CmoCh06G002830 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh06G002830
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionheparanase-like protein 1
LocationCmo_Chr06: 1415073 .. 1417381 (+)
RNA-Seq ExpressionCmoCh06G002830
SyntenyCmoCh06G002830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATACCAAATCCTTTTGATTTGTGTTCTTGCTTTCGTCCCAACCATTTTAGGTCGTGGCACAATCATTTTGGGTCATGACACAACAATGGGTACAATTGTGATTGATGGAACCAAAACGATAGCAGAAACAGATGAAAATTTCGTTTGCTTAACTTTAGACATTTGGCCTCATGACGAGTGTCGTTGGTCCAAACTTTGTGTTTGGGATGGTCATGCATCGATGCTCAACTTGGTGAGAATATACATGTTCATCAGAAAAAAATACAATTAATAGTATTTTATTAATCAATTTTTTTTTTTCTTTATTGTTCCAGGATTTAACTCTTCCTATCCTTAACAAAGCAGTTCAAGGTTTGACTGTTTGTAATGCATCGATAATCTTTTTATAGCATATTGTTTCTTCTAAAAATTTACCTTAATTTGCTCTCTATCATTAGCTTTCAAGTCGGTAAGGATTAGAGTAGGAGGTACTTTGCAAGACAAATTGATCTATAACGTCGGGGCTGGTTTCAAGGGAACCTGTCACCCATTTCAAGCAATCGTAGGTTCGTTATTTGACTTCTCAGTGGGATGTTTGTATATGGAACGGTGGGATGATTTGAACAACTTTTTCAATAATACCGGGTATGTTCTCTTTATTTTATTAATTTTTCTTGCTTTCTTTTCCCCTTTTGAATACTGAAACTTCTTTTAATCCGACGACAGAGCGATTGTGACTTTTGGCTTGAATGCTCTGTTGGGTAAGCACAATACAAAAGGAATTCAATGGGAAGGAAAATGGAATTATAGCAATGCCGAGGCTCTTATTCAATACACCGTAGAGAAGAATTATAAAATAAATTCATGGGAGTTCGGTAAGCTAACTAATACTAGAAGTCAATTGTTCTCGGTTGATTTAATGACCATAATTCATGCGTACTGAGGCATATGCCACCACTACACAGGCAATGAATTGGTGGGACGTATTAGTATTGGTGCAAGTATTACTGCAGCACAATACGCAGAAGATCTTCTCAAGCTTCGAGAAATCATAGATCGTTTGTATAATAACTCCCAACAAAAACCTCTGATTGTTGCACCAGGTGCATTCTTTGATGAGGCATGGTATGATGAATTTGTTAGAAAAACTGGACCAAAAGCTGTCGATGTTCTCACTCATCATATATATAACATGGGTGCAGGTTTGACTACTGTTCTTGCTACCATTCTCGTTATATGTATCCAAACTGTAATATGAGTATTGAGAACCTGTTTTTGGTTTGTAGGTCATGACCCCAAATTGATTTACAGATTCGTTAATTCTACGTATCTTTCTCAAGTATCAAACACATTTGAACAACTCGAGAATGTAATTCAAAAGAATGCCCCGTGGGCTGCGGCTTGGGTTGGAGAAGCGGGTGGAGCCTATCAGGGTGGAAGTCCTCATCTTTCCGATACATTTATCAATAGTTTTTGGTAAACAACTTTGGTTCACCTTTTTTTTTCCTTATTAGGATTTTCCCCATTTGAATATGAAAGAAAAAAGAATGCTCGGGTTTAAAGAATGACTTGAATGTATTAGGTATTTGGATCAGCTTGGGATGGCTTCTTTGTACAATACGAAAGTATATTGTAGGCAGACTTTGATCGGTGGATTCTATGGCGTCCTCAAATCCTCAACTTTACTTCCTACACCAGATTACTACGGGTAATCAAATATCTCTTCTTCTTCTATCTTTTGATCATAATATTTATACAAACTCTCACTTCCTTTGGTATTTTTTATTAGTGCACTTCTCTTTCACCGACTTATGGGGCCGGGAGTTCTCAAAATTGACAATAAGGTGTCTTCTGACCTTCGTAGCTACGCTCATTGCTCAAGAGGAAGAGTAAGAAGTCATTTTTAGAGTTTAATATAATAAACTTTTATTTGATATCATCCATTGATTCTAATTTATTATTTTCGTGTAGTCGGGTGTGACCTTACTTCTCATCAATTTGAGCAACACAACTAACTTTTTAATCACCATTCAAAATGACATGAACATGAGCTTGCGTAAAAGCAATACTCGGACAAATGATTCTCATTTAAAGGATAGACAAAAAAGAACAGCTTTAAGAGAAGAATATCATTTAACTCCAAAAGATGGTCTACTTCGAAGCTCCACGGTACTCTTAAATGGAAATCCATTGGAAACTACAAAAGAGGGAGACATACCAAATCTTGTACCCGTCTACCGTCAGAGCAACTCTTATATACATATTGCTAGTTGGTCCATTGCTTTTGTTGTCCTTCCTGACTTTGTAGCTCCCGCCTGCAAGTGA

mRNA sequence

ATGGGATACCAAATCCTTTTGATTTGTGTTCTTGCTTTCGTCCCAACCATTTTAGGTCGTGGCACAATCATTTTGGGTCATGACACAACAATGGGTACAATTGTGATTGATGGAACCAAAACGATAGCAGAAACAGATGAAAATTTCGTTTGCTTAACTTTAGACATTTGGCCTCATGACGAGTGTCGTTGGTCCAAACTTTGTGTTTGGGATGGTCATGCATCGATGCTCAACTTGGATTTAACTCTTCCTATCCTTAACAAAGCAGTTCAAGCTTTCAAGTCGGTAAGGATTAGAGTAGGAGGTACTTTGCAAGACAAATTGATCTATAACGTCGGGGCTGGTTTCAAGGGAACCTGTCACCCATTTCAAGCAATCGTAGGTTCGTTATTTGACTTCTCAGTGGGATGTTTGTATATGGAACGGTGGGATGATTTGAACAACTTTTTCAATAATACCGGAGCGATTGTGACTTTTGGCTTGAATGCTCTGTTGGGTAAGCACAATACAAAAGGAATTCAATGGGAAGGAAAATGGAATTATAGCAATGCCGAGGCTCTTATTCAATACACCGTAGAGAAGAATTATAAAATAAATTCATGGGAGTTCGGCATATGCCACCACTACACAGGCAATGAATTGGTGGGACGTATTAGTATTGGTGCAAGTATTACTGCAGCACAATACGCAGAAGATCTTCTCAAGCTTCGAGAAATCATAGATCGTTTGTATAATAACTCCCAACAAAAACCTCTGATTGTTGCACCAGGTGCATTCTTTGATGAGGCATGGTATGATGAATTTGTTAGAAAAACTGGACCAAAAGCTGTCGATGTTCTCACTCATCATATATATAACATGGGTGCAGGTCATGACCCCAAATTGATTTACAGATTCGTTAATTCTACGTATCTTTCTCAAGTATCAAACACATTTGAACAACTCGAGAATGTAATTCAAAAGAATGCCCCGTGGGCTGCGGCTTGGGTTGGAGAAGCGGGTGGAGCCTATCAGGGTGGAAGTCCTCATCTTTCCGATACATTTATCAATAGATTTTCCCCATTTGAATATGAAAGAAAAAAGAATGCTCGGCTTGGGATGGCTTCTTTGTACAATACGAAAGTATATTGTAGGCAGACTTTGATCGGTGGATTCTATGGCGTCCTCAAATCCTCAACTTTACTTCCTACACCAGATTACTACGGTGCACTTCTCTTTCACCGACTTATGGGGCCGGGAGTTCTCAAAATTGACAATAAGGTGTCTTCTGACCTTCGTAGCTACGCTCATTGCTCAAGAGGAAGATCGGGTGTGACCTTACTTCTCATCAATTTGAGCAACACAACTAACTTTTTAATCACCATTCAAAATGACATGAACATGAGCTTGCGTAAAAGCAATACTCGGACAAATGATTCTCATTTAAAGGATAGACAAAAAAGAACAGCTTTAAGAGAAGAATATCATTTAACTCCAAAAGATGGTCTACTTCGAAGCTCCACGGTACTCTTAAATGGAAATCCATTGGAAACTACAAAAGAGGGAGACATACCAAATCTTGTACCCGTCTACCGTCAGAGCAACTCTTATATACATATTGCTAGTTGGTCCATTGCTTTTGTTGTCCTTCCTGACTTTGTAGCTCCCGCCTGCAAGTGA

Coding sequence (CDS)

ATGGGATACCAAATCCTTTTGATTTGTGTTCTTGCTTTCGTCCCAACCATTTTAGGTCGTGGCACAATCATTTTGGGTCATGACACAACAATGGGTACAATTGTGATTGATGGAACCAAAACGATAGCAGAAACAGATGAAAATTTCGTTTGCTTAACTTTAGACATTTGGCCTCATGACGAGTGTCGTTGGTCCAAACTTTGTGTTTGGGATGGTCATGCATCGATGCTCAACTTGGATTTAACTCTTCCTATCCTTAACAAAGCAGTTCAAGCTTTCAAGTCGGTAAGGATTAGAGTAGGAGGTACTTTGCAAGACAAATTGATCTATAACGTCGGGGCTGGTTTCAAGGGAACCTGTCACCCATTTCAAGCAATCGTAGGTTCGTTATTTGACTTCTCAGTGGGATGTTTGTATATGGAACGGTGGGATGATTTGAACAACTTTTTCAATAATACCGGAGCGATTGTGACTTTTGGCTTGAATGCTCTGTTGGGTAAGCACAATACAAAAGGAATTCAATGGGAAGGAAAATGGAATTATAGCAATGCCGAGGCTCTTATTCAATACACCGTAGAGAAGAATTATAAAATAAATTCATGGGAGTTCGGCATATGCCACCACTACACAGGCAATGAATTGGTGGGACGTATTAGTATTGGTGCAAGTATTACTGCAGCACAATACGCAGAAGATCTTCTCAAGCTTCGAGAAATCATAGATCGTTTGTATAATAACTCCCAACAAAAACCTCTGATTGTTGCACCAGGTGCATTCTTTGATGAGGCATGGTATGATGAATTTGTTAGAAAAACTGGACCAAAAGCTGTCGATGTTCTCACTCATCATATATATAACATGGGTGCAGGTCATGACCCCAAATTGATTTACAGATTCGTTAATTCTACGTATCTTTCTCAAGTATCAAACACATTTGAACAACTCGAGAATGTAATTCAAAAGAATGCCCCGTGGGCTGCGGCTTGGGTTGGAGAAGCGGGTGGAGCCTATCAGGGTGGAAGTCCTCATCTTTCCGATACATTTATCAATAGATTTTCCCCATTTGAATATGAAAGAAAAAAGAATGCTCGGCTTGGGATGGCTTCTTTGTACAATACGAAAGTATATTGTAGGCAGACTTTGATCGGTGGATTCTATGGCGTCCTCAAATCCTCAACTTTACTTCCTACACCAGATTACTACGGTGCACTTCTCTTTCACCGACTTATGGGGCCGGGAGTTCTCAAAATTGACAATAAGGTGTCTTCTGACCTTCGTAGCTACGCTCATTGCTCAAGAGGAAGATCGGGTGTGACCTTACTTCTCATCAATTTGAGCAACACAACTAACTTTTTAATCACCATTCAAAATGACATGAACATGAGCTTGCGTAAAAGCAATACTCGGACAAATGATTCTCATTTAAAGGATAGACAAAAAAGAACAGCTTTAAGAGAAGAATATCATTTAACTCCAAAAGATGGTCTACTTCGAAGCTCCACGGTACTCTTAAATGGAAATCCATTGGAAACTACAAAAGAGGGAGACATACCAAATCTTGTACCCGTCTACCGTCAGAGCAACTCTTATATACATATTGCTAGTTGGTCCATTGCTTTTGTTGTCCTTCCTGACTTTGTAGCTCCCGCCTGCAAGTGA

Protein sequence

MGYQILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQKRTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAFVVLPDFVAPACK
Homology
BLAST of CmoCh06G002830 vs. ExPASy Swiss-Prot
Match: Q9FF10 (Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 2.9e-155
Identity = 272/564 (48.23%), Postives = 385/564 (68.26%), Query Frame = 0

Query: 1   MGYQILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD 60
           MG+++ +I V  F+  +L      +  +    +IVI G + + ETDENFVC TLD WPHD
Sbjct: 1   MGFRVCVIVV--FLGCLLLVPEKTMAQEMKRASIVIQGARRVCETDENFVCATLDWWPHD 60

Query: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC 120
           +C + + C W G++S++N+DLT P+L KA++AFK +RIR+GG+LQD++IY+VG   K  C
Sbjct: 61  KCNYDQ-CPW-GYSSVINMDLTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVG-NLKTPC 120

Query: 121 HPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180
            PFQ +   LF FS GCL+M+RWD+LN+F   TGA+VTFGLNAL G+H  +G  W G W+
Sbjct: 121 RPFQKMNSGLFGFSKGCLHMKRWDELNSFLTATGAVVTFGLNALRGRHKLRGKAWGGAWD 180

Query: 181 YSNAEALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREII 240
           + N +  + YTV K Y I+SWEF       GNEL G   +GAS++A  Y +DL+ L+++I
Sbjct: 181 HINTQDFLNYTVSKGYVIDSWEF-------GNELSGS-GVGASVSAELYGKDLIVLKDVI 240

Query: 241 DRLYNNS-QQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRF 300
           +++Y NS   KP++VAPG F+++ WY + +  +GP  VDV+THHIYN+G+G+DP L+ + 
Sbjct: 241 NKVYKNSWLHKPILVAPGGFYEQQWYTKLLEISGPSVVDVVTHHIYNLGSGNDPALVKKI 300

Query: 301 VNSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYER 360
           ++ +YLSQVS TF+ +   IQ++ PWA+ WVGE+GGAY  G  H+SDTFI+ F   +   
Sbjct: 301 MDPSYLSQVSKTFKDVNQTIQEHGPWASPWVGESGGAYNSGGRHVSDTFIDSFWYLD--- 360

Query: 361 KKNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDN 420
               +LGM++ +NTKVYCRQTL+GGFYG+L+  T +P PDYY ALL+HRLMG GVL +  
Sbjct: 361 ----QLGMSARHNTKVYCRQTLVGGFYGLLEKGTFVPNPDYYSALLWHRLMGKGVLAVQT 420

Query: 421 KVSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQ 480
                LR YAHCS+GR+GVTLLLINLSN ++F +++ N +N+ L   + +     L D  
Sbjct: 421 DGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVSVSNGINVVLNAESRKKKS--LLDTL 480

Query: 481 KRT------------ALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQS 540
           KR               REEYHLTP++G+LRS T++LNG  L+ T  GDIP+L PV R  
Sbjct: 481 KRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEPVLRSV 540

Query: 541 NSYIHIASWSIAFVVLPDFVAPAC 552
           NS +++   S++F+VLP+F A AC
Sbjct: 541 NSPLNVLPLSMSFIVLPNFDASAC 542

BLAST of CmoCh06G002830 vs. ExPASy Swiss-Prot
Match: Q8L608 (Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.6e-151
Identity = 271/552 (49.09%), Postives = 367/552 (66.49%), Query Frame = 0

Query: 10  VLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCV 69
           V+ F+  +L    +  G +    T+VIDG++ IAETDENF+C TLD WP ++C + + C 
Sbjct: 5   VVVFLSCLLLLPPVTFGSNMERTTLVIDGSRRIAETDENFICATLDWWPPEKCNYDQ-CP 64

Query: 70  WDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGS 129
           W G+AS++NL+L  P+L KA+QAF+++RIR+GG+LQD++IY+VG   K  C  F+     
Sbjct: 65  W-GYASLINLNLASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVG-DLKTPCTQFKKTDDG 124

Query: 130 LFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQ 189
           LF FS GCLYM+RWD++N+FFN TGAIVTFGLNAL G++   G  W G W+++N +  + 
Sbjct: 125 LFGFSEGCLYMKRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMN 184

Query: 190 YTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQ 249
           YTV K Y I+SWEF       GNEL G   I AS++   Y +DL+ L+ +I  +Y NS+ 
Sbjct: 185 YTVSKGYAIDSWEF-------GNELSGS-GIWASVSVELYGKDLIVLKNVIKNVYKNSRT 244

Query: 250 KPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVS 309
           KPL+VAPG FF+E WY E +R +GP  +DVLTHHIYN+G G+DPKL+ + ++  YLS +S
Sbjct: 245 KPLVVAPGGFFEEQWYSELLRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGIS 304

Query: 310 NTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNARLGMAS 369
             F  +   IQ++ PWAAAWVGEAGGA+  G   +S+TFIN F   +       +LG++S
Sbjct: 305 ELFANVNQTIQEHGPWAAAWVGEAGGAFNSGGRQVSETFINSFWYLD-------QLGISS 364

Query: 370 LYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYA 429
            +NTKVYCRQ L+GGFYG+L+  T +P PDYY ALL+HRLMG G+L +    S  LR+Y 
Sbjct: 365 KHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGILGVQTTASEYLRAYV 424

Query: 430 HCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSL------RKSNTRTNDSHLKDRQKRTA 489
           HCS+ R+G+T+LLINLS  T F + + N + + L      RKS   T  S +     + +
Sbjct: 425 HCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKAS 484

Query: 490 ----LREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIA 549
                REEYHL+PKDG LRS  +LLNG PL  T  GDIP L PV     S ++I   SI+
Sbjct: 485 DGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSIS 538

Query: 550 FVVLPDFVAPAC 552
           F+VLP F APAC
Sbjct: 545 FIVLPTFDAPAC 538

BLAST of CmoCh06G002830 vs. ExPASy Swiss-Prot
Match: Q9FZP1 (Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 1.4e-117
Identity = 232/552 (42.03%), Postives = 323/552 (58.51%), Query Frame = 0

Query: 5   ILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHDECRW 64
           +L +CV  F+   +             GT+ + G   +   DE+F+C TLD WP ++C +
Sbjct: 10  VLFLCVFQFLDCTVSSAV------EENGTVFVYGRAAVGTIDEDFICATLDWWPPEKCDY 69

Query: 65  SKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQ 124
              C WD HAS+LNLDL   IL  A++AF  ++IR+GGTLQD +IY      K  C PF 
Sbjct: 70  GS-CSWD-HASILNLDLNNVILQNAIKAFAPLKIRIGGTLQDIVIYET-PDSKQPCLPFT 129

Query: 125 AIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNA 184
                LF ++ GCL M RWD+LN FF  TG  V FGLNAL G+      +  G WNY+NA
Sbjct: 130 KNSSILFGYTQGCLPMRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNA 189

Query: 185 EALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLY 244
           E+ I++T E NY I+ WE        GNEL G   +GA + A QYA D + LR I++R+Y
Sbjct: 190 ESFIRFTAENNYTIDGWEL-------GNELCGS-GVGARVGANQYAIDTINLRNIVNRVY 249

Query: 245 NNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTY 304
            N    PL++ PG FF+  W+ E++ K    +++  T HIY++G G D  LI + +N +Y
Sbjct: 250 KNVSPMPLVIGPGGFFEVDWFTEYLNK-AENSLNATTRHIYDLGPGVDEHLIEKILNPSY 309

Query: 305 LSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNAR 364
           L Q + +F  L+N+I+ ++  A AWVGE+GGAY  G   +S+ F+  F   +       +
Sbjct: 310 LDQEAKSFRSLKNIIKNSSTKAVAWVGESGGAYNSGRNLVSNAFVYSFWYLD-------Q 369

Query: 365 LGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSD 424
           LGMASLY+TK YCRQ+LIGG YG+L ++   P PDYY AL++ +LMG   L      +  
Sbjct: 370 LGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGRKALFTTFSGTKK 429

Query: 425 LRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLR-----KSNTRTNDSHLKDRQ 484
           +RSY HC+R   G+T+LL+NL NTT  +  ++ + + SLR     KS  R + S L    
Sbjct: 430 IRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSYKRAS-SQLFGGP 489

Query: 485 KRTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIA 544
                REEYHLT KDG L S T+LLNGN L+    GD+P + P++  S   I IA +SI 
Sbjct: 490 NGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEPIHINSTEPITIAPYSIV 535

Query: 545 FVVLPDFVAPAC 552
           FV + + V PAC
Sbjct: 550 FVHMRNVVVPAC 535

BLAST of CmoCh06G002830 vs. ExPASy Swiss-Prot
Match: Q9LRC8 (Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 9.0e-96
Identity = 210/561 (37.43%), Postives = 311/561 (55.44%), Query Frame = 0

Query: 1   MGYQILL--ICVLAF-VPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIW 60
           MG+Q+    +CVL F +  I G    ++G +T   TIV      +A+TDEN+VC TLD+W
Sbjct: 1   MGFQVWQKGLCVLCFSLIFICG----VIGEET---TIVKIEENPVAQTDENYVCATLDLW 60

Query: 61  PHDECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFK 120
           P  +C +   C W G +S LNLDL   I+  AV+ F  +++R GGTLQD+L+Y      +
Sbjct: 61  PPTKCNYGN-CPW-GKSSFLNLDLNNNIIRNAVKEFAPLKLRFGGTLQDRLVYQTSRD-E 120

Query: 121 GTCHPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKH-NTKGIQWE 180
                F      + DFS  CL ++RWD++N F   TG+   FGLNAL GK    KGI  +
Sbjct: 121 PCDSTFYNNTNLILDFSHACLSLDRWDEINQFILETGSEAVFGLNALRGKTVEIKGIIKD 180

Query: 181 -----------GKWNYSNAEALIQYTVEKNYK-INSWEFGICHHYTGNELVGRISIGASI 240
                      G+W+YSN++ LI+Y+++K YK I  W         GNEL G  ++   +
Sbjct: 181 GQYLGETTTAVGEWDYSNSKFLIEYSLKKGYKHIRGWTL-------GNELGGH-TLFIGV 240

Query: 241 TAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHI 300
           +   YA D  KL E++  +Y +    PLI+APGA FD  WY EF+ +T    + V THH+
Sbjct: 241 SPEDYANDAKKLHELVKEIYQDQGTMPLIIAPGAIFDLEWYTEFIDRT--PELHVATHHM 300

Query: 301 YNMGAGHDPKLIYRFVNSTYLSQVSNT-FEQLENVIQKNAPWAAAWVGEAGGAYQGGSPH 360
           YN+G+G D  L    + +++  + + + +E L+ ++ +    A AW+GEAGGA+  G   
Sbjct: 301 YNLGSGGDDALKDVLLTASFFDEATKSMYEGLQKIVNRPGTKAVAWIGEAGGAFNSGQDG 360

Query: 361 LSDTFINRFSPFEYERKKNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGA 420
           +S+TFIN F            LG ++L +TK +CRQTL GG YG+L++ T +P PDYY A
Sbjct: 361 ISNTFINGFWYLN-------MLGYSALLDTKTFCRQTLTGGNYGLLQTGTYIPNPDYYSA 420

Query: 421 LLFHRLMGPGVLKIDNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSL 480
           LL+HRLMG  VLK +   + ++  YAHC++  +G+T+L++N    ++  I++        
Sbjct: 421 LLWHRLMGSKVLKTEIVGTKNVYIYAHCAKKSNGITMLVLNHDGESSVKISL-------- 480

Query: 481 RKSNTRTNDSHLKDRQKRTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPV 540
                        D  K  + REEYHLTP +  L+S  V LNG  L     G IP L PV
Sbjct: 481 -------------DPSKYGSKREEYHLTPVNNNLQSRLVKLNGELLHLDPSGVIPALNPV 513

Query: 541 YRQSNSYIHIASWSIAFVVLP 545
            + ++  + +A +S  FV LP
Sbjct: 541 EKDNSKQLEVAPYSFMFVHLP 513

BLAST of CmoCh06G002830 vs. ExPASy Swiss-Prot
Match: X4Y2L4 (Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.6e-31
Identity = 137/536 (25.56%), Postives = 224/536 (41.79%), Query Frame = 0

Query: 34  IVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCVWDGHASMLNLDLTLPILNKAVQAF 93
           + ID    IA   E+F  +       D   +S   +W        +D+T P L K ++  
Sbjct: 6   VTIDDKNVIASVSESFHGVAF-----DASLFSPKGLWS------FVDITSPKLFKLLEGL 65

Query: 94  KSVRIRVGGTLQDKLIYNVGA--------GFKGTCHPFQAIVGSLFDFSVGCLYMERWDD 153
                RVGGT  + L +++           FK        I           L  E +DD
Sbjct: 66  SPGYFRVGGTFANWLFFDLDENNKWKDYWAFKDKTPETATITRRWLFRKQNNLKKETFDD 125

Query: 154 LNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYKIN-SWEFG 213
           L      +   + F LNA +      G +    W+ S AE L +Y V K Y  N  WE G
Sbjct: 126 LVKLTKGSKMRLLFDLNAEVRTGYEIGKKMTSTWDSSEAEKLFKYCVSKGYGDNIDWELG 185

Query: 214 ICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAW 273
               +T            ++T  Q  ED   L +++++    +  K  +V P    D  W
Sbjct: 186 NEPDHTS---------AHNLTEKQVGEDFKALHKVLEKY--PTLNKGSLVGP----DVGW 245

Query: 274 YD-EFVRKTGPKAVDVLT----HHIYNMGAGHDPKLIYRFVNSTYLSQVSNTFEQLENVI 333
               +V+     A D +T    H  Y  G   D   +  ++++TY  ++   F+++++V+
Sbjct: 246 MGVSYVKGLADGAGDHVTAFTLHQYYFDGNTSD---VSTYLDATYFKKLQQLFDKVKDVL 305

Query: 334 QKNAPW--AAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNARLGMASLYNTKVYC 393
            KN+P      W+GE    Y  G+  +SD +++ F   +       +LG+++  N KV  
Sbjct: 306 -KNSPHKDKPLWLGETSSGYNSGTKDVSDRYVSGFLTLD-------KLGLSAANNVKVVI 365

Query: 394 RQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKID-NKVSSDLRSYAHCSRGRS 453
           RQT+  G+YG+L  +TL P PDY+   + + L+G  V K+D +  ++  R YA C++  S
Sbjct: 366 RQTIYNGYYGLLDKNTLEPNPDYWLMHVHNSLVGNTVFKVDVSDPTNKARVYAQCTKTNS 425

Query: 454 GVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQKRTALREEYHLTPKDGL 513
             T       + T F + + ++             D  LK  Q        Y LTP+ G 
Sbjct: 426 KHTQSRYYKGSLTIFALNVGDE-------------DVTLKIDQYSGKKIYSYILTPEGGQ 485

Query: 514 LRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAFVVLPDFVAPACK 553
           L S  VLLNG  L+   +  +P L     +S +   ++  +  F V+ D    ACK
Sbjct: 486 LTSQKVLLNGKELKLVSD-QLPELNA--DESKTSFTLSPKTFGFFVVSDANVEACK 488

BLAST of CmoCh06G002830 vs. ExPASy TrEMBL
Match: A0A6J1I2E3 (heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111470271 PE=3 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 8.3e-302
Identity = 514/552 (93.12%), Postives = 523/552 (94.75%), Query Frame = 0

Query: 1   MGYQILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD 60
           MGYQILLICVLAF+PTILGR TIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD
Sbjct: 1   MGYQILLICVLAFIPTILGRDTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD 60

Query: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC 120
           ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVG GFKGTC
Sbjct: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGTGFKGTC 120

Query: 121 HPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180
           HPFQA VGSLFDFS GCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN
Sbjct: 121 HPFQATVGSLFDFSAGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180

Query: 181 YSNAEALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREII 240
           YSNAEALIQYTVEKNY+INSWEF       GNEL GR+SIGASITAAQYAEDLLKLREII
Sbjct: 181 YSNAEALIQYTVEKNYRINSWEF-------GNELAGRLSIGASITAAQYAEDLLKLREII 240

Query: 241 DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFV 300
           DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPK VDVLTHHIYNMGAG+DPKLIYRFV
Sbjct: 241 DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKVVDVLTHHIYNMGAGNDPKLIYRFV 300

Query: 301 NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERK 360
           NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFIN F   +    
Sbjct: 301 NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINSFWYLD---- 360

Query: 361 KNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNK 420
              +LGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKI+NK
Sbjct: 361 ---QLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKINNK 420

Query: 421 VSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQK 480
           VSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLIT+QNDMNMSLRKSNTRTN SH K RQK
Sbjct: 421 VSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITVQNDMNMSLRKSNTRTNGSHSKYRQK 480

Query: 481 RTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAF 540
           RTA REEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAF
Sbjct: 481 RTASREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAF 538

Query: 541 VVLPDFVAPACK 553
           VV+PDFV PACK
Sbjct: 541 VVIPDFVTPACK 538

BLAST of CmoCh06G002830 vs. ExPASy TrEMBL
Match: A0A6J1FRW3 (heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111446673 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.3e-294
Identity = 501/552 (90.76%), Postives = 518/552 (93.84%), Query Frame = 0

Query: 1   MGYQILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD 60
           MGYQILLICVLAF+PTILGRGTI LGHDTTMGTIVIDGTK IAETDENFVCLTLDIWPHD
Sbjct: 1   MGYQILLICVLAFIPTILGRGTITLGHDTTMGTIVIDGTKMIAETDENFVCLTLDIWPHD 60

Query: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC 120
           ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKS+RIRVGGTLQDKLIYNVGAGFKGTC
Sbjct: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSLRIRVGGTLQDKLIYNVGAGFKGTC 120

Query: 121 HPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180
           HPFQAI GSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN
Sbjct: 121 HPFQAIEGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180

Query: 181 YSNAEALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREII 240
           YSNAEALIQYT EKNYKINSWEF       GNEL GRISIGASITAAQYAEDLLKLREII
Sbjct: 181 YSNAEALIQYTKEKNYKINSWEF-------GNELAGRISIGASITAAQYAEDLLKLREII 240

Query: 241 DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFV 300
           DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPK VDVLTHHIYNMGAGHDPKL+YRFV
Sbjct: 241 DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKVVDVLTHHIYNMGAGHDPKLVYRFV 300

Query: 301 NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERK 360
           NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFIN F   +    
Sbjct: 301 NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINSFWYLD---- 360

Query: 361 KNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNK 420
              +LGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGP VLKI N 
Sbjct: 361 ---QLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPRVLKIYNH 420

Query: 421 VSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQK 480
           VSSDLRSYAHCSRGRSGVTLLLINLSNTT+FLIT++++MNMSL+K NTRTNDSHLK R+K
Sbjct: 421 VSSDLRSYAHCSRGRSGVTLLLINLSNTTDFLITVESNMNMSLQKRNTRTNDSHLKHRRK 480

Query: 481 RTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAF 540
           RTA REEYHLTPKDGLLRSSTVLLN NPLETTKEGDIPNL+PVYRQSNS+I +ASWSIAF
Sbjct: 481 RTASREEYHLTPKDGLLRSSTVLLNENPLETTKEGDIPNLMPVYRQSNSFIRVASWSIAF 538

Query: 541 VVLPDFVAPACK 553
           +V+PDFVA  CK
Sbjct: 541 IVIPDFVAAGCK 538

BLAST of CmoCh06G002830 vs. ExPASy TrEMBL
Match: A0A6J1G385 (heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450381 PE=3 SV=1)

HSP 1 Score: 982.6 bits (2539), Expect = 6.6e-283
Identity = 487/522 (93.30%), Postives = 489/522 (93.68%), Query Frame = 0

Query: 31  MGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCVWDGHASMLNLDLTLPILNKAV 90
           MGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCVWDGHASMLNLDLTLPILNKAV
Sbjct: 1   MGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCVWDGHASMLNLDLTLPILNKAV 60

Query: 91  QAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGSLFDFSVGCLYMERWDDLNNFF 150
           QAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGSLFDFSVGCLYMERWDDLNNFF
Sbjct: 61  QAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGSLFDFSVGCLYMERWDDLNNFF 120

Query: 151 NNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYKINSWEFGICHHYT 210
           NNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYKINSWEF       
Sbjct: 121 NNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYKINSWEF------- 180

Query: 211 GNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYDEFVR 270
           GNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYDEFVR
Sbjct: 181 GNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAFFDEAWYDEFVR 240

Query: 271 KTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVSNTFEQLENVIQKNAPWAAAWV 330
           KTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVSNTFEQLENVIQKNAPWAAAWV
Sbjct: 241 KTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVSNTFEQLENVIQKNAPWAAAWV 300

Query: 331 GEAGGAYQGGSPHLSDTFINRFSPFEYERKKNARLGMASLYNTKVYCRQTLIGGFYGVLK 390
           GEAGGAYQGGSPHLSDTFIN F   +       +LGMASLYNTKVYCRQTLIGGFYGVLK
Sbjct: 301 GEAGGAYQGGSPHLSDTFINSFWYLD-------QLGMASLYNTKVYCRQTLIGGFYGVLK 360

Query: 391 SSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTN 450
           SSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTN
Sbjct: 361 SSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYAHCSRGRSGVTLLLINLSNTTN 420

Query: 451 FLITIQNDMNMSLRKSNTRTNDSHLKDRQKRTALREEYHLTPKDGLLRSSTVLLNGNPLE 510
           FLITIQNDMNMSL               QKRTALREEYHLTPKDGLLRSSTVLLNGNPLE
Sbjct: 421 FLITIQNDMNMSL---------------QKRTALREEYHLTPKDGLLRSSTVLLNGNPLE 480

Query: 511 TTKEGDIPNLVPVYRQSNSYIHIASWSIAFVVLPDFVAPACK 553
           TTKEGDIPNLVPVYRQSNSYIHIASWSIAFVVLPDFVAPACK
Sbjct: 481 TTKEGDIPNLVPVYRQSNSYIHIASWSIAFVVLPDFVAPACK 493

BLAST of CmoCh06G002830 vs. ExPASy TrEMBL
Match: A0A6J1HU12 (heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111466170 PE=3 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 1.1e-256
Identity = 443/551 (80.40%), Postives = 478/551 (86.75%), Query Frame = 0

Query: 1   MGYQILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD 60
           M  +I+LI VLAF+PTILGR       + T GTIV+D T TIAETDENFVC TLDIWPHD
Sbjct: 1   MENKIVLIFVLAFIPTILGR-------NATTGTIVVDATTTIAETDENFVCFTLDIWPHD 60

Query: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC 120
           ECRWSKLCVWDGHASMLNLDLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGF+GTC
Sbjct: 61  ECRWSKLCVWDGHASMLNLDLTLPIINKAVQAFKSVRIRVGGTLQDKLIYNVGAGFQGTC 120

Query: 121 HPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180
           HPFQA  GSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGK+ T+GIQWEG WN
Sbjct: 121 HPFQATKGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWN 180

Query: 181 YSNAEALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREII 240
           YSNAEALIQYTVE NY+INSWEF       GNEL G  SIGAS+TAAQYA+D++KLREII
Sbjct: 181 YSNAEALIQYTVENNYQINSWEF-------GNELAGINSIGASVTAAQYAKDVIKLREII 240

Query: 241 DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFV 300
           DRLYNNSQQKP+IVAPGAFFDE WY EF+RKTGP  VDVLTHHIYNMGAG DPKLIYRFV
Sbjct: 241 DRLYNNSQQKPMIVAPGAFFDEKWYAEFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFV 300

Query: 301 NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERK 360
           NSTYLSQVSNTFE+LE+VIQKNAPW AAWVGEAGGAYQGGSPH+S TFIN F   +    
Sbjct: 301 NSTYLSQVSNTFERLESVIQKNAPWGAAWVGEAGGAYQGGSPHISYTFINSFWYLD---- 360

Query: 361 KNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNK 420
              +LGMAS+YNTKVYCRQTLIGGFYGV+KSSTLLPTPDYYGALLFHRLMGP VLKIDN 
Sbjct: 361 ---QLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRVLKIDNN 420

Query: 421 VSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQK 480
           VSSDLRSYAHCSRGRSGVTLL INLSN T+F+I ++N++N+S  K N          R K
Sbjct: 421 VSSDLRSYAHCSRGRSGVTLLFINLSNATDFIIDLENNINLSSGKRNASKG-----QRVK 480

Query: 481 RTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAF 540
             + REEYHLTPKDGL+RSSTVLLNGNPLETTKEGDIPNL+PVYRQSNS IHIA WSIAF
Sbjct: 481 IDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLIPVYRQSNSSIHIAGWSIAF 525

Query: 541 VVLPDFVAPAC 552
           +V+P FVAPAC
Sbjct: 541 IVVPHFVAPAC 525

BLAST of CmoCh06G002830 vs. ExPASy TrEMBL
Match: A0A6J1EAY5 (heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111432361 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 4.0e-256
Identity = 441/551 (80.04%), Postives = 476/551 (86.39%), Query Frame = 0

Query: 1   MGYQILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD 60
           M  +I+LI VLAF+PTILGR       + T G+IV+D T TIAETDENFVC TLDIWPHD
Sbjct: 1   MENKIVLIFVLAFIPTILGR-------NATTGSIVVDVTTTIAETDENFVCFTLDIWPHD 60

Query: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC 120
           ECRWSKLCVWDGHASMLNLDLTLPI+NKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC
Sbjct: 61  ECRWSKLCVWDGHASMLNLDLTLPIINKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC 120

Query: 121 HPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180
           HPFQA  GSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGK+ T+GIQWEG WN
Sbjct: 121 HPFQATKGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKYKTQGIQWEGNWN 180

Query: 181 YSNAEALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREII 240
           YSN EALIQYT+E NY+INSWEF       GNEL G  SIGAS+TAAQYA+D++KLREII
Sbjct: 181 YSNTEALIQYTIENNYQINSWEF-------GNELAGLNSIGASVTAAQYAKDVIKLREII 240

Query: 241 DRLYNNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFV 300
           DRLYNN QQKP+IVAPGAFFDE WY EF+RKTGP  VDVLTHHIYNMGAG DPKLIYRFV
Sbjct: 241 DRLYNNFQQKPMIVAPGAFFDEKWYGEFLRKTGPGVVDVLTHHIYNMGAGDDPKLIYRFV 300

Query: 301 NSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERK 360
           NSTYLSQVSNTFE LENVIQKNAPW AAWVGEAGGAYQGGSPH+S TFIN F   +    
Sbjct: 301 NSTYLSQVSNTFEHLENVIQKNAPWGAAWVGEAGGAYQGGSPHISYTFINSFWYLD---- 360

Query: 361 KNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNK 420
              +LGMAS+YNTKVYCRQTLIGGFYGV+KSSTLLPTPDYYGALLFHRLMGP VLKIDN 
Sbjct: 361 ---QLGMASMYNTKVYCRQTLIGGFYGVIKSSTLLPTPDYYGALLFHRLMGPRVLKIDNN 420

Query: 421 VSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQK 480
           VSSDLRSYAHCSRGR+GVTLL INLSNTT+F+I ++N++N+S  K N          R K
Sbjct: 421 VSSDLRSYAHCSRGRTGVTLLFINLSNTTDFIIDVENNINLSSGKRNASKG-----QRMK 480

Query: 481 RTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAF 540
             + REEYHLTPKDGL+RSSTVLLNGNPLETTKEGDIPNL+PVYRQSNS IHIA WSIAF
Sbjct: 481 IDSPREEYHLTPKDGLVRSSTVLLNGNPLETTKEGDIPNLLPVYRQSNSSIHIAGWSIAF 525

Query: 541 VVLPDFVAPAC 552
           +V+P FVAPAC
Sbjct: 541 IVVPHFVAPAC 525

BLAST of CmoCh06G002830 vs. TAIR 10
Match: AT5G07830.1 (glucuronidase 2 )

HSP 1 Score: 550.1 bits (1416), Expect = 2.1e-156
Identity = 272/564 (48.23%), Postives = 385/564 (68.26%), Query Frame = 0

Query: 1   MGYQILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHD 60
           MG+++ +I V  F+  +L      +  +    +IVI G + + ETDENFVC TLD WPHD
Sbjct: 1   MGFRVCVIVV--FLGCLLLVPEKTMAQEMKRASIVIQGARRVCETDENFVCATLDWWPHD 60

Query: 61  ECRWSKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTC 120
           +C + + C W G++S++N+DLT P+L KA++AFK +RIR+GG+LQD++IY+VG   K  C
Sbjct: 61  KCNYDQ-CPW-GYSSVINMDLTRPLLTKAIKAFKPLRIRIGGSLQDQVIYDVG-NLKTPC 120

Query: 121 HPFQAIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWN 180
            PFQ +   LF FS GCL+M+RWD+LN+F   TGA+VTFGLNAL G+H  +G  W G W+
Sbjct: 121 RPFQKMNSGLFGFSKGCLHMKRWDELNSFLTATGAVVTFGLNALRGRHKLRGKAWGGAWD 180

Query: 181 YSNAEALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREII 240
           + N +  + YTV K Y I+SWEF       GNEL G   +GAS++A  Y +DL+ L+++I
Sbjct: 181 HINTQDFLNYTVSKGYVIDSWEF-------GNELSGS-GVGASVSAELYGKDLIVLKDVI 240

Query: 241 DRLYNNS-QQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRF 300
           +++Y NS   KP++VAPG F+++ WY + +  +GP  VDV+THHIYN+G+G+DP L+ + 
Sbjct: 241 NKVYKNSWLHKPILVAPGGFYEQQWYTKLLEISGPSVVDVVTHHIYNLGSGNDPALVKKI 300

Query: 301 VNSTYLSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYER 360
           ++ +YLSQVS TF+ +   IQ++ PWA+ WVGE+GGAY  G  H+SDTFI+ F   +   
Sbjct: 301 MDPSYLSQVSKTFKDVNQTIQEHGPWASPWVGESGGAYNSGGRHVSDTFIDSFWYLD--- 360

Query: 361 KKNARLGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDN 420
               +LGM++ +NTKVYCRQTL+GGFYG+L+  T +P PDYY ALL+HRLMG GVL +  
Sbjct: 361 ----QLGMSARHNTKVYCRQTLVGGFYGLLEKGTFVPNPDYYSALLWHRLMGKGVLAVQT 420

Query: 421 KVSSDLRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLRKSNTRTNDSHLKDRQ 480
                LR YAHCS+GR+GVTLLLINLSN ++F +++ N +N+ L   + +     L D  
Sbjct: 421 DGPPQLRVYAHCSKGRAGVTLLLINLSNQSDFTVSVSNGINVVLNAESRKKKS--LLDTL 480

Query: 481 KRT------------ALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQS 540
           KR               REEYHLTP++G+LRS T++LNG  L+ T  GDIP+L PV R  
Sbjct: 481 KRPFSWIGSKASDGYLNREEYHLTPENGVLRSKTMVLNGKSLKPTATGDIPSLEPVLRSV 540

Query: 541 NSYIHIASWSIAFVVLPDFVAPAC 552
           NS +++   S++F+VLP+F A AC
Sbjct: 541 NSPLNVLPLSMSFIVLPNFDASAC 542

BLAST of CmoCh06G002830 vs. TAIR 10
Match: AT5G61250.2 (glucuronidase 1 )

HSP 1 Score: 537.0 bits (1382), Expect = 1.8e-152
Identity = 271/552 (49.09%), Postives = 367/552 (66.49%), Query Frame = 0

Query: 10  VLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCV 69
           V+ F+  +L    +  G +    T+VIDG++ IAETDENF+C TLD WP ++C + + C 
Sbjct: 5   VVVFLSCLLLLPPVTFGSNMERTTLVIDGSRRIAETDENFICATLDWWPPEKCNYDQ-CP 64

Query: 70  WDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGS 129
           W G+AS++NL+L  P+L KA+QAF+++RIR+GG+LQD++IY+VG   K  C  F+     
Sbjct: 65  W-GYASLINLNLASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVG-DLKTPCTQFKKTDDG 124

Query: 130 LFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQ 189
           LF FS GCLYM+RWD++N+FFN TGAIVTFGLNAL G++   G  W G W+++N +  + 
Sbjct: 125 LFGFSEGCLYMKRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMN 184

Query: 190 YTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQ 249
           YTV K Y I+SWEF       GNEL G   I AS++   Y +DL+ L+ +I  +Y NS+ 
Sbjct: 185 YTVSKGYAIDSWEF-------GNELSGS-GIWASVSVELYGKDLIVLKNVIKNVYKNSRT 244

Query: 250 KPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVS 309
           KPL+VAPG FF+E WY E +R +GP  +DVLTHHIYN+G G+DPKL+ + ++  YLS +S
Sbjct: 245 KPLVVAPGGFFEEQWYSELLRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGIS 304

Query: 310 NTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNARLGMAS 369
             F  +   IQ++ PWAAAWVGEAGGA+  G   +S+TFIN F   +       +LG++S
Sbjct: 305 ELFANVNQTIQEHGPWAAAWVGEAGGAFNSGGRQVSETFINSFWYLD-------QLGISS 364

Query: 370 LYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYA 429
            +NTKVYCRQ L+GGFYG+L+  T +P PDYY ALL+HRLMG G+L +    S  LR+Y 
Sbjct: 365 KHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGILGVQTTASEYLRAYV 424

Query: 430 HCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSL------RKSNTRTNDSHLKDRQKRTA 489
           HCS+ R+G+T+LLINLS  T F + + N + + L      RKS   T  S +     + +
Sbjct: 425 HCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKAS 484

Query: 490 ----LREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIA 549
                REEYHL+PKDG LRS  +LLNG PL  T  GDIP L PV     S ++I   SI+
Sbjct: 485 DGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSIS 538

Query: 550 FVVLPDFVAPAC 552
           F+VLP F APAC
Sbjct: 545 FIVLPTFDAPAC 538

BLAST of CmoCh06G002830 vs. TAIR 10
Match: AT5G61250.1 (glucuronidase 1 )

HSP 1 Score: 537.0 bits (1382), Expect = 1.8e-152
Identity = 271/552 (49.09%), Postives = 367/552 (66.49%), Query Frame = 0

Query: 10  VLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHDECRWSKLCV 69
           V+ F+  +L    +  G +    T+VIDG++ IAETDENF+C TLD WP ++C + + C 
Sbjct: 5   VVVFLSCLLLLPPVTFGSNMERTTLVIDGSRRIAETDENFICATLDWWPPEKCNYDQ-CP 64

Query: 70  WDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQAIVGS 129
           W G+AS++NL+L  P+L KA+QAF+++RIR+GG+LQD++IY+VG   K  C  F+     
Sbjct: 65  W-GYASLINLNLASPLLAKAIQAFRTLRIRIGGSLQDQVIYDVG-DLKTPCTQFKKTDDG 124

Query: 130 LFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQ 189
           LF FS GCLYM+RWD++N+FFN TGAIVTFGLNAL G++   G  W G W+++N +  + 
Sbjct: 125 LFGFSEGCLYMKRWDEVNHFFNATGAIVTFGLNALHGRNKLNGTAWGGDWDHTNTQDFMN 184

Query: 190 YTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQ 249
           YTV K Y I+SWEF       GNEL G   I AS++   Y +DL+ L+ +I  +Y NS+ 
Sbjct: 185 YTVSKGYAIDSWEF-------GNELSGS-GIWASVSVELYGKDLIVLKNVIKNVYKNSRT 244

Query: 250 KPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVS 309
           KPL+VAPG FF+E WY E +R +GP  +DVLTHHIYN+G G+DPKL+ + ++  YLS +S
Sbjct: 245 KPLVVAPGGFFEEQWYSELLRLSGPGVLDVLTHHIYNLGPGNDPKLVNKILDPNYLSGIS 304

Query: 310 NTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNARLGMAS 369
             F  +   IQ++ PWAAAWVGEAGGA+  G   +S+TFIN F   +       +LG++S
Sbjct: 305 ELFANVNQTIQEHGPWAAAWVGEAGGAFNSGGRQVSETFINSFWYLD-------QLGISS 364

Query: 370 LYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYA 429
            +NTKVYCRQ L+GGFYG+L+  T +P PDYY ALL+HRLMG G+L +    S  LR+Y 
Sbjct: 365 KHNTKVYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKGILGVQTTASEYLRAYV 424

Query: 430 HCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSL------RKSNTRTNDSHLKDRQKRTA 489
           HCS+ R+G+T+LLINLS  T F + + N + + L      RKS   T  S +     + +
Sbjct: 425 HCSKRRAGITILLINLSKHTTFTVAVSNGVKVVLQAESMKRKSFLETIKSKVSWVGNKAS 484

Query: 490 ----LREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIA 549
                REEYHL+PKDG LRS  +LLNG PL  T  GDIP L PV     S ++I   SI+
Sbjct: 485 DGYLNREEYHLSPKDGDLRSKIMLLNGKPLVPTATGDIPKLEPVRHGVKSPVYINPLSIS 538

Query: 550 FVVLPDFVAPAC 552
           F+VLP F APAC
Sbjct: 545 FIVLPTFDAPAC 538

BLAST of CmoCh06G002830 vs. TAIR 10
Match: AT5G34940.2 (glucuronidase 3 )

HSP 1 Score: 424.9 bits (1091), Expect = 1.0e-118
Identity = 232/552 (42.03%), Postives = 323/552 (58.51%), Query Frame = 0

Query: 5   ILLICVLAFVPTILGRGTIILGHDTTMGTIVIDGTKTIAETDENFVCLTLDIWPHDECRW 64
           +L +CV  F+   +             GT+ + G   +   DE+F+C TLD WP ++C +
Sbjct: 10  VLFLCVFQFLDCTVSSAV------EENGTVFVYGRAAVGTIDEDFICATLDWWPPEKCDY 69

Query: 65  SKLCVWDGHASMLNLDLTLPILNKAVQAFKSVRIRVGGTLQDKLIYNVGAGFKGTCHPFQ 124
              C WD HAS+LNLDL   IL  A++AF  ++IR+GGTLQD +IY      K  C PF 
Sbjct: 70  GS-CSWD-HASILNLDLNNVILQNAIKAFAPLKIRIGGTLQDIVIYET-PDSKQPCLPFT 129

Query: 125 AIVGSLFDFSVGCLYMERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNA 184
                LF ++ GCL M RWD+LN FF  TG  V FGLNAL G+      +  G WNY+NA
Sbjct: 130 KNSSILFGYTQGCLPMRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNA 189

Query: 185 EALIQYTVEKNYKINSWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLY 244
           E+ I++T E NY I+ WE        GNEL G   +GA + A QYA D + LR I++R+Y
Sbjct: 190 ESFIRFTAENNYTIDGWEL-------GNELCGS-GVGARVGANQYAIDTINLRNIVNRVY 249

Query: 245 NNSQQKPLIVAPGAFFDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTY 304
            N    PL++ PG FF+  W+ E++ K    +++  T HIY++G G D  LI + +N +Y
Sbjct: 250 KNVSPMPLVIGPGGFFEVDWFTEYLNK-AENSLNATTRHIYDLGPGVDEHLIEKILNPSY 309

Query: 305 LSQVSNTFEQLENVIQKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNAR 364
           L Q + +F  L+N+I+ ++  A AWVGE+GGAY  G   +S+ F+  F   +       +
Sbjct: 310 LDQEAKSFRSLKNIIKNSSTKAVAWVGESGGAYNSGRNLVSNAFVYSFWYLD-------Q 369

Query: 365 LGMASLYNTKVYCRQTLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSD 424
           LGMASLY+TK YCRQ+LIGG YG+L ++   P PDYY AL++ +LMG   L      +  
Sbjct: 370 LGMASLYDTKTYCRQSLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGRKALFTTFSGTKK 429

Query: 425 LRSYAHCSRGRSGVTLLLINLSNTTNFLITIQNDMNMSLR-----KSNTRTNDSHLKDRQ 484
           +RSY HC+R   G+T+LL+NL NTT  +  ++ + + SLR     KS  R + S L    
Sbjct: 430 IRSYTHCARQSKGITVLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSYKRAS-SQLFGGP 489

Query: 485 KRTALREEYHLTPKDGLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIA 544
                REEYHLT KDG L S T+LLNGN L+    GD+P + P++  S   I IA +SI 
Sbjct: 490 NGVIQREEYHLTAKDGNLHSQTMLLNGNALQVNSMGDLPPIEPIHINSTEPITIAPYSIV 535

Query: 545 FVVLPDFVAPAC 552
           FV + + V PAC
Sbjct: 550 FVHMRNVVVPAC 535

BLAST of CmoCh06G002830 vs. TAIR 10
Match: AT5G34940.1 (glucuronidase 3 )

HSP 1 Score: 336.7 bits (862), Expect = 3.6e-92
Identity = 180/417 (43.17%), Postives = 250/417 (59.95%), Query Frame = 0

Query: 140 MERWDDLNNFFNNTGAIVTFGLNALLGKHNTKGIQWEGKWNYSNAEALIQYTVEKNYKIN 199
           M RWD+LN FF  TG  V FGLNAL G+      +  G WNY+NAE+ I++T E NY I+
Sbjct: 1   MRRWDELNAFFRKTGTKVIFGLNALSGRSIKSNGEAIGAWNYTNAESFIRFTAENNYTID 60

Query: 200 SWEFGICHHYTGNELVGRISIGASITAAQYAEDLLKLREIIDRLYNNSQQKPLIVAPGAF 259
            WE        GNEL G   +GA + A QYA D + LR I++R+Y N    PL++ PG F
Sbjct: 61  GWEL-------GNELCGS-GVGARVGANQYAIDTINLRNIVNRVYKNVSPMPLVIGPGGF 120

Query: 260 FDEAWYDEFVRKTGPKAVDVLTHHIYNMGAGHDPKLIYRFVNSTYLSQVSNTFEQLENVI 319
           F+  W+ E++ K    +++  T HIY++G G D  LI + +N +YL Q + +F  L+N+I
Sbjct: 121 FEVDWFTEYLNK-AENSLNATTRHIYDLGPGVDEHLIEKILNPSYLDQEAKSFRSLKNII 180

Query: 320 QKNAPWAAAWVGEAGGAYQGGSPHLSDTFINRFSPFEYERKKNARLGMASLYNTKVYCRQ 379
           + ++  A AWVGE+GGAY  G   +S+ F+  F   +       +LGMASLY+TK YCRQ
Sbjct: 181 KNSSTKAVAWVGESGGAYNSGRNLVSNAFVYSFWYLD-------QLGMASLYDTKTYCRQ 240

Query: 380 TLIGGFYGVLKSSTLLPTPDYYGALLFHRLMGPGVLKIDNKVSSDLRSYAHCSRGRSGVT 439
           +LIGG YG+L ++   P PDYY AL++ +LMG   L      +  +RSY HC+R   G+T
Sbjct: 241 SLIGGNYGLLNTTNFTPNPDYYSALIWRQLMGRKALFTTFSGTKKIRSYTHCARQSKGIT 300

Query: 440 LLLINLSNTTNFLITIQNDMNMSLR-----KSNTRTNDSHLKDRQKRTALREEYHLTPKD 499
           +LL+NL NTT  +  ++ + + SLR     KS  R + S L         REEYHLT KD
Sbjct: 301 VLLMNLDNTTTVVAKVELNNSFSLRHTKHMKSYKRAS-SQLFGGPNGVIQREEYHLTAKD 360

Query: 500 GLLRSSTVLLNGNPLETTKEGDIPNLVPVYRQSNSYIHIASWSIAFVVLPDFVAPAC 552
           G L S T+LLNGN L+    GD+P + P++  S   I IA +SI FV + + V PAC
Sbjct: 361 GNLHSQTMLLNGNALQVNSMGDLPPIEPIHINSTEPITIAPYSIVFVHMRNVVVPAC 400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FF102.9e-15548.23Heparanase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g07830 PE=2 SV=1[more]
Q8L6082.6e-15149.09Heparanase-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At5g61250 PE=2 SV=1[more]
Q9FZP11.4e-11742.03Heparanase-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g34940 PE=2 SV=2[more]
Q9LRC89.0e-9637.43Baicalin-beta-D-glucuronidase OS=Scutellaria baicalensis OX=65409 GN=SGUS PE=1 S... [more]
X4Y2L41.6e-3125.56Hyaluronoglucuronidase OS=Hirudo nipponia OX=42736 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I2E38.3e-30293.12heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111470271 PE=3 SV=1[more]
A0A6J1FRW31.3e-29490.76heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111446673 PE=3 SV=... [more]
A0A6J1G3856.6e-28393.30heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450381 PE=3 SV=... [more]
A0A6J1HU121.1e-25680.40heparanase-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111466170 PE=3 SV=1[more]
A0A6J1EAY54.0e-25680.04heparanase-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111432361 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G07830.12.1e-15648.23glucuronidase 2 [more]
AT5G61250.21.8e-15249.09glucuronidase 1 [more]
AT5G61250.11.8e-15249.09glucuronidase 1 [more]
AT5G34940.21.0e-11842.03glucuronidase 3 [more]
AT5G34940.13.6e-9243.17glucuronidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 226..246
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 71..408
e-value: 1.5E-51
score: 177.6
NoneNo IPR availablePANTHERPTHR14363HEPARANASE-RELATEDcoord: 13..552
NoneNo IPR availablePANTHERPTHR14363:SF21HEPARANASE-LIKE PROTEIN 1coord: 13..552
IPR005199Glycoside hydrolase, family 79PFAMPF03662Glyco_hydro_79ncoord: 32..352
e-value: 5.2E-124
score: 413.3
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 45..410

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh06G002830.1CmoCh06G002830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0004566 beta-glucuronidase activity
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds