFPV 240

Query: 241 VQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIH 300
            QRQPNHHL+GY +PTNAKQVSGQTH V  TPNNNN ++KSFV IVEE  KTKLFGV++ 
Sbjct: 241 GQRQPNHHLLGY-HPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEE-PKTKLFGVSLQ 300

Query: 301 SKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           SKKR+HPEY SNNI KE NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 SKKRVHPEYGSNNIVKE-NNKARLVLEKDDLGLNLMPPSAC 337

BLAST of CmoCh09G002330 vs. ExPASy TrEMBL
Match: A0A6J1IW73 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111481146 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.9e-155
Identity = 289/344 (84.01%), Postives = 304/344 (88.37%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 ----QHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNN--NNSV 180
               QH QS SPL L N GFYH+ AR SISPSDSDD  NWCDSPPLSS+  NNN  NNSV
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNNNVFNNSV 180

Query: 181 SALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST------FP 240
           +ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST      FP
Sbjct: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDGFP 240

Query: 241 VVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNN--NKSFVRIVEEQQKTKLFGV 300
           V QRQPNHHL+GY +PTNAKQVSGQTH V  TPNNNNNN  +KSFV IVEE  KTKLFGV
Sbjct: 241 VGQRQPNHHLLGY-HPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEE-PKTKLFGV 300

Query: 301 AIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           ++ SKKR+HPEY SNNI KE NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 SLQSKKRVHPEYGSNNIAKE-NNKARLVLEKDDLGLNLMPPSAC 340

BLAST of CmoCh09G002330 vs. ExPASy TrEMBL
Match: A0A5A7VAY1 (Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003260 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 5.2e-134
Identity = 268/349 (76.79%), Postives = 283/349 (81.09%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+D+C+GVL+SL+SHK IPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDSCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 ----QHQQSLSPLALNNPGFYHF-HARPSISPSDSDDQNN-WCDSPPLSSSGTNNNNNSV 180
               QH QSLSPL   NPGFYHF  AR SISPSDSDDQNN WCDSP   S   NNNNNSV
Sbjct: 121 VTVNQHHQSLSPL---NPGFYHFPTARLSISPSDSDDQNNYWCDSP---SPNNNNNNNSV 180

Query: 181 SALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST-------- 240
           +ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST        
Sbjct: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSTSLLSDG 240

Query: 241 FPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQ-----KT 300
           FPVV RQPNH+        +  QVS Q        NN     KSFV I+EE+Q     KT
Sbjct: 241 FPVV-RQPNHY-------HHHHQVSSQIR------NNTTVGTKSFVTILEEEQQQQQPKT 300

Query: 301 KLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           KLFGVAI SKKRLHPEY +++    NNNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 KLFGVAIQSKKRLHPEYGNSH--SNNNNKARLVLEKDDLGLNLMPPSAC 327

BLAST of CmoCh09G002330 vs. NCBI nr
Match: XP_022936594.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >KAG6591386.1 Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 675.6 bits (1742), Expect = 2.1e-190
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120

Query: 121 HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDN 180
           HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDN
Sbjct: 121 HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDN 180

Query: 181 ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGY 240
           ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGY
Sbjct: 181 ERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHLVGY 240

Query: 241 NNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASN 300
           NNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASN
Sbjct: 241 NNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASN 300

Query: 301 NIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           NIGKENNNKARFVLEKDDLGLNLMPPSAC
Sbjct: 301 NIGKENNNKARFVLEKDDLGLNLMPPSAC 329

BLAST of CmoCh09G002330 vs. NCBI nr
Match: XP_023536624.1 (heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.6 bits (1677), Expect = 7.3e-183
Identity = 320/332 (96.39%), Postives = 322/332 (96.99%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMVDNCEGVLVSL+SHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLDSHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120

Query: 121 HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGT---NNNNNSVSALS 180
             QSLSPLALNNPGFYHFH R SISPSDSDDQNNWCDSPPLSSSGT   NNNNNSV+ALS
Sbjct: 121 LHQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSSSGTNNNNNNNNSVTALS 180

Query: 181 EDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHL 240
           EDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHL
Sbjct: 181 EDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNHHL 240

Query: 241 VGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEY 300
           VGYNNPTNAKQVSGQTHLVT  PNNNN NNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEY
Sbjct: 241 VGYNNPTNAKQVSGQTHLVTGIPNNNNKNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEY 300

Query: 301 ASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           ASNNIGKENNNKARFVLEKDDLGLNLMPPSAC
Sbjct: 301 ASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 332

BLAST of CmoCh09G002330 vs. NCBI nr
Match: XP_022975965.1 (heat stress transcription factor B-4-like [Cucurbita maxima] >XP_022975966.1 heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 650.2 bits (1676), Expect = 9.5e-183
Identity = 319/334 (95.51%), Postives = 323/334 (96.71%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMVDNCEGVLVSLE HKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLEPHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120

Query: 121 HQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNNN-----NSVSA 180
           H QSLSPLALNNPGFYHFH R SISPSDSDDQNNWCDSPPLSSSGTNNNN     NSV+A
Sbjct: 121 HHQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSSSGTNNNNNNNYSNSVTA 180

Query: 181 LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNH 240
           LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNH
Sbjct: 181 LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVVQRQPNH 240

Query: 241 HLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHP 300
           HLVGYNNPTNAK+VSGQTHLVT TPNNNN++NKS VRIVEEQQKTKLFGVAIHSKKRLHP
Sbjct: 241 HLVGYNNPTNAKRVSGQTHLVTGTPNNNNDSNKSLVRIVEEQQKTKLFGVAIHSKKRLHP 300

Query: 301 EYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           EYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC
Sbjct: 301 EYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 334

BLAST of CmoCh09G002330 vs. NCBI nr
Match: XP_022940239.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.4 bits (1435), Expect = 8.4e-155
Identity = 287/341 (84.16%), Postives = 303/341 (88.86%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 ----QHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNN-NNSVS 180
               QH QS SPL L N GFYH+ AR SISPSDSDD  NWCDSPPLSS+  NNN NNSV+
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNNNFNNSVT 180

Query: 181 ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST------FPV 240
           ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST      FPV
Sbjct: 181 ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDGFPV 240

Query: 241 VQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNNNKSFVRIVEEQQKTKLFGVAIH 300
            QRQPNHHL+GY +PTNAKQVSGQTH V  TPNNNN ++KSFV IVEE  KTKLFGV++ 
Sbjct: 241 GQRQPNHHLLGY-HPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEE-PKTKLFGVSLQ 300

Query: 301 SKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           SKKR+HPEY SNNI KE NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 SKKRVHPEYGSNNIVKE-NNKARLVLEKDDLGLNLMPPSAC 337

BLAST of CmoCh09G002330 vs. NCBI nr
Match: XP_022982267.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 556.6 bits (1433), Expect = 1.4e-154
Identity = 289/344 (84.01%), Postives = 304/344 (88.37%), Query Frame = 0

Query: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 ----QHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSSSGTNNN--NNSV 180
               QH QS SPL L N GFYH+ AR SISPSDSDD  NWCDSPPLSS+  NNN  NNSV
Sbjct: 121 VTVNQHHQSYSPLGL-NAGFYHYPARVSISPSDSDDNPNWCDSPPLSSNNNNNNVFNNSV 180

Query: 181 SALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST------FP 240
           +ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST      FP
Sbjct: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDGFP 240

Query: 241 VVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNN--NKSFVRIVEEQQKTKLFGV 300
           V QRQPNHHL+GY +PTNAKQVSGQTH V  TPNNNNNN  +KSFV IVEE  KTKLFGV
Sbjct: 241 VGQRQPNHHLLGY-HPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEE-PKTKLFGV 300

Query: 301 AIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSAC 330
           ++ SKKR+HPEY SNNI KE NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 SLQSKKRVHPEYGSNNIAKE-NNKARLVLEKDDLGLNLMPPSAC 340

BLAST of CmoCh09G002330 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 316.6 bits (810), Expect = 2.3e-86
Identity = 187/365 (51.23%), Postives = 233/365 (63.84%), Query Frame = 0

Query: 1   MALMVDNCEG---------VLVSLESH-KPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA+MV+N  G         + + +E   K +PAPFLTKTYQLVDDP+TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FF++G+KHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQPQHQQSLSPLALNNPGFYHFHARPSISPSDS-------------DDQNNWCD 180
           IHRRKT+Q   QQ       ++P   H HA P I  S               ++ + WCD
Sbjct: 121 IHRRKTSQMIPQQ-------HSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTPEEDHYWCD 180

Query: 181 SPPLSSSGT----NNNNNSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVK 240
             P S         +    V+ALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVK
Sbjct: 181 DSPPSRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVK 240

Query: 241 PVAPSNSYQY-STFPVVQRQPNHHLVGYNNPT----------NAKQVSGQTHLVTATPNN 300
           PVAPSN+  Y S+F   Q+Q     + Y N            N+   + Q+ +     ++
Sbjct: 241 PVAPSNNSSYLSSFLQKQQQQQPPTLDYYNTATVNATNLNALNSSPPTSQSSITVLEDDH 300

Query: 301 NNNNNKSFVRIVEEQQKTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLN 328
            N++++S +R      KTKLFGV++ S K+    ++      +  +K R VL++ DL LN
Sbjct: 301 TNHHDQSNMR------KTKLFGVSLPSSKKRSHHFS------DQTSKTRLVLDQSDLALN 346

BLAST of CmoCh09G002330 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 101/198 (51.01%), Postives = 130/198 (65.66%), Query Frame = 0

Query: 17  SHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + + +PAPFL+KTYQLVDD STD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQHQQSLSPLALNNPGFY 136
           +RQLNTYGFRK VPD+WEFAND+FR+G + LL +I RRK+           +  +  G  
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKS-----------VIASTAGKC 126

Query: 137 HFHARPSISPS-DSDDQNNWCDSPPLSSSGTNNNNNSVSALSEDNERLRRSNNMLMSELA 196
                PS S S   DD  +   S P SS    +  N V+ LS +NE+L+R NN L SELA
Sbjct: 127 VVVGSPSESNSGGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSSELA 186

Query: 197 HMKKLYNDIIYFVQNHVK 214
             KK  ++++ F+  H+K
Sbjct: 187 AAKKQRDELVTFLTGHLK 193

BLAST of CmoCh09G002330 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.2e-44
Identity = 101/218 (46.33%), Postives = 132/218 (60.55%), Query Frame = 0

Query: 16  ESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           +S + IP PFLTKTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQHQQSLSPLA------ 135
           FVRQLNTYGFRK+VPDRWEF+ND F++G+K LL +I RRK +QP    + +  A      
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 136 ----LNNPGFYHFHARPSISPSDS-DDQNNWCDSPPLSSSGT------------NNNNNS 195
                  P   H      +SPS+S ++Q    +S P +++                +  +
Sbjct: 171 AVTVAAVPVVAHI-----VSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTT 230

Query: 196 VSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQN 211
              L E+NERLR+ N  L  E+  +K LY +I   + N
Sbjct: 231 APELVEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

BLAST of CmoCh09G002330 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 176.4 bits (446), Expect = 3.8e-44
Identity = 98/212 (46.23%), Postives = 130/212 (61.32%), Query Frame = 0

Query: 17  SHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S + IP PFLTKT+ LV+D S D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQHQQSLSPLALNNPGFY 136
           VRQLNTYGF+K+VPDRWEF+NDFF++G+K LL EI RRK     HQ  ++P +       
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKIT-TTHQTVVAPSSEQR---- 135

Query: 137 HFHARPSISPSDSDDQNN---WCDSPPLS----SSGTNNNNNSVSALSEDNERLRRSNNM 196
             +    +SPS+S + NN      S P S     + T  N      L E+NE+LR  N  
Sbjct: 136 --NQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEKLRSQNIQ 195

Query: 197 LMSELAHMKKLYNDIIYFVQNHVKPVAPSNSY 222
           L  EL  MK + ++I   + N+V       SY
Sbjct: 196 LNRELTQMKSICDNIYSLMSNYVGSQPTDRSY 220

BLAST of CmoCh09G002330 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 157.9 bits (398), Expect = 1.4e-38
Identity = 90/186 (48.39%), Postives = 109/186 (58.60%), Query Frame = 0

Query: 22  PAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLN 81
           P PFL+KTY +VDD +TD IVSW  ++ +F+VW+PPEFARDLLP  FKHNNFSSFVRQLN
Sbjct: 35  PPPFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSFVRQLN 94

Query: 82  TYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQHQQSLSPLALNNPGFYHFHAR 141
           TYGFRK+ PDRWEFAN+ F +G KHLL  I RRK A  Q Q                H R
Sbjct: 95  TYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQGQG---------------HQR 154

Query: 142 PSISPSDSDDQNNWCDSPPLSSSGTNNNNNSVSA--------LSEDNERLRRSNNMLMSE 200
                                S  +N  N+SVSA        L E+ ERL+R  N+LM E
Sbjct: 155 ---------------------SQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQE 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6353.3e-8551.23Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ04.6e-7150.32Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX87.6e-6647.43Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U941.6e-6343.57Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R81.7e-5752.56Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
A0A6J1F8Q81.0e-190100.00heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IFM14.6e-18395.51heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FJH24.1e-15584.16heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IW736.9e-15584.01heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A5A7VAY15.2e-13476.79Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
XP_022936594.12.1e-190100.00heat stress transcription factor B-4-like [Cucurbita moschata] >KAG6591386.1 Hea... [more]
XP_023536624.17.3e-18396.39heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo][more]
XP_022975965.19.5e-18395.51heat stress transcription factor B-4-like [Cucurbita maxima] >XP_022975966.1 hea... [more]
XP_022940239.18.4e-15584.16heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 h... [more]
XP_022982267.11.4e-15484.01heat stress transcription factor B-4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G46264.12.3e-8651.23heat shock transcription factor B4 [more]
AT4G36990.12.8e-4751.01heat shock factor 4 [more]
AT4G11660.12.2e-4446.33winged-helix DNA-binding transcription factor family protein [more]
AT5G62020.13.8e-4446.23heat shock transcription factor B2A [more]
AT1G32330.11.4e-3848.39heat shock transcription factor A1D [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 173..193
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..181
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..177
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 13..327
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 76..88
score: 84.62
coord: 25..48
score: 47.38
coord: 63..75
score: 78.68
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 5.5E-60
score: 215.3
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 2.5E-32
score: 111.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 18..116
e-value: 1.7E-37
score: 130.1
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 13..327
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh09G002330.1CmoCh09G002330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding