CmoCh06G004890 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G004890
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme 1-like
LocationCmo_Chr06: 2364032 .. 2369324 (+)
RNA-Seq ExpressionCmoCh06G004890
SyntenyCmoCh06G004890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAAAAGAAAAAATTATCCTCTCTCTCTCTCTATCTCTACAAATTCTCGCTCTATAAATAACCATCGAAACTCCTGCTTAGATGTTCAACTTCAAACTCCTCACCGGAGGACTTTCAGCGTTAGGTTTTAAATTCCTCAATGAATTTCTTCACTAAAATCCTCTCTCTTCTTCTCGGATTCGCCTTTTTTTCTCTGTTCATCAACAATGGCGTAGTCTTCGTCCACGCTCTCCCCAAATTCTATCCGGAATTACAGTCTCTTAACAGAGCGGACGATGCCGTGATCAAGCCGCTCCATCGAACTCGTTTCCACTTCCAGCCTCGGCGCCACTGGATCAACGGTTCGTTATTCTTCCATAATTTCACTCTGTTTCGCTTTAATCTCTGTTCTGCGGATGTTTTGATTTGATTTGATTTGGTTCATGCGTCCGTGACTGTGAGTTCCGACTTTCGTAAAGCGTTTTGGTAATCGATAACTTTCATGATTCGAGTGCGGACTTGGATTCGCATTATCTCATTCCGTTACTGTTTTCATTTTCATTTTTTTATAATCGAAGATATTGTATATTTGTTTTTGCTCTCTTGCCGTCTGATTGGTGGTTGCTTCTAGGGACTCTATCAATTCTAACCGTCTGATCAGTTTTTTTCCCTACTTTTGGTATCCTTATCATCATTGCTGCGTACTGTTCTTACCTTATAATAATTATTATTTTTTTCTAACTCTTAGAGATTACTATTTAACTAGTGGGTCCGACTTCCAACTTATCTCGACTAGAAATTGAGAGATTTTTCTTTTCTCCAAGAAATTAGAGATGAATGATATACTTCAAGATTAGCGTTTTTATTATTATTATTGAAAAGAAATTCTAATCATGTGTATATCGTTTCAAGTCTAATAAGTTTTTTTTTATAAAGATTAAGATTGAAATAAGAGTTGGAGACCAGGAAGGTTTCTTTGTCCTGTGCACACCTCTAAGGTCATGTGCAAATCGCCCAATTTTTTTTTTTTTTTGGTAAGTGGTTGGATGTACAATATTATTCTTTATAAACACTTTTGGATGACTTAAGAAAAATCGAGCATATTCTAGAGTAAAAAATGAACTCCAAGCATCCGAACATTTTTTTCCTTTTTGTTTTTATGTCTTTTCTAGTTTAAATATAATCTAGGAACATAAACTTGAAAAAAAAAAGTACTTAAATATGTAAGTTTAATCTTCAAATTAAAATTTAATATCCAACAAATCTATAAAATTAAAAAAAAAAAAAAAATGTAGATGTATATAGAAACACAGGGAAAGTGAGATTTGACATGGTGATATTGTGACATAGGATACACGTGAGCACTTTAATTTTTCTAAAAATATGTTAATGGTGGGGCCCATAAACTCAATATGTTATTGGTTGTATTTTTATTTTTCCTTATTTATTACATGGCATTCAGTGAGTGGGGAATATTTTATCCTTCTATTTTAATTTTATACTTTCTAGAGATTATTTTTTAAATTTATGAAAAATATAAAAATTAAATATATATATATATTAGAAGACCAAATAGCTTTTTTAGTGAACTGAAGTGTGGTTATAATATTATATTTAAAAAGTAAGGATAGAGGTAAAATAATATAGTATTATTCAATTTGATGAGTTTTCTTAAAAATATCGAAACTATTATTAAAAACTGGACTTCTACAAGTGGCTGGGAATCTCTAATGATAATAATAATAATATTTATATATATATATATAGAAAACTGAAGTGGAGATTTGCTCTTCCTTTTTGTAGAAAAAGCTTATTGGGTCAAAATTATTTAATTTTGGTATGCAAATGTTTACAATAACCCTATTTTGTATGTAGGGGTCAGCTGGGTTTCCAAATAAAACAAATGCTTGTTGTTCCTACTAATCGTTTAAATTTATTACATGATTTGAGAAAAAGTGGTTTTATTATGAATTTTTATAATGATATGATATTGTCTATTTTATTCCCATAATATTCTTTATAAAATTATAATTTTTTACTTTAAACCTTTTGGTATCCTAAAGAAAAGTTTTATTTTCTTTTTAAAATAGGGATTAAATGTTTTACGTTAAAGATTTCGATGGTTTGTGTGTTTTTTGGGTAACTAATATGGTTTGGTTGTGGTTCGTATATGTGCGGTGGTGCTCAAATCCTGTCAACAACTTAACCAAATGCAGACCCAAATGGTGAGTTATTGCAATACACCTAAGCTTATTTTATTTACTTTTTAATAATAATAATAATAAAAGAAACTTGTTTCTTCTTCGTAATGTTTTTTTTTTTTTAATTATTTCTTAAATATGTTTTATATATAAATAAATGATTTCTTTTATAAGAAAAAGGTTAAGATTTGAAAAAGAAAAGAATATTAAACTAAAAAGTTGGAAAATAAAGGTGTAGATAAATTATCCCAAGACTAATAATAAAAACTTTTAGAGTTTTTTATTTTTGAAAATTTGATGATATTTTTTTAAATGTGGTATAAGGTATTTTTTAGCTTTTAAAATAAATTTTAAAAAAATTTAGTAGTTTTTTCTTTTTAAAATTGAGAATATTAAAAAAAAAATTAAAATTTCACGGAAATTTGTAAACATATTTGTATACAAAATAAAAGGTTTAATAATATTTTTATTAATTTAACAGAAAAAGTAAGCTTTTAGTTTTGCATATCCTATTTAAAATTTAGAAAATGGGATCACTTTTGATTTTTCAAAAATTTCATTAATACCCCTTAAAAAAAATTAAAAAAATATAATGTTACCATAAATATCGCGTTTCAAAAATACTATTAGCTTTTCCAACTAAAATTAAAAATGAGATGTGCAGTCGTAAAAACTTTTAAAAACATTTTTTTTAAAGGGGAAAGATGTTAATACTACATTTAAAAATTTATGGATATTTTTTAAATATATTACTAATATAATAATATATTTTAAATGAAAAGTGTTAATAAAACTTTAAGGATACTAAACAAACAACTAATAATTTTTATCAAAAAAAATTTTAAAAGTTATTTTTTGAGACCAAATAAAATTTGTTAAGAGTATATTTATGAATTTAGCCATAAAAAAAAAAAAAAAAAAAAAAGAAATTAATGGTGGTGTGAATTACTGTTTTGACCAAGCGTATTGTGAACGATGAAACAGGCCCAATGTACTTCAATGGAATTTACCACCTCTTCTACCAGTACAACCCCAAAGGCGCAATTTGGGGCAACATAGTTTGGGCTCATTCGGTGTCCAGAGACCTGGTCAATTGGAAGGCACTTAAACACGCCATTTACCCTTCCAAGCCCTTCGACATCAATGGCTGTTGGTCCGGCTCCGCCACTGTCCTCCCGGGCAACAAGCCCGTCATTCTATACACCGGAGTCGACCCCCAAAACCGCCAGGTTCAAAACTACGCCATTCCGGCCAACCTCTCCGACCCCTATCTCACCGACTGGATCAAACCCGACGACAACCCGATCGTCGACCCGGATTCGGGAGTCAACGCCAGCGCCTTCCGGGACCCAACGACGGCGTGGCTGAGCAACAGCGGACACTGGAAAACCATCATCGGTAGCAAGAGACGGAAGAGGGGAGTGGCGTATTTATACAGAAGTAAGGATTTCGTGAAATGGACAAAGGCGAAACACCCTTTACACTCTGCCCCTAATACAGGCATGTGGGAGTGCCCTGATTTCTTCCCGGTTCCTCTGTCGGGCAAGCAGGGATTGGACGTGTCGGTGACGGGGAATTGGGTTAAACATGTGCTGAAGGTGAGTTTGGATGTTACTAGATATGAGTATTACACGGTGGGGAAGTATTATCATGAGCGAGATAGGTATGTTCCCGACAATACATCGGTGGACGGATGGAGCGGGCTGAGATATGACTATGGGAATTTCTATGCCTCAAAGACGTTTTATGATCCGGCGAAGAAGAGGAGGGTTTTGTGGGGTTGGGCTAATGAGTCTGATAGTGCTGTGGATGATATTTCTAAAGGATGGGCTGGAATTCAGGTCTGTTTTTTCCTTGTTGGTTTGGATTGCTTGTGAATGTCTGACCCTCCTGAGGCCTTTGAACGATTGTTTTTGCAGGCAATTCCAAGGACGGTGTGGTTGGATCCTAATAGGAAGCAACTTTTGCAATGGCCTGTTGAGGAGCTGAATGGGCTGAGAGGGAAGCAAGTTAAGATGAGTCATCAGAAGCTGTACAAGGAACAACATGTTGAGGTTAAAGGAATTACTGCTGCCCAGGTCTGTCGGATTTCAATGGTGTTATGATTTTGTTTGCAGTTTGTTTCTAACATATATTTGTACAGGCTGATGTCGAAGTTACGTTCAGCTTTTCAAGCTTGGATAAAGCTGAGCCGTTTGATCCAAGCTGGGTGGATGCACAAGCAGTGTGTGGTAAACTGGGCTCAAAGGTCCAAGGTGGAGTTGGCCCGTTTGGGCTTCTGACACTGGCATCAGAGAACCTCCAGGAATTCACTCCTGTGTTCTTCAGGATTTTCAAGGCCCATCACAATCATGTGGTTCTTCTGTGCTCTGATGCAACAAGGTAAACTCCTCTCATCAAACAAAATAGTTATTAAACCAGAACAAGACTTCAATTATTGACTCTGTTTTGTGGTGTAGCTCGTCTCTGAAGAAGGATGGATTGTACAAGCCATCATTTGCAGGGTTCGTGGACATCGACTTATCGAGCAAAAAGCTCCCATTAAGAACTCTGGTATGTAAAAACCATAGCCAAATGTTTTTGTCCCCCAAAAGTTTAAAATCTAAACCGTAATGATATGATATGGAATGTTTCTTGATATATAGATTGATCACTCGGTGGTGGAGAGTTTTGGAGGAGGTGGAAAAACGTGCATAACTTCAAGGGTATATCCAACAAAGGCGGTGTTTGGAGATGCACATTTGTATGTGTTCAACAATGGAAGCGAGGCTATCACAGTAGACTATCTAAGTGCTTGGAGTATGAGGAGTGCTACGATCAACTAAGTCTTTGTAAGTTATATAACTTAGAATGTTGTTTTTGCTTTAGTTTACTAAATGTTTAGTATTTTAGAGAGGTGGGTTATTTTAGGACATAATTAAGGTTGTGTAATAGGAGATGGCTGACCTCTCCTTTGTCTTTGATGTTTCTGGTTTAAGAGTTGATCTTCACTCTTATGTAGAACTTTAATAATCGACATTCAATGTGGATGATATAAAATGTTCTCCACTTGTTTTCTATAAAAACTTTAGCAATCAAATGATCAATTTTATTGTGAATTGCATCACGAATA

mRNA sequence

AAGAAAAGAAAAAATTATCCTCTCTCTCTCTCTATCTCTACAAATTCTCGCTCTATAAATAACCATCGAAACTCCTGCTTAGATGTTCAACTTCAAACTCCTCACCGGAGGACTTTCAGCGTTAGGTTTTAAATTCCTCAATGAATTTCTTCACTAAAATCCTCTCTCTTCTTCTCGGATTCGCCTTTTTTTCTCTGTTCATCAACAATGGCGTAGTCTTCGTCCACGCTCTCCCCAAATTCTATCCGGAATTACAGTCTCTTAACAGAGCGGACGATGCCGTGATCAAGCCGCTCCATCGAACTCGTTTCCACTTCCAGCCTCGGCGCCACTGGATCAACGACCCAAATGGCCCAATGTACTTCAATGGAATTTACCACCTCTTCTACCAGTACAACCCCAAAGGCGCAATTTGGGGCAACATAGTTTGGGCTCATTCGGTGTCCAGAGACCTGGTCAATTGGAAGGCACTTAAACACGCCATTTACCCTTCCAAGCCCTTCGACATCAATGGCTGTTGGTCCGGCTCCGCCACTGTCCTCCCGGGCAACAAGCCCGTCATTCTATACACCGGAGTCGACCCCCAAAACCGCCAGGTTCAAAACTACGCCATTCCGGCCAACCTCTCCGACCCCTATCTCACCGACTGGATCAAACCCGACGACAACCCGATCGTCGACCCGGATTCGGGAGTCAACGCCAGCGCCTTCCGGGACCCAACGACGGCGTGGCTGAGCAACAGCGGACACTGGAAAACCATCATCGGTAGCAAGAGACGGAAGAGGGGAGTGGCGTATTTATACAGAAGTAAGGATTTCGTGAAATGGACAAAGGCGAAACACCCTTTACACTCTGCCCCTAATACAGGCATGTGGGAGTGCCCTGATTTCTTCCCGGTTCCTCTGTCGGGCAAGCAGGGATTGGACGTGTCGGTGACGGGGAATTGGGTTAAACATGTGCTGAAGGTGAGTTTGGATGTTACTAGATATGAGTATTACACGGTGGGGAAGTATTATCATGAGCGAGATAGGTATGTTCCCGACAATACATCGGTGGACGGATGGAGCGGGCTGAGATATGACTATGGGAATTTCTATGCCTCAAAGACGTTTTATGATCCGGCGAAGAAGAGGAGGGTTTTGTGGGGTTGGGCTAATGAGTCTGATAGTGCTGTGGATGATATTTCTAAAGGATGGGCTGGAATTCAGGCAATTCCAAGGACGGTGTGGTTGGATCCTAATAGGAAGCAACTTTTGCAATGGCCTGTTGAGGAGCTGAATGGGCTGAGAGGGAAGCAAGTTAAGATGAGTCATCAGAAGCTGTACAAGGAACAACATGTTGAGGTTAAAGGAATTACTGCTGCCCAGGCTGATGTCGAAGTTACGTTCAGCTTTTCAAGCTTGGATAAAGCTGAGCCGTTTGATCCAAGCTGGGTGGATGCACAAGCAGTGTGTGGTAAACTGGGCTCAAAGGTCCAAGGTGGAGTTGGCCCGTTTGGGCTTCTGACACTGGCATCAGAGAACCTCCAGGAATTCACTCCTGTGTTCTTCAGGATTTTCAAGGCCCATCACAATCATGTGGTTCTTCTGTGCTCTGATGCAACAAGCTCGTCTCTGAAGAAGGATGGATTGTACAAGCCATCATTTGCAGGGTTCGTGGACATCGACTTATCGAGCAAAAAGCTCCCATTAAGAACTCTGATTGATCACTCGGTGGTGGAGAGTTTTGGAGGAGGTGGAAAAACGTGCATAACTTCAAGGGTATATCCAACAAAGGCGGTGTTTGGAGATGCACATTTGTATGTGTTCAACAATGGAAGCGAGGCTATCACAGTAGACTATCTAAGTGCTTGGAGTATGAGGAGTGCTACGATCAACTAAGTCTTTGTAAGTTATATAACTTAGAATGTTGTTTTTGCTTTAGTTTACTAAATGTTTAGTATTTTAGAGAGGTGGGTTATTTTAGGACATAATTAAGGTTGTGTAATAGGAGATGGCTGACCTCTCCTTTGTCTTTGATGTTTCTGGTTTAAGAGTTGATCTTCACTCTTATGTAGAACTTTAATAATCGACATTCAATGTGGATGATATAAAATGTTCTCCACTTGTTTTCTATAAAAACTTTAGCAATCAAATGATCAATTTTATTGTGAATTGCATCACGAATA

Coding sequence (CDS)

ATGAATTTCTTCACTAAAATCCTCTCTCTTCTTCTCGGATTCGCCTTTTTTTCTCTGTTCATCAACAATGGCGTAGTCTTCGTCCACGCTCTCCCCAAATTCTATCCGGAATTACAGTCTCTTAACAGAGCGGACGATGCCGTGATCAAGCCGCTCCATCGAACTCGTTTCCACTTCCAGCCTCGGCGCCACTGGATCAACGACCCAAATGGCCCAATGTACTTCAATGGAATTTACCACCTCTTCTACCAGTACAACCCCAAAGGCGCAATTTGGGGCAACATAGTTTGGGCTCATTCGGTGTCCAGAGACCTGGTCAATTGGAAGGCACTTAAACACGCCATTTACCCTTCCAAGCCCTTCGACATCAATGGCTGTTGGTCCGGCTCCGCCACTGTCCTCCCGGGCAACAAGCCCGTCATTCTATACACCGGAGTCGACCCCCAAAACCGCCAGGTTCAAAACTACGCCATTCCGGCCAACCTCTCCGACCCCTATCTCACCGACTGGATCAAACCCGACGACAACCCGATCGTCGACCCGGATTCGGGAGTCAACGCCAGCGCCTTCCGGGACCCAACGACGGCGTGGCTGAGCAACAGCGGACACTGGAAAACCATCATCGGTAGCAAGAGACGGAAGAGGGGAGTGGCGTATTTATACAGAAGTAAGGATTTCGTGAAATGGACAAAGGCGAAACACCCTTTACACTCTGCCCCTAATACAGGCATGTGGGAGTGCCCTGATTTCTTCCCGGTTCCTCTGTCGGGCAAGCAGGGATTGGACGTGTCGGTGACGGGGAATTGGGTTAAACATGTGCTGAAGGTGAGTTTGGATGTTACTAGATATGAGTATTACACGGTGGGGAAGTATTATCATGAGCGAGATAGGTATGTTCCCGACAATACATCGGTGGACGGATGGAGCGGGCTGAGATATGACTATGGGAATTTCTATGCCTCAAAGACGTTTTATGATCCGGCGAAGAAGAGGAGGGTTTTGTGGGGTTGGGCTAATGAGTCTGATAGTGCTGTGGATGATATTTCTAAAGGATGGGCTGGAATTCAGGCAATTCCAAGGACGGTGTGGTTGGATCCTAATAGGAAGCAACTTTTGCAATGGCCTGTTGAGGAGCTGAATGGGCTGAGAGGGAAGCAAGTTAAGATGAGTCATCAGAAGCTGTACAAGGAACAACATGTTGAGGTTAAAGGAATTACTGCTGCCCAGGCTGATGTCGAAGTTACGTTCAGCTTTTCAAGCTTGGATAAAGCTGAGCCGTTTGATCCAAGCTGGGTGGATGCACAAGCAGTGTGTGGTAAACTGGGCTCAAAGGTCCAAGGTGGAGTTGGCCCGTTTGGGCTTCTGACACTGGCATCAGAGAACCTCCAGGAATTCACTCCTGTGTTCTTCAGGATTTTCAAGGCCCATCACAATCATGTGGTTCTTCTGTGCTCTGATGCAACAAGCTCGTCTCTGAAGAAGGATGGATTGTACAAGCCATCATTTGCAGGGTTCGTGGACATCGACTTATCGAGCAAAAAGCTCCCATTAAGAACTCTGATTGATCACTCGGTGGTGGAGAGTTTTGGAGGAGGTGGAAAAACGTGCATAACTTCAAGGGTATATCCAACAAAGGCGGTGTTTGGAGATGCACATTTGTATGTGTTCAACAATGGAAGCGAGGCTATCACAGTAGACTATCTAAGTGCTTGGAGTATGAGGAGTGCTACGATCAACTAA

Protein sequence

MNFFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSSLDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHNHVVLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN
Homology
BLAST of CmoCh06G004890 vs. ExPASy Swiss-Prot
Match: P26792 (Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota OX=4039 GN=INV1 PE=1 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 2.3e-243
Identity = 404/579 (69.78%), Postives = 474/579 (81.87%), Query Frame = 0

Query: 3   FFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPR 62
           F   +L++LL     +L IN     V A  + +  LQS+   +   +K +HRT +HFQP+
Sbjct: 17  FLQSLLAILL-VTTTTLHING----VEAFHEIHYNLQSVGAEN---VKQVHRTGYHFQPK 76

Query: 63  RHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFD 122
           ++WINDPNGPMY+ G+YHLFYQYNPKGA+WGNIVWAHSVS DL+NW  L+ AI+PSKPFD
Sbjct: 77  QNWINDPNGPMYYKGVYHLFYQYNPKGAVWGNIVWAHSVSTDLINWTPLEPAIFPSKPFD 136

Query: 123 INGCWSGSATVLPGNKPVILYTGV---DPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIV 182
             GC SGSAT+LPGNKPVILYTG+    P+N QVQNYAIPANLSDPYL  WIKPD+NP+V
Sbjct: 137 KYGCRSGSATILPGNKPVILYTGIVEGPPKNVQVQNYAIPANLSDPYLRKWIKPDNNPLV 196

Query: 183 DPDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSA 242
             ++G NA+AFRDPTTAWL  SGHWK ++GSKR +RG+AYLYRSKDF+KWTKAKHP+HS 
Sbjct: 197 VANNGENATAFRDPTTAWLDKSGHWKMLVGSKRNRRGIAYLYRSKDFIKWTKAKHPIHSQ 256

Query: 243 PNTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYV 302
            NTGMWECPDFFPV L G  GLD SVTG  VKHVLKVSLD+TRYEYYTVG Y  ++DRY+
Sbjct: 257 ANTGMWECPDFFPVSLKGLNGLDTSVTGESVKHVLKVSLDLTRYEYYTVGTYLTDKDRYI 316

Query: 303 PDNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIP 362
           PDNTSVDGW+GLRYDYGNFYASKTF+DP+K RR+LWGWANESDS   D++KGWAGIQ IP
Sbjct: 317 PDNTSVDGWAGLRYDYGNFYASKTFFDPSKNRRILWGWANESDSTAHDVAKGWAGIQLIP 376

Query: 363 RTVWLDPNRKQLLQWPVEELNGLRGKQVKMSH-QKLYKEQHVEVKGITAAQADVEVTFSF 422
           RT+WLDP+ KQL+QWP+EEL  LRG +VK S  Q L K   VEVKGITAAQADVEVTFSF
Sbjct: 377 RTLWLDPSGKQLMQWPIEELETLRGSKVKFSRKQDLSKGILVEVKGITAAQADVEVTFSF 436

Query: 423 SSLDKAEPFDPSWV--DAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAH 482
            SL K EPFDP W+  DA+ +C   GS VQGGVGPFGLLTLASE L+E+TPVFFR+FKA 
Sbjct: 437 KSLAKREPFDPKWLEYDAEKICSLKGSTVQGGVGPFGLLTLASEKLEEYTPVFFRVFKAQ 496

Query: 483 HNHVVLLCSDATSSSLKKDGLYKPSFAGFVDIDLSS-KKLPLRTLIDHSVVESFGGGGKT 542
           + H VL+CSDAT SSL K+GLY+PSFAGFVD+DL++ KK+ LR+LID+SVVESFG  GKT
Sbjct: 497 NTHKVLMCSDATRSSL-KEGLYRPSFAGFVDVDLATDKKISLRSLIDNSVVESFGAKGKT 556

Query: 543 CITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMR 575
           CI+SRVYPT AV+ +AHLYVFNNGSE ITV+ L AWSM+
Sbjct: 557 CISSRVYPTLAVYENAHLYVFNNGSETITVENLDAWSMK 586

BLAST of CmoCh06G004890 vs. ExPASy Swiss-Prot
Match: Q39693 (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 PE=3 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 6.3e-233
Identity = 382/579 (65.98%), Postives = 462/579 (79.79%), Query Frame = 0

Query: 4   FTKILSLLLGFAFFSLFIN-NGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPR 63
           F+   SL L   F  +F+N NGV   H   + +PELQS++  D   +K +HRT +HFQP+
Sbjct: 9   FSSDSSLFLLSIFSFIFLNINGVDSTH---RVFPELQSISAVD---VKLVHRTGYHFQPQ 68

Query: 64  RHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFD 123
           +HWINDPNGPM++ G YHLFYQYNPKG++WGNIVWAHSVS+DL+NW AL+ AI+PSKPFD
Sbjct: 69  KHWINDPNGPMFYKGYYHLFYQYNPKGSVWGNIVWAHSVSKDLINWIALEPAIFPSKPFD 128

Query: 124 INGCWSGSATVLPGNKPVILYTGV---DPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIV 183
             GCWSGSAT+LPGNKPVILYTG+   DP+N QVQNYA+PAN SDP+L +W+KPD+NP+V
Sbjct: 129 QYGCWSGSATILPGNKPVILYTGIVSPDPENAQVQNYAVPANYSDPFLREWVKPDNNPLV 188

Query: 184 DPDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSA 243
              +  N SAFRDPTTAW  + GHWK ++GS R+ RG+AYLYRSKDF KW ++ HP+H+ 
Sbjct: 189 GVHT-ENPSAFRDPTTAWF-DGGHWKMLVGSSRKHRGIAYLYRSKDFKKWKRSPHPIHTK 248

Query: 244 PNTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYV 303
             TGMWECPDF+PV    + GLD S  G  +KHVLKVSL+ TRYEYYT+G+Y   RD YV
Sbjct: 249 AETGMWECPDFYPVSPRSEDGLDNSKMGRGIKHVLKVSLNSTRYEYYTIGRYNRVRDFYV 308

Query: 304 PDNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIP 363
           PDNTSVDGW+GLRYDYGNFYASKTFYDP KKRR+LWGWANESDS +DD+ KGWAGIQ IP
Sbjct: 309 PDNTSVDGWAGLRYDYGNFYASKTFYDPIKKRRILWGWANESDSQIDDVQKGWAGIQLIP 368

Query: 364 RTVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFS 423
           R +WLDP+ +QL+QWP+EE+ GLRG ++ M +QKL    HVEV GITAAQADV+ TFSF 
Sbjct: 369 RRIWLDPSGRQLVQWPIEEVEGLRGSELHMRNQKLDMGVHVEVTGITAAQADVDATFSFK 428

Query: 424 SLDKAEPFDPSWV--DAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHH 483
           SLDKAE FDP W+  DAQ VC  +GS +QGG+GPFGLLTLAS++L+E+TPVFFRIFKA  
Sbjct: 429 SLDKAESFDPEWINLDAQDVCDSMGSTIQGGLGPFGLLTLASKDLEEYTPVFFRIFKAED 488

Query: 484 NHV-VLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTC 543
             + VL+CSDA  SSL  +GLYKPSF GFVD+DLS KK+ LR+LID+SVVESFG   K  
Sbjct: 489 QKLKVLMCSDAKRSSL-AEGLYKPSFRGFVDVDLSDKKISLRSLIDNSVVESFGAQRKNL 548

Query: 544 ITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRS 576
           I+SRVYPT A++ +AHL+VFNNG+E ITVD L AWSM S
Sbjct: 549 ISSRVYPTLAIYNNAHLFVFNNGTEPITVDNLDAWSMNS 578

BLAST of CmoCh06G004890 vs. ExPASy Swiss-Prot
Match: Q39692 (Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota OX=4039 GN=INV2 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 2.2e-230
Identity = 376/580 (64.83%), Postives = 460/580 (79.31%), Query Frame = 0

Query: 4   FTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPRR 63
           F  +L L      FSL +N  +  V A  + +PELQS++  +   ++ +HRT +HFQP++
Sbjct: 16  FHSMLFLSAVVFIFSLDVN--IRGVEASHQVFPELQSVSAVN---VQLVHRTGYHFQPKK 75

Query: 64  HWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDI 123
           HWINDPNGPMY+ G YHLFYQYNPKGA+WGNIVWAHS+S+DL+NW AL+ AI+PSKPFD 
Sbjct: 76  HWINDPNGPMYYKGFYHLFYQYNPKGAVWGNIVWAHSISKDLINWVALEPAIFPSKPFDK 135

Query: 124 NGCWSGSATVLPGNKPVILYTGV---DPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD 183
            GCWSGSATVLPG KPVI+YTG+    P N QVQN+A+PAN SDPYL +WIKPD+NPIV 
Sbjct: 136 YGCWSGSATVLPGGKPVIMYTGIVTPSPVNTQVQNFAVPANYSDPYLREWIKPDNNPIVR 195

Query: 184 PDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAP 243
             S  N+S+FRDPTTAW  +  HWK ++GS+R+ RG+AYLYRS++F+KWTKAKHPLHS  
Sbjct: 196 ARS-ENSSSFRDPTTAWF-DGVHWKILVGSRRKHRGIAYLYRSRNFLKWTKAKHPLHSKD 255

Query: 244 NTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP 303
            TGMWEC DF+PV   G  GLD SVTG  +KHVLKVSL  TRYEYYTVG+Y  + D YVP
Sbjct: 256 RTGMWECLDFYPVAPKGMNGLDTSVTGQDIKHVLKVSLYSTRYEYYTVGEYDRDNDIYVP 315

Query: 304 DNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPR 363
           DNTSVDGW+GLRYDYGNFYASKTF+DP K+RR+LWGWANESDS  DD+ KGWAGIQ IPR
Sbjct: 316 DNTSVDGWAGLRYDYGNFYASKTFFDPDKQRRILWGWANESDSKQDDVQKGWAGIQLIPR 375

Query: 364 TVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSS 423
            +WLDPN KQL+QWP+EE+  LRG+ V M  Q L   +H+EVKG+TAAQADV+ TFSF S
Sbjct: 376 KLWLDPNGKQLIQWPIEEIQLLRGQNVHMGSQVLNTGEHIEVKGVTAAQADVDATFSFKS 435

Query: 424 LDKAEPFDPSW--VDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHN 483
           LD+AE FDP+W  +DA  VC  +GS V+GG+GPFG LTLASE L+E+TPVFFR+FK    
Sbjct: 436 LDRAEWFDPNWAKLDALDVCDWMGSTVRGGLGPFGFLTLASEKLEEYTPVFFRVFKTKDK 495

Query: 484 HVVLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCIT 543
             VL+CSDA  SS   +GLYKP FAG+VD+DLS KK+ LR+LID+SVVESFG  G+TCIT
Sbjct: 496 LKVLMCSDAKRSSTTAEGLYKPPFAGYVDVDLSDKKISLRSLIDNSVVESFGAHGRTCIT 555

Query: 544 SRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATI 579
           SRVYP  A++ +AH++VFNNG+EAIT+D L AWSM++ ++
Sbjct: 556 SRVYPKIAIYNNAHVFVFNNGTEAITIDSLDAWSMKAPSL 588

BLAST of CmoCh06G004890 vs. ExPASy Swiss-Prot
Match: Q8W413 (Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana OX=3702 GN=CWINV4 PE=2 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 2.9e-214
Identity = 352/580 (60.69%), Postives = 437/580 (75.34%), Query Frame = 0

Query: 5   TKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPRRH 64
           + ++S+LL         N  +  + A  + Y ELQS +      +  LHR  FHFQP +H
Sbjct: 4   SNVISVLLLLLVLINLSNQNIKGIDAFHQIYEELQSESVES---VNHLHRPSFHFQPPKH 63

Query: 65  WINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDIN 124
           WINDPNGP+Y+ G+YHLFYQYN KGA+WGNI+WAHSVS+DLVNW+AL+ A+ PSK FDI 
Sbjct: 64  WINDPNGPVYYKGLYHLFYQYNTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIG 123

Query: 125 GCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPDSG 184
           G WSGS T++PG  P+ILYTGV+    Q+QNYAIP + SDPYL  WIKPDDNPI  PD  
Sbjct: 124 GTWSGSITIVPGKGPIILYTGVNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYT 183

Query: 185 VNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGM 244
           +N SAFRDPTTAW S  GHW+T++GSKR++RG+AY+YRS+DF  W KAKHP+HS  +TGM
Sbjct: 184 MNGSAFRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGM 243

Query: 245 WECPDFFPVPLSG-KQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNT 304
           WECPDFFPV L+  + GLD+   G   KHVLKVSLD+TRYEYYT+GKY  ++DRY+PD  
Sbjct: 244 WECPDFFPVSLTDFRNGLDLDYVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGN 303

Query: 305 SVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVW 364
           + DGW GLR+DYGNFYASKTF+D  K RR+LWGWANESD+  DDI KGWAG+Q IPRTV 
Sbjct: 304 TPDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVL 363

Query: 365 LDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSSLDK 424
           LD ++KQL+ WPVEE+  LRG  V+M++  +   Q +EVKGIT AQADVEVTF   SL+K
Sbjct: 364 LDSSKKQLVFWPVEEIESLRGNYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEK 423

Query: 425 AEPFDPSWV-DAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFK--AHHNHV 484
           AE FDPS+      +C   GS V+GGVGPFGL+TLA+ +L+E+TPVFFR+F     H   
Sbjct: 424 AEIFDPSFTWKPLELCNIKGSNVRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPK 483

Query: 485 VLLCSDATSSSLK-------KDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGG 544
           VL+CSDA  SSLK       KD +YKPSFAGFVD+D++  ++ LR+LIDHSVVESFG  G
Sbjct: 484 VLMCSDARPSSLKQDTGLLAKDRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALG 543

Query: 545 KTCITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSM 574
           KT ITSRVYP KAV  +AHLYVFNNG++ +T++ L+AW+M
Sbjct: 544 KTVITSRVYPVKAVKENAHLYVFNNGTQTVTIESLNAWNM 580

BLAST of CmoCh06G004890 vs. ExPASy Swiss-Prot
Match: Q1PEF8 (Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana OX=3702 GN=CWINV2 PE=2 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 2.5e-213
Identity = 354/565 (62.65%), Postives = 433/565 (76.64%), Query Frame = 0

Query: 22  NNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPRRHWINDPNGPMYFNGIYHL 81
           NNGV   H   K + +LQS + + ++V  PLHRT +HFQP RHWINDPN PM + G+YHL
Sbjct: 20  NNGVDAFH---KVFKKLQSKSTSLESV-SPLHRTAYHFQPPRHWINDPNAPMLYKGVYHL 79

Query: 82  FYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDINGCWSGSATVLPGNKPVI 141
           FYQYNPKGA+WGNIVWAHSVS+DL+NW+AL+ AIYPSK FDING WSGSAT +PG  PVI
Sbjct: 80  FYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVI 139

Query: 142 LYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPDSGVNASAFRDPTTAWLS-N 201
           LYTG+     Q+QNYAIP +LSDPYL  WIKPDDNPIV PD+G N SAFRDPTTAW +  
Sbjct: 140 LYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKK 199

Query: 202 SGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGMWECPDFFPVPLSGKQ- 261
            G+W+ ++GSKR+ RG+AY+Y+S+DF KW K+K P+HS   TGMWECPDFFPV ++ K+ 
Sbjct: 200 DGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKN 259

Query: 262 GLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNTSVDGWSGLRYDYGNFY 321
           GLD S  G   KHVLKVSLD+TRYEYYT+G Y  ++DRY PD  + DGW GLR+DYGN+Y
Sbjct: 260 GLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYY 319

Query: 322 ASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVWLDPNRKQLLQWPVEEL 381
           ASKTF+D    RR+LWGWANESD+  DD  KGWAGIQ IPRT+ LD + KQL+ WP+EE+
Sbjct: 320 ASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEI 379

Query: 382 NGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSSLDKAEPFDPSWVDAQ-AVC 441
             LRGK V+M++QK+   Q  EV+GIT AQ DV+VTF+  +L+KAE FD S+      +C
Sbjct: 380 ESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELC 439

Query: 442 GKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFK--AHHNHVVLLCSDATSSSLKKD- 501
              GS V GGVGPFGL+TLA+ +L+E+TPVFFR+FK  A +   VL+CSDA  SSLKKD 
Sbjct: 440 NLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDAKPSSLKKDT 499

Query: 502 ------GLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSRVYPTKAVFG 561
                  +YKPSFAGFVD+ L   K+ LR+LIDHSVVESFG  GKT ITSRVYPTKAV  
Sbjct: 500 GTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGE 559

Query: 562 DAHLYVFNNGSEAITVDYLSAWSMR 575
            AHL+VFNNGS+ +TV+ L+AW+M+
Sbjct: 560 KAHLFVFNNGSQPVTVESLNAWNMQ 580

BLAST of CmoCh06G004890 vs. ExPASy TrEMBL
Match: A0A6J1FK40 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444940 PE=3 SV=1)

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 579/579 (100.00%), Postives = 579/579 (100.00%), Query Frame = 0

Query: 1   MNFFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ 60
           MNFFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ
Sbjct: 1   MNFFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ 60

Query: 61  PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP 120
           PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP
Sbjct: 61  PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP 120

Query: 121 FDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD 180
           FDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD
Sbjct: 121 FDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD 180

Query: 181 PDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAP 240
           PDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAP
Sbjct: 181 PDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAP 240

Query: 241 NTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP 300
           NTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP
Sbjct: 241 NTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP 300

Query: 301 DNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPR 360
           DNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPR
Sbjct: 301 DNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPR 360

Query: 361 TVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSS 420
           TVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSS
Sbjct: 361 TVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSS 420

Query: 421 LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHNHV 480
           LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHNHV
Sbjct: 421 LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHNHV 480

Query: 481 VLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSR 540
           VLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSR
Sbjct: 481 VLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 580
           VYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN
Sbjct: 541 VYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 579

BLAST of CmoCh06G004890 vs. ExPASy TrEMBL
Match: A0A6J1L090 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111498679 PE=3 SV=1)

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 560/579 (96.72%), Postives = 572/579 (98.79%), Query Frame = 0

Query: 1   MNFFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ 60
           MNFF+KILSLLLGFA FS FINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ
Sbjct: 1   MNFFSKILSLLLGFALFSPFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ 60

Query: 61  PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP 120
           PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP
Sbjct: 61  PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP 120

Query: 121 FDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD 180
           FDINGCWSGSATVLPGNKPVILYTG+DPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD
Sbjct: 121 FDINGCWSGSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD 180

Query: 181 PDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAP 240
           PDSGVNASAFRDPTTAWLS+SGHWKTIIGSKRRKRGVAYLYRS+DFVKWTKAKHPLHSAP
Sbjct: 181 PDSGVNASAFRDPTTAWLSDSGHWKTIIGSKRRKRGVAYLYRSRDFVKWTKAKHPLHSAP 240

Query: 241 NTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP 300
           NTGMWECPDFFPVPLSG QGLDVSVTGN VKHVLKVSLDVTRYEYYTVGKYYHERDRYVP
Sbjct: 241 NTGMWECPDFFPVPLSGNQGLDVSVTGNLVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP 300

Query: 301 DNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPR 360
           DNTSVDGW+GLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDD+SKGWAGIQAIPR
Sbjct: 301 DNTSVDGWNGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDVSKGWAGIQAIPR 360

Query: 361 TVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSS 420
           TVWLDPNRKQLLQWPVEELN LRGKQVK+SHQKLYKEQHVEVKGITAAQADVEVTFSFSS
Sbjct: 361 TVWLDPNRKQLLQWPVEELNRLRGKQVKLSHQKLYKEQHVEVKGITAAQADVEVTFSFSS 420

Query: 421 LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHNHV 480
           LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENL+EFTPVFFRIFKAHHNHV
Sbjct: 421 LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLEEFTPVFFRIFKAHHNHV 480

Query: 481 VLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSR 540
           VLLCSDA+SSSLKK+GLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGK CITSR
Sbjct: 481 VLLCSDASSSSLKKEGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKICITSR 540

Query: 541 VYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 580
           VYPTKAVFGDAHLYVFNNG+EAITVDYLSAWSM SAT+N
Sbjct: 541 VYPTKAVFGDAHLYVFNNGTEAITVDYLSAWSMTSATVN 579

BLAST of CmoCh06G004890 vs. ExPASy TrEMBL
Match: A0A1S3B7H3 (beta-fructofuranosidase, insoluble isoenzyme 1 OS=Cucumis melo OX=3656 GN=LOC103486629 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.5e-303
Identity = 492/579 (84.97%), Postives = 533/579 (92.06%), Query Frame = 0

Query: 1   MNFFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ 60
           MN  T  LSLLLGF+FFSLFINNG   V AL K YPELQS+ + D +V+KPLHRTRFHFQ
Sbjct: 1   MNLSTTFLSLLLGFSFFSLFINNG---VEALHKIYPELQSI-QTDASVVKPLHRTRFHFQ 60

Query: 61  PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP 120
           PRR+WINDPNGPMYFNGIYHLFYQYNPKGA+WGNIVWAHS+SRDL+NWK LK AIYPSKP
Sbjct: 61  PRRNWINDPNGPMYFNGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKAAIYPSKP 120

Query: 121 FDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD 180
           FDINGCWSGSATVLPGNKPVILYTG+DPQNRQVQNYAIPANLSDPYLT+WIKPD NPIVD
Sbjct: 121 FDINGCWSGSATVLPGNKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDHNPIVD 180

Query: 181 PDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAP 240
           P  GVNASAFRDPTTAWLS SGHWKTIIGSKR++RG+AYLYRS+DFVKWTKAKHPLHSA 
Sbjct: 181 PGPGVNASAFRDPTTAWLSKSGHWKTIIGSKRKRRGMAYLYRSRDFVKWTKAKHPLHSAA 240

Query: 241 NTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP 300
           NTGMWECPDF+PVPL G+ GLD SVTGNWVKHVLKVSLD+TRYEYYTVGKY+ ++D+YVP
Sbjct: 241 NTGMWECPDFYPVPLRGRLGLDPSVTGNWVKHVLKVSLDLTRYEYYTVGKYFPKKDKYVP 300

Query: 301 DNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPR 360
           DNTSVDGWSGLRYDYGNFYASK+FYDP +KRRVLWGWANESDSA DD+SKGWAGIQ IPR
Sbjct: 301 DNTSVDGWSGLRYDYGNFYASKSFYDPLRKRRVLWGWANESDSAQDDVSKGWAGIQLIPR 360

Query: 361 TVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSS 420
           TVWLD N+KQL+QWPVEELN LRGK+V +SHQKL K   VEVKGITAAQADVEV FSFSS
Sbjct: 361 TVWLDHNQKQLIQWPVEELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVIFSFSS 420

Query: 421 LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHNHV 480
           LDKAEPFDP WVDAQA C +LG K QGGVGPFGLLTLASENL+EFTPVFFRIFKAHH HV
Sbjct: 421 LDKAEPFDPRWVDAQAACDQLGLKSQGGVGPFGLLTLASENLEEFTPVFFRIFKAHHKHV 480

Query: 481 VLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSR 540
           VL+CSDA SSSLK+DGLYKP+FAG+VDIDLSSKKL LR+LIDHSVVESFGGGGKTCITSR
Sbjct: 481 VLMCSDARSSSLKEDGLYKPAFAGYVDIDLSSKKLSLRSLIDHSVVESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 580
           VYPTK VF DAHL+VFNNG+EAITV+YLSAWSM+SA +N
Sbjct: 541 VYPTKGVFNDAHLHVFNNGTEAITVEYLSAWSMKSARVN 575

BLAST of CmoCh06G004890 vs. ExPASy TrEMBL
Match: A0A0A0LAY1 (Cell wall invertase 3 OS=Cucumis sativus OX=3659 GN=Csa_3G777580 PE=2 SV=1)

HSP 1 Score: 1039.3 bits (2686), Expect = 6.2e-300
Identity = 489/579 (84.46%), Postives = 532/579 (91.88%), Query Frame = 0

Query: 1   MNFFTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQ 60
           MN FT  LSLLLGF+FFSLFINNGVVFV AL K YPELQS+ + D +VIKPLHRTRFHFQ
Sbjct: 1   MNLFT-FLSLLLGFSFFSLFINNGVVFVDALHKIYPELQSI-QTDASVIKPLHRTRFHFQ 60

Query: 61  PRRHWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKP 120
           PRR+WINDPNGPMYF GIYHLFYQYNPKGA+WGNIVWAHS+SRDL+NWK LK AIYPSKP
Sbjct: 61  PRRNWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSISRDLINWKPLKPAIYPSKP 120

Query: 121 FDINGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVD 180
           FDINGCWSGSATVLPG+KPVILYTG+DPQNRQVQNYAIPANLSDPYLT+WIKPD+NPIVD
Sbjct: 121 FDINGCWSGSATVLPGDKPVILYTGIDPQNRQVQNYAIPANLSDPYLTEWIKPDNNPIVD 180

Query: 181 PDSGVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAP 240
           P  GVNASAFRDPTTAWLS SGHWKT+IGSKR+KRG+AYLYRS+DFVKWTKAKHPLHSA 
Sbjct: 181 PGPGVNASAFRDPTTAWLSKSGHWKTVIGSKRKKRGMAYLYRSRDFVKWTKAKHPLHSAA 240

Query: 241 NTGMWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVP 300
           NTGMWECPDF+PVPL GK GLD SVTGNWVKHV KVSLD+TRYEYYTVGKY+ ++D+YVP
Sbjct: 241 NTGMWECPDFYPVPLRGKLGLDPSVTGNWVKHVFKVSLDLTRYEYYTVGKYFPKKDKYVP 300

Query: 301 DNTSVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPR 360
           DNTSVDGWSGLRYDYGNFYASK+FYDP KKRRVLWGWANESDSA DD+SKGWAGIQ IPR
Sbjct: 301 DNTSVDGWSGLRYDYGNFYASKSFYDPLKKRRVLWGWANESDSAQDDVSKGWAGIQLIPR 360

Query: 361 TVWLDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSS 420
           TVWLD N++QL QWPV+ELN LRGK+V +SHQKL K   VEVKGITAAQADVEV FSFSS
Sbjct: 361 TVWLDHNQRQLKQWPVKELNTLRGKKVVLSHQKLLKGHTVEVKGITAAQADVEVMFSFSS 420

Query: 421 LDKAEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHNHV 480
           LDKA+PFDP WVDAQA C +LG+  QGGVGPFGLLTLASENL EFTPVFFRIFKAHH HV
Sbjct: 421 LDKADPFDPRWVDAQAACDQLGNS-QGGVGPFGLLTLASENLDEFTPVFFRIFKAHHKHV 480

Query: 481 VLLCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSR 540
           VL+CSDA SSSLK++GLYKP+FAG+VDIDLS KKL LR+LIDHSVVESFGGGGKTCITSR
Sbjct: 481 VLMCSDARSSSLKEEGLYKPAFAGYVDIDLSRKKLSLRSLIDHSVVESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 580
           VYPTK VF DAHL+VFNNG+EAITV+YL AWSM+SA +N
Sbjct: 541 VYPTKGVFDDAHLHVFNNGTEAITVEYLRAWSMKSARMN 576

BLAST of CmoCh06G004890 vs. ExPASy TrEMBL
Match: A0A6J1CU71 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Momordica charantia OX=3673 GN=LOC111014380 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.6e-287
Identity = 463/577 (80.24%), Postives = 515/577 (89.25%), Query Frame = 0

Query: 4   FTKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPRR 63
           F + LSLLL F+  S  +NNGV    +  + YPELQSL R D + I+PLHRTRFHFQPRR
Sbjct: 3   FLRFLSLLLVFSSLSFLLNNGV--AQSRHEIYPELQSL-RVDTSFIRPLHRTRFHFQPRR 62

Query: 64  HWINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDI 123
           HWINDPNGPMYF GIYHLFYQYNPKGA+WGNIVWAHS SRDL+NWKAL HA+ PSKPFDI
Sbjct: 63  HWINDPNGPMYFKGIYHLFYQYNPKGAVWGNIVWAHSFSRDLINWKALPHALSPSKPFDI 122

Query: 124 NGCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPDS 183
           NGCWSGSAT+LPG +PVI+YTG+DP NRQVQNYA+PANLSDPYL DW+KPD NP+VDPDS
Sbjct: 123 NGCWSGSATILPGGRPVIMYTGIDPHNRQVQNYAVPANLSDPYLRDWVKPDFNPVVDPDS 182

Query: 184 GVNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTG 243
           GVNASAFRDPTT W   SGHW+T+IGS+R  RG+AYLYRS+DFVKWT+AKHPLHS  NTG
Sbjct: 183 GVNASAFRDPTTGWRGKSGHWRTVIGSRRGSRGMAYLYRSRDFVKWTRAKHPLHSVANTG 242

Query: 244 MWECPDFFPVPLSGKQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNT 303
           MWECPDF+PVPL+GK+GLD SV+G  VKHVLKVSLD+TRY+YYTVGKY   +DRYVPDNT
Sbjct: 243 MWECPDFYPVPLTGKRGLDTSVSGGGVKHVLKVSLDLTRYDYYTVGKYLENKDRYVPDNT 302

Query: 304 SVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVW 363
           S DGW GLRYDYGN+YASKTFYD  KKRRVLWGWANESD+A DD++KGWAGIQAIPRTVW
Sbjct: 303 SPDGWLGLRYDYGNYYASKTFYDAGKKRRVLWGWANESDTARDDVAKGWAGIQAIPRTVW 362

Query: 364 LDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSSLDK 423
           LDPNRKQLLQWPVEE+N LRG +V+M+++KL K QHVE+ GI+AAQADVEVTFSFSSLDK
Sbjct: 363 LDPNRKQLLQWPVEEVNRLRGMKVEMTNRKLAKGQHVEIHGISAAQADVEVTFSFSSLDK 422

Query: 424 AEPFDPSWVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHN-HVVL 483
           AEPFDP WVDAQ+VCG+LGS  QGGVGPFGLLTLAS++L EFTPVFFRIFKAHHN HVVL
Sbjct: 423 AEPFDPRWVDAQSVCGRLGSTAQGGVGPFGLLTLASKHLDEFTPVFFRIFKAHHNKHVVL 482

Query: 484 LCSDATSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSRVY 543
           LCSDATSSSL+  GLYKPSFAGFVDIDLSSKKL LRTLIDHS+VESFGGGGK CITSRVY
Sbjct: 483 LCSDATSSSLRTKGLYKPSFAGFVDIDLSSKKLALRTLIDHSMVESFGGGGKACITSRVY 542

Query: 544 PTKAVFGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 580
           PTKAVFG+AHL+VFNNGSE ITVD LSAWSM+SA +N
Sbjct: 543 PTKAVFGNAHLHVFNNGSELITVDNLSAWSMKSARVN 576

BLAST of CmoCh06G004890 vs. TAIR 10
Match: AT2G36190.1 (cell wall invertase 4 )

HSP 1 Score: 746.1 bits (1925), Expect = 2.1e-215
Identity = 352/580 (60.69%), Postives = 437/580 (75.34%), Query Frame = 0

Query: 5   TKILSLLLGFAFFSLFINNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPRRH 64
           + ++S+LL         N  +  + A  + Y ELQS +      +  LHR  FHFQP +H
Sbjct: 4   SNVISVLLLLLVLINLSNQNIKGIDAFHQIYEELQSESVES---VNHLHRPSFHFQPPKH 63

Query: 65  WINDPNGPMYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDIN 124
           WINDPNGP+Y+ G+YHLFYQYN KGA+WGNI+WAHSVS+DLVNW+AL+ A+ PSK FDI 
Sbjct: 64  WINDPNGPVYYKGLYHLFYQYNTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIG 123

Query: 125 GCWSGSATVLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPDSG 184
           G WSGS T++PG  P+ILYTGV+    Q+QNYAIP + SDPYL  WIKPDDNPI  PD  
Sbjct: 124 GTWSGSITIVPGKGPIILYTGVNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYT 183

Query: 185 VNASAFRDPTTAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGM 244
           +N SAFRDPTTAW S  GHW+T++GSKR++RG+AY+YRS+DF  W KAKHP+HS  +TGM
Sbjct: 184 MNGSAFRDPTTAWFSKDGHWRTVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGM 243

Query: 245 WECPDFFPVPLSG-KQGLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNT 304
           WECPDFFPV L+  + GLD+   G   KHVLKVSLD+TRYEYYT+GKY  ++DRY+PD  
Sbjct: 244 WECPDFFPVSLTDFRNGLDLDYVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGN 303

Query: 305 SVDGWSGLRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVW 364
           + DGW GLR+DYGNFYASKTF+D  K RR+LWGWANESD+  DDI KGWAG+Q IPRTV 
Sbjct: 304 TPDGWEGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVL 363

Query: 365 LDPNRKQLLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSSLDK 424
           LD ++KQL+ WPVEE+  LRG  V+M++  +   Q +EVKGIT AQADVEVTF   SL+K
Sbjct: 364 LDSSKKQLVFWPVEEIESLRGNYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEK 423

Query: 425 AEPFDPSWV-DAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFK--AHHNHV 484
           AE FDPS+      +C   GS V+GGVGPFGL+TLA+ +L+E+TPVFFR+F     H   
Sbjct: 424 AEIFDPSFTWKPLELCNIKGSNVRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPK 483

Query: 485 VLLCSDATSSSLK-------KDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGG 544
           VL+CSDA  SSLK       KD +YKPSFAGFVD+D++  ++ LR+LIDHSVVESFG  G
Sbjct: 484 VLMCSDARPSSLKQDTGLLAKDRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALG 543

Query: 545 KTCITSRVYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSM 574
           KT ITSRVYP KAV  +AHLYVFNNG++ +T++ L+AW+M
Sbjct: 544 KTVITSRVYPVKAVKENAHLYVFNNGTQTVTIESLNAWNM 580

BLAST of CmoCh06G004890 vs. TAIR 10
Match: AT3G52600.1 (cell wall invertase 2 )

HSP 1 Score: 743.0 bits (1917), Expect = 1.8e-214
Identity = 354/565 (62.65%), Postives = 433/565 (76.64%), Query Frame = 0

Query: 22  NNGVVFVHALPKFYPELQSLNRADDAVIKPLHRTRFHFQPRRHWINDPNGPMYFNGIYHL 81
           NNGV   H   K + +LQS + + ++V  PLHRT +HFQP RHWINDPN PM + G+YHL
Sbjct: 20  NNGVDAFH---KVFKKLQSKSTSLESV-SPLHRTAYHFQPPRHWINDPNAPMLYKGVYHL 79

Query: 82  FYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDINGCWSGSATVLPGNKPVI 141
           FYQYNPKGA+WGNIVWAHSVS+DL+NW+AL+ AIYPSK FDING WSGSAT +PG  PVI
Sbjct: 80  FYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVI 139

Query: 142 LYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPDSGVNASAFRDPTTAWLS-N 201
           LYTG+     Q+QNYAIP +LSDPYL  WIKPDDNPIV PD+G N SAFRDPTTAW +  
Sbjct: 140 LYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKK 199

Query: 202 SGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGMWECPDFFPVPLSGKQ- 261
            G+W+ ++GSKR+ RG+AY+Y+S+DF KW K+K P+HS   TGMWECPDFFPV ++ K+ 
Sbjct: 200 DGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKN 259

Query: 262 GLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNTSVDGWSGLRYDYGNFY 321
           GLD S  G   KHVLKVSLD+TRYEYYT+G Y  ++DRY PD  + DGW GLR+DYGN+Y
Sbjct: 260 GLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYY 319

Query: 322 ASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVWLDPNRKQLLQWPVEEL 381
           ASKTF+D    RR+LWGWANESD+  DD  KGWAGIQ IPRT+ LD + KQL+ WP+EE+
Sbjct: 320 ASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEI 379

Query: 382 NGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSSLDKAEPFDPSWVDAQ-AVC 441
             LRGK V+M++QK+   Q  EV+GIT AQ DV+VTF+  +L+KAE FD S+      +C
Sbjct: 380 ESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELC 439

Query: 442 GKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFK--AHHNHVVLLCSDATSSSLKKD- 501
              GS V GGVGPFGL+TLA+ +L+E+TPVFFR+FK  A +   VL+CSDA  SSLKKD 
Sbjct: 440 NLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDAKPSSLKKDT 499

Query: 502 ------GLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSRVYPTKAVFG 561
                  +YKPSFAGFVD+ L   K+ LR+LIDHSVVESFG  GKT ITSRVYPTKAV  
Sbjct: 500 GTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGE 559

Query: 562 DAHLYVFNNGSEAITVDYLSAWSMR 575
            AHL+VFNNGS+ +TV+ L+AW+M+
Sbjct: 560 KAHLFVFNNGSQPVTVESLNAWNMQ 580

BLAST of CmoCh06G004890 vs. TAIR 10
Match: AT3G52600.2 (cell wall invertase 2 )

HSP 1 Score: 689.5 bits (1778), Expect = 2.3e-198
Identity = 326/514 (63.42%), Postives = 398/514 (77.43%), Query Frame = 0

Query: 73  MYFNGIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDINGCWSGSAT 132
           M + G+YHLFYQYNPKGA+WGNIVWAHSVS+DL+NW+AL+ AIYPSK FDING WSGSAT
Sbjct: 1   MLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSAT 60

Query: 133 VLPGNKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPDSGVNASAFRD 192
            +PG  PVILYTG+     Q+QNYAIP +LSDPYL  WIKPDDNPIV PD+G N SAFRD
Sbjct: 61  HVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRD 120

Query: 193 PTTAWLS-NSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGMWECPDFF 252
           PTTAW +   G+W+ ++GSKR+ RG+AY+Y+S+DF KW K+K P+HS   TGMWECPDFF
Sbjct: 121 PTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFF 180

Query: 253 PVPLSGKQ-GLDVSVTGNWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNTSVDGWSG 312
           PV ++ K+ GLD S  G   KHVLKVSLD+TRYEYYT+G Y  ++DRY PD  + DGW G
Sbjct: 181 PVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDG 240

Query: 313 LRYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVWLDPNRKQ 372
           LR+DYGN+YASKTF+D    RR+LWGWANESD+  DD  KGWAGIQ IPRT+ LD + KQ
Sbjct: 241 LRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQ 300

Query: 373 LLQWPVEELNGLRGKQVKMSHQKLYKEQHVEVKGITAAQADVEVTFSFSSLDKAEPFDPS 432
           L+ WP+EE+  LRGK V+M++QK+   Q  EV+GIT AQ DV+VTF+  +L+KAE FD S
Sbjct: 301 LVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDES 360

Query: 433 WVDAQ-AVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFK--AHHNHVVLLCSDA 492
           +      +C   GS V GGVGPFGL+TLA+ +L+E+TPVFFR+FK  A +   VL+CSDA
Sbjct: 361 FATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDA 420

Query: 493 TSSSLKKD-------GLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSR 552
             SSLKKD        +YKPSFAGFVD+ L   K+ LR+LIDHSVVESFG  GKT ITSR
Sbjct: 421 KPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSR 480

Query: 553 VYPTKAVFGDAHLYVFNNGSEAITVDYLSAWSMR 575
           VYPTKAV   AHL+VFNNGS+ +TV+ L+AW+M+
Sbjct: 481 VYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 514

BLAST of CmoCh06G004890 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 668.3 bits (1723), Expect = 5.5e-192
Identity = 325/572 (56.82%), Postives = 407/572 (71.15%), Query Frame = 0

Query: 18  SLFINNGVVFVHALPKFYPEL-QSLNRADDAVIKPLHRTRFHFQPRRHWINDPNGPMYFN 77
           +L I N VV + A    Y  L QS N    +V +P +RT FHFQP ++W+NDPNGPM + 
Sbjct: 16  TLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQP-YRTGFHFQPPKNWMNDPNGPMIYK 75

Query: 78  GIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDINGCWSGSATVLPG 137
           GIYHLFYQ+NPKGA+WGNIVWAHS S DL+NW     AI+PS PFDINGCWSGSAT+LP 
Sbjct: 76  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 135

Query: 138 NKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPD--SGVNASAFRDPT 197
            KPVILYTG+DP+N+QVQN A P NLSDPYL +W K   NP++ PD  +G+NAS+FRDPT
Sbjct: 136 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 195

Query: 198 TAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGMWECPDFFPVP 257
           TAWL     W+ IIGSK  +RG+A  Y SKDF+KW K+  PLH    +GMWECPDFFPV 
Sbjct: 196 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 255

Query: 258 LSGKQGLDVSVTG---NWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNTSVDGWSGL 317
             G  G++ S  G     +KHVLK+SLD T+++YYT+G Y   +D++VPDN      +  
Sbjct: 256 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 315

Query: 318 RYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVWLDPNRKQL 377
           RYDYG +YASKTF+D AK RR+LWGW NES S  DD+ KGW+GIQ IPR +WLD + KQL
Sbjct: 316 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 375

Query: 378 LQWPVEELNGLRGKQVKMSHQKLYKE-QHVEVKGITAAQADVEVTFSFSSLDKAEPFDPS 437
           +QWPV E+  LR KQVK    K+ K    +EV G+TAAQADVEV F    L+KA+  +PS
Sbjct: 376 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 435

Query: 438 WVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHN---HVVLLCSDA 497
           W D Q +C K+   V+ G+GPFGL+ LAS+NL+E+T V+FRIFKA  N   +VVL+CSD 
Sbjct: 436 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 495

Query: 498 TSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSRVYPTKAV 557
           + SSLK+D   K ++  FVDI+   + L LR LIDHSVVESFGG G+ CITSRVYP  A+
Sbjct: 496 SRSSLKEDN-DKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAI 555

Query: 558 FGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 580
              +HL+ FN G +++ V  L+AWSM SA I+
Sbjct: 556 GKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of CmoCh06G004890 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 655.6 bits (1690), Expect = 3.7e-188
Identity = 322/572 (56.29%), Postives = 404/572 (70.63%), Query Frame = 0

Query: 18  SLFINNGVVFVHALPKFYPEL-QSLNRADDAVIKPLHRTRFHFQPRRHWINDPNGPMYFN 77
           +L I N VV + A    Y  L QS N    +V +P +RT FHFQP ++W+   NGPM + 
Sbjct: 16  TLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQP-YRTGFHFQPPKNWM---NGPMIYK 75

Query: 78  GIYHLFYQYNPKGAIWGNIVWAHSVSRDLVNWKALKHAIYPSKPFDINGCWSGSATVLPG 137
           GIYHLFYQ+NPKGA+WGNIVWAHS S DL+NW     AI+PS PFDINGCWSGSAT+LP 
Sbjct: 76  GIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILPN 135

Query: 138 NKPVILYTGVDPQNRQVQNYAIPANLSDPYLTDWIKPDDNPIVDPD--SGVNASAFRDPT 197
            KPVILYTG+DP+N+QVQN A P NLSDPYL +W K   NP++ PD  +G+NAS+FRDPT
Sbjct: 136 GKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDPT 195

Query: 198 TAWLSNSGHWKTIIGSKRRKRGVAYLYRSKDFVKWTKAKHPLHSAPNTGMWECPDFFPVP 257
           TAWL     W+ IIGSK  +RG+A  Y SKDF+KW K+  PLH    +GMWECPDFFPV 
Sbjct: 196 TAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPVT 255

Query: 258 LSGKQGLDVSVTG---NWVKHVLKVSLDVTRYEYYTVGKYYHERDRYVPDNTSVDGWSGL 317
             G  G++ S  G     +KHVLK+SLD T+++YYT+G Y   +D++VPDN      +  
Sbjct: 256 RFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTAP 315

Query: 318 RYDYGNFYASKTFYDPAKKRRVLWGWANESDSAVDDISKGWAGIQAIPRTVWLDPNRKQL 377
           RYDYG +YASKTF+D AK RR+LWGW NES S  DD+ KGW+GIQ IPR +WLD + KQL
Sbjct: 316 RYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQL 375

Query: 378 LQWPVEELNGLRGKQVKMSHQKLYKE-QHVEVKGITAAQADVEVTFSFSSLDKAEPFDPS 437
           +QWPV E+  LR KQVK    K+ K    +EV G+TAAQADVEV F    L+KA+  +PS
Sbjct: 376 IQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEPS 435

Query: 438 WVDAQAVCGKLGSKVQGGVGPFGLLTLASENLQEFTPVFFRIFKAHHN---HVVLLCSDA 497
           W D Q +C K+   V+ G+GPFGL+ LAS+NL+E+T V+FRIFKA  N   +VVL+CSD 
Sbjct: 436 WTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQ 495

Query: 498 TSSSLKKDGLYKPSFAGFVDIDLSSKKLPLRTLIDHSVVESFGGGGKTCITSRVYPTKAV 557
           + SSLK+D   K ++  FVDI+   + L LR LIDHSVVESFGG G+ CITSRVYP  A+
Sbjct: 496 SRSSLKEDN-DKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAI 555

Query: 558 FGDAHLYVFNNGSEAITVDYLSAWSMRSATIN 580
              +HL+ FN G +++ V  L+AWSM SA I+
Sbjct: 556 GKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P267922.3e-24369.78Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota OX=4039 GN=INV1 ... [more]
Q396936.3e-23365.98Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 ... [more]
Q396922.2e-23064.83Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota OX=4039 GN=INV2 ... [more]
Q8W4132.9e-21460.69Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana OX=3... [more]
Q1PEF82.5e-21362.65Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1FK400.0e+00100.00beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=366... [more]
A0A6J1L0900.0e+0096.72beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 ... [more]
A0A1S3B7H33.5e-30384.97beta-fructofuranosidase, insoluble isoenzyme 1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0LAY16.2e-30084.46Cell wall invertase 3 OS=Cucumis sativus OX=3659 GN=Csa_3G777580 PE=2 SV=1[more]
A0A6J1CU714.6e-28780.24beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
AT2G36190.12.1e-21560.69cell wall invertase 4 [more]
AT3G52600.11.8e-21462.65cell wall invertase 2 [more]
AT3G52600.22.3e-19863.42cell wall invertase 2 [more]
AT3G13790.15.5e-19256.82Glycosyl hydrolases family 32 protein [more]
AT3G13790.23.7e-18856.29Glycosyl hydrolases family 32 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 58..533
e-value: 1.2E-247
score: 838.6
NoneNo IPR availableGENE3D2.60.120.560coord: 389..573
e-value: 3.0E-77
score: 260.6
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 5..579
NoneNo IPR availablePANTHERPTHR31953:SF65BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME 1-LIKEcoord: 5..579
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 64..364
e-value: 9.28033E-170
score: 481.886
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 58..375
e-value: 7.3E-106
score: 354.2
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 378..571
e-value: 2.5E-26
score: 92.5
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 62..388
e-value: 1.3E-121
score: 408.1
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 49..376
IPR018053Glycoside hydrolase, family 32, active sitePROSITEPS00609GLYCOSYL_HYDROL_F32coord: 58..71
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 375..576

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G004890.1CmoCh06G004890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds