CmoCh08G004310 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh08G004310 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 6.7e-200
Identity = 355/571 (62.17%), Postives = 432/571 (75.66%), Query Frame = 0

Query: 9   LILLTLYITT--AQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRKSLSSS 68
           LI L+ ++ +  A     + ++++C +TPDP YCKS  P+  GNV  YG  S RKSLS S
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 69  QKFLRLVKKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRTVNTTSSK-----LS 128
           +KF+R V +Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF TVN TS+K     LS
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 129 EMKADDVQSLLSAILTNQQTCLDGLKATA---GSWSVKNGLSLPLASDTKLYSMSLALFT 188
             KADD+Q+LLSA LTN+QTCL+GL   A    +W+V+ G++LPL +DTKL  +SLALFT
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFT 187

Query: 189 KGWVPKKKKRSTWTAAGRQGG----------FRNGRMSLKMSNRAKAIYEMATRRNLLET 248
           KGWVPKKKKR+ +  A  + G          FRNG + LKM+ + KA+YE  +RR L + 
Sbjct: 188 KGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADG 247

Query: 249 DDGGD----DQISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEE 308
           D  GD      + I DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+ G+YEE
Sbjct: 248 DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEE 307

Query: 309 YVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAG 368
           Y+SIAKNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNTAG
Sbjct: 308 YISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAG 367

Query: 369 AIKHQAVAVRNGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQ 428
             KHQAVA+R+GAD S FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVVFQ
Sbjct: 368 PEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQ 427

Query: 429 NCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPW 488
           NCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  ++   VKT+LGRPW
Sbjct: 428 NCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGRPW 487

Query: 489 KEYSRTVYLQSFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLI 548
           KEYSRTVY+QS++D  + P GWR W+ DFAL+T YYAE+ N GPGSNT+ RV W G+H+I
Sbjct: 488 KEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI 547

Query: 549 NDTDAGNFTAANFVLADDWLPQTGVPYTSGL 555
           N TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 548 NSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of CmoCh08G004310 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 679.5 bits (1752), Expect = 3.2e-194
Identity = 350/561 (62.39%), Postives = 434/561 (77.36%), Query Frame = 0

Query: 9   LILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQT-GNVYSYGRSSFRKSLSSSQ 68
           L L TL+I +  +  S +++++C +TPDP +CKS  P  + G+V  YGR S RKSL+ S+
Sbjct: 13  LSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSR 72

Query: 69  KFLRLVKKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVN-TTSSK-LSEMKA 128
           KF R + +YL R+ + L+  AV AL+DC+ LA L  DYL TSF TVN TTSSK LS  KA
Sbjct: 73  KFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKA 132

Query: 129 DDVQSLLSAILTNQQTCLDGLK-ATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPK 188
           D++Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL +DTKL+S+SLALFTKGWVPK
Sbjct: 133 DEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPK 192

Query: 189 KKK----------RSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDD 248
           KKK          ++T +       FRNG + LKM+   +A+YE  +RR L + DD   +
Sbjct: 193 KKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDVN 252

Query: 249 QISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRY 308
            + + DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V+ GVYEE V IAKNKRY
Sbjct: 253 TVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRY 312

Query: 309 LMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVR 368
           LMMIGDGIN+TV+TGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAVA+R
Sbjct: 313 LMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMR 372

Query: 369 NGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 428
           + ADLS FY CSFE YQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR PM
Sbjct: 373 SSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPM 432

Query: 429 ANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQ 488
            NQFNAITAQGRTDPNQNTG SIHNC I  ADDL  ++   VKT+LGRPWKEYSRTV++Q
Sbjct: 433 QNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFMQ 492

Query: 489 SFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTA 548
           S++DE++ P GWR W+ DFAL+T YYAE+ N G GS+T++RV W G+H+IN TDA NFT 
Sbjct: 493 SYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTV 552

Query: 549 ANFVLADDWLPQTGVPYTSGL 555
            NF+L D W+ Q+GVPY SGL
Sbjct: 553 ENFLLGDGWMVQSGVPYISGL 571

BLAST of CmoCh08G004310 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 642.9 bits (1657), Expect = 3.4e-183
Identity = 328/558 (58.78%), Postives = 413/558 (74.01%), Query Frame = 0

Query: 5   TFTSLILLTLYITTAQIPLSAAAAS---VCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRK 64
           T   L  L     +AQIP    A S   +C   PDPSYC+S LPNQ G++YSYGR S R+
Sbjct: 10  TLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRR 69

Query: 65  SLSSSQKFLRLVKKYLRSRSSLTVPA-VRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 124
           SLS +++F+ ++   L  +  +   + V ALEDC+ LA L +DYL +S +T ++T + LS
Sbjct: 70  SLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LS 129

Query: 125 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 184
             +A+DV + LSA +TN+QTCL+GLK+TA     +NGLS  L +DTKLY +SLALF+KGW
Sbjct: 130 LSRAEDVHTFLSAAITNEQTCLEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGW 189

Query: 185 VPKKKK-RSTWTAAGRQG---GFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQIS 244
           VP++++ R  W    R     GFRNG++ LKM+ RA+A+Y   TRR LL++D    D + 
Sbjct: 190 VPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQ 249

Query: 245 IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM 304
           + DIV V Q+G+GNFTTIN AIAAAPN +  ++GYFLI+V+ G+YEEYV + KNKRY+MM
Sbjct: 250 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 309

Query: 305 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 364
           IGDGINQTVITGNRSVVDGWTTFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G 
Sbjct: 310 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 369

Query: 365 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ 424
           DLS FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q
Sbjct: 370 DLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQ 429

Query: 425 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM 484
            N +TAQGRTDPNQNTGT+IH C I  ADDLA  +   VKT+LGRPWKEYSRTV +Q+++
Sbjct: 430 SNEVTAQGRTDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYI 489

Query: 485 DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF 544
           D  + P+GW AW  DFAL+T YYAE+ N GPGS+T+ RV W G+H+IN TDA NFT  NF
Sbjct: 490 DGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNF 549

Query: 545 VLADDWLPQTGVPYTSGL 555
           ++ + W+ QTGVP+  GL
Sbjct: 550 LVGEGWIGQTGVPFVGGL 558

BLAST of CmoCh08G004310 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.8e-136
Identity = 265/549 (48.27%), Postives = 352/549 (64.12%), Query Frame = 0

Query: 24  SAAAASVCTSTPDPSYCKSTL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR-- 83
           S + +  C STP P  C++ L    +   + Y YG+ + ++ L  + +  +++  Y R  
Sbjct: 76  SQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRV 135

Query: 84  --SRSSLTVPAVRALEDCQLLAGLNIDYL---TTSFRTVNTTSSKLSEMKADDVQSLLSA 143
                S T   + A+ DC  L+ L+++YL   TT  +T    ++ L E     V SLLS 
Sbjct: 136 ESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVE----HVNSLLSG 195

Query: 144 ILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAA 203
           ++TNQQTCLDGL      ++    +  P+ + T+LYS+SL L +   + +  KR    + 
Sbjct: 196 VVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFK-ASK 255

Query: 204 GRQGGFRNGRMSLKMSNRAKAIYEMA--------TRRNLLETDDGGDDQISIRDIVIVSQ 263
           G+  G  N      +    K + +          T RNL E  +     I +   VIV  
Sbjct: 256 GKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 315

Query: 264 DGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTV 323
             S NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+
Sbjct: 316 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 375

Query: 324 ITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCS 383
           ITGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY CS
Sbjct: 376 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 435

Query: 384 FEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGR 443
           FEGYQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PMA Q NAITA GR
Sbjct: 436 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 495

Query: 444 TDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGW 503
            DPNQNTG SI NC I AA DLA     A+ TFLGRPWK YSRTV++QS++ +++ P GW
Sbjct: 496 LDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGW 555

Query: 504 RAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQ 555
             W+    L+T YY E+ NFGPG+NT++RV WLG++L+N  +A NFT  NF + D WLPQ
Sbjct: 556 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 615

BLAST of CmoCh08G004310 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 4.9e-134
Identity = 258/539 (47.87%), Postives = 344/539 (63.82%), Query Frame = 0

Query: 31  CTSTPDPSYCK---STLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR----SRSSL 90
           C STP P  C+   S + +   + Y YG+ + ++ L  +++  +++ ++ +       + 
Sbjct: 88  CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEADPGTS 147

Query: 91  TVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLSEMKADDVQSLLSAILTNQQTCLD 150
           TV  V A+ DC  LA L+++YL T    +   +  ++    D V SLL  ++TNQQTCLD
Sbjct: 148 TVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSLLGGVVTNQQTCLD 207

Query: 151 GLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAAGRQGGFRNGR 210
           GL      ++    +  PL + T+LYS+SL L +       K+     + G+  G  N  
Sbjct: 208 GLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYK--GSKGKIFGGGNKP 267

Query: 211 MSLKMSNRAKAIYEMATR--------RNLLETDDGGDDQISIRDIVIVSQDGSGNFTTIN 270
           +   +    K + +   +        RNL E  +     I +R+ V V    + NF TI 
Sbjct: 268 VREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDNFPTIT 327

Query: 271 EAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDG 330
           EA+AAAPN++ P  GYF+I+   G+YEEYV I+  KR +M+IGDGIN+T+I+GN S +DG
Sbjct: 328 EAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSFIDG 387

Query: 331 WTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEGYQDTLYT 390
           WTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFEGYQDTLY 
Sbjct: 388 WTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYV 447

Query: 391 HSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGRTDPNQNTGTS 450
           HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PMANQ NA+TA GRTDPNQ TG S
Sbjct: 448 HSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTGIS 507

Query: 451 IHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGWRAWDKDFALN 510
           I NC I AA DLA +   +  TFLGRPWK YSRTVY+QS++ +++ P GW  W+    L+
Sbjct: 508 IINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTTGLD 567

Query: 511 TSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQTGVPYTSGL 555
           T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT +P+  GL
Sbjct: 568 TISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPFYGGL 620

BLAST of CmoCh08G004310 vs. ExPASy TrEMBL
Match: A0A6J1H5E4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460725 PE=3 SV=1)

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60
           MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120
           KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120

Query: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180
           EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240
           VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI
Sbjct: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240

Query: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300
           VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG
Sbjct: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300

Query: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360
           INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST
Sbjct: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360

Query: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420
           FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI
Sbjct: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420

Query: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480
           TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI
Sbjct: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480

Query: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540
           NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD
Sbjct: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540

Query: 541 DWLPQTGVPYTSGLTE 557
           DWLPQTGVPYTSGLTE
Sbjct: 541 DWLPQTGVPYTSGLTE 556

BLAST of CmoCh08G004310 vs. ExPASy TrEMBL
Match: A0A6J1KSC0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111497795 PE=3 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 4.1e-310
Identity = 545/556 (98.02%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60
           MAATTF+SLILL+LYITTAQIPLS  AASVCTSTPDPSYCKS LPNQTGNVYSYGRSSFR
Sbjct: 1   MAATTFSSLILLSLYITTAQIPLS--AASVCTSTPDPSYCKSALPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120
           KSLSSSQKFLRLVKKYLRSRSSLTVPAV ALEDC+LLAGLNIDYLTTSFR VNTTSSKL 
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLP 120

Query: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180
           EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240
           VPKKKKRSTWTAAG  GGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI
Sbjct: 181 VPKKKKRSTWTAAGSLGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240

Query: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300
           VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG
Sbjct: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300

Query: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360
           INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST
Sbjct: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360

Query: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420
           FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI
Sbjct: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420

Query: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480
           TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI
Sbjct: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480

Query: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540
           NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD
Sbjct: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540

Query: 541 DWLPQTGVPYTSGLTE 557
           DWLPQTGVPYTSGLTE
Sbjct: 541 DWLPQTGVPYTSGLTE 554

BLAST of CmoCh08G004310 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 6.4e-286
Identity = 494/552 (89.49%), Postives = 521/552 (94.38%), Query Frame = 0

Query: 6   FTSLILLTLYITTAQIPLSAA-AASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRKSLS 65
           F  L+L++LYIT A I  SAA   SVC+S PDPSYCKS LPNQTGNVYSYGRSS RKSLS
Sbjct: 11  FYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLS 70

Query: 66  SSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLSEMKA 125
           SSQKFLRLV+KYLRSRSSLTV AVR+LEDCQLLAGLNIDYLTTSFRTVN+TS  L EMKA
Sbjct: 71  SSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKA 130

Query: 126 DDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKK 185
           +DVQSLLSAILTNQQTCLDGLKATAGSWS+KNGLSLPL +DTKLYSMSLA FTKGWVPKK
Sbjct: 131 EDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLAFFTKGWVPKK 190

Query: 186 KKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDIVIVS 245
           KK+ TWT+AGRQGGF NGRMS KMS+R KAIYEMATRRNLL+TDDGGDDQIS+RDIV+VS
Sbjct: 191 KKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGGDDQISVRDIVVVS 250

Query: 246 QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQT 305
           QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV  GVYEEYV+IAKNKRYLMMIGDGINQT
Sbjct: 251 QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQT 310

Query: 306 VITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLC 365
           V+TGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN ADLSTFYLC
Sbjct: 311 VVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNSADLSTFYLC 370

Query: 366 SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQG 425
           SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPMANQFNAITAQG
Sbjct: 371 SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQG 430

Query: 426 RTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAG 485
           RTDPNQNTGTSIHNCRITAADDLANNTG  V TFLGRPWKEYSRTVY+QSFM++LINPAG
Sbjct: 431 RTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQSFMNDLINPAG 490

Query: 486 WRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLP 545
           WRAWD DFALNTSYYAEFGNFGPGSNTSERVNW+GFHLI+DTDAGNFTA NF++ADDW+P
Sbjct: 491 WRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNFLMADDWVP 550

Query: 546 QTGVPYTSGLTE 557
           QTGVPYTSGLTE
Sbjct: 551 QTGVPYTSGLTE 562

BLAST of CmoCh08G004310 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 8.4e-286
Identity = 494/552 (89.49%), Postives = 521/552 (94.38%), Query Frame = 0

Query: 6   FTSLILLTLYITTAQIPLSAA-AASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRKSLS 65
           F SL+L++LYIT A I  SAA   SVC+S PDPSYCKS LPNQTGNVYSYGRSS RKSLS
Sbjct: 11  FYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSYGRSSVRKSLS 70

Query: 66  SSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLSEMKA 125
           SSQKFLRLV+KYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFRTVN+TS  L EMKA
Sbjct: 71  SSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNSTSWTLPEMKA 130

Query: 126 DDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKK 185
           +DVQSLLSAILTNQQTCLDGLKATAGSWS+KNGLSLPLA+DTKLYSMSLA FTKGWVPKK
Sbjct: 131 EDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLAFFTKGWVPKK 190

Query: 186 KKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDIVIVS 245
           KK+ TWT+AGRQGGF NGRMSLKMS+R KAIYE ATRRNLL+T+DGGDDQIS+RDIV+VS
Sbjct: 191 KKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQISVRDIVVVS 250

Query: 246 QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQT 305
           QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFV  GVYEEYV+IAKNKRYLMMIGDGINQT
Sbjct: 251 QDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYLMMIGDGINQT 310

Query: 306 VITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLC 365
           V+TGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRN AGAIKHQAVAVRN ADLSTFYLC
Sbjct: 311 VVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVAVRNSADLSTFYLC 370

Query: 366 SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQG 425
           SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPMANQFNAITAQG
Sbjct: 371 SFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMANQFNAITAQG 430

Query: 426 RTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAG 485
           RTDPNQNTGTSIHNCRITAADDLANN+G  V TFLGRPWKEYSRTVY+QSFMD+LINPAG
Sbjct: 431 RTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQSFMDDLINPAG 490

Query: 486 WRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLP 545
           WRAWD DFALNT YYAEFGNFGPGSNTSERVNW+GFHLI+DTDAGNFTA NF++ADDW+P
Sbjct: 491 WRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAGNFLMADDWVP 550

Query: 546 QTGVPYTSGLTE 557
           QTGVPYTSGLTE
Sbjct: 551 QTGVPYTSGLTE 562

BLAST of CmoCh08G004310 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.7e-284
Identity = 494/556 (88.85%), Postives = 521/556 (93.71%), Query Frame = 0

Query: 3   ATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRKS 62
           A  F  LIL++LYITT+  P      SVC+STPDPSYCKS LPNQTGNVYSYGRSSFRKS
Sbjct: 7   AAAFYVLILISLYITTSASP----TGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRKS 66

Query: 63  LSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLSEM 122
           LSSSQKFLRLV+K+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+ VN TS  L+EM
Sbjct: 67  LSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTEM 126

Query: 123 KADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVP 182
           KADDVQSLLSAILTNQQTCLDG+KATAGSWS+KNGLS PLASDTKLYS+SLA FTKGWVP
Sbjct: 127 KADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWVP 186

Query: 183 KKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDD--GGDDQISIRDI 242
           KKKK+ TW AAGRQGGFRNGRMSLKMS+R +AIYE ATRRNLL+TDD  G DDQI++RDI
Sbjct: 187 KKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRDI 246

Query: 243 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 302
           V+VSQDG+GNFTTINEAIAAAPNNSAPTDGYFLIFVS GVYEEYVSIAKNKRYLMMIGDG
Sbjct: 247 VVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGDG 306

Query: 303 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 362
           INQT++TGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGADLST
Sbjct: 307 INQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLST 366

Query: 363 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 422
           FYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM+NQFNAI
Sbjct: 367 FYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAI 426

Query: 423 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 482
           TAQGRTDPNQNTGTSI+NCRITAADDLANN+G  VKTFLGRPWKEYSRTVY+QSFMD+LI
Sbjct: 427 TAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLI 486

Query: 483 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 542
           NPAGWRAWD DFALNTSYYAEFGNFGPGSNTSERVNW GFHLINDTDAGNFTA NFVLAD
Sbjct: 487 NPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLAD 546

Query: 543 DWLPQTGVPYTSGLTE 557
           DWLPQTGVPYTSGLTE
Sbjct: 547 DWLPQTGVPYTSGLTE 558

BLAST of CmoCh08G004310 vs. NCBI nr
Match: XP_022959747.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60
           MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120
           KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120

Query: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180
           EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240
           VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI
Sbjct: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240

Query: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300
           VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG
Sbjct: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300

Query: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360
           INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST
Sbjct: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360

Query: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420
           FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI
Sbjct: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420

Query: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480
           TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI
Sbjct: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480

Query: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540
           NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD
Sbjct: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540

Query: 541 DWLPQTGVPYTSGLTE 557
           DWLPQTGVPYTSGLTE
Sbjct: 541 DWLPQTGVPYTSGLTE 556

BLAST of CmoCh08G004310 vs. NCBI nr
Match: KAG7030208.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 554/558 (99.28%), Postives = 555/558 (99.46%), Query Frame = 0

Query: 1   MAATTFTSLILLTLYITTAQIPLSA--AAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSS 60
           MAATTFTSLILLTLYITTAQIPLSA  A+ASVCTSTPDPSYCKSTLPNQTGNVYSYGRSS
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAASASASVCTSTPDPSYCKSTLPNQTGNVYSYGRSS 60

Query: 61  FRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSK 120
           FRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSK
Sbjct: 61  FRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSK 120

Query: 121 LSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTK 180
           LSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTK
Sbjct: 121 LSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTK 180

Query: 181 GWVPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIR 240
           GWVPKKKKRSTWTAAG QGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIR
Sbjct: 181 GWVPKKKKRSTWTAAGSQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIR 240

Query: 241 DIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIG 300
           DIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIG
Sbjct: 241 DIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIG 300

Query: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL 360
           DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL
Sbjct: 301 DGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADL 360

Query: 361 STFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFN 420
           STFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFN
Sbjct: 361 STFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFN 420

Query: 421 AITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDE 480
           AITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDE
Sbjct: 421 AITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDE 480

Query: 481 LINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVL 540
           LINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVL
Sbjct: 481 LINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVL 540

Query: 541 ADDWLPQTGVPYTSGLTE 557
           ADDWLPQTGVPYTSGLTE
Sbjct: 541 ADDWLPQTGVPYTSGLTE 558

BLAST of CmoCh08G004310 vs. NCBI nr
Match: KAG6593215.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 554/560 (98.93%), Postives = 555/560 (99.11%), Query Frame = 0

Query: 1   MAATTFTSLILLTLYITTAQIPLSA----AAASVCTSTPDPSYCKSTLPNQTGNVYSYGR 60
           MAATTFTSLILLTLYITTAQIPLSA    A+ASVCTSTPDPSYCKSTLPNQTGNVYSYGR
Sbjct: 1   MAATTFTSLILLTLYITTAQIPLSAASASASASVCTSTPDPSYCKSTLPNQTGNVYSYGR 60

Query: 61  SSFRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTS 120
           SSFRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTS
Sbjct: 61  SSFRKSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTS 120

Query: 121 SKLSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALF 180
           SKLSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALF
Sbjct: 121 SKLSEMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALF 180

Query: 181 TKGWVPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQIS 240
           TKGWVPKKKKRSTWTAAG QGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQIS
Sbjct: 181 TKGWVPKKKKRSTWTAAGSQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQIS 240

Query: 241 IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM 300
           IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM
Sbjct: 241 IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM 300

Query: 301 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 301 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ 420
           DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ
Sbjct: 361 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM 480
           FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM 480

Query: 481 DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF 540
           DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF
Sbjct: 481 DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF 540

Query: 541 VLADDWLPQTGVPYTSGLTE 557
           VLADDWLPQTGVPYTSGLTE
Sbjct: 541 VLADDWLPQTGVPYTSGLTE 560

BLAST of CmoCh08G004310 vs. NCBI nr
Match: XP_023513640.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 546/556 (98.20%), Postives = 553/556 (99.46%), Query Frame = 0

Query: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60
           MAATTF+SLILL+LYITTAQIPLSAA+ASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR
Sbjct: 1   MAATTFSSLILLSLYITTAQIPLSAASASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120
           KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120

Query: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180
           EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGL+LPLASDTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLALPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240
           VPKKKKRSTWTAAG QGGFRNGRMSLKMS+RAKAIYEMATRRNLL+T+ GGDDQISIRDI
Sbjct: 181 VPKKKKRSTWTAAGSQGGFRNGRMSLKMSSRAKAIYEMATRRNLLQTEGGGDDQISIRDI 240

Query: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300
           VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG
Sbjct: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300

Query: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360
           INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST
Sbjct: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360

Query: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420
           FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI
Sbjct: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420

Query: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480
           TAQGRTDPNQNTGTSIHNCRITAADDLANNTGV VKTFLGRPWKEYSRTVYLQSFMDELI
Sbjct: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVTVKTFLGRPWKEYSRTVYLQSFMDELI 480

Query: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540
           NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD
Sbjct: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540

Query: 541 DWLPQTGVPYTSGLTE 557
           DWLPQTGVPYTSGLTE
Sbjct: 541 DWLPQTGVPYTSGLTE 556

BLAST of CmoCh08G004310 vs. NCBI nr
Match: XP_023004516.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita maxima])

HSP 1 Score: 1073.2 bits (2774), Expect = 8.5e-310
Identity = 545/556 (98.02%), Postives = 548/556 (98.56%), Query Frame = 0

Query: 1   MAATTFTSLILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFR 60
           MAATTF+SLILL+LYITTAQIPLS  AASVCTSTPDPSYCKS LPNQTGNVYSYGRSSFR
Sbjct: 1   MAATTFSSLILLSLYITTAQIPLS--AASVCTSTPDPSYCKSALPNQTGNVYSYGRSSFR 60

Query: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 120
           KSLSSSQKFLRLVKKYLRSRSSLTVPAV ALEDC+LLAGLNIDYLTTSFR VNTTSSKL 
Sbjct: 61  KSLSSSQKFLRLVKKYLRSRSSLTVPAVGALEDCKLLAGLNIDYLTTSFRAVNTTSSKLP 120

Query: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180
           EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW
Sbjct: 121 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 180

Query: 181 VPKKKKRSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240
           VPKKKKRSTWTAAG  GGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI
Sbjct: 181 VPKKKKRSTWTAAGSLGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQISIRDI 240

Query: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300
           VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG
Sbjct: 241 VIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDG 300

Query: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360
           INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST
Sbjct: 301 INQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLST 360

Query: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420
           FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI
Sbjct: 361 FYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAI 420

Query: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480
           TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI
Sbjct: 421 TAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELI 480

Query: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540
           NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD
Sbjct: 481 NPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLAD 540

Query: 541 DWLPQTGVPYTSGLTE 557
           DWLPQTGVPYTSGLTE
Sbjct: 541 DWLPQTGVPYTSGLTE 554

BLAST of CmoCh08G004310 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 698.4 bits (1801), Expect = 4.8e-201
Identity = 355/571 (62.17%), Postives = 432/571 (75.66%), Query Frame = 0

Query: 9   LILLTLYITT--AQIPLSAAAASVCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRKSLSSS 68
           LI L+ ++ +  A     + ++++C +TPDP YCKS  P+  GNV  YG  S RKSLS S
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 69  QKFLRLVKKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRTVNTTSSK-----LS 128
           +KF+R V +Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF TVN TS+K     LS
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 129 EMKADDVQSLLSAILTNQQTCLDGLKATA---GSWSVKNGLSLPLASDTKLYSMSLALFT 188
             KADD+Q+LLSA LTN+QTCL+GL   A    +W+V+ G++LPL +DTKL  +SLALFT
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFT 187

Query: 189 KGWVPKKKKRSTWTAAGRQGG----------FRNGRMSLKMSNRAKAIYEMATRRNLLET 248
           KGWVPKKKKR+ +  A  + G          FRNG + LKM+ + KA+YE  +RR L + 
Sbjct: 188 KGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADG 247

Query: 249 DDGGD----DQISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEE 308
           D  GD      + I DIV VSQDG+GNFT I  A+AAAPNN+  + G+FLI+V+ G+YEE
Sbjct: 248 DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEE 307

Query: 309 YVSIAKNKRYLMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAG 368
           Y+SIAKNKRY+MMIGDGINQTV+TGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNTAG
Sbjct: 308 YISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAG 367

Query: 369 AIKHQAVAVRNGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQ 428
             KHQAVA+R+GAD S FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVVFQ
Sbjct: 368 PEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQ 427

Query: 429 NCNIYPRLPMANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPW 488
           NCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  ++   VKT+LGRPW
Sbjct: 428 NCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGRPW 487

Query: 489 KEYSRTVYLQSFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLI 548
           KEYSRTVY+QS++D  + P GWR W+ DFAL+T YYAE+ N GPGSNT+ RV W G+H+I
Sbjct: 488 KEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI 547

Query: 549 NDTDAGNFTAANFVLADDWLPQTGVPYTSGL 555
           N TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 548 NSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of CmoCh08G004310 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 679.5 bits (1752), Expect = 2.3e-195
Identity = 350/561 (62.39%), Postives = 434/561 (77.36%), Query Frame = 0

Query: 9   LILLTLYITTAQIPLSAAAASVCTSTPDPSYCKSTLPNQT-GNVYSYGRSSFRKSLSSSQ 68
           L L TL+I +  +  S +++++C +TPDP +CKS  P  + G+V  YGR S RKSL+ S+
Sbjct: 13  LSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQSR 72

Query: 69  KFLRLVKKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRTVN-TTSSK-LSEMKA 128
           KF R + +YL R+ + L+  AV AL+DC+ LA L  DYL TSF TVN TTSSK LS  KA
Sbjct: 73  KFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSKA 132

Query: 129 DDVQSLLSAILTNQQTCLDGLK-ATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPK 188
           D++Q+LLSA LTN+QTCLDG+  A + SW+++NG++LPL +DTKL+S+SLALFTKGWVPK
Sbjct: 133 DEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVPK 192

Query: 189 KKK----------RSTWTAAGRQGGFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDD 248
           KKK          ++T +       FRNG + LKM+   +A+YE  +RR L + DD   +
Sbjct: 193 KKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDVN 252

Query: 249 QISIRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRY 308
            + + DIV V+Q+G+GNFTTI EA+ +APN +  T GYF+I+V+ GVYEE V IAKNKRY
Sbjct: 253 TVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRY 312

Query: 309 LMMIGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVR 368
           LMMIGDGIN+TV+TGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAVA+R
Sbjct: 313 LMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMR 372

Query: 369 NGADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPM 428
           + ADLS FY CSFE YQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR PM
Sbjct: 373 SSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPM 432

Query: 429 ANQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQ 488
            NQFNAITAQGRTDPNQNTG SIHNC I  ADDL  ++   VKT+LGRPWKEYSRTV++Q
Sbjct: 433 QNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFMQ 492

Query: 489 SFMDELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTA 548
           S++DE++ P GWR W+ DFAL+T YYAE+ N G GS+T++RV W G+H+IN TDA NFT 
Sbjct: 493 SYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTV 552

Query: 549 ANFVLADDWLPQTGVPYTSGL 555
            NF+L D W+ Q+GVPY SGL
Sbjct: 553 ENFLLGDGWMVQSGVPYISGL 571

BLAST of CmoCh08G004310 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 642.9 bits (1657), Expect = 2.4e-184
Identity = 328/558 (58.78%), Postives = 413/558 (74.01%), Query Frame = 0

Query: 5   TFTSLILLTLYITTAQIPLSAAAAS---VCTSTPDPSYCKSTLPNQTGNVYSYGRSSFRK 64
           T   L  L     +AQIP    A S   +C   PDPSYC+S LPNQ G++YSYGR S R+
Sbjct: 10  TLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRR 69

Query: 65  SLSSSQKFLRLVKKYLRSRSSLTVPA-VRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLS 124
           SLS +++F+ ++   L  +  +   + V ALEDC+ LA L +DYL +S +T ++T + LS
Sbjct: 70  SLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKT-LS 129

Query: 125 EMKADDVQSLLSAILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGW 184
             +A+DV + LSA +TN+QTCL+GLK+TA     +NGLS  L +DTKLY +SLALF+KGW
Sbjct: 130 LSRAEDVHTFLSAAITNEQTCLEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGW 189

Query: 185 VPKKKK-RSTWTAAGRQG---GFRNGRMSLKMSNRAKAIYEMATRRNLLETDDGGDDQIS 244
           VP++++ R  W    R     GFRNG++ LKM+ RA+A+Y   TRR LL++D    D + 
Sbjct: 190 VPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQ 249

Query: 245 IRDIVIVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMM 304
           + DIV V Q+G+GNFTTIN AIAAAPN +  ++GYFLI+V+ G+YEEYV + KNKRY+MM
Sbjct: 250 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 309

Query: 305 IGDGINQTVITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 364
           IGDGINQTVITGNRSVVDGWTTFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G 
Sbjct: 310 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 369

Query: 365 DLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQ 424
           DLS FY CSFE YQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q
Sbjct: 370 DLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQ 429

Query: 425 FNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFM 484
            N +TAQGRTDPNQNTGT+IH C I  ADDLA  +   VKT+LGRPWKEYSRTV +Q+++
Sbjct: 430 SNEVTAQGRTDPNQNTGTAIHGCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYI 489

Query: 485 DELINPAGWRAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANF 544
           D  + P+GW AW  DFAL+T YYAE+ N GPGS+T+ RV W G+H+IN TDA NFT  NF
Sbjct: 490 DGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNF 549

Query: 545 VLADDWLPQTGVPYTSGL 555
           ++ + W+ QTGVP+  GL
Sbjct: 550 LVGEGWIGQTGVPFVGGL 558

BLAST of CmoCh08G004310 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 487.6 bits (1254), Expect = 1.3e-137
Identity = 265/549 (48.27%), Postives = 352/549 (64.12%), Query Frame = 0

Query: 24  SAAAASVCTSTPDPSYCKSTL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR-- 83
           S + +  C STP P  C++ L    +   + Y YG+ + ++ L  + +  +++  Y R  
Sbjct: 76  SQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRV 135

Query: 84  --SRSSLTVPAVRALEDCQLLAGLNIDYL---TTSFRTVNTTSSKLSEMKADDVQSLLSA 143
                S T   + A+ DC  L+ L+++YL   TT  +T    ++ L E     V SLLS 
Sbjct: 136 ESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVE----HVNSLLSG 195

Query: 144 ILTNQQTCLDGLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAA 203
           ++TNQQTCLDGL      ++    +  P+ + T+LYS+SL L +   + +  KR    + 
Sbjct: 196 VVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHA-LNRNLKRFK-ASK 255

Query: 204 GRQGGFRNGRMSLKMSNRAKAIYEMA--------TRRNLLETDDGGDDQISIRDIVIVSQ 263
           G+  G  N      +    K + +          T RNL E  +     I +   VIV  
Sbjct: 256 GKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 315

Query: 264 DGSGNFTTINEAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTV 323
             S NFTTI +AIAAAPNN+ P DGYF+I+   GVYEEY+ +  NK+ LM++GDGIN+T+
Sbjct: 316 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 375

Query: 324 ITGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCS 383
           ITGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+FY CS
Sbjct: 376 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 435

Query: 384 FEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGR 443
           FEGYQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PMA Q NAITA GR
Sbjct: 436 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 495

Query: 444 TDPNQNTGTSIHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGW 503
            DPNQNTG SI NC I AA DLA     A+ TFLGRPWK YSRTV++QS++ +++ P GW
Sbjct: 496 LDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQPVGW 555

Query: 504 RAWDKDFALNTSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQ 555
             W+    L+T YY E+ NFGPG+NT++RV WLG++L+N  +A NFT  NF + D WLPQ
Sbjct: 556 LEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQ 615

BLAST of CmoCh08G004310 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 479.6 bits (1233), Expect = 3.5e-135
Identity = 258/539 (47.87%), Postives = 344/539 (63.82%), Query Frame = 0

Query: 31  CTSTPDPSYCK---STLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVKKYLR----SRSSL 90
           C STP P  C+   S + +   + Y YG+ + ++ L  +++  +++ ++ +       + 
Sbjct: 88  CKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEADPGTS 147

Query: 91  TVPAVRALEDCQLLAGLNIDYLTTSFRTVNTTSSKLSEMKADDVQSLLSAILTNQQTCLD 150
           TV  V A+ DC  LA L+++YL T    +   +  ++    D V SLL  ++TNQQTCLD
Sbjct: 148 TVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSLLGGVVTNQQTCLD 207

Query: 151 GLKATAGSWSVKNGLSLPLASDTKLYSMSLALFTKGWVPKKKKRSTWTAAGRQGGFRNGR 210
           GL      ++    +  PL + T+LYS+SL L +       K+     + G+  G  N  
Sbjct: 208 GLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYK--GSKGKIFGGGNKP 267

Query: 211 MSLKMSNRAKAIYEMATR--------RNLLETDDGGDDQISIRDIVIVSQDGSGNFTTIN 270
           +   +    K + +   +        RNL E  +     I +R+ V V    + NF TI 
Sbjct: 268 VREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYETDNFPTIT 327

Query: 271 EAIAAAPNNSAPTDGYFLIFVSVGVYEEYVSIAKNKRYLMMIGDGINQTVITGNRSVVDG 330
           EA+AAAPN++ P  GYF+I+   G+YEEYV I+  KR +M+IGDGIN+T+I+GN S +DG
Sbjct: 328 EAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIISGNHSFIDG 387

Query: 331 WTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEGYQDTLYT 390
           WTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFEGYQDTLY 
Sbjct: 388 WTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFEGYQDTLYV 447

Query: 391 HSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMANQFNAITAQGRTDPNQNTGTS 450
           HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PMANQ NA+TA GRTDPNQ TG S
Sbjct: 448 HSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTDPNQKTGIS 507

Query: 451 IHNCRITAADDLANNTGVAVKTFLGRPWKEYSRTVYLQSFMDELINPAGWRAWDKDFALN 510
           I NC I AA DLA +   +  TFLGRPWK YSRTVY+QS++ +++ P GW  W+    L+
Sbjct: 508 IINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEWNGTTGLD 567

Query: 511 TSYYAEFGNFGPGSNTSERVNWLGFHLINDTDAGNFTAANFVLADDWLPQTGVPYTSGL 555
           T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT +P+  GL
Sbjct: 568 TISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDIPFYGGL 620