CmoCh06G000010 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G000010
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	DNA-directed RNA polymerase V subunit 5A
Location	Cmo_Chr06: 3684 .. 7706 (+)
RNA-Seq Expression	CmoCh06G000010
Synteny	CmoCh06G000010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGTTTTTGTTTGTTGATTAGGTAGCCAACGGTGGTGTTCATCAACAACAAAATTATTTATTTATGTTCTCTCCATGAGGAGTTATTTACTGCTATTCCGGCCTTGTTTCTGTCCATAACCTCAAAATTTGGCTTACACCCTACCCCCAAATTCCATTAGTTCATCCCGAAAGGGTACCCACTTCAAAATCTTTCATTAAAATCTAGCTCTCAAAAATCACAAGTATTTAACTTAAGATATATATATATATAATTCCCCATTTTCAAACCAAGAAACGCGAAGCTGTATCCGGGAAGAGAGAGAGCGAAAATGGAGGAAGATGGGTTTTTGGGAGAACCGAATCGAGTGGAGCTAGGCACTTGCTTAAGTAGCTACATCGACGATGGCACCACTGAGAGCCATAGGTACTATCTTTCTCGCAGAACAGTCCTGGAGATGCTTAGGGACCGAGGCTACACCATCAACCCCTCTGATATCGACCTCACTCTCCAACAATTCCGAGAAATTTACGGCCAGTTCCTCGATGTCGATCGCCTTAAACTATCTGCTGCTCATCGCGCCGATCCCTCCAAAAGAGTAACACCCCACCACTTCTTCTTTTTTTCCCTTCGATTTATTTAAGCCTCGGTTGCTTAATTTGTTCTGCGGCGTCTTGTTGGTCATAGCAAATTAGGGTGGACAATGTGAGTACGAAAAGAGGTGCTGTAGTTTCTCATAGTATTTGGTTACTTCGAGAGCGTTTATTCGGCATGCTACTTTTGAATTTTGTTTGGAAGATAACGGTGCATGTTTTTTTCCTTCTTTTTTTTTATCATCGTTTAAATTCTGTGGACTAAACTTCTTACCTTCTTGGAAATTATTCGATGCAATCGTGCTTCTGAATTTTGAACACCTTATTTCACACCAAACACCTTATTTCACACCAATCTTTAGTAGTTTACGATTTATTATTGGCTTATGGAAGTATTTGTTGTGAATTGTGGTTCAACATTGATGCTGAGTGAAGTATTTTTCTATATGTGGACGAAACATAGAAATTATTGTACAATAGAAATATAGGTCTTAATGATGATGCTTCATAGTTATGCAAGTAGTCTAACATATTTAATTATTATTTCAAAACTGATCATTGTTTTTGCAGATTCTTGTAATATTCTGTGGGACTGGTGCAGTAAAGGTTAGTGCTATTCGCAATATTGCGGGCCAAATTGTTAACAAAGAAACTTTGCATGGTCTCATTCTAATTGTGCAAAATCAAATTACAAACCAAGCCATGAAAGCAGTTGAGGTCTTTTCGTTCAAGGTTGAAATATTCCAGGTTCGTTTCTTCAGTTTCTGATGTGATCTTCACAATAGACACTGCTGAGGTTGTTATCTAATTACAACAACATAAACTGGTTAAATTGAACATTAACTGCAACATGGGGAACCCGAACACTAAAAGGGTGGGGGAGTAAGAGTTTGTCATGGTAGAAAACAAAAATTACTTAGAAGAAGAAACTGAGGTCTCAAGATTTGATACAAGAGAGTCACCCTAAGAAAAATGAACCGCAGAAATAGGGGAAAGCTTGCAAACCTTTTTTTTTTTTTTTTTTTTTCCTTTCCTGAAAAAGGGTGATGAGAATCTTGTCATCTTATATATCTATTCCATAAATTTTCACTAAGAAATTTTTATAATGGTTATGTTCTCTGGGAGTGAATTGTGAGCTAGGGAAGTTGAATAGATGAACTGCTTTGGTTGGTTGTGAGGTTGCCTCTTTCCTGTCTTCCTATTAGATCTTCCCCTTGGTCATAACCCTTGTATATTGTCTTTCTGGGATCTTCTCATGAGTAAGATGCAAAAGCATTTAGCTTCTTGGAAAAGGTGTTCTTTCTCTCAAATGGGTCGGCTTACTCTCATTCAATCGGTGGTGTGGTATTCTGACTTGCTTTCTTTCCTTAAAGTCCTTGGAGAAGCTTGTGCGAGATTTCTCGTGGGAAGGGGTTGATGAGGGGAAGGGTTCCTATTTAGTTAGGTGGGAGGAGGTGTCCAACCCTGTGGTCCTCTGGGATTAGGCATATGGAACTTAAGGTTTTGTGTTGAGGTCGTCTTAGCTAAGTGGTTGTGGTGCTTTTCTACCTAACTCATGACTTCCTTTGACATAGGGTTATTGCAAGCAAGTATGGTTCTCATCTTCATGACTGCTCCTTGAACAAGGCTTTCAATAGGAACCCTTTGAAGGTCACTTCTAGGAACCCTTTGGCATAGGGAAACGATGGACATCATTAACGTAACCTCCATACTTCGGTTGAGGGTGTTTCGTTTCAGCCTGGAAGAGTGAATATTCGACTCTCGAGTCCTTGGATTTTAGAGGGGTTTTTTTTTTTCCTTGTAACTTTGAGTCTTTAGTAAACCCTTCTATGTCCTCTCATTCGGTCTTATACCACCTTAGATGTGAAATATGGAAAACATTTTGCACCCTGTTGTATGTGTAAGCTACAATCTATTAATGAAAACTGGATTTTGGAATTGATGCAGTAGTATTTATGAGAGTGGTTGCATGATGTAGAATGTTGCTTTAGACTACTAATGTAAGGCTAAGGGCTATCCATAATCTTAAAAGGTTCATATGTTGATTGAGTTTTTGTCTGTTACCAGATTACTGACTTGCTGGTTAATATCACAAAGCATGCATTAAAGCCAAACCATCAGGTGCTAACGGAGGAAGAAAAACAAAAGCTTTTGGAACAATACAGTATAGAGGAAAAACAGGTAAAATCTGTATAGATATTAGTCTGTGTCTGCAGTTGCCTTATGCTTTGTAAACCTTTGAAGAAGGCTTTTTAGGAAACAAAACCCCTTCTCATGGTAAACATGCTTTTCTTGAATGACCAACAATTTCTCCTTTCCAAGCCTTCATTGGTTCAGTTGTTGAGAGTTTGGTTTGTTCTTCCTGTATGAAAAATATGAATTCTGAAAACCTAATATATGTGGTATAAAAATGAATTGAAGTAGTGCAACAAATCTTGATATATATTTGTTAAACTACCAACATTTGCTTAATTAAGCCGACTTTTGATGTAATGCCAGCTTCCTCGTATGCTACGTACGGATGCCATTGCTCGATACTACGGACTCGAGAAGGGGCAGATTGTGAAAGTAACGTATGATGGTGACATGACTAGATCACATGTAACTTACCGTTGTGTCTGGTGATGGCCTATGAATGAATTCAGGTTTCACCAATTATGTTTTGTATTTTTAACCCCAAAGAACTTGATTACTTTTGTCTTAGACATCAATCAATGTTCCACAACTCCTTGTGTTACTAGATTTTCAGTGCATGTTTCGAGGCATTATTTATAATATACGTATCTCATTACTCTGCCAATACAACATTTGAAATATAAGTTGTACTAAATGTTTACAAGACGATTATTAAGATTTATTACAAACTAAACTCAAAAACTTGAATAGTTTTAATGAAATCACTAGAGTGCATCCAGACTATCTGCATCCGAGTTTTACAGGTAGTCGAGAAACCCGACACCTAAAAGGAAAACAATCAGTCATTCCAGCTGGACCAATCACGACTTCAAGTTCTGCTAGTGTTGCTAACAATACCGAAAACCAACCGAACAAAGAGTGTTCTTCTCCACCTATACGTACGCCCAAGGTTCTAACCAAAGCTGCATTGGTCAAATGTCAAGGAGATCATTTCATGGTCCAAAGCATTGCGGTAGAGACCTCTCCACAATCATCACTAACAATACATTCGACTCCATCAACTGGTTGGGATAGACAATTGCATCTGAAAGAGGCCGAGGTTGGCACAATGGTTGAACCAGAGGACTTGCTTACTACTGAAACTTCACCAGAGGGTGCGAAAGAAACACGTGTGAGAGAAGAATCACCAGTAGAATCACTGGACAAGGATACAGTCGAGAACAGTAGCTCTGCCGTGTTGTTGGATCGAAATCAGGTACATCTAATCTTAGTCATGACCATTCTGATCATCCCTTCTTTCCCTAGAAGAAACTGA

mRNA sequence

GGGTTTTTGTTTGTTGATTAGGTAGCCAACGGTGGTGTTCATCAACAACAAAATTATTTATTTATGTTCTCTCCATGAGGAGTTATTTACTGCTATTCCGGCCTTGTTTCTGTCCATAACCTCAAAATTTGGCTTACACCCTACCCCCAAATTCCATTAGTTCATCCCGAAAGGGTACCCACTTCAAAATCTTTCATTAAAATCTAGCTCTCAAAAATCACAAGTATTTAACTTAAGATATATATATATATAATTCCCCATTTTCAAACCAAGAAACGCGAAGCTGTATCCGGGAAGAGAGAGAGCGAAAATGGAGGAAGATGGGTTTTTGGGAGAACCGAATCGAGTGGAGCTAGGCACTTGCTTAAGTAGCTACATCGACGATGGCACCACTGAGAGCCATAGGTACTATCTTTCTCGCAGAACAGTCCTGGAGATGCTTAGGGACCGAGGCTACACCATCAACCCCTCTGATATCGACCTCACTCTCCAACAATTCCGAGAAATTTACGGCCAGTTCCTCGATGTCGATCGCCTTAAACTATCTGCTGCTCATCGCGCCGATCCCTCCAAAAGAATTCTTGTAATATTCTGTGGGACTGGTGCAGTAAAGGTTAGTGCTATTCGCAATATTGCGGGCCAAATTGTTAACAAAGAAACTTTGCATGGTCTCATTCTAATTGTGCAAAATCAAATTACAAACCAAGCCATGAAAGCAGTTGAGGTCTTTTCGTTCAAGGTTGAAATATTCCAGATTACTGACTTGCTGGTTAATATCACAAAGCATGCATTAAAGCCAAACCATCAGGTGCTAACGGAGGAAGAAAAACAAAAGCTTTTGGAACAATACAGTATAGAGGAAAAACAGCTTCCTCGTATGCTACGTACGGATGCCATTGCTCGATACTACGGACTCGAGAAGGGGCAGATTGTGAAAGTAACGTATGATGGTGACATGACTAGATCACATACATCAATCAATGTTCCACAACTCCTTGTGTTACTAGATTTTCAGTGCATGTTTCGAGGCATTATTTATAATATACGTATCTCATTACTCTGCCAATACAACATTTGAAATATAAGTTGTACTAAATGTTTACAAGACGATTATTAAGATTTATTACAAACTAAACTCAAAAACTTGAATAGTTTTAATGAAATCACTAGAGTGCATCCAGACTATCTGCATCCGAGTTTTACAGGTAGTCGAGAAACCCGACACCTAAAAGGAAAACAATCAGTCATTCCAGCTGGACCAATCACGACTTCAAGTTCTGCTAGTGTTGCTAACAATACCGAAAACCAACCGAACAAAGAGTGTTCTTCTCCACCTATACGTACGCCCAAGGTTCTAACCAAAGCTGCATTGGTCAAATGTCAAGGAGATCATTTCATGGTCCAAAGCATTGCGGTAGAGACCTCTCCACAATCATCACTAACAATACATTCGACTCCATCAACTGGTTGGGATAGACAATTGCATCTGAAAGAGGCCGAGGTTGGCACAATGGTTGAACCAGAGGACTTGCTTACTACTGAAACTTCACCAGAGGGTGCGAAAGAAACACGTGTGAGAGAAGAATCACCAGTAGAATCACTGGACAAGGATACAGTCGAGAACAGTAGCTCTGCCGTGTTGTTGGATCGAAATCAGGTACATCTAATCTTAGTCATGACCATTCTGATCATCCCTTCTTTCCCTAGAAGAAACTGA

Coding sequence (CDS)

ATGGAGGAAGATGGGTTTTTGGGAGAACCGAATCGAGTGGAGCTAGGCACTTGCTTAAGTAGCTACATCGACGATGGCACCACTGAGAGCCATAGGTACTATCTTTCTCGCAGAACAGTCCTGGAGATGCTTAGGGACCGAGGCTACACCATCAACCCCTCTGATATCGACCTCACTCTCCAACAATTCCGAGAAATTTACGGCCAGTTCCTCGATGTCGATCGCCTTAAACTATCTGCTGCTCATCGCGCCGATCCCTCCAAAAGAATTCTTGTAATATTCTGTGGGACTGGTGCAGTAAAGGTTAGTGCTATTCGCAATATTGCGGGCCAAATTGTTAACAAAGAAACTTTGCATGGTCTCATTCTAATTGTGCAAAATCAAATTACAAACCAAGCCATGAAAGCAGTTGAGGTCTTTTCGTTCAAGGTTGAAATATTCCAGATTACTGACTTGCTGGTTAATATCACAAAGCATGCATTAAAGCCAAACCATCAGGTGCTAACGGAGGAAGAAAAACAAAAGCTTTTGGAACAATACAGTATAGAGGAAAAACAGCTTCCTCGTATGCTACGTACGGATGCCATTGCTCGATACTACGGACTCGAGAAGGGGCAGATTGTGAAAGTAACGTATGATGGTGACATGACTAGATCACATACATCAATCAATGTTCCACAACTCCTTGTGTTACTAGATTTTCAGTGCATGTTTCGAGGCATTATTTATAATATACGTATCTCATTACTCTGCCAATACAACATTTGA

Protein sequence

MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHTSINVPQLLVLLDFQCMFRGIIYNIRISLLCQYNI

Homology

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BLAST of CmoCh06G000010 vs. ExPASy Swiss-Prot
Match: Q9M1J2 (DNA-directed RNA polymerase V subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPE5A PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 2.7e-71
Identity = 127/205 (61.95%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 18  CLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLK 77
           CLS Y+D  + ESHRYYL+RR  L+MLRDRGY ++  DI+L+L  FR +YG+  DVDRL+
Sbjct: 13  CLSKYVDLSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLR 72

Query: 78  LSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAV 137
           +SA HR+D +K++ ++F GT  VKV+AIR++   I+++ET+ GLIL++QN +TNQA+KA+
Sbjct: 73  ISALHRSDSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAI 132

Query: 138 EVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIA 197
           E+FSFKVEIFQITDLLVNITKH+LKP HQVL +EEK  LL+++SIEEKQLPR+ + DAI 
Sbjct: 133 ELFSFKVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIV 192

Query: 198 RYYGLEKGQIVKVTYDGDMTRSHTS 223
           RYYGLEKGQ+VKV Y G++T SH +
Sbjct: 193 RYYGLEKGQVVKVNYRGELTESHVA 217

[Close]

BLAST of CmoCh06G000010 vs. ExPASy Swiss-Prot
Match: Q9ZVB9 (DNA-directed RNA polymerases IV and V subunit 5B OS=Arabidopsis thaliana OX=3702 GN=NRPD5B PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 7.7e-66
Identity = 118/200 (59.00%), Postives = 162/200 (81.00%), Query Frame = 0


Query: 23  IDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLKLSAAH 82
           I   + E H+YYL+RRT +EMLRDRGY ++  DI+L+LQQFR +YG+  DVD L++SA H
Sbjct: 14  ISKSSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKH 73

Query: 83  RADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAVEVFSF 142
           R D SK+I V+FCGTG VKV+A+R IA  ++++E + GLIL++Q+ ITNQA+KAVE+FSF
Sbjct: 74  RFDSSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAVELFSF 133

Query: 143 KVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIARYYGL 202
           KVE+F+ITDLLVN++KH L+P HQVL ++EK+ LL+++SIEEKQLPR+   D I RYYGL
Sbjct: 134 KVELFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQLPRLSSKDPIVRYYGL 193

Query: 203 EKGQIVKVTYDGDMTRSHTS 223
           E GQ++KVTY  +++ SH +
Sbjct: 194 ETGQVMKVTYKDELSESHVT 213

[Close]

BLAST of CmoCh06G000010 vs. ExPASy Swiss-Prot
Match: Q9M1H8 (DNA-directed RNA polymerase V subunit 5C OS=Arabidopsis thaliana OX=3702 GN=NRPE5C PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.0e-46
Identity = 90/195 (46.15%), Postives = 137/195 (70.26%), Query Frame = 0


Query: 18  CLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLK 77
           C+S   D G  ES R+YL+R T  EMLRDRGY +N +++ LTL +FR ++G+  +++RL+
Sbjct: 24  CISKTEDTGGIESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLR 83

Query: 78  LSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAV 137
           +    R+DP K+ILV+F GT  + V ++R +  QI N   LH +IL++Q+++ + A KA+
Sbjct: 84  ICVPLRSDPKKKILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQKAL 143

Query: 138 EVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIA 197
             F F VE F I DLLVNITKH  +P  ++L +EEK++LL ++++E+KQLP +   D+  
Sbjct: 144 TTFPFTVETFPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFV 203

Query: 198 RYYGLEKGQIVKVTY 213
           RYYGL+K Q+VK+TY
Sbjct: 204 RYYGLKKKQVVKITY 218

[Close]

BLAST of CmoCh06G000010 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 7.5e-29
Identity = 74/184 (40.22%), Postives = 112/184 (60.87%), Query Frame = 0


Query: 29  ESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLKLSAAHRADPSK 88
           E  R Y  ++T+++MLRDRGY I  S++ +T QQF   +G  +  + L    A R D S 
Sbjct: 6   ELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSD 65

Query: 89  RILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAVEVFS--FKVEI 148
           ++ + F     V V  ++    + +  E +   IL+VQ  +T  A   +   S  F +E+
Sbjct: 66  QLYIFFPDEAKVGVKTMKMYTNR-MKSENVFRAILVVQQNLTPFARTCISEISSKFHLEV 125

Query: 149 FQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIARYYGLEKGQ 208
           FQ  ++LVNI +H L P HQVLT EEK+ LLE+Y+++E QLPR+  TD IARY+GL++GQ
Sbjct: 126 FQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKRGQ 185

Query: 209 IVKV 211
           +VK+
Sbjct: 186 VVKI 188

[Close]

BLAST of CmoCh06G000010 vs. ExPASy Swiss-Prot
Match: A8XGH1 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis briggsae OX=6238 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.3e-25
Identity = 65/193 (33.68%), Postives = 114/193 (59.07%), Query Frame = 0


Query: 25  DGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDR-----LKLS 84
           D   E++R +  R+TVL+M+ DRGY +   ++D  L+ F+E +G      +     L + 
Sbjct: 3   DDELETYRLWRIRKTVLQMVHDRGYLVAQEELDQPLETFKETFGDRPSEKKPARSDLTIL 62

Query: 85  AAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAVEV 144
            AH  DP+ ++ V F     + +  I+ I  Q + ++ +   I++VQ  +T  A +++  
Sbjct: 63  VAHNDDPADQMFVFFPEDTKIGIKTIKAICQQ-MQEQNISRAIIVVQTGMTPSAKQSIGD 122

Query: 145 FS--FKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIA 204
            +  + +E F   +L+VNIT+H L P H V+T EEK +LL +Y +++ QLPR+ + D +A
Sbjct: 123 MAPKYMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPVA 182

Query: 205 RYYGLEKGQIVKV 211
           RY+GL +GQ+VK+
Sbjct: 183 RYFGLRRGQVVKI 194

[Close]

BLAST of CmoCh06G000010 vs. ExPASy TrEMBL
Match: A0A6J1GHB9 (DNA-directed RNA polymerase V subunit 5A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453892 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 9.0e-118
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60
           MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120
           QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG
Sbjct: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180
           LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHTS 223
           SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSH +
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHVT 222

[Close]

BLAST of CmoCh06G000010 vs. ExPASy TrEMBL
Match: A0A6J1GH86 (DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453892 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 9.0e-118
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60
           MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120
           QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG
Sbjct: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180
           LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHTS 223
           SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSH +
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHVT 222

[Close]

BLAST of CmoCh06G000010 vs. ExPASy TrEMBL
Match: A0A6J1I733 (DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470215 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.9e-116
Identity = 218/222 (98.20%), Postives = 219/222 (98.65%), Query Frame = 0


Query: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60
           MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120
           QQFREIYGQF DVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG
Sbjct: 61  QQFREIYGQFPDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180
           LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHTS 223
           SIEEKQLPRMLRTDAIARYY LEKGQIVKVTYDGDMTRSH +
Sbjct: 181 SIEEKQLPRMLRTDAIARYYRLEKGQIVKVTYDGDMTRSHVT 222

[Close]

BLAST of CmoCh06G000010 vs. ExPASy TrEMBL
Match: A0A6J1I260 (DNA-directed RNA polymerase V subunit 5A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470215 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.9e-116
Identity = 218/222 (98.20%), Postives = 219/222 (98.65%), Query Frame = 0


Query: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60
           MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120
           QQFREIYGQF DVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG
Sbjct: 61  QQFREIYGQFPDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180
           LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHTS 223
           SIEEKQLPRMLRTDAIARYY LEKGQIVKVTYDGDMTRSH +
Sbjct: 181 SIEEKQLPRMLRTDAIARYYRLEKGQIVKVTYDGDMTRSHVT 222

[Close]

BLAST of CmoCh06G000010 vs. ExPASy TrEMBL
Match: A0A6J1E191 (DNA-directed RNA polymerase V subunit 5A OS=Momordica charantia OX=3673 GN=LOC111025016 PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.7e-106
Identity = 199/222 (89.64%), Postives = 211/222 (95.05%), Query Frame = 0


Query: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60
           MEEDGF GEPNRVELGTCLSSYID+G+TESHRY+L RRTVLEMLRDRGYT   SD DLTL
Sbjct: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60

Query: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120
           QQFREIYGQ  DVDRLKLSA HR+DPSKRILVIFCGTG+VKV+AIRNIAGQIVNK+TLHG
Sbjct: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120

Query: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180
           LILIVQNQITNQAMKAV++FSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLL+QY
Sbjct: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHTS 223
           SIEEKQLPRMLRTDAIARYYGLEKGQ+VKVTYDGDMT SH +
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVT 222

[Close]

BLAST of CmoCh06G000010 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 270.0 bits (689), Expect = 1.9e-72
Identity = 127/205 (61.95%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 18  CLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLK 77
           CLS Y+D  + ESHRYYL+RR  L+MLRDRGY ++  DI+L+L  FR +YG+  DVDRL+
Sbjct: 13  CLSKYVDLSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLR 72

Query: 78  LSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAV 137
           +SA HR+D +K++ ++F GT  VKV+AIR++   I+++ET+ GLIL++QN +TNQA+KA+
Sbjct: 73  ISALHRSDSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAI 132

Query: 138 EVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIA 197
           E+FSFKVEIFQITDLLVNITKH+LKP HQVL +EEK  LL+++SIEEKQLPR+ + DAI 
Sbjct: 133 ELFSFKVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIV 192

Query: 198 RYYGLEKGQIVKVTYDGDMTRSHTS 223
           RYYGLEKGQ+VKV Y G++T SH +
Sbjct: 193 RYYGLEKGQVVKVNYRGELTESHVA 217

[Close]

BLAST of CmoCh06G000010 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 251.9 bits (642), Expect = 5.4e-67
Identity = 118/200 (59.00%), Postives = 162/200 (81.00%), Query Frame = 0


Query: 23  IDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLKLSAAH 82
           I   + E H+YYL+RRT +EMLRDRGY ++  DI+L+LQQFR +YG+  DVD L++SA H
Sbjct: 14  ISKSSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKH 73

Query: 83  RADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAVEVFSF 142
           R D SK+I V+FCGTG VKV+A+R IA  ++++E + GLIL++Q+ ITNQA+KAVE+FSF
Sbjct: 74  RFDSSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAVELFSF 133

Query: 143 KVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIARYYGL 202
           KVE+F+ITDLLVN++KH L+P HQVL ++EK+ LL+++SIEEKQLPR+   D I RYYGL
Sbjct: 134 KVELFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQLPRLSSKDPIVRYYGL 193

Query: 203 EKGQIVKVTYDGDMTRSHTS 223
           E GQ++KVTY  +++ SH +
Sbjct: 194 ETGQVMKVTYKDELSESHVT 213

[Close]

BLAST of CmoCh06G000010 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 188.3 bits (477), Expect = 7.4e-48
Identity = 90/195 (46.15%), Postives = 137/195 (70.26%), Query Frame = 0


Query: 18  CLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLK 77
           C+S   D G  ES R+YL+R T  EMLRDRGY +N +++ LTL +FR ++G+  +++RL+
Sbjct: 24  CISKTEDTGGIESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLR 83

Query: 78  LSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAV 137
           +    R+DP K+ILV+F GT  + V ++R +  QI N   LH +IL++Q+++ + A KA+
Sbjct: 84  ICVPLRSDPKKKILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQKAL 143

Query: 138 EVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIA 197
             F F VE F I DLLVNITKH  +P  ++L +EEK++LL ++++E+KQLP +   D+  
Sbjct: 144 TTFPFTVETFPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFV 203

Query: 198 RYYGLEKGQIVKVTY 213
           RYYGL+K Q+VK+TY
Sbjct: 204 RYYGLKKKQVVKITY 218

[Close]

BLAST of CmoCh06G000010 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 129.0 bits (323), Expect = 5.3e-30
Identity = 74/184 (40.22%), Postives = 112/184 (60.87%), Query Frame = 0


Query: 29  ESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFLDVDRLKLSAAHRADPSK 88
           E  R Y  ++T+++MLRDRGY I  S++ +T QQF   +G  +  + L    A R D S 
Sbjct: 6   ELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSD 65

Query: 89  RILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAVEVFS--FKVEI 148
           ++ + F     V V  ++    + +  E +   IL+VQ  +T  A   +   S  F +E+
Sbjct: 66  QLYIFFPDEAKVGVKTMKMYTNR-MKSENVFRAILVVQQNLTPFARTCISEISSKFHLEV 125

Query: 149 FQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIARYYGLEKGQ 208
           FQ  ++LVNI +H L P HQVLT EEK+ LLE+Y+++E QLPR+  TD IARY+GL++GQ
Sbjct: 126 FQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKRGQ 185

Query: 209 IVKV 211
           +VK+
Sbjct: 186 VVKI 188

[Close]

BLAST of CmoCh06G000010 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 115.5 bits (288), Expect = 6.1e-26
Identity = 70/193 (36.27%), Postives = 115/193 (59.59%), Query Frame = 0


Query: 23  IDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTLQQFREIYGQFL---DVDRLKLS 82
           + D   E  R +  RRTVL+MLRDRGYTI  SD++L  ++F + + + +   + + L +S
Sbjct: 1   MSDMDDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVS 60

Query: 83  AAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHGLILIVQNQITNQAMKAVEV 142
           A    +P+ +I V +     V V  I+      +  + +H  I++V   IT  A  AV  
Sbjct: 61  ANKGPNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSE 120

Query: 143 FS--FKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQYSIEEKQLPRMLRTDAIA 202
            +    +E+F+  +L+ NIT+H L   + VL ++ K+KLL  Y++++ QLPR+L TD +A
Sbjct: 121 LNKMLTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLA 180

Query: 203 RYYGLEKGQIVKV 211
           RYYGL++GQ+VK+
Sbjct: 181 RYYGLKRGQVVKI 193

The following BLAST results are available for this feature:

BLAST of CmoCh06G000010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1J2	2.7e-71	61.95	DNA-directed RNA polymerase V subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPE...	[more]
Q9ZVB9	7.7e-66	59.00	DNA-directed RNA polymerases IV and V subunit 5B OS=Arabidopsis thaliana OX=3702...	[more]
Q9M1H8	1.0e-46	46.15	DNA-directed RNA polymerase V subunit 5C OS=Arabidopsis thaliana OX=3702 GN=NRPE...	[more]
O81098	7.5e-29	40.22	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
A8XGH1	1.3e-25	33.68	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis bri...	[more]

BLAST of CmoCh06G000010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GHB9	9.0e-118	99.10	DNA-directed RNA polymerase V subunit 5A isoform X1 OS=Cucurbita moschata OX=366...	[more]
A0A6J1GH86	9.0e-118	99.10	DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita moschata OX=366...	[more]
A0A6J1I733	4.9e-116	98.20	DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1I260	4.9e-116	98.20	DNA-directed RNA polymerase V subunit 5A isoform X1 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1E191	2.7e-106	89.64	DNA-directed RNA polymerase V subunit 5A OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of CmoCh06G000010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57080.1	1.9e-72	61.95	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT2G41340.1	5.4e-67	59.00	RNA polymerase II fifth largest subunit, D	[more]
AT3G54490.1	7.4e-48	46.15	RNA polymerase II fifth largest subunit, E	[more]
AT3G22320.1	5.3e-30	40.22	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	6.1e-26	36.27	RNA polymerase II fifth largest subunit, C	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 24..151 e-value: 4.7E-35 score: 122.5
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 28..155
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 154..219 e-value: 2.6E-25 score: 88.1
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 28..111 e-value: 1.1E-21 score: 77.3
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 152..223 e-value: 5.0E-23 score: 82.5
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 152..220
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 10..222
None	No IPR available	PANTHER	PTHR10535:SF2	DNA-DIRECTED RNA POLYMERASE V SUBUNIT 5A	coord: 10..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G000010.1	CmoCh06G000010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0055029	nuclear DNA-directed RNA polymerase complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity