CmoCh06G002820 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G002820
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	tropinone reductase homolog
Location	Cmo_Chr06: 1411889 .. 1413917 (-)
RNA-Seq Expression	CmoCh06G002820
Synteny	CmoCh06G002820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAACACCGACAGAGATCAAAGATGGTCGCTTCAGGGGATGACTGCTCTAGTCACCGGCGGAACCCACGGCATTGGGTACTCATAATTTCCAGCTCCTCCTTTCCCTTTTGGTGTCTCTGTTCTAACCCATTTATTTCAGGCGAGCCACGGTGGAGGAGCTTGCAGCATTTGGTGCCACTGTCCACACATGCTCGCGAAGCCAGGGGGATTTGGACAAATGCCTGAAAGAATGGGAAGAAATGGGGTTCAATGTAAGTGGGTCTGTGTGCGATGTGCATAGCAGAGAACAGAGGATGCAGCTGATGGAAACCGTGTCGTCTCTGTTCAATGGAAGCCTCAACATTTTGGTAACGAAAATGAGAAAAATGGTGTGTTCATAATGGATGTGTAAGTGTTTTAGTATGAAGCTCTGTTTTTGAGGTGTTTCAGGTGAATAATGCTGGGACATGGGCGTACAAGAACTCCGAAGACGTTACGGAGGAAGAGCTTTCGAGTGTTATGAGTACCAATTTTGAAGCGTCGTTTCATTTTAGCCAACTCGCTCATCCTTTGATGAAAGCTTCTGGTAATGGAAGCATTGTGTTCATGTCCTCTGTTGCAGGCTCGATGTCGCTTCCACTATCTACTGCATACGCTGCATCCAAAGGTAACCCACAATTTTATTCACCGATAAGTAGCACACAGCTAGTAGATATTATTTTTTTTTAGATTTTCTCACTCGAGCTTTCCTTTTAACGGTTTCAAAACGCTTTTACACCCTTACAAAAAATGCTTCGTTCTCTCCTCTAACCGACATGGGATCTCACAATCCACCACTCTTGGGGTCCAGCGTTATTGTAGGCACACCGTGTTGAGGATTATTGGGAGTGAGTCCCACATTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGAGGAATACTCTTGGGGTCGAGCGTTATTGTGGGCACACCGCTTATTGTCTAGCTCTGATATCATTTATAACCACTCAAACCCACTTCTAGCATATATTATCAAAAGGGAAAGCGAGTGTTATTGTGGGCACACCGCTTATTGTCTAGCTCTGATATCATTTATAACCACTCAAACCCACTTCTAGCATATATTATCAAAAGGGAAAGCGAGCGTTATTGTGGGCACACCGCTTATTGTCTAGCTCTGATATCATTTATAACCACACAAAACCCACTTCTAGCATATATTATCAAAAGGGAAAGCCTGGAAAATAAATGCTAGCAATGGACTTAGTATGTTACAAAATGATATCAGAGTCAAGTTTCAAATGGTATGTCGGTGAAAGAATGAGTGATTGAATGGTTGATAAGAAGATTATATAATAAAAAGCGAGAACGCTCTATATATATAGAAAGAATACAAAAAAATATTTAGAAACTGACAGCAATCTAAACAAACACGTGATCTTTAACACGGATGCTGGCGGATATTTAATCAGATACTAACTCTAAACAAACACGTGATCTTTAACAAGGTTACTGGCCTCCAAGAGGGTGAATGGGAACGAAACATGATATGTAACTGGCCAAAACGGACAATATTTACTTGCGGTAGGGTTGGACGGTTACAAATTTAAATTAATCTTCAATATTTAGAGTATGTTCTTACAATGGATTTGTTGATGAAGCTGCCATAAACCAAATCACAAGGAACTTAGCATGCGAATGGGCCAAAGACAACATCCGCACAAACGCAGTGGCACCATGGATCATCAAAACTCGCTTTGTGAAACCTCCCACCGTAAGAATTTCAATTCAAATCCATCCTGGTTGGGTTCTACAAACTTATAGCTGAATCTTTGTCCCATTTTCATCAGGATGATCCGATCCATGTAGAAGTAATGAACCACATGCTGTCAGTAACACCTCTCAAACGTGCTGGGGAGCCACATGAAGTTTCTTCAATGGTTGCCTTTCTGTGTCTTCCAACAGCGTCTTATATTACTGGACAAGTTTTGTATGTTGATGGTGGCCACACCGTGAACGCTTTCCCCATCCCCAATGCTTGA

mRNA sequence

ATGGCGAACACCGACAGAGATCAAAGATGGTCGCTTCAGGGGATGACTGCTCTAGTCACCGGCGGAACCCACGGCATTGGGCGAGCCACGGTGGAGGAGCTTGCAGCATTTGGTGCCACTGTCCACACATGCTCGCGAAGCCAGGGGGATTTGGACAAATGCCTGAAAGAATGGGAAGAAATGGGGTTCAATGTAAGTGGGTCTGTGTGCGATGTGCATAGCAGAGAACAGAGGATGCAGCTGATGGAAACCGTGTCGTCTCTGTTCAATGGAAGCCTCAACATTTTGGTAACGAAAATGAGAAAAATGGTGAATAATGCTGGGACATGGGCGTACAAGAACTCCGAAGACGTTACGGAGGAAGAGCTTTCGAGTGTTATGAGTACCAATTTTGAAGCGTCGTTTCATTTTAGCCAACTCGCTCATCCTTTGATGAAAGCTTCTGGTAATGGAAGCATTGTGTTCATGTCCTCTGTTGCAGGCTCGATGAACTTAGCATGCGAATGGGCCAAAGACAACATCCGCACAAACGCAGTGGCACCATGGATCATCAAAACTCGCTTTGTGAAACCTCCCACCGATGATCCGATCCATGTAGAAGTAATGAACCACATGCTGTCAGTAACACCTCTCAAACGTGCTGGGGAGCCACATGAAGTTTCTTCAATGGTTGCCTTTCTGTGTCTTCCAACAGCGTCTTATATTACTGGACAAGTTTTGTATGTTGATGGTGGCCACACCGTGAACGCTTTCCCCATCCCCAATGCTTGA

Coding sequence (CDS)

ATGGCGAACACCGACAGAGATCAAAGATGGTCGCTTCAGGGGATGACTGCTCTAGTCACCGGCGGAACCCACGGCATTGGGCGAGCCACGGTGGAGGAGCTTGCAGCATTTGGTGCCACTGTCCACACATGCTCGCGAAGCCAGGGGGATTTGGACAAATGCCTGAAAGAATGGGAAGAAATGGGGTTCAATGTAAGTGGGTCTGTGTGCGATGTGCATAGCAGAGAACAGAGGATGCAGCTGATGGAAACCGTGTCGTCTCTGTTCAATGGAAGCCTCAACATTTTGGTAACGAAAATGAGAAAAATGGTGAATAATGCTGGGACATGGGCGTACAAGAACTCCGAAGACGTTACGGAGGAAGAGCTTTCGAGTGTTATGAGTACCAATTTTGAAGCGTCGTTTCATTTTAGCCAACTCGCTCATCCTTTGATGAAAGCTTCTGGTAATGGAAGCATTGTGTTCATGTCCTCTGTTGCAGGCTCGATGAACTTAGCATGCGAATGGGCCAAAGACAACATCCGCACAAACGCAGTGGCACCATGGATCATCAAAACTCGCTTTGTGAAACCTCCCACCGATGATCCGATCCATGTAGAAGTAATGAACCACATGCTGTCAGTAACACCTCTCAAACGTGCTGGGGAGCCACATGAAGTTTCTTCAATGGTTGCCTTTCTGTGTCTTCCAACAGCGTCTTATATTACTGGACAAGTTTTGTATGTTGATGGTGGCCACACCGTGAACGCTTTCCCCATCCCCAATGCTTGA

Protein sequence

MANTDRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVSGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSMNLACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLCLPTASYITGQVLYVDGGHTVNAFPIPNA

Homology

[Close]

BLAST of CmoCh06G002820 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 9.7e-77
Identity = 151/265 (56.98%), Postives = 182/265 (68.68%), Query Frame = 0


Query: 7   DQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVS 66
           D+RWSL+GMTALVTGGT GIG A VEELA FGA V+TCSRSQ DLD+CL++W   GF VS
Sbjct: 10  DRRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVS 69

Query: 67  GSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSV 126
           G VCDV S  QR  LME+V+S FNG LNIL       +NNAGT   K + + T E+ S +
Sbjct: 70  GPVCDVSSISQRQTLMESVTSSFNGKLNIL-------INNAGTTIPKEATNFTAEDYSII 129

Query: 127 MSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLA 186
           M TNFEAS++  QLAHPL+KASGN SIVF SS AG                    + +LA
Sbjct: 130 MGTNFEASYNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLA 189

Query: 187 CEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAF 246
           CEWAKD+IR NAVAPWII T  ++     P   + +  ++   P+KRAGEP EVSS+V +
Sbjct: 190 CEWAKDSIRVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTY 249

Query: 247 LCLPTASYITGQVLYVDGGHTVNAF 252
           LCLPTASYITGQ++ VDGG+TVN F
Sbjct: 250 LCLPTASYITGQIICVDGGYTVNGF 267

[Close]

BLAST of CmoCh06G002820 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.9e-73
Identity = 149/267 (55.81%), Postives = 180/267 (67.42%), Query Frame = 0


Query: 5   DRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFN 64
           + D+RWSL G TALVTGGT GIGRA VEELA FGA VHTCSR+Q +L+ CL +W+  G  
Sbjct: 2   ETDKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLV 61

Query: 65  VSGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELS 124
           VSGSVCD   R+QR +L++  SS F+G LNIL       +NN GT   K + + + EE +
Sbjct: 62  VSGSVCDASVRDQREKLIQEASSAFSGKLNIL-------INNVGTNVRKPTVEYSSEEYA 121

Query: 125 SVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMN 184
            +MSTN E++FH SQ+AHPL+KASG GSIVF+SSVAG                    + N
Sbjct: 122 KIMSTNLESAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRN 181

Query: 185 LACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMV 244
           LACEWA DNIRTN VAPW IKT  V+   +    VE    ++S TPL R GEP EVSS+V
Sbjct: 182 LACEWASDNIRTNCVAPWYIKTSLVETLLEKKEFVEA---VVSRTPLGRVGEPEEVSSLV 241

Query: 245 AFLCLPTASYITGQVLYVDGGHTVNAF 252
           AFLCLP +SYITGQV+ VDGG TVN F
Sbjct: 242 AFLCLPASSYITGQVISVDGGFTVNGF 258

[Close]

BLAST of CmoCh06G002820 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 5.5e-72
Identity = 149/266 (56.02%), Postives = 177/266 (66.54%), Query Frame = 0


Query: 7   DQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVS 66
           D+RWSLQGM ALVTGGT GIG A VEEL+  GA VHTC+R +  L + L+EW+E GF V+
Sbjct: 2   DKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVT 61

Query: 67  GSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSV 126
            S+CDV  REQR +LMETVSSLF G LNIL       VNN GT   K + + T EE S +
Sbjct: 62  TSICDVSLREQREKLMETVSSLFQGKLNIL-------VNNVGTLMLKPTTEYTAEEFSFL 121

Query: 127 MSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLA 186
           M+TN +++FH SQLAHPL+KASG+GSIV MSS+AG                    + NLA
Sbjct: 122 MATNLDSAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLA 181

Query: 187 CEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSV-TPLKRAGEPHEVSSMVA 246
           CEWA DNIRTNA+ PW+I T    P   D + VE M       TP+ R GE +EVS +VA
Sbjct: 182 CEWASDNIRTNAICPWLITT----PLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVA 241

Query: 247 FLCLPTASYITGQVLYVDGGHTVNAF 252
           FLCLP ASYITGQV+ VDGG TVN F
Sbjct: 242 FLCLPAASYITGQVICVDGGLTVNGF 256

[Close]

BLAST of CmoCh06G002820 vs. ExPASy Swiss-Prot
Match: Q9ZW20 (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 7.9e-71
Identity = 143/266 (53.76%), Postives = 177/266 (66.54%), Query Frame = 0


Query: 9   RWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVSGS 68
           +WSL+GMTALVTGG+ G+G+A VEELA  GA VHTC+R +  L + L+EW+  G  V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 69  VCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSVMS 128
           VCDV SR+QR +LMETVSSLF G L+ILV        N G    K + + T EE S +++
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVP-------NVGIGVLKPTTECTAEEFSFIIA 132

Query: 129 TNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLACE 188
           TN E++FHFSQLAHPL+KASG+G+IV MSSVAG                    + NLACE
Sbjct: 133 TNLESTFHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACE 192

Query: 189 WAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLC 248
           WA DNIR N+V PW I T   K    D    +V   + SVTPL+R GE +EVSS+VAFLC
Sbjct: 193 WASDNIRANSVCPWFITTPSTKDFLGDK---DVKEKVESVTPLRRVGEANEVSSLVAFLC 252

Query: 249 LPTASYITGQVLYVDGGHTVNAFPIP 255
           LP ASYITGQ + VDGG T+N F +P
Sbjct: 253 LPAASYITGQTICVDGGFTINGFSLP 268

[Close]

BLAST of CmoCh06G002820 vs. ExPASy Swiss-Prot
Match: Q9ZW18 (Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.8e-70
Identity = 144/265 (54.34%), Postives = 176/265 (66.42%), Query Frame = 0


Query: 7   DQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVS 66
           + RWSL GMTALVTGG+ GIG A VEELA  GA VHTC+R +  L + L+EW+  GF V+
Sbjct: 10  NSRWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLREWQAKGFQVT 69

Query: 67  GSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSV 126
            SVCDV SR+QR++LMETVSSL+ G LNIL       VNN GT  +K + + T E+ S V
Sbjct: 70  TSVCDVSSRDQRVKLMETVSSLYQGKLNIL-------VNNVGTSIFKPTTEYTAEDFSFV 129

Query: 127 MSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLA 186
           M+TN E++FH SQLAHPL+KASG+GSIV +SS AG                    + NLA
Sbjct: 130 MATNLESAFHLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATKGAMNQLARNLA 189

Query: 187 CEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAF 246
           CEWA DNIRTN+V PW I T    P ++D    E     +  TP+ R GE +EVS +VAF
Sbjct: 190 CEWASDNIRTNSVCPWYITT----PLSNDFFDEEFKKEAVRTTPMGRVGEANEVSPLVAF 249

Query: 247 LCLPTASYITGQVLYVDGGHTVNAF 252
           LCLP+ASYITGQ + VDGG TVN F
Sbjct: 250 LCLPSASYITGQTICVDGGATVNGF 263

[Close]

BLAST of CmoCh06G002820 vs. ExPASy TrEMBL
Match: A0A6J1FN65 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446688 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.8e-134
Identity = 249/276 (90.22%), Postives = 249/276 (90.22%), Query Frame = 0


Query: 1   MANTDRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60
           MANTDRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE
Sbjct: 1   MANTDRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFNVSGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTE 120
           MGFNVSGSVCDVHSREQRMQLMETVSSLFNGSLNIL       VNNAGTWAYKNSEDVTE
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLMETVSSLFNGSLNIL-------VNNAGTWAYKNSEDVTE 120

Query: 121 EELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSM----------------- 180
           EELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSM                 
Sbjct: 121 EELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSMSLPLSTAYAASKAAINQ 180

Query: 181 ---NLACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV 240
              NLACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV
Sbjct: 181 ITRNLACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV 240

Query: 241 SSMVAFLCLPTASYITGQVLYVDGGHTVNAFPIPNA 257
           SSMVAFLCLPTASYITGQVLYVDGGHTVNAFPIPNA
Sbjct: 241 SSMVAFLCLPTASYITGQVLYVDGGHTVNAFPIPNA 269

[Close]

BLAST of CmoCh06G002820 vs. ExPASy TrEMBL
Match: A0A6J1FKX7 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 9.9e-125
Identity = 233/276 (84.42%), Postives = 241/276 (87.32%), Query Frame = 0


Query: 1   MANTDRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60
           MANT RDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFNVSGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTE 120
           MGFNVSGSVCDVHSREQRMQL+ETVSSLFNGSLNIL       VNNAGT AYK+S +VTE
Sbjct: 61  MGFNVSGSVCDVHSREQRMQLIETVSSLFNGSLNIL-------VNNAGTSAYKSSLEVTE 120

Query: 121 EELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSM----------------- 180
           EELSSVMSTNFEA+FHFSQLAHPLMKASGNGSIVFMSSVAG M                 
Sbjct: 121 EELSSVMSTNFEAAFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQ 180

Query: 181 ---NLACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV 240
              NLACEWAKDNIRTNAVAPWII+TRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV
Sbjct: 181 ITRNLACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV 240

Query: 241 SSMVAFLCLPTASYITGQVLYVDGGHTVNAFPIPNA 257
           SSMVAFLCLP ASYITGQV  +DGGHTV AFP+P+A
Sbjct: 241 SSMVAFLCLPAASYITGQVFCIDGGHTVKAFPVPDA 269

[Close]

BLAST of CmoCh06G002820 vs. ExPASy TrEMBL
Match: A0A6J1I667 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.2e-123
Identity = 232/276 (84.06%), Postives = 238/276 (86.23%), Query Frame = 0


Query: 1   MANTDRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEE 60
           MANT RDQRWSLQGMTALVTGGTHGIGRATVEELAAFGA VHTCSRSQGDLDKCLKEWEE
Sbjct: 1   MANTSRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGAIVHTCSRSQGDLDKCLKEWEE 60

Query: 61  MGFNVSGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTE 120
           MG NVSGSVCDVHSREQR +LMETVSSLFNGSLNIL       VNNAGT AYK+S +VTE
Sbjct: 61  MGLNVSGSVCDVHSREQRKRLMETVSSLFNGSLNIL-------VNNAGTSAYKSSLEVTE 120

Query: 121 EELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSM----------------- 180
           EELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG M                 
Sbjct: 121 EELSSVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGLMSLPYSTPYAASKAAINQ 180

Query: 181 ---NLACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV 240
              NLACEWAKDNIRTNAVAPWII+TRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV
Sbjct: 181 ITRNLACEWAKDNIRTNAVAPWIIRTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEV 240

Query: 241 SSMVAFLCLPTASYITGQVLYVDGGHTVNAFPIPNA 257
           SSMVAFLCLP ASYITGQV  +DGGHTV AFPIP+A
Sbjct: 241 SSMVAFLCLPAASYITGQVFCIDGGHTVTAFPIPDA 269

[Close]

BLAST of CmoCh06G002820 vs. ExPASy TrEMBL
Match: A0A6J1CX69 (tropinone reductase homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015115 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.5e-104
Identity = 197/270 (72.96%), Postives = 218/270 (80.74%), Query Frame = 0


Query: 6   RDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNV 65
           R QRWSL+GMTALVTGGT GIGRATVEELAAFGA VHTCSRSQ DLD+CLKEW+EMGF V
Sbjct: 9   RGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEV 68

Query: 66  SGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSS 125
           +GSVCDVH REQR +LMETVSSLFN +LNIL       VNNAGT   K++ ++TE ELS 
Sbjct: 69  TGSVCDVHIREQREKLMETVSSLFNATLNIL-------VNNAGTMVIKSAAEITEAELSR 128

Query: 126 VMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSM--------------------NL 185
           VMSTNFEASFHF QLAHPL+KASG GSIVF+SSV+G M                    NL
Sbjct: 129 VMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGAINQITKNL 188

Query: 186 ACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVA 245
           ACEWAKDNIR NAVAPWIIKTRFV+PP+D+P+HVE M +MLSVTPLKR GEPHEVSS+VA
Sbjct: 189 ACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSVTPLKRGGEPHEVSSLVA 248

Query: 246 FLCLPTASYITGQVLYVDGGHTVNAFPIPN 256
           FLCLP ASYITGQV+ VDGGHTV AFP P+
Sbjct: 249 FLCLPAASYITGQVICVDGGHTVKAFPFPD 271

[Close]

BLAST of CmoCh06G002820 vs. ExPASy TrEMBL
Match: A0A6J1CVA8 (tropinone reductase homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015115 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.4e-102
Identity = 197/287 (68.64%), Postives = 218/287 (75.96%), Query Frame = 0


Query: 6   RDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNV 65
           R QRWSL+GMTALVTGGT GIGRATVEELAAFGA VHTCSRSQ DLD+CLKEW+EMGF V
Sbjct: 9   RGQRWSLKGMTALVTGGTRGIGRATVEELAAFGAVVHTCSRSQQDLDQCLKEWQEMGFEV 68

Query: 66  SGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSS 125
           +GSVCDVH REQR +LMETVSSLFN +LNIL       VNNAGT   K++ ++TE ELS 
Sbjct: 69  TGSVCDVHIREQREKLMETVSSLFNATLNIL-------VNNAGTMVIKSAAEITEAELSR 128

Query: 126 VMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAGSM---------------------- 185
           VMSTNFEASFHF QLAHPL+KASG GSIVF+SSV+G M                      
Sbjct: 129 VMSTNFEASFHFCQLAHPLLKASGYGSIVFISSVSGLMSLPLSTAYAASKGFFVTKGVSH 188

Query: 186 ---------------NLACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSV 245
                          NLACEWAKDNIR NAVAPWIIKTRFV+PP+D+P+HVE M +MLSV
Sbjct: 189 KGLFVKEGAINQITKNLACEWAKDNIRANAVAPWIIKTRFVEPPSDNPVHVETMKYMLSV 248

Query: 246 TPLKRAGEPHEVSSMVAFLCLPTASYITGQVLYVDGGHTVNAFPIPN 256
           TPLKR GEPHEVSS+VAFLCLP ASYITGQV+ VDGGHTV AFP P+
Sbjct: 249 TPLKRGGEPHEVSSLVAFLCLPAASYITGQVICVDGGHTVKAFPFPD 288

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BLAST of CmoCh06G002820 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.1e-74
Identity = 149/267 (55.81%), Postives = 180/267 (67.42%), Query Frame = 0


Query: 5   DRDQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFN 64
           + D+RWSL G TALVTGGT GIGRA VEELA FGA VHTCSR+Q +L+ CL +W+  G  
Sbjct: 2   ETDKRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLV 61

Query: 65  VSGSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELS 124
           VSGSVCD   R+QR +L++  SS F+G LNIL       +NN GT   K + + + EE +
Sbjct: 62  VSGSVCDASVRDQREKLIQEASSAFSGKLNIL-------INNVGTNVRKPTVEYSSEEYA 121

Query: 125 SVMSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMN 184
            +MSTN E++FH SQ+AHPL+KASG GSIVF+SSVAG                    + N
Sbjct: 122 KIMSTNLESAFHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRN 181

Query: 185 LACEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMV 244
           LACEWA DNIRTN VAPW IKT  V+   +    VE    ++S TPL R GEP EVSS+V
Sbjct: 182 LACEWASDNIRTNCVAPWYIKTSLVETLLEKKEFVEA---VVSRTPLGRVGEPEEVSSLV 241

Query: 245 AFLCLPTASYITGQVLYVDGGHTVNAF 252
           AFLCLP +SYITGQV+ VDGG TVN F
Sbjct: 242 AFLCLPASSYITGQVISVDGGFTVNGF 258

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BLAST of CmoCh06G002820 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 3.9e-73
Identity = 149/266 (56.02%), Postives = 177/266 (66.54%), Query Frame = 0


Query: 7   DQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVS 66
           D+RWSLQGM ALVTGGT GIG A VEEL+  GA VHTC+R +  L + L+EW+E GF V+
Sbjct: 2   DKRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVT 61

Query: 67  GSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSV 126
            S+CDV  REQR +LMETVSSLF G LNIL       VNN GT   K + + T EE S +
Sbjct: 62  TSICDVSLREQREKLMETVSSLFQGKLNIL-------VNNVGTLMLKPTTEYTAEEFSFL 121

Query: 127 MSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLA 186
           M+TN +++FH SQLAHPL+KASG+GSIV MSS+AG                    + NLA
Sbjct: 122 MATNLDSAFHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLA 181

Query: 187 CEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSV-TPLKRAGEPHEVSSMVA 246
           CEWA DNIRTNA+ PW+I T    P   D + VE M       TP+ R GE +EVS +VA
Sbjct: 182 CEWASDNIRTNAICPWLITT----PLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVA 241

Query: 247 FLCLPTASYITGQVLYVDGGHTVNAF 252
           FLCLP ASYITGQV+ VDGG TVN F
Sbjct: 242 FLCLPAASYITGQVICVDGGLTVNGF 256

[Close]

BLAST of CmoCh06G002820 vs. TAIR 10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 268.5 bits (685), Expect = 5.6e-72
Identity = 143/266 (53.76%), Postives = 177/266 (66.54%), Query Frame = 0


Query: 9   RWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVSGS 68
           +WSL+GMTALVTGG+ G+G+A VEELA  GA VHTC+R +  L + L+EW+  G  V+ S
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTS 72

Query: 69  VCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSVMS 128
           VCDV SR+QR +LMETVSSLF G L+ILV        N G    K + + T EE S +++
Sbjct: 73  VCDVSSRDQREKLMETVSSLFQGKLSILVP-------NVGIGVLKPTTECTAEEFSFIIA 132

Query: 129 TNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLACE 188
           TN E++FHFSQLAHPL+KASG+G+IV MSSVAG                    + NLACE
Sbjct: 133 TNLESTFHFSQLAHPLLKASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACE 192

Query: 189 WAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFLC 248
           WA DNIR N+V PW I T   K    D    +V   + SVTPL+R GE +EVSS+VAFLC
Sbjct: 193 WASDNIRANSVCPWFITTPSTKDFLGDK---DVKEKVESVTPLRRVGEANEVSSLVAFLC 252

Query: 249 LPTASYITGQVLYVDGGHTVNAFPIP 255
           LP ASYITGQ + VDGG T+N F +P
Sbjct: 253 LPAASYITGQTICVDGGFTINGFSLP 268

[Close]

BLAST of CmoCh06G002820 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.3e-71
Identity = 144/264 (54.55%), Postives = 175/264 (66.29%), Query Frame = 0


Query: 8   QRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVSG 67
           +RWSL GM+ALVTGGT GIGRA VEELA  GA VHTC+R++ +L+ CL +W   GF V+G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 68  SVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSVM 127
           SVCDV  R QR  LMETVSS+F G L+IL       VNN GT   K   + T  E S++M
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHIL-------VNNVGTNIRKPMVEFTAGEFSTLM 183

Query: 128 STNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLAC 187
           STNFE+ FH  QLA+PL++ S  GS+VF+SSV+G                    + +LAC
Sbjct: 184 STNFESVFHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLAC 243

Query: 188 EWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAFL 247
           EWAKDNIR NAVAPW IKT  V+    +    E +  + SVTPL R GEP EVSS VAFL
Sbjct: 244 EWAKDNIRINAVAPWYIKTSMVEQVLSNK---EYLEEVYSVTPLGRLGEPREVSSAVAFL 303

Query: 248 CLPTASYITGQVLYVDGGHTVNAF 252
           CLP +SYITGQ+L VDGG ++N F
Sbjct: 304 CLPASSYITGQILCVDGGMSINGF 317

[Close]

BLAST of CmoCh06G002820 vs. TAIR 10
Match: AT2G29350.1 (senescence-associated gene 13 )

HSP 1 Score: 267.3 bits (682), Expect = 1.3e-71
Identity = 144/265 (54.34%), Postives = 176/265 (66.42%), Query Frame = 0


Query: 7   DQRWSLQGMTALVTGGTHGIGRATVEELAAFGATVHTCSRSQGDLDKCLKEWEEMGFNVS 66
           + RWSL GMTALVTGG+ GIG A VEELA  GA VHTC+R +  L + L+EW+  GF V+
Sbjct: 10  NSRWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLREWQAKGFQVT 69

Query: 67  GSVCDVHSREQRMQLMETVSSLFNGSLNILVTKMRKMVNNAGTWAYKNSEDVTEEELSSV 126
            SVCDV SR+QR++LMETVSSL+ G LNIL       VNN GT  +K + + T E+ S V
Sbjct: 70  TSVCDVSSRDQRVKLMETVSSLYQGKLNIL-------VNNVGTSIFKPTTEYTAEDFSFV 129

Query: 127 MSTNFEASFHFSQLAHPLMKASGNGSIVFMSSVAG--------------------SMNLA 186
           M+TN E++FH SQLAHPL+KASG+GSIV +SS AG                    + NLA
Sbjct: 130 MATNLESAFHLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATKGAMNQLARNLA 189

Query: 187 CEWAKDNIRTNAVAPWIIKTRFVKPPTDDPIHVEVMNHMLSVTPLKRAGEPHEVSSMVAF 246
           CEWA DNIRTN+V PW I T    P ++D    E     +  TP+ R GE +EVS +VAF
Sbjct: 190 CEWASDNIRTNSVCPWYITT----PLSNDFFDEEFKKEAVRTTPMGRVGEANEVSPLVAF 249

Query: 247 LCLPTASYITGQVLYVDGGHTVNAF 252
           LCLP+ASYITGQ + VDGG TVN F
Sbjct: 250 LCLPSASYITGQTICVDGGATVNGF 263

The following BLAST results are available for this feature:

BLAST of CmoCh06G002820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50165	9.7e-77	56.98	Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
Q9LHT0	2.9e-73	55.81	Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060...	[more]
Q9ZW13	5.5e-72	56.02	Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292...	[more]
Q9ZW20	7.9e-71	53.76	Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g293...	[more]
Q9ZW18	1.8e-70	54.34	Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 S...	[more]

BLAST of CmoCh06G002820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FN65	1.8e-134	90.22	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446688 PE=4 S...	[more]
A0A6J1FKX7	9.9e-125	84.42	tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111446681 PE=4 S...	[more]
A0A6J1I667	4.2e-123	84.06	tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111470275 PE=4 SV=...	[more]
A0A6J1CX69	2.5e-104	72.96	tropinone reductase homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CVA8	2.4e-102	68.64	tropinone reductase homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of CmoCh06G002820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06060.1	2.1e-74	55.81	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29290.2	3.9e-73	56.02	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	5.6e-72	53.76	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29260.1	1.3e-71	54.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29350.1	1.3e-71	54.34	senescence-associated gene 13	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 172..189 score: 36.49 coord: 16..33 score: 48.7 coord: 98..109 score: 37.73 coord: 210..230 score: 33.77
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 16..164 e-value: 8.7E-33 score: 113.4
None	No IPR available	GENE3D	3.40.50.720		coord: 1..251 e-value: 1.1E-66 score: 226.6
IPR045000	Tropinone reductase	PANTHER	PTHR42898	TROPINONE REDUCTASE	coord: 2..251
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 13..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G002820.1	CmoCh06G002820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016491	oxidoreductase activity