CmoCh07G008650 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh07G008650
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionIAA-amino acid hydrolase ILR1
LocationCmo_Chr07: 4086863 .. 4089489 (+)
RNA-Seq ExpressionCmoCh07G008650
SyntenyCmoCh07G008650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGCGGCGGCGGCGGCGGCATGGAGTTCAAGTTGTTTTGTTGGATGCTTGTTTTTGGAGTGGCGTTGTGGCGGCCGTCGGCGTCGGAGGTGGAGCACGGTGGAGATGGGTTGGAGCTACACTCACTGACACGGAAGTTATTGGGTTTGGCAAGGGAAGGGGAGTTCCTGGAGTGGATAAAAGGAGTTAGAAGGAGAATTCATGAGTATCCAGAGGTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTTGCTCGGCATCAGTTACCAATGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTAAAGGGGCTTCTTCTTCTTCTTCTTCTTCTTCATCTAGACCTGTTTTTGCTCTTAGAGCAGACATGGATGCCCTTCCTCTTCAGGTACTCTCTCTATAAACAAAGATGAAACCAGAGAATGGCATACAGATCAAATACAATGGAAGAAAGTAATTTGTCAAAAGTAACTTGGGAAAATGTAGAGCAAGCAAAAGACATTTGACTCTAAAAAGATTTATATTTTGCTACTAAAACTGAGATAGGCTTCATACATATCCATATCAGAATAGCATATCAAGAAACTGCTTCAACTAACCTTATTGCTTTCTATCCTTGCTCTAAGGTCTGATAATCTGTGAGATTCCACATTGATTGGAGAGGAGAACGAAACATTCTTTATAAAGACGTGGAAATCTCTCCCTAATAGACGCCGTATTAAAAACCTTGAAAGAAAGCCTGGAAGGAAAACCCCAAAGAGGACCATATCGGTTAGTGATGGGTTTGGGCTATTACAAATGGTATCAGAGCCAGACACCGAATGATGTGCCAGCAAGGACGCTGGACCTCAAAGGGGGTGGATTGTGAGATCCCAAATCGGTTGGAGAAGGGAACAAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAATAGATACGTTTTAAAAGCCTTGAGGGGAAGCCCGAAAGAAAAAATCCAAAGAGGACAATATCTACTAGCGGTAGGCTTGTGCTGGTATTTACCTTTTGCAAAACTAAAATTCAACCCTGCAAATTGTTGTCATCAATAGAGAAGATCATGCCACGGTAGAATAGGAGTGCAAATTGATGAATAGTTCAGATTCTCCAAGCTAAATATGTCTCATATAATTTTGAAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGCCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTATTACAGAGTAAAATAGAGAAACTGAAGGTAAAAATTTTCATACTTAGAATTGTAAATCCTATTTGTGCGATTCTGTTACCTGGGAAGGGGACTGACCAGGATTACTTGTATATGTTGTTATTTGAAGGGAACTGTGAAGTTAGTTTTCCAGCCAGGAGAAGAGTGTAATGGAGCTTATCAATTGCTACAAGAAGATGCCCTTGACGATATAGATGGAATCTTTGCATTGCACGTCTTGCCCACCTTACCAACCGGTGTCATAGCCTCGAGGCCTGGTCCAGTATGTGCGGGAGCAGGACACTTTTCGGCCTTGATACGAGGAAAGGGTGGACATGCTGCAGCCCCACATAAGACGAAGGACCCAGTTCTAGCAACAGCTTTTATAATTCAAGCTCTTCAACAAATTGTATCTCGTGAAACCGATCCTCTCGAGGCTGGAGTAAGTTTTGCGCCTTCTAAACGAAGTCCCTAATCTTCTCCGTTTGTTAGGATCATAAGAACAGAATAACATGAGAAAATTATTAACTGCTCATCAAGATTCAACACTTAAAGGTAATGCATAGCAAGGAAACTTGCAAACATGGTCGTAGTTAACTAATACTCAGCAATGTAGGTGGTGACTGTTGCATTTGTTGATGGAGGGCAAGCAGATAATGTCGTTCCCGAAACCGTGAAAGTTGGAGGAACTTTTAGGAGCTTGTCTCCTAAGGGTCTCTCTTACCTTAAGGAAAGGATCAGAGAGGTATAACATGCATTACACTTAATGAACTTAAACAAATTCCTATATTACTTTTGAAGCTGGGAAAAACTTAATTTCTTTTCAACAGGTCATCAACACACAAGCAGTAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATGTAGCATTGTTTGAGCATGCAAATAGGGTAGGAAATAGCTTACTTGGAGAATCCAATGTGCAACTCCTTCCATGGACCATGGGAGCAGAAGACTTTGGCTTCTTGTCACAGAAGATTGCAGCCACCATATACGGGATTGGGATAAGGAATGATACTCTGAGATCAAACCGGCCACTGCACTCACCGCACTTTGTCCTTGATGAGGAGGCCCTTCCAATTGGAGCAGCTCTTCATGCTGCTGTGGCTATGTCTTACTTGGAACACAACTGAGTTGTCAGTGATTAACTTCAATTTCAGTTCTGTAAGTAAAATAATATGTGAGGAACAAAACATGCCATAGGTATGCACATATTTTGCCAAAATAAAGAGAATTACACTTCATTACTACTGAAAATTTAGGGAGCACTTATTTGGTCATTCTTTCTAGAACAGTACAAC

mRNA sequence

ATGGCAGGCGGCGGCGGCGGCGGCATGGAGTTCAAGTTGTTTTGTTGGATGCTTGTTTTTGGAGTGGCGTTGTGGCGGCCGTCGGCGTCGGAGGTGGAGCACGGTGGAGATGGGTTGGAGCTACACTCACTGACACGGAAGTTATTGGGTTTGGCAAGGGAAGGGGAGTTCCTGGAGTGGATAAAAGGAGTTAGAAGGAGAATTCATGAGTATCCAGAGGTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTTGCTCGGCATCAGTTACCAATGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTAAAGGGGCTTCTTCTTCTTCTTCTTCTTCTTCATCTAGACCTGTTTTTGCTCTTAGAGCAGACATGGATGCCCTTCCTCTTCAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGCCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTATTACAGAGTAAAATAGAGAAACTGAAGGGAACTGTGAAGTTAGTTTTCCAGCCAGGAGAAGAGTGTAATGGAGCTTATCAATTGCTACAAGAAGATGCCCTTGACGATATAGATGGAATCTTTGCATTGCACGTCTTGCCCACCTTACCAACCGGTGTCATAGCCTCGAGGCCTGGTCCAGTATGTGCGGGAGCAGGACACTTTTCGGCCTTGATACGAGGAAAGGGTGGACATGCTGCAGCCCCACATAAGACGAAGGACCCAGTTCTAGCAACAGCTTTTATAATTCAAGCTCTTCAACAAATTGTATCTCGTGAAACCGATCCTCTCGAGGCTGGAGTGGTGACTGTTGCATTTGTTGATGGAGGGCAAGCAGATAATGTCGTTCCCGAAACCGTGAAAGTTGGAGGAACTTTTAGGAGCTTGTCTCCTAAGGGTCTCTCTTACCTTAAGGAAAGGATCAGAGAGGTCATCAACACACAAGCAGTAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATGTAGCATTGTTTGAGCATGCAAATAGGGTAGGAAATAGCTTACTTGGAGAATCCAATGTGCAACTCCTTCCATGGACCATGGGAGCAGAAGACTTTGGCTTCTTGTCACAGAAGATTGCAGCCACCATATACGGGATTGGGATAAGGAATGATACTCTGAGATCAAACCGGCCACTGCACTCACCGCACTTTGTCCTTGATGAGGAGGCCCTTCCAATTGGAGCAGCTCTTCATGCTGCTGTGGCTATGTCTTACTTGGAACACAACTGAGTTGTCAGTGATTAACTTCAATTTCAGTTCTGTAAGTAAAATAATATGTGAGGAACAAAACATGCCATAGGTATGCACATATTTTGCCAAAATAAAGAGAATTACACTTCATTACTACTGAAAATTTAGGGAGCACTTATTTGGTCATTCTTTCTAGAACAGTACAAC

Coding sequence (CDS)

ATGGCAGGCGGCGGCGGCGGCGGCATGGAGTTCAAGTTGTTTTGTTGGATGCTTGTTTTTGGAGTGGCGTTGTGGCGGCCGTCGGCGTCGGAGGTGGAGCACGGTGGAGATGGGTTGGAGCTACACTCACTGACACGGAAGTTATTGGGTTTGGCAAGGGAAGGGGAGTTCCTGGAGTGGATAAAAGGAGTTAGAAGGAGAATTCATGAGTATCCAGAGGTGGGATTTGAAGAGTACAGAACGAGTCAACTCGTGAGGTCTGAGCTCGACTTGCTCGGCATCAGTTACCAATGGCCTGTTGCCAAGACTGGAGTGGTGGCTTCCATTAAAGGGGCTTCTTCTTCTTCTTCTTCTTCTTCATCTAGACCTGTTTTTGCTCTTAGAGCAGACATGGATGCCCTTCCTCTTCAGGAACTGGTAGAATGGGAATTCAAAAGCAAGGTAGAAGGTAAAATGCATGCCTGTGGCCATGATTCACATGTTGCTATGCTATTAGGAGCAGCAAGGTTATTACAGAGTAAAATAGAGAAACTGAAGGGAACTGTGAAGTTAGTTTTCCAGCCAGGAGAAGAGTGTAATGGAGCTTATCAATTGCTACAAGAAGATGCCCTTGACGATATAGATGGAATCTTTGCATTGCACGTCTTGCCCACCTTACCAACCGGTGTCATAGCCTCGAGGCCTGGTCCAGTATGTGCGGGAGCAGGACACTTTTCGGCCTTGATACGAGGAAAGGGTGGACATGCTGCAGCCCCACATAAGACGAAGGACCCAGTTCTAGCAACAGCTTTTATAATTCAAGCTCTTCAACAAATTGTATCTCGTGAAACCGATCCTCTCGAGGCTGGAGTGGTGACTGTTGCATTTGTTGATGGAGGGCAAGCAGATAATGTCGTTCCCGAAACCGTGAAAGTTGGAGGAACTTTTAGGAGCTTGTCTCCTAAGGGTCTCTCTTACCTTAAGGAAAGGATCAGAGAGGTCATCAACACACAAGCAGTAGTGCACCATTGTTATGCTTCAGTGGAGTTTATGGAGGATACACCAGTAATGGTTAACAATGTAGCATTGTTTGAGCATGCAAATAGGGTAGGAAATAGCTTACTTGGAGAATCCAATGTGCAACTCCTTCCATGGACCATGGGAGCAGAAGACTTTGGCTTCTTGTCACAGAAGATTGCAGCCACCATATACGGGATTGGGATAAGGAATGATACTCTGAGATCAAACCGGCCACTGCACTCACCGCACTTTGTCCTTGATGAGGAGGCCCTTCCAATTGGAGCAGCTCTTCATGCTGCTGTGGCTATGTCTTACTTGGAACACAACTGA

Protein sequence

MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN
Homology
BLAST of CmoCh07G008650 vs. ExPASy Swiss-Prot
Match: P54968 (IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2)

HSP 1 Score: 488.4 bits (1256), Expect = 8.4e-137
Identity = 242/432 (56.02%), Postives = 317/432 (73.38%), Query Frame = 0

Query: 16  WMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVG 75
           + ++F    + P  S       G  L SL R +L  A++ EF EW++G+RR+IHE PE G
Sbjct: 7   FFVIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKIHENPETG 66

Query: 76  FEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVFALRADMDALP 135
           F+E++TSQLVR ELD LG+ Y++PVAKTGVVA I         S S+PVF LRADMDALP
Sbjct: 67  FQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWI--------GSCSKPVFGLRADMDALP 126

Query: 136 LQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEE-CNG 195
           LQELVEWE KSKV+GKMHACGHD+HVAMLLGAA+LLQ+    +KGTVKLVFQPGEE   G
Sbjct: 127 LQELVEWESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEGYAG 186

Query: 196 AYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHK 255
           AY++L+++ LDD+DGI ++HV P++P+G I SRPG V AGAG F+  + G+G HAA PH 
Sbjct: 187 AYEMLKDEILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAATPHF 246

Query: 256 TKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSP 315
           +KDPVLA +  + ALQQIVSRE DPLEAGVVTV +++GG A NV+P++ K GGTFRSLS 
Sbjct: 247 SKDPVLAASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRSLSN 306

Query: 316 KGLSYLKERIREVINTQAVVHHCYASVEFMEDT----PVMVNNVALFEHANRVGNSLLGE 375
            GL +++ RI+E+   QA V+ C A V F E      PVM N+  L+EH  +V  +++G+
Sbjct: 307 DGLLFIQRRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAMIGK 366

Query: 376 SNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAAL 435
           +N    P TMG EDF F +QK  A I+ +G++N+TL + +PLHSP+F +DEEALP+GAAL
Sbjct: 367 NNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAAL 426

Query: 436 HAAVAMSYLEHN 443
           HAA+A+SYL+ +
Sbjct: 427 HAAMAVSYLDEH 430

BLAST of CmoCh07G008650 vs. ExPASy Swiss-Prot
Match: Q851L5 (IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL3 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 5.3e-131
Identity = 236/410 (57.56%), Postives = 301/410 (73.41%), Query Frame = 0

Query: 43  SLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAK 102
           +L R+LL  AR  EF  W++G+RRRIH++PE+ F+E+RTS LVR+ELD LG++Y WPVA+
Sbjct: 8   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPVAQ 67

Query: 103 TGVVASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVA 162
           TGVVA++ GA+         PVF LRADMDALP+QE+VEWEFKS  +GKMHACGHD HVA
Sbjct: 68  TGVVATVVGAAGPG------PVFGLRADMDALPIQEMVEWEFKSLEDGKMHACGHDVHVA 127

Query: 163 MLLGAARLLQSKIEKLKGTVKLVFQPGEE-CNGAYQLLQEDALDDIDGIFALHVLPTLPT 222
           MLLGAA+LLQS+ +   G VKLVFQP EE   G Y +L+E A+DD+ GIF +HV   LP 
Sbjct: 128 MLLGAAKLLQSRRDHFNGKVKLVFQPAEEGYAGGYYVLEEGAVDDVQGIFGMHVDAGLPA 187

Query: 223 GVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLE 282
           GV+ASRPGP  AG+  F+A I GKGGHAAAPH   DP++A +  + +LQQIV+RETDPL+
Sbjct: 188 GVVASRPGPFLAGSARFTATINGKGGHAAAPHHAVDPIVAVSSAVLSLQQIVARETDPLQ 247

Query: 283 AGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASV 342
             VV+V  + GG+A NV+PE+V +GGT RS++  G+SYL +RIREVI  QA V+ C A+V
Sbjct: 248 GAVVSVTTIKGGEAFNVIPESVTLGGTLRSMTTDGMSYLMKRIREVIEGQAAVNRCTAAV 307

Query: 343 EFMEDT----PVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIY 402
           +FMED     P  VN+  ++ HA  V  S+LGE+NV+L P  MGAEDFGF +Q+I A  +
Sbjct: 308 DFMEDKLPPYPATVNDEEMYAHAKAVAESMLGEANVKLSPQGMGAEDFGFYAQRIPAAFF 367

Query: 403 GIGIRND-----TLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN 443
           GIG+ ND        +   LHSPHFV+DEEALP+GAA HAAVA+ YL  N
Sbjct: 368 GIGVGNDGGGMAETTTKNQLHSPHFVVDEEALPVGAAFHAAVAIEYLNKN 411

BLAST of CmoCh07G008650 vs. ExPASy Swiss-Prot
Match: Q851L6 (IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL4 PE=2 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 4.6e-127
Identity = 229/411 (55.72%), Postives = 295/411 (71.78%), Query Frame = 0

Query: 43  SLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAK 102
           +L R+LL  AR  EF  W++G+RRRIH++PE+ F+E+RTS LVR+ELD LG++Y WP+A+
Sbjct: 4   TLGRELLEAARAPEFAGWLRGLRRRIHQHPELAFQEHRTSALVRAELDALGVAYVWPIAQ 63

Query: 103 TGVVASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVA 162
           TGVVA++ GA+         PVFALRADMDALP+QE+VEWEFKS  +GKMHACGHD+HVA
Sbjct: 64  TGVVATVAGAAGPG------PVFALRADMDALPIQEMVEWEFKSLEDGKMHACGHDAHVA 123

Query: 163 MLLGAARLLQSKIEKLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTG 222
           MLL AA+LLQS+ +   G VKLVFQP E   G Y +L+E  LDD   IFA+HV   LP G
Sbjct: 124 MLLVAAKLLQSRRDHFNGKVKLVFQPAEGGAGGYHVLKEGVLDDTQTIFAVHVATDLPAG 183

Query: 223 VIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEA 282
           V+ SRPGP  AG+  F+A I GKGGHAA PH   DP++A +  + +LQQIV+RET+PL+ 
Sbjct: 184 VVGSRPGPFLAGSARFTATITGKGGHAAEPHLAVDPIVAASSAVLSLQQIVARETNPLQG 243

Query: 283 GVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVE 342
            VV+V  + GG+A NV+PE+V +GGT RS++  GLSYL  RIREVI  QA V+ C A+V+
Sbjct: 244 AVVSVTTIKGGEAFNVIPESVTLGGTLRSMTTDGLSYLMNRIREVIEGQAAVNRCTAAVD 303

Query: 343 FMEDT----PVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYG 402
           FMED     P  VN+  ++ HA  V  S+LGE+NV + P  MGAEDFGF +Q+I A  +G
Sbjct: 304 FMEDKLRPYPATVNDEGMYAHAKAVAESMLGEANVTVSPMCMGAEDFGFYAQRIPAAFFG 363

Query: 403 IGIRND-------TLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN 443
           IG+ ++          +   LHSPHFV+DEEALP+GAA HAAVA+ YL  N
Sbjct: 364 IGVGSNGNDGGGMAETTKNQLHSPHFVVDEEALPVGAAFHAAVAIEYLNKN 408

BLAST of CmoCh07G008650 vs. ExPASy Swiss-Prot
Match: Q8H3C9 (IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL7 PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 7.4e-125
Identity = 226/399 (56.64%), Postives = 287/399 (71.93%), Query Frame = 0

Query: 47  KLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVV 106
           +LL  AR   F  W++G+RR IH +PE+ FEE RTS+LVR+ELD +G+ Y+WPVA+TGVV
Sbjct: 52  ELLSAARAPGFAAWLRGLRRSIHRHPELAFEEVRTSELVRAELDAIGVPYEWPVARTGVV 111

Query: 107 ASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLG 166
           A+I G   + + +    VFALRADMDALPLQELV+WE KS+  GKMHACGHD+H  MLLG
Sbjct: 112 ATIAGGDGAGAGT----VFALRADMDALPLQELVDWEHKSEESGKMHACGHDAHTTMLLG 171

Query: 167 AARLLQSKIEKLKGTVKLVFQPGEE-CNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIA 226
           AA+LLQS+ + LKGTVKLVFQP EE   GA  +LQE  LDD+  IF LHV P +  G + 
Sbjct: 172 AAKLLQSQKDDLKGTVKLVFQPAEEGYAGARYVLQEGVLDDVSAIFGLHVDPRIQVGTVT 231

Query: 227 SRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVV 286
           SRPGP  A +G F A I GKGGHAA PH   DP+L  +  I +LQQIV+RETDPLEA V+
Sbjct: 232 SRPGPFLAASGRFLATITGKGGHAAGPHNAVDPILTASSAIVSLQQIVARETDPLEAAVI 291

Query: 287 TVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFME 346
           +V F+ GG A NV+PE+V  GGTFRSL+ +GLSYLK+RI+E++   A VH C A+V+FME
Sbjct: 292 SVTFMKGGDAYNVIPESVSFGGTFRSLTSEGLSYLKKRIKEIVEAHATVHRCTATVDFME 351

Query: 347 DT----PVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGI 406
           +     P  VN+  ++ HA  V   +LGE  V++    MG+EDF F +Q+  A  + IG+
Sbjct: 352 EERIPYPATVNDEGMYRHARAVAVDVLGEDGVKVGTPFMGSEDFAFYAQRFPAAFFMIGV 411

Query: 407 RND-TLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 440
            N+ T+R   PLHSPHFV+DE+ LP+GAALHAAVAM YL
Sbjct: 412 GNETTMRKVYPLHSPHFVVDEDVLPVGAALHAAVAMEYL 446

BLAST of CmoCh07G008650 vs. ExPASy Swiss-Prot
Match: Q8H3C8 (IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL8 PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 9.7e-125
Identity = 223/408 (54.66%), Postives = 295/408 (72.30%), Query Frame = 0

Query: 41  LHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPV 100
           L +L   LL  A    F  W+ G+RRRIH+ PE+ F+E RTS+LVR+ELD +G+ Y WPV
Sbjct: 37  LKALGEDLLAAAGAAGFAGWLSGLRRRIHQRPELAFQEVRTSELVRAELDAIGVPYAWPV 96

Query: 101 AKTGVVASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSH 160
           A+TGVVA+I G + +       PV ALRADMDALPLQELV+WEFKS+ +GKMHACGHD+H
Sbjct: 97  ARTGVVATIDGGAGAG------PVVALRADMDALPLQELVDWEFKSQEKGKMHACGHDAH 156

Query: 161 VAMLLGAARLLQSKIEKLKGTVKLVFQPGEECN-GAYQLLQEDALDDIDGIFALHVLPTL 220
           V MLLGAA+LLQS+ ++LKGT+KLVFQP EE + GAY +L+   LDD+  IF LHV+P L
Sbjct: 157 VTMLLGAAKLLQSRKDELKGTIKLVFQPAEEGHAGAYHVLESGLLDDVSVIFGLHVIPNL 216

Query: 221 PTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDP 280
           P GV+ASRPGP  + A  F+A   GKGGHA  PH   DPV+A +  + +LQQ+VSRETDP
Sbjct: 217 PVGVVASRPGPFMSAAARFAATFTGKGGHAGVPHDAVDPVVAVSSAVLSLQQLVSRETDP 276

Query: 281 LEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYA 340
           LEA VV++  + GG A NV+PE+  +GGTFRS++ +GL+YL +RIRE+I  QA V+ C A
Sbjct: 277 LEAAVVSITILKGGDAYNVIPESASLGGTFRSMTDEGLAYLMKRIREIIEAQAGVNRCAA 336

Query: 341 SVEFMEDT----PVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAAT 400
           +V+F+E+     P  VN+  ++ HA  V  ++LGE+NV++   +MG EDF F +++    
Sbjct: 337 AVDFLEEELRPYPATVNDDGMYGHAKAVAEAMLGEANVRVAARSMGGEDFAFYARRSPGA 396

Query: 401 IYGIGIRNDT----LRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 440
            + IG+ N+T      + RP+HSPHFVLDE ALP+GAALHAAVA+ YL
Sbjct: 397 FFFIGVGNETTMGPAAAVRPVHSPHFVLDERALPVGAALHAAVAIEYL 438

BLAST of CmoCh07G008650 vs. ExPASy TrEMBL
Match: A0A6J1HEG5 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111463223 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.3e-253
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW 60
           MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW
Sbjct: 21  MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW 80

Query: 61  IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSS 120
           IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSS
Sbjct: 81  IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSS 140

Query: 121 SRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKG 180
           SRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKG
Sbjct: 141 SRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKG 200

Query: 181 TVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSA 240
           TVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSA
Sbjct: 201 TVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSA 260

Query: 241 LIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVP 300
           LIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVP
Sbjct: 261 LIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVP 320

Query: 301 ETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHA 360
           ETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHA
Sbjct: 321 ETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHA 380

Query: 361 NRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLD 420
           NRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLD
Sbjct: 381 NRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLD 440

Query: 421 EEALPIGAALHAAVAMSYLEHN 443
           EEALPIGAALHAAVAMSYLEHN
Sbjct: 441 EEALPIGAALHAAVAMSYLEHN 462

BLAST of CmoCh07G008650 vs. ExPASy TrEMBL
Match: A0A6J1EDS0 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC111433244 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 6.7e-230
Identity = 402/437 (91.99%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 6   GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 65
           GGGM FKLFCWML+FGVALWRPSA+  EHGGDG ELHSLT+KL+GLAREG+F+EWIKGVR
Sbjct: 3   GGGMVFKLFCWMLLFGVALWRPSAA--EHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 62

Query: 66  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 125
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASI GA    S SSS PVF
Sbjct: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGA----SLSSSMPVF 122

Query: 126 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 185
           ALRADMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLV
Sbjct: 123 ALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLV 182

Query: 186 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 245
           FQPGEECNGAYQ+LQEDALD+IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 183 FQPGEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGK 242

Query: 246 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 305
           GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQA+NVVPETVKV
Sbjct: 243 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKV 302

Query: 306 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 365
           GGTFRSLSP+GLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGN
Sbjct: 303 GGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGN 362

Query: 366 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 425
           SLLGESNVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TL S+RPLHSPHFVLDEEALP
Sbjct: 363 SLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALP 422

Query: 426 IGAALHAAVAMSYLEHN 443
           IGAALHAAVAMSYLEHN
Sbjct: 423 IGAALHAAVAMSYLEHN 433

BLAST of CmoCh07G008650 vs. ExPASy TrEMBL
Match: A0A6J1IVY2 (IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.4e-227
Identity = 400/437 (91.53%), Postives = 419/437 (95.88%), Query Frame = 0

Query: 6   GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 65
           GGGM+FKLFC ML FGVALWRPSA+  EHGGDG +LHSLT+KL+GLAREGEF+EWIKGVR
Sbjct: 3   GGGMDFKLFCSMLFFGVALWRPSAA--EHGGDGSDLHSLTQKLMGLAREGEFMEWIKGVR 62

Query: 66  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 125
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASI GAS SSS+    PVF
Sbjct: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGASLSSST----PVF 122

Query: 126 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 185
           ALRADMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLL+SK  KLKGTVKLV
Sbjct: 123 ALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLRSKRLKLKGTVKLV 182

Query: 186 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 245
           FQPGEECNGAYQ+LQEDALD+IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 183 FQPGEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGK 242

Query: 246 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 305
           GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQA+NVVPETVKV
Sbjct: 243 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKV 302

Query: 306 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 365
           GGTFRSLSP+GLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGN
Sbjct: 303 GGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNEALFEHANRVGN 362

Query: 366 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 425
           SLLGESNVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TL S+RPLHSPHFVLDEEALP
Sbjct: 363 SLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALP 422

Query: 426 IGAALHAAVAMSYLEHN 443
           IGAALHAAVAMSYLEHN
Sbjct: 423 IGAALHAAVAMSYLEHN 433

BLAST of CmoCh07G008650 vs. ExPASy TrEMBL
Match: A0A6J1BV66 (IAA-amino acid hydrolase ILR1-like 3 OS=Momordica charantia OX=3673 GN=LOC111005645 PE=3 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.0e-217
Identity = 381/430 (88.60%), Postives = 406/430 (94.42%), Query Frame = 0

Query: 13  LFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYP 72
           LFCWM+  G+AL R SA+    GGDG ELHSLTR LLG AREGEF+EWIKGVRRRIHEYP
Sbjct: 11  LFCWMVFLGMALSR-SAAAAARGGDGSELHSLTRNLLGSAREGEFMEWIKGVRRRIHEYP 70

Query: 73  EVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVFALRADMD 132
           E+GFEEYRTSQLVRSELD LGISYQWPVA+TGVVASIKGA   +S SSSRPVFALRADMD
Sbjct: 71  ELGFEEYRTSQLVRSELDSLGISYQWPVAETGVVASIKGA---ASLSSSRPVFALRADMD 130

Query: 133 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEEC 192
           ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLVFQPGEEC
Sbjct: 131 ALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKRDKLKGTVKLVFQPGEEC 190

Query: 193 NGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAP 252
           NGAY++L+++ALD IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGKGGHAA P
Sbjct: 191 NGAYKMLKDEALDGIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAIP 250

Query: 253 HKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSL 312
           H TKDPVLATAFIIQALQQIVSRETDPLEAGVVT+AFV+GGQA+N+VPETVKVGGTFRSL
Sbjct: 251 HMTKDPVLATAFIIQALQQIVSRETDPLEAGVVTIAFVEGGQAENIVPETVKVGGTFRSL 310

Query: 313 SPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGNSLLGESN 372
           SP+G SYLKERIREVINTQA VHHC+A+V+FMEDTPVMVNNVALFEHAN VGNSLLGESN
Sbjct: 311 SPEGFSYLKERIREVINTQAAVHHCHAAVKFMEDTPVMVNNVALFEHANMVGNSLLGESN 370

Query: 373 VQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHA 432
           VQLLPWTMGAEDFGF SQ+IAATI+GIG+RNDTLRSNRPLHSPHFVLDEEALPIGAALHA
Sbjct: 371 VQLLPWTMGAEDFGFFSQRIAATIFGIGVRNDTLRSNRPLHSPHFVLDEEALPIGAALHA 430

Query: 433 AVAMSYLEHN 443
           AVAMSYLEHN
Sbjct: 431 AVAMSYLEHN 436

BLAST of CmoCh07G008650 vs. ExPASy TrEMBL
Match: A0A0A0KIR8 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.1e-216
Identity = 380/437 (86.96%), Postives = 404/437 (92.45%), Query Frame = 0

Query: 6   GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 65
           GGG  FK  CWM+VFG+ALWRP+A   E+GGDG ELHSLT+ LLGLARE EF+EWIKGVR
Sbjct: 4   GGGTSFKSLCWMIVFGIALWRPAAG--ENGGDGSELHSLTQSLLGLAREREFMEWIKGVR 63

Query: 66  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 125
           RRIHEYPE+GFEEY+TSQLVRSELD LGISY+WPVAKTGVVASI+G   S SSSSS PVF
Sbjct: 64  RRIHEYPELGFEEYKTSQLVRSELDSLGISYRWPVAKTGVVASIRG--DSVSSSSSTPVF 123

Query: 126 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 185
            LRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAM+LGAARLLQS  EKLKGTVKLV
Sbjct: 124 GLRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMVLGAARLLQSIREKLKGTVKLV 183

Query: 186 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 245
           FQP EECNGAYQ+L++DALD IDGIFALHV P+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 184 FQPAEECNGAYQMLKDDALDGIDGIFALHVQPSLPTGVIASRPGPVCAGAGHFSALIRGK 243

Query: 246 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 305
           GGHAA PHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQA+NVVPETVKV
Sbjct: 244 GGHAATPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKV 303

Query: 306 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 365
           GGTFRSLSP+G SYLKERIREVI+TQA+VHHCYASV+FMEDTPVMVNN ALFEH NRVGN
Sbjct: 304 GGTFRSLSPEGFSYLKERIREVISTQAMVHHCYASVKFMEDTPVMVNNEALFEHVNRVGN 363

Query: 366 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 425
           SLLGESNVQLLPWTMGAEDFGF SQ+I ATIY IG  N+TL SNRP+HSPHFVLDEEALP
Sbjct: 364 SLLGESNVQLLPWTMGAEDFGFFSQRIPATIYVIGTGNETLGSNRPVHSPHFVLDEEALP 423

Query: 426 IGAALHAAVAMSYLEHN 443
           IGAALHAAVA +YLEHN
Sbjct: 424 IGAALHAAVATTYLEHN 436

BLAST of CmoCh07G008650 vs. NCBI nr
Match: XP_022962856.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata])

HSP 1 Score: 883.6 bits (2282), Expect = 6.9e-253
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW 60
           MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW
Sbjct: 21  MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW 80

Query: 61  IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSS 120
           IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSS
Sbjct: 81  IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSS 140

Query: 121 SRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKG 180
           SRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKG
Sbjct: 141 SRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKG 200

Query: 181 TVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSA 240
           TVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSA
Sbjct: 201 TVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSA 260

Query: 241 LIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVP 300
           LIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVP
Sbjct: 261 LIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVP 320

Query: 301 ETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHA 360
           ETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHA
Sbjct: 321 ETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHA 380

Query: 361 NRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLD 420
           NRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLD
Sbjct: 381 NRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLD 440

Query: 421 EEALPIGAALHAAVAMSYLEHN 443
           EEALPIGAALHAAVAMSYLEHN
Sbjct: 441 EEALPIGAALHAAVAMSYLEHN 462

BLAST of CmoCh07G008650 vs. NCBI nr
Match: KAG6595165.1 (IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 874.4 bits (2258), Expect = 4.2e-250
Identity = 441/448 (98.44%), Postives = 441/448 (98.44%), Query Frame = 0

Query: 1   MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW 60
           MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW
Sbjct: 1   MAGGGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEW 60

Query: 61  IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGA------SS 120
           IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGA      SS
Sbjct: 61  IKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGAAASSSSSS 120

Query: 121 SSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK 180
           SSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK
Sbjct: 121 SSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK 180

Query: 181 IEKLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAG 240
           IEKLKGTVKLVFQPGEECNGAYQLLQE ALDDIDGIFALHVLPTLPTGVIASRPGPVCAG
Sbjct: 181 IEKLKGTVKLVFQPGEECNGAYQLLQEHALDDIDGIFALHVLPTLPTGVIASRPGPVCAG 240

Query: 241 AGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQ 300
           AGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQ
Sbjct: 241 AGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQ 300

Query: 301 ADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNV 360
           ADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNV
Sbjct: 301 ADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNV 360

Query: 361 ALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHS 420
           ALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHS
Sbjct: 361 ALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHS 420

Query: 421 PHFVLDEEALPIGAALHAAVAMSYLEHN 443
           PHFVLDEEALPIGAALHAAVAMSYLEHN
Sbjct: 421 PHFVLDEEALPIGAALHAAVAMSYLEHN 448

BLAST of CmoCh07G008650 vs. NCBI nr
Match: XP_023517717.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 867.8 bits (2241), Expect = 3.9e-248
Identity = 437/448 (97.54%), Postives = 438/448 (97.77%), Query Frame = 0

Query: 4   GGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKG 63
           GGGGGMEFKLFCWMLVFGV LWRPSASEVEHGGDGLELHSLTR+LLGLAREGEFLEWIKG
Sbjct: 3   GGGGGMEFKLFCWMLVFGVVLWRPSASEVEHGGDGLELHSLTRRLLGLAREGEFLEWIKG 62

Query: 64  VRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGA---------SS 123
           VRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGA         SS
Sbjct: 63  VRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSSS 122

Query: 124 SSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK 183
           SSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK
Sbjct: 123 SSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSK 182

Query: 184 IEKLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAG 243
           IEKLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAG
Sbjct: 183 IEKLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAG 242

Query: 244 AGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQ 303
           AGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQ
Sbjct: 243 AGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQ 302

Query: 304 ADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNV 363
           ADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNV
Sbjct: 303 ADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNV 362

Query: 364 ALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHS 423
           ALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHS
Sbjct: 363 ALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHS 422

Query: 424 PHFVLDEEALPIGAALHAAVAMSYLEHN 443
           PHFVLDEEALPIGAALHAAVAMSYLEHN
Sbjct: 423 PHFVLDEEALPIGAALHAAVAMSYLEHN 450

BLAST of CmoCh07G008650 vs. NCBI nr
Match: KAG7027180.1 (IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 856.3 bits (2211), Expect = 1.2e-244
Identity = 438/467 (93.79%), Postives = 438/467 (93.79%), Query Frame = 0

Query: 4   GGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKG 63
           GGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKG
Sbjct: 3   GGGGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKG 62

Query: 64  VRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGA-------SSSS 123
           VRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGA       SSSS
Sbjct: 63  VRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGAAASSSSSSSSS 122

Query: 124 SSSSSRPVFALRADMDALPLQ---------------------ELVEWEFKSKVEGKMHAC 183
           SSSSSRPVFALRADMDALPLQ                     ELVEWEFKSKVEGKMHAC
Sbjct: 123 SSSSSRPVFALRADMDALPLQVLSINKDETGKWHTDQIQWKKELVEWEFKSKVEGKMHAC 182

Query: 184 GHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEECNGAYQLLQEDALDDIDGIFALHV 243
           GHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEECNGAYQLLQE ALDDIDGIFALHV
Sbjct: 183 GHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEECNGAYQLLQEHALDDIDGIFALHV 242

Query: 244 LPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSR 303
           LPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSR
Sbjct: 243 LPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSR 302

Query: 304 ETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVH 363
           ETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVH
Sbjct: 303 ETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVH 362

Query: 364 HCYASVEFMEDTPVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAAT 423
           HCYASVEFMEDTPVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAAT
Sbjct: 363 HCYASVEFMEDTPVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAAT 422

Query: 424 IYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN 443
           IYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN
Sbjct: 423 IYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLEHN 469

BLAST of CmoCh07G008650 vs. NCBI nr
Match: XP_022925994.1 (IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034481.1 IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 806.2 bits (2081), Expect = 1.4e-229
Identity = 402/437 (91.99%), Postives = 420/437 (96.11%), Query Frame = 0

Query: 6   GGGMEFKLFCWMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVR 65
           GGGM FKLFCWML+FGVALWRPSA+  EHGGDG ELHSLT+KL+GLAREG+F+EWIKGVR
Sbjct: 3   GGGMVFKLFCWMLLFGVALWRPSAA--EHGGDGSELHSLTQKLMGLAREGDFMEWIKGVR 62

Query: 66  RRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVF 125
           RRIHEYPE+GFEEYRTSQLVRSELD LGISYQWPVAKTGVVASI GA    S SSS PVF
Sbjct: 63  RRIHEYPELGFEEYRTSQLVRSELDSLGISYQWPVAKTGVVASITGA----SLSSSMPVF 122

Query: 126 ALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLV 185
           ALRADMDALPLQELVEWEFKSKV+GKMHACGHDSHVAMLLGAARLLQSK +KLKGTVKLV
Sbjct: 123 ALRADMDALPLQELVEWEFKSKVKGKMHACGHDSHVAMLLGAARLLQSKRQKLKGTVKLV 182

Query: 186 FQPGEECNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGK 245
           FQPGEECNGAYQ+LQEDALD+IDGIFALHVLP+LPTGVIASRPGPVCAGAGHFSALIRGK
Sbjct: 183 FQPGEECNGAYQMLQEDALDNIDGIFALHVLPSLPTGVIASRPGPVCAGAGHFSALIRGK 242

Query: 246 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKV 305
           GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQA+NVVPETVKV
Sbjct: 243 GGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQAENVVPETVKV 302

Query: 306 GGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFMEDTPVMVNNVALFEHANRVGN 365
           GGTFRSLSP+GLSYLKERIREVIN QA+VHHCYASVEFMEDTPVMVNN ALFEHANRVGN
Sbjct: 303 GGTFRSLSPEGLSYLKERIREVINKQAIVHHCYASVEFMEDTPVMVNNKALFEHANRVGN 362

Query: 366 SLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALP 425
           SLLGESNVQLLPWTMGAEDFGF SQ+IAATIYGIGIRN+TL S+RPLHSPHFVLDEEALP
Sbjct: 363 SLLGESNVQLLPWTMGAEDFGFFSQRIAATIYGIGIRNETLGSSRPLHSPHFVLDEEALP 422

Query: 426 IGAALHAAVAMSYLEHN 443
           IGAALHAAVAMSYLEHN
Sbjct: 423 IGAALHAAVAMSYLEHN 433

BLAST of CmoCh07G008650 vs. TAIR 10
Match: AT3G02875.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 488.4 bits (1256), Expect = 6.0e-138
Identity = 242/432 (56.02%), Postives = 317/432 (73.38%), Query Frame = 0

Query: 16  WMLVFGVALWRPSASEVEHGGDGLELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVG 75
           + ++F    + P  S       G  L SL R +L  A++ EF EW++G+RR+IHE PE G
Sbjct: 7   FFVIFVTFFFLPPLSSAGSYDSGSGLESLARGMLHSAKDPEFFEWMRGIRRKIHENPETG 66

Query: 76  FEEYRTSQLVRSELDLLGISYQWPVAKTGVVASIKGASSSSSSSSSRPVFALRADMDALP 135
           F+E++TSQLVR ELD LG+ Y++PVAKTGVVA I         S S+PVF LRADMDALP
Sbjct: 67  FQEFKTSQLVRDELDSLGVKYKYPVAKTGVVAWI--------GSCSKPVFGLRADMDALP 126

Query: 136 LQELVEWEFKSKVEGKMHACGHDSHVAMLLGAARLLQSKIEKLKGTVKLVFQPGEE-CNG 195
           LQELVEWE KSKV+GKMHACGHD+HVAMLLGAA+LLQ+    +KGTVKLVFQPGEE   G
Sbjct: 127 LQELVEWESKSKVDGKMHACGHDTHVAMLLGAAKLLQTTKHLIKGTVKLVFQPGEEGYAG 186

Query: 196 AYQLLQEDALDDIDGIFALHVLPTLPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHK 255
           AY++L+++ LDD+DGI ++HV P++P+G I SRPG V AGAG F+  + G+G HAA PH 
Sbjct: 187 AYEMLKDEILDDLDGILSVHVFPSIPSGGIGSRPGTVLAGAGLFTVTVHGQGSHAATPHF 246

Query: 256 TKDPVLATAFIIQALQQIVSRETDPLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSP 315
           +KDPVLA +  + ALQQIVSRE DPLEAGVVTV +++GG A NV+P++ K GGTFRSLS 
Sbjct: 247 SKDPVLAASSAVVALQQIVSRELDPLEAGVVTVGYIEGGHAQNVIPQSAKFGGTFRSLSN 306

Query: 316 KGLSYLKERIREVINTQAVVHHCYASVEFMEDT----PVMVNNVALFEHANRVGNSLLGE 375
            GL +++ RI+E+   QA V+ C A V F E      PVM N+  L+EH  +V  +++G+
Sbjct: 307 DGLLFIQRRIKEISEAQASVYRCKAEVNFEEKKPSLHPVMNNDEGLYEHGKKVAEAMIGK 366

Query: 376 SNVQLLPWTMGAEDFGFLSQKIAATIYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAAL 435
           +N    P TMG EDF F +QK  A I+ +G++N+TL + +PLHSP+F +DEEALP+GAAL
Sbjct: 367 NNFHDFPVTMGGEDFSFFTQKTKAAIFVLGVKNETLGAGKPLHSPYFFVDEEALPVGAAL 426

Query: 436 HAAVAMSYLEHN 443
           HAA+A+SYL+ +
Sbjct: 427 HAAMAVSYLDEH 430

BLAST of CmoCh07G008650 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 373.6 bits (958), Expect = 2.2e-103
Identity = 197/404 (48.76%), Postives = 266/404 (65.84%), Query Frame = 0

Query: 41  LHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPV 100
           L  +  K L LA+  +F +W+ G+RRRIHE PE+G+EE  TS+LVR+EL+ +G+SY++PV
Sbjct: 28  LSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPELGYEEVETSKLVRAELEKMGVSYKYPV 87

Query: 101 AKTGVVASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSH 160
           A TGVV  +         +   P  ALRADMDAL +QE+VEWE KSKV GKMHACGHD+H
Sbjct: 88  AVTGVVGYV--------GTGHAPFVALRADMDALAMQEMVEWEHKSKVPGKMHACGHDAH 147

Query: 161 VAMLLGAARLLQSKIEKLKGTVKLVFQPGEE-CNGAYQLLQEDALDDIDGIFALHVLPTL 220
             MLLGAA+LL+   E+L+GTV LVFQP EE   GA ++++   L+++  IF LHV   L
Sbjct: 148 TTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEAGVLENVSAIFGLHVTNQL 207

Query: 221 PTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDP 280
             G ++SR GP+ AG+G F A I GKGGHAA P  T DP+LA + +I +LQ +VSRE DP
Sbjct: 208 ALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILAASNVIVSLQHLVSREADP 267

Query: 281 LEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYA 340
           L++ VVTVA  +GG A NV+P++V +GGTFR+ S K    LK+RI +VI  QA V+ C A
Sbjct: 268 LDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLKKRIEQVITRQASVNMCNA 327

Query: 341 SVEFMEDT----PVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAAT 400
           +V+F+E+     P  VN+ AL +    V   +LG  N   +   MG+EDF F  Q I   
Sbjct: 328 TVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQPLMGSEDFSFYQQAIPGH 387

Query: 401 IYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 440
              +G++N         HSP+F ++EE LP GA+LHA++A  YL
Sbjct: 388 FSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATRYL 423

BLAST of CmoCh07G008650 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 369.4 bits (947), Expect = 4.1e-102
Identity = 202/398 (50.75%), Postives = 263/398 (66.08%), Query Frame = 0

Query: 47  KLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVV 106
           ++L L  + + + W+K VRR IHE PE+ FEEY TS+L+RSELD +GI Y++P+AKTG+ 
Sbjct: 75  EILRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIR 134

Query: 107 ASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLG 166
           A I         S   P  A+RADMDALP+QE VEWE  SKV GKMHACGHD+HV MLLG
Sbjct: 135 AWI--------GSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLG 194

Query: 167 AARLLQSKIEKLKGTVKLVFQPGEEC-NGAYQLLQEDALDDIDGIFALHVLPTLPTGVIA 226
           AA +L+++   LKGTV L+FQP EE  NGA  ++++ ALDD++ IFA+HV    PTGVI 
Sbjct: 195 AAHILKAREHLLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIG 254

Query: 227 SRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVV 286
           SR GP+ AG G F A+I  +    AA     + +LA +  + +LQ IVSRE  PL++ VV
Sbjct: 255 SRSGPLLAGCGIFRAVITSEDSRGAA-----NLLLAASSAVISLQGIVSREASPLDSQVV 314

Query: 287 TVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEFME 346
           +V   DGG + +V P+TV +GGTFR+ S     YLK+RI+EV+  Q  V  C A+V F E
Sbjct: 315 SVTSFDGGHSLDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFE 374

Query: 347 DT----PVMVNNVALFEHANRVGNSLLGESNVQLLPWTMGAEDFGFLSQKIAATIYGIGI 406
                 P   NN A + H  +V   LLG+S+  L P  MGAEDF F S+ I A  Y IGI
Sbjct: 375 KQNAIYPPTTNNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGI 434

Query: 407 RNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 440
           RN+ L S    HSPHF++DE++LP+GAA+HAAVA  YL
Sbjct: 435 RNEELGSVHIAHSPHFMIDEDSLPVGAAVHAAVAERYL 459

BLAST of CmoCh07G008650 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 360.9 bits (925), Expect = 1.4e-99
Identity = 197/406 (48.52%), Postives = 266/406 (65.52%), Query Frame = 0

Query: 40  ELHSLTRKLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWP 99
           +L  + +  L LA+  +F +W+ G+RRRIHE PE+G+EE  TS+LV++ELD +G+SY+ P
Sbjct: 27  DLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGVSYKNP 86

Query: 100 VAKTGVVASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDS 159
           VA TGV+  +         +   P  ALRADMDALP+QE+VEWE KSK+ GKMHACGHD+
Sbjct: 87  VAVTGVIGYV--------GTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDA 146

Query: 160 HVAMLLGAARLLQSKIEKLKGTVKLVFQPGEECN-GAYQLLQEDALDDIDGIFALHVLPT 219
           H  MLLGAA+LL+   E+L+GTV LVFQP EE   GA ++++   L+++  IF LHV   
Sbjct: 147 HTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNL 206

Query: 220 LPTGVIASRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETD 279
           L  G ++SR G + AG+G F A I GKGGHAA P    DPVLA + +I +LQ +VSRE D
Sbjct: 207 LGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREAD 266

Query: 280 PLEAGVVTVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCY 339
           PL++ VVTVA  +G  A NV+P++V +GGTFR+L PK    LK+RI +VI TQA V+ C 
Sbjct: 267 PLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCN 326

Query: 340 ASVEFMEDT----PVMVNNVALFEHANRVGNSLLG-ESNVQLLPWTMGAEDFGFLSQKIA 399
           A+V+F+ED     P  VNN  L      V   +LG E+ V+ LP  M +EDF F  Q I 
Sbjct: 327 ATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLP-VMVSEDFAFYQQAIP 386

Query: 400 ATIYGIGIRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYL 440
                +G++N +       HSP F ++EE LP GA+L A++A  YL
Sbjct: 387 GHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYL 423

BLAST of CmoCh07G008650 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 357.8 bits (917), Expect = 1.2e-98
Identity = 192/400 (48.00%), Postives = 268/400 (67.00%), Query Frame = 0

Query: 47  KLLGLAREGEFLEWIKGVRRRIHEYPEVGFEEYRTSQLVRSELDLLGISYQWPVAKTGVV 106
           KLL  A+  E  +W+  +RR+IHE PE+G+EE  TS+L+RSEL+L+GI Y++PVA TGV+
Sbjct: 37  KLLEFAKSPEVFDWMVKIRRKIHENPELGYEELETSKLIRSELELIGIKYRYPVAITGVI 96

Query: 107 ASIKGASSSSSSSSSRPVFALRADMDALPLQELVEWEFKSKVEGKMHACGHDSHVAMLLG 166
             I         +   P  ALRADMDALP+QE VEWE KSK+ GKMHACGHD HV MLLG
Sbjct: 97  GYI--------GTGEPPFVALRADMDALPIQEGVEWEHKSKIAGKMHACGHDGHVTMLLG 156

Query: 167 AARLLQSKIEKLKGTVKLVFQPGEE-CNGAYQLLQEDALDDIDGIFALHVLPTLPTGVIA 226
           AA++L      L+GTV L+FQP EE  +GA ++ +E AL +++ IF +H+   +P G  A
Sbjct: 157 AAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGALKNVEAIFGIHLSARIPFGKAA 216

Query: 227 SRPGPVCAGAGHFSALIRGKGGHAAAPHKTKDPVLATAFIIQALQQIVSRETDPLEAGVV 286
           SR G   AGAG F A+I GKGGHAA P  T DPV+A + I+ +LQQ+VSRETDPL++ VV
Sbjct: 217 SRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAASSIVLSLQQLVSRETDPLDSKVV 276

Query: 287 TVAFVDGGQADNVVPETVKVGGTFRSLSPKGLSYLKERIREVINTQAVVHHCYASVEF-- 346
           TV+ V+GG A NV+P+++ +GGT R+ +  G + L++R++EVI  QA VH C ASV    
Sbjct: 277 TVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQRVKEVITKQAAVHRCNASVNLTP 336

Query: 347 --MEDTPVMVNNVALFEHANRVGNSLLG-ESNVQLLPWTMGAEDFGFLSQKIAATIYGIG 406
              E  P  VNN  L++   +V   LLG E+ V+  P  MG+EDF + ++ I      +G
Sbjct: 337 NGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAP-VMGSEDFSYFAETIPGHFSLLG 396

Query: 407 IRNDTLRSNRPLHSPHFVLDEEALPIGAALHAAVAMSYLE 441
           ++++T       HSP + ++E+ LP GAA+HA++A+ YL+
Sbjct: 397 MQDET-NGYASSHSPLYRINEDVLPYGAAIHASMAVQYLK 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P549688.4e-13756.02IAA-amino acid hydrolase ILR1 OS=Arabidopsis thaliana OX=3702 GN=ILR1 PE=1 SV=2[more]
Q851L55.3e-13157.56IAA-amino acid hydrolase ILR1-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q851L64.6e-12755.72IAA-amino acid hydrolase ILR1-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C97.4e-12556.64IAA-amino acid hydrolase ILR1-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q8H3C89.7e-12554.66IAA-amino acid hydrolase ILR1-like 8 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1HEG53.3e-253100.00IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114632... [more]
A0A6J1EDS06.7e-23091.99IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita moschata OX=3662 GN=LOC1114332... [more]
A0A6J1IVY21.4e-22791.53IAA-amino acid hydrolase ILR1-like 3 OS=Cucurbita maxima OX=3661 GN=LOC111478889... [more]
A0A6J1BV661.0e-21788.60IAA-amino acid hydrolase ILR1-like 3 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A0A0KIR81.1e-21686.96M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G454350 P... [more]
Match NameE-valueIdentityDescription
XP_022962856.16.9e-253100.00IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata][more]
KAG6595165.14.2e-25098.44IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023517717.13.9e-24897.54IAA-amino acid hydrolase ILR1-like 3 [Cucurbita pepo subsp. pepo][more]
KAG7027180.11.2e-24493.79IAA-amino acid hydrolase ILR1, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022925994.11.4e-22991.99IAA-amino acid hydrolase ILR1-like 3 [Cucurbita moschata] >KAG6604330.1 IAA-amin... [more]
Match NameE-valueIdentityDescription
AT3G02875.16.0e-13856.02Peptidase M20/M25/M40 family protein [more]
AT1G51760.12.2e-10348.76peptidase M20/M25/M40 family protein [more]
AT1G44350.14.1e-10250.75IAA-leucine resistant (ILR)-like gene 6 [more]
AT1G51780.11.4e-9948.52IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56660.11.2e-9848.00IAA-leucine resistant (ILR)-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 50..442
e-value: 1.2E-113
score: 377.9
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 63..427
e-value: 1.6E-110
score: 367.6
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 12..441
NoneNo IPR availableGENE3D3.30.70.360coord: 233..347
e-value: 7.0E-127
score: 425.1
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 64..424
e-value: 7.0E-127
score: 425.1
NoneNo IPR availablePANTHERPTHR11014:SF108IAA-AMINO ACID HYDROLASE ILR1coord: 12..441
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 56..440
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 242..333
e-value: 6.4E-10
score: 38.9
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 126..435
e-value: 1.0E-30
score: 107.5
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 61..439
e-value: 0.0
score: 577.348
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 236..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh07G008650.1CmoCh07G008650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
molecular_function GO:0010179 IAA-Ala conjugate hydrolase activity
molecular_function GO:0016787 hydrolase activity