CmoCh08G004780 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh08G004780
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	gibberellin 2-beta-dioxygenase 2-like
Location	Cmo_Chr08: 2956802 .. 2958077 (+)
RNA-Seq Expression	CmoCh08G004780
Synteny	CmoCh08G004780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTTTCCGACAGCCATGAGATCCAAAAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTTGAGGCCTGCGAGCACTTTGGCATCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCTAATCTTGAACGCCAAGCCTTTGATTTCTTTGGCCAAACAGCGCCCGAGAAGCGCCGAGCTGGCCCTGCTGCCCCCTTTGGCTATGGCTGCAAAGCCATTGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCGCTCCGACCCTGCATCCATTGCTGAAACCTCCGCCTCCATCTCCAACAGCCCTCTTGGTTTTAGGTACTGCTTAGCTAGAAGAAAAAAATTGAGTTTTTAACTGAAACATTGATTTATGTCGACATGTCGTCTGATATCTTAATTTGATGCAGCTATGTTGTGAATGAGTACGTTAAATGTGTAAGAGAATTAGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCAGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAAGATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTGGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTATGTCCCGACCCGTCTGCGTTCTACGTCTTGATTGGTGATGCTTTTCAGGTAAATTCTATGTCATTCATCTGTAGATCAAAGTATAAAAACTATTATATAAGATGCACGTAACAAGTCTAGTGTCAAACGAGACGTCGATTTTTGTACTTAAAACCTAAAGAACGAAACATGATCACGAACATTAGAAGCAAAAACATTTACAAAATGGGTTATTGAGACTTGTTCTTGTTTTTGATTAGGCAATGACAAATGATAGATTTGTAAGCGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCACCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCCGACTCTCGTCTTAATCTCTTCAAAGCTTCTTAA

mRNA sequence

ATGCCTTTTCCGACAGCCATGAGATCCAAAAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTTGAGGCCTGCGAGCACTTTGGCATCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCTAATCTTGAACGCCAAGCCTTTGATTTCTTTGGCCAAACAGCGCCCGAGAAGCGCCGAGCTGGCCCTGCTGCCCCCTTTGGCTATGGCTGCAAAGCCATTGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCGCTCCGACCCTGCATCCATTGCTGAAACCTCCGCCTCCATCTCCAACAGCCCTCTTGGTTTTAGCTATGTTGTGAATGAGTACGTTAAATGTGTAAGAGAATTAGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCAGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAAGATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTGGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTATGTCCCGACCCGTCTGCGTTCTACGTCTTGATTGGTGATGCTTTTCAGGCAATGACAAATGATAGATTTGTAAGCGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCACCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCCGACTCTCGTCTTAATCTCTTCAAAGCTTCTTAA

Coding sequence (CDS)

ATGCCTTTTCCGACAGCCATGAGATCCAAAAAGACAAAGGCCGTCGGAATTCCGACCATAGATCTCTCCGAAAGCAGAGCAAGAACTTCCAAATCCATTGTTGAGGCCTGCGAGCACTTTGGCATCTTCAAAGTCATCAACCATGGAGTTTCTGACCATGCCATTGCTAATCTTGAACGCCAAGCCTTTGATTTCTTTGGCCAAACAGCGCCCGAGAAGCGCCGAGCTGGCCCTGCTGCCCCCTTTGGCTATGGCTGCAAAGCCATTGGCCCCAACGGCGACATCGGCGACCTCGAGTATATCCTTCTTCGCTCCGACCCTGCATCCATTGCTGAAACCTCCGCCTCCATCTCCAACAGCCCTCTTGGTTTTAGCTATGTTGTGAATGAGTACGTTAAATGTGTAAGAGAATTAGCATGTGAGATTCTTGATCTGGTGGGGGAGGGTCTATGGTTACCAAACAAGTCTGTCTTCAGTAACCTCATCAGAGACGTCCAAAGTGATTCAGTTTTGAGGATCAATTACTACCCTGCAGCAGTCAAGCACACTGAAAAAAGTAGGGAACTTTCATCAAAATCCCACCTTTCAACTAACAAGATTGGATTTGGGGAGCATTCTGACCCTCAGATCTTGACCATCTTGAGATCGAACGATGTGGATGGCCTCCAAATCTCCTTGCACGACGGGTTGTGGACACCTGTATGTCCCGACCCGTCTGCGTTCTACGTCTTGATTGGTGATGCTTTTCAGGCAATGACAAATGATAGATTTGTAAGCGTGAGGCATAGGGCAATGGCAAACAACTCAAAGAAGGCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCATGGATAGCTCCTCCATCAGATATGATGCTCACCAAGGACCAATCTCTATATAAGCCTTTCACTTGGGCTCAATACAAGAAAGCTGCTTATTCTCTTAGATTAGCCGACTCTCGTCTTAATCTCTTCAAAGCTTCTTAA

Protein sequence

MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNSPLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS

Homology

[Close]

BLAST of CmoCh08G004780 vs. ExPASy Swiss-Prot
Match: Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 6.2e-108
Identity = 192/331 (58.01%), Postives = 248/331 (74.92%), Query Frame = 0


Query: 1   MPFPTAM-RSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLE 60
           +P PT+M R+KKTKAVGIPTIDLS  R++ S+ +V+ACE +G FKV+NH V    I+ L+
Sbjct: 3   VPSPTSMIRTKKTKAVGIPTIDLSLERSQLSELVVKACEEYGFFKVVNHSVPKEVISRLD 62

Query: 61  RQAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASIS- 120
            +  +FF + + EKR+AG + PFGYGCK IGPNGD G+LEY+LL S+P SI+E S +I+ 
Sbjct: 63  EEGIEFFSKNSSEKRQAGTSTPFGYGCKNIGPNGDKGELEYLLLHSNPISISERSKTIAK 122

Query: 121 NSPLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPA 180
           + P+ FS +VN+Y+K V++L CEIL+L  EGLW+P+KS  S +I+D  SDS+LRIN+YP 
Sbjct: 123 DHPIKFSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKIIKDEHSDSLLRINHYPP 182

Query: 181 AVKHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDP 240
             K    + + S   + + N IGFGEHSDPQILTILRSN+V GLQIS H GLW PV PDP
Sbjct: 183 VKKLGNDNWDPSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWIPVPPDP 242

Query: 241 SAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTK 300
           S FYV++GDA Q +TN RFVSVRHR + N + K RMS+MYF APPLN  I+P S M+   
Sbjct: 243 SEFYVMVGDALQVLTNGRFVSVRHRVLTNTT-KPRMSMMYFAAPPLNWLISPLSKMVTAH 302

Query: 301 DQSLYKPFTWAQYKKAAYSLRLADSRLNLFK 330
              LY+PFTWAQYK+AAY+LRL D+RL+ FK
Sbjct: 303 SPCLYRPFTWAQYKQAAYALRLGDTRLDQFK 332

[Close]

BLAST of CmoCh08G004780 vs. ExPASy Swiss-Prot
Match: Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 2.9e-89
Identity = 168/327 (51.38%), Postives = 214/327 (65.44%), Query Frame = 0


Query: 5   TAMRSKKTKAVGIPTIDLS-ESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAF 64
           T  ++  +     P ID S   R++ S+ IV+ACE  G FKVINHGV    I   E +  
Sbjct: 12  TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71

Query: 65  DFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASIS-NSPL 124
           +FF +   +K RAGPA+PFGYGCK IG NGD+G+LEY+LL ++P ++A+ S +IS + P 
Sbjct: 72  EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131

Query: 125 GFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKH 184
            FS   N+Y++ VR+LACEI+DL  E LW    S  S LIRDV+SDS+LR+N+YP A   
Sbjct: 132 KFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA--- 191

Query: 185 TEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFY 244
                     +     +IGFGEHSDPQILT+LRSNDVDGL+I   DGLW P+  DP+ F+
Sbjct: 192 --------PYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFF 251

Query: 245 VLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSL 304
           VL+GD  QA+TN RF SVRHR +AN +KK RMS MYF APPL A I+P   M+  ++   
Sbjct: 252 VLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRR 311

Query: 305 YKPFTWAQYKKAAYSLRLADSRLNLFK 330
           Y  FTW  YKKA YSLRL   RL  FK
Sbjct: 312 YNSFTWGDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of CmoCh08G004780 vs. ExPASy Swiss-Prot
Match: Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.0e-78
Identity = 165/326 (50.61%), Postives = 218/326 (66.87%), Query Frame = 0


Query: 9   SKKTKAV--GIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFF 68
           S+K  AV   IP ID+S+ R++ S  IV+ACE  G FKVINHGV    I+ +E+++ +FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 69  GQTAPEKRRAGPA-APFGYGCKAIGPNGDIGDLEYILLR-SDPASIAETSASISNSPLGF 128
            + A EK+   P   PF YG + IG NGD G++EY+L   +DPA           S L F
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA---------FRSQLSF 124

Query: 129 SYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTE 188
           S  VN Y++ V++LA EILDL  EGL +P  S FS LI  V SDSVLR+N+YP + +   
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 189 KSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVL 248
           ++  LS +S +S  ++GFGEH+DPQILT+LRSN V GLQ+S  DG+W  V PDPSAF V 
Sbjct: 185 EA-NLSDQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVN 244

Query: 249 IGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ-SLY 308
           +GD  Q MTN RF+SVRHRA+    +++R+S  YF  PPL A I P S M++T +Q  LY
Sbjct: 245 VGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLY 304

Query: 309 KPFTWAQYKKAAYSLRLADSRLNLFK 330
           + FTW +YKK AYSLRL DSRL++F+
Sbjct: 305 QTFTWGEYKKRAYSLRLEDSRLDMFR 317

[Close]

BLAST of CmoCh08G004780 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.3e-77
Identity = 146/314 (46.50%), Postives = 210/314 (66.88%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRA 76
           IP ++L++  A+T   IV+ACE FG FKV+NHGV    +  LE++A  FFG     K RA
Sbjct: 31  IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRA 90

Query: 77  GPAAPFGYGCKAIGPNGDIGDLEYILLRSDP-ASIAETSASISNSPLGFSYVVNEYVKCV 136
           GP  P+GYG K IGPNGD+G +EY+LL ++P  S  +TSA    +P  F   V EY+K +
Sbjct: 91  GPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEI 150

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           +E++ ++L++V E L +  +   S ++RD +SDS LR+N+YPAA +  EK          
Sbjct: 151 KEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEK---------- 210

Query: 197 STNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTND 256
              K+GFGEH+DPQI+++LRSN+  GLQI + DG W  V PD S+F++ +GDA Q MTN 
Sbjct: 211 -MVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNG 270

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAA 316
           RF SV+HR +A +++++R+S++YFG PPL+  IAP   ++  +D  LYK FTW+QYK +A
Sbjct: 271 RFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSA 330

Query: 317 YSLRLADSRLNLFK 330
           Y  +L D RL LF+
Sbjct: 331 YKSKLGDYRLGLFE 330

[Close]

BLAST of CmoCh08G004780 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.8e-76
Identity = 147/317 (46.37%), Postives = 204/317 (64.35%), Query Frame = 0


Query: 16  GIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFFGQTAPEKRR 75
           GIP +DL+   A+    IV AC  FG FK++NHGV    +ANLE +A  FF ++  EK R
Sbjct: 26  GIPVVDLTHPDAK--NLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDR 85

Query: 76  AGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASI-SNSPLGFSYVVNEYVKC 135
           AGP  PFGYG K IGPNGD+G +EY+LL ++P  I+  S  I   +P  F  VV  Y+  
Sbjct: 86  AGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVENYITA 145

Query: 136 VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSH 195
           V+ +   +L+L+ EGL +  ++  S L++D +SDS  R+N+YP                 
Sbjct: 146 VKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPC----------PEVQA 205

Query: 196 LSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTN 255
           L+ N +GFGEH+DPQI+++LRSN   GLQI L DG W  V PD ++F++ +GDA Q MTN
Sbjct: 206 LNRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTN 265

Query: 256 DRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKA 315
            RF SV+HR +A+ + K+R+S++YFG P L+  IAP   +ML  ++ LYK FTW +YKKA
Sbjct: 266 GRFKSVKHRVLADTT-KSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKA 325

Query: 316 AYSLRLADSRLNLFKAS 332
           AY+ RLAD+RL  F+ S
Sbjct: 326 AYTSRLADNRLAPFQKS 329

[Close]

BLAST of CmoCh08G004780 vs. ExPASy TrEMBL
Match: A0A6J1H8N0 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460653 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 9.6e-189
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of CmoCh08G004780 vs. ExPASy TrEMBL
Match: A0A6J1KWQ4 (gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.9e-183
Identity = 321/331 (96.98%), Postives = 324/331 (97.89%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MPFPTA RSKKTKAVGIPTIDLSESRARTSKSIVEAC+HFG FKVINHGVSDHAIANLER
Sbjct: 1   MPFPTATRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILL SDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PL FSYVVNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTN IGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FY+LIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLHLFKAS 331

[Close]

BLAST of CmoCh08G004780 vs. ExPASy TrEMBL
Match: A0A6J1GRC3 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456420 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.6e-154
Identity = 278/331 (83.99%), Postives = 297/331 (89.73%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MP PTAMR+KKTKAVGIPTIDLS++R  TSKSI+ AC HFG FK+INHGVS HAI+ LER
Sbjct: 1   MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
            AFDFF +TAPEK RAGPAAPFGYG KAIGPNGD GDLEY+LLRSDPAS+ +TS SISN 
Sbjct: 61  HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           P  FSYVVNEYVK V++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYP AV
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYP-AV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTE + ELSSKS+ S N+IGFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTE-NWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDA QAMTN RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAP S+M+L   Q
Sbjct: 241 FYVLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

[Close]

BLAST of CmoCh08G004780 vs. ExPASy TrEMBL
Match: A0A6J1JXB5 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488342 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.2e-152
Identity = 275/331 (83.08%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MP PTAMR+KKT+AVGIPTIDLS++R  TSKSI+ AC HFG FK+INHGVS HAI+ LE 
Sbjct: 1   MPCPTAMRTKKTEAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLEC 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFF +TAPEK RAGPAAPFGYG KAIGPNGD GDLEY+LLRSDPAS+ +TS SISN 
Sbjct: 61  QAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           P  FSYVVNEYVK V++L CEILDLVGEGLWLPNKSVFS+LIRDV SDSVLRINYYP AV
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYP-AV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTE + ELSSKS+ S N+IGFGEHSDPQILTILRSNDVDGLQISL DGLW PVCPDPSA
Sbjct: 181 KHTE-NWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDA QAMTN RFVSVRHRAMAN+SKKARMSIMYFGAPPLNAWIAP S+M+    Q
Sbjct: 241 FYVLIGDALQAMTNGRFVSVRHRAMANSSKKARMSIMYFGAPPLNAWIAPLSEMIPPNHQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329

[Close]

BLAST of CmoCh08G004780 vs. ExPASy TrEMBL
Match: A0A6J1DEJ9 (gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.5e-149
Identity = 271/332 (81.63%), Postives = 292/332 (87.95%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MP PTAMR+KK KAVGIPTIDLSESRARTSK+IVEACE FG FK++NHGVSD AIA LER
Sbjct: 1   MPCPTAMRTKKAKAVGIPTIDLSESRARTSKAIVEACEEFGFFKLVNHGVSDQAIAGLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           Q FDFF + APEKRRAGPA PFGYGCK IGPNGD G+LEY+LLRSDPA+IAETS SIS+ 
Sbjct: 61  QGFDFFAKPAPEKRRAGPADPFGYGCKTIGPNGDTGELEYLLLRSDPAAIAETSLSISSD 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           P  FSY VNEY+K V+ELACEILDLVGEGLWLPNKS+ S LIRDVQSDSVLRIN+YP AV
Sbjct: 121 PPSFSYAVNEYIKSVKELACEILDLVGEGLWLPNKSIISGLIRDVQSDSVLRINHYP-AV 180

Query: 181 KHTEKSRELSSKSHLSTN-KIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPS 240
           KHTE   ELSSKSH S N +IGFGEHSDPQILTILRSNDV GLQISL  GLW PVCPDPS
Sbjct: 181 KHTE-DWELSSKSHPSNNTRIGFGEHSDPQILTILRSNDVGGLQISLPGGLWVPVCPDPS 240

Query: 241 AFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKD 300
           AFYVLIGDAFQAM+N RFVSVRHRAMANNS K RMS+MYFGAPPLNAWIAP S++ML   
Sbjct: 241 AFYVLIGDAFQAMSNGRFVSVRHRAMANNSNKPRMSMMYFGAPPLNAWIAPLSELMLPHH 300

Query: 301 QSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
            S+Y+PFTW QYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 PSMYQPFTWGQYKKAAYSLRLADSRLDLFKAS 330

[Close]

BLAST of CmoCh08G004780 vs. NCBI nr
Match: XP_022959634.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata])

HSP 1 Score: 669.1 bits (1725), Expect = 2.0e-188
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of CmoCh08G004780 vs. NCBI nr
Match: KAG6593256.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 661.8 bits (1706), Expect = 3.2e-186
Identity = 328/331 (99.09%), Postives = 328/331 (99.09%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLE 
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPA PFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of CmoCh08G004780 vs. NCBI nr
Match: KAG7025608.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661.4 bits (1705), Expect = 4.2e-186
Identity = 328/331 (99.09%), Postives = 328/331 (99.09%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPA PFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of CmoCh08G004780 vs. NCBI nr
Match: XP_023514766.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.4 bits (1700), Expect = 1.6e-185
Identity = 326/331 (98.49%), Postives = 328/331 (99.09%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEAC+HFGIFKVINHGVSDHAIANLER
Sbjct: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGIFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEK RAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKHRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PLGFSYVVNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLGFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTNK+GFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNKVGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331

[Close]

BLAST of CmoCh08G004780 vs. NCBI nr
Match: XP_023004594.1 (gibberellin 2-beta-dioxygenase 2 [Cucurbita maxima])

HSP 1 Score: 649.4 bits (1674), Expect = 1.6e-182
Identity = 321/331 (96.98%), Postives = 324/331 (97.89%), Query Frame = 0


Query: 1   MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
           MPFPTA RSKKTKAVGIPTIDLSESRARTSKSIVEAC+HFG FKVINHGVSDHAIANLER
Sbjct: 1   MPFPTATRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLER 60

Query: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
           QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILL SDPASIAETSASISNS
Sbjct: 61  QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASISNS 120

Query: 121 PLGFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180
           PL FSYVVNEYVK VRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV
Sbjct: 121 PLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAV 180

Query: 181 KHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240
           KHTEKSRELSSKSHLSTN IGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA
Sbjct: 181 KHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSA 240

Query: 241 FYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ 300
           FY+LIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMML KDQ
Sbjct: 241 FYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLPKDQ 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 332
           SLYKPFTWAQYKKAAYSLRLADSRL+LFKAS
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLHLFKAS 331

[Close]

BLAST of CmoCh08G004780 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 330.1 bits (845), Expect = 2.0e-90
Identity = 168/327 (51.38%), Postives = 214/327 (65.44%), Query Frame = 0


Query: 5   TAMRSKKTKAVGIPTIDLS-ESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAF 64
           T  ++  +     P ID S   R++ S+ IV+ACE  G FKVINHGV    I   E +  
Sbjct: 12  TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71

Query: 65  DFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASIS-NSPL 124
           +FF +   +K RAGPA+PFGYGCK IG NGD+G+LEY+LL ++P ++A+ S +IS + P 
Sbjct: 72  EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131

Query: 125 GFSYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKH 184
            FS   N+Y++ VR+LACEI+DL  E LW    S  S LIRDV+SDS+LR+N+YP A   
Sbjct: 132 KFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA--- 191

Query: 185 TEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFY 244
                     +     +IGFGEHSDPQILT+LRSNDVDGL+I   DGLW P+  DP+ F+
Sbjct: 192 --------PYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFF 251

Query: 245 VLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSL 304
           VL+GD  QA+TN RF SVRHR +AN +KK RMS MYF APPL A I+P   M+  ++   
Sbjct: 252 VLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRR 311

Query: 305 YKPFTWAQYKKAAYSLRLADSRLNLFK 330
           Y  FTW  YKKA YSLRL   RL  FK
Sbjct: 312 YNSFTWGDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of CmoCh08G004780 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 293.5 bits (750), Expect = 2.1e-79
Identity = 165/326 (50.61%), Postives = 218/326 (66.87%), Query Frame = 0


Query: 9   SKKTKAV--GIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFF 68
           S+K  AV   IP ID+S+ R++ S  IV+ACE  G FKVINHGV    I+ +E+++ +FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 69  GQTAPEKRRAGPA-APFGYGCKAIGPNGDIGDLEYILLR-SDPASIAETSASISNSPLGF 128
            + A EK+   P   PF YG + IG NGD G++EY+L   +DPA           S L F
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPA---------FRSQLSF 124

Query: 129 SYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTE 188
           S  VN Y++ V++LA EILDL  EGL +P  S FS LI  V SDSVLR+N+YP + +   
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 189 KSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVL 248
           ++  LS +S +S  ++GFGEH+DPQILT+LRSN V GLQ+S  DG+W  V PDPSAF V 
Sbjct: 185 EA-NLSDQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVN 244

Query: 249 IGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQ-SLY 308
           +GD  Q MTN RF+SVRHRA+    +++R+S  YF  PPL A I P S M++T +Q  LY
Sbjct: 245 VGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLY 304

Query: 309 KPFTWAQYKKAAYSLRLADSRLNLFK 330
           + FTW +YKK AYSLRL DSRL++F+
Sbjct: 305 QTFTWGEYKKRAYSLRLEDSRLDMFR 317

[Close]

BLAST of CmoCh08G004780 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 289.7 bits (740), Expect = 3.1e-78
Identity = 146/314 (46.50%), Postives = 210/314 (66.88%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRA 76
           IP ++L++  A+T   IV+ACE FG FKV+NHGV    +  LE++A  FFG     K RA
Sbjct: 31  IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRA 90

Query: 77  GPAAPFGYGCKAIGPNGDIGDLEYILLRSDP-ASIAETSASISNSPLGFSYVVNEYVKCV 136
           GP  P+GYG K IGPNGD+G +EY+LL ++P  S  +TSA    +P  F   V EY+K +
Sbjct: 91  GPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEI 150

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           +E++ ++L++V E L +  +   S ++RD +SDS LR+N+YPAA +  EK          
Sbjct: 151 KEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEAEK---------- 210

Query: 197 STNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTND 256
              K+GFGEH+DPQI+++LRSN+  GLQI + DG W  V PD S+F++ +GDA Q MTN 
Sbjct: 211 -MVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNG 270

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAA 316
           RF SV+HR +A +++++R+S++YFG PPL+  IAP   ++  +D  LYK FTW+QYK +A
Sbjct: 271 RFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSA 330

Query: 317 YSLRLADSRLNLFK 330
           Y  +L D RL LF+
Sbjct: 331 YKSKLGDYRLGLFE 330

[Close]

BLAST of CmoCh08G004780 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 278.9 bits (712), Expect = 5.4e-75
Identity = 147/320 (45.94%), Postives = 206/320 (64.38%), Query Frame = 0


Query: 11  KTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFFGQTA 70
           K + V IP IDL++S A+T   IV+ACE FG FKVINHGV    +  LE++A +FF    
Sbjct: 21  KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80

Query: 71  PEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPA-SIAETSASISNSPLGFSYVVN 130
             K +AGP  PFGYG K IGPNGD+G LEYILL ++      +T+A   ++P  F   V 
Sbjct: 81  SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVE 140

Query: 131 EYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSREL 190
           EY+K ++ ++ + L++V E L +  K   S L++  +SDS LR+N+YP   +   K    
Sbjct: 141 EYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVK---- 200

Query: 191 SSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAF 250
                    +IGFGEH+DPQ++++LRSND +GLQI + DG W  V PD S+F+VL+GD  
Sbjct: 201 --------EEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTL 260

Query: 251 QAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWA 310
           Q MTN RF SV+HR +  N+K++R+S++YF  PPL+  IAP S ++  +D  LY  FTW+
Sbjct: 261 QVMTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWS 320

Query: 311 QYKKAAYSLRLADSRLNLFK 330
           QYK +AY  +L D RL LF+
Sbjct: 321 QYKLSAYKTKLGDYRLGLFE 325

[Close]

BLAST of CmoCh08G004780 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 255.4 bits (651), Expect = 6.4e-68
Identity = 131/314 (41.72%), Postives = 198/314 (63.06%), Query Frame = 0


Query: 17  IPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLERQAFDFFGQTAPEKRRA 76
           IP ID+S+  ++   ++V+ACE FG FKVINHGVS   ++ LE +  DFF     EK + 
Sbjct: 18  IPVIDMSDPESK--HALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 77

Query: 77  GPAAPFGYGCKAIGPNGDIGDLEYILLRSD-PASIAETSASISNSPLGFSYVVNEYVKCV 136
               PFGYG   IG NGD+G +EY+L+ ++  +       S+  SP  F   + EY   V
Sbjct: 78  A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSV 137

Query: 137 RELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRINYYPAAVKHTEKSRELSSKSHL 196
           R++  ++L+ + +GL +  ++  S L+ D  +DS+LR+N+YP      +K+         
Sbjct: 138 RKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNG------- 197

Query: 197 STNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPVCPDPSAFYVLIGDAFQAMTND 256
             N IGFGEH+DPQI+++LRSN+  GLQI+L+DG W  V PD ++F+  +GD+ Q MTN 
Sbjct: 198 GKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNG 257

Query: 257 RFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDMMLTKDQSLYKPFTWAQYKKAA 316
           RF SVRHR +A N KK+R+S++YF  P L   IAP + ++  +D+ LY+ FTW++YK + 
Sbjct: 258 RFKSVRHRVLA-NCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNST 317

Query: 317 YSLRLADSRLNLFK 330
           Y+ RL+D+RL  F+
Sbjct: 318 YNSRLSDNRLQQFE 320

The following BLAST results are available for this feature:

BLAST of CmoCh08G004780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XHM5	6.2e-108	58.01	Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1	[more]
Q9FZ21	2.9e-89	51.38	Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ...	[more]
Q9C7Z1	3.0e-78	50.61	Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ...	[more]
Q9XFR9	4.3e-77	46.50	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9XG83	2.8e-76	46.37	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]

BLAST of CmoCh08G004780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8N0	9.6e-189	100.00	gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460...	[more]
A0A6J1KWQ4	7.9e-183	96.98	gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=...	[more]
A0A6J1GRC3	2.6e-154	83.99	gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456...	[more]
A0A6J1JXB5	4.2e-152	83.08	gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11148834...	[more]
A0A6J1DEJ9	1.5e-149	81.63	gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 ...	[more]

BLAST of CmoCh08G004780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022959634.1	2.0e-188	100.00	gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata]	[more]
KAG6593256.1	3.2e-186	99.09	Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia...	[more]
KAG7025608.1	4.2e-186	99.09	Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_023514766.1	1.6e-185	98.49	gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_023004594.1	1.6e-182	96.98	gibberellin 2-beta-dioxygenase 2 [Cucurbita maxima]	[more]

BLAST of CmoCh08G004780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02400.1	2.0e-90	51.38	gibberellin 2-oxidase 6	[more]
AT1G47990.1	2.1e-79	50.61	gibberellin 2-oxidase 4	[more]
AT1G30040.1	3.1e-78	46.50	gibberellin 2-oxidase	[more]
AT2G34555.1	5.4e-75	45.94	gibberellin 2-oxidase 3	[more]
AT1G78440.1	6.4e-68	41.72	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 33..50 score: 45.45 coord: 241..267 score: 27.27 coord: 205..226 score: 32.23
None	No IPR available	PANTHER	PTHR47990:SF45	GIBBERELLIN 2-BETA-DIOXYGENASE 6	coord: 4..329
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..329
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 10..321
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..319 e-value: 1.4E-67 score: 230.3
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 17..81 e-value: 5.5E-13 score: 49.7
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 168..280 e-value: 2.6E-21 score: 76.0
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 166..282 score: 12.229617

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh08G004780.1	CmoCh08G004780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding