CmoCh08G012630 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G012630
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pectin lyase-like superfamily protein
Location	Cmo_Chr08: 7955491 .. 7957375 (+)
RNA-Seq Expression	CmoCh08G012630
Synteny	CmoCh08G012630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTCAATCGAGTTCCTGCTGTGATGCTTTGGATCACGGCCATTTTGGTGGCGGAGAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGGAAGACTCGGTTCCTTCAACTCCAGCTGACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGATGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTCAGCCACATTTTTTTAGAATTTGAATCTCTTGTTGTTCGTGTTGAAGTTGGAACACTTATAAGTAAACCTGTTAATGCAACAAATCAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTCGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGTACCTACATTGTCCTCTTAATATCTCTTGAATTTCTCTGTTTATTTCAGTTTCAACTTCCTATCCAAGGCTTAATTGTCCTTTTTATTTTAGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGGTAAGAAGGAAACCCACCTTTTTGCCCACCAATTCAACCAACTTATATGAATAAAACATGCTGACTGGTTCTGATATCTGGGGTTTTCAGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGACTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGAGAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCACATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGGTACGAAACTGAAATAATTGATTGTTCTTGTCCACTCATTTTTATCAACATCTTGGCTGATCTATGATTTGGAAACATAGGTGATGACTGCATTTCAATCGGGCCCGGCTGTGCCAACGTAGACATTGAAGGCGTCACTTGTGGGCCGAGTCATGGAATCAGGTACAAACAACGTCGATTGCAATCTCCATTTATTTGTTTATCACTAGTCAACGCAGTGAATAATTCATTGTTGTATGTAGCAGCATTGGGAGTCTGGGAGTCCACAATTCACAGGCATGCGTTTCCAACATAACAGTGAGAAATGCAGTGATTAGGGAATCCGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCTGGTACAGTGAGCGACATCTTATTCGAAAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAAGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACAAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGCTGA

mRNA sequence

ATGGAGTTCAATCGAGTTCCTGCTGTGATGCTTTGGATCACGGCCATTTTGGTGGCGGAGAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGGAAGACTCGGTTCCTTCAACTCCAGCTGACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGATGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTCAGCCACATTTTTTTAGAATTTGAATCTCTTGTTGTTCGTGTTGAAGTTGGAACACTTATAAGTAAACCTGTTAATGCAACAAATCAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTCGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGACTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGAGAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCACATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGGTGATGACTGCATTTCAATCGGGCCCGGCTGTGCCAACGTAGACATTGAAGGCGTCACTTGTGGGCCGAGTCATGGAATCAGGTACAAACAACGTCGATTGCAATCTCCATTTATTTGTTTATCACTAGTCAACGCAGTGAATAATTCATTGTTCATTGGGAGTCTGGGAGTCCACAATTCACAGGCATGCGTTTCCAACATAACAGTGAGAAATGCAGTGATTAGGGAATCCGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCTGGTACAGTGAGCGACATCTTATTCGAAAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAAGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACAAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGCTGA

Coding sequence (CDS)

ATGGAGTTCAATCGAGTTCCTGCTGTGATGCTTTGGATCACGGCCATTTTGGTGGCGGAGAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGGAAGACTCGGTTCCTTCAACTCCAGCTGACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGATGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTCAGCCACATTTTTTTAGAATTTGAATCTCTTGTTGTTCGTGTTGAAGTTGGAACACTTATAAGTAAACCTGTTAATGCAACAAATCAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTCGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGACTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGAGAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCACATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGGTGATGACTGCATTTCAATCGGGCCCGGCTGTGCCAACGTAGACATTGAAGGCGTCACTTGTGGGCCGAGTCATGGAATCAGGTACAAACAACGTCGATTGCAATCTCCATTTATTTGTTTATCACTAGTCAACGCAGTGAATAATTCATTGTTCATTGGGAGTCTGGGAGTCCACAATTCACAGGCATGCGTTTCCAACATAACAGTGAGAAATGCAGTGATTAGGGAATCCGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCTGGTACAGTGAGCGACATCTTATTCGAAAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAAGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACAAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGCTGA

Protein sequence

MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC

Homology

[Close]

BLAST of CmoCh08G012630 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.2e-190
Identity = 335/534 (62.73%), Postives = 392/534 (73.41%), Query Frame = 0


Query: 9   VMLWITAILVAENLSNV-QGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADPA 68
           ++++I + ++  + +NV + RYH+HKG+          +  P+  PPP    P   A+P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH--------GVTHPLPPPPPP---PLETANPP 67

Query: 69  PSVPSDPYPDD---PGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLV 128
             VPSDPYP     PG++ S CVFDVT++GAVGDG  DDTAAF+ AWKAACAVES  +L 
Sbjct: 68  DQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLA 127

Query: 129 PSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLM 188
           P    FKITSTIFSGPCKPGLVF                              Q+DG LM
Sbjct: 128 PEGGVFKITSTIFSGPCKPGLVF------------------------------QLDGVLM 187

Query: 189 PPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGP 248
           PPDGPE WP+ D+  QWLVFYRLD  T +G GT+EGNGQKWW+LPCKPHRGP+GS+  GP
Sbjct: 188 PPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGP 247

Query: 249 CDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIE 308
           C SP +IRFFMS+N+ V GLRIQNSPQFHMKFDGC+GVLI ++ ISSPKLSPNTDGIH+ 
Sbjct: 248 CASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLG 307

Query: 309 NTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNA 368
           NT+ VGIYNS++ NGDDCISIG GC++VDI+GVTCGPSHGI                   
Sbjct: 308 NTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGIS------------------ 367

Query: 369 VNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMEN 428
                 IGSLGVHNSQACVSNITVRN VIR+SDNG+R+KTWQGG+G+VS++LFENIQMEN
Sbjct: 368 ------IGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMEN 427

Query: 429 VRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITM 488
           V NCIIVDQYYC SKDCRN+TSAV V  V Y+NIKGTYDVR+ PIHFACSDTVACTNITM
Sbjct: 428 VLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITM 475

Query: 489 SEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE--YSC 537
           SEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G+P  V E Y+  Y C
Sbjct: 488 SEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPV-VEEAYDSNYGC 475

[Close]

BLAST of CmoCh08G012630 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 7.4e-79
Identity = 171/451 (37.92%), Postives = 238/451 (52.77%), Query Frame = 0


Query: 87  VFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLV 146
           +F+V  YGAVGDG  D T AF  AW+AAC   SA LLVP ++ F + +  F+GPC+P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 147 FKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLMPPDGPESWPKADSSRQWLVFYR 206
           FK                              VDG +     P SW    ++R WL F +
Sbjct: 118 FK------------------------------VDGIIAAYQNPASW---KNNRIWLQFAK 177

Query: 207 LDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRI 266
           L   TL G G I+G G++WW   CK     NG  +    D P  I+F  S+ L + GL++
Sbjct: 178 LTGFTLMGKGVIDGQGKQWWAGQCK---WVNGREICNDRDRPTAIKFDFSTGLIIQGLKL 237

Query: 267 QNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIG 326
            NSP+FH+ F  CEGV I  +SI++P+ SPNTDGI I  +K   +  + IG GDDC++IG
Sbjct: 238 MNSPEFHLVFGNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIG 297

Query: 327 PGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNI 386
            G +N+ IE + CGP HGI                         IGSLG  NS+A VS +
Sbjct: 298 TGSSNIVIEDLICGPGHGIS------------------------IGSLGRENSRAEVSYV 357

Query: 387 TVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKD-CRNQT 446
            V  A   ++ NG+RIKTWQGGSG  S I++EN++M N  N I+++Q+YC S   C+NQ 
Sbjct: 358 HVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQNQR 417

Query: 447 SAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCW-- 506
           SAV +  V YKNI+GT       I   CSD++ C +I +S++ L    G++     C   
Sbjct: 418 SAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKIAS---CLND 444

Query: 507 NAFGIQETLTIPPIDCLQEGAPQNVAETYEY 535
           NA G      IP    L   A +  ++++++
Sbjct: 478 NANGYFSGHVIPACKNLSPSAKRKESKSHKH 444

[Close]

BLAST of CmoCh08G012630 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.1e-77
Identity = 165/442 (37.33%), Postives = 227/442 (51.36%), Query Frame = 0


Query: 84  SDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESAT-LLVPSDRCFKITSTIFSGPCK 143
           S+ +  V N+GA G+G  DDT AF  AWK AC+ +  T +LVP +    +     SGPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 144 PGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLMPPDGPESWPKADSSRQWL 203
             L                              T Q+ GT++ P+ P+ W +  + R+WL
Sbjct: 109 ARL------------------------------TLQISGTIIAPNDPDVW-EGLNRRKWL 168

Query: 204 VFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPC-DSPALIRFFMSSNLAV 263
            F+ L ++T+ G GT+ G GQ+WWE  CK H   N      PC  +P  + F    N+ V
Sbjct: 169 YFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNHSN------PCRGAPTALTFHKCKNMRV 228

Query: 264 SGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDD 323
             L + +S Q H+    C  V I  L + +P  SPNTDGIHI  ++G+ I N+ +  GDD
Sbjct: 229 ENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDD 288

Query: 324 CISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQA 383
           CISI      + I  + CGP HGI                         IGSLG   S  
Sbjct: 289 CISIVKNSTQISISNIICGPGHGIS------------------------IGSLGKSKSWE 348

Query: 384 CVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLS-KD 443
            V +ITV  A+I ++ NGVRIKTWQGGSG VS I+F NI+M NV N II+DQYYC S K 
Sbjct: 349 EVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKP 408

Query: 444 CRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDP 503
           C NQTSA+ +  + + +++GT       I  +CSD+  C NI + +++L P  G+   + 
Sbjct: 409 CANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTES 426

Query: 504 FCWNAFGIQETLTIPPIDCLQE 523
           FCW A+G       PP  CL +
Sbjct: 469 FCWEAYGSSSGQVYPP-PCLSD 426

[Close]

BLAST of CmoCh08G012630 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.0e-76
Identity = 166/404 (41.09%), Postives = 216/404 (53.47%), Query Frame = 0


Query: 87  VFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLV 146
           VF+V  YGAVGDG  D T AF   W AAC   SA LLVP+++ F + + +F GPC+P L 
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117

Query: 147 FKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLMPPDGPESWPKADSSRQWLVFYR 206
           FK                              VDGT++    P  W    +S+ WL F +
Sbjct: 118 FK------------------------------VDGTIVAQPDPARW---KNSKIWLQFAQ 177

Query: 207 LDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRI 266
           L    L GTG I+G GQ+WW   CK     NG T+    + P  I+   S ++ V  L +
Sbjct: 178 LTDFNLMGTGVIDGQGQQWWAGQCKV---VNGRTVCNDRNRPTAIKIDYSKSVTVKELTL 237

Query: 267 QNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIG 326
            NSP+FH+ F  CEGV I+ L I +P+ SPNTDGI I  +K   I   +IG GDDCI+IG
Sbjct: 238 MNSPEFHLVFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIG 297

Query: 327 PGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNI 386
            G +N+ I+ + CGP HGI                         IGSLG  NS+A VS++
Sbjct: 298 TGSSNITIKDLICGPGHGIS------------------------IGSLGRDNSRAEVSHV 357

Query: 387 TVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKD-CRNQT 446
            V  A   ++ NG+RIKTWQGGSG  S I +EN++M N  N I+++Q+YC S   C+NQ 
Sbjct: 358 HVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQNQR 400

Query: 447 SAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVEL 490
           SAV +  V YKNI GT       I   CSD+V CT I +S V L
Sbjct: 418 SAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSL 400

[Close]

BLAST of CmoCh08G012630 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 3.2e-74
Identity = 167/440 (37.95%), Postives = 229/440 (52.05%), Query Frame = 0


Query: 89  DVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFK 148
           +V ++GA GDG  DDT AF  AWKAAC+  S+ +L+   + + +    FSGPCK GL   
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGL--- 150

Query: 149 VSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLMPPDGPESWPKADSSRQWLVFYRLD 208
                                      T Q+ GT+   D    + K    R WLVF  + 
Sbjct: 151 ---------------------------TMQIYGTIEASDDRSDYRK--DGRHWLVFDSVQ 210

Query: 209 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPC-DSPALIRFFMSSNLAVSGLRIQ 268
            + + G GTI GNG+ WW+  CK ++         PC D+P  + F+ S ++ V  L+I+
Sbjct: 211 NLRVEGGGTINGNGKIWWQNSCKTNKAL-------PCKDAPTALTFYKSKHVIVKNLKIE 270

Query: 269 NSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGP 328
           N+ Q H+ FD C  V    L +++P+ SPNTDGIH+  T+ + I + +IG GDDCISI  
Sbjct: 271 NAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVN 330

Query: 329 GCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNIT 388
           G   V +  +TCGP HGI                         IGSLG  NS+A VS++ 
Sbjct: 331 GSRKVRVNDITCGPGHGIS------------------------IGSLGYGNSEAHVSDVV 390

Query: 389 VRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYC-LSKDCRNQTS 448
           V  A +  + NGVRIKTWQGGSG+ S+I F+N++M NV N II+DQ YC   K C+ Q+S
Sbjct: 391 VNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSS 450

Query: 449 AVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAF 508
           AV V  V+Y+NIKGT    N  I F CS    C  I + +V+ L  EG       C N  
Sbjct: 451 AVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAKALC-NNV 462

Query: 509 GIQETLTIPPIDCLQEGAPQ 527
            + ET  + P  C +EG  +
Sbjct: 511 ELSETGVVSP-HCQEEGGEE 462

[Close]

BLAST of CmoCh08G012630 vs. ExPASy TrEMBL
Match: A0A6J1EWI8 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 PE=3 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.0e-280
Identity = 481/536 (89.74%), Postives = 481/536 (89.74%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVD 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFK                              VD
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFK------------------------------VD 180

Query: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240
           GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST
Sbjct: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240

Query: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300
           LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG
Sbjct: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300

Query: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLS 360
           IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGI               
Sbjct: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIS-------------- 360

Query: 361 LVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420
                     IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI
Sbjct: 361 ----------IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420

Query: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480
           QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT
Sbjct: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480

Query: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 537
           NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC
Sbjct: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 482

[Close]

BLAST of CmoCh08G012630 vs. ExPASy TrEMBL
Match: A0A6J1KH63 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 476/536 (88.81%), Postives = 478/536 (89.18%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDS P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPAD APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADQAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVD 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFK                              VD
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFK------------------------------VD 180

Query: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240
           GTLMPPDGPESWPKADS RQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST
Sbjct: 181 GTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240

Query: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300
           LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLSPNTDG
Sbjct: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDG 300

Query: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLS 360
           IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGI               
Sbjct: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIS-------------- 360

Query: 361 LVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420
                     IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI
Sbjct: 361 ----------IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420

Query: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480
           QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQ+LYKNIKGTYDVRNTPIHFACSDTVACT
Sbjct: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFACSDTVACT 480

Query: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 537
           NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC
Sbjct: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 482

[Close]

BLAST of CmoCh08G012630 vs. ExPASy TrEMBL
Match: A0A6J1CB48 (polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.1e-258
Identity = 445/544 (81.80%), Postives = 464/544 (85.29%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRV A+++ ITAIL+ EN SNVQGRYHFHKGQKKQ PDE PS+PSPVYSPPPE++VP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  ST--------PADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWK 120
           S+        PA+PAPSVPSDPYPDDP N+TSDCVFDVT+YGAVGDGC DDTAAFRAAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKP 180
           AACAVESAT+LVPSD CF ITSTIFSGPCKPGLVFK                        
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFK------------------------ 180

Query: 181 VNATNQVDGTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKP 240
                 VDG+LMPPDGPESWPKADS RQWLVFYRLDQMT+TGTGTIEGNGQKWWELPCKP
Sbjct: 181 ------VDGSLMPPDGPESWPKADSVRQWLVFYRLDQMTMTGTGTIEGNGQKWWELPCKP 240

Query: 241 HRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSP 300
           HRGPNGSTLPGPCDSPALIRFFMSSNL VSGLRIQNSPQFHMKFDGCEGVLIEKLSISSP
Sbjct: 241 HRGPNGSTLPGPCDSPALIRFFMSSNLVVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSP 300

Query: 301 KLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRL 360
           KLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPGCANVDIEGVTCGPSHGI       
Sbjct: 301 KLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVDIEGVTCGPSHGIS------ 360

Query: 361 QSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 420
                             IGSLGVHNSQACVSNITVRNAVIR+SDNGVRIKTWQGGSG+V
Sbjct: 361 ------------------IGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQGGSGSV 420

Query: 421 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 480
           SDILFENIQMENVRNCIIVDQYYCLSK C NQTSAVFVNQVLY+NIKGTYDVRNTPIHFA
Sbjct: 421 SDILFENIQMENVRNCIIVDQYYCLSKGCLNQTSAVFVNQVLYRNIKGTYDVRNTPIHFA 480

Query: 481 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 537
           CSDTVACTNITMSEVELLPHEGELVEDPFCWNA+GIQETLTIPPIDCLQ+G PQNVAET 
Sbjct: 481 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAYGIQETLTIPPIDCLQDGQPQNVAETS 490

[Close]

BLAST of CmoCh08G012630 vs. ExPASy TrEMBL
Match: A0A6J1CE60 (polygalacturonase At1g48100 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.8e-253
Identity = 445/571 (77.93%), Postives = 464/571 (81.26%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRV A+++ ITAIL+ EN SNVQGRYHFHKGQKKQ PDE PS+PSPVYSPPPE++VP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  ST--------PADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWK 120
           S+        PA+PAPSVPSDPYPDDP N+TSDCVFDVT+YGAVGDGC DDTAAFRAAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKP 180
           AACAVESAT+LVPSD CF ITSTIFSGPCKPGLVFK                        
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFK------------------------ 180

Query: 181 VNATNQVDGTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKP 240
                 VDG+LMPPDGPESWPKADS RQWLVFYRLDQMT+TGTGTIEGNGQKWWELPCKP
Sbjct: 181 ------VDGSLMPPDGPESWPKADSVRQWLVFYRLDQMTMTGTGTIEGNGQKWWELPCKP 240

Query: 241 HR---------------------------GPNGSTLPGPCDSPALIRFFMSSNLAVSGLR 300
           HR                           GPNGSTLPGPCDSPALIRFFMSSNL VSGLR
Sbjct: 241 HRVGYPHYIYPLHYHSFSFNQRLNYFSLQGPNGSTLPGPCDSPALIRFFMSSNLVVSGLR 300

Query: 301 IQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISI 360
           IQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMI NGDDCISI
Sbjct: 301 IQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDCISI 360

Query: 361 GPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQACVSN 420
           GPGCANVDIEGVTCGPSHGI                         IGSLGVHNSQACVSN
Sbjct: 361 GPGCANVDIEGVTCGPSHGIS------------------------IGSLGVHNSQACVSN 420

Query: 421 ITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQT 480
           ITVRNAVIR+SDNGVRIKTWQGGSG+VSDILFENIQMENVRNCIIVDQYYCLSK C NQT
Sbjct: 421 ITVRNAVIRDSDNGVRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSKGCLNQT 480

Query: 481 SAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNA 537
           SAVFVNQVLY+NIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNA
Sbjct: 481 SAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNA 517

[Close]

BLAST of CmoCh08G012630 vs. ExPASy TrEMBL
Match: A0A6J1L0G9 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111499252 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 2.0e-244
Identity = 427/550 (77.64%), Postives = 452/550 (82.18%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQP-SLPSPVYSPP----- 60
           MEFN    VM WITAI++ +N SNV GRYHFHKGQ+ Q P E P S   P YSPP     
Sbjct: 1   MEFNGALIVMAWITAIVLVQNCSNVHGRYHFHKGQRTQKPGEDPCSDAPPPYSPPEEAVP 60

Query: 61  --PEDSVPST------PADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAA 120
             P +S PST      PA+PAPSVP+DPYPDDPGN+T+DCVFDVT++GAVGDGCADDTAA
Sbjct: 61  SSPAESAPSTPAESAPPAEPAPSVPADPYPDDPGNSTADCVFDVTDFGAVGDGCADDTAA 120

Query: 121 FRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVG 180
           FRAAWKAACAVESATLLVPSDRCFKITSTIF+GPC+PGLVFK                  
Sbjct: 121 FRAAWKAACAVESATLLVPSDRCFKITSTIFTGPCEPGLVFK------------------ 180

Query: 181 TLISKPVNATNQVDGTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWW 240
                       VDGTLMPPDGPESW K DS RQWLVFYRLDQMTLTG+GTIEGNGQKWW
Sbjct: 181 ------------VDGTLMPPDGPESWLKTDSPRQWLVFYRLDQMTLTGSGTIEGNGQKWW 240

Query: 241 ELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEK 300
           ELPCKPHRGPNGSTLPGPCDSPALIRFFMS+NLAVSGLRIQNSPQFH+KFDGCE VLI+K
Sbjct: 241 ELPCKPHRGPNGSTLPGPCDSPALIRFFMSTNLAVSGLRIQNSPQFHIKFDGCEDVLIQK 300

Query: 301 LSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIR 360
           LSISSPKLSPNTDGIHI++TKGVGIYNS+I NGDDCISIGPGC NVDIEGVTCGP HGI 
Sbjct: 301 LSISSPKLSPNTDGIHIQDTKGVGIYNSIISNGDDCISIGPGCTNVDIEGVTCGPGHGIS 360

Query: 361 YKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQ 420
                                   IGSLGVHNS+ACVSNITVRNAVIRESDNGVRIKTWQ
Sbjct: 361 ------------------------IGSLGVHNSKACVSNITVRNAVIRESDNGVRIKTWQ 420

Query: 421 GGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRN 480
           GGSG+VSDI FENIQMENVRNCIIVDQYYCL+KDC NQTSAVFVNQVLYKNIKGTYDVRN
Sbjct: 421 GGSGSVSDISFENIQMENVRNCIIVDQYYCLTKDCLNQTSAVFVNQVLYKNIKGTYDVRN 480

Query: 481 TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQ 537
           TP+HFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQ+TLTIPPIDCLQEG PQ
Sbjct: 481 TPVHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQKTLTIPPIDCLQEGEPQ 496

[Close]

BLAST of CmoCh08G012630 vs. NCBI nr
Match: XP_022930280.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 973.8 bits (2516), Expect = 6.1e-280
Identity = 481/536 (89.74%), Postives = 481/536 (89.74%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVD 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFK                              VD
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFK------------------------------VD 180

Query: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240
           GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST
Sbjct: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240

Query: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300
           LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG
Sbjct: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300

Query: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLS 360
           IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGI               
Sbjct: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIS-------------- 360

Query: 361 LVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420
                     IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI
Sbjct: 361 ----------IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420

Query: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480
           QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT
Sbjct: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480

Query: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 537
           NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC
Sbjct: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 482

[Close]

BLAST of CmoCh08G012630 vs. NCBI nr
Match: KAG7024752.1 (Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 968.0 bits (2501), Expect = 3.4e-278
Identity = 478/536 (89.18%), Postives = 479/536 (89.37%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPADPAP VPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPPVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVD 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFK                              VD
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFK------------------------------VD 180

Query: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240
           GTLMPPDGPESWPKADS RQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST
Sbjct: 181 GTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240

Query: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300
           LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLSPNTDG
Sbjct: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDG 300

Query: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLS 360
           IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGI               
Sbjct: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIS-------------- 360

Query: 361 LVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420
                     IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI
Sbjct: 361 ---------SIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420

Query: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480
           QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT
Sbjct: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480

Query: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 537
           NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC
Sbjct: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 483

[Close]

BLAST of CmoCh08G012630 vs. NCBI nr
Match: XP_023000015.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 965.3 bits (2494), Expect = 2.2e-277
Identity = 476/536 (88.81%), Postives = 478/536 (89.18%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDS P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPAD APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADQAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVD 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFK                              VD
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFK------------------------------VD 180

Query: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240
           GTLMPPDGPESWPKADS RQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST
Sbjct: 181 GTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240

Query: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300
           LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLSPNTDG
Sbjct: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDG 300

Query: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLS 360
           IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGI               
Sbjct: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIS-------------- 360

Query: 361 LVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420
                     IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI
Sbjct: 361 ----------IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420

Query: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480
           QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQ+LYKNIKGTYDVRNTPIHFACSDTVACT
Sbjct: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFACSDTVACT 480

Query: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 537
           NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC
Sbjct: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 482

[Close]

BLAST of CmoCh08G012630 vs. NCBI nr
Match: XP_023514293.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 963.8 bits (2490), Expect = 6.3e-277
Identity = 475/536 (88.62%), Postives = 478/536 (89.18%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DSVP
Sbjct: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60

Query: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVD 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFK                              VD
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFK------------------------------VD 180

Query: 181 GTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240
           GTLMPPDGPESWPKADS RQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST
Sbjct: 181 GTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGST 240

Query: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDG 300
           LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLSPNTDG
Sbjct: 241 LPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDG 300

Query: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLS 360
           IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGI               
Sbjct: 301 IHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIS-------------- 360

Query: 361 LVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420
                     IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI
Sbjct: 361 ----------IGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENI 420

Query: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480
           QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT
Sbjct: 421 QMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACT 480

Query: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 537
           NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC
Sbjct: 481 NITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 482

[Close]

BLAST of CmoCh08G012630 vs. NCBI nr
Match: XP_022138804.1 (polygalacturonase At1g48100 isoform X2 [Momordica charantia])

HSP 1 Score: 899.4 bits (2323), Expect = 1.5e-257
Identity = 445/544 (81.80%), Postives = 464/544 (85.29%), Query Frame = 0


Query: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60
           MEFNRV A+++ ITAIL+ EN SNVQGRYHFHKGQKKQ PDE PS+PSPVYSPPPE++VP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  ST--------PADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWK 120
           S+        PA+PAPSVPSDPYPDDP N+TSDCVFDVT+YGAVGDGC DDTAAFRAAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKP 180
           AACAVESAT+LVPSD CF ITSTIFSGPCKPGLVFK                        
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFK------------------------ 180

Query: 181 VNATNQVDGTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKP 240
                 VDG+LMPPDGPESWPKADS RQWLVFYRLDQMT+TGTGTIEGNGQKWWELPCKP
Sbjct: 181 ------VDGSLMPPDGPESWPKADSVRQWLVFYRLDQMTMTGTGTIEGNGQKWWELPCKP 240

Query: 241 HRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSP 300
           HRGPNGSTLPGPCDSPALIRFFMSSNL VSGLRIQNSPQFHMKFDGCEGVLIEKLSISSP
Sbjct: 241 HRGPNGSTLPGPCDSPALIRFFMSSNLVVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSP 300

Query: 301 KLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRL 360
           KLSPNTDGIHIENTKGVGIYNSMI NGDDCISIGPGCANVDIEGVTCGPSHGI       
Sbjct: 301 KLSPNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVDIEGVTCGPSHGIS------ 360

Query: 361 QSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 420
                             IGSLGVHNSQACVSNITVRNAVIR+SDNGVRIKTWQGGSG+V
Sbjct: 361 ------------------IGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQGGSGSV 420

Query: 421 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 480
           SDILFENIQMENVRNCIIVDQYYCLSK C NQTSAVFVNQVLY+NIKGTYDVRNTPIHFA
Sbjct: 421 SDILFENIQMENVRNCIIVDQYYCLSKGCLNQTSAVFVNQVLYRNIKGTYDVRNTPIHFA 480

Query: 481 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETY 537
           CSDTVACTNITMSEVELLPHEGELVEDPFCWNA+GIQETLTIPPIDCLQ+G PQNVAET 
Sbjct: 481 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAYGIQETLTIPPIDCLQDGQPQNVAETS 490

[Close]

BLAST of CmoCh08G012630 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 667.5 bits (1721), Expect = 8.7e-192
Identity = 335/534 (62.73%), Postives = 392/534 (73.41%), Query Frame = 0


Query: 9   VMLWITAILVAENLSNV-QGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADPA 68
           ++++I + ++  + +NV + RYH+HKG+          +  P+  PPP    P   A+P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH--------GVTHPLPPPPPP---PLETANPP 67

Query: 69  PSVPSDPYPDD---PGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLV 128
             VPSDPYP     PG++ S CVFDVT++GAVGDG  DDTAAF+ AWKAACAVES  +L 
Sbjct: 68  DQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLA 127

Query: 129 PSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLM 188
           P    FKITSTIFSGPCKPGLVF                              Q+DG LM
Sbjct: 128 PEGGVFKITSTIFSGPCKPGLVF------------------------------QLDGVLM 187

Query: 189 PPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGP 248
           PPDGPE WP+ D+  QWLVFYRLD  T +G GT+EGNGQKWW+LPCKPHRGP+GS+  GP
Sbjct: 188 PPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGP 247

Query: 249 CDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIE 308
           C SP +IRFFMS+N+ V GLRIQNSPQFHMKFDGC+GVLI ++ ISSPKLSPNTDGIH+ 
Sbjct: 248 CASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLG 307

Query: 309 NTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNA 368
           NT+ VGIYNS++ NGDDCISIG GC++VDI+GVTCGPSHGI                   
Sbjct: 308 NTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGIS------------------ 367

Query: 369 VNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMEN 428
                 IGSLGVHNSQACVSNITVRN VIR+SDNG+R+KTWQGG+G+VS++LFENIQMEN
Sbjct: 368 ------IGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMEN 427

Query: 429 VRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITM 488
           V NCIIVDQYYC SKDCRN+TSAV V  V Y+NIKGTYDVR+ PIHFACSDTVACTNITM
Sbjct: 428 VLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITM 475

Query: 489 SEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE--YSC 537
           SEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G+P  V E Y+  Y C
Sbjct: 488 SEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPV-VEEAYDSNYGC 475

[Close]

BLAST of CmoCh08G012630 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 525.4 bits (1352), Expect = 5.4e-149
Identity = 275/535 (51.40%), Postives = 346/535 (64.67%), Query Frame = 0


Query: 9   VMLWITAILVAENLSNVQGRYH--FHKGQKKQNPDEQPSLPSPVYSPPPEDSVPSTPADP 68
           V+ ++T IL   +LS  +  +H   HK     +   +P  PS   S PP    P  P  P
Sbjct: 11  VLGFMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPP-PGPPDSP 70

Query: 69  APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESAT---LL 128
           APS+P  P  DDP +  +  +++V  YGAVGDG  DDT AF+ AW ++C  E+ T   LL
Sbjct: 71  APSLPPSP-SDDPADDNNG-IYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLL 130

Query: 129 VPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTL 188
           VP    F I STIF+GPC+    F                              QVDGT+
Sbjct: 131 VPYGYTFMIQSTIFTGPCRSYQFF------------------------------QVDGTI 190

Query: 189 MPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTL-P 248
           + PDGPESWP   S RQWLVFYR++ M L G G I+G GQKWW+LPCKPHR  N S +  
Sbjct: 191 VTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVIDGRGQKWWDLPCKPHRSVNKSAIVT 250

Query: 249 GPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIH 308
           GPCDSP  +RFFMSSNL V GL+I+NSPQFH +FDGC+GV +E L I++P LSPNTDGIH
Sbjct: 251 GPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDGCQGVHVESLHITAPPLSPNTDGIH 310

Query: 309 IENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGP-SHGIRYKQRRLQSPFICLSL 368
           IEN+  V IYNS+I NGDDC+SIG G  +VDI  +TCGP  HGI                
Sbjct: 311 IENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLTCGPGGHGIS--------------- 370

Query: 369 VNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQ 428
                    IGSLG HNS+ACVSNITVR++VI+ SDNGVRIKTWQGGSG+VS + F NI 
Sbjct: 371 ---------IGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIH 430

Query: 429 MENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTN 488
           +++VRN II+DQYYC++KDC N+TSAVFV+ + Y+ IKGTYD+R+ P+HF CSD V CTN
Sbjct: 431 VDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTN 488

Query: 489 ITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 537
           +T+S++ELLP +GE+V DPFCWNA+GI E L+IPP+ CL    P+ +  +    C
Sbjct: 491 LTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGLQGSLVDKC 488

[Close]

BLAST of CmoCh08G012630 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 482.3 bits (1240), Expect = 5.2e-136
Identity = 239/463 (51.62%), Postives = 306/463 (66.09%), Query Frame = 0


Query: 66  PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAAC---AVESATL 125
           P+P+ P+  Y D+  +  +  VFDVT++GA+GD   DDT+AF+ AW AAC     +SA L
Sbjct: 29  PSPA-PNPAYNDN--DNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALL 88

Query: 126 LVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGT 185
           LVP   CF +  T F+GPC+  LV                               Q+DG 
Sbjct: 89  LVPYTFCFLVKPTTFNGPCRTNLVL------------------------------QIDGF 148

Query: 186 LMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLP 245
           ++ PDGP SWP ++  RQW++FYR++ +++ G+G I G GQKWW LPCKPH+G NG+T  
Sbjct: 149 IVSPDGPRSWP-SNYQRQWMMFYRVNGLSIQGSGVINGRGQKWWNLPCKPHKGLNGTTQT 208

Query: 246 GPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIH 305
           GPCDSP  IR F SS + + G+   NS QFH++FD C  V+++ + I +P  SPNTDGIH
Sbjct: 209 GPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVRFDNCSDVVVDSVIIKAPASSPNTDGIH 268

Query: 306 IENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLV 365
           IENT  V I NSMI NGDDCISIG GC NVDI+ VTCGPSHGI                 
Sbjct: 269 IENTHNVQIRNSMISNGDDCISIGAGCFNVDIKNVTCGPSHGIS---------------- 328

Query: 366 NAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQM 425
                   IGSLGVHNSQA VSNITV N+ I  SDNGVRIKTWQGGSG+VS I+F NI M
Sbjct: 329 --------IGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFSNILM 388

Query: 426 ENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNI 485
            NVRN I++DQYYC + +C NQTSAV ++ VLY NIKGTYD+R+ PIHF CSD+V CTN+
Sbjct: 389 VNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSVPCTNL 432

Query: 486 TMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAP 526
           T++EV+L P +G+ +E+PFCWNA+G  + +T+PP+ CL +  P
Sbjct: 449 TLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

[Close]

BLAST of CmoCh08G012630 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 467.6 bits (1202), Expect = 1.3e-131
Identity = 235/469 (50.11%), Postives = 304/469 (64.82%), Query Frame = 0


Query: 61  STPADPAPSVPSDP--YPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120
           S P  P+P+ P+DP   P    +   + V+DV  YGAVG+G ADDT +F+ AW +AC+  
Sbjct: 37  SQPPSPSPN-PNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACSNN 96

Query: 121 ----SATLLVPSDRCFKITSTIFSGPCKPGLVFKVSHIFLEFESLVVRVEVGTLISKPVN 180
               ++ L VP    F I STIF+GPC+    F                           
Sbjct: 97  KNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYF--------------------------- 156

Query: 181 ATNQVDGTLMPPDGPESWPKADSSRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPH- 240
              QVDGT++P DGP+SWP + + RQWL FYR++ M L G G I+G GQ WW+LPCKPH 
Sbjct: 157 ---QVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQ 216

Query: 241 RGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPK 300
           +  N + L GPC+SPA +RFFMSSN+ V GL I+NSPQ H+K DGC  V I  L I SP 
Sbjct: 217 QNVNKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPP 276

Query: 301 LSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCANVDIEGVTCGP-SHGIRYKQRRL 360
            SPNTDGIHIEN+  V IYNS+I NGDDC+SIGPG  ++DI  +TCGP  HGI       
Sbjct: 277 ASPNTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGIS------ 336

Query: 361 QSPFICLSLVNAVNNSLFIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTV 420
                             IGSLG  NS ACVSN+TVR++ I+ S+NGVRIKTWQGGSG+V
Sbjct: 337 ------------------IGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQGGSGSV 396

Query: 421 SDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFA 480
           S + F+NI ++ VRN II+DQYYC +K C N+TSAVFVN ++Y++IKGTYD+R+ P+HF 
Sbjct: 397 SGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFG 450

Query: 481 CSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQ 522
           CS+ V CTN+T+S +ELLP + ++V  PFCWNA+GI +  ++P I CL+
Sbjct: 457 CSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLK 450

[Close]

BLAST of CmoCh08G012630 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 342.0 bits (876), Expect = 8.4e-94
Identity = 186/456 (40.79%), Postives = 252/456 (55.26%), Query Frame = 0


Query: 84  SDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSG-PCK 143
           S    +V ++GA GDG +DDT AF  AW+ AC V ++TLLVPS   F +    F G  CK
Sbjct: 47  SSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECK 106

Query: 144 PGLVFKVSHIFLEFESLVVRVEVGTLISKPVNATNQVDGTLMPPDGPESWPKADSSRQWL 203
             +VF                              Q++G ++ P    +W       QW+
Sbjct: 107 EKIVF------------------------------QLEGKIIAPTSASAW--GSGLLQWI 166

Query: 204 VFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVS 263
            F  L  +T+ G G I+G G  WW           G+ +P     P  +RF+ S+ + VS
Sbjct: 167 EFKALQGITIKGKGIIDGRGSVWW-------NDMMGTKMPR--TKPTALRFYGSNGVTVS 226

Query: 264 GLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDC 323
           G+ IQNSPQ H+KFD C  + +   + SSP  SPNTDGIH++N++   IY S +  GDDC
Sbjct: 227 GITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDC 286

Query: 324 ISIGPGCANVDIEGVTCGPSHGIRYKQRRLQSPFICLSLVNAVNNSLFIGSLGVHNSQAC 383
           ISI  GC+N++I  V CGP HGI                         IG LG  N++AC
Sbjct: 287 ISIQTGCSNINIHDVDCGPGHGIS------------------------IGGLGKDNTKAC 346

Query: 384 VSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCR 443
           VSNITVR+  + E+ NGVRIK+WQGGSG+V  ++F NIQ+ NV N II+DQYYC    C 
Sbjct: 347 VSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGGCH 406

Query: 444 NQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVE-DPF 503
           N+TSAV V+ + Y NIKGTY     P+ FACSD++ CT I++S +EL P  G+    DPF
Sbjct: 407 NETSAVAVSNINYINIKGTY--TKEPVRFACSDSLPCTGISLSTIELKPATGKASSLDPF 435

Query: 504 CWNAFGIQETLTIPPIDCLQ-EGAPQNVAETYEYSC 537
           CW A G  +T T+PPI CL+ E +P+  + +   +C
Sbjct: 467 CWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

The following BLAST results are available for this feature:

BLAST of CmoCh08G012630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	1.2e-190	62.73	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
P43212	7.4e-79	37.92	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q94AJ5	9.1e-77	37.33	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q7M1E7	2.0e-76	41.09	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P35336	3.2e-74	37.95	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]

BLAST of CmoCh08G012630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EWI8	3.0e-280	89.74	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 P...	[more]
A0A6J1KH63	1.1e-277	88.81	polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=...	[more]
A0A6J1CB48	7.1e-258	81.80	polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CE60	1.8e-253	77.93	polygalacturonase At1g48100 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1L0G9	2.0e-244	77.64	polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111499252 PE=...	[more]

BLAST of CmoCh08G012630 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022930280.1	6.1e-280	89.74	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
KAG7024752.1	3.4e-278	89.18	Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023000015.1	2.2e-277	88.81	polygalacturonase At1g48100-like [Cucurbita maxima]	[more]
XP_023514293.1	6.3e-277	88.62	polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo]	[more]
XP_022138804.1	1.5e-257	81.80	polygalacturonase At1g48100 isoform X2 [Momordica charantia]	[more]

BLAST of CmoCh08G012630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48100.1	8.7e-192	62.73	Pectin lyase-like superfamily protein	[more]
AT1G02460.1	5.4e-149	51.40	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	5.2e-136	51.62	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	1.3e-131	50.11	Pectin lyase-like superfamily protein	[more]
AT5G14650.1	8.4e-94	40.79	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 329..349 e-value: 8600.0 score: 0.5 coord: 306..327 e-value: 6400.0 score: 1.5 coord: 412..433 e-value: 390.0 score: 9.8 coord: 279..305 e-value: 520.0 score: 9.2 coord: 383..404 e-value: 11.0 score: 15.0
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 370..489 e-value: 3.1E-34 score: 118.5 coord: 121..345 e-value: 2.7E-38 score: 131.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 80..533 e-value: 2.7E-111 score: 374.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..81
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..75
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 367..527
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 8..151
None	No IPR available	PANTHER	PTHR31375:SF142	POLYGALACTURONASE PLANT-LIKE PROTEIN	coord: 367..527 coord: 176..362
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 176..362
None	No IPR available	PANTHER	PTHR31375:SF142	POLYGALACTURONASE PLANT-LIKE PROTEIN	coord: 8..151
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 86..529

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G012630.1	CmoCh08G012630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity