CmoCh05G004220 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G004220
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCmo_Chr05: 1943106 .. 1945407 (+)
RNA-Seq ExpressionCmoCh05G004220
SyntenyCmoCh05G004220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAATCGGCGCCGCGCAAATCAATTCAATGTGGCCGTCGTTTCTCAGCCTCGTATCCTTGTACGGCGGCTATCTCCGCCGCTGCTTCACCGCCGCGGGCCTTACGCAGCAAGCCATCGACATCGACGCCGAAACGACTCTTTCCTTTTGGGGCCCTAAGCCCAGTAAATCCAACGCGAAACCCTCCCTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCCACAAGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTTCCCGACCTCGTCTTCTTCGGCGATTCGACGACCAAATCGGCGGAGAGGACTGAGGTTTTTCAGGCGATCTCGATCGGAAAGCTTCTGGAGAAGATCGGAGTGAAGAAATACTCGGTGATGGGAACGAGCTACGGCGGATTTGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAAGTTGTAATCGCGAGCTCCGGCGTTAATATGAGACGGAAAGATAACGATGCGATGTTGAAAAGAGCTAACGTGGAGAATATCGATGAATTTTTGCTGCCGATGACGGCGGAACAGTTACGGACGTTAATGAAGCTTGCTGTATTCAAGGGAGTGGGCAGACGGATGCCGGACTTCTTCTTGAACGATTTCATCCATGTAAGGACAAAATTCGTTTATATTATTTTATTATTATTTATTTATTTTCTTTTAAATATTTCTTTAATCTATTTATTTGTTTATTCTTTTTCTGTCATAAAAATATTATTAAAATTTAATAAGATATTTTATATATTTAAATAAAAAAAACGAAAGGATTAATTATATTTATCAATTATTCGGATAAATAATTATATAAATATAATTTATGATTTGGCTAGAATGTTTTCGTAACGTAATGGAATTACGAAGATATCGTGAACGCTTGGAATAACATACCAGTCAGCCAATTTAGTTTATCTTTTTAATCACGAGAAATCATAGTAATTTTATATTTCAAGATATTATTTAAAAGTGAGACGGTGGTAAATAATTGTTGGTAAGAAAAACCATCCCATTATTTTTTCAATAATTTAAAGCTTTATTATTTATTAGGTGACGTCTGGTTTGACCTTTAAAAAAAGACAAAATAAACAAAAAATAAATAAATATAAATATTGCAAATTGTGTATGGTGTTAAATTGTTTTGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTGAAATTTATTTATTTATTTTTTCCATATGATATATTTATAAATTCAGAAAATTAGTGTATGAAATGAATTAATTAATAATTTGTTTAAATAAATCCAGAAATTGTACATGGAAAAGAGGAAGGAAAAGATCGAACTACTCAAGTCGCTCACGTTTGGGCGAGAAGATACCATCGACCTCTCTCCACTCTTTCAGGTAGGTTGAAGGTTAGCTCGTTCGAGAGTGTCGTATTTTTATTAAAATTAACAATAAATTTGAAGTGGTTTATGTTCGGGATGGATATGCAGGAGGTATTGATAATATGGGGAGATCATGATCAGTTATTTCCCTTGGAAATGGCTAAGGAGCTGAAGGAGTGAGTTTATTTTATTTTTATTTTTATTTTTTTTATTTTAAATATTGGTGGGCTTAGATTATAAACGTGTGGTGTAGGATTCTCGGGGAAAAGACAAGGCTGGAGGTGATGAAGGAGACCTCGCACGTGCCGCAGATTGAGGCCCCTGCAGAATTCAACCGTGTGGTCAAGAGTTTCTTGTGTGGACTCTGATTTAATTATTTCC

mRNA sequence

ATAATCGGCGCCGCGCAAATCAATTCAATGTGGCCGTCGTTTCTCAGCCTCGTATCCTTGTACGGCGGCTATCTCCGCCGCTGCTTCACCGCCGCGGGCCTTACGCAGCAAGCCATCGACATCGACGCCGAAACGACTCTTTCCTTTTGGGGCCCTAAGCCCAGTAAATCCAACGCGAAACCCTCCCTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCCACAAGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTTCCCGACCTCGTCTTCTTCGGCGATTCGACGACCAAATCGGCGGAGAGGACTGAGGTTTTTCAGGCGATCTCGATCGGAAAGCTTCTGGAGAAGATCGGAGTGAAGAAATACTCGGTGATGGGAACGAGCTACGGCGGATTTGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAAGTTGTAATCGCGAGCTCCGGCGTTAATATGAGACGGAAAGATAACGATGCGATGTTGAAAAGAGCTAACGTGGAGAATATCGATGAATTTTTGCTGCCGATGACGGCGGAACAGTTACGGACGTTAATGAAGCTTGCTGTATTCAAGGGAGTGGGCAGACGGATGCCGGACTTCTTCTTGAACGATTTCATCCATAAATTGTACATGGAAAAGAGGAAGGAAAAGATCGAACTACTCAAGTCGCTCACGTTTGGGCGAGAAGATACCATCGACCTCTCTCCACTCTTTCAGGAGGTATTGATAATATGGGGAGATCATGATCAGTTATTTCCCTTGGAAATGGCTAAGGAGCTGAAGGAGATTCTCGGGGAAAAGACAAGGCTGGAGGTGATGAAGGAGACCTCGCACGTGCCGCAGATTGAGGCCCCTGCAGAATTCAACCGTGTGGTCAAGAGTTTCTTGTGTGGACTCTGATTTAATTATTTCC

Coding sequence (CDS)

ATGTGGCCGTCGTTTCTCAGCCTCGTATCCTTGTACGGCGGCTATCTCCGCCGCTGCTTCACCGCCGCGGGCCTTACGCAGCAAGCCATCGACATCGACGCCGAAACGACTCTTTCCTTTTGGGGCCCTAAGCCCAGTAAATCCAACGCGAAACCCTCCCTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCCACAAGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTTCCCGACCTCGTCTTCTTCGGCGATTCGACGACCAAATCGGCGGAGAGGACTGAGGTTTTTCAGGCGATCTCGATCGGAAAGCTTCTGGAGAAGATCGGAGTGAAGAAATACTCGGTGATGGGAACGAGCTACGGCGGATTTGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAAGTTGTAATCGCGAGCTCCGGCGTTAATATGAGACGGAAAGATAACGATGCGATGTTGAAAAGAGCTAACGTGGAGAATATCGATGAATTTTTGCTGCCGATGACGGCGGAACAGTTACGGACGTTAATGAAGCTTGCTGTATTCAAGGGAGTGGGCAGACGGATGCCGGACTTCTTCTTGAACGATTTCATCCATAAATTGTACATGGAAAAGAGGAAGGAAAAGATCGAACTACTCAAGTCGCTCACGTTTGGGCGAGAAGATACCATCGACCTCTCTCCACTCTTTCAGGAGGTATTGATAATATGGGGAGATCATGATCAGTTATTTCCCTTGGAAATGGCTAAGGAGCTGAAGGAGATTCTCGGGGAAAAGACAAGGCTGGAGGTGATGAAGGAGACCTCGCACGTGCCGCAGATTGAGGCCCCTGCAGAATTCAACCGTGTGGTCAAGAGTTTCTTGTGTGGACTCTGA

Protein sequence

MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLCGL
Homology
BLAST of CmoCh05G004220 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 3.2e-10
Identity = 62/266 (23.31%), Postives = 116/266 (43.61%), Query Frame = 0

Query: 51  KPSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAI--SI 110
           KP +LLLHGF P   + WR Q++    ++ V   DL  +G+S   + + +     +   I
Sbjct: 91  KPLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDLRGYGESDAPAHQESYKLDCLIADI 150

Query: 111 GKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIAS----SGVNMRRKDNDAML 170
             +L+ +G  K  ++G  +GG +A+ +A  +PE I K+++ +    S        + A L
Sbjct: 151 KDILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIVINFPHPSVFTEYILRHPAQL 210

Query: 171 KRAN------VENIDEFLLPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRK 230
            R++      +    EF+  +   +    +  +   G+GR+       D    +Y+  + 
Sbjct: 211 FRSSFYYFFQIPRFPEFMFSINDFKALKHLFTSQSTGIGRKGRQLTTEDLEAYVYVFSQP 270

Query: 231 EKIELLKSLTFGREDTIDLSPLFQEV-----LIIWGDHDQLFPLEMAKELKEILGEKTRL 290
                L        +     PL   +     L++WG+ D    +EMA+  K  +    RL
Sbjct: 271 ---GALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGEEDAFMEVEMAEVTKIYVKNYFRL 330

Query: 291 EVMKETSHVPQIEAPAEFNRVVKSFL 300
            ++ E SH  Q + P   N ++ +FL
Sbjct: 331 TILSEGSHWLQQDQPDIVNGLIWAFL 352

BLAST of CmoCh05G004220 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.6e-09
Identity = 62/272 (22.79%), Postives = 118/272 (43.38%), Query Frame = 0

Query: 51  KPSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAI--SI 110
           KP +LLLHGF P   + WR Q++    ++ V   DL  +G++      +      +   I
Sbjct: 93  KPLMLLLHGF-PEFWYSWRYQLREFKSEYRVVALDLRGYGETDAPIHRQNYKLDCLITDI 152

Query: 111 GKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKD--------N 170
             +L+ +G  K  ++G  +GG +A+ +A  +PE + K+++    +N    +        +
Sbjct: 153 KDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIV----INFPHPNVFTEYILRH 212

Query: 171 DAMLKRANVENIDEFLLPMTAEQLRTLMKLAVFK--------GVGRRMPDFFLNDFIHKL 230
            A L +++      F +P   E + ++    V K        G+GR+       D    +
Sbjct: 213 PAQLLKSSYYYF--FQIPWFPEFMFSINDFKVLKHLFTSHSTGIGRKGCQLTTEDLEAYI 272

Query: 231 YMEKRKEKIELLKSLTFGREDTIDLSPLFQEV-----LIIWGDHDQLFPLEMAKELKEIL 290
           Y+  +      L        +     PL   +     L++WG++D    +EMA+  K  +
Sbjct: 273 YVFSQP---GALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGENDAFMEVEMAEVTKIYV 332

Query: 291 GEKTRLEVMKETSHVPQIEAPAEFNRVVKSFL 300
               RL ++ E SH  Q + P   N+++ +FL
Sbjct: 333 KNYFRLTILSEASHWLQQDQPDIVNKLIWTFL 354

BLAST of CmoCh05G004220 vs. ExPASy Swiss-Prot
Match: Q49KF8 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase 1 OS=Pseudomonas putida OX=303 GN=mhpC1 PE=3 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 3.5e-09
Identity = 67/266 (25.19%), Postives = 118/266 (44.36%), Query Frame = 0

Query: 51  KPSLLLLHGFGPMAI-W-QWRPQVQFLAD-DFDVYVPDLVFF--GDSTTKSAERTEVFQA 110
           K ++++LHG GP A  W  +   ++ L +  F V + D   +   DS   +  R+++   
Sbjct: 33  KETVVMLHGSGPGASGWANFNRNIEPLVNAGFRVILMDCPGWSKSDSIVSTGSRSDLNAR 92

Query: 111 ISIGKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLK 170
           +  G L++K+ + K  ++G S GG  A   +  WPER+ K+V+   G           + 
Sbjct: 93  VLKG-LVDKLDLDKIHILGNSMGGHTAVAFSLTWPERVGKLVLMGGGTG--GVSPFVPMP 152

Query: 171 RANVENIDEFLLPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLY------MEKRKE 230
              ++ ++      T E L+ +M + V+            +D   +L+      M  RK+
Sbjct: 153 SEGIKLLNGLYREPTIENLKKMMSIFVYD----------TSDLTEELFQTRLDNMLARKD 212

Query: 231 KIE-LLKSLTFGREDTID----LSPLFQEVLIIWGDHDQLFPLEMAKELKEILG-EKTRL 290
            +E    SL    +   D    L  +  E L+IWG +D+  PL+    L+ + G   ++L
Sbjct: 213 HLENFTASLAANLKQFPDFGHRLGEINAETLVIWGRNDRFVPLDTG--LRLVAGISNSQL 272

Query: 291 EVMKETSHVPQIEAPAEFNRVVKSFL 300
            V  +  H  Q E    FNR+V  FL
Sbjct: 273 HVFNKCGHWAQWEHADTFNRMVLDFL 283

BLAST of CmoCh05G004220 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.8e-09
Identity = 65/266 (24.44%), Postives = 118/266 (44.36%), Query Frame = 0

Query: 52  PSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKL 111
           P++LL+HG G  +   W      LA  F V  PDL+  G S    A+ +    A  +  L
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 112 LEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVEN 171
           L  + +++ +++G S GG VA   A  +P+ ++++++ S+G     KD + + + A++  
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAG--GVTKDVNIVFRLASLPM 157

Query: 172 IDEFL----LPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSL 231
             E +    LP+    ++   ++ V K +G       L + +  L      +  E   S 
Sbjct: 158 GSEAMALLRLPLVLPAVQIAGRI-VGKAIGTTSLGHDLPNVLRIL-----DDLPEPTASA 217

Query: 232 TFGRE------------DTIDLSPLFQ--EVLIIWGDHDQLFPLEMAKELKEILGEKTRL 291
            FGR               +D   L +   V IIWG  D + P+  A  +       ++L
Sbjct: 218 AFGRTLRAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDVVLPVRHA-HMAHAAMPGSQL 277

Query: 292 EVMKETSHVPQIEAPAEFNRVVKSFL 300
           E+ + + H P  + PA F  +V+ F+
Sbjct: 278 EIFEGSGHFPFHDDPARFIDIVERFM 293

BLAST of CmoCh05G004220 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.8e-09
Identity = 65/266 (24.44%), Postives = 118/266 (44.36%), Query Frame = 0

Query: 52  PSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKL 111
           P++LL+HG G  +   W      LA  F V  PDL+  G S    A+ +    A  +  L
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 112 LEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVEN 171
           L  + +++ +++G S GG VA   A  +P+ ++++++ S+G     KD + + + A++  
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAG--GVTKDVNIVFRLASLPM 157

Query: 172 IDEFL----LPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSL 231
             E +    LP+    ++   ++ V K +G       L + +  L      +  E   S 
Sbjct: 158 GSEAMALLRLPLVLPAVQIAGRI-VGKAIGTTSLGHDLPNVLRIL-----DDLPEPTASA 217

Query: 232 TFGRE------------DTIDLSPLFQ--EVLIIWGDHDQLFPLEMAKELKEILGEKTRL 291
            FGR               +D   L +   V IIWG  D + P+  A  +       ++L
Sbjct: 218 AFGRTLRAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDVVLPVRHA-HMAHAAMPGSQL 277

Query: 292 EVMKETSHVPQIEAPAEFNRVVKSFL 300
           E+ + + H P  + PA F  +V+ F+
Sbjct: 278 EIFEGSGHFPFHDDPARFIDIVERFM 293

BLAST of CmoCh05G004220 vs. ExPASy TrEMBL
Match: A0A6J1HE07 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462697 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.4e-170
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF
Sbjct: 28  MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 87

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 88  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 147

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 148 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 207

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL
Sbjct: 208 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 267

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC
Sbjct: 268 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 327

Query: 301 GL 303
           GL
Sbjct: 328 GL 329

BLAST of CmoCh05G004220 vs. ExPASy TrEMBL
Match: A0A6J1KA03 (uncharacterized protein LOC111492447 OS=Cucurbita maxima OX=3661 GN=LOC111492447 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.2e-166
Identity = 295/302 (97.68%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFG S TKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSMTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLT GREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVP IE+PAEFNRVVK FLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPHIESPAEFNRVVKRFLC 300

Query: 301 GL 303
           G+
Sbjct: 301 GI 302

BLAST of CmoCh05G004220 vs. ExPASy TrEMBL
Match: A0A5D3BNV7 (Monoacylglycerol lipase ABHD6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00070 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.6e-150
Identity = 263/301 (87.38%), Postives = 284/301 (94.35%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLYG YLRRCF+AAGL+QQ I ID ETT+SFWGPKP KS AKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGRYLRRCFSAAGLSQQVIHIDDETTISFWGPKPHKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GP AIWQWR QVQFLADDFD+YVPDLVFFG STTKSAERTE+FQA+SIGKL+E IGVKKY
Sbjct: 61  GPAAIWQWRQQVQFLADDFDLYVPDLVFFGGSTTKSAERTEIFQAMSIGKLVEMIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SV+GTSYGGFVAYHMARIWPERIEKV+IASSGVNMRRKDN+AMLKRANVE IDEFLLP+T
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKG GR+MPDFF NDFIHKLYME R++KIELLKSLT GRED+I+LSPL
Sbjct: 181 AEQLRTLMKLAVFKGGGRKMPDFFFNDFIHKLYMENREQKIELLKSLTLGREDSINLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
            QEVLIIWGDHDQLFPLEMAKELK I+GEKTRLEV+KETSHVPQIEAPA+FN++VKSFLC
Sbjct: 241 SQEVLIIWGDHDQLFPLEMAKELKGIIGEKTRLEVLKETSHVPQIEAPAQFNQLVKSFLC 300

Query: 301 G 302
           G
Sbjct: 301 G 301

BLAST of CmoCh05G004220 vs. ExPASy TrEMBL
Match: A0A1S3BDU7 (monoacylglycerol lipase ABHD6-like OS=Cucumis melo OX=3656 GN=LOC103488527 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.6e-150
Identity = 263/301 (87.38%), Postives = 284/301 (94.35%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLYG YLRRCF+AAGL+QQ I ID ETT+SFWGPKP KS AKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGRYLRRCFSAAGLSQQVIHIDDETTISFWGPKPHKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GP AIWQWR QVQFLADDFD+YVPDLVFFG STTKSAERTE+FQA+SIGKL+E IGVKKY
Sbjct: 61  GPAAIWQWRQQVQFLADDFDLYVPDLVFFGGSTTKSAERTEIFQAMSIGKLVEMIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SV+GTSYGGFVAYHMARIWPERIEKV+IASSGVNMRRKDN+AMLKRANVE IDEFLLP+T
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKG GR+MPDFF NDFIHKLYME R++KIELLKSLT GRED+I+LSPL
Sbjct: 181 AEQLRTLMKLAVFKGGGRKMPDFFFNDFIHKLYMENREQKIELLKSLTLGREDSINLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
            QEVLIIWGDHDQLFPLEMAKELK I+GEKTRLEV+KETSHVPQIEAPA+FN++VKSFLC
Sbjct: 241 SQEVLIIWGDHDQLFPLEMAKELKGIIGEKTRLEVLKETSHVPQIEAPAQFNQLVKSFLC 300

Query: 301 G 302
           G
Sbjct: 301 G 301

BLAST of CmoCh05G004220 vs. ExPASy TrEMBL
Match: A0A6J1BQG4 (uncharacterized protein LOC111004859 OS=Momordica charantia OX=3673 GN=LOC111004859 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.9e-149
Identity = 264/303 (87.13%), Postives = 285/303 (94.06%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPK--PSKSNAKPSLLLLH 60
           MWPSFLSLVSLYGGYLRRCFTAAGL+ QA++ID ETT++FWGPK   +K   K SL+LLH
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLSGQAVEIDNETTIAFWGPKIITNKPTGKRSLVLLH 60

Query: 61  GFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVK 120
           GFGPMAIWQWR QVQFLADDFDVYVPDLVFFG STTKS+ERTEVFQAIS+GKLLEKIGVK
Sbjct: 61  GFGPMAIWQWRKQVQFLADDFDVYVPDLVFFGGSTTKSSERTEVFQAISVGKLLEKIGVK 120

Query: 121 KYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLP 180
           KYSVMGTSYGGFVAYHMAR WPERIEKV+IASSGVNMRRKDN+AMLKRANVE ID+FLLP
Sbjct: 121 KYSVMGTSYGGFVAYHMARFWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDDFLLP 180

Query: 181 MTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLS 240
           +TAEQLRTLMKLAVFK VGRR+PDFFLNDFIHKLYME R+EKIELLKSLT GREDT++LS
Sbjct: 181 VTAEQLRTLMKLAVFKEVGRRLPDFFLNDFIHKLYMENREEKIELLKSLTIGREDTLNLS 240

Query: 241 PLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSF 300
           PL +EVLIIWGDHDQLFPL+MAKELKEILGEKTRLEVMKETSHVPQIE+PAEFNR+VKSF
Sbjct: 241 PLSEEVLIIWGDHDQLFPLQMAKELKEILGEKTRLEVMKETSHVPQIESPAEFNRLVKSF 300

Query: 301 LCG 302
           L G
Sbjct: 301 LNG 303

BLAST of CmoCh05G004220 vs. NCBI nr
Match: XP_022962163.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 608.6 bits (1568), Expect = 2.9e-170
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF
Sbjct: 28  MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 87

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 88  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 147

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 148 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 207

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL
Sbjct: 208 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 267

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC
Sbjct: 268 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 327

Query: 301 GL 303
           GL
Sbjct: 328 GL 329

BLAST of CmoCh05G004220 vs. NCBI nr
Match: KAG6598595.1 (Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 602.4 bits (1552), Expect = 2.1e-168
Identity = 298/302 (98.68%), Postives = 301/302 (99.67%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSF+SLVSLYGGYLRRCFT+AGLTQQAI+IDAETTLSFWGPKPSKSNAKPSLLLLHGF
Sbjct: 1   MWPSFVSLVSLYGGYLRRCFTSAGLTQQAIEIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLT GREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300

Query: 301 GL 303
           GL
Sbjct: 301 GL 302

BLAST of CmoCh05G004220 vs. NCBI nr
Match: XP_023546569.1 (epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.5 bits (1534), Expect = 2.6e-166
Identity = 297/302 (98.34%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLY GYLRRCFTAAGLTQQAIDIDAETTLSFWGPK SKSNAKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFG STTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLT GREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRV KSFLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300

Query: 301 GL 303
           GL
Sbjct: 301 GL 302

BLAST of CmoCh05G004220 vs. NCBI nr
Match: XP_022997555.1 (uncharacterized protein LOC111492447 [Cucurbita maxima])

HSP 1 Score: 595.5 bits (1534), Expect = 2.6e-166
Identity = 295/302 (97.68%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFG S TKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSMTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLT GREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVP IE+PAEFNRVVK FLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPHIESPAEFNRVVKRFLC 300

Query: 301 GL 303
           G+
Sbjct: 301 GI 302

BLAST of CmoCh05G004220 vs. NCBI nr
Match: XP_038885739.1 (monoacylglycerol lipase ABHD6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 563.9 bits (1452), Expect = 8.2e-157
Identity = 272/301 (90.37%), Postives = 291/301 (96.68%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60
           MWPSFLSLVS YGGYLRRCF+AAGL+ Q I+ID +TT++FWGPKP+KS AKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSFYGGYLRRCFSAAGLSHQVINIDDQTTIAFWGPKPNKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFG STTKSAERTE+FQA+S+GKL+EKIGVKKY
Sbjct: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSAERTEIFQAMSVGKLMEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SV+GTSYGGFVAYHMARIWPERIEKV+IASSGVNMRRKDNDAMLKRANVE IDEFLLP+T
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNDAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKR+EKIELLKSLT GREDTI LSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKREEKIELLKSLTLGREDTISLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEI+GEKTRLEVMK+TSHVPQIEAPAEFNR+VK+FLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEIIGEKTRLEVMKDTSHVPQIEAPAEFNRLVKTFLC 300

Query: 301 G 302
           G
Sbjct: 301 G 301

BLAST of CmoCh05G004220 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 358.6 bits (919), Expect = 4.9e-99
Identity = 177/302 (58.61%), Postives = 224/302 (74.17%), Query Frame = 0

Query: 4   SFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKP---SKSNAKPSLLLLHGF 63
           S+LS   L  GYLRRC  AAGLT Q + ID+ETT+ FWGP P      + +P +LLLHGF
Sbjct: 5   SYLSPTRLLEGYLRRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGF 64

Query: 64  GPMAIWQWRPQVQ-FLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKK 123
           GP ++WQWR Q+Q F    F VY PDLVFFGDST+ S  RTEVFQA  + KL+ KIG+ K
Sbjct: 65  GPSSMWQWRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGK 124

Query: 124 YSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPM 183
           Y+V GTSYGGFVAYHMA++WPE++EKVVIASSG+NMR+ D +++L+R+N E I++ +LP 
Sbjct: 125 YNVAGTSYGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGESLLQRSNCECIEKVMLPS 184

Query: 184 TAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSP 243
           TA + RTLM LA    + R  PD   ND I+ LY + RKEKIELLK +TFGR + +++  
Sbjct: 185 TATEFRTLMALASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDS 244

Query: 244 LFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFL 302
           L QEVLI+WGD DQ+FP++MA ELKEILG+KT+LE++  TSHVPQIE   EFN +V  FL
Sbjct: 245 LSQEVLIVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

BLAST of CmoCh05G004220 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.2e-91
Identity = 163/302 (53.97%), Postives = 224/302 (74.17%), Query Frame = 0

Query: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSN---AKPSLLLL 60
           M PSFLS+       LRR F++AGL+ Q + ID+ETT+ FWGP PS S+    KPSLLLL
Sbjct: 1   MAPSFLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLL 60

Query: 61  HGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGV 120
           HGFGP A+WQW  QV+ L+  F +YVPDLVFFG S++    R+E+FQA+ +GKL+EK+ V
Sbjct: 61  HGFGPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEV 120

Query: 121 KKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLL 180
           +++SV+GTSYGGFVAY+MA+++PE++EKVV+ASSGVN+RR DN+A + RA    I E +L
Sbjct: 121 ERFSVVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVML 180

Query: 181 PMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDL 240
           P +A  LR    +   K +   +PDF LNDF  K+Y EKR+EK ELL+ L+ G++D  ++
Sbjct: 181 PASATDLRRFSGMVSSKRLD-YVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNV 240

Query: 241 SPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKS 300
           SP+ Q+V++IWG+ DQ+FPL+MA +LKE+LG K  L+V+++TSH+PQ E   EFN  V S
Sbjct: 241 SPIQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMS 300

BLAST of CmoCh05G004220 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.3e-55
Identity = 116/284 (40.85%), Postives = 180/284 (63.38%), Query Frame = 0

Query: 20  FTAAGLTQQAIDIDAETTLSFWGPKPS-KSNAKPSLLLLHGFGPMAIWQWRPQVQFLADD 79
           F  +GL    ID+   T ++FW  K   +S  KP+LLL+HG G  AIWQW    + L+  
Sbjct: 19  FKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIHGLGATAIWQWYDVARRLSRY 78

Query: 80  FDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKYSVMGTSYGGFVAYHMARI 139
           F++Y+PDLVFFG S+T   ER+++FQA ++ + LE   VKK+S++G SYGGFV Y MA +
Sbjct: 79  FNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKFSLVGLSYGGFVGYRMASM 138

Query: 140 WPERIEKVVIASSGVNMRRKDNDA-MLKRANVENIDEFLLPMTAEQLRTLMKLAVFK-GV 199
           + + +EKVVI  + V +  KD  A + K ++++   + L+P + ++LR LM    +K  +
Sbjct: 139 YADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRELMGYIFYKPAL 198

Query: 200 GRRMPDFFLNDFI-HKLYMEKRKEKIELLKSLTFGREDTIDLSPLFQEVLIIWGDHDQLF 259
            R +P   L+DFI H L  +  +EK EL+K++   R  + ++  L Q  LIIWG+HDQ+F
Sbjct: 199 ARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIIS-EIPKLKQPTLIIWGEHDQVF 258

Query: 260 PLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFL 300
           PLEM K L++ +G+  +L ++K T H+   E P +F +++KSFL
Sbjct: 259 PLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSFL 301

BLAST of CmoCh05G004220 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.2e-54
Identity = 118/289 (40.83%), Postives = 174/289 (60.21%), Query Frame = 0

Query: 17  RRCFTAAGLTQQAIDID-----AETTLSFWGPKPSKSNAKPSLLLLHGFGPMAIWQWRPQ 76
           R+ F  AGL     D+      A T +  W PK S + +KP+LLLLHGFG  A+WQ+   
Sbjct: 22  RQSFANAGLRSVTTDLSHGNSIASTAMHCWIPK-SPNRSKPNLLLLHGFGANAMWQYGEH 81

Query: 77  VQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKYSVMGTSYGGFV 136
           ++     F+VYVPDL+FFG S+T    RTE FQA  + +L+E  GV++ +++G SYGGFV
Sbjct: 82  LRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMRLMEAHGVQRMNIVGISYGGFV 141

Query: 137 AYHMARIWPERIEKVVIASSGVNMRRKD-NDAMLKRANVENIDEFLLPMTAEQLRTLMKL 196
            Y +A  +PE +EK+V+  +GV +  KD  D + K  N+E     L+P T E+L+ L++ 
Sbjct: 142 GYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLEEATGILIPQTPEKLKELIRF 201

Query: 197 AVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPLFQEVLIIWGD 256
           +  K + + +P FFL DFI  +  E  +EK +L+KS+   R    DL  + Q+ LIIWG+
Sbjct: 202 SFVKPI-KGVPSFFLWDFIDVMCTEFVEEKRDLIKSILKDRR-LSDLPRIKQKSLIIWGE 261

Query: 257 HDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFL 300
            DQ+FPLE+   LK  +GE   + V+K+  H   +E   EF + +KSFL
Sbjct: 262 EDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVKHLKSFL 307

BLAST of CmoCh05G004220 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-50
Identity = 116/299 (38.80%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 4   SFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGFGPM 63
           S  S VS      +  F+ AGL     D+   T    W P  +  + KP+LLLLHG G  
Sbjct: 3   SCFSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPL-THIHTKPTLLLLHGIGAN 62

Query: 64  AIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKYSVM 123
           A+WQW   +      F+VYVPDL+FFGDS T   +R+E FQA  + K ++  GV+  +V 
Sbjct: 63  AMWQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTMTVA 122

Query: 124 GTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDN-DAMLKRANVENIDEFLLPMTAE 183
           G SYGGFVAY +A  + ER+++VV+  +GV +  KD+ D M K  + E     L P +  
Sbjct: 123 GLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSPS 182

Query: 184 QLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPLFQ 243
            LR L++L+ +K     +P  F  D+IH +  +  +E+ EL+++L  GR    +L  + Q
Sbjct: 183 MLRRLLQLSFYK-PPIWIPSCFAMDYIHVMCKDYLQERKELVEALHKGRR-FANLPKITQ 242

Query: 244 EVLIIWGDHDQLFPLEMAKELKEILGE-KTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 301
             L+IWG+ DQ+FP+E+A  LK  LGE + +L ++K+T H    E P E  + +KSFLC
Sbjct: 243 PTLMIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINEEKPKEMYKHMKSFLC 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6IE263.2e-1023.31Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2[more]
Q8IUS51.6e-0922.79Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2[more]
Q49KF83.5e-0925.192-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase 1 OS=Ps... [more]
P9WNH27.8e-0924.44Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ... [more]
P9WNH37.8e-0924.44Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HE071.4e-170100.00epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462697 PE=4 SV=1[more]
A0A6J1KA031.2e-16697.68uncharacterized protein LOC111492447 OS=Cucurbita maxima OX=3661 GN=LOC111492447... [more]
A0A5D3BNV73.6e-15087.38Monoacylglycerol lipase ABHD6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BDU73.6e-15087.38monoacylglycerol lipase ABHD6-like OS=Cucumis melo OX=3656 GN=LOC103488527 PE=4 ... [more]
A0A6J1BQG48.9e-14987.13uncharacterized protein LOC111004859 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
Match NameE-valueIdentityDescription
XP_022962163.12.9e-170100.00epoxide hydrolase 4-like [Cucurbita moschata][more]
KAG6598595.12.1e-16898.68Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023546569.12.6e-16698.34epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo][more]
XP_022997555.12.6e-16697.68uncharacterized protein LOC111492447 [Cucurbita maxima][more]
XP_038885739.18.2e-15790.37monoacylglycerol lipase ABHD6-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G33180.14.9e-9958.61alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.12.2e-9153.97alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.13.3e-5540.85alpha/beta-Hydrolases superfamily protein [more]
AT5G09430.12.2e-5440.83alpha/beta-Hydrolases superfamily protein [more]
AT4G39955.11.4e-5038.80alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 136..149
score: 32.51
coord: 244..258
score: 42.99
coord: 122..135
score: 34.48
coord: 78..93
score: 35.13
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 54..293
e-value: 2.8E-14
score: 54.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 16..300
e-value: 8.0E-45
score: 155.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 44..299
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 5..300
NoneNo IPR availablePANTHERPTHR43139:SF52ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G004220.1CmoCh05G004220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity