CmoCh06G000450 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh06G000450 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 530.0 bits (1364), Expect = 2.7e-149
Identity = 274/466 (58.80%), Postives = 341/466 (73.18%), Query Frame = 0

Query: 9   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 68
           MACNSS C+S CY  + D  +   D           A +G      +C KCK    M+  
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDD----------AVSGGGNHESVCVKCKCNAPMTFG 60

Query: 69  EEG-DDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQV 128
           + G DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG  SRV+LQFVHE+Q+
Sbjct: 61  DGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQI 120

Query: 129 KAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHI 188
           KA KN+EASRDRSL VFGVGVA++DET+A P    E+   I+ ++  VS LSPP KDLH+
Sbjct: 121 KALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHV 180

Query: 189 VQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTC 248
           V +ES++ S+  D R++L KLLD+V D TGKEDL+ HL+MLSLQK+AAENGYNRLVLG+C
Sbjct: 181 VPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSC 240

Query: 249 TSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEE 308
           TSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLRDC+  E+  LC  DGLKT E
Sbjct: 241 TSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVE 300

Query: 309 LTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATK 368
           L   S  GINDLVSSFVALLQEENPSRE TIVRTA KLTPF F++IP+ ++SN VP+AT+
Sbjct: 301 LACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSN-VPMATQ 360

Query: 369 RRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQIL 428
           RR KR N K+  S+++E FC +CNGPLN SD   L +FE GQ S +L   CCSSC+FQIL
Sbjct: 361 RRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCSSCRFQIL 420

Query: 429 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDE 474
           P+D  S E   S LP  M+++  H    +   LRE+I++ LL DDE
Sbjct: 421 PQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

BLAST of CmoCh06G000450 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 2.2e-106
Identity = 212/466 (45.49%), Postives = 292/466 (62.66%), Query Frame = 0

Query: 10  ACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSE 69
           +C  + C  +C  +    A  +  +    K  RL+ +         C KC       +  
Sbjct: 8   SCGGAGCGPHCSSSASAAAVEDAAAAAAEKVGRLSLSRE-------CGKCGGGAAAVAVA 67

Query: 70  EGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKA 129
            G     C +CFR+NL  KF+ AV SNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA
Sbjct: 68  GGLG--LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKA 127

Query: 130 HKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIVQ 189
            ++++ S  ++L VFGVGVA++DE+  C  P  EI+  I+ I+S+VSSLS  +K +HI +
Sbjct: 128 IESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIAR 187

Query: 190 IESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTS 249
           +E V+S+   DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY +++LG+C S
Sbjct: 188 LEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCAS 247

Query: 250 RIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEELT 309
            IACHV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDCLAQEL +LC  D LKT++  
Sbjct: 248 AIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHL 307

Query: 310 KTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRR 369
               +GIN LV+SF+  L+EENPSRE TIVRTA KL PF+F++       + +P  ++ R
Sbjct: 308 DRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLR 367

Query: 370 QKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRS--FEAGQVSSILAGTCCSSCQFQIL 429
            K   F    S  SE  C +C  P + S++Q L S   +A +   +    CC SC FQIL
Sbjct: 368 PKFQKFDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQIL 427

Query: 430 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDE 474
           P      E   SLLP     +    S S+S  LR+QI+E+LL +++
Sbjct: 428 PAGENLNEHFFSLLPKLWTGKMDTISDSHS-LLRDQIEEYLLEEND 461

BLAST of CmoCh06G000450 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 4.9e-106
Identity = 212/466 (45.49%), Postives = 292/466 (62.66%), Query Frame = 0

Query: 10  ACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSE 69
           +C  + C  +C  +    A  +  +    K  RL+ +         C KC       +  
Sbjct: 8   SCGGAGCGPHCSSSASAAAVEDAAAAAAEKVGRLSLSRE-------CGKCGGGAAAVAVA 67

Query: 70  EGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKA 129
            G     C +CFR+NL  KF+ AV SNAM+ P+D VL+AFSGGP SRVALQF+HEM+ KA
Sbjct: 68  GGLG--LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFIHEMRCKA 127

Query: 130 HKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIVQ 189
            ++++ S  ++L VFGVGVA++DE+  C  P  EI+  I+ I+S+VSSLS  +K +HIV+
Sbjct: 128 IESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGVKAMHIVR 187

Query: 190 IESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTS 249
           +E V+S+   DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY +++LG+C S
Sbjct: 188 LEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKIMLGSCAS 247

Query: 250 RIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEELT 309
            IACHV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDCLAQEL +LC  D LKT++  
Sbjct: 248 AIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDSLKTQQHL 307

Query: 310 KTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRR 369
               +GIN LV+SF+  L+EENPSRE TIVRTA KL PF+F++       + +P  ++ R
Sbjct: 308 DRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFLP--SRLR 367

Query: 370 QKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRS--FEAGQVSSILAGTCCSSCQFQIL 429
            K        S  SE  C +C  P + S++Q L S   +A +   +    CC SC FQIL
Sbjct: 368 PKFQKVDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQSCYFQIL 427

Query: 430 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDE 474
           P      E   SLLP     +    S S+S  LR+QI+E+LL +++
Sbjct: 428 PAGENLNEHFFSLLPKLWTGKMDTISDSHS-LLRDQIEEYLLEEND 461

BLAST of CmoCh06G000450 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.5e-22
Identity = 134/516 (25.97%), Postives = 209/516 (40.50%), Query Frame = 0

Query: 55  LCFKCKAKERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPC 114
           +C KCK          GD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 115 SRVALQFVHEMQVKAHKNFEASRDRSLQV-FGVGVAYIDETSACPIPLHEIDRTIQTIQS 174
           S   ++ V E           SRD   ++ F  G+ +IDE +AC +   E  + +  I S
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQQILSEICS 143

Query: 175 VVSSLSPPIKDLHIVQIESVYS---SNLSDG----------------------------- 234
           V+     P    HIV +E V+S   S L  G                             
Sbjct: 144 VLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 235 -------------------------------REKLKKLLDTVSDVTGKEDLISHLRMLSL 294
                                           +KLK+L  +   +T K+ L+  LR   +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 295 QKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLRDC 354
             IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R+ 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 355 LAQEL---NMLCCADGLKTEEL-TKTS-FSGINDLVSSFVALLQEENPSRESTIVRTAGK 414
            ++E+   N       +    L TK S  S I  L   FV  LQ + PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVSPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 415 LTPFNFSRI----------------PDLNESNGVPLATKRRQKRNNFKH-----VNSLSS 474
           L   N S+I                  L+   G   A    Q  ++         N L++
Sbjct: 384 L---NVSKIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLAN 443

Query: 475 ETFCAVCNGPLNMSDV---QTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSL 477
            +  + C G     +     T +S  A Q  S +    C SC+  +  KD  S    L +
Sbjct: 444 NSDKSCCQGGQGCKEAGYGDTCQS-RALQTPSFV-HMLCYSCRLTV--KDMQS----LDV 503

BLAST of CmoCh06G000450 vs. ExPASy Swiss-Prot
Match: Q28ES8 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Xenopus tropicalis OX=8364 GN=ctu2 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.7e-21
Identity = 135/530 (25.47%), Postives = 202/530 (38.11%), Query Frame = 0

Query: 56  CFKCKAKERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCS 115
           C KCK    +     GD   FC  CF+     KFR  +  N +I P +KVL+AFSGGP S
Sbjct: 25  CMKCKESSAVLLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAFSGGPSS 84

Query: 116 RVALQFVHEMQVKAHKNFEASRDRSLQV-FGVGVAYIDETSACPIPLHEIDRTIQTIQSV 175
              +Q V E           SRD   ++ F  G+ +IDE + C +   E  + +  +Q+V
Sbjct: 85  SAMVQQVQE---------GMSRDAPKKLRFVPGILFIDEGTTCGMSHEERQQNMSEVQNV 144

Query: 176 VSSLSPPIKDLHIVQIESVYS---------------------------------SNLSDG 235
           +     P    +IV +E V+S                                 +  SD 
Sbjct: 145 LQQTGFP---FYIVSLEQVFSLPGSILQRGVPEQRANYKQEVDRFMVQQQAQGETGCSDI 204

Query: 236 REKLKKLLDTVSD---------------------------------VTGKEDLISHLRML 295
            E L KL   VS+                                 +T K  L+  LR  
Sbjct: 205 LENLAKLGVNVSESSRESDKMFQSTCRHPPDTHTQKLIQLFASAKTLTAKHQLLHTLRSH 264

Query: 296 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARW-EVPVALPLR 355
            +  IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V +  P+R
Sbjct: 265 LILHIARTCGYSKVMSGESCTRLSVSLLSNISLGRGAFLPLDTGFCDSRYGDVDIIRPMR 324

Query: 356 DCLAQEL---NMLCCADGLKTEEL-TKT-SFSGINDLVSSFVALLQEENPSRESTIVRTA 415
           +   +E+   N L     +    L TK    S I  L   F+  LQ + PS  ST+ RT+
Sbjct: 325 EYSLKEIAFYNRLFHVSSVFIPALNTKVLENSSIQQLSEVFINRLQADFPSTVSTVYRTS 384

Query: 416 GKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTL 474
            KL   N S+I D N+        K R                 C +C  PL+ + V   
Sbjct: 385 EKL---NVSKI-DANQGT----CAKER-----------------CLLCLSPLD-TQVGEA 444

BLAST of CmoCh06G000450 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 9.1e-265
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 9   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 68
           MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 69  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 128
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 129 AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 188
           AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180

Query: 189 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 248
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 249 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEEL 308
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEEL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEEL 300

Query: 309 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 368
           TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR
Sbjct: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360

Query: 369 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 428
           RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 429 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 477
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

BLAST of CmoCh06G000450 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 1.6e-261
Identity = 463/468 (98.93%), Postives = 464/468 (99.15%), Query Frame = 0

Query: 9   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 68
           MACNSSACQSNCYRNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60

Query: 69  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 128
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVK 120

Query: 129 AHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 188
           AHKNFEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV
Sbjct: 121 AHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHIV 180

Query: 189 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 248
           QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT
Sbjct: 181 QIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCT 240

Query: 249 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEEL 308
           SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKT EL
Sbjct: 241 SRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTVEL 300

Query: 309 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 368
           TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR
Sbjct: 301 TKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKR 360

Query: 369 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 428
           RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP
Sbjct: 361 RQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILP 420

Query: 429 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 477
           KDSISQELLLSLLPPSMVARAMHGSPSNSNSLRE+IQEFLLSDDEVES
Sbjct: 421 KDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

BLAST of CmoCh06G000450 vs. ExPASy TrEMBL
Match: A0A6J1DEW9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111019863 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 401/476 (84.24%), Postives = 425/476 (89.29%), Query Frame = 0

Query: 9   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 68
           MACNSS CQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60

Query: 69  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 128
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 129 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 188
           VHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP HE+D  I+TIQSVVS+L PP
Sbjct: 121 VHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPSHEMDSAIETIQSVVSNLRPP 180

Query: 189 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 248
            K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL+SHLRMLSLQKIA ENGYNR
Sbjct: 181 SKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDLLSHLRMLSLQKIATENGYNR 240

Query: 249 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 308
           L++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC+AQEL+MLCC D
Sbjct: 241 LLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCIAQELHMLCCVD 300

Query: 309 GLKTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 368
           GLKT EL K SFSGIN LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDL+ESN 
Sbjct: 301 GLKTVELAKNSFSGINGLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLDESNN 360

Query: 369 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAG-QVSSILAGTCCS 428
           VPLATKRRQKRNN KHVNS+SSE+FCAVCN PLN+SDVQTLRS E   + ++IL+GT CS
Sbjct: 361 VPLATKRRQKRNNVKHVNSISSESFCAVCNSPLNISDVQTLRSCETSPETANILSGTWCS 420

Query: 429 SCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 477
           SCQFQILPKDS SQEL LSLLPPSMVARA+HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 421 SCQFQILPKDSTSQELFLSLLPPSMVARAIHGSTGNSNSLREQIQEFLLSDDEAES 475

BLAST of CmoCh06G000450 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.3e-215
Identity = 392/473 (82.88%), Postives = 417/473 (88.16%), Query Frame = 0

Query: 9   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-----TNGDSGDTHLCFKCKAKE 68
           MACNSSACQSNCYRNQ DE T+EQDS  +++    A      TN D+ +T+LCFKCK+KE
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESRITNDDNSNTNLCFKCKSKE 60

Query: 69  RMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVH 128
           RMS+S  GDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQFVH
Sbjct: 61  RMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFVH 120

Query: 129 EMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIK 188
           E+QVKAHKNFEASRDRSLQVFGVGVAYI+ET+A  IP  EID+TIQTIQSVVSSLS P K
Sbjct: 121 EIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQPRK 180

Query: 189 DLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLV 248
           DLHIV IE+VYSSNLSDGREKLKKLLD V DVTGKEDLIS+LRMLSLQKIAAENGYNRL+
Sbjct: 181 DLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNRLL 240

Query: 249 LGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGL 308
           +GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DCLAQELNMLCC DGL
Sbjct: 241 VGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCIDGL 300

Query: 309 KTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVP 368
           KT ELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN VP
Sbjct: 301 KTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVP 360

Query: 369 LATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQ 428
           LATKRRQKRNNFKHVNSLSSE+FCAVCNGPLN SDVQT  +      SS + G CCSSCQ
Sbjct: 361 LATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA------SSSILGACCSSCQ 420

Query: 429 FQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 477
           FQILPKDS+S EL LSLLPPSMVARA HGS  NSNSLR +IQEFLLSDD+ ES
Sbjct: 421 FQILPKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

BLAST of CmoCh06G000450 vs. ExPASy TrEMBL
Match: A0A5D3DPK4 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006240 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.3e-215
Identity = 392/473 (82.88%), Postives = 417/473 (88.16%), Query Frame = 0

Query: 9   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-----TNGDSGDTHLCFKCKAKE 68
           MACNSSACQSNCYRNQ DE T+EQDS  +++    A      TN D+ +T+LCFKCK+KE
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESRITNDDNSNTNLCFKCKSKE 60

Query: 69  RMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVH 128
           RMS+S  GDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQFVH
Sbjct: 61  RMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFVH 120

Query: 129 EMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIK 188
           E+QVKAHKNFEASRDRSLQVFGVGVAYI+ET+A  IP  EID+TIQTIQSVVSSLS P K
Sbjct: 121 EIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQPRK 180

Query: 189 DLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLV 248
           DLHIV IE+VYSSNLSDGREKLKKLLD V DVTGKEDLIS+LRMLSLQKIAAENGYNRL+
Sbjct: 181 DLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNRLL 240

Query: 249 LGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGL 308
           +GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DCLAQELNMLCC DGL
Sbjct: 241 VGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCIDGL 300

Query: 309 KTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVP 368
           KT ELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN VP
Sbjct: 301 KTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVP 360

Query: 369 LATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQ 428
           LATKRRQKRNNFKHVNSLSSE+FCAVCNGPLN SDVQT  +      SS + G CCSSCQ
Sbjct: 361 LATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA------SSSILGACCSSCQ 420

Query: 429 FQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 477
           FQILPKDS+S EL LSLLPPSMVARA HGS  NSNSLR +IQEFLLSDD+ ES
Sbjct: 421 FQILPKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

BLAST of CmoCh06G000450 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.9e-150
Identity = 274/466 (58.80%), Postives = 341/466 (73.18%), Query Frame = 0

Query: 9   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 68
           MACNSS C+S CY  + D  +   D           A +G      +C KCK    M+  
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDD----------AVSGGGNHESVCVKCKCNAPMTFG 60

Query: 69  EEG-DDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQV 128
           + G DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG  SRV+LQFVHE+Q+
Sbjct: 61  DGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQI 120

Query: 129 KAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPPIKDLHI 188
           KA KN+EASRDRSL VFGVGVA++DET+A P    E+   I+ ++  VS LSPP KDLH+
Sbjct: 121 KALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHV 180

Query: 189 VQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTC 248
           V +ES++ S+  D R++L KLLD+V D TGKEDL+ HL+MLSLQK+AAENGYNRLVLG+C
Sbjct: 181 VPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSC 240

Query: 249 TSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGLKTEE 308
           TSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLRDC+  E+  LC  DGLKT E
Sbjct: 241 TSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVE 300

Query: 309 LTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATK 368
           L   S  GINDLVSSFVALLQEENPSRE TIVRTA KLTPF F++IP+ ++SN VP+AT+
Sbjct: 301 LACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSN-VPMATQ 360

Query: 369 RRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQIL 428
           RR KR N K+  S+++E FC +CNGPLN SD   L +FE GQ S +L   CCSSC+FQIL
Sbjct: 361 RRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCSSCRFQIL 420

Query: 429 PKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDE 474
           P+D  S E   S LP  M+++  H    +   LRE+I++ LL DDE
Sbjct: 421 PQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455