CmoCh05G008460 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G008460
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMitotic-spindle organizing protein 1
LocationCmo_Chr05: 5323459 .. 5324562 (+)
RNA-Seq ExpressionCmoCh05G008460
SyntenyCmoCh05G008460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCAGAGGCTGCACGAACGGCTCGGGAATCGCTAGACCTTGCATTTCATATGTGTAACGTACTCGATGCCGGGATCGATCGCCACACCCTCTCTGTCCTTATTGCACTCTGTGACATGGGTGTGAACCCTGAAGCACTGGCTGCTGTTGTCAAGGAACTAAGCAGAGAACCGCCCTCATCGGAAACCATCATCTCAGGTAACAAGTCATAACCTATATGCAAAGAAACAACTCTGTTCTTCTTGTGATGATGGTTGCTAAAGTTGATATCTATGTGTTTTTAAACCACTTTACTATATATGTAGCTTACTTGGATCTTGTGAGATGAGATGGGTTGGTTGAGTTCATAATGTTTTTATGTTATGTTTTCTTGATTTCTGCAATTGGTTCGATACTAGCTACTTTCCTGCCTCAACCATTAGTCAACGAACACTTATATGCAAAGAAATGTAGAATGAGGTGAGAATAAAAATATTTAGTAAGTAGCCTACTTATAAGCTTTCATCGCATCCAATTCTTAGTAAATTTCTTTGGGTTTTTCTCGGGCCTAATTCTACTACTATCTAAGCTTTTGGGGAGGCCTTAAAGTTTTGAGTGGTTCTCATACTTGTCACAGACCTTGTCGGGTCCTGAAAGTTATTCACTAGATTCTAGTGTAGTGTTGTAGATCTATTGGACGATGCATCTCATATCAGGAGTGAACTAACTTTTTTGTTCTCATAATTAGTAAGCTTGCATTCCACAACCGTGAACTTATGATGTCCTGGGTTGAGGTGCAACCTCATCATATTTTATAGGGCAGTGTGGTGTCTTAGTCTAAACTCATCTAGAAGGTTTAACTAGTGGCCCTTCCACAGAAATTGCCATACAGTCAATGTTTTAGGGAGCGATCTACGTACCTACATGCTACTTAGTATACCCATGTTCATATAAACTAAGCAGCTCAATCCTACCATATTAACTTCAAGAAAGATGTAAAAGATATTGTTCAAATTACCCTAAGATAGTCAAGTTACATTAAATGAAGTCAAATTTCATTAAAGTTGATCTCAGGTTGCAATTAATGACACGTTAAGAAGATCAAAGTTCATTATGCAACA

mRNA sequence

ATGGATCCAGAGGCTGCACGAACGGCTCGGGAATCGCTAGACCTTGCATTTCATATGTGTAACGTACTCGATGCCGGGATCGATCGCCACACCCTCTCTGTCCTTATTGCACTCTGTGACATGGGTGTGAACCCTGAAGCACTGGCTGCTGTTGTCAAGGAACTAAGCAGAGAACCGCCCTCATCGGAAACCATCATCTCAGGTAACAAGTCATAACCTATATGCAAAGAAACAACTCTGTTCTTCTTGTGATGATGGTTGCTAAAGTTGATATCTATGTGTTTTTAAACCACTTTACTATATATGTAGCTTACTTGGATCTTGTGAGATGAGATGGGTTGGTTGAGTTCATAATGTTTTTATGTTATGTTTTCTTGATTTCTGCAATTGGTTCGATACTAGCTACTTTCCTGCCTCAACCATTAGTCAACGAACACTTATATGCAAAGAAATGTAGAATGAGGTGAGAATAAAAATATTTAGTAAGTAGCCTACTTATAAGCTTTCATCGCATCCAATTCTTAGTAAATTTCTTTGGGTTTTTCTCGGGCCTAATTCTACTACTATCTAAGCTTTTGGGGAGGCCTTAAAGTTTTGAGTGGTTCTCATACTTGTCACAGACCTTGTCGGGTCCTGAAAGTTATTCACTAGATTCTAGTGTAGTGTTGTAGATCTATTGGACGATGCATCTCATATCAGGAGTGAACTAACTTTTTTGTTCTCATAATTAGTAAGCTTGCATTCCACAACCGTGAACTTATGATGTCCTGGGTTGAGGTGCAACCTCATCATATTTTATAGGGCAGTGTGGTGTCTTAGTCTAAACTCATCTAGAAGGTTTAACTAGTGGCCCTTCCACAGAAATTGCCATACAGTCAATGTTTTAGGGAGCGATCTACGTACCTACATGCTACTTAGTATACCCATGTTCATATAAACTAAGCAGCTCAATCCTACCATATTAACTTCAAGAAAGATGTAAAAGATATTGTTCAAATTACCCTAAGATAGTCAAGTTACATTAAATGAAGTCAAATTTCATTAAAGTTGATCTCAGGTTGCAATTAATGACACGTTAAGAAGATCAAAGTTCATTATGCAACA

Coding sequence (CDS)

ATGGATCCAGAGGCTGCACGAACGGCTCGGGAATCGCTAGACCTTGCATTTCATATGTGTAACGTACTCGATGCCGGGATCGATCGCCACACCCTCTCTGTCCTTATTGCACTCTGTGACATGGGTGTGAACCCTGAAGCACTGGCTGCTGTTGTCAAGGAACTAAGCAGAGAACCGCCCTCATCGGAAACCATCATCTCAGGTAACAAGTCATAA

Protein sequence

MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPPSSETIISGNKS
Homology
[Close]
BLAST of CmoCh05G008460 vs. ExPASy Swiss-Prot
Match: Q9M0N8 (Mitotic-spindle organizing protein 1B OS=Arabidopsis thaliana OX=3702 GN=GIP1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.4e-19
Identity = 49/65 (75.38%), Postives = 54/65 (83.08%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREP- 60
          MD EA+RTARESL+L F M N+LD G+DRHTLSVLIALCD+GVNPEALA VVKEL RE  
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRESI 60

Query: 61 PSSET 65
          P S T
Sbjct: 61 PDSVT 65

[Close]
BLAST of CmoCh05G008460 vs. ExPASy Swiss-Prot
Match: Q9C9T3 (Mitotic-spindle organizing protein 1A OS=Arabidopsis thaliana OX=3702 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.4e-17
Identity = 42/66 (63.64%), Postives = 54/66 (81.82%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          M+ EAA TARESL+L F M N+L+ G+DRHTLSVLIALCD+G+NPEALA +VKEL R+  
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 SSETII 67
          ++ T +
Sbjct: 61 TTTTTV 66

[Close]
BLAST of CmoCh05G008460 vs. ExPASy Swiss-Prot
Match: A9NKD9 (Mitotic-spindle organizing protein 1 OS=Picea sitchensis OX=3332 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.6e-16
Identity = 43/58 (74.14%), Postives = 48/58 (82.76%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSRE 59
          MD  AA+ ARESLDLAF + N L  G+DRHTLS+LIALC+ GVNPEALAAVVKEL RE
Sbjct: 1  MDRTAAQNARESLDLAFSISNFLQTGLDRHTLSILIALCEHGVNPEALAAVVKELRRE 58

[Close]
BLAST of CmoCh05G008460 vs. ExPASy Swiss-Prot
Match: B5FXZ4 (Mitotic-spindle organizing protein 1 OS=Taeniopygia guttata OX=59729 GN=mzt1 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.0e-07
Identity = 22/48 (45.83%), Postives = 37/48 (77.08%), Query Frame = 0 

Query: 10 RESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSR 58
          RE++D+ F +  +L+ G+D  TLS+ + LC+ G+NPEAL++V+KEL +
Sbjct: 12 RETMDVLFEISRILNTGLDMETLSICVRLCEQGINPEALSSVIKELRK 59

[Close]
BLAST of CmoCh05G008460 vs. ExPASy Swiss-Prot
Match: Q0VFD6 (Mitotic-spindle organizing protein 1 OS=Xenopus tropicalis OX=8364 GN=mzt1 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.2e-07
Identity = 25/56 (44.64%), Postives = 41/56 (73.21%), Query Frame = 0 

Query: 10 RESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPPSSETI 66
          RE++D+   +  +L+ G+D  TLS+ + LC+ G+NPEAL++V+KEL R   +SET+
Sbjct: 12 RETMDVLLEISRLLNTGLDMETLSICVRLCEQGINPEALSSVIKELRR---ASETL 64

[Close]
BLAST of CmoCh05G008460 vs. ExPASy TrEMBL
Match: A0A6J1I8U7 (mitotic-spindle organizing protein 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111472550 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.4e-28
Identity = 69/71 (97.18%), Postives = 69/71 (97.18%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MDPEAARTARESLDLAFHM NVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60

Query: 61 SSETIISGNKS 72
          SSETI SGNKS
Sbjct: 61 SSETITSGNKS 71

[Close]
BLAST of CmoCh05G008460 vs. ExPASy TrEMBL
Match: A0A0A0LMZ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G094370 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-26
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MD EAARTARESLDLAFHM NVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIISGNKS 72
          SS+TI+S NKS
Sbjct: 61 SSDTIVSSNKS 71

[Close]
BLAST of CmoCh05G008460 vs. ExPASy TrEMBL
Match: A0A1S3B468 (mitotic-spindle organizing protein 1A-like OS=Cucumis melo OX=3656 GN=LOC103485818 PE=3 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.1e-25
Identity = 64/71 (90.14%), Postives = 66/71 (92.96%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MD EAARTARESLDLAFHM NVLD+GIDRHTLSVLIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDSGIDRHTLSVLIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIISGNKS 72
          SSET +S NKS
Sbjct: 61 SSETNVSSNKS 71

[Close]
BLAST of CmoCh05G008460 vs. ExPASy TrEMBL
Match: A0A5D3D1N9 (Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003990 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 9.4e-25
Identity = 65/72 (90.28%), Postives = 67/72 (93.06%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MD EAARTARESLDLAFHM NVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKEL +EPP
Sbjct: 1  MDQEAARTARESLDLAFHMSNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELRQEPP 60

Query: 61 SSETIIS-GNKS 72
          +SETIIS  NKS
Sbjct: 61 ASETIISASNKS 72

[Close]
BLAST of CmoCh05G008460 vs. ExPASy TrEMBL
Match: A0A6J1DE95 (mitotic-spindle organizing protein 1B OS=Momordica charantia OX=3673 GN=LOC111019635 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.6e-24
Identity = 60/67 (89.55%), Postives = 63/67 (94.03%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          MDPEAARTARESLDLAFHM N+L+ GIDRHTLS+LIALCDMGVNPEALAAVVKEL REPP
Sbjct: 1  MDPEAARTARESLDLAFHMSNILNTGIDRHTLSILIALCDMGVNPEALAAVVKELRREPP 60

Query: 61 SSETIIS 68
          S ETIIS
Sbjct: 61 SGETIIS 67

[Close]
BLAST of CmoCh05G008460 vs. TAIR 10
Match: AT4G09550.1 (AtGCP3 interacting protein 1 )

HSP 1 Score: 94.7 bits (234), Expect = 3.1e-20
Identity = 49/65 (75.38%), Postives = 54/65 (83.08%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREP- 60
          MD EA+RTARESL+L F M N+LD G+DRHTLSVLIALCD+GVNPEALA VVKEL RE  
Sbjct: 1  MDEEASRTARESLELVFRMSNILDTGLDRHTLSVLIALCDLGVNPEALATVVKELRRESI 60

Query: 61 PSSET 65
          P S T
Sbjct: 61 PDSVT 65

[Close]
BLAST of CmoCh05G008460 vs. TAIR 10
Match: AT1G73790.1 (Protein of unknown function (DUF3743) )

HSP 1 Score: 89.0 bits (219), Expect = 1.7e-18
Identity = 42/66 (63.64%), Postives = 54/66 (81.82%), Query Frame = 0 

Query: 1  MDPEAARTARESLDLAFHMCNVLDAGIDRHTLSVLIALCDMGVNPEALAAVVKELSREPP 60
          M+ EAA TARESL+L F M N+L+ G+DRHTLSVLIALCD+G+NPEALA +VKEL R+  
Sbjct: 1  MNQEAAETARESLELVFRMSNILETGLDRHTLSVLIALCDIGLNPEALATLVKELRRDSA 60

Query: 61 SSETII 67
          ++ T +
Sbjct: 61 TTTTTV 66

The following BLAST results are available for this feature:
BLAST of CmoCh05G008460 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M0N84.4e-1975.38Mitotic-spindle organizing protein 1B OS=Arabidopsis thaliana OX=3702 GN=GIP1 PE... [more]
Q9C9T32.4e-1763.64Mitotic-spindle organizing protein 1A OS=Arabidopsis thaliana OX=3702 GN=GIP2 PE... [more]
A9NKD92.6e-1674.14Mitotic-spindle organizing protein 1 OS=Picea sitchensis OX=3332 PE=3 SV=1[more]
B5FXZ41.0e-0745.83Mitotic-spindle organizing protein 1 OS=Taeniopygia guttata OX=59729 GN=mzt1 PE=... [more]
Q0VFD62.2e-0744.64Mitotic-spindle organizing protein 1 OS=Xenopus tropicalis OX=8364 GN=mzt1 PE=3 ... [more]
BLAST of CmoCh05G008460 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1I8U71.4e-2897.18mitotic-spindle organizing protein 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LMZ61.7e-2691.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G094370 PE=3 SV=1[more]
A0A1S3B4681.1e-2590.14mitotic-spindle organizing protein 1A-like OS=Cucumis melo OX=3656 GN=LOC1034858... [more]
A0A5D3D1N99.4e-2590.28Mitotic-spindle organizing protein 1A-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1DE951.6e-2489.55mitotic-spindle organizing protein 1B OS=Momordica charantia OX=3673 GN=LOC11101... [more]
BLAST of CmoCh05G008460 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G09550.13.1e-2075.38AtGCP3 interacting protein 1 [more]
AT1G73790.11.7e-1863.64Protein of unknown function (DUF3743) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022214Mitotic-spindle organizing protein 1PFAMPF12554MOZART1coord: 10..55
e-value: 3.5E-18
score: 65.1
IPR022214Mitotic-spindle organizing protein 1PANTHERPTHR28520MITOTIC-SPINDLE ORGANIZING PROTEIN 1coord: 1..63
NoneNo IPR availablePANTHERPTHR28520:SF10MITOTIC-SPINDLE ORGANIZING PROTEIN 1A-LIKEcoord: 1..63

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G008460.1CmoCh05G008460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0033566 gamma-tubulin complex localization
biological_process GO:0051415 microtubule nucleation by interphase microtubule organizing center
biological_process GO:0090307 mitotic spindle assembly
cellular_component GO:0000931 gamma-tubulin large complex
cellular_component GO:0031021 interphase microtubule organizing center
cellular_component GO:0005819 spindle