CmoCh06G004660 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G004660
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase At5g03610-like
LocationCmo_Chr06: 2258074 .. 2262153 (-)
RNA-Seq ExpressionCmoCh06G004660
SyntenyCmoCh06G004660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAGAAGCCTTAGACGCCACAACTTCGAAGCGCGATGTTTTAAACGTCGCGTAAAAAACTAGCAGAAAATGGAGCGGTTGACGTAAGAATGGTGAAGGGCAGGTGATTTTATCATTGGGAGATTACTATATAAGCTATTAAAGAGAGTGAGTACAGTTTCATTTGGAACATTTGGGTAAAGAGACAACACAACTTCAAAGAGATTCCAATTCCAATTCCAATTCTAAGCTCCTTTAATGGACTGCAAAAAGCTCCTCTGTTTCTCTCTCCTCTGCTTCTCTCTCCTCCCAGGTTCTCCATTTTCTCTCTCTAAAACCCCCTCTTCCCCTTCCTTTCTCTCTCTAAAATATATATTTTTTTAAAATTTGTTTTTGGGTTGCAGAATTATATGGGGTGGTTTGGGTTTCTGTTGGAGGGGGTGGCCACCGTCATCGCCACCGCGTGCGGCGGAGTGGACAGAGTCTTGGTGGAGGGGAGCTGTCGGATTCGAATCCTAGAAAGCTGTTTGTGTTTGGGGATTCTTATGTAGACACCGGAAATAACCGGAAACCGGCGGCAAAATCTTGGCAGTATCCTTATGGAATTACTTTTCCCGGTAAACCCACCGGCCGTTTCTCCGATGGCCGTGTTTTGACCGATTACCTAGGTAACTCTGAAAATCTAACCGTCTCTTTCTCTGTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCGTGTATTTTGAATTATTAATTGTTTTGAATTTATTTAAGATTCGTTTAATTTGGATAATTATGAAATTATGTTATTTTTAAAATTATATTGTTTTTAAAAAATCATTTATTGAGCTAGATTAATAATTTATTTAGTTTTTTATTAACTAATTTTAAAATTTTAGCAAAAGAAAGAAAAAATCACAATGAAAAAAGGACTAAAGTAGTGAAGAAGGCTCTTTATATTTAAAAACAAAAAGAATTAAAATTGTATGATTAAAGGAAATTCTAAACACAAAATAAAAATAATATTTAAAATTATTTAAAAAGCAAATAATAAAGATAAATTTTCTTTAGTTTTATGGATATAAATAGAGGTGGTGGCCCACTCCACATTGCTATTGTATTCTTTTTTCTTTTTTCTTTTTTCCTTTCAAAATAATTTATTATTAAACACTTTAAATAGCACATTTTTTCCTTTAATAAAAATTAAAAAAAAAACCTCGTGGTAATAATTTTTTTAAAATTTTATGTATTTCAAAAATTATAAAATTATACCATATCTTATCTATTTATTATTGAAACATTTAAACTCTTATCTAAATTCTATTTTTTTTTCAAAAATTATTAGTGATTTATAAATTTAATAAAGTAAATAATAAAAAAATAAAACAATTTCACAAAATAAATAATATATTTTAAAAATTAAATGGAAAATAATGAAATAAATTTAAAAAATCAGTGCAAAAGGAAGTTTATTTTTTATTTTTTATTTTTATTTTTTAATTTCAGTCAGAGCGTGGCACTTTTTGGCTGTAAAAACTCAAGACCGGCTGCATTATAGAGTCGGCTTTTTTATTTTATTTTATTTTTATTTTTATTTTTGCTTTATTGATATGGAGATGGCTGTGACTTTTGAGCATGTCAGTTTAGCGTAACCCTTTTCCTTTTCCCTGACAGTCAGTCTTTATCTTCAACTTCACTGCATCTTTTCCTGTCATTTTCCCCTTTTTTTTTTCTCGATTTTATTTATTTTTTAATTATAGAATATATATTTTAATTTGACCAATTAAGGTATATTCTAGAATTTTTTATTTCTTGTGTTATTAAAATGTCGGTGGAATTAAAATTGGGTTTAATTAAGAACTGAGTTTAAATATATTGGAATTTCACATTATTTTTTTTAATTAGTTTATGGTTAATATAATTTTTTTTATTTTTTTTATTTTTTAATGAATCATAAAAATTGGCTCCATTTCGCGGCGCCATTGGGTGGTTGAATCTTGTGTGTGCAGCCAAGTATCTGAGAGTGAAGTCTCCGATTGCGTACAAATGGCGGAAAGTAGGGTTAGGGCAACTGAAATACGGGATGAATTTCGCGTACGGCGGTACCGGCGTTTTCAATACCTTTATAATGAGCCCTAACATGAGCACTCAGATCGACTTCCTCCAGCAGCTGATCCACGAATCGACGTATACCGCCCAAGATTTGCATTACTCTGTGGCGCTGATCTCCCTCGCCGGCAACGATTATGGCGTCTACCAGGCCACTGACGGCTCTGCTCAGGTTTTCCCCAACATTTGTAACGTTTAATTTCAACTCTATGAATTGATTTTTTAGTCAAAATATAAGAAGTTAAGCAAAGAATTTGGGGATTTTGTATTGAGTTTGGCGTGTTGGTGGCAGGGGTGGCAGCCATTTATAACGAAAGTGGTGAACCAGCTGGAGGTGAATTTGAGGCGCATCCATGGGCTGGGGGTTCCAAAGATAGTGGTGACGTCTCTGGAGCCGCTGGGCTGTCTTCCTAGCAGCACAGTGGCATCCTCATTCCATCAGTGTAATGCCACTGAGAATCTGCTTGTCAACTTCCACAATCTGCTATTGCAGCAATCTGTGGCAAAGCTCAACAGTGAAGTAAAAGATGCCTCTGCATCCACTTTCATCCTTCTTGATCTGTATTCCTCTTTCATGGCTGCTCTCAACAACAAAGGAGACCCTCTTGGTGACTCTCTTGATCTTTTGATCTTTTGATTTTTTTTAGTCCACTAATTCTCTTATGCTCCTTAAAAATTGTTTAGTCCCATTGATTATTCGAAACCCACATGAGAGCTGACATTCTTCAACTAAAACACCCCTGATTTACCATCTTACCAGGATTTGTCCTCATGAGTCGGCCTAATGGTCAAATATCAATGGGTTAGAGAAAAAGGACATCCCAATAAGTCTTGTGTCCTCCTGCAAAGGATTCAGGCTTTGGGCGCCACATGCTTATGATTTAATATCCTATTTGACAGCAACTGTATAGTAGGATTAGACATGTGTTCTTTAAGAATAGTAAGGTGCATGTCAGCTGGTTTAGATACTCATATATATTTAAATAAAAAGACTGATTTACATGAATGAACAACAAGATCATGACATCGTACGTGTTTGCTATTGGACAAATGTGTAACAACCCAAGCCCACCACTATATGATTTTGTCCTCTTTGTGCTTTCCCTTTTGGGTTTCCCTTCACAATTTTTAAAACATGTCTGATAGGGAGAGGTTTCCACACTCTTATAAAGAATATTTCGTTCCCCTCTTCAATCGATGTGGGATCTCATAGAATGCGACTTCTTTTAGTTAATTTGGCTCTCCATTTATGAGAAGATTGTGTTCGAGATCTGCTAAACTAAGTCTTGGTTCTTCTGGAAATAGGTTGATGTTTAGCATATTAGAGACTACAGCTGGGTTCCCTTGACAAGCTAGACAAAGTGACTCCTGCTCCTTAATTATTCTGGCTTTTGGTTGTAATCCCTACAAAAGGTTTCCGCTCTGTAAACTATGCTTGGTACCTCTTAGGTCTCGTCTCAAAATATGGTATAGATTAAGATTCTTGCCTTCCATCACGAGCTAGAAAAGGGTCAATTGACTGACTTGACACTCCTTCATAAGAAGCCCACAAGCACACCTCCCTTTAAAAGTTTATAGGGTGTTTTTTAAGTGCTTACCATGTTTTGTGCAGGGAACGTGAAATTTGAGAACCCATTGAAGCCATGTTGTGTTGGAATAAGCAGCGAGTATGCATGTGGGAATGTGGGTGCGAATGGGGAAAAGAAGTACACCATATGTGAAGATCCAGGAGCTGCATTCTTCTGGGACGAAGTGCACCCTTCTCAAAATGGCTGGTTCGCTGTGTACTCAGCATTGCAAGCAAATTTAAAGCAACTCTAAGCTTTAAATTACATAGAAATCAGATAAGTGACCCCCCAATTTGACAAGACATTTCATTCATTCAATGATCATCTGTCTTGTAGTATTCAAATAATATTTGGCATAGCCCCTTTGCCTCTGCTCTCTGATTTGCTTGTAGGTATTCATCTTTCTCGCCCCNNNNNN

mRNA sequence

CCAAAGAAGCCTTAGACGCCACAACTTCGAAGCGCGATGTTTTAAACGTCGCGTAAAAAACTAGCAGAAAATGGAGCGGTTGACGTAAGAATGGTGAAGGGCAGGTGATTTTATCATTGGGAGATTACTATATAAGCTATTAAAGAGAGTGAGTACAGTTTCATTTGGAACATTTGGGTAAAGAGACAACACAACTTCAAAGAGATTCCAATTCCAATTCCAATTCTAAGCTCCTTTAATGGACTGCAAAAAGCTCCTCTGTTTCTCTCTCCTCTGCTTCTCTCTCCTCCCAGAATTATATGGGGTGGTTTGGGTTTCTGTTGGAGGGGGTGGCCACCGTCATCGCCACCGCGTGCGGCGGAGTGGACAGAGTCTTGGTGGAGGGGAGCTGTCGGATTCGAATCCTAGAAAGCTGTTTGTGTTTGGGGATTCTTATGTAGACACCGGAAATAACCGGAAACCGGCGGCAAAATCTTGGCAGTATCCTTATGGAATTACTTTTCCCGGTAAACCCACCGGCCGTTTCTCCGATGGCCGTGTTTTGACCGATTACCTAGCCAAGTATCTGAGAGTGAAGTCTCCGATTGCGTACAAATGGCGGAAAGTAGGGTTAGGGCAACTGAAATACGGGATGAATTTCGCGTACGGCGGTACCGGCGTTTTCAATACCTTTATAATGAGCCCTAACATGAGCACTCAGATCGACTTCCTCCAGCAGCTGATCCACGAATCGACGTATACCGCCCAAGATTTGCATTACTCTGTGGCGCTGATCTCCCTCGCCGGCAACGATTATGGCGTCTACCAGGCCACTGACGGCTCTGCTCAGGGGTGGCAGCCATTTATAACGAAAGTGGTGAACCAGCTGGAGGTGAATTTGAGGCGCATCCATGGGCTGGGGGTTCCAAAGATAGTGGTGACGTCTCTGGAGCCGCTGGGCTGTCTTCCTAGCAGCACAGTGGCATCCTCATTCCATCAGTGTAATGCCACTGAGAATCTGCTTGTCAACTTCCACAATCTGCTATTGCAGCAATCTGTGGCAAAGCTCAACAGTGAAGTAAAAGATGCCTCTGCATCCACTTTCATCCTTCTTGATCTGTATTCCTCTTTCATGGCTGCTCTCAACAACAAAGGAGACCCTCTTGGGAACGTGAAATTTGAGAACCCATTGAAGCCATGTTGTGTTGGAATAAGCAGCGAGTATGCATGTGGGAATGTGGGTGCGAATGGGGAAAAGAAGTACACCATATGTGAAGATCCAGGAGCTGCATTCTTCTGGGACGAAGTGCACCCTTCTCAAAATGGCTGGTTCGCTGTGTACTCAGCATTGCAAGCAAATTTAAAGCAACTCTAAGCTTTAAATTACATAGAAATCAGATAAGTGACCCCCCAATTTGACAAGACATTTCATTCATTCAATGATCATCTGTCTTGTAGTATTCAAATAATATTTGGCATAGCCCCTTTGCCTCTGCTCTCTGATTTGCTTGTAGGTATTCATCTTTCTCGCCCCNNNNNN

Coding sequence (CDS)

ATGGACTGCAAAAAGCTCCTCTGTTTCTCTCTCCTCTGCTTCTCTCTCCTCCCAGAATTATATGGGGTGGTTTGGGTTTCTGTTGGAGGGGGTGGCCACCGTCATCGCCACCGCGTGCGGCGGAGTGGACAGAGTCTTGGTGGAGGGGAGCTGTCGGATTCGAATCCTAGAAAGCTGTTTGTGTTTGGGGATTCTTATGTAGACACCGGAAATAACCGGAAACCGGCGGCAAAATCTTGGCAGTATCCTTATGGAATTACTTTTCCCGGTAAACCCACCGGCCGTTTCTCCGATGGCCGTGTTTTGACCGATTACCTAGCCAAGTATCTGAGAGTGAAGTCTCCGATTGCGTACAAATGGCGGAAAGTAGGGTTAGGGCAACTGAAATACGGGATGAATTTCGCGTACGGCGGTACCGGCGTTTTCAATACCTTTATAATGAGCCCTAACATGAGCACTCAGATCGACTTCCTCCAGCAGCTGATCCACGAATCGACGTATACCGCCCAAGATTTGCATTACTCTGTGGCGCTGATCTCCCTCGCCGGCAACGATTATGGCGTCTACCAGGCCACTGACGGCTCTGCTCAGGGGTGGCAGCCATTTATAACGAAAGTGGTGAACCAGCTGGAGGTGAATTTGAGGCGCATCCATGGGCTGGGGGTTCCAAAGATAGTGGTGACGTCTCTGGAGCCGCTGGGCTGTCTTCCTAGCAGCACAGTGGCATCCTCATTCCATCAGTGTAATGCCACTGAGAATCTGCTTGTCAACTTCCACAATCTGCTATTGCAGCAATCTGTGGCAAAGCTCAACAGTGAAGTAAAAGATGCCTCTGCATCCACTTTCATCCTTCTTGATCTGTATTCCTCTTTCATGGCTGCTCTCAACAACAAAGGAGACCCTCTTGGGAACGTGAAATTTGAGAACCCATTGAAGCCATGTTGTGTTGGAATAAGCAGCGAGTATGCATGTGGGAATGTGGGTGCGAATGGGGAAAAGAAGTACACCATATGTGAAGATCCAGGAGCTGCATTCTTCTGGGACGAAGTGCACCCTTCTCAAAATGGCTGGTTCGCTGTGTACTCAGCATTGCAAGCAAATTTAAAGCAACTCTAA

Protein sequence

MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Homology
BLAST of CmoCh06G004660 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.1e-104
Identity = 195/370 (52.70%), Postives = 244/370 (65.95%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60
           MD    L F L  F     L+G +    G   + H +  R               P KLF
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFR---------------PTKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSY DTGN +K  + SW++PYGITFPGKP GRFSDGRV TD+LAK++ +KSPI Y W
Sbjct: 61  VFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFW 120

Query: 121 RK-VGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHE-STYTAQDLHYSVAL 180
           +   G  +L+YGMNFAYGGTGVFNT    PNM+TQID  Q ++     Y   +L  SVAL
Sbjct: 121 KDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVAL 180

Query: 181 ISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPS 240
           +S+AGNDY  + A +  A  +  FI +VV+Q EVNLRRIH LGV KI V SL+PLGCLP 
Sbjct: 181 VSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPP 240

Query: 241 STVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNK 300
            T  +SF +CN T+N LVN HN LLQQ VAKLN+E K    STFI+LDLY++F+    NK
Sbjct: 241 FTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQ---STFIILDLYNAFLTVFKNK 300

Query: 301 GDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWF 360
           G   G+ +FE+PLKPCCVG+S EY CG+V   G KKY +C++P  AFFWD +HP++ GW 
Sbjct: 301 GSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWR 352

Query: 361 AVYSALQANL 369
           +VYS L+ +L
Sbjct: 361 SVYSVLRESL 352

BLAST of CmoCh06G004660 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.9e-99
Identity = 177/319 (55.49%), Postives = 225/319 (70.53%), Query Frame = 0

Query: 56  PRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSP 115
           P +LFVFGDSY DTGN RK  + SW+ PYGITFP KP+GRFSDGRV TD+LA+YL +KSP
Sbjct: 37  PNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSP 96

Query: 116 IAYKWRK-VGLGQLKYGMNFAYGGTGVFNTFIMS-PNMSTQIDFLQQLIHE-STYTAQDL 175
           I Y W+   G  +L YGMN+AYGGTGVF T     PNM+TQID+ Q+++   + Y+  DL
Sbjct: 97  IPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDL 156

Query: 176 HYSVALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEP 235
             S+AL+S+AGNDY  + A          F+ +VV+Q+ VN  RIH LGV KIV+ S++P
Sbjct: 157 PSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQP 216

Query: 236 LGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFM 295
           LGCLPS TV +SF +CNAT+N   N HN LL +++A+LN+E K    STF++LD Y++F+
Sbjct: 217 LGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETK---PSTFVVLDHYNAFL 276

Query: 296 AALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHP 355
               NKG   G  +F NPLKPCCVG++S Y C NV   GEKKY ICEDP AAFFWD  HP
Sbjct: 277 TVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHP 336

Query: 356 SQNGWFAVYSALQANLKQL 372
           S+ GW +VYS L  +LK +
Sbjct: 337 SEEGWRSVYSVLHKHLKAI 352

BLAST of CmoCh06G004660 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-72
Identity = 153/333 (45.95%), Postives = 208/333 (62.46%), Query Frame = 0

Query: 45  SLGGGELSDSNPR-KLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLT 104
           ++G  + S +N   KLFVFG+SY DTG N KP A SW+ PYGITFPGKP+GR+SDG   T
Sbjct: 20  AVGSNQPSQANKNPKLFVFGNSYADTG-NMKPTALSWKLPYGITFPGKPSGRYSDGLTAT 79

Query: 105 DYLAKYLRVKSPIAYKWRKVGLGQLKY--GMNFAYGGTGVFNTFI-MSPNMSTQIDFLQQ 164
           D+LAK L  K P  Y WR  G  ++K   GMNFA+GG+ VF++ +  SPN+STQ+ FL  
Sbjct: 80  DFLAKQLGAKLP--YLWRTHGKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVN 139

Query: 165 L-IHESTYTAQ-DLHYSVALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIH 224
           L +    YT   DL  S AL+S +G DY  +   + +   +  F+  +V  ++ +L  ++
Sbjct: 140 LALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMN 199

Query: 225 GLGVPKIVVTSLEPLGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVK-DA 284
           GL    I VTSL PLGCLP  TVASSF  CN + + LV  HN  L+++VAKLN E K   
Sbjct: 200 GLKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNKEDKFRT 259

Query: 285 SASTFILLDLYSSFMAALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTI 344
               F+++DL+ +FM  L  K    GN +F++PLKPCC G      C  +   G KKYT+
Sbjct: 260 KGDRFVIVDLHKAFMTILEKK----GNKRFKSPLKPCCEG-----DCARMDMKGAKKYTL 319

Query: 345 CEDPGAAFFWDEVHPSQNGWFAVYSALQANLKQ 371
           C DP +AFFWDE++P+Q GW ++YS L  +L +
Sbjct: 320 CNDPKSAFFWDEINPTQEGWRSIYSLLGKSLTE 340

BLAST of CmoCh06G004660 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 271.6 bits (693), Expect = 1.4e-71
Identity = 144/312 (46.15%), Postives = 189/312 (60.58%), Query Frame = 0

Query: 56  PRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSP 115
           P KLFVFGDSY DTGN       SW++P GITFPG PTGRFSDGRV TDYLAKY+ V++P
Sbjct: 45  PTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTP 104

Query: 116 IAYKWRKVGLGQL--KYGMNFAYGGTGVFNT-FIMSPNMSTQIDFLQQLIHESTYTAQDL 175
           I YKW K G  +L  K GMNFAYGG G F T F + P  S QID  +QL+  + Y+  DL
Sbjct: 105 ITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSPADL 164

Query: 176 HYSVALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEP 235
           + SVA  S+ GNDY  Y   +GS +G      KVV Q+ ++++RI  LGV K++V    P
Sbjct: 165 NSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALSPP 224

Query: 236 LGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFM 295
             CLP          C+ T +     HN LL++ + KLN +  + +  +F+ LDLY++F+
Sbjct: 225 QKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYNAFV 284

Query: 296 AALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHP 355
               NKG   G   F +P K CC      + CG+   +G+K YT+C+DP + FFWD VH 
Sbjct: 285 TIFKNKG-VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDNVHI 344

Query: 356 SQNGWFAVYSAL 365
           S  GW +V+S L
Sbjct: 345 SDQGWRSVFSLL 350

BLAST of CmoCh06G004660 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.3e-68
Identity = 138/310 (44.52%), Postives = 193/310 (62.26%), Query Frame = 0

Query: 58  KLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIA 117
           KLFVFGDSY DTGN ++   ++W  PYGITFPGKP+GR+ DG + TD+L K L  +SP  
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 118 YKWRKVGLGQLKYGMNFAYGGTGVFNTFIMS--PNMSTQIDFLQQLIHESTYTAQDLHYS 177
           Y+      G LK GMNFA+GG+ + ++   S  PN++ Q++FL  L+             
Sbjct: 74  YRTHGRDKG-LKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITPSD 133

Query: 178 VALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGC 237
           V+LIS AG DY  Y   +  A G +  + KVV+ L VN+  + GL   KI VTSL+P+GC
Sbjct: 134 VSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQPIGC 193

Query: 238 LPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASAST-FILLDLYSSFMAA 297
           LPS T ASSF  CN +++ LV  HN LL++ VAKLN + +       F ++D++++FM  
Sbjct: 194 LPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNAFMTV 253

Query: 298 LNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQ 357
           + NK    G+ +F+NP+K CC G      CG   ++G K YT+C+DP + FFWD VHP+Q
Sbjct: 254 MKNK----GSKRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDPKSFFFWDAVHPTQ 311

Query: 358 NGWFAVYSAL 365
            GW ++YS L
Sbjct: 314 EGWRSIYSVL 311

BLAST of CmoCh06G004660 vs. ExPASy TrEMBL
Match: A0A6J1EEL4 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111433403 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.0e-218
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60
           MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF
Sbjct: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST 240
           LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST
Sbjct: 181 LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST 240

Query: 241 VASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300
           VASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD
Sbjct: 241 VASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300

Query: 301 PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAV 360
           PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAV
Sbjct: 301 PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAV 360

Query: 361 YSALQANLKQL 372
           YSALQANLKQL
Sbjct: 361 YSALQANLKQL 371

BLAST of CmoCh06G004660 vs. ExPASy TrEMBL
Match: A0A6J1L3E4 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111498754 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 5.7e-214
Identity = 365/371 (98.38%), Postives = 366/371 (98.65%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60
           MD KKLLCFSLLCFSLLPELYGVVWVS GG GHRHRHRVRRSGQ LGGGELSDSNPRKLF
Sbjct: 1   MDSKKLLCFSLLCFSLLPELYGVVWVSAGGVGHRHRHRVRRSGQRLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST 240
           LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST
Sbjct: 181 LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST 240

Query: 241 VASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300
           VASSF QCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD
Sbjct: 241 VASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300

Query: 301 PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAV 360
           PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICE+PGAAFFWDEVHPSQNGWFAV
Sbjct: 301 PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICENPGAAFFWDEVHPSQNGWFAV 360

Query: 361 YSALQANLKQL 372
           YSALQANLKQL
Sbjct: 361 YSALQANLKQL 371

BLAST of CmoCh06G004660 vs. ExPASy TrEMBL
Match: A0A6J1J6I3 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111481670 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.7e-195
Identity = 331/370 (89.46%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60
           MD K LLCFS+LCFSLLPEL GVV VS G G HRHRHR RRS QSLGGGEL +SNPRKLF
Sbjct: 1   MDSKSLLCFSILCFSLLPELSGVVRVSAGRGSHRHRHRERRSSQSLGGGELWNSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYG+TFPGKPTGRFSDGRVLTDYLAKYL+VKSPI YKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGVTFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVG GQLKYGMNFAYGGTGVFNTF+MSPNMSTQIDF QQLI E T+T+QDL  SVAL+S
Sbjct: 121 RKVGYGQLKYGMNFAYGGTGVFNTFVMSPNMSTQIDFFQQLIGELTFTSQDLQSSVALVS 180

Query: 181 LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST 240
           LAGNDY VYQATDGSAQGWQPFITKVVNQLEVNLRRIHG+GVPKIVVT+LEPLGCLPSST
Sbjct: 181 LAGNDYSVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGVGVPKIVVTALEPLGCLPSST 240

Query: 241 VASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300
           VASSF QCNATEN LVNFHN+LLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD
Sbjct: 241 VASSFQQCNATENQLVNFHNMLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300

Query: 301 PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAV 360
            LGNVKFENPLKPCCVG+SSEY+CG++G+NGEKKYTICEDPGAAFFWDEVHP+Q+GWFAV
Sbjct: 301 HLGNVKFENPLKPCCVGVSSEYSCGSMGSNGEKKYTICEDPGAAFFWDEVHPTQHGWFAV 360

Query: 361 YSALQANLKQ 371
           YSALQANLKQ
Sbjct: 361 YSALQANLKQ 370

BLAST of CmoCh06G004660 vs. ExPASy TrEMBL
Match: A0A6J1FAT3 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111442352 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.0e-194
Identity = 330/370 (89.19%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60
           MD K LLCFS+LCFSLLPEL GVV VS G   HRHRHR RRS QSLGGGEL +SNPRKLF
Sbjct: 1   MDSKTLLCFSILCFSLLPELSGVVRVSAGRVSHRHRHRERRSSQSLGGGELWNSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYG+TFPGKPTGRFSDGRVLTDYLAKYL+VKSPI YKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGVTFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVG GQLKYGMNFAYGGTGVFNTF+MSPNMSTQIDF QQLI EST+T+QDL  SVAL+S
Sbjct: 121 RKVGYGQLKYGMNFAYGGTGVFNTFVMSPNMSTQIDFFQQLIGESTFTSQDLQSSVALVS 180

Query: 181 LAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSST 240
           LAGNDY VYQATDGSAQGWQPFITKVVNQLEVNLRRIHG+GVPKIVVT+LEPLGCLPSST
Sbjct: 181 LAGNDYSVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGVGVPKIVVTALEPLGCLPSST 240

Query: 241 VASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300
           VASSF QCNATEN LVNFHN+LLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD
Sbjct: 241 VASSFQQCNATENQLVNFHNMLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNKGD 300

Query: 301 PLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAV 360
            LGNVKFENPLKPCCVG+SSEY+CG++G+NGEKKYTIC+DPGAAFFWDEVHP+Q+GWFAV
Sbjct: 301 HLGNVKFENPLKPCCVGVSSEYSCGSMGSNGEKKYTICDDPGAAFFWDEVHPTQHGWFAV 360

Query: 361 YSALQANLKQ 371
           YSALQANLKQ
Sbjct: 361 YSALQANLKQ 370

BLAST of CmoCh06G004660 vs. ExPASy TrEMBL
Match: A0A1S3B7C0 (GDSL esterase/lipase At5g03610 OS=Cucumis melo OX=3656 GN=LOC103486596 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.8e-188
Identity = 327/373 (87.67%), Postives = 344/373 (92.23%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60
           MD KKLL   LLCFSLLPELYGVVWVS GGG HR + R     QSLGGGELSDSNPRK+F
Sbjct: 1   MDSKKLLSLYLLCFSLLPELYGVVWVSAGGGAHREQRR----SQSLGGGELSDSNPRKMF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYL+VKSPI YKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKW 120

Query: 121 RKV--GLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVAL 180
           RKV  GLGQLKYGMNFAYGGTGVFNTF+ SPNMSTQIDFLQQL+ EST+T +DL  SVAL
Sbjct: 121 RKVGFGLGQLKYGMNFAYGGTGVFNTFVTSPNMSTQIDFLQQLVGESTFTGRDLLSSVAL 180

Query: 181 ISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPS 240
           +SLAGNDY VYQAT+GS QGWQPFITKVVNQLEVNLRRIHGLGVPKIVVT+LEPLGCLPS
Sbjct: 181 VSLAGNDYSVYQATNGSPQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTALEPLGCLPS 240

Query: 241 STVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNK 300
           +TVASSF QCN+T+N LVNFHNLLLQQSVAKLNSEVKD SASTFILLDLYSSFMAALNNK
Sbjct: 241 TTVASSFQQCNSTQNQLVNFHNLLLQQSVAKLNSEVKDPSASTFILLDLYSSFMAALNNK 300

Query: 301 GDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWF 360
            D LGNVKFENP+KPCCVGISSEYACG+VGANGEKKYTICEDPGAAFFWDEVHP+Q GW+
Sbjct: 301 VDQLGNVKFENPMKPCCVGISSEYACGSVGANGEKKYTICEDPGAAFFWDEVHPTQYGWY 360

Query: 361 AVYSALQANLKQL 372
           AVYSALQANLKQL
Sbjct: 361 AVYSALQANLKQL 369

BLAST of CmoCh06G004660 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-105
Identity = 195/370 (52.70%), Postives = 244/370 (65.95%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60
           MD    L F L  F     L+G +    G   + H +  R               P KLF
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFR---------------PTKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSY DTGN +K  + SW++PYGITFPGKP GRFSDGRV TD+LAK++ +KSPI Y W
Sbjct: 61  VFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFW 120

Query: 121 RK-VGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHE-STYTAQDLHYSVAL 180
           +   G  +L+YGMNFAYGGTGVFNT    PNM+TQID  Q ++     Y   +L  SVAL
Sbjct: 121 KDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVAL 180

Query: 181 ISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPS 240
           +S+AGNDY  + A +  A  +  FI +VV+Q EVNLRRIH LGV KI V SL+PLGCLP 
Sbjct: 181 VSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPP 240

Query: 241 STVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFMAALNNK 300
            T  +SF +CN T+N LVN HN LLQQ VAKLN+E K    STFI+LDLY++F+    NK
Sbjct: 241 FTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQ---STFIILDLYNAFLTVFKNK 300

Query: 301 GDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWF 360
           G   G+ +FE+PLKPCCVG+S EY CG+V   G KKY +C++P  AFFWD +HP++ GW 
Sbjct: 301 GSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWR 352

Query: 361 AVYSALQANL 369
           +VYS L+ +L
Sbjct: 361 SVYSVLRESL 352

BLAST of CmoCh06G004660 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 4.2e-100
Identity = 177/319 (55.49%), Postives = 225/319 (70.53%), Query Frame = 0

Query: 56  PRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSP 115
           P +LFVFGDSY DTGN RK  + SW+ PYGITFP KP+GRFSDGRV TD+LA+YL +KSP
Sbjct: 37  PNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSP 96

Query: 116 IAYKWRK-VGLGQLKYGMNFAYGGTGVFNTFIMS-PNMSTQIDFLQQLIHE-STYTAQDL 175
           I Y W+   G  +L YGMN+AYGGTGVF T     PNM+TQID+ Q+++   + Y+  DL
Sbjct: 97  IPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDL 156

Query: 176 HYSVALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEP 235
             S+AL+S+AGNDY  + A          F+ +VV+Q+ VN  RIH LGV KIV+ S++P
Sbjct: 157 PSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQP 216

Query: 236 LGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFM 295
           LGCLPS TV +SF +CNAT+N   N HN LL +++A+LN+E K    STF++LD Y++F+
Sbjct: 217 LGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETK---PSTFVVLDHYNAFL 276

Query: 296 AALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHP 355
               NKG   G  +F NPLKPCCVG++S Y C NV   GEKKY ICEDP AAFFWD  HP
Sbjct: 277 TVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHP 336

Query: 356 SQNGWFAVYSALQANLKQL 372
           S+ GW +VYS L  +LK +
Sbjct: 337 SEEGWRSVYSVLHKHLKAI 352

BLAST of CmoCh06G004660 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 9.7e-73
Identity = 144/312 (46.15%), Postives = 189/312 (60.58%), Query Frame = 0

Query: 56  PRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSP 115
           P KLFVFGDSY DTGN       SW++P GITFPG PTGRFSDGRV TDYLAKY+ V++P
Sbjct: 45  PTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTP 104

Query: 116 IAYKWRKVGLGQL--KYGMNFAYGGTGVFNT-FIMSPNMSTQIDFLQQLIHESTYTAQDL 175
           I YKW K G  +L  K GMNFAYGG G F T F + P  S QID  +QL+  + Y+  DL
Sbjct: 105 ITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSPADL 164

Query: 176 HYSVALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEP 235
           + SVA  S+ GNDY  Y   +GS +G      KVV Q+ ++++RI  LGV K++V    P
Sbjct: 165 NSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVALSPP 224

Query: 236 LGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASASTFILLDLYSSFM 295
             CLP          C+ T +     HN LL++ + KLN +  + +  +F+ LDLY++F+
Sbjct: 225 QKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYNAFV 284

Query: 296 AALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHP 355
               NKG   G   F +P K CC      + CG+   +G+K YT+C+DP + FFWD VH 
Sbjct: 285 TIFKNKG-VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDNVHI 344

Query: 356 SQNGWFAVYSAL 365
           S  GW +V+S L
Sbjct: 345 SDQGWRSVFSLL 350

BLAST of CmoCh06G004660 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.8e-69
Identity = 138/310 (44.52%), Postives = 193/310 (62.26%), Query Frame = 0

Query: 58  KLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIA 117
           KLFVFGDSY DTGN ++   ++W  PYGITFPGKP+GR+ DG + TD+L K L  +SP  
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 118 YKWRKVGLGQLKYGMNFAYGGTGVFNTFIMS--PNMSTQIDFLQQLIHESTYTAQDLHYS 177
           Y+      G LK GMNFA+GG+ + ++   S  PN++ Q++FL  L+             
Sbjct: 74  YRTHGRDKG-LKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITPSD 133

Query: 178 VALISLAGNDYGVYQATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGC 237
           V+LIS AG DY  Y   +  A G +  + KVV+ L VN+  + GL   KI VTSL+P+GC
Sbjct: 134 VSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQPIGC 193

Query: 238 LPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDASAST-FILLDLYSSFMAA 297
           LPS T ASSF  CN +++ LV  HN LL++ VAKLN + +       F ++D++++FM  
Sbjct: 194 LPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNAFMTV 253

Query: 298 LNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQ 357
           + NK    G+ +F+NP+K CC G      CG   ++G K YT+C+DP + FFWD VHP+Q
Sbjct: 254 MKNK----GSKRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDPKSFFFWDAVHPTQ 311

Query: 358 NGWFAVYSAL 365
            GW ++YS L
Sbjct: 314 EGWRSIYSVL 311

BLAST of CmoCh06G004660 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-39
Identity = 102/301 (33.89%), Postives = 145/301 (48.17%), Query Frame = 0

Query: 74  KPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQLKYGMN 133
           KP A SW+ PYGITFPGKP+GR+SDG   TD+L                           
Sbjct: 2   KPTALSWKLPYGITFPGKPSGRYSDGLTATDFL--------------------------- 61

Query: 134 FAYGGTGVFNTFI-MSPNMSTQIDFLQQL-IHESTYTAQ-DLHYSVALISLAGNDYGVYQ 193
               G+ VF++ +  SPN+STQ+ FL  L +    YT   DL  S AL+S +G DY  + 
Sbjct: 62  ----GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFI 121

Query: 194 ATDGSAQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFHQCNA 253
             + +   +  F+  +V  +                                     CN 
Sbjct: 122 DQNPNMAAYPAFVEFIVEDI------------------------------------HCNE 181

Query: 254 TENLLVNFHNLLLQQSVAKLNSEVK-DASASTFILLDLYSSFMAALNNKGDPLGNVKFEN 313
           + + LV  HN  L+++VAKLN E K       F+++DL+ +FM  L  K    GN +F++
Sbjct: 182 SYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKK----GNKRFKS 226

Query: 314 PLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQANLK 371
           PLKPCC G      C  +   G KKYT+C DP +AFFWDE++P+Q GW ++YS L  +L 
Sbjct: 242 PLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSLT 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZS72.1e-10452.70GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2... [more]
Q9SF945.9e-9955.49GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2... [more]
Q9LZS91.2e-7245.95GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3... [more]
Q8RWJ41.4e-7146.15GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2... [more]
Q9LZS85.3e-6844.52GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1EEL41.0e-218100.00GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC11143340... [more]
A0A6J1L3E45.7e-21498.38GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111498754 ... [more]
A0A6J1J6I37.7e-19589.46GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111481670 ... [more]
A0A6J1FAT35.0e-19489.19GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC11144235... [more]
A0A1S3B7C01.8e-18887.67GDSL esterase/lipase At5g03610 OS=Cucumis melo OX=3656 GN=LOC103486596 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G03610.11.5e-10552.70GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G09930.14.2e-10055.49GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G36325.19.7e-7346.15GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03600.13.8e-6944.52SGNH hydrolase-type esterase superfamily protein [more]
AT5G03590.11.1e-3933.89GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 52..370
e-value: 3.1E-55
score: 189.8
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 59..356
e-value: 4.9E-25
score: 89.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..52
NoneNo IPR availablePANTHERPTHR46020OSJNBB0059K02.9 PROTEINcoord: 54..366
NoneNo IPR availablePANTHERPTHR46020:SF17GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 54..366
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 58..369

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G004660.1CmoCh06G004660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds