CmoCh05G009470 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh05G009470
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Terpene cyclase/mutase family member
Location	Cmo_Chr05: 7562425 .. 7564276 (-)
RNA-Seq Expression	CmoCh05G009470
Synteny	CmoCh05G009470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAGACTTACGGTGGGAGAAGGAGAAAATAATCCATACTTATTCAGCTCCAACAACTTTGTGGGAAGACAAACGTGGGACTTCGAGCCCGATGAAGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCCCGCCAAAATTATTACCATAATCATTTCAAAGTCCCATGCAACAATGACCTATTTTAGAAATTTCAGGTTCTCTTTAATTCTTATATAAATTCTTTTAATCATGTTTATAGAAATAACTAGAATTTTTTTTAAATAAATGTTTGAAGATGTTTTCAACTAACTTATTTAGAACGAATTAGATAAATGTATTTTAAATAAATGTTTGAAGATGCGCGCAAGTATTTGTTAAATGAATAAATACGAGTGGGGTTTTGCGTACCAAGTATTTGTTAAATGAATAAATACGAGTGGGGTTAGAATAAATTGTCCTTTATTTTCAACTCATTTATTTATAAAGAATTGACCTAAACACATATTTATTTAGTCTACTTTATTAATAAATGTGTTTAACAAGTTATTGAATATATGCTCAATAATTTTCTTCATTATGATGACGTAGTTTCTAAGAGACAAAAATTTCAAACAAACAATTCCAATAGTGAGAGTGAAGGAAGGAAGAGATGTGGAAGAGAAAATAGCAATAATTAAGAAGGAAACAGCGTCCATTGCACTCAGACGAGCCACCAGCTTCTTTGCAGCCTTACAAAGCCACCATGGTCATTGGCCTGCTGAAAACGCTGGCCCTAAGTTTTATTTCCCTCCACTGGTACTCTTCTTTTTCTATTCATTTTATCTGGTATAATTCTACCACAAATTTACCATTTTCTTTTCAGGTATTTTCGTTGTATACTACGGGACATCTTAGCATCATATTCTCAGAACATCACCGAAAGGAAATCCTTCGTTATTCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTTCGTTTGTTGGGAGAAGAGCATGACAAAGATGAGTGTTTTAAAGCTAGAAAATGGATTCTTGATCTTGGAGGTGCTCTTTACATACCCTCTTGGGGAAAGATTTGGCTTTGTGTTAGTCCCATATATATCTCTACACTATCTGATTTGAAACCACGTAACTCAAATTTAAGAAACTAAATTTATAATTTAACTATGTAGATTCTCGGAGTTTGTGAGTGGAAGGGAGCAAATCCCATGCCTCCAAAATTTTGGTTGTTAGGGAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCTAGATTGACACTTCTTCCTATGTCGTATTTATTTGGGAAGCAATTCGTTGGACCCCTCACATCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAACCACATTAAATGGAGTCCTACCCGCCATTATTGTGCAAAGGTACGTTCCTAACCCATTCAATCAATATCCATCCTTTCAAAAGGTTTCAAATTTGTACATGTAGAAATTGAATATTTCTATTTCATGTACGATATGAGACTTTGATTATTTTGATTGATTGGACATGATCATGCAGGAAGACAAATGCTTTGAACGTTCATTATTTCAAAAGCTAGCATGAGATACTCTTCACTACCTTGGAGAACCCATTCTTAATAGTTCGGCCTTTAAAAAGATAAGAAATAGAGCTCTTCAAGTAGCTAAATCTCACATTGATTATGAAGATCATAATAGTCATTACATTACAATGGGATGCGTTGAAAAGGTTAATTTCATACTTCTTTTTTATTTTATTATTATCATTATAGTTAATAATATTACATTAA

mRNA sequence

ATGTGGAGACTTACGGTGGGAGAAGGAGAAAATAATCCATACTTATTCAGCTCCAACAACTTTGTGGGAAGACAAACGTGGGACTTCGAGCCCGATGAAGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCCCGCCAAAATTATTACCATAATCATTTCAAATTTCTAAGAGACAAAAATTTCAAACAAACAATTCCAATAGTGAGAGTGAAGGAAGGAAGAGATGTGGAAGAGAAAATAGCAATAATTAAGAAGGAAACAGCGTCCATTGCACTCAGACGAGCCACCAGCTTCTTTGCAGCCTTACAAAGCCACCATGGTCATTGGCCTGCTGAAAACGCTGGCCCTAAGTTTTATTTCCCTCCACTGGTATTTTCGTTGTATACTACGGGACATCTTAGCATCATATTCTCAGAACATCACCGAAAGGAAATCCTTCGTTATTCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTTCGTTTGTTGGGAGAAGAGCATGACAAAGATGAGTGTTTTAAAGCTAGAAAATGGATTCTTGATCTTGGAGGTGCTCTTTACATACCCTCTTGGGGAAAGATTTGGCTTTGTATTCTCGGAGTTTGTGAGTGGAAGGGAGCAAATCCCATGCCTCCAAAATTTTGGTTGTTAGGGAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCTAGATTGACACTTCTTCCTATGTCGTATTTATTTGGGAAGCAATTCGTTGGACCCCTCACATCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAACCACATTAAATGGAGTCCTACCCGCCATTATTGTGCAAAGATAAGAAATAGAGCTCTTCAAGTAGCTAAATCTCACATTGATTATGAAGATCATAATAGTCATTACATTACAATGGGATGCGTTGAAAAGTTAATAATATTACATTAA

Coding sequence (CDS)

ATGTGGAGACTTACGGTGGGAGAAGGAGAAAATAATCCATACTTATTCAGCTCCAACAACTTTGTGGGAAGACAAACGTGGGACTTCGAGCCCGATGAAGGCACTCCAGAAGAGCGAGCTGAAGTGGAAGAAGCCCGCCAAAATTATTACCATAATCATTTCAAATTTCTAAGAGACAAAAATTTCAAACAAACAATTCCAATAGTGAGAGTGAAGGAAGGAAGAGATGTGGAAGAGAAAATAGCAATAATTAAGAAGGAAACAGCGTCCATTGCACTCAGACGAGCCACCAGCTTCTTTGCAGCCTTACAAAGCCACCATGGTCATTGGCCTGCTGAAAACGCTGGCCCTAAGTTTTATTTCCCTCCACTGGTATTTTCGTTGTATACTACGGGACATCTTAGCATCATATTCTCAGAACATCACCGAAAGGAAATCCTTCGTTATTCATATTGTCATCAGAATGAAGATGGTGGATGGGGATTGCACATTGAGGGACAAAGTTGTATGCTTTGCACTGTTTTTAACTATATTCAACTTCGTTTGTTGGGAGAAGAGCATGACAAAGATGAGTGTTTTAAAGCTAGAAAATGGATTCTTGATCTTGGAGGTGCTCTTTACATACCCTCTTGGGGAAAGATTTGGCTTTGTATTCTCGGAGTTTGTGAGTGGAAGGGAGCAAATCCCATGCCTCCAAAATTTTGGTTGTTAGGGAAATTACTTCCTTTCATTCCTCGAACTTTGTTTTGCTATTCTAGATTGACACTTCTTCCTATGTCGTATTTATTTGGGAAGCAATTCGTTGGACCCCTCACATCTCTCATTCTTCAATTACGCCAAGAAATCTATACTCAACCTTACAACCACATTAAATGGAGTCCTACCCGCCATTATTGTGCAAAGATAAGAAATAGAGCTCTTCAAGTAGCTAAATCTCACATTGATTATGAAGATCATAATAGTCATTACATTACAATGGGATGCGTTGAAAAGTTAATAATATTACATTAA

Protein sequence

MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFKFLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHGHWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQSCMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYNHIKWSPTRHYCAKIRNRALQVAKSHIDYEDHNSHYITMGCVEKLIILH

Homology

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BLAST of CmoCh05G009470 vs. ExPASy Swiss-Prot
Match: E7DN63 (Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 1.9e-117
Identity = 202/383 (52.74%), Postives = 254/383 (66.32%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MW+L + EG+N PYL+S+NN+VGRQTW+F+P+ GT EERA++EEARQ +++N +K     
Sbjct: 1   MWKLKIAEGQNGPYLYSTNNYVGRQTWEFDPNGGTIEERAKIEEARQQFWNNRYKVKPSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FL +KNFKQ IP V+V+EG +       I  E A+IAL RA +FF+ALQ+  G
Sbjct: 61  DLLWRIQFLGEKNFKQKIPAVKVEEGEE-------ISHEVATIALHRAVNFFSALQATDG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAENAGP F+ PPLV  +Y TGHL+ +F   HRKEILRY YCHQNEDGGWGLHIEG S
Sbjct: 121 HWPAENAGPLFFLPPLVMCMYITGHLNTVFPAEHRKEILRYIYCHQNEDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEHD---KDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWK 240
            M CT  +YI +R+LGE  D    + C +ARKWILD G    IPSWGK WL ILGV EW 
Sbjct: 181 TMFCTALSYICMRILGEGPDGGVNNACARARKWILDHGSVTAIPSWGKTWLSILGVFEWI 240

Query: 241 GANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQ 300
           G NPMPP+FW+L   LP  P  ++CY R+  +PMSYL+GK+FVGP+T LILQLR+E+Y +
Sbjct: 241 GTNPMPPEFWILPSFLPVHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYDR 300

Query: 301 PYNHIKWSPTRHYCA---------------------------------KIRNRALQVAKS 336
           PY+ I W   RH CA                                 K+RN+AL+V   
Sbjct: 301 PYDEINWKKVRHVCAKEDLYYPHPLVQDLMWDSLYICTEPLLTRWPFNKLRNKALEVTMK 360

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BLAST of CmoCh05G009470 vs. ExPASy Swiss-Prot
Match: Q2XPU7 (Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 4.3e-117
Identity = 203/379 (53.56%), Postives = 250/379 (65.96%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHF------ 60
           MWR+ + EG NNPY++S+NNF GRQ W F+P+ GTPEE+AEVEEARQN++ N F      
Sbjct: 1   MWRIKIAEGGNNPYIYSTNNFQGRQIWVFDPNAGTPEEQAEVEEARQNFWKNRFQVKPNS 60

Query: 61  ------KFLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                 +FLR+KNFKQ IP V+V++G +       I  E A+ ALRR+   F+ALQ+  G
Sbjct: 61  DLLWQLQFLREKNFKQKIPKVKVEDGEE-------ITSEIAAAALRRSVHLFSALQASDG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HW AEN G  F+ PPLVF++Y TGHL+ +FS  HRKEILRY YCHQNEDGGWG+HIEG S
Sbjct: 121 HWCAENGGLLFFLPPLVFAVYITGHLNTVFSPEHRKEILRYIYCHQNEDGGWGIHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEHD---KDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWK 240
            M CTV NYI +R+LGE  D   ++ C + RKWILD GGA  I SWGK WL ILGV EW 
Sbjct: 181 TMFCTVLNYICMRILGEARDGGIENACERGRKWILDHGGATGISSWGKTWLSILGVYEWD 240

Query: 241 GANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQ 300
           G NPMPP+FW      P  P  +FCY R+T +PMSYL+GK+FVGP+T LILQ+R+EIY +
Sbjct: 241 GTNPMPPEFWAFPSSFPLHPAKMFCYCRITYMPMSYLYGKRFVGPITPLILQIREEIYNE 300

Query: 301 PYNHIKWSPTRHYCA---------------------------------KIRNRALQVAKS 332
           PYN IKW+  RH CA                                 K+R +AL++   
Sbjct: 301 PYNKIKWNSVRHLCAKEDNYFPHPTIQKLLWDALYTFSEPLFSRWPFNKLREKALKITMD 360

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BLAST of CmoCh05G009470 vs. ExPASy Swiss-Prot
Match: Q8W3Z1 (Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 7.4e-117
Identity = 198/384 (51.56%), Postives = 257/384 (66.93%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHF------ 60
           MWRL + +G ++PY++S+NNFVGRQTW+F+P  G+P+ERAEVEEAR+N+Y N +      
Sbjct: 1   MWRLKIADGGSDPYIYSTNNFVGRQTWEFDPQAGSPQERAEVEEARRNFYDNRYQVKPSG 60

Query: 61  ------KFLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                 +FL++KNFKQTIP V+V++G +       I  E ++ ALRRA  F++ALQ+  G
Sbjct: 61  DLLWRMQFLKEKNFKQTIPPVKVEDGEE-------ITYEKSTAALRRAVHFYSALQASDG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAENAGP F+ PPLV  +Y TGHL+ +F   H+KEILRY Y HQNEDGGWGLHIEG S
Sbjct: 121 HWPAENAGPLFFLPPLVMCMYITGHLNTVFPAEHQKEILRYIYYHQNEDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEHD---KDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWK 240
            M CT  +YI +R+LGE  D    + C +ARKWILD GG  ++PSWGK WL ILG+ EW 
Sbjct: 181 TMFCTALSYICMRILGEGPDGGQDNACARARKWILDHGGVTHMPSWGKTWLSILGIFEWI 240

Query: 241 GANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQ 300
           G+NPMPP+FW+L   LP  P  ++CY R+  +PMSYL+GK+FVGP+T LILQLR+E+YTQ
Sbjct: 241 GSNPMPPEFWILPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYTQ 300

Query: 301 PYNHIKWSPTRHYCAK----------------------------------IRNRALQVAK 336
           PY+ + W   RH CAK                                  +R +ALQV  
Sbjct: 301 PYHQVNWKKVRHLCAKEDIYYPHPLIQDLLWDSLYIFTEPLLTRWPFNKLVREKALQVTM 360

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BLAST of CmoCh05G009470 vs. ExPASy Swiss-Prot
Match: A8CDT2 (Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 3.4e-114
Identity = 200/384 (52.08%), Postives = 248/384 (64.58%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHF------ 60
           MWR+ + EG  +PYL+S+NN+VGRQTW+F+PD GTPEERAEVEEARQN+Y N +      
Sbjct: 1   MWRIKIAEGGKDPYLYSTNNYVGRQTWEFDPDAGTPEERAEVEEARQNFYKNRYQVKPCG 60

Query: 61  ------KFLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                 +FL +KNF+QTIP VR++EG         I  E A+ ALRR   FF+ALQ+  G
Sbjct: 61  DLLWRLQFLGEKNFEQTIPQVRIEEGEG-------ITYEKATRALRRTVQFFSALQASDG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAE AGP F+ PPLV  +Y TGHL  +F   HRKEILRY Y HQNEDGGWGLHIEG S
Sbjct: 121 HWPAEIAGPLFFLPPLVMCVYITGHLDAVFPAEHRKEILRYIYYHQNEDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEHD---KDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWK 240
            M CT  NYI +R++GE  +    D C +ARKWI D G    IPSWGK WL ILGV +W 
Sbjct: 181 TMFCTALNYICMRIIGEGPNGGQDDACARARKWIHDHGSVTNIPSWGKTWLSILGVYDWS 240

Query: 241 GANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQ 300
           G+NPMPP+FW+L   LP  P  ++CY R+  +PMSYL+GK+FVGP+T LI QLR+E++TQ
Sbjct: 241 GSNPMPPEFWMLPSFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLIQQLREELFTQ 300

Query: 301 PYNHIKWSPTRHYCAK----------------------------------IRNRALQVAK 336
           PY+ I W  TRH CA                                   IR +AL+V  
Sbjct: 301 PYDQINWKKTRHQCAPEDLYYPHPFVQDLIWDCLYIFTEPLLTRWPLNEIIRKKALEVTM 360

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BLAST of CmoCh05G009470 vs. ExPASy Swiss-Prot
Match: O82146 (Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 7.6e-114
Identity = 197/383 (51.44%), Postives = 249/383 (65.01%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHF------ 60
           MWRL   +G N+PYL+S+NNF+GRQTW+F+PD GTP ERAEVEEAR ++++N +      
Sbjct: 1   MWRLMTAKGGNDPYLYSTNNFIGRQTWEFDPDYGTPAERAEVEEARLHFWNNRYQVKPSS 60

Query: 61  ------KFLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                 +FL++KNFKQ IP V+V++G +       I  E A+  LRRA  +F+ALQ+  G
Sbjct: 61  DVLWRMQFLKEKNFKQIIPQVKVEDGEE-------ITYEAATTTLRRAVHYFSALQADDG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAENAGP F+ PPLV  LY TGHL+ +F   HR EILRY YCHQN+DGGWGLHIEG S
Sbjct: 121 HWPAENAGPLFFLPPLVMCLYITGHLNTVFPAEHRIEILRYIYCHQNDDGGWGLHIEGHS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDE---CFKARKWILDLGGALYIPSWGKIWLCILGVCEWK 240
            M CT  +YI +R+LGE  D  E   C +ARKWILD G    IPSWGK WL ILG+ +W 
Sbjct: 181 TMFCTALSYICMRILGEGRDGGENNACARARKWILDHGSVTAIPSWGKTWLSILGLFDWS 240

Query: 241 GANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQ 300
           G+NPMPP+FW+L   LP  P  ++CY R+  +PMSYL+GK+FVGP+T LILQLR+E+Y Q
Sbjct: 241 GSNPMPPEFWILPPFLPMHPAKMWCYCRMVYMPMSYLYGKRFVGPITPLILQLREELYAQ 300

Query: 301 PYNHIKWSPTRHYCA---------------------------------KIRNRALQVAKS 336
            Y+ I W   RH CA                                 K+R +ALQ    
Sbjct: 301 AYDEINWRKVRHNCAKEDLYYPHPLIQDLMWDSLYIFTEPFLTRWPFNKLREKALQTTMK 360

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BLAST of CmoCh05G009470 vs. ExPASy TrEMBL
Match: A0A6J1KEZ8 (Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 287/376 (76.33%), Postives = 310/376 (82.45%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRL +GEG N+PYLFSSNNFVGRQTWDFEPD+GTPEERAEVEEARQNYY N FK     
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLRD+NFKQTIPIV+V+EGRD +EK+AII KETA+ ALRRAT FFAALQSHHG
Sbjct: 61  DLFWKFQFLRDRNFKQTIPIVKVEEGRDGDEKVAIINKETANNALRRATLFFAALQSHHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP FYFPPLVFSLY TGHL +IFS+HHR EILRYSYCHQN DGGWGLHI+GQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQNGDGGWGLHIQGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CMLCTVFNY+QLRLLGE+ DKDECF+ARKWILD GGA+YIPSWGKIWL ILGV +W+GAN
Sbjct: 181 CMLCTVFNYLQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYQWEGAN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGK+FVGPLT LILQLRQEIYTQ YN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQSYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           +IKWSPTRHYCAK                                 IRNRALQ AK HID
Sbjct: 301 NIKWSPTRHYCAKADKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQEAKCHID 360

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BLAST of CmoCh05G009470 vs. ExPASy TrEMBL
Match: A0A6J1KGL7 (Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 287/376 (76.33%), Postives = 310/376 (82.45%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRL +GEG N+PYLFSSNNFVGRQTWDFEPD+GTPEERAEVEEARQNYY N FK     
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLRD+NFKQTIPIV+V+EGRD +EK+AII KETA+ ALRRAT FFAALQSHHG
Sbjct: 61  DLFWKFQFLRDRNFKQTIPIVKVEEGRDGDEKVAIINKETANNALRRATLFFAALQSHHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP FYFPPLVFSLY TGHL +IFS+HHR EILRYSYCHQN DGGWGLHI+GQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQNGDGGWGLHIQGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CMLCTVFNY+QLRLLGE+ DKDECF+ARKWILD GGA+YIPSWGKIWL ILGV +W+GAN
Sbjct: 181 CMLCTVFNYLQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYQWEGAN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGK+FVGPLT LILQLRQEIYTQ YN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQSYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           +IKWSPTRHYCAK                                 IRNRALQ AK HID
Sbjct: 301 NIKWSPTRHYCAKADKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQEAKCHID 360

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BLAST of CmoCh05G009470 vs. ExPASy TrEMBL
Match: A0A6J1G4X9 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450754 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.6e-167
Identity = 289/376 (76.86%), Postives = 306/376 (81.38%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRLTVGEG ++PYLFSSNNFVGRQTWDFEPD+GTPEERAEVEEARQNYYHN FK     
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLR++NFKQTIPIVRV++         +I KETASIALRRAT+FFAALQS HG
Sbjct: 61  DLFWRFQFLRERNFKQTIPIVRVED---------VIDKETASIALRRATNFFAALQSPHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAENAGP FYFPPLVFSLY TGHL IIFSEHHRKEILRYSYCHQNEDGGWGLHI GQS
Sbjct: 121 HWPAENAGPMFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNEDGGWGLHIVGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CM CTVFNYIQLRLLGEE DKDECF+ARKWILD GGALYIPSWGKIWL ILGV EW+G N
Sbjct: 181 CMFCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLSILGVYEWEGTN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYL+GK+FVGPLT LILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           HIKWSPTRHYCAK                                 IRNRALQ+AK HID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERLYXQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHID 360

[Close]

BLAST of CmoCh05G009470 vs. ExPASy TrEMBL
Match: A0A6J1G3X4 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 6.2e-167
Identity = 285/376 (75.80%), Postives = 310/376 (82.45%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRL +GEG N+PYLFSSNNFVGRQTWDFE DEGTP++RAEVE+ARQN+Y N FK     
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                   LR++NFKQTIP+V+V+EG D +EK+A+IKKET +IALRRAT FFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP FYFPPLVFSLY TGHL IIFSE HRKEILRY+YCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CMLCTVFNYIQLRLLGEE DKDECF+ARKWILD GGA+YIPSWGKIWL ILGV EW+GAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIP++LFCYSRLTLLPMSYLFGK+FVG LT LILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           HIKWSPTRHYCAK                                 IRNRALQVAK HID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

[Close]

BLAST of CmoCh05G009470 vs. ExPASy TrEMBL
Match: A0A6J1G3U8 (Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC111450512 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 6.2e-167
Identity = 285/376 (75.80%), Postives = 310/376 (82.45%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRL +GEG N+PYLFSSNNFVGRQTWDFE DEGTP++RAEVE+ARQN+Y N FK     
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEVDEGTPKKRAEVEQARQNFYKNRFKVQCSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                   LR++NFKQTIP+V+V+EG D +EK+A+IKKET +IALRRAT FFAALQSHHG
Sbjct: 61  NFFWKFQVLRERNFKQTIPVVKVEEGIDGDEKVALIKKETVTIALRRATKFFAALQSHHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP FYFPPLVFSLY TGHL IIFSE HRKEILRY+YCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CMLCTVFNYIQLRLLGEE DKDECF+ARKWILD GGA+YIPSWGKIWL ILGV EW+GAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIP++LFCYSRLTLLPMSYLFGK+FVG LT LILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPQSLFCYSRLTLLPMSYLFGKRFVGTLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           HIKWSPTRHYCAK                                 IRNRALQVAK HID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLTWDAIQYFGEPILNSSAFKTIRNRALQVAKCHID 360

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BLAST of CmoCh05G009470 vs. NCBI nr
Match: XP_022999284.1 (beta-Amyrin Synthase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 601.7 bits (1550), Expect = 4.0e-168
Identity = 287/376 (76.33%), Postives = 310/376 (82.45%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRL +GEG N+PYLFSSNNFVGRQTWDFEPD+GTPEERAEVEEARQNYY N FK     
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLRD+NFKQTIPIV+V+EGRD +EK+AII KETA+ ALRRAT FFAALQSHHG
Sbjct: 61  DLFWKFQFLRDRNFKQTIPIVKVEEGRDGDEKVAIINKETANNALRRATLFFAALQSHHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP FYFPPLVFSLY TGHL +IFS+HHR EILRYSYCHQN DGGWGLHI+GQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQNGDGGWGLHIQGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CMLCTVFNY+QLRLLGE+ DKDECF+ARKWILD GGA+YIPSWGKIWL ILGV +W+GAN
Sbjct: 181 CMLCTVFNYLQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYQWEGAN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGK+FVGPLT LILQLRQEIYTQ YN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQSYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           +IKWSPTRHYCAK                                 IRNRALQ AK HID
Sbjct: 301 NIKWSPTRHYCAKADKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQEAKCHID 360

[Close]

BLAST of CmoCh05G009470 vs. NCBI nr
Match: XP_022999285.1 (beta-amyrin synthase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 601.7 bits (1550), Expect = 4.0e-168
Identity = 287/376 (76.33%), Postives = 310/376 (82.45%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRL +GEG N+PYLFSSNNFVGRQTWDFEPD+GTPEERAEVEEARQNYY N FK     
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYQNRFKVQCSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLRD+NFKQTIPIV+V+EGRD +EK+AII KETA+ ALRRAT FFAALQSHHG
Sbjct: 61  DLFWKFQFLRDRNFKQTIPIVKVEEGRDGDEKVAIINKETANNALRRATLFFAALQSHHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP FYFPPLVFSLY TGHL +IFS+HHR EILRYSYCHQN DGGWGLHI+GQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGVIFSQHHRNEILRYSYCHQNGDGGWGLHIQGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CMLCTVFNY+QLRLLGE+ DKDECF+ARKWILD GGA+YIPSWGKIWL ILGV +W+GAN
Sbjct: 181 CMLCTVFNYLQLRLLGEQPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYQWEGAN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGK+FVGPLT LILQLRQEIYTQ YN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQSYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           +IKWSPTRHYCAK                                 IRNRALQ AK HID
Sbjct: 301 NIKWSPTRHYCAKADKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQEAKCHID 360

[Close]

BLAST of CmoCh05G009470 vs. NCBI nr
Match: KAG6599054.1 (hypothetical protein SDJN03_08832, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 601.3 bits (1549), Expect = 5.2e-168
Identity = 290/376 (77.13%), Postives = 308/376 (81.91%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRL +GEG N+PYLFSSNNF+GRQTWDFEPDEGTPEERAEVEEARQNYYHN FK     
Sbjct: 1   MWRLKLGEGANDPYLFSSNNFMGRQTWDFEPDEGTPEERAEVEEARQNYYHNRFKVPCSS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLR++NFKQTIPIV+V+E        AII KETASIALRRAT FFAALQSHHG
Sbjct: 61  DLFWKFQFLRERNFKQTIPIVKVEE--------AIINKETASIALRRATKFFAALQSHHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAEN+GP FYFPPLVFSLY TGHL IIFSE HRKEILRY+YCHQNEDGGWGLHIEGQS
Sbjct: 121 HWPAENSGPMFYFPPLVFSLYITGHLGIIFSEEHRKEILRYAYCHQNEDGGWGLHIEGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CMLCTVFNYIQLRLLGEE DKDECF+ARKWILD GGA+YIPSWGKIWL ILGV EW+GAN
Sbjct: 181 CMLCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGAIYIPSWGKIWLSILGVYEWEGAN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGK+FVGPLT LILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKRFVGPLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCA---------------------------------KIRNRALQVAKSHID 332
           HIKW+PTRHYCA                                 KIRN+ALQ+AK HID
Sbjct: 301 HIKWNPTRHYCAKEDKCFERSLFQKLAWDSLQYLGEPILNSMAFKKIRNKALQIAKCHID 360

[Close]

BLAST of CmoCh05G009470 vs. NCBI nr
Match: KAG6599032.1 (hypothetical protein SDJN03_08810, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 597.4 bits (1539), Expect = 7.5e-167
Identity = 289/376 (76.86%), Postives = 306/376 (81.38%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRLTVGEG ++PYLFSSNNFVGRQTWDFEPD+GTPEERAEVEEARQNYYHN FK     
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLR++NFKQTIPIVRV++         +I KETASIALRRAT+FFAALQS HG
Sbjct: 61  DLFWRFQFLRERNFKQTIPIVRVED---------VIDKETASIALRRATNFFAALQSPHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAENAGP FYFPPLVFSLY TGHL IIFSEHHRKEILRYSYCHQNEDGGWGLHI GQS
Sbjct: 121 HWPAENAGPMFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNEDGGWGLHIVGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CM CTVFNYIQLRLLGEE DKDECF+ARKWILD GGALYIPSWGKIWL ILGV EW+G N
Sbjct: 181 CMFCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLSILGVYEWEGTN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYL+GK+FVGPLT LILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           HIKWSPTRHYCAK                                 IRNRALQ+AK HID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERSLFQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHID 360

[Close]

BLAST of CmoCh05G009470 vs. NCBI nr
Match: XP_022946794.1 (LOW QUALITY PROTEIN: beta-amyrin synthase-like [Cucurbita moschata])

HSP 1 Score: 597.4 bits (1539), Expect = 7.5e-167
Identity = 289/376 (76.86%), Postives = 306/376 (81.38%), Query Frame = 0


Query: 1   MWRLTVGEGENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK----- 60
           MWRLTVGEG ++PYLFSSNNFVGRQTWDFEPD+GTPEERAEVEEARQNYYHN FK     
Sbjct: 1   MWRLTVGEGADDPYLFSSNNFVGRQTWDFEPDDGTPEERAEVEEARQNYYHNRFKVPCNS 60

Query: 61  -------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHHG 120
                  FLR++NFKQTIPIVRV++         +I KETASIALRRAT+FFAALQS HG
Sbjct: 61  DLFWRFQFLRERNFKQTIPIVRVED---------VIDKETASIALRRATNFFAALQSPHG 120

Query: 121 HWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQS 180
           HWPAENAGP FYFPPLVFSLY TGHL IIFSEHHRKEILRYSYCHQNEDGGWGLHI GQS
Sbjct: 121 HWPAENAGPMFYFPPLVFSLYITGHLRIIFSEHHRKEILRYSYCHQNEDGGWGLHIVGQS 180

Query: 181 CMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGAN 240
           CM CTVFNYIQLRLLGEE DKDECF+ARKWILD GGALYIPSWGKIWL ILGV EW+G N
Sbjct: 181 CMFCTVFNYIQLRLLGEEPDKDECFRARKWILDHGGALYIPSWGKIWLSILGVYEWEGTN 240

Query: 241 PMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPYN 300
           PMPP+FWLLGKLLPFIPRTLFCYSRLTLLPMSYL+GK+FVGPLT LILQLRQEIYTQPYN
Sbjct: 241 PMPPEFWLLGKLLPFIPRTLFCYSRLTLLPMSYLYGKRFVGPLTPLILQLRQEIYTQPYN 300

Query: 301 HIKWSPTRHYCAK---------------------------------IRNRALQVAKSHID 332
           HIKWSPTRHYCAK                                 IRNRALQ+AK HID
Sbjct: 301 HIKWSPTRHYCAKEDKCFERLYXQKLAWDALQYFGEPILNSWAFKTIRNRALQIAKRHID 360

[Close]

BLAST of CmoCh05G009470 vs. TAIR 10
Match: AT1G78950.1 (Terpenoid cyclases family protein )

HSP 1 Score: 407.9 bits (1047), Expect = 7.8e-114
Identity = 202/385 (52.47%), Postives = 249/385 (64.68%), Query Frame = 0


Query: 1   MWRLTVGEGE-NNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHF----- 60
           MWRL +GEG  ++PYLF++NNF GRQTW+F+PD G+PEER  V EAR+ +Y N F     
Sbjct: 1   MWRLKIGEGNGDDPYLFTTNNFAGRQTWEFDPDGGSPEERHSVVEARRIFYDNRFHVKAS 60

Query: 61  -------KFLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHH 120
                  +FLR+K F+Q I  V+V++     EK+     ETA+ ALRR   FF+ALQ+  
Sbjct: 61  SDLLWRMQFLREKKFEQRIAPVKVED----SEKVTF---ETATSALRRGIHFFSALQASD 120

Query: 121 GHWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQ 180
           GHWPAENAGP F+ PPLVF LY TGHL  +F+  HRKEILRY YCHQ EDGGWGLHIEG 
Sbjct: 121 GHWPAENAGPLFFLPPLVFCLYITGHLDEVFTSEHRKEILRYIYCHQKEDGGWGLHIEGH 180

Query: 181 SCMLCTVFNYIQLRLLGEEHD---KDECFKARKWILDLGGALYIPSWGKIWLCILGVCEW 240
           S M CT  NYI +R+LGE  D    + C +AR+WIL  GG  YIPSWGK WL ILGV +W
Sbjct: 181 STMFCTTLNYICMRILGESPDGGHDNACGRAREWILSHGGVTYIPSWGKTWLSILGVFDW 240

Query: 241 KGANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYT 300
            G+NPMPP+FW+L    P  P  ++ Y R+  LPMSYL+GK+FVGP+TSLILQLR+E+Y 
Sbjct: 241 SGSNPMPPEFWILPSFFPVHPAKMWSYCRMVYLPMSYLYGKRFVGPITSLILQLRKELYL 300

Query: 301 QPYNHIKWSPTRHYCAK----------------------------------IRNRALQVA 336
           QPY  I W   RH CAK                                  +R +ALQ+A
Sbjct: 301 QPYEEINWMKVRHLCAKEDTYYPRPLVQELVWDSLYIFAEPFLARWPFNKLLREKALQLA 360

[Close]

BLAST of CmoCh05G009470 vs. TAIR 10
Match: AT1G78955.1 (camelliol C synthase 1 )

HSP 1 Score: 402.5 bits (1033), Expect = 3.3e-112
Identity = 197/385 (51.17%), Postives = 248/385 (64.42%), Query Frame = 0


Query: 1   MWRLTVGEG-ENNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK---- 60
           MW+L +  G +  PYLFS+NNF+GRQTW+F+PD GT EE A VEEAR+ +Y + F+    
Sbjct: 1   MWKLKIANGNKEEPYLFSTNNFLGRQTWEFDPDAGTVEELAAVEEARRKFYDDRFRVKAS 60

Query: 61  --------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHH 120
                   FL++K F+Q IP  +V++  +       I  E A+ ALR+  +F +ALQ+  
Sbjct: 61  SDLIWRMQFLKEKKFEQVIPPAKVEDANN-------ITSEIATNALRKGVNFLSALQASD 120

Query: 121 GHWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQ 180
           GHWPAENAGP F+ PPLVF LY TGHL  IF++ HR+E+LRY YCHQNEDGGWGLHIEG 
Sbjct: 121 GHWPAENAGPLFFLPPLVFCLYVTGHLHEIFTQDHRREVLRYIYCHQNEDGGWGLHIEGN 180

Query: 181 SCMLCTVFNYIQLRLLGEEHD---KDECFKARKWILDLGGALYIPSWGKIWLCILGVCEW 240
           S M CT  NYI +R+LGE  +    + C +AR WILD GGA YIPSWGK WL ILGV +W
Sbjct: 181 STMFCTTLNYICMRILGEGPNGGPGNACKRARDWILDHGGATYIPSWGKTWLSILGVFDW 240

Query: 241 KGANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYT 300
            G+NPMPP+FW+L   LP  P  ++CY RL  +PMSYL+GK+FVGP++ LILQLR+EIY 
Sbjct: 241 SGSNPMPPEFWILPSFLPIHPAKMWCYCRLVYMPMSYLYGKRFVGPISPLILQLREEIYL 300

Query: 301 QPYNHIKWSPTRHYCAK----------------------------------IRNRALQVA 336
           QPY  I W+  RH CAK                                  +R +AL VA
Sbjct: 301 QPYAKINWNRARHLCAKEDAYCPHPQIQDVIWNCLYIFTEPFLACWPFNKLLREKALGVA 360

[Close]

BLAST of CmoCh05G009470 vs. TAIR 10
Match: AT1G78960.1 (lupeol synthase 2 )

HSP 1 Score: 398.7 bits (1023), Expect = 4.7e-111
Identity = 197/385 (51.17%), Postives = 244/385 (63.38%), Query Frame = 0


Query: 1   MWRLTVGEGE-NNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHN------- 60
           MW+L +GEG   +PYLFSSNNFVGRQTW+F+P  GTPEERA VE+AR+NY  N       
Sbjct: 1   MWKLKIGEGNGEDPYLFSSNNFVGRQTWEFDPKAGTPEERAAVEDARRNYLDNRPRVKGC 60

Query: 61  -----HFKFLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHH 120
                  +FL++  F+Q IP V++ +G  +  K        A+ ALRRA SF++ALQS  
Sbjct: 61  SDLLWRMQFLKEAKFEQVIPPVKIDDGEGITYK-------NATDALRRAVSFYSALQSSD 120

Query: 121 GHWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQ 180
           GHWPAE  G  F+ PPLVF  Y TGHL  IF   HRKE+LR+ YCHQNEDGGWGLHIEG+
Sbjct: 121 GHWPAEITGTLFFLPPLVFCFYITGHLEKIFDAEHRKEMLRHIYCHQNEDGGWGLHIEGK 180

Query: 181 SCMLCTVFNYIQLRLLGEEHD---KDECFKARKWILDLGGALYIPSWGKIWLCILGVCEW 240
           S M CTV NYI LR+LGE  +    + C +AR+WILD GG  YIPSWGKIWL ILG+ +W
Sbjct: 181 SVMFCTVLNYICLRMLGEGPNGGRNNACKRARQWILDHGGVTYIPSWGKIWLSILGIYDW 240

Query: 241 KGANPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYT 300
            G NPMPP+ WLL    P       CY+R+  +PMSYL+GK+FVGPLT LI+ LR+E++ 
Sbjct: 241 SGTNPMPPEIWLLPSFFPIHLGKTLCYTRMVYMPMSYLYGKRFVGPLTPLIMLLRKELHL 300

Query: 301 QPYNHIKWSPTRHYCAK----------------------------------IRNRALQVA 336
           QPY  I W+  R  CAK                                  +R +AL+VA
Sbjct: 301 QPYEEINWNKARRLCAKEDMIYPHPLVQDLLWDTLHNFVEPILTNWPLKKLVREKALRVA 360

[Close]

BLAST of CmoCh05G009470 vs. TAIR 10
Match: AT1G78970.2 (lupeol synthase 1 )

HSP 1 Score: 386.3 bits (991), Expect = 2.4e-107
Identity = 189/382 (49.48%), Postives = 241/382 (63.09%), Query Frame = 0


Query: 1   MWRLTVGEGE-NNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK---- 60
           MW+L +G+G   +P+LFSSNNFVGRQTW F+   G+PEERA VEEAR+ +  N F+    
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 61  --------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHH 120
                   FLR+K F+Q IP ++     ++EE    I  ET + ALRR   +F ALQ+  
Sbjct: 61  SDLLWRMQFLREKKFEQGIPQLK---ATNIEE----ITYETTTNALRRGVRYFTALQASD 120

Query: 121 GHWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQ 180
           GHWP E  GP F+ PPL+F LY TGHL  +F   HRKE+LR+ YCHQNEDGGWGLHIE +
Sbjct: 121 GHWPGEITGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQNEDGGWGLHIESK 180

Query: 181 SCMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGA 240
           S M CTV NYI LR+LGE  ++D C +AR+WILD GG ++IPSWGK WL ILGV +W G 
Sbjct: 181 SVMFCTVLNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLSILGVYDWSGT 240

Query: 241 NPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPY 300
           NP PP+  +L   LP  P  + CYSR+  +PMSYL+GK+FVGP+T LIL LR+E+Y +PY
Sbjct: 241 NPTPPELLMLPSFLPIHPGKILCYSRMVSIPMSYLYGKRFVGPITPLILLLREELYLEPY 300

Query: 301 NHIKWSPTRHYCAK----------------------------------IRNRALQVAKSH 336
             I W  +R   AK                                  +R +ALQ+   H
Sbjct: 301 EEINWKKSRRLYAKEDMYYAHPLVQDLLSDTLQNFVEPLLTRWPLNKLVREKALQLTMKH 360

[Close]

BLAST of CmoCh05G009470 vs. TAIR 10
Match: AT1G78970.1 (lupeol synthase 1 )

HSP 1 Score: 386.3 bits (991), Expect = 2.4e-107
Identity = 189/382 (49.48%), Postives = 241/382 (63.09%), Query Frame = 0


Query: 1   MWRLTVGEGE-NNPYLFSSNNFVGRQTWDFEPDEGTPEERAEVEEARQNYYHNHFK---- 60
           MW+L +G+G   +P+LFSSNNFVGRQTW F+   G+PEERA VEEAR+ +  N F+    
Sbjct: 1   MWKLKIGKGNGEDPHLFSSNNFVGRQTWKFDHKAGSPEERAAVEEARRGFLDNRFRVKGC 60

Query: 61  --------FLRDKNFKQTIPIVRVKEGRDVEEKIAIIKKETASIALRRATSFFAALQSHH 120
                   FLR+K F+Q IP ++     ++EE    I  ET + ALRR   +F ALQ+  
Sbjct: 61  SDLLWRMQFLREKKFEQGIPQLK---ATNIEE----ITYETTTNALRRGVRYFTALQASD 120

Query: 121 GHWPAENAGPKFYFPPLVFSLYTTGHLSIIFSEHHRKEILRYSYCHQNEDGGWGLHIEGQ 180
           GHWP E  GP F+ PPL+F LY TGHL  +F   HRKE+LR+ YCHQNEDGGWGLHIE +
Sbjct: 121 GHWPGEITGPLFFLPPLIFCLYITGHLEEVFDAEHRKEMLRHIYCHQNEDGGWGLHIESK 180

Query: 181 SCMLCTVFNYIQLRLLGEEHDKDECFKARKWILDLGGALYIPSWGKIWLCILGVCEWKGA 240
           S M CTV NYI LR+LGE  ++D C +AR+WILD GG ++IPSWGK WL ILGV +W G 
Sbjct: 181 SVMFCTVLNYICLRMLGENPEQDACKRARQWILDRGGVIFIPSWGKFWLSILGVYDWSGT 240

Query: 241 NPMPPKFWLLGKLLPFIPRTLFCYSRLTLLPMSYLFGKQFVGPLTSLILQLRQEIYTQPY 300
           NP PP+  +L   LP  P  + CYSR+  +PMSYL+GK+FVGP+T LIL LR+E+Y +PY
Sbjct: 241 NPTPPELLMLPSFLPIHPGKILCYSRMVSIPMSYLYGKRFVGPITPLILLLREELYLEPY 300

Query: 301 NHIKWSPTRHYCAK----------------------------------IRNRALQVAKSH 336
             I W  +R   AK                                  +R +ALQ+   H
Sbjct: 301 EEINWKKSRRLYAKEDMYYAHPLVQDLLSDTLQNFVEPLLTRWPLNKLVREKALQLTMKH 360

The following BLAST results are available for this feature:

BLAST of CmoCh05G009470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
E7DN63	1.9e-117	52.74	Beta-amyrin synthase OS=Solanum lycopersicum OX=4081 GN=TTS1 PE=1 SV=1	[more]
Q2XPU7	4.3e-117	53.56	Lupeol synthase OS=Ricinus communis OX=3988 PE=1 SV=1	[more]
Q8W3Z1	7.4e-117	51.56	Beta-amyrin synthase OS=Betula platyphylla OX=78630 GN=OSCBPY PE=1 SV=1	[more]
A8CDT2	3.4e-114	52.08	Beta-amyrin synthase OS=Bruguiera gymnorhiza OX=39984 GN=BAS PE=1 SV=1	[more]
O82146	7.6e-114	51.44	Beta-amyrin synthase 2 OS=Panax ginseng OX=4054 GN=OSCPNY2 PE=2 SV=1	[more]

BLAST of CmoCh05G009470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KEZ8	1.9e-168	76.33	Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705...	[more]
A0A6J1KGL7	1.9e-168	76.33	Terpene cyclase/mutase family member OS=Cucurbita maxima OX=3661 GN=LOC111493705...	[more]
A0A6J1G4X9	3.6e-167	76.86	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114507...	[more]
A0A6J1G3X4	6.2e-167	75.80	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505...	[more]
A0A6J1G3U8	6.2e-167	75.80	Terpene cyclase/mutase family member OS=Cucurbita moschata OX=3662 GN=LOC1114505...	[more]

BLAST of CmoCh05G009470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022999284.1	4.0e-168	76.33	beta-Amyrin Synthase 2-like isoform X1 [Cucurbita maxima]	[more]
XP_022999285.1	4.0e-168	76.33	beta-amyrin synthase-like isoform X2 [Cucurbita maxima]	[more]
KAG6599054.1	5.2e-168	77.13	hypothetical protein SDJN03_08832, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6599032.1	7.5e-167	76.86	hypothetical protein SDJN03_08810, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022946794.1	7.5e-167	76.86	LOW QUALITY PROTEIN: beta-amyrin synthase-like [Cucurbita moschata]	[more]

BLAST of CmoCh05G009470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78950.1	7.8e-114	52.47	Terpenoid cyclases family protein	[more]
AT1G78955.1	3.3e-112	51.17	camelliol C synthase 1	[more]
AT1G78960.1	4.7e-111	51.17	lupeol synthase 2	[more]
AT1G78970.2	2.4e-107	49.48	lupeol synthase 1	[more]
AT1G78970.1	2.4e-107	49.48	lupeol synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.50.10.20		coord: 114..336 e-value: 5.0E-84 score: 283.8
None	No IPR available	PANTHER	PTHR11764:SF58	LUPEOL SYNTHASE 1	coord: 301..334
None	No IPR available	PANTHER	PTHR11764:SF58	LUPEOL SYNTHASE 1	coord: 1..301
IPR032697	Squalene cyclase, N-terminal	PFAM	PF13249	SQHop_cyclase_N	coord: 94..304 e-value: 8.1E-33 score: 113.8
IPR018333	Squalene cyclase	PANTHER	PTHR11764	TERPENE CYCLASE/MUTASE FAMILY MEMBER	coord: 1..301 coord: 301..334
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 116..332

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh05G009470.1	CmoCh05G009470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016104	triterpenoid biosynthetic process
cellular_component	GO:0005811	lipid droplet
molecular_function	GO:0042300	beta-amyrin synthase activity
molecular_function	GO:0000250	lanosterol synthase activity
molecular_function	GO:0016866	intramolecular transferase activity