CmoCh06G014440 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G014440
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Guanylate kinase
Location	Cmo_Chr06: 10455265 .. 10456152 (+)
RNA-Seq Expression	CmoCh06G014440
Synteny	CmoCh06G014440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTGCAGATTCTTCGGCGCCTATCTTTCCCGCTCTGGAAATCTCCGCATAAAATTCCACAACCCCATTTCCCTTACCAATTTCCTCAACCCCAAATCAATCAAATCCAAACCCATTCAACCATTCCCTCGAACCCCAACATTTTTCTCATCCAGTTCTAAAATGGGTGATGCTCGAAGACCGCTCTCCGCCAACATTCCGCCACCAGACAAAGCCGAACGATCGGAATTGCTGCGATCACTCGAGTTTTCGCTCAATTCTGGGTTTAGCTCCGAGCCATTGTTACCGAAACCGAGCCCTTTGATTATTGTTATCAGCGGTCCAAGTGGGGTGGGGAAGGACGCTGTGATTAAACGATTGAGAGAGGTTCGAGAAGGGCTACGTTTTGTCGTGACAGCGACGAGTCGGTCAATGCGACCTGGTGAAGTCGATGGAGAAGACTATTATTTCGTCTCTAAGGAAGAATTTCTGGCCATGATTGAGAGGAACGAGCTTCTGGAATATGCGTTAGTGTATGGGGATTACAAGGGCATTCCCAAAAGGCAAATTAGGGAATTCATGGCACAAGGGTTTGATATTGTGTTGAGAGTAGACATTCAAGGGGCCGAGACATTAAGGAAAATCCTTGGAAAATCTGCTGTGTTTGTGTTCTTGATGGCGGAGAGTGAGCTTAAACTGGTGGAGAGGTTGATTGATAGGAAGACGGAGACGAAGGAGTCATTGTTGGTGAGAGTTGCAACGGCTAGAGAGGAAGTAAGACATGTTAAGAATTTTGATTATGTGGTTGTGAATGCAGAAGGGAAGCTGGAGAATGCGGCTAAGCTGGTGGGATCCATTATTGATGCTGAGAAAGCAAAGGTCTGGCAGAGAAATGCTTCCGTTTAG

mRNA sequence

ATGTCTTGCAGATTCTTCGGCGCCTATCTTTCCCGCTCTGGAAATCTCCGCATAAAATTCCACAACCCCATTTCCCTTACCAATTTCCTCAACCCCAAATCAATCAAATCCAAACCCATTCAACCATTCCCTCGAACCCCAACATTTTTCTCATCCAGTTCTAAAATGGGTGATGCTCGAAGACCGCTCTCCGCCAACATTCCGCCACCAGACAAAGCCGAACGATCGGAATTGCTGCGATCACTCGAGTTTTCGCTCAATTCTGGGTTTAGCTCCGAGCCATTGTTACCGAAACCGAGCCCTTTGATTATTGTTATCAGCGGTCCAAGTGGGGTGGGGAAGGACGCTGTGATTAAACGATTGAGAGAGGTTCGAGAAGGGCTACGTTTTGTCGTGACAGCGACGAGTCGGTCAATGCGACCTGGTGAAGTCGATGGAGAAGACTATTATTTCGTCTCTAAGGAAGAATTTCTGGCCATGATTGAGAGGAACGAGCTTCTGGAATATGCGTTAGTGTATGGGGATTACAAGGGCATTCCCAAAAGGCAAATTAGGGAATTCATGGCACAAGGGTTTGATATTGTGTTGAGAGTAGACATTCAAGGGGCCGAGACATTAAGGAAAATCCTTGGAAAATCTGCTGTGTTTGTGTTCTTGATGGCGGAGAGTGAGCTTAAACTGGTGGAGAGGTTGATTGATAGGAAGACGGAGACGAAGGAGTCATTGTTGGTGAGAGTTGCAACGGCTAGAGAGGAAGTAAGACATGTTAAGAATTTTGATTATGTGGTTGTGAATGCAGAAGGGAAGCTGGAGAATGCGGCTAAGCTGGTGGGATCCATTATTGATGCTGAGAAAGCAAAGGTCTGGCAGAGAAATGCTTCCGTTTAG

Coding sequence (CDS)

ATGTCTTGCAGATTCTTCGGCGCCTATCTTTCCCGCTCTGGAAATCTCCGCATAAAATTCCACAACCCCATTTCCCTTACCAATTTCCTCAACCCCAAATCAATCAAATCCAAACCCATTCAACCATTCCCTCGAACCCCAACATTTTTCTCATCCAGTTCTAAAATGGGTGATGCTCGAAGACCGCTCTCCGCCAACATTCCGCCACCAGACAAAGCCGAACGATCGGAATTGCTGCGATCACTCGAGTTTTCGCTCAATTCTGGGTTTAGCTCCGAGCCATTGTTACCGAAACCGAGCCCTTTGATTATTGTTATCAGCGGTCCAAGTGGGGTGGGGAAGGACGCTGTGATTAAACGATTGAGAGAGGTTCGAGAAGGGCTACGTTTTGTCGTGACAGCGACGAGTCGGTCAATGCGACCTGGTGAAGTCGATGGAGAAGACTATTATTTCGTCTCTAAGGAAGAATTTCTGGCCATGATTGAGAGGAACGAGCTTCTGGAATATGCGTTAGTGTATGGGGATTACAAGGGCATTCCCAAAAGGCAAATTAGGGAATTCATGGCACAAGGGTTTGATATTGTGTTGAGAGTAGACATTCAAGGGGCCGAGACATTAAGGAAAATCCTTGGAAAATCTGCTGTGTTTGTGTTCTTGATGGCGGAGAGTGAGCTTAAACTGGTGGAGAGGTTGATTGATAGGAAGACGGAGACGAAGGAGTCATTGTTGGTGAGAGTTGCAACGGCTAGAGAGGAAGTAAGACATGTTAAGAATTTTGATTATGTGGTTGTGAATGCAGAAGGGAAGCTGGAGAATGCGGCTAAGCTGGTGGGATCCATTATTGATGCTGAGAAAGCAAAGGTCTGGCAGAGAAATGCTTCCGTTTAG

Protein sequence

MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDARRPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKRLREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKESLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV

Homology

[Close]

BLAST of CmoCh06G014440 vs. ExPASy Swiss-Prot
Match: Q94JM2 (Guanylate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GK3 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.3e-90
Identity = 172/251 (68.53%), Postives = 208/251 (82.87%), Query Frame = 0


Query: 41  QPFPRTPTFFSSSSKMGDARRPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPS 100
           QP   +     SS KM D   P + N  P     + E LRSLEF L S F+++P++P P+
Sbjct: 28  QPLRHSSALSYSSLKMTDTHIPGNPNSSPIQSPTKPESLRSLEFQLGSSFTADPIIPPPN 87

Query: 101 PLIIVISGPSGVGKDAVIKRLREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAM 160
            ++IVISGPSGVGKDAVI +LREVREGL FVVTATSR MRPGEVDG+DY+FVS+++FL+M
Sbjct: 88  QIVIVISGPSGVGKDAVINKLREVREGLHFVVTATSRPMRPGEVDGKDYFFVSRDQFLSM 147

Query: 161 IERNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLM 220
           +E  ELLEYALVYG+YKGIPK+QI+EFMA+G DIVLRVDIQGA+TLR+ILG SAVF+FL+
Sbjct: 148 VENEELLEYALVYGEYKGIPKKQIQEFMAKGEDIVLRVDIQGAQTLRRILGNSAVFIFLV 207

Query: 221 AESELKLVERLIDRKTETKESLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSI 280
           AESEL +VERLIDRKTE++E LLVRVATAREEVRH+KNFDYVVVNA+G+L++A   V SI
Sbjct: 208 AESELAMVERLIDRKTESQEELLVRVATAREEVRHLKNFDYVVVNAKGRLDDAVNRVESI 267

Query: 281 IDAEKAKVWQR 292
           IDAEK+KV QR
Sbjct: 268 IDAEKSKVHQR 278

[Close]

BLAST of CmoCh06G014440 vs. ExPASy Swiss-Prot
Match: Q10M74 (Guanylate kinase 2, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=V2 PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.2e-88
Identity = 176/267 (65.92%), Postives = 213/267 (79.78%), Query Frame = 0


Query: 32  PKSIKSKPIQPFPRTP---TFFSSSSKMGDARRPLSANIPPPDKAERSELLRSLEFSLNS 91
           P+++ + P  P PR P      SSSS    + RP     PPP  A++ +L R LE +L +
Sbjct: 24  PRAVPATP--PAPRPPPRRLMSSSSSGWHHSSRPPP---PPPSGADKDQLFRGLEAALGT 83

Query: 92  GFSSEPLLPKPSPLIIVISGPSGVGKDAVIKRLREVREGLRFVVTATSRSMRPGEVDGED 151
            FSSEPL P P P+I+VISGPSGVGKDAVI+RL+E REG+ FVVTATSR+ RPGEVDG+D
Sbjct: 84  TFSSEPLAPPPQPMILVISGPSGVGKDAVIQRLQEEREGMHFVVTATSRAKRPGEVDGKD 143

Query: 152 YYFVSKEEFLAMIERNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRK 211
           YYFV+KEEFL MIER ELLEYALVYG+YKGIPK+QIR++MA+G+DIVLRVDIQGA TLR+
Sbjct: 144 YYFVTKEEFLTMIERKELLEYALVYGEYKGIPKQQIRDYMAKGYDIVLRVDIQGAATLRE 203

Query: 212 ILGKSAVFVFLMAESELKLVERLIDRKTETKESLLVRVATAREEVRHVKNFDYVVVNAEG 271
           ILG+SA+F+FL+AESE  LV+RLI RKTET + LLVRVATAREEV+ + NFDYVVVN+EG
Sbjct: 204 ILGESAIFIFLVAESEEALVKRLIHRKTETSDMLLVRVATAREEVKRMNNFDYVVVNSEG 263

Query: 272 KLENAAKLVGSIIDAEKAKVWQRNASV 296
            LE A K V SIIDAEKAKV +R  ++
Sbjct: 264 NLEGAVKQVESIIDAEKAKVHKRTVNI 285

[Close]

BLAST of CmoCh06G014440 vs. ExPASy Swiss-Prot
Match: Q3ZAF9 (Guanylate kinase OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) OX=243164 GN=gmk PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.6e-43
Identity = 92/192 (47.92%), Postives = 137/192 (71.35%), Query Frame = 0


Query: 101 PLIIVISGPSGVGKDAVIKRLREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAM 160
           PL++V+SGPSGVGKDAV+ R++E +  L ++VT T+R+ R  E +G DY F+   EF  +
Sbjct: 13  PLLLVVSGPSGVGKDAVLARMKERKLPLAYIVTTTTRTKREKETEGVDYNFIRPAEFQQL 72

Query: 161 IERNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLM 220
           I +NELLE+A VYG++ G+PK  IR+ +A GFD++++VD+QGA +++KI+  +AVF+FLM
Sbjct: 73  IGQNELLEWANVYGNFYGVPKAPIRQALAHGFDVIVKVDVQGAASIKKIV-PNAVFIFLM 132

Query: 221 AESELKLVERLIDRKTETKESLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSI 280
                +L  RL  R TE+ ESL  R+ATA  E+  + +FDYVVVN EG+++NA   + SI
Sbjct: 133 PPDMDELTRRLEHRLTESPESLKRRLATAPLEIEKLPDFDYVVVNPEGEIDNAVSEIMSI 192

Query: 281 IDAEKAKVWQRN 293
           I AE  ++  R+
Sbjct: 193 ISAEHCRINPRS 203

[Close]

BLAST of CmoCh06G014440 vs. ExPASy Swiss-Prot
Match: Q3ZWD8 (Guanylate kinase OS=Dehalococcoides mccartyi (strain CBDB1) OX=255470 GN=gmk PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.1e-42
Identity = 89/191 (46.60%), Postives = 136/191 (71.20%), Query Frame = 0


Query: 101 PLIIVISGPSGVGKDAVIKRLREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAM 160
           PL++++SGPSGVGKDAV+ R++E +  L ++VT T+R  R  E +G DY F+   EF  +
Sbjct: 13  PLLLIVSGPSGVGKDAVLARMKERKLPLTYIVTTTTRPKRETETEGVDYNFIRPAEFQEL 72

Query: 161 IERNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLM 220
           I +NELLE+A VYG++ G+PK  IR+ +++G D++++VD+QGA +++KI+  +AVF+FLM
Sbjct: 73  ISQNELLEWANVYGNFYGVPKAPIRQALSRGSDVIVKVDVQGAASIKKIV-PNAVFIFLM 132

Query: 221 AESELKLVERLIDRKTETKESLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSI 280
                +L  RL  R TE+ ESL  R+ATA  E+  + +FDYVVVN EG+++NA + + SI
Sbjct: 133 PPDMDELTRRLEHRLTESPESLRRRLATAPLEIEKLPDFDYVVVNPEGEIDNAVREIMSI 192

Query: 281 IDAEKAKVWQR 292
           I AE  ++  R
Sbjct: 193 IAAEHCRIQPR 202

[Close]

BLAST of CmoCh06G014440 vs. ExPASy Swiss-Prot
Match: Q895Q5 (Guanylate kinase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=gmk PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.1e-35
Identity = 83/187 (44.39%), Postives = 122/187 (65.24%), Query Frame = 0


Query: 102 LIIVISGPSGVGKDAVIKRLREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMI 161
           L+IVISGPSG GK  V K L        F V++T+R  R GE+ G+ YYF+SKEEF   I
Sbjct: 5   LLIVISGPSGTGKGTVCKELLR-NNNFWFSVSSTTRDPREGEIQGKSYYFMSKEEFEDKI 64

Query: 162 ERNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMA 221
           + N+ LEYA VYG+Y G PK ++ E + +G D++L +DIQGA  +++   K  +F+F++ 
Sbjct: 65  KENDFLEYAKVYGNYYGTPKSKVIEMLDKGKDVILEIDIQGALQVKENY-KEGIFIFILP 124

Query: 222 ESELKLVERLIDRKTETKESLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSII 281
            S  +L  R+I R TET+ESL+ R  +A +E+ +V  ++Y VVN   K+ +A + + SII
Sbjct: 125 PSMEELKNRIIKRGTETEESLMTRFKSAYKEINYVSKYNYAVVN--DKVHDAVEKIQSII 184

Query: 282 DAEKAKV 289
            AEK +V
Sbjct: 185 SAEKCRV 187

[Close]

BLAST of CmoCh06G014440 vs. ExPASy TrEMBL
Match: A0A6J1GHH3 (Guanylate kinase OS=Cucurbita moschata OX=3662 GN=LOC111454231 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.5e-158
Identity = 295/295 (100.00%), Postives = 295/295 (100.00%), Query Frame = 0


Query: 1   MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR 60
           MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR
Sbjct: 1   MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR 60

Query: 61  RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR 120
           RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR
Sbjct: 61  RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR 120

Query: 121 LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP 180
           LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP
Sbjct: 121 LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP 180

Query: 181 KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE 240
           KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE
Sbjct: 181 KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE 240

Query: 241 SLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV 296
           SLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV
Sbjct: 241 SLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV 295

[Close]

BLAST of CmoCh06G014440 vs. ExPASy TrEMBL
Match: A0A6J1I8F5 (Guanylate kinase OS=Cucurbita maxima OX=3661 GN=LOC111472329 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 8.1e-155
Identity = 290/295 (98.31%), Postives = 290/295 (98.31%), Query Frame = 0


Query: 1   MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR 60
           MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKS KSKPIQPFPR PTFFSSSSKMGDAR
Sbjct: 1   MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSPKSKPIQPFPRNPTFFSSSSKMGDAR 60

Query: 61  RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR 120
           RPLSANIPPPDKAER ELLRSLEFSLNS FSSEPLLPKPSPLIIVISGPSGVGKDAVIKR
Sbjct: 61  RPLSANIPPPDKAERLELLRSLEFSLNSSFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR 120

Query: 121 LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP 180
           LREVREGL FVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP
Sbjct: 121 LREVREGLHFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP 180

Query: 181 KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE 240
           KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE
Sbjct: 181 KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE 240

Query: 241 SLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV 296
           SLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV
Sbjct: 241 SLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV 295

[Close]

BLAST of CmoCh06G014440 vs. ExPASy TrEMBL
Match: A0A6J1GIP0 (guanylate kinase 3, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454231 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.4e-129
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR 60
           MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR
Sbjct: 1   MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR 60

Query: 61  RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR 120
           RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR
Sbjct: 61  RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR 120

Query: 121 LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP 180
           LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP
Sbjct: 121 LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP 180

Query: 181 KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE 240
           KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE
Sbjct: 181 KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE 240

Query: 241 SLL 244
           SLL
Sbjct: 241 SLL 243

[Close]

BLAST of CmoCh06G014440 vs. ExPASy TrEMBL
Match: A0A6J1INA9 (Guanylate kinase OS=Cucurbita maxima OX=3661 GN=LOC111479039 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.5e-129
Identity = 250/295 (84.75%), Postives = 265/295 (89.83%), Query Frame = 0


Query: 1   MSCRFFGAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGDAR 60
           MS RFFGA LSRS  L  KFHNP SL NF      KSKPIQ FP TP  FSS S+MGDAR
Sbjct: 1   MSSRFFGASLSRSSTLHPKFHNPNSLPNF-----PKSKPIQSFPLTPLSFSSISEMGDAR 60

Query: 61  RPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKR 120
           RP+SA IPPPDKA+RSELLRSLEFSL S FSSEPL+P PSPLIIVISGPSGVGKDAVIKR
Sbjct: 61  RPISATIPPPDKADRSELLRSLEFSLGSSFSSEPLVPNPSPLIIVISGPSGVGKDAVIKR 120

Query: 121 LREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIP 180
           LREVREGL FVVTATSR +RPGEVDG+DYYFVSKEEFLA+IERNELLEYALVYGDYKGIP
Sbjct: 121 LREVREGLHFVVTATSRPIRPGEVDGKDYYFVSKEEFLALIERNELLEYALVYGDYKGIP 180

Query: 181 KRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKE 240
           KRQIR+FMA+G+DIVLRVDIQGAETLRKILG SAVFVFLMAE+ELKLVERLI+RKTETKE
Sbjct: 181 KRQIRDFMAKGYDIVLRVDIQGAETLRKILGNSAVFVFLMAENELKLVERLINRKTETKE 240

Query: 241 SLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV 296
           SL+VRVATAREEV+HV NFDYVVVNAEGKLE+A KLV SIIDAEKAKVWQRNA V
Sbjct: 241 SLMVRVATAREEVKHVNNFDYVVVNAEGKLESAVKLVESIIDAEKAKVWQRNAVV 290

[Close]

BLAST of CmoCh06G014440 vs. ExPASy TrEMBL
Match: A0A6J1C9N3 (Guanylate kinase OS=Momordica charantia OX=3673 GN=LOC111009480 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.0e-128
Identity = 250/297 (84.18%), Postives = 267/297 (89.90%), Query Frame = 0


Query: 1   MSCRFF--GAYLSRSGNLRIKFHNPISLTNFLNPKSIKSKPIQPFPRTPTFFSSSSKMGD 60
           MS RFF  GA LSRS  L  KF NPISL  FL+P   KSK I PFP TP FFSS+S+MGD
Sbjct: 1   MSRRFFVGGASLSRSSILHAKFRNPISLPYFLHPNFPKSKSIHPFPITPRFFSSNSQMGD 60

Query: 61  ARRPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVI 120
           ARRPLSANIPPP+ A+RSELLRSLEFSLNS FSSEPLLP PSPLIIVISGPSGVGKDAVI
Sbjct: 61  ARRPLSANIPPPETADRSELLRSLEFSLNSSFSSEPLLPNPSPLIIVISGPSGVGKDAVI 120

Query: 121 KRLREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKG 180
           KRLREVREGL FVVTATSR MRPGEVDG DYYFVSKEEFLAMIERNELLEYALVYGDYKG
Sbjct: 121 KRLREVREGLHFVVTATSRPMRPGEVDGVDYYFVSKEEFLAMIERNELLEYALVYGDYKG 180

Query: 181 IPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTET 240
           IPK+QIREFMA+G DIVLRVDIQGAETL+KILG +AVF+FLMAESE+KLVERLI+RKTET
Sbjct: 181 IPKKQIREFMARGHDIVLRVDIQGAETLKKILGNTAVFLFLMAESEIKLVERLINRKTET 240

Query: 241 KESLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSIIDAEKAKVWQRNASV 296
           KESLLVRVATAREEV+HVK+FDYVVVN EG+LE+A KLV SIIDAEKAKVWQRNA +
Sbjct: 241 KESLLVRVATAREEVKHVKSFDYVVVNTEGELESAVKLVESIIDAEKAKVWQRNAVI 297

[Close]

BLAST of CmoCh06G014440 vs. TAIR 10
Match: AT3G06200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.6e-91
Identity = 172/251 (68.53%), Postives = 208/251 (82.87%), Query Frame = 0


Query: 41  QPFPRTPTFFSSSSKMGDARRPLSANIPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPS 100
           QP   +     SS KM D   P + N  P     + E LRSLEF L S F+++P++P P+
Sbjct: 28  QPLRHSSALSYSSLKMTDTHIPGNPNSSPIQSPTKPESLRSLEFQLGSSFTADPIIPPPN 87

Query: 101 PLIIVISGPSGVGKDAVIKRLREVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAM 160
            ++IVISGPSGVGKDAVI +LREVREGL FVVTATSR MRPGEVDG+DY+FVS+++FL+M
Sbjct: 88  QIVIVISGPSGVGKDAVINKLREVREGLHFVVTATSRPMRPGEVDGKDYFFVSRDQFLSM 147

Query: 161 IERNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLM 220
           +E  ELLEYALVYG+YKGIPK+QI+EFMA+G DIVLRVDIQGA+TLR+ILG SAVF+FL+
Sbjct: 148 VENEELLEYALVYGEYKGIPKKQIQEFMAKGEDIVLRVDIQGAQTLRRILGNSAVFIFLV 207

Query: 221 AESELKLVERLIDRKTETKESLLVRVATAREEVRHVKNFDYVVVNAEGKLENAAKLVGSI 280
           AESEL +VERLIDRKTE++E LLVRVATAREEVRH+KNFDYVVVNA+G+L++A   V SI
Sbjct: 208 AESELAMVERLIDRKTESQEELLVRVATAREEVRHLKNFDYVVVNAKGRLDDAVNRVESI 267

Query: 281 IDAEKAKVWQR 292
           IDAEK+KV QR
Sbjct: 268 IDAEKSKVHQR 278

[Close]

BLAST of CmoCh06G014440 vs. TAIR 10
Match: AT2G41880.1 (guanylate kinase 1 )

HSP 1 Score: 86.7 bits (213), Expect = 3.5e-17
Identity = 62/189 (32.80%), Postives = 95/189 (50.26%), Query Frame = 0


Query: 67  IPPPDKAERSELLRSLEFSLNSGFSSEPLLPKPSPLIIVISGPSGVGKDAVIKRL-REVR 126
           +  P   E+ +LLR    + + G       P      IVISGPSGVGK  +I  L +E  
Sbjct: 109 VDSPYVREQKKLLRKEVVAWSKGVRGNAEKP------IVISGPSGVGKGTLISMLMKEFP 168

Query: 127 EGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIERNELLEYALVYGDYKGIPKRQIR 186
               F V+ T+RS R  E+DG  Y+F  K+     I+  + LE+A V+G+  G     + 
Sbjct: 169 SMFGFSVSHTTRSPRSMEMDGVHYHFADKKVMEKEIKDGKFLEFASVHGNLYGTSIESVE 228

Query: 187 EFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAESELKLVERLIDRKTETKESLLVR 246
                G   +L +D+QGA ++R      A+F+F+   S  +L +RL  R TET+E +  R
Sbjct: 229 AVTDSGKRCILDIDVQGARSVR-ASSLDAIFIFVCPPSMKELEDRLRARGTETEEQIQKR 288

Query: 247 VATAREEVR 255
           +  A  E++
Sbjct: 289 LRNAEAEIK 290

[Close]

BLAST of CmoCh06G014440 vs. TAIR 10
Match: AT3G57550.1 (guanylate kinase )

HSP 1 Score: 79.0 bits (193), Expect = 7.3e-15
Identity = 59/182 (32.42%), Postives = 95/182 (52.20%), Query Frame = 0


Query: 104 IVISGPSGVGKDAVIKRL-REVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIE 163
           IVISGPSGVGK  +I  L +E      F V+ T+R+ R  E +G  Y+F  K      I+
Sbjct: 140 IVISGPSGVGKGTLISMLMKEFPSMFGFSVSHTTRAPRCMEKNGVHYHFTDKTVMEKEIK 199

Query: 164 RNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAE 223
             + LE+A V+G+  G     +      G   +L +D+QGA ++ K     A+F+F+   
Sbjct: 200 DGKFLEFASVHGNLYGTSIESVEVVTDSGKRCILDIDVQGARSV-KASSLDAIFIFVCPP 259

Query: 224 SELKLVERLIDRKTETKESLLVRVATAREEVRHVKN---FDYVVVNAEGKLENAAKLVGS 282
           S  +L +RL  R TET+E +  R+  A  E++  K+   F++ + N    LE   K + +
Sbjct: 260 SMKELEDRLRARGTETEEQIQKRLRNADAEIKAGKSSGIFEHKLYN--DNLEECYKTLKN 318

[Close]

BLAST of CmoCh06G014440 vs. TAIR 10
Match: AT3G57550.2 (guanylate kinase )

HSP 1 Score: 79.0 bits (193), Expect = 7.3e-15
Identity = 59/182 (32.42%), Postives = 95/182 (52.20%), Query Frame = 0


Query: 104 IVISGPSGVGKDAVIKRL-REVREGLRFVVTATSRSMRPGEVDGEDYYFVSKEEFLAMIE 163
           IVISGPSGVGK  +I  L +E      F V+ T+R+ R  E +G  Y+F  K      I+
Sbjct: 140 IVISGPSGVGKGTLISMLMKEFPSMFGFSVSHTTRAPRCMEKNGVHYHFTDKTVMEKEIK 199

Query: 164 RNELLEYALVYGDYKGIPKRQIREFMAQGFDIVLRVDIQGAETLRKILGKSAVFVFLMAE 223
             + LE+A V+G+  G     +      G   +L +D+QGA ++ K     A+F+F+   
Sbjct: 200 DGKFLEFASVHGNLYGTSIESVEVVTDSGKRCILDIDVQGARSV-KASSLDAIFIFVCPP 259

Query: 224 SELKLVERLIDRKTETKESLLVRVATAREEVRHVKN---FDYVVVNAEGKLENAAKLVGS 282
           S  +L +RL  R TET+E +  R+  A  E++  K+   F++ + N    LE   K + +
Sbjct: 260 SMKELEDRLRARGTETEEQIQKRLRNADAEIKAGKSSGIFEHKLYN--DNLEECYKTLKN 318

The following BLAST results are available for this feature:

BLAST of CmoCh06G014440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94JM2	2.3e-90	68.53	Guanylate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GK3 PE=2 SV...	[more]
Q10M74	2.2e-88	65.92	Guanylate kinase 2, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica ...	[more]
Q3ZAF9	1.6e-43	47.92	Guanylate kinase OS=Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 ...	[more]
Q3ZWD8	3.1e-42	46.60	Guanylate kinase OS=Dehalococcoides mccartyi (strain CBDB1) OX=255470 GN=gmk PE=...	[more]
Q895Q5	2.1e-35	44.39	Guanylate kinase OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN...	[more]

BLAST of CmoCh06G014440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GHH3	3.5e-158	100.00	Guanylate kinase OS=Cucurbita moschata OX=3662 GN=LOC111454231 PE=3 SV=1	[more]
A0A6J1I8F5	8.1e-155	98.31	Guanylate kinase OS=Cucurbita maxima OX=3661 GN=LOC111472329 PE=3 SV=1	[more]
A0A6J1GIP0	3.4e-129	100.00	guanylate kinase 3, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1INA9	4.5e-129	84.75	Guanylate kinase OS=Cucurbita maxima OX=3661 GN=LOC111479039 PE=3 SV=1	[more]
A0A6J1C9N3	1.0e-128	84.18	Guanylate kinase OS=Momordica charantia OX=3673 GN=LOC111009480 PE=3 SV=1	[more]

BLAST of CmoCh06G014440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06200.1	1.6e-91	68.53	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G41880.1	3.5e-17	32.80	guanylate kinase 1	[more]
AT3G57550.1	7.3e-15	32.42	guanylate kinase	[more]
AT3G57550.2	7.3e-15	32.42	guanylate kinase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 232..252
None	No IPR available	GENE3D	3.30.63.10	Guanylate Kinase phosphate binding domain	coord: 132..193 e-value: 1.1E-52 score: 180.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 51..71
None	No IPR available	PANTHER	PTHR23117	GUANYLATE KINASE-RELATED	coord: 53..293
None	No IPR available	PANTHER	PTHR23117:SF13	GUANYLATE KINASE	coord: 53..293
None	No IPR available	CDD	cd00071	GMPK	coord: 103..220 e-value: 1.98081E-51 score: 163.857
IPR008145	Guanylate kinase/L-type calcium channel beta subunit	SMART	SM00072	gk_7	coord: 100..285 e-value: 4.2E-40 score: 149.2
IPR008145	Guanylate kinase/L-type calcium channel beta subunit	PFAM	PF00625	Guanylate_kin	coord: 104..283 e-value: 1.2E-38 score: 132.6
IPR017665	Guanylate kinase	TIGRFAM	TIGR03263	TIGR03263	coord: 103..282 e-value: 2.5E-55 score: 184.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 103..273 e-value: 1.1E-52 score: 180.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 99..288
IPR020590	Guanylate kinase, conserved site	PROSITE	PS00856	GUANYLATE_KINASE_1	coord: 135..152
IPR008144	Guanylate kinase-like domain	PROSITE	PS50052	GUANYLATE_KINASE_2	coord: 101..282 score: 30.090475

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G014440.1	CmoCh06G014440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046710	GDP metabolic process
biological_process	GO:0046037	GMP metabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0006163	purine nucleotide metabolic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004385	guanylate kinase activity