CmoCh08G005640 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh08G005640 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.2e-229
Identity = 388/534 (72.66%), Postives = 457/534 (85.58%), Query Frame = 0

Query: 10  EFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQLTARRVASG 69
           + IFRSKLPDI IPNHLPLH Y F+N+  ++SRPCLINGA   +Y+Y DV+L +R+VA+G
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAADDVVALPY 189
           VT AC  ++VKD A EN VKI+C+D A EGCLH+SVL+ A+E  +P V+   DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY    DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMSSVRVLKSG 309
           LR GAAILIMQKF+IVS LEL+++YK +I P VPPI+LAIAKS   + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 369
            APLGK+LEDTVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDDD 429
           MKI+DP+TG SLP N +GEICIRGDQIMKGYLND E+T RTIDKEGWL+TGDIG+IDDDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAFVVKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DEQAGE+PVAFVV++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGVYN 543
             ITE++VK FIS+QV+FYKR+KRVFFV+AIPK+PSGKILRK+LR++LAAG+ N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of CmoCh08G005640 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 5.7e-228
Identity = 384/543 (70.72%), Postives = 456/543 (83.98%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           M  E NQ  + IFRSKLPDI IP HLPLH Y F+N+  ++SRPCLINGA  H+Y+Y DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA+GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFA 180
           AKA+NAKLI+T ACF ++VKD A +N + ++C+D A EGC+H+S L+ ADE  +P V   
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIY 240
           +DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY    DV++CVLP FHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDM 300
           SLNS+LLCGLR GAAILIMQKF+IV   EL+EKYK +I P VPPI+LAIAKS   + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGK+LED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKI+DP+TG SLP N  GEICIRGDQIMKGYLND  +T RTIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELP 480
           DIG+ID+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DEQAGE+P
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAG 540
           VAFVV++NG +ITE++VK F+S+QV+FYKR+KRVFFV  +PK+PSGKILRK+LR++LAAG
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 VYN 543
           V N
Sbjct: 543 VPN 544

BLAST of CmoCh08G005640 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.1e-226
Identity = 383/540 (70.93%), Postives = 451/540 (83.52%), Query Frame = 0

Query: 4   EINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQLTA 63
           E  Q+ + IFRSKLPDI IP HLPLH Y F+N+  ++SRPCLINGA   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAADD 183
           ++AK+I+T +CF  +VKD A EN VK++C+D A EGCLH+S L+ +DE  +P V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY    DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMSSV 303
           SILLCGLR GAAILIMQKF+I   LEL++KYK SI P VPPI+LAIAKS   + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGT 363
           R + SG APLGK+LED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKI+DP+TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAF 483
           FID+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M DEQAGE+PVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGVYN 543
           VV++NG  ITE++VK FIS+QV+FYKR+KRVFFV  +PK+PSGKILRK+LR++LAAGV N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of CmoCh08G005640 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 2.4e-226
Identity = 390/547 (71.30%), Postives = 457/547 (83.55%), Query Frame = 0

Query: 1   MAAEI---NQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYD 60
           MAA +    Q K+ IFRSKLPDI IP +LPLH Y F+N+  ++SRPCLINGAT  +++Y 
Sbjct: 1   MAAAVAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYA 60

Query: 61  DVQLTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEI 120
           DV+L +RRV SGL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+
Sbjct: 61  DVELISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEV 120

Query: 121 AKQAKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYA-----VEGCLHYSVLSGADES 180
            KQAKA+NAKLI+T  C+ D+VKD A ENGVKI+ +D           LH+S L+GADE+
Sbjct: 121 IKQAKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADEN 180

Query: 181 HLPFVDFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILC 240
            +P V+ + D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   +DV+LC
Sbjct: 181 EMPKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLC 240

Query: 241 VLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKS 300
           VLP FHIYSLNS+LLCGLRAG+ ILIMQKFEIV  LEL++KYK +I P VPPI+LAIAKS
Sbjct: 241 VLPLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKS 300

Query: 301 SEFEKYDMSSVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKE 360
           +  + YD+SSVR + SG APLGK+LED VR KFP A LGQGYGMTEAGPVL M LAFAKE
Sbjct: 301 TVVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKE 360

Query: 361 PFPVKAGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTID 420
           PF +K+GACGTVVRNAEMKI+DPETG SLP N  GEICIRGDQIMKGYLND E+T RTID
Sbjct: 361 PFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTID 420

Query: 421 KEGWLHTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMP 480
           KEGWLHTGDIG+IDDDDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M 
Sbjct: 421 KEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMK 480

Query: 481 DEQAGELPVAFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKE 539
           DE AGE+PVAFVVK+NG  ITE+++KQFIS+QV+FYKR+ RVFFV AIPKAPSGKILRK+
Sbjct: 481 DEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKD 540

BLAST of CmoCh08G005640 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 3.2e-223
Identity = 379/543 (69.80%), Postives = 448/543 (82.50%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           M  E  Q+ + IFRSKLPDI IP HLPLH Y F+NL  + SRPCLI+GA   +Y+Y +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFA 180
           AKA++AK+++T ACF  +VKD A EN +K++CVD A EGC+H+S L  +DE  +P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDM 300
           SLNS+LLC LR GAAILIMQKF+I   LEL+ K+K +I P VPPI+LAIAKS     YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGA 360
           SSVR + SG APLGK+LED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKI+DP+TG SLP N  GEICIRGDQIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELP 480
           DIGFIDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M DEQAGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAG 540
           VAFVV++NG  ITE++VK FIS+QV+FYKR+KRVFFV  +PK+PSGKILRK+LR++LAAG
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 VYN 543
           + N
Sbjct: 543 ISN 545

BLAST of CmoCh08G005640 vs. ExPASy TrEMBL
Match: A0A6J1H6B4 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460853 PE=4 SV=1)

HSP 1 Score: 1072.8 bits (2773), Expect = 4.0e-310
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV
Sbjct: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmoCh08G005640 vs. ExPASy TrEMBL
Match: A0A6J1KVH9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497965 PE=4 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 5.4e-306
Identity = 534/542 (98.52%), Postives = 536/542 (98.89%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAEINQTKEFIFRSKLPDI IPNHLPLHEYIFQNLPNY SRPCLINGATGHVYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESH+PFVDF A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASI+PIVPPILLAIAKSSEFEKYDMS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKKLED VREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVK GAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVV ANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV
Sbjct: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmoCh08G005640 vs. ExPASy TrEMBL
Match: A0A6J1H7E7 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460855 PE=4 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 3.3e-287
Identity = 499/542 (92.07%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAE+NQT EFIFRSKLPDI IPNHLPLH Y+FQN   + SRPCLINGATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDYAVE CLHYSVLSGADESHLPFVDFAA
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG+NPNLYY   DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVS L+LVEKYK SIMPIVPPI LAIAKS + EKYD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGK+LED VREKFP AILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+DPETG SLPANSAGEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFID+DDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPDEQAGE+PV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGG ITEEDVKQFIS+QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA G 
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of CmoCh08G005640 vs. ExPASy TrEMBL
Match: A0A6J1KT15 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497966 PE=4 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 4.8e-286
Identity = 496/542 (91.51%), Postives = 513/542 (94.65%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAE+NQT EFIFRSKLPDI IPNHLPLH Y+FQN     SRPCLINGATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESH+PFVDFAA
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL+LVEKYK SIMPIVPPI LAIAKS + EKYD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGK+LED VREKFP AILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+DPETG SLPANSAGEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGF+DD++ELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPDE+AGE+PV
Sbjct: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGG ITEEDV QFIS QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++L  G 
Sbjct: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmoCh08G005640 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 9.0e-285
Identity = 492/542 (90.77%), Postives = 517/542 (95.39%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MA E NQT EFIFRSKLPDI IPNHLPLHEY+FQN+  + SRPCLINGATG +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           L +RRVA+GLHNLGIKKGDVVMNLLPNSPEFVFTFLGASY GA+MTAANPFYTAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVD+AVEGCLHYSVLSGADESH+P V+FAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLEL+EK+K SIMPIVPPI LAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGK+LED VREKFPTA+LGQGYGMTEAGPVLTMSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+DPETG SLPANSAGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFK FQVAPAELE+LLI HPKLSDAAVIGMPDEQAGE+PV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGG ITEEDVKQFIS+QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of CmoCh08G005640 vs. NCBI nr
Match: XP_022959956.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 1072.8 bits (2773), Expect = 8.3e-310
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV
Sbjct: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmoCh08G005640 vs. NCBI nr
Match: KAG6593347.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.5e-307
Identity = 539/542 (99.45%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAEINQTKE IFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATG VYSY DVQ
Sbjct: 1   MAAEINQTKELIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGDVYSYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV
Sbjct: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmoCh08G005640 vs. NCBI nr
Match: XP_023004760.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 1059.3 bits (2738), Expect = 1.1e-305
Identity = 534/542 (98.52%), Postives = 536/542 (98.89%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAEINQTKEFIFRSKLPDI IPNHLPLHEYIFQNLPNY SRPCLINGATGHVYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESH+PFVDF A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASI+PIVPPILLAIAKSSEFEKYDMS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKKLED VREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVK GAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVV ANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV
Sbjct: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmoCh08G005640 vs. NCBI nr
Match: XP_023515269.1 (4-coumarate--CoA ligase 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023515270.1 4-coumarate--CoA ligase 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1058.9 bits (2737), Expect = 1.5e-305
Identity = 535/542 (98.71%), Postives = 536/542 (98.89%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAEINQTKEFIFRSKLPDI IPNHL LHEYIFQNLPNY SRPCLINGATG VYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIHIPNHLTLHEYIFQNLPNYGSRPCLINGATGDVYSYDDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAANAKLIVTMACFYDRVKDL ENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEI+SLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIISLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGGEITEEDVK FISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV
Sbjct: 481 AFVVKANGGEITEEDVKHFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmoCh08G005640 vs. NCBI nr
Match: KAG6593348.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1000.0 bits (2584), Expect = 8.1e-288
Identity = 501/542 (92.44%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 1   MAAEINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQ 60
           MAAE+NQT EFIFRSKLPDI IPNHLPLH Y+FQN   + SRPCLINGATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVDYAVE CLHYSVLSGADESHLPFVDFAA
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG+NPNLYY   DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMS 300
           LNSILLCGLRAGAAILIMQKFEIVS L+LVEKYK SIMPIVPPI LAIAKS E EKYD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPELEKYDVS 300

Query: 301 SVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGK+LED VREKFP AILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKI+DPETG SLPANSAGEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480
           IGFIDDDDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIGMPDEQAGE+PV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540
           AFVVKANGG ITEEDVKQFIS+QVVFYKRLKRVFFVNAIPKAPSGKILRKELR++LA G 
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 YN 543
           Y+
Sbjct: 541 YD 542

BLAST of CmoCh08G005640 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 748.0 bits (1930), Expect = 5.1e-216
Identity = 370/544 (68.01%), Postives = 445/544 (81.80%), Query Frame = 0

Query: 6   NQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQLTARR 65
           N   + IFRSKLPDI IPNHL LH+YIFQN+  +A++PCLING TGHVY+Y DV + +R+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAAN 125
           +A+  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDRVKDLA-ENGVKIVCVD-----YAVEGCLHYSVLSGAD---ESHLPFV 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL ++ L+ +       +  V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 DFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFF 245
           + + DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  +DVILCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LLEL+++ K ++ P+VPPI+LAIAKSSE EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDMSSVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+RV+KSG APLGK+LED V  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWL 425
           +GACGTVVRNAEMKI+DP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAG 485
           HTGDIG IDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 ELPVAFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQL 541
           E+PVAFVVK+   E++E+DVKQF+S+QVVFYKR+ +VFF  +IPKAPSGKILRK+LR++L
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of CmoCh08G005640 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 743.8 bits (1919), Expect = 9.7e-215
Identity = 362/539 (67.16%), Postives = 449/539 (83.30%), Query Frame = 0

Query: 8   TKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQLTARRVA 67
           + + IFRS+LPDI IPNHLPLH+YIF+N+  +A++PCLING TG VY+Y DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  SGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           +GLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVKIVCVDYAV--EGCLHYSVLSGADESHLPFV--DFAADDV 187
           LIVT + + D++K+L  +GV IV  D     E CL +S L+ ++E  +  +    + +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+ R+DVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMSSVR 307
           I+LC LR GA ILIM KFEI  LLE +++ K ++  +VPPI+LAIAKS E EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTV 367
           ++KSG APLGK+LED +  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAFV 487
           IDDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M +E AGE+PVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGVYN 543
           V++    I+E+++KQF+S+QVVFYKR+ +VFF ++IPKAPSGKILRK+LR++LA G+ N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of CmoCh08G005640 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 696.4 bits (1796), Expect = 1.8e-200
Identity = 345/508 (67.91%), Postives = 413/508 (81.30%), Query Frame = 0

Query: 6   NQTKEFIFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQLTARR 65
           N   + IFRSKLPDI IPNHL LH+YIFQN+  +A++PCLING TGHVY+Y DV + +R+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAAN 125
           +A+  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDRVKDLA-ENGVKIVCVD-----YAVEGCLHYSVLSGAD---ESHLPFV 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL ++ L+ +       +  V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 DFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFF 245
           + + DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  +DVILCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LLEL+++ K ++ P+VPPI+LAIAKSSE EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDMSSVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+RV+KSG APLGK+LED V  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWL 425
           +GACGTVVRNAEMKI+DP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAG 485
           HTGDIG IDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 ELPVAFVVKANGGEITEEDVKQFISEQV 505
           E+PVAFVVK+   E++E+DVKQF+S+QV
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of CmoCh08G005640 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 678.7 bits (1750), Expect = 3.8e-195
Identity = 327/546 (59.89%), Postives = 426/546 (78.02%), Query Frame = 0

Query: 4   EINQTKEFIFRSKLPDIRIPNHLPLHEYIFQNL----PNYASRPCLINGATGHVYSYDDV 63
           E   + +FIFRSKLPDI IPNHLPL +Y+FQ         +S  C+I+GATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+A+G+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLAENGVKIVCVD---------YAVEGCLHYSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          + +GC+ ++ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHLPFVDFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVIL 243
           + L     + +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +  NDVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAK 303
           C LP FHIY+L++++L  +R GAA+LI+ +FE+  ++EL+++YK +++P+ PP++LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SSEFEKYDMSSVRVLKSGGAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAK 363
           S E E+YD+SSVR++ SG A L K+LED VR KFP AI GQGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFPVKAGACGTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTI 423
            PF  K+GACGTV+RNAEMK++D ETG+SLP N +GEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGM 483
           DK+GWLHTGDIGF+DDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDEQAGELPVAFVVKANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRK 537
            DE A E+PVAFV ++ G ++TE+DVK ++++QVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of CmoCh08G005640 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 676.4 bits (1744), Expect = 1.9e-194
Identity = 333/528 (63.07%), Postives = 422/528 (79.92%), Query Frame = 0

Query: 12  IFRSKLPDIRIPNHLPLHEYIFQNLPNYASRPCLINGATGHVYSYDDVQLTARRVASGLH 71
           IFRSKLPDI IPNHLPLH Y F+ L + + +PCLI G+TG  Y+Y +  L  RRVASGL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHLPF---VDFAADDVVALPY 191
            + + D++K+L EN   I   +   E CL +S L   DE++ PF   VD   DD  ALP+
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETN-PFQETVDIGGDDAAALPF 213

Query: 192 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYSLNSILLCG 251
           SSGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY K NDVILCVLP FHIYSLNS+LL  
Sbjct: 214 SSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNS 273

Query: 252 LRAGAAILIMQKFEIVSLLELVEKYKASIMPIVPPILLAIAKSSEFEKYDMSSVRVLKSG 311
           LR+GA +L+M KFEI +LL+L+++++ +I  +VPP+++A+AK+     YD+SSVR + SG
Sbjct: 274 LRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSG 333

Query: 312 GAPLGKKLEDTVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 371
            APLGK+L+D++R + P AILGQGYGMTEAGPVL+MSL FAKEP P K+G+CGTVVRNAE
Sbjct: 334 AAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAE 393

Query: 372 MKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDDD 431
           +K++  ET +SL  N  GEICIRG QIMK YLND E+T  TID+EGWLHTGDIG++D+DD
Sbjct: 394 LKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDD 453

Query: 432 ELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPVAFVVKANG 491
           E+FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   DE AGE+PVAFVV++NG
Sbjct: 454 EIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNG 513

Query: 492 GEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQL 537
            +ITEEDVK+++++QVVFYKRL +VFFV +IPK+PSGKILRK+L+++L
Sbjct: 514 NDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560