CmoCh08G005550 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G005550
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	Cmo_Chr08: 3410305 .. 3411552 (+)
RNA-Seq Expression	CmoCh08G005550
Synteny	CmoCh08G005550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACAGAAAATGGGCTTTCATTTTGCTTGCAAATGGCTTTTGTTAGCACTCCTGTTAGCCTCACCGGCGGTGAGCTTTATTGCAGGAGATGCAGAGAAAGACAGGGAGGAATGCACGCAGCAATTGGCTGGAATGGCGACGTGTCTAGCGTACGTGAGCGGTGACGCAAAGGCTCCAACGCCGGACTGTTGCAGTGGGCTGAAGGGCGTGCTTAAAAGCAACAAGAAATGCCTGTGCGTTATAGTGAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATTAATGTCACCCTTGCCTTGGGCCTGCCTGATGTCTGCCACGCCCCTGCCAATGTCTCCAATTGTCCTGGTAACTACTCCGCGTTCTTCGTGTTCTTCGTGTTCTTCGTGTTCTTCGTGTTCTTCTTTTAAATGGGGTTGTTGCAGCTCTATTAAACTTGCCGCCCAGTTCACCGGAGGCTCAAGTTTTCTATCAATTTGGCAAGGCGCAAAGCTCCTCTGCTGCTGCAGCTCCCATAAGTCCTTCTCCGGCCATTAGCTCCACTGGTAATTTGATGATCAATTTTCTACAGTTTTTTTTTAGTTTGGAAAGCAAAATTAAGGTGTTGTTCTTTAAAATTGCAGGTGGAGGAGGCAGTAGAAATGGAACAGATAAGGGGGAAGCTTACACCATTGGAAACAAATGGCTGCCATTGAAGAGTTTGGTTGTTGCAGCTCTTTTGGTTTTGTTTTTGTTGGATTCTTAAACTGTTTTGAGTATATTATTCATGATTTTATTGTGTTCTGTTCCAATATTAGTATTGTAATTCGTGTTTTTAAACGGTTGTTTGGAGTTGGAGATGGAATATATGTCCAGAGCGTCAAATTTGGGACCAATAAAGTAACATAGCGTTTACGTGATAACAACAATAAAATTTGTTTGAGGAGACGAGGACTCGTGATGCTTGACCACCCACAAAGCTCAAATACTTATGTTAGTTAGAGGGTTAGGAAAAGGTATTGAAATTGGATGAAAATATATCTATGTGTAAATTAGGGTTGACTAGGATCATACGAACAGAGAGAATGTTGGGTTCGGGTCTCTTGTGAGGTAGACCCTAGGGTTGGCCTCTGCCTCAAGGAACTTGGGCTTTGACCCAGTTTCGTTTTTACTTTCGTGTTTGGTGGTTCGGTTTCTTTTATATCACGATTTAGTTTAATTTGTATATCACTTTTTTTATTTTTTTTTATTTAGTTTTTTAATTTTTCG

mRNA sequence

CACAGAAAATGGGCTTTCATTTTGCTTGCAAATGGCTTTTGTTAGCACTCCTGTTAGCCTCACCGGCGGTGAGCTTTATTGCAGGAGATGCAGAGAAAGACAGGGAGGAATGCACGCAGCAATTGGCTGGAATGGCGACGTGTCTAGCGTACGTGAGCGGTGACGCAAAGGCTCCAACGCCGGACTGTTGCAGTGGGCTGAAGGGCGTGCTTAAAAGCAACAAGAAATGCCTGTGCGTTATAGTGAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATTAATGTCACCCTTGCCTTGGGCCTGCCTGATGTCTGCCACGCCCCTGCCAATGTCTCCAATTGTCCTGCTCTATTAAACTTGCCGCCCAGTTCACCGGAGGCTCAAGTTTTCTATCAATTTGGCAAGGCGCAAAGCTCCTCTGCTGCTGCAGCTCCCATAAGTCCTTCTCCGGCCATTAGCTCCACTGGTGGAGGAGGCAGTAGAAATGGAACAGATAAGGGGGAAGCTTACACCATTGGAAACAAATGGCTGCCATTGAAGAGTTTGGTTGTTGCAGCTCTTTTGGTTTTGTTTTTGTTGGATTCTTAAACTGTTTTGAGTATATTATTCATGATTTTATTGTGTTCTGTTCCAATATTAGTATTGTAATTCGTGTTTTTAAACGGTTGTTTGGAGTTGGAGATGGAATATATGTCCAGAGCGTCAAATTTGGGACCAATAAAGTAACATAGCGTTTACGTGATAACAACAATAAAATTTGTTTGAGGAGACGAGGACTCGTGATGCTTGACCACCCACAAAGCTCAAATACTTATGTTAGTTAGAGGGTTAGGAAAAGGTATTGAAATTGGATGAAAATATATCTATGTGTAAATTAGGGTTGACTAGGATCATACGAACAGAGAGAATGTTGGGTTCGGGTCTCTTGTGAGGTAGACCCTAGGGTTGGCCTCTGCCTCAAGGAACTTGGGCTTTGACCCAGTTTCGTTTTTACTTTCGTGTTTGGTGGTTCGGTTTCTTTTATATCACGATTTAGTTTAATTTGTATATCACTTTTTTTATTTTTTTTTATTTAGTTTTTTAATTTTTCG

Coding sequence (CDS)

ATGGGCTTTCATTTTGCTTGCAAATGGCTTTTGTTAGCACTCCTGTTAGCCTCACCGGCGGTGAGCTTTATTGCAGGAGATGCAGAGAAAGACAGGGAGGAATGCACGCAGCAATTGGCTGGAATGGCGACGTGTCTAGCGTACGTGAGCGGTGACGCAAAGGCTCCAACGCCGGACTGTTGCAGTGGGCTGAAGGGCGTGCTTAAAAGCAACAAGAAATGCCTGTGCGTTATAGTGAAAGACCGAAATGACCCTGACTTGGGGCTTCAGATTAATGTCACCCTTGCCTTGGGCCTGCCTGATGTCTGCCACGCCCCTGCCAATGTCTCCAATTGTCCTGCTCTATTAAACTTGCCGCCCAGTTCACCGGAGGCTCAAGTTTTCTATCAATTTGGCAAGGCGCAAAGCTCCTCTGCTGCTGCAGCTCCCATAAGTCCTTCTCCGGCCATTAGCTCCACTGGTGGAGGAGGCAGTAGAAATGGAACAGATAAGGGGGAAGCTTACACCATTGGAAACAAATGGCTGCCATTGAAGAGTTTGGTTGTTGCAGCTCTTTTGGTTTTGTTTTTGTTGGATTCTTAA

Protein sequence

MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSLVVAALLVLFLLDS

Homology

[Close]

BLAST of CmoCh08G005550 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.5e-45
Identity = 103/191 (53.93%), Postives = 128/191 (67.02%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V+  A D  KD+EECT+QL GMATCL YV G AK+PTPDCCSGLK VL SN
Sbjct: 13  IALIVVAMVVA-AADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSN 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSSPEAQVFYQF 131
           KKCLCVI++DRNDPDLGLQINV+LAL LP VCHA A+V+ CPALL+L P+SP+AQVFYQ 
Sbjct: 73  KKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVFYQL 132

Query: 132 GKAQSSSAAAA--------PISPSPAISSTGGGGSRNGTD---KGEAYTIGNKWLPLKSL 191
            K  + +  A+        PIS SP   S  G  S   T    +  A +   +WL L+  
Sbjct: 133 AKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGLE-- 192

[Close]

BLAST of CmoCh08G005550 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.8e-43
Identity = 95/191 (49.74%), Postives = 126/191 (65.97%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V     D  KD+EECT QL GMATCL YV G AK+PTPDCCSGLK V+ S+
Sbjct: 13  IALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSD 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSSPEAQVFYQF 131
            KCLC+I+++RNDPDLGLQ+NV+LAL LP VCHA A+++ CPALL+L P+SP+AQVFYQ 
Sbjct: 73  MKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQL 132

Query: 132 GK----AQSSSAAAAPISPSPAISSTGGGGSRNGTDK-------GEAYTIGNKWLPLKSL 191
            K      S+SA     S   ++SST G  + N + +         A +   +WL L+  
Sbjct: 133 AKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWLGLE-- 192

[Close]

BLAST of CmoCh08G005550 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.7e-28
Identity = 66/181 (36.46%), Postives = 109/181 (60.22%), Query Frame = 0


Query: 10  LLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLK 69
           L + +++ S  + F   D  +DREECT QL  ++TC+ YV GDAKAPT DCC+G   V++
Sbjct: 8   LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 67

Query: 70  SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPA-NVSNCPALLNLPPSSPEAQVF 129
            ++KC+C++V+D++DP LG++IN TLA  LP  CH  A N+++C ++L+LP +S  A+ F
Sbjct: 68  KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEF 127

Query: 130 YQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSLVVAALLVL 189
              G+ + +  + +P      I   G GG +    K   +     WL ++ L+   + ++
Sbjct: 128 ENLGRIEDNYNSTSPTQ----IHKDGTGGGKAEPVKSNGWK-EKSWLGVELLIYLLVSLI 183

[Close]

BLAST of CmoCh08G005550 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.6e-23
Identity = 57/144 (39.58%), Postives = 87/144 (60.42%), Query Frame = 0


Query: 11  LLALLLASPAVSF--IAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVL 70
           +LAL + + AV F  +  +  +D + C   ++ + +CL +V+  AKAP   CCS LK  +
Sbjct: 13  MLALCI-TVAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKI 72

Query: 71  K--SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSSPEAQ 130
                +KCLC +VKDR+DP LG +++   A+ LP  CH PAN+S CP LL+L P SP +Q
Sbjct: 73  DKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQ 132

Query: 131 VFYQFGKAQSSSAAAAPISPSPAI 151
           +F QF ++ S +     +S S +I
Sbjct: 133 IFKQFTESSSQTVGHKAVSTSSSI 155

[Close]

BLAST of CmoCh08G005550 vs. ExPASy Swiss-Prot
Match: Q9C9B1 (Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3702 GN=LTPG10 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.8e-09
Identity = 56/191 (29.32%), Postives = 81/191 (42.41%), Query Frame = 0


Query: 11  LLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKS 70
           LL  LL S +  F+     +    C  +L  +A C  +V G A+ P   CC  L  +   
Sbjct: 6   LLITLLISLSAFFLRMVLAQVPATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQ 65

Query: 71  NKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNC-----------PALLNLP 130
              CLC+ + + +       IN TLAL LP +C+ PAN S C            + +  P
Sbjct: 66  EATCLCLFLNNTSTLSPAFPINQTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPP 125

Query: 131 PSSPEAQVFYQFGKAQSSSAA--AAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPL 189
           PSS       Q  K  S  AA   A ++P P      G G ++   K E   I      L
Sbjct: 126 PSSSTGSQISQGAKNNSRVAATPVAQMAPRPTSFMGLGYGLKSSGSKSE---IQLTIFAL 185

[Close]

BLAST of CmoCh08G005550 vs. ExPASy TrEMBL
Match: A0A6J1H6P3 (protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 8.0e-103
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180
           SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180

Query: 181 VVAALLVLFLLDS 194
           VVAALLVLFLLDS
Sbjct: 181 VVAALLVLFLLDS 193

[Close]

BLAST of CmoCh08G005550 vs. ExPASy TrEMBL
Match: A0A6J1KZ76 (protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.4e-99
Identity = 185/193 (95.85%), Postives = 188/193 (97.41%), Query Frame = 0


Query: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHF CKWLLL LLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLTLLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVS CPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSTCPALLNLPP 120

Query: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180
           SSPEAQVFYQFGKAQSSSAAAAPISPSPAI+STGGGGSRNGTDKGEAYT+GNKWLPLK+L
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTVGNKWLPLKTL 180

Query: 181 VVAALLVLFLLDS 194
            V ALLVLFLLDS
Sbjct: 181 FVGALLVLFLLDS 193

[Close]

BLAST of CmoCh08G005550 vs. ExPASy TrEMBL
Match: A0A6J1D8S3 (protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.8e-77
Identity = 155/196 (79.08%), Postives = 165/196 (84.18%), Query Frame = 0


Query: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           M FHFA K LLL LLL SPAV  +  DAEKD+EECTQQLAGMATCL YVSGDAK+PTPDC
Sbjct: 1   MDFHFAFKLLLLTLLLTSPAVRLVTADAEKDKEECTQQLAGMATCLPYVSGDAKSPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           C+GLK VLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPD CHA ANVSNCPALLNLPP
Sbjct: 61  CNGLKEVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDSCHASANVSNCPALLNLPP 120

Query: 121 SSPEAQVFYQFGKAQSSSA---AAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPL 180
           +SPEAQVFYQ GK+ SSSA   AAAPISPS   +ST GGGSR G DKGEA   GNKWLPL
Sbjct: 121 NSPEAQVFYQLGKSNSSSAAASAAAPISPSSTTNSTVGGGSRIGPDKGEACYRGNKWLPL 180

Query: 181 KSLVVAALLVLFLLDS 194
           ++LV  ALL+ F LDS
Sbjct: 181 ENLVFGALLLCFFLDS 196

[Close]

BLAST of CmoCh08G005550 vs. ExPASy TrEMBL
Match: A0A1S3CDS0 (protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 4.8e-63
Identity = 141/194 (72.68%), Postives = 152/194 (78.35%), Query Frame = 0


Query: 2   GFHFACKWLLLALLLASP-AVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 61
           GF    KW LL LLL SP +V F+ GD +KD+EECT QLAGMATCL YVSGDAK PTPDC
Sbjct: 3   GFRLCFKWFLLMLLLRSPSSVRFVGGDDKKDKEECTPQLAGMATCLPYVSGDAKTPTPDC 62

Query: 62  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 121
           CSGLK VLK+NKKCLCVIVKDRNDPDLGLQINVTLALGLPD+CHA ANVSNCPALLNLP 
Sbjct: 63  CSGLKEVLKNNKKCLCVIVKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNLPS 122

Query: 122 SSPEAQVFYQFGKAQSSSA-AAAP-ISP-SPA-ISSTGGGGSRNGTDKGEAYTIGNKWLP 181
           +S EAQVFYQ GK +SSSA A AP ISP SPA ISST GGGS     K   Y IG KW+P
Sbjct: 123 NSSEAQVFYQLGKGKSSSALAPAPIISPSSPATISSTVGGGS----TKSGGYKIGKKWVP 182

Query: 182 LKSLVVAALLVLFL 191
            + LVV   L+L L
Sbjct: 183 SEILVVVVALLLLL 192

[Close]

BLAST of CmoCh08G005550 vs. ExPASy TrEMBL
Match: A0A0A0K6P6 (Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 6.4e-60
Identity = 133/192 (69.27%), Postives = 155/192 (80.73%), Query Frame = 0


Query: 5   FACKWL-LLALLLASPA-VSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCS 64
           F+ KWL L+ LLL SP+ V F+ GD +KDREECT QLAGMATCL YVSGDAKAPTPDCCS
Sbjct: 7   FSFKWLWLIMLLLRSPSWVRFVGGDDKKDREECTPQLAGMATCLPYVSGDAKAPTPDCCS 66

Query: 65  GLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSS 124
           GLK VL+++KKCLCVIV+DRNDPDLGLQINVTLAL LPD+CHA ANVSNCPALLN+P +S
Sbjct: 67  GLKEVLQNDKKCLCVIVRDRNDPDLGLQINVTLALSLPDICHATANVSNCPALLNMPSNS 126

Query: 125 PEAQVFYQFGKAQSSSA-AAAPI---SPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLK 184
            +AQVFYQ GK +SSSA A AP+   S SP I+ST GGGS++G + GE Y  G K + L+
Sbjct: 127 SDAQVFYQLGKGKSSSALAPAPMLSPSSSPTINSTVGGGSKSGAE-GEGYKRGKKRVALE 186

Query: 185 SLVVAALLVLFL 191
            LVV A L+L L
Sbjct: 187 ILVVVAPLLLLL 197

[Close]

BLAST of CmoCh08G005550 vs. NCBI nr
Match: XP_022959693.1 (protein YLS3-like [Cucurbita moschata])

HSP 1 Score: 382.9 bits (982), Expect = 1.7e-102
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180
           SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180

Query: 181 VVAALLVLFLLDS 194
           VVAALLVLFLLDS
Sbjct: 181 VVAALLVLFLLDS 193

[Close]

BLAST of CmoCh08G005550 vs. NCBI nr
Match: XP_023513729.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 379.0 bits (972), Expect = 2.4e-101
Identity = 190/193 (98.45%), Postives = 192/193 (99.48%), Query Frame = 0


Query: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHF CKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180
           +SPEAQVFYQFGKAQSSSAAAAPISPSPAI+STGGGGSRNGTDKGEAYTIGNKWLPLKSL
Sbjct: 121 NSPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180

Query: 181 VVAALLVLFLLDS 194
           VVAALLVLFLLDS
Sbjct: 181 VVAALLVLFLLDS 193

[Close]

BLAST of CmoCh08G005550 vs. NCBI nr
Match: XP_023004343.1 (protein YLS3-like [Cucurbita maxima])

HSP 1 Score: 371.3 bits (952), Expect = 5.0e-99
Identity = 185/193 (95.85%), Postives = 188/193 (97.41%), Query Frame = 0


Query: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHF CKWLLL LLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLTLLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVS CPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSTCPALLNLPP 120

Query: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180
           SSPEAQVFYQFGKAQSSSAAAAPISPSPAI+STGGGGSRNGTDKGEAYT+GNKWLPLK+L
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAINSTGGGGSRNGTDKGEAYTVGNKWLPLKTL 180

Query: 181 VVAALLVLFLLDS 194
            V ALLVLFLLDS
Sbjct: 181 FVGALLVLFLLDS 193

[Close]

BLAST of CmoCh08G005550 vs. NCBI nr
Match: KAG6593339.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 367.9 bits (943), Expect = 5.5e-98
Identity = 187/193 (96.89%), Postives = 189/193 (97.93%), Query Frame = 0


Query: 1   MGFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60
           MGFHF CKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC
Sbjct: 1   MGFHFGCKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDC 60

Query: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120
           CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP
Sbjct: 61  CSGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPP 120

Query: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSL 180
           SSPEAQVFYQFGKAQSSSAAAAPISPSPAI+ST GGGSRNGTDKGEAYTIGNKWLPLK+L
Sbjct: 121 SSPEAQVFYQFGKAQSSSAAAAPISPSPAINST-GGGSRNGTDKGEAYTIGNKWLPLKTL 180

Query: 181 VVAALLVLFLLDS 194
           VV ALLVL LLDS
Sbjct: 181 VVGALLVLSLLDS 192

[Close]

BLAST of CmoCh08G005550 vs. NCBI nr
Match: XP_038899115.1 (non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida])

HSP 1 Score: 307.0 bits (785), Expect = 1.2e-79
Identity = 155/194 (79.90%), Postives = 166/194 (85.57%), Query Frame = 0


Query: 2   GFHFACKWLLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCC 61
           GFHF  KWLLL LLL SP VSF+ GD EKD+EECTQQLAGMATCL YVSG AKAPTPDCC
Sbjct: 3   GFHFTWKWLLLTLLLTSPTVSFVGGDDEKDKEECTQQLAGMATCLPYVSGGAKAPTPDCC 62

Query: 62  SGLKGVLKSNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPS 121
           SGLK VLK+NKKCLCVI+KDRNDPDLGLQINVTLALGLPD+CHA ANVSNCPALLNLPP+
Sbjct: 63  SGLKEVLKNNKKCLCVIIKDRNDPDLGLQINVTLALGLPDICHATANVSNCPALLNLPPN 122

Query: 122 SPEAQVFYQFGKAQSSSAAA--APISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKS 181
           S EAQVFYQ GK  SSS+A   APISPSP I+ST GGGSR G DKG AY IG KWLPL++
Sbjct: 123 SAEAQVFYQLGKGNSSSSAVAPAPISPSPTINSTVGGGSRIGPDKGGAYNIGKKWLPLET 182

Query: 182 LVVAALLVLFLLDS 194
           L V ALL+LF LDS
Sbjct: 183 LAVGALLLLFWLDS 196

[Close]

BLAST of CmoCh08G005550 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 1.8e-46
Identity = 103/191 (53.93%), Postives = 128/191 (67.02%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V+  A D  KD+EECT+QL GMATCL YV G AK+PTPDCCSGLK VL SN
Sbjct: 13  IALIVVAMVVA-AADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVLNSN 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSSPEAQVFYQF 131
           KKCLCVI++DRNDPDLGLQINV+LAL LP VCHA A+V+ CPALL+L P+SP+AQVFYQ 
Sbjct: 73  KKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVFYQL 132

Query: 132 GKAQSSSAAAA--------PISPSPAISSTGGGGSRNGTD---KGEAYTIGNKWLPLKSL 191
            K  + +  A+        PIS SP   S  G  S   T    +  A +   +WL L+  
Sbjct: 133 AKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGLE-- 192

[Close]

BLAST of CmoCh08G005550 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 1.3e-44
Identity = 95/191 (49.74%), Postives = 126/191 (65.97%), Query Frame = 0


Query: 12  LALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLKSN 71
           +AL++ +  V     D  KD+EECT QL GMATCL YV G AK+PTPDCCSGLK V+ S+
Sbjct: 13  IALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSD 72

Query: 72  KKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSSPEAQVFYQF 131
            KCLC+I+++RNDPDLGLQ+NV+LAL LP VCHA A+++ CPALL+L P+SP+AQVFYQ 
Sbjct: 73  MKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQL 132

Query: 132 GK----AQSSSAAAAPISPSPAISSTGGGGSRNGTDK-------GEAYTIGNKWLPLKSL 191
            K      S+SA     S   ++SST G  + N + +         A +   +WL L+  
Sbjct: 133 AKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWLGLE-- 192

[Close]

BLAST of CmoCh08G005550 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-29
Identity = 66/181 (36.46%), Postives = 109/181 (60.22%), Query Frame = 0


Query: 10  LLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLK 69
           L + +++ S  + F   D  +DREECT QL  ++TC+ YV GDAKAPT DCC+G   V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 70  SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPA-NVSNCPALLNLPPSSPEAQVF 129
            ++KC+C++V+D++DP LG++IN TLA  LP  CH  A N+++C ++L+LP +S  A+ F
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEF 170

Query: 130 YQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSLVVAALLVL 189
              G+ + +  + +P      I   G GG +    K   +     WL ++ L+   + ++
Sbjct: 171 ENLGRIEDNYNSTSPTQ----IHKDGTGGGKAEPVKSNGWK-EKSWLGVELLIYLLVSLI 226

[Close]

BLAST of CmoCh08G005550 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-28
Identity = 67/181 (37.02%), Postives = 107/181 (59.12%), Query Frame = 0


Query: 10  LLLALLLASPAVSFIAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVLK 69
           L + +++ S  + F   D  +DREECT QL  ++TC+ YV GDAKAPT DCC+G   V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 70  SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPA-NVSNCPALLNLPPSSPEAQVF 129
            ++KC+C++V+D++DP LG++IN TLA  LP  CH  A N+++C ++L+LP +S  A+ F
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEF 170

Query: 130 YQFGKAQSSSAAAAPISPSPAISSTGGGGSRNGTDKGEAYTIGNKWLPLKSLVVAALLVL 189
              G+ + +       S SP     GGG +      G        WL ++ L+   + ++
Sbjct: 171 ENLGRIEDNYN-----STSPTQIHKGGGKAEPVKSNGWK---EKSWLGVELLIYLLVSLI 223

[Close]

BLAST of CmoCh08G005550 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.5e-24
Identity = 57/144 (39.58%), Postives = 87/144 (60.42%), Query Frame = 0


Query: 11  LLALLLASPAVSF--IAGDAEKDREECTQQLAGMATCLAYVSGDAKAPTPDCCSGLKGVL 70
           +LAL + + AV F  +  +  +D + C   ++ + +CL +V+  AKAP   CCS LK  +
Sbjct: 13  MLALCI-TVAVMFLGVRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKI 72

Query: 71  K--SNKKCLCVIVKDRNDPDLGLQINVTLALGLPDVCHAPANVSNCPALLNLPPSSPEAQ 130
                +KCLC +VKDR+DP LG +++   A+ LP  CH PAN+S CP LL+L P SP +Q
Sbjct: 73  DKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQ 132

Query: 131 VFYQFGKAQSSSAAAAPISPSPAI 151
           +F QF ++ S +     +S S +I
Sbjct: 133 IFKQFTESSSQTVGHKAVSTSSSI 155

The following BLAST results are available for this feature:

BLAST of CmoCh08G005550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64865	2.5e-45	53.93	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
O64864	1.8e-43	49.74	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
F4I082	1.7e-28	36.46	Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37...	[more]
Q9M2G1	3.6e-23	39.58	Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3...	[more]
Q9C9B1	3.8e-09	29.32	Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmoCh08G005550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6P3	8.0e-103	100.00	protein YLS3-like OS=Cucurbita moschata OX=3662 GN=LOC111460690 PE=3 SV=1	[more]
A0A6J1KZ76	2.4e-99	95.85	protein YLS3-like OS=Cucurbita maxima OX=3661 GN=LOC111497688 PE=3 SV=1	[more]
A0A6J1D8S3	5.8e-77	79.08	protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111018659 PE=3 SV=1	[more]
A0A1S3CDS0	4.8e-63	72.68	protein YLS3-like OS=Cucumis melo OX=3656 GN=LOC103499877 PE=4 SV=1	[more]
A0A0A0K6P6	6.4e-60	69.27	Non-specific lipid-transfer protein OS=Cucumis sativus OX=3659 GN=Csa_7G430130 P...	[more]

BLAST of CmoCh08G005550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022959693.1	1.7e-102	100.00	protein YLS3-like [Cucurbita moschata]	[more]
XP_023513729.1	2.4e-101	98.45	protein YLS3-like [Cucurbita pepo subsp. pepo]	[more]
XP_023004343.1	5.0e-99	95.85	protein YLS3-like [Cucurbita maxima]	[more]
KAG6593339.1	5.5e-98	96.89	Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025687.1 Prot...	[more]
XP_038899115.1	1.2e-79	79.90	non-specific lipid transfer protein GPI-anchored 13 [Benincasa hispida]	[more]

BLAST of CmoCh08G005550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44300.1	1.8e-46	53.93	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	1.3e-44	49.74	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.1	1.2e-29	36.46	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.2	1.3e-28	37.02	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G58550.1	2.5e-24	39.58	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000528	Plant non-specific lipid-transfer protein/Par allergen	PRINTS	PR00382	LIPIDTRNSFER	coord: 90..107 score: 28.63 coord: 55..69 score: 33.23 coord: 34..50 score: 28.33
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 35..112 e-value: 1.3E-5 score: 34.7
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 16..112 e-value: 3.6E-14 score: 52.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 32..112 e-value: 2.9E-14 score: 54.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 34..113
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 140..160
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 140..159
None	No IPR available	PANTHER	PTHR33044:SF192	PROTEIN YLS3-LIKE ISOFORM X1	coord: 23..186
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 42..104 e-value: 6.33163E-16 score: 67.0666
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 23..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G005550.1	CmoCh08G005550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008643	carbohydrate transport
biological_process	GO:0006869	lipid transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008289	lipid binding
molecular_function	GO:0015293	symporter activity