CmoCh05G001710 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh05G001710
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRING/U-box superfamily protein
LocationCmo_Chr05: 738564 .. 742311 (+)
RNA-Seq ExpressionCmoCh05G001710
SyntenyCmoCh05G001710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGGGCAAAGGAGTAGTGTCGGTTCATTGTCAGAAACAATTAACTTTGAACATGGATCTTCATCAAATAATCCTGGTAACCAACCAGTTTACTGGAATAACATGTGGAATCCAGCAGAAAACCGAATACCAGATTATTTATTGCCTACTTCTGATGTAAATTCTGGATATGTAAGCTCTGTAAGCCATGAACAACGAAGTTTGAGCAGATGGAGCTTGGGTGAACCTAGCTCTTGTGATATGCAAACTGATGTGCTGCCTGATGAGCAGAAAGCTGAGCTTGGATGGTCTTCAATGACTCGTGATGCAGATGGTCCCTTCACCGAAAACCAACTCTGTGAGCCATCAAATAATCCTTCACTGGGTCATGTGAATTTGAGCCCACTGATTATACAAAATTCTAATTCTAATAGTATTCCTCATACTCTGAATTTAAATGCAAGTTTTGCTAGTCATGGAGGTGACAGTAGCAGAGTTAACGAAGGTACTAACGTGTATAAATCGAGTGGATCAGAAGAAGGGGGGATTTTATGTTCTAGTGCATCTGATCCTCTTTTACTTCCTTCTGGAAGTAGTGGACTTCCAATGGTTGCAAATGATGGTAGAGCAGGCTCTTCCTTGGAAGGCCGTCGTGCACCCTGCAAAAGAAAATCTATTGAAGGAAATGTTGCACAGTCCTCTTTAAGCGGAAGCAGTAGCTACTCTCAGCATATTGAAAGGGGTGCTGAGCCCCCTCTTCCAGCATTACCTGGTCGCTACAACACAGGAAGTAGGTTAAGCATACCTGCTCCACTAGCACGAATGAATCAAGCCCCTATACGAGGTACAGGTGAATTGATGTCCAATAGCTTCTCTGAGTCGATTGTTGCGGAAAACTCAGACAGTTCTCAACGAAACTATCGTATACGGATTAGTTCATCAAATGCACAAGATTCTTTTGCATCGGTAGGAACCGTTGTTAGGCATCCTGGGGGCCCATCTTCCCAGCCGCCAGCAAGACTTCTACCTGCTGAGCATGTATTAGACTTGAGGCCAGCAGCAGCTGTAGACAATTTATCTGCTCAAGGTCAGCCTATTATGATACACGTGCCTGCTTTATCTCGAAGTCTGCAGCCATATAGGTGGATTAGAAGTTCTACTTCAAGAAGTGGTAATTCATCAAGCTCTGCAGGAGAGAGGCAGGTGGTGCAACGTGAGGAGGTGAGACCAAACAACATGGGAAGAAACGTATCTGAACATCCTATGTTTGTCCCAGCAAATGAGCTGAGGAATATAGTTCAAAATCAATCAAGTAGAGGTTTAACATCTTCGAGCTTGCCCATTCCTGGAAATGTTGCTTCTACATCCAGGGTTGGTTCAAGTTCAGGGGTCCATCCCTCTTCTGGACCTTGGATTCCTCCTGAAAGTTCTCCGACACAGTTTCCCAGGAGGTTAACAGAATATGTTCGTAGGCAGCTATTTTCTTCTGCAACAAATGAGTCTGGGGGAAGGATAAATAATTATTCACAGCGATCAGGTTCTACTTTGGCTCAGGATATGGTTCTTTCATCTGGATCTGACCGCCGGGGGCACCATTTATTGCAACCGAGGTCAGCATTATGGATGGAAAGAAGAGGTGATGGTGGACTTGGTCTTCCCTATTCATTACGAACCTTGGCTACTTCTAATGAAGGAAGTGACAACAATAGACTTGTATCTGAGGTATGTTAATATTTTTCATGGAGTAATTGAAGGACGGCCCTATATGCATATATGTGCTCTGTCTGGATGCCTGTCTGCATCTTCAACGCAAAGCTGTTCATTTTGATTGATGAATAATTTGAACATCATTGGTCCGTGAATTCTTTTTATTGCAAATCCCTTTATTCAACCAATTAAAAATAATTTCTACTATTGTGAGTATCTATGAAGGTTCTCTTTTAATGCAAGCCTTTATAAAATGAAAGTTGCCATTTCTAAGTTGCTGGATTGAATTTTTCTGGGGGCAGGTGTGCGTTTCAGTAGGTAAGTGCCCTGGTGGGGGTTGTGCCTGCTAGGATGGTGTTTGCTGGACTATAAAATGCAGTTAAATTCTTTGAGAAATCCTATTTGGCATGAGCTATATTCTATTACAAGCAACCTTCTTTTTATCAATCTTGCTCTAGTTGTTTTCAGTGAAAAAAATCTTGCTCCAATTCTAAATAAACTTACATCACAAGTATGGATTGTGCTATAAAAAAGAGTTGATGGATTGTAATGCTGTCTTTGACTGTTTAGTTTACCGTTGGTAGTCTATCATCCATTGATTCTCAGCGATCTTACCTTTTGAAATGAATATGAAGCTCACATGTTATTAATTTTGTCTGCAGCAGATTCGCAATGTCTTGGGCCTTGTTCGCAGGGGTGAAAGCTTACGAGTTGAGGTAGGATCCTTATTGGTTATGTATCATGGTCAAGTTGTGCATTGGCTAATTTACATGGTTGCAATGAGATACAAGCAGCCTCTATGTTGCATGTTTTGTTTTTGTTTAGATTAGTTACAATGTGAAAATGGTGGTGAACAGCAACTTGTCTTGTTCATACTCCAGTATTCTTTTTCTAGTCCACAGATAGAAATAAAGCTTTCAGGTGCGCTTTTACTTGGGTAATATCAAAATGGTTCACTTTAGTTGATATGAATAGCCTTTATGATAGTTTAGGACAGGGCCTTGTGTTTATGTAATTATGCCTCCTTGGCTTGAAACTTCATTTGGGGCTCTGAGATATTTGTACATGGTCTAATGTTGCTTTATAATTTGTTTTATTAGGATGTGATGATCCTGGACCAGTCACTATTTTTTGGGATGGCTGATATGTATGATAGGCACAGGGATATGCGTCTGGACGTTGATAACATGTCATATGAGGTGGGCCTGATGCAAACTAACCAACTGAAGCCATTATTTCGCACCTTTTTCTTGTTGGTTTGTTCATGTTCAAAGTATGTTAAGTGCAGGAATTGTTGGCATTGGAAGAGCGTATCGGAAATGTCAACACAGGACTAAGTGAAGAGACGATAGTTGGTCGACTGAAACAAAAGAAGCATGTGAATGCTGTTGAATCACAGGTGGAGGAAGAACCATGCTGTGTTTGTCAGGTTGTAACAGATTCATTCATACTCATACGTGTTCTTTGAGCTTTGTTATCACTCTTGAACCCGCCTGGTTTGATTGGTGTCGACTAACAACCCACCTGATTTCCAGGAGGAATACGTCGAAGGAGATGACATGGGAACACTGGAATGCGGGCACGATTTCCACACGGATTGCATTAAGCAGTGGTTGATGCAGAAGAATCTGTGTCCCATCTGCAAAACAACTGGCATAGCAAGCCGAGAATGATGCTTGCTGATGCTGGAAAGGAGTGGAAAGAAGCAAGCAAGTGCAAAACGAGGGGGGACGAAATGCCATGGTGTTGCTTGCCAAATCTCTACCTTTACCTCTGGTCGCAAAAATGGAGGAGCCAGCCACATTCAAGTCGCTCTATATACATTTCTACAAGTAGTAAAATTAAGCAATCATTTTTTTCTTTTTTCTTTTTTTTTTTTTCTTTGGTTTTATTCTGGAAGAACAAAAATTCAAGGGTTGTAATTGGCAAGGAATTGTTTGTTAGCTTCTCAAGGTGTTATGGATGGTGGTTGGTCAAACTAATTAATGTTTACTCCCATTGACATTAGAGATTATTGGTCCTTAAAGTATCTCATCATCGGATTTTCCCATATTTTTAG

mRNA sequence

ATGCAAGGGCAAAGGAGTAGTGTCGGTTCATTGTCAGAAACAATTAACTTTGAACATGGATCTTCATCAAATAATCCTGGTAACCAACCAGTTTACTGGAATAACATGTGGAATCCAGCAGAAAACCGAATACCAGATTATTTATTGCCTACTTCTGATGTAAATTCTGGATATGTAAGCTCTGTAAGCCATGAACAACGAAGTTTGAGCAGATGGAGCTTGGGTGAACCTAGCTCTTGTGATATGCAAACTGATGTGCTGCCTGATGAGCAGAAAGCTGAGCTTGGATGGTCTTCAATGACTCGTGATGCAGATGGTCCCTTCACCGAAAACCAACTCTGTGAGCCATCAAATAATCCTTCACTGGGTCATGTGAATTTGAGCCCACTGATTATACAAAATTCTAATTCTAATAGTATTCCTCATACTCTGAATTTAAATGCAAGTTTTGCTAGTCATGGAGGTGACAGTAGCAGAGTTAACGAAGGTACTAACGTGTATAAATCGAGTGGATCAGAAGAAGGGGGGATTTTATGTTCTAGTGCATCTGATCCTCTTTTACTTCCTTCTGGAAGTAGTGGACTTCCAATGGTTGCAAATGATGGTAGAGCAGGCTCTTCCTTGGAAGGCCGTCGTGCACCCTGCAAAAGAAAATCTATTGAAGGAAATGTTGCACAGTCCTCTTTAAGCGGAAGCAGTAGCTACTCTCAGCATATTGAAAGGGGTGCTGAGCCCCCTCTTCCAGCATTACCTGGTCGCTACAACACAGGAAGTAGGTTAAGCATACCTGCTCCACTAGCACGAATGAATCAAGCCCCTATACGAGGTACAGGTGAATTGATGTCCAATAGCTTCTCTGAGTCGATTGTTGCGGAAAACTCAGACAGTTCTCAACGAAACTATCGTATACGGATTAGTTCATCAAATGCACAAGATTCTTTTGCATCGGTAGGAACCGTTGTTAGGCATCCTGGGGGCCCATCTTCCCAGCCGCCAGCAAGACTTCTACCTGCTGAGCATGTATTAGACTTGAGGCCAGCAGCAGCTGTAGACAATTTATCTGCTCAAGGTCAGCCTATTATGATACACGTGCCTGCTTTATCTCGAAGTCTGCAGCCATATAGGTGGATTAGAAGTTCTACTTCAAGAAGTGGTAATTCATCAAGCTCTGCAGGAGAGAGGCAGGTGGTGCAACGTGAGGAGGTGAGACCAAACAACATGGGAAGAAACGTATCTGAACATCCTATGTTTGTCCCAGCAAATGAGCTGAGGAATATAGTTCAAAATCAATCAAGTAGAGGTTTAACATCTTCGAGCTTGCCCATTCCTGGAAATGTTGCTTCTACATCCAGGGTTGGTTCAAGTTCAGGGGTCCATCCCTCTTCTGGACCTTGGATTCCTCCTGAAAGTTCTCCGACACAGTTTCCCAGGAGGTTAACAGAATATGTTCGTAGGCAGCTATTTTCTTCTGCAACAAATGAGTCTGGGGGAAGGATAAATAATTATTCACAGCGATCAGGTTCTACTTTGGCTCAGGATATGGTTCTTTCATCTGGATCTGACCGCCGGGGGCACCATTTATTGCAACCGAGGTCAGCATTATGGATGGAAAGAAGAGGTGATGGTGGACTTGGTCTTCCCTATTCATTACGAACCTTGGCTACTTCTAATGAAGGAAGTGACAACAATAGACTTGTATCTGAGATTCGCAATGTCTTGGGCCTTGTTCGCAGGGGTGAAAGCTTACGAGTTGAGGTAGGATCCTTATTGGTTATGTATCATGGTCAAGTTGTGCATTGGCTAATTTACATGTTACAATGTGAAAATGGTGGTGAACAGCAACTTGTCTTGTTCATACTCCAGTATTCTTTTTCTAGTCCACAGATAGAAATAAAGCTTTCAGGTGCGCTTTTACTTGGGACAGGGCCTTGTGTTTATGTAATTATGCCTCCTTGGCTTGAAACTTCATTTGGGGCTCTGAGATATTTGTACATGGATGTGATGATCCTGGACCAGTCACTATTTTTTGGGATGGCTGATATGTATGATAGGCACAGGGATATGCGTCTGGACGTTGATAACATGTCATATGAGGTGGGCCTGATGCAAACTAACCAACTGAAGCCATTATTTCGCACCTTTTTCTTGTTGGAATTGTTGGCATTGGAAGAGCGTATCGGAAATGTCAACACAGGACTAAGTGAAGAGACGATAGTTGGTCGACTGAAACAAAAGAAGCATGTGAATGCTGTTGAATCACAGGTGGAGGAAGAACCATGCTGTGTTTGTCAGGAGGAATACGTCGAAGGAGATGACATGGGAACACTGGAATGCGGGCACGATTTCCACACGGATTGCATTAAGCAGTGGTTGATGCAGAAGAATCTGTGTCCCATCTGCAAAACAACTGGCATAGCAAGCCGAGAATGATGCTTGCTGATGCTGGAAAGGAGTGGAAAGAAGCAAGCAAGTGCAAAACGAGGGGGGACGAAATGCCATGGTGTTGCTTGCCAAATCTCTACCTTTACCTCTGGTCGCAAAAATGGAGGAGCCAGCCACATTCAAGTCGCTCTATATACATTTCTACAAGTAGTAAAATTAAGCAATCATTTTTTTCTTTTTTCTTTTTTTTTTTTTCTTTGGTTTTATTCTGGAAGAACAAAAATTCAAGGGTTGTAATTGGCAAGGAATTGTTTGTTAGCTTCTCAAGGTGTTATGGATGGTGGTTGGTCAAACTAATTAATGTTTACTCCCATTGACATTAGAGATTATTGGTCCTTAAAGTATCTCATCATCGGATTTTCCCATATTTTTAG

Coding sequence (CDS)

ATGCAAGGGCAAAGGAGTAGTGTCGGTTCATTGTCAGAAACAATTAACTTTGAACATGGATCTTCATCAAATAATCCTGGTAACCAACCAGTTTACTGGAATAACATGTGGAATCCAGCAGAAAACCGAATACCAGATTATTTATTGCCTACTTCTGATGTAAATTCTGGATATGTAAGCTCTGTAAGCCATGAACAACGAAGTTTGAGCAGATGGAGCTTGGGTGAACCTAGCTCTTGTGATATGCAAACTGATGTGCTGCCTGATGAGCAGAAAGCTGAGCTTGGATGGTCTTCAATGACTCGTGATGCAGATGGTCCCTTCACCGAAAACCAACTCTGTGAGCCATCAAATAATCCTTCACTGGGTCATGTGAATTTGAGCCCACTGATTATACAAAATTCTAATTCTAATAGTATTCCTCATACTCTGAATTTAAATGCAAGTTTTGCTAGTCATGGAGGTGACAGTAGCAGAGTTAACGAAGGTACTAACGTGTATAAATCGAGTGGATCAGAAGAAGGGGGGATTTTATGTTCTAGTGCATCTGATCCTCTTTTACTTCCTTCTGGAAGTAGTGGACTTCCAATGGTTGCAAATGATGGTAGAGCAGGCTCTTCCTTGGAAGGCCGTCGTGCACCCTGCAAAAGAAAATCTATTGAAGGAAATGTTGCACAGTCCTCTTTAAGCGGAAGCAGTAGCTACTCTCAGCATATTGAAAGGGGTGCTGAGCCCCCTCTTCCAGCATTACCTGGTCGCTACAACACAGGAAGTAGGTTAAGCATACCTGCTCCACTAGCACGAATGAATCAAGCCCCTATACGAGGTACAGGTGAATTGATGTCCAATAGCTTCTCTGAGTCGATTGTTGCGGAAAACTCAGACAGTTCTCAACGAAACTATCGTATACGGATTAGTTCATCAAATGCACAAGATTCTTTTGCATCGGTAGGAACCGTTGTTAGGCATCCTGGGGGCCCATCTTCCCAGCCGCCAGCAAGACTTCTACCTGCTGAGCATGTATTAGACTTGAGGCCAGCAGCAGCTGTAGACAATTTATCTGCTCAAGGTCAGCCTATTATGATACACGTGCCTGCTTTATCTCGAAGTCTGCAGCCATATAGGTGGATTAGAAGTTCTACTTCAAGAAGTGGTAATTCATCAAGCTCTGCAGGAGAGAGGCAGGTGGTGCAACGTGAGGAGGTGAGACCAAACAACATGGGAAGAAACGTATCTGAACATCCTATGTTTGTCCCAGCAAATGAGCTGAGGAATATAGTTCAAAATCAATCAAGTAGAGGTTTAACATCTTCGAGCTTGCCCATTCCTGGAAATGTTGCTTCTACATCCAGGGTTGGTTCAAGTTCAGGGGTCCATCCCTCTTCTGGACCTTGGATTCCTCCTGAAAGTTCTCCGACACAGTTTCCCAGGAGGTTAACAGAATATGTTCGTAGGCAGCTATTTTCTTCTGCAACAAATGAGTCTGGGGGAAGGATAAATAATTATTCACAGCGATCAGGTTCTACTTTGGCTCAGGATATGGTTCTTTCATCTGGATCTGACCGCCGGGGGCACCATTTATTGCAACCGAGGTCAGCATTATGGATGGAAAGAAGAGGTGATGGTGGACTTGGTCTTCCCTATTCATTACGAACCTTGGCTACTTCTAATGAAGGAAGTGACAACAATAGACTTGTATCTGAGATTCGCAATGTCTTGGGCCTTGTTCGCAGGGGTGAAAGCTTACGAGTTGAGGTAGGATCCTTATTGGTTATGTATCATGGTCAAGTTGTGCATTGGCTAATTTACATGTTACAATGTGAAAATGGTGGTGAACAGCAACTTGTCTTGTTCATACTCCAGTATTCTTTTTCTAGTCCACAGATAGAAATAAAGCTTTCAGGTGCGCTTTTACTTGGGACAGGGCCTTGTGTTTATGTAATTATGCCTCCTTGGCTTGAAACTTCATTTGGGGCTCTGAGATATTTGTACATGGATGTGATGATCCTGGACCAGTCACTATTTTTTGGGATGGCTGATATGTATGATAGGCACAGGGATATGCGTCTGGACGTTGATAACATGTCATATGAGGTGGGCCTGATGCAAACTAACCAACTGAAGCCATTATTTCGCACCTTTTTCTTGTTGGAATTGTTGGCATTGGAAGAGCGTATCGGAAATGTCAACACAGGACTAAGTGAAGAGACGATAGTTGGTCGACTGAAACAAAAGAAGCATGTGAATGCTGTTGAATCACAGGTGGAGGAAGAACCATGCTGTGTTTGTCAGGAGGAATACGTCGAAGGAGATGACATGGGAACACTGGAATGCGGGCACGATTTCCACACGGATTGCATTAAGCAGTGGTTGATGCAGAAGAATCTGTGTCCCATCTGCAAAACAACTGGCATAGCAAGCCGAGAATGA

Protein sequence

MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVSSVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNPSLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIERGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRNYRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPANELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLTEYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRGDGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGALRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELLALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGHDFHTDCIKQWLMQKNLCPICKTTGIASRE
Homology
BLAST of CmoCh05G001710 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.7e-90
Identity = 283/828 (34.18%), Postives = 403/828 (48.67%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQG+R+S+G LSE +NFEHGSSS+N      +W N+ +  +N + DY++  S+ N+   +
Sbjct: 1   MQGERASLGYLSEALNFEHGSSSSN--GVIDHWENIHSLGDNDLQDYMIANSESNTSLAN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLP-DEQKAELGWSSMTRDADGPFTENQLCEPSNN 120
           SV HEQ+ L R+SLGE SS   + +    +EQ+ E      TR  DG   E         
Sbjct: 61  SVYHEQQGLRRFSLGEASSSGTKDEASSHNEQRME------TRCFDGRGNE--------- 120

Query: 121 PSLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKS-SGSEEGGIL 180
                ++L P+  Q S +N     +NLNA +         ++E  N Y+  SG  E    
Sbjct: 121 ----IIDLDPVFAQPSGTNQPVQNVNLNAEYI-------EIHEDINPYRGRSGFIEANGP 180

Query: 181 CSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQH 240
            +  S P        G     N    GSS+EGRRA CKRK++EG+++QSS  G   +   
Sbjct: 181 GTRVSQP--------GRSFEENGVGTGSSVEGRRASCKRKALEGSISQSSSGGYHDF--- 240

Query: 241 IERGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQ 300
            +RG           +  G+ L+I   L    +  + GT      S     V+  ++SS 
Sbjct: 241 -QRGESSSWTPGSTVFRPGNGLNISGSLDNGPRGMVSGTVPNFPVSAPNFPVSAIAESSS 300

Query: 301 RNYRIRISSSNAQD----SFASVGTVVRHPGGPSSQPPARLLPA-EHVLDLRPAAA-VDN 360
           RN  +R + S+ Q+    S  + GTVVR P  PS    +R LPA +H LDLRP  + V +
Sbjct: 301 RNICVRSNPSDHQETVNPSTFAAGTVVRRPVPPSQLNLSRHLPADQHSLDLRPGQSFVVS 360

Query: 361 LSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVS 420
            +    P+ I  P     L P+RW  SS    G S+S+A   + +  +E R  ++  N  
Sbjct: 361 RNPNSTPVSI-PPGSRTMLPPFRWTGSSLV-GGTSNSTAPVERNLHLDETRSRSIPGNTL 420

Query: 421 EHPMFVPANELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGP-----WIP 480
           E PMF  A E+ N  ++QSSR +T+ +L    + +S SR GS++ V P   P     W  
Sbjct: 421 EIPMFA-APEVGNFARSQSSRNVTNGNL---NSASSVSRTGSTTSVPPPPPPSSNLAWTS 480

Query: 481 PESSPTQFPRRL--TEYVRRQLFSS----ATNESGGRINNYSQRSGSTLAQDMVLSSGSD 540
            ++SP    RR   +E  RR L SS    ATN+  G        +    +  +VL  G D
Sbjct: 481 YQNSPHYQRRRTERSELARRSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGD 540

Query: 541 R-RGHHLLQPRSALWMERRGDGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRR-- 600
             + H+    R+    +R+GD  +G+P+ LR LA ++ G  +  +VS+++NVL ++RR  
Sbjct: 541 NSQMHNRAYSRAGPLFDRQGDSVVGIPHPLRALAAASRGR-SRLMVSQMQNVLDVMRRDA 600

Query: 601 -GESLRVEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGAL 660
              +LR+E                                                    
Sbjct: 601 NNNNLRLE---------------------------------------------------- 660

Query: 661 LLGTGPCVYVIMPPWLETSFGALRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSY 720
                                       DVM+L+ S+ F  A  +DR+RDMRLDVDNMSY
Sbjct: 661 ----------------------------DVMLLNHSVLFDGATGHDRYRDMRLDVDNMSY 682

Query: 721 EVGLMQTNQLKPLFRTFFLLELLALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEE 780
           E                   ELLALEERIG+V TG++EETI  RLKQ+K+ +  +S  + 
Sbjct: 721 E-------------------ELLALEERIGDVCTGVNEETISNRLKQRKYKSNTKSPQDA 682

Query: 781 EPCCVCQEEYVEGDDMGTLECGHDFHTDCIKQWLMQKNLCPICKTTGI 806
           EPCCVCQEEY EG+DMGTLECGH+FH+ CIK+WL QKNLCPICKTTG+
Sbjct: 781 EPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNLCPICKTTGL 682

BLAST of CmoCh05G001710 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 9.9e-67
Identity = 264/825 (32.00%), Postives = 353/825 (42.79%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQG RS+ G  S  IN+  G    +  N     NN+ NP + + P+    T      Y S
Sbjct: 1   MQGPRST-GDSSTGINYADGEPICST-NSETTSNNILNPVDVQFPNN--TTGSGRPTYAS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           S SH  ++ + WS GE SS    +D L               +++G  T+ QL       
Sbjct: 61  SSSHVVQNHNWWSFGESSSRLGPSDHL---------------NSNGSKTDRQLLSDGYGF 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
             G   +            +P    L  S +SH    S VN G ++   SG +  G++  
Sbjct: 121 EEGQSGM-----------LLPGESFLRGSSSSH--MLSHVNLGKDMDIGSGLQTSGVVIR 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
             +    L  GSS           GSSL G  + CKRK++EG  + S    S       E
Sbjct: 181 HNNCETSL--GSSSQTAEERSSGPGSSLGGLGSSCKRKALEGAPSHSFPGESHGCFFQTE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARM----NQA----PIRGTG---ELMSNSFSESI 300
            GA     A   +Y+  S LS+  P        NQ+    P  G G    + +++F  + 
Sbjct: 241 NGAWNEGLA---QYDASSSLSLSMPSQNSPNVNNQSGLPEPRFGLGGGRAVTASAFPSTR 300

Query: 301 VAENSDSSQRNYRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAA 360
             E      R         +   SF   G+ VR       Q PA   P    LD R    
Sbjct: 301 STETISRPGRRLNPGQPPESVAFSFTQSGSSVRQ----QQQLPA-TSPFVDPLDARAIPV 360

Query: 361 V-DNLSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMG 420
              + S  GQP MIH+PAL+R++  + W  SS+SR+ +          +  E + P +  
Sbjct: 361 TGSSSSGDGQPSMIHLPALTRNIHQFAWSASSSSRANS----------MPEEGLSPWDAP 420

Query: 421 RNVSEHPMF-VPANELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVH--PSSGPW 480
           R  SE P+F  PANE RN VQ+Q     T  +    G+     R GSSSG+H    +  W
Sbjct: 421 RINSEQPVFTTPANETRNPVQDQFCWSFTRGNPSTSGDSPFVPRAGSSSGIHGLQPNPTW 480

Query: 481 IPPESSPTQFPRRLTEYVRRQLFSSATNESG--GRINNYSQRSGSTLAQDMVLSSGSDRR 540
           + P +       R++E     LF S  +ES   G          S  + +    SGS  R
Sbjct: 481 VTPHNQ-----SRISEVAPWSLFPSIESESATHGASLPLLPTGPSVSSNEAAAPSGSSSR 540

Query: 541 GHHLLQPRSALWMERRGDGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLR 600
            H   Q RS L +ER+ D  L L +  R+LA  N+G   NRL+SEIR VL  +RRGE+LR
Sbjct: 541 SHRSRQRRSGLLLERQND-HLHLRHLGRSLAADNDG--RNRLISEIRQVLSAMRRGENLR 600

Query: 601 VEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGP 660
            E                                                          
Sbjct: 601 FE---------------------------------------------------------- 660

Query: 661 CVYVIMPPWLETSFGALRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQ 720
                                 D M+ D  ++ GMA+M+DRHRDMRLDVDNMSYE     
Sbjct: 661 ----------------------DYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYE----- 666

Query: 721 TNQLKPLFRTFFLLELLALEERIGNVNTGLSEETIVGRLKQKKHV-NAVESQVEEEPCCV 780
                         ELLAL ERIG+V+TGLSEE I+  +KQ KH  +A  S  + EPCCV
Sbjct: 721 --------------ELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCV 666

Query: 781 CQEEYVEGDDMGTLECGHDFHTDCIKQWLMQKNLCPICKTTGIAS 808
           CQEEY EGDD+GTL CGH+FHT C+KQWLM KNLCPICKT  +++
Sbjct: 781 CQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVALST 666

BLAST of CmoCh05G001710 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 2.9e-66
Identity = 262/840 (31.19%), Postives = 368/840 (43.81%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNP-GNQPVYWNNMWNPAE-NRIPDYLLPTSDVNSGY 60
           MQGQ++SV  L++   F+H SSS NP  +Q  YWNN+    E + +  Y +  SD    Y
Sbjct: 1   MQGQKNSVEQLADVFGFDHASSSGNPVMDQQGYWNNILGSVESHNLQGYQVNRSDGTIPY 60

Query: 61  VSSVSHEQRSLSRWSLGEPSSCDMQTDV-LPDEQKAELGWSSMTRDADGPFT--ENQLCE 120
            + V      L  W  GE S+          +E KAE       R+  G     E +L  
Sbjct: 61  GNGVHQNGTFLGFWESGEASASGSSLHFGGSNEIKAE------QRNIGGGLRIGERRLVA 120

Query: 121 PSN----NPSLG-HVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNE------GT 180
             N    N  +G ++N + L  +NSN N       L+    SH G + + +E       T
Sbjct: 121 ERNLSLDNVDIGLNINGNDLSGENSNVNGASQGSELHGG-CSHTGSNGQASELRLHPYRT 180

Query: 181 NVYKSSGSEEGGILCSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNV 240
            +  +   E    L  S +     P G   L     D R GSSL+GRR  CKRK+IEG  
Sbjct: 181 FILGADQPEPFNSLNGSEN-----PLGDFSLMPEGIDQRPGSSLDGRRLACKRKNIEGVN 240

Query: 241 AQSSLSGSSSYS-------QHIERGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRG- 300
            Q S   S+S+S        +I   +  P P+     N  S   +  P     Q P  G 
Sbjct: 241 GQCSAGASTSFSHRNDSIFHNIASSSHNPSPST----NLPSPNCLLVPSTLDEQLPRYGA 300

Query: 301 -TGELMSNSFSESIVAENSDSSQRNYRIRISSSNAQDSFA--SVGTVVRHPGGPSSQPPA 360
            T  L S+S+  S    NS  SQR++R R S +     +      + +RH    + QPP 
Sbjct: 301 TTAGLSSSSYDPSGGNNNSGGSQRSFRPRTSLAQHIGPYGVWPSSSTIRHSNSWNHQPP- 360

Query: 361 RLLPAEHVLDLRPAA--AVDNLSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSA 420
              P +   D  P     V +L+ Q Q  M  VP + +    +    +S+SR+GN     
Sbjct: 361 ---PFQSSFDEPPEVIPVVSSLNFQYQHPMNVVPGIPQMSHRFTGPGASSSRTGNL---- 420

Query: 421 GERQVVQREEVRPNNMGRNVSEHPMFVP--ANELRNIVQNQSSRGLTSSSLPIPGNVAST 480
            E +++  EE    N+    +  P  VP  A ++R+++   SS  +   +  IPGNV S+
Sbjct: 421 -ENRIIGSEEFSARNV--VATSFPDAVPPAALDMRHLIPEPSSWNVDGRATTIPGNVPSS 480

Query: 481 SRVGSSSGVHPSSG-PWIPPESSPTQFPRRLTEYVRRQLFSSATNESGGRINNYSQRSGS 540
           SR  ++S V+P +G P+I  ++   + PR L+E +                   S+ SG+
Sbjct: 481 SRANTNSMVNPPAGSPFIAHQNLHRRNPRNLSEEI-------------------SRLSGA 540

Query: 541 TLAQDMVLSSGSDRRGHHLLQPRSALWMERRGDGGLGLPYSLRTLATSNEGSDNNRLVSE 600
                         RGH   + RS   +ER+GDG  G+P S R    S EG    R + E
Sbjct: 541 L-------------RGHQHPRLRSGFLLERQGDGVWGVPLSTR----SREG----RRLIE 600

Query: 601 IRNVLGLVRRGESLRVEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLVLFILQYSFSSPQ 660
           IRN L ++ RGE++R E                                           
Sbjct: 601 IRNALEMIHRGENVRFE------------------------------------------- 660

Query: 661 IEIKLSGALLLGTGPCVYVIMPPWLETSFGALRYLYMDVMILDQSLFFGMADMYDRHRDM 720
                                                       S+F+G  D++DRHRDM
Sbjct: 661 --------------------------------------------SIFYGGVDIHDRHRDM 667

Query: 721 RLDVDNMSYEVGLMQTNQLKPLFRTFFLLELLALEERIGNVNTGLSEETIVGRLKQKKHV 780
           RLD+DNMSYE                   ELLALEERIGNV+TGLSEE +   LKQ+K  
Sbjct: 721 RLDIDNMSYE-------------------ELLALEERIGNVSTGLSEEEVTKLLKQRKFS 667

Query: 781 N-AVESQVEEEPCCVCQEEYVEGDDMGTLECGHDFHTDCIKQWLMQKNLCPICKTTGIAS 808
           +  +E+ VEEEPCC+CQEEYV+GDD+GTL+CGHDFH  C++QWL+ KN CPICK T + S
Sbjct: 781 SWRLEASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTALKS 667

BLAST of CmoCh05G001710 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 6.2e-61
Identity = 258/853 (30.25%), Postives = 363/853 (42.56%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVY-----WNNMWNPAENRIPDYLLPTSDVN 60
           MQG RS  GS +E          N    + +Y      N M NPA+   P+   P+    
Sbjct: 4   MQGPRSIGGSSTEV---------NQVDGESIYCTETSLNTMLNPADTGFPNNSTPSG--R 63

Query: 61  SGYVSSVSHEQRSLSRWSLGEPSSCDMQTDVL------------PDEQKAELGWSSMTRD 120
             Y SS SH  +  + W  GE SS    +D +             D     +G+ S  R 
Sbjct: 64  PTYASSSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQ 123

Query: 121 ADGPFTENQLCEPSNNPSLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEG 180
                  N +       +  H+   P  ++ S+SN +P  ++++        DS   N  
Sbjct: 124 TG----LNGMMVDGGVHAGSHIRNVPSFLRGSSSNPMPQHVDMSMDM-----DSDNCNAQ 183

Query: 181 TNVYKSSGSEEGGILCSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGN 240
           T+      +  G  L SS        +G S     +  G  GSS       CKRK++EG+
Sbjct: 184 TSGVVIRHNSYGSSLGSSVQ-----AAGESSSGPASPFGGWGSS-------CKRKALEGS 243

Query: 241 VAQSSLSGSSSYSQHIERGAEPPLPALPGRYNTGSRLSIPAP----------LARMNQAP 300
            +      + +     E  A     A   +Y   S LS+  P            R  Q  
Sbjct: 244 PSHYFSGETPNRIVQTENSAS---HASLSQYGASSSLSLATPSQSSPNVTNHFGRTEQMF 303

Query: 301 IRGTGELMSNSFSESIVAENSDSSQRNYRIRISSSNAQDSFA-SV---GTVVRHPGGPSS 360
             G G  ++ S   S    N+D+  R  R R++    Q+S A SV   GT VR  G    
Sbjct: 304 GSGGGRAVAASAFHS--TRNTDTLSRAGR-RLNPRQPQESVAFSVSHGGTSVRPTGSLQQ 363

Query: 361 QPPARLLPAEHVLDLRPAAAVD-NLSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSS 420
             P    P     D+R ++    + + + Q  ++H+PAL+R++  Y W  S +SR+ N S
Sbjct: 364 NLPLN-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRASNPS 423

Query: 421 --SSAGERQVVQREEVRPNNMGRNVSEHPMFVPANELRNIVQN-----QSSRGLTSSSLP 480
                 ER   Q E  R N       E P+F PA ++R  V +     + S G +  SL 
Sbjct: 424 GIGMPAERLGPQWETPRSN------QEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLF 483

Query: 481 IPGNVASTSRVGSSSGVH-PSSGP-WIPPESSPTQFPRRLTEYVRRQLFSSATNES---G 540
           +P       R G SS +H P   P WIPP+++P   P R +E     LF S  + S   G
Sbjct: 484 VP-------RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHG 543

Query: 541 GRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRGDGGLGLPYSLRTLATS 600
           G +        S  + ++ + S S+ R H     RS L +ER+ +  L L +  R+LA  
Sbjct: 544 GPL-PLLPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNE-LLHLRHIGRSLAA- 603

Query: 601 NEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLV 660
            +G+  N+++SEIR VL  +RRGE+LRVE                               
Sbjct: 604 -DGNGRNQIISEIRQVLHAMRRGENLRVE------------------------------- 663

Query: 661 LFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGALRYLYMDVMILDQSLFF 720
                                                            D M+ D  ++ 
Sbjct: 664 -------------------------------------------------DYMVFDPLIYQ 701

Query: 721 GMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELLALEERIGNVNTGLSEE 780
           GM DM+DRHR+MRLDVDNMSYE                   ELLAL ERIG+V+TGLSEE
Sbjct: 724 GMTDMHDRHREMRLDVDNMSYE-------------------ELLALGERIGDVSTGLSEE 701

Query: 781 TIVGRLKQKKHVNAVESQVE----EEPCCVCQEEYVEGDDMGTLECGHDFHTDCIKQWLM 806
            I+  +KQ KH ++  S VE     EPCC+CQEEYVEGD++GTL+CGH+FH DCIKQW+M
Sbjct: 784 VILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVM 701

BLAST of CmoCh05G001710 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 2.8e-29
Identity = 68/147 (46.26%), Postives = 93/147 (63.27%), Query Frame = 0

Query: 669 ILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELLALEERIGN 728
           I+D S  + ++++ D HRDMRLD+D+M+YE                   ELLALEE+IG+
Sbjct: 391 IMDFSRLYEVSNVVDEHRDMRLDIDSMTYE-------------------ELLALEEQIGD 450

Query: 729 VNTGLSEETIVGRLKQKKHV--------NAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 788
           VNTGL++  IV +LK    V         + ES +E + C +CQEEY   + +GTL+CGH
Sbjct: 451 VNTGLAKSYIVEKLKTSLFVPGSSCMSNKSSESSMENDACIICQEEYQVKECIGTLDCGH 510

Query: 789 DFHTDCIKQWLMQKNLCPICKTTGIAS 808
            +H DCIKQWLM KNLCPICKTT +++
Sbjct: 511 RYHEDCIKQWLMVKNLCPICKTTALST 518

BLAST of CmoCh05G001710 vs. ExPASy TrEMBL
Match: A0A6J1HF13 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462926 PE=4 SV=1)

HSP 1 Score: 1343.2 bits (3475), Expect = 0.0e+00
Identity = 710/809 (87.76%), Postives = 710/809 (87.76%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHW 600
           DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE               
Sbjct: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE--------------- 600

Query: 601 LIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGAL 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 RYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELL 720
                DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   ELL
Sbjct: 661 -----DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------ELL 710

Query: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 780
           ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH
Sbjct: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 710

Query: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           DFHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 710

BLAST of CmoCh05G001710 vs. ExPASy TrEMBL
Match: A0A6J1HCX0 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462926 PE=4 SV=1)

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 710/810 (87.65%), Postives = 710/810 (87.65%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSE-IRNVLGLVRRGESLRVEVGSLLVMYHGQVVH 600
           DGGLGLPYSLRTLATSNEGSDNNRLVSE IRNVLGLVRRGESLRVE              
Sbjct: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEQIRNVLGLVRRGESLRVE-------------- 600

Query: 601 WLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGA 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 LRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLEL 720
                 DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   EL
Sbjct: 661 ------DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------EL 711

Query: 721 LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 780
           LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG
Sbjct: 721 LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 711

Query: 781 HDFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           HDFHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 HDFHTDCIKQWLMQKNLCPICKTTGIASRE 711

BLAST of CmoCh05G001710 vs. ExPASy TrEMBL
Match: A0A6J1KBL5 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491824 PE=4 SV=1)

HSP 1 Score: 1318.5 bits (3411), Expect = 0.0e+00
Identity = 697/809 (86.16%), Postives = 703/809 (86.90%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPD QKAELGWSS+TRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDGQKAELGWSSVTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGT+VYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTDVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPM+ANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMLANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIV ENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVVENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPG PSSQPPARLLPAEHVLDLRPAAAVDNLS QGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGAPSSQPPARLLPAEHVLDLRPAAAVDNLSTQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVP 
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPG 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRN+VQNQSSRGLTSSSLPIPGNVASTSRVGS SGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNMVQNQSSRGLTSSSLPIPGNVASTSRVGSISGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRG+HLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGNHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHW 600
           D GLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE               
Sbjct: 541 DSGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE--------------- 600

Query: 601 LIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGAL 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 RYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELL 720
                DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   ELL
Sbjct: 661 -----DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------ELL 710

Query: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 780
           ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH
Sbjct: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 710

Query: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           +FHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 NFHTDCIKQWLMQKNLCPICKTTGIASRE 710

BLAST of CmoCh05G001710 vs. ExPASy TrEMBL
Match: A0A6J1K2J8 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491824 PE=4 SV=1)

HSP 1 Score: 1313.9 bits (3399), Expect = 0.0e+00
Identity = 697/810 (86.05%), Postives = 703/810 (86.79%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPD QKAELGWSS+TRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDGQKAELGWSSVTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGT+VYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTDVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPM+ANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMLANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIV ENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVVENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPG PSSQPPARLLPAEHVLDLRPAAAVDNLS QGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGAPSSQPPARLLPAEHVLDLRPAAAVDNLSTQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVP 
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPG 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRN+VQNQSSRGLTSSSLPIPGNVASTSRVGS SGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNMVQNQSSRGLTSSSLPIPGNVASTSRVGSISGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRG+HLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGNHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSE-IRNVLGLVRRGESLRVEVGSLLVMYHGQVVH 600
           D GLGLPYSLRTLATSNEGSDNNRLVSE IRNVLGLVRRGESLRVE              
Sbjct: 541 DSGLGLPYSLRTLATSNEGSDNNRLVSEQIRNVLGLVRRGESLRVE-------------- 600

Query: 601 WLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGA 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 LRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLEL 720
                 DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   EL
Sbjct: 661 ------DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------EL 711

Query: 721 LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 780
           LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG
Sbjct: 721 LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 711

Query: 781 HDFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           H+FHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 HNFHTDCIKQWLMQKNLCPICKTTGIASRE 711

BLAST of CmoCh05G001710 vs. ExPASy TrEMBL
Match: A0A1S4DVI9 (probable E3 ubiquitin-protein ligase HIP1 OS=Cucumis melo OX=3656 GN=LOC103487873 PE=4 SV=1)

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 634/812 (78.08%), Postives = 672/812 (82.76%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNN+WNPAENRIPDYLLPTSDVNSGYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNVWNPAENRIPDYLLPTSDVNSGYVN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQT++LPDEQKAE+GWSSM  DADGP TEN+LCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTEMLPDEQKAEIGWSSMASDADGPVTENRLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNS--IPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGIL 180
           SLGHVNLSPLIIQNSNSNS  IPH LNLN+SF SHGGD+SRVNEGTNVYKSSG EEG IL
Sbjct: 121 SLGHVNLSPLIIQNSNSNSNAIPHNLNLNSSFVSHGGDNSRVNEGTNVYKSSGPEEGRIL 180

Query: 181 CSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQH 240
           CS+ASDPLLLPSG+SGLP+V NDGR GSSL+GRRAPCKRKSIEGNVAQSSLSGSS+YSQH
Sbjct: 181 CSNASDPLLLPSGNSGLPVVGNDGRPGSSLDGRRAPCKRKSIEGNVAQSSLSGSSNYSQH 240

Query: 241 IERGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQ 300
           IE G+E  LP  PGRYNTGSR+SIP P ARMNQAP+RG GEL SN+FSESIVAE+SDSSQ
Sbjct: 241 IESGSEAHLPVFPGRYNTGSRISIPPPPARMNQAPVRGAGELTSNNFSESIVAESSDSSQ 300

Query: 301 RNYRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQ 360
           RNYRIRISSSNAQDSFAS GT VRHPG PSSQ PARLLPAEH+LDLRPA AVDN+SAQGQ
Sbjct: 301 RNYRIRISSSNAQDSFASAGTAVRHPGPPSSQLPARLLPAEHILDLRPAPAVDNVSAQGQ 360

Query: 361 PIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFV 420
           PIMIHVPAL R+LQPYRW  SSTSRSG+SSSSAGERQVVQREEVRP+ MGRNVSEHPMF+
Sbjct: 361 PIMIHVPALPRNLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSIMGRNVSEHPMFL 420

Query: 421 PANELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR 480
           PANELR +V++ SSR L SS+L IPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR
Sbjct: 421 PANELRGLVRSPSSRALASSNLAIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR 480

Query: 481 LTEYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMER 540
           LTEYVRRQLF+SATNESGGR +NYSQRSGST AQ+MVLSSGS RRGHHLLQPRSALWMER
Sbjct: 481 LTEYVRRQLFASATNESGGRTSNYSQRSGSTSAQEMVLSSGSGRRGHHLLQPRSALWMER 540

Query: 541 RGDGGLGLPYSLRTLATSNEGSDNNRLVSE-IRNVLGLVRRGESLRVEVGSLLVMYHGQV 600
           RGDGGLGLPYSLRTLA+S EGSDNNRLVSE +RNVLGLVRRGESLRVE            
Sbjct: 541 RGDGGLGLPYSLRTLASSAEGSDNNRLVSEHLRNVLGLVRRGESLRVE------------ 600

Query: 601 VHWLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSF 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 GALRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLL 720
                   DVMILDQSLFFGMAD+YDRHRDMRLDVDNMSYE                   
Sbjct: 661 --------DVMILDQSLFFGMADIYDRHRDMRLDVDNMSYE------------------- 713

Query: 721 ELLALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLE 780
           ELLALEERIGNVNTGL+EETIV RLKQKKHVNAVESQVEEEPCCVCQEEYVEG+D+GTLE
Sbjct: 721 ELLALEERIGNVNTGLNEETIVARLKQKKHVNAVESQVEEEPCCVCQEEYVEGEDIGTLE 713

Query: 781 CGHDFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           CGHDFHT CIKQWLMQKNLCPICKTTG+ASRE
Sbjct: 781 CGHDFHTACIKQWLMQKNLCPICKTTGLASRE 713

BLAST of CmoCh05G001710 vs. NCBI nr
Match: XP_022962523.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 [Cucurbita moschata])

HSP 1 Score: 1343.2 bits (3475), Expect = 0.0e+00
Identity = 710/809 (87.76%), Postives = 710/809 (87.76%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHW 600
           DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE               
Sbjct: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE--------------- 600

Query: 601 LIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGAL 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 RYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELL 720
                DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   ELL
Sbjct: 661 -----DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------ELL 710

Query: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 780
           ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH
Sbjct: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 710

Query: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           DFHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 710

BLAST of CmoCh05G001710 vs. NCBI nr
Match: KAG6598360.1 (putative E3 ubiquitin-protein ligase RHG1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 708/809 (87.52%), Postives = 710/809 (87.76%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRN+SEHPMFVPA
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNISEHPMFVPA 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRN+VQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNMVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHW 600
           DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE               
Sbjct: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE--------------- 600

Query: 601 LIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGAL 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 RYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELL 720
                DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   ELL
Sbjct: 661 -----DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------ELL 710

Query: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 780
           ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH
Sbjct: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 710

Query: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           DFHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 710

BLAST of CmoCh05G001710 vs. NCBI nr
Match: XP_022962521.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_022962522.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 710/810 (87.65%), Postives = 710/810 (87.65%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSE-IRNVLGLVRRGESLRVEVGSLLVMYHGQVVH 600
           DGGLGLPYSLRTLATSNEGSDNNRLVSE IRNVLGLVRRGESLRVE              
Sbjct: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEQIRNVLGLVRRGESLRVE-------------- 600

Query: 601 WLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGA 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 LRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLEL 720
                 DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   EL
Sbjct: 661 ------DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------EL 711

Query: 721 LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 780
           LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG
Sbjct: 721 LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 711

Query: 781 HDFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           HDFHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 HDFHTDCIKQWLMQKNLCPICKTTGIASRE 711

BLAST of CmoCh05G001710 vs. NCBI nr
Match: KAG7029330.1 (putative E3 ubiquitin-protein ligase RHG1A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1335.5 bits (3455), Expect = 0.0e+00
Identity = 707/810 (87.28%), Postives = 710/810 (87.65%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRN+SEHPMFVPA
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNISEHPMFVPA 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRN+VQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNMVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSE-IRNVLGLVRRGESLRVEVGSLLVMYHGQVVH 600
           DGGLGLPYSLRTLATSNEGSDNNRLVSE IRNVLGLVRRGESLRVE              
Sbjct: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEQIRNVLGLVRRGESLRVE-------------- 600

Query: 601 WLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGA 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 LRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLEL 720
                 DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   EL
Sbjct: 661 ------DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------EL 711

Query: 721 LALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 780
           LALEERIGNVNTGLSEETIVGRLK+KKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG
Sbjct: 721 LALEERIGNVNTGLSEETIVGRLKRKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECG 711

Query: 781 HDFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           HDFHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 HDFHTDCIKQWLMQKNLCPICKTTGIASRE 711

BLAST of CmoCh05G001710 vs. NCBI nr
Match: XP_023545718.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1327.8 bits (3435), Expect = 0.0e+00
Identity = 703/809 (86.90%), Postives = 706/809 (87.27%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPT DVNSGYVS
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTPDVNSGYVS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           SVSHEQRSLSRWSLGEPSSCDMQTD+LPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTDLLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
           SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS
Sbjct: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
           SASDPLLLPSGSSGLPMVANDGRA SSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE
Sbjct: 181 SASDPLLLPSGSSGLPMVANDGRASSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300
           RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN
Sbjct: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQRN 300

Query: 301 YRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360
           YRIRISSSNAQDSFASVGTVVRHP GPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI
Sbjct: 301 YRIRISSSNAQDSFASVGTVVRHPRGPSSQPPARLLPAEHVLDLRPAAAVDNLSAQGQPI 360

Query: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420
           MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVSEHPMFVPA 420

Query: 421 NELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480
           NELRN+VQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT
Sbjct: 421 NELRNMVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSAL MERRG
Sbjct: 481 EYVRRQLFSSATNESGGRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALRMERRG 540

Query: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHW 600
           DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE               
Sbjct: 541 DGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVE--------------- 600

Query: 601 LIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGAL 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 660

Query: 661 RYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELL 720
                DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE                   ELL
Sbjct: 661 -----DVMILDQSLFFGMADMYDRHRDMRLDVDNMSYE-------------------ELL 710

Query: 721 ALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 780
           ALEERIGNVNTGLSEETI+GRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH
Sbjct: 721 ALEERIGNVNTGLSEETIIGRLKQKKHVNAVESQVEEEPCCVCQEEYVEGDDMGTLECGH 710

Query: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 810
           DFHTDCIKQWLMQKNLCPICKTTGIASRE
Sbjct: 781 DFHTDCIKQWLMQKNLCPICKTTGIASRE 710

BLAST of CmoCh05G001710 vs. TAIR 10
Match: AT5G42940.1 (RING/U-box superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 2.6e-91
Identity = 283/828 (34.18%), Postives = 403/828 (48.67%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQG+R+S+G LSE +NFEHGSSS+N      +W N+ +  +N + DY++  S+ N+   +
Sbjct: 1   MQGERASLGYLSEALNFEHGSSSSN--GVIDHWENIHSLGDNDLQDYMIANSESNTSLAN 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLP-DEQKAELGWSSMTRDADGPFTENQLCEPSNN 120
           SV HEQ+ L R+SLGE SS   + +    +EQ+ E      TR  DG   E         
Sbjct: 61  SVYHEQQGLRRFSLGEASSSGTKDEASSHNEQRME------TRCFDGRGNE--------- 120

Query: 121 PSLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKS-SGSEEGGIL 180
                ++L P+  Q S +N     +NLNA +         ++E  N Y+  SG  E    
Sbjct: 121 ----IIDLDPVFAQPSGTNQPVQNVNLNAEYI-------EIHEDINPYRGRSGFIEANGP 180

Query: 181 CSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQH 240
            +  S P        G     N    GSS+EGRRA CKRK++EG+++QSS  G   +   
Sbjct: 181 GTRVSQP--------GRSFEENGVGTGSSVEGRRASCKRKALEGSISQSSSGGYHDF--- 240

Query: 241 IERGAEPPLPALPGRYNTGSRLSIPAPLARMNQAPIRGTGELMSNSFSESIVAENSDSSQ 300
            +RG           +  G+ L+I   L    +  + GT      S     V+  ++SS 
Sbjct: 241 -QRGESSSWTPGSTVFRPGNGLNISGSLDNGPRGMVSGTVPNFPVSAPNFPVSAIAESSS 300

Query: 301 RNYRIRISSSNAQD----SFASVGTVVRHPGGPSSQPPARLLPA-EHVLDLRPAAA-VDN 360
           RN  +R + S+ Q+    S  + GTVVR P  PS    +R LPA +H LDLRP  + V +
Sbjct: 301 RNICVRSNPSDHQETVNPSTFAAGTVVRRPVPPSQLNLSRHLPADQHSLDLRPGQSFVVS 360

Query: 361 LSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMGRNVS 420
            +    P+ I  P     L P+RW  SS    G S+S+A   + +  +E R  ++  N  
Sbjct: 361 RNPNSTPVSI-PPGSRTMLPPFRWTGSSLV-GGTSNSTAPVERNLHLDETRSRSIPGNTL 420

Query: 421 EHPMFVPANELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPSSGP-----WIP 480
           E PMF  A E+ N  ++QSSR +T+ +L    + +S SR GS++ V P   P     W  
Sbjct: 421 EIPMFA-APEVGNFARSQSSRNVTNGNL---NSASSVSRTGSTTSVPPPPPPSSNLAWTS 480

Query: 481 PESSPTQFPRRL--TEYVRRQLFSS----ATNESGGRINNYSQRSGSTLAQDMVLSSGSD 540
            ++SP    RR   +E  RR L SS    ATN+  G        +    +  +VL  G D
Sbjct: 481 YQNSPHYQRRRTERSELARRSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGD 540

Query: 541 R-RGHHLLQPRSALWMERRGDGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRR-- 600
             + H+    R+    +R+GD  +G+P+ LR LA ++ G  +  +VS+++NVL ++RR  
Sbjct: 541 NSQMHNRAYSRAGPLFDRQGDSVVGIPHPLRALAAASRGR-SRLMVSQMQNVLDVMRRDA 600

Query: 601 -GESLRVEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGAL 660
              +LR+E                                                    
Sbjct: 601 NNNNLRLE---------------------------------------------------- 660

Query: 661 LLGTGPCVYVIMPPWLETSFGALRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSY 720
                                       DVM+L+ S+ F  A  +DR+RDMRLDVDNMSY
Sbjct: 661 ----------------------------DVMLLNHSVLFDGATGHDRYRDMRLDVDNMSY 682

Query: 721 EVGLMQTNQLKPLFRTFFLLELLALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEE 780
           E                   ELLALEERIG+V TG++EETI  RLKQ+K+ +  +S  + 
Sbjct: 721 E-------------------ELLALEERIGDVCTGVNEETISNRLKQRKYKSNTKSPQDA 682

Query: 781 EPCCVCQEEYVEGDDMGTLECGHDFHTDCIKQWLMQKNLCPICKTTGI 806
           EPCCVCQEEY EG+DMGTLECGH+FH+ CIK+WL QKNLCPICKTTG+
Sbjct: 781 EPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNLCPICKTTGL 682

BLAST of CmoCh05G001710 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-81
Identity = 263/819 (32.11%), Postives = 392/819 (47.86%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNP-GNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYV 60
           MQG+R+S+GSLS+ +NFE GS+S+N   +Q + W N+ N  +N + DY+   +D N  + 
Sbjct: 1   MQGERASLGSLSKALNFERGSTSSNAVVDQQIRWENLHNYGDNDLQDYMSSAADTNPTFA 60

Query: 61  SSVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNN 120
           +SV HE+R L R+++GE SS   + +     ++    W  + R            E   N
Sbjct: 61  NSVYHEKRGLQRFNIGEASSSGTKNEGASLTEQ----WKGIGR-----------FEEQRN 120

Query: 121 PSLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILC 180
             L    LSPL +Q SN + +   +NLNA +  H  D + V      ++ +G     +L 
Sbjct: 121 DKL---ELSPLFVQPSNGSRVVRDVNLNAEYNEHLEDMNPVTVHPGHFEVNGLRSEFLLD 180

Query: 181 SSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHI 240
           +S                     RAGSS++GRRA CKRK+++ +  QSS +G     +  
Sbjct: 181 NSV--------------------RAGSSVDGRRASCKRKALDASGGQSSSTGG---FREF 240

Query: 241 ERGAEPPLPALPGRYNTGSRLSIPAPLAR-MNQAPIRGTGELMSNSFSESIVAENSDSSQ 300
           +RG      + P  Y+       PA  A  +N +   G   L+S++         ++SS 
Sbjct: 241 QRGVSSSWISGPTYYS-------PAMTANDLNISLDHGRRGLVSSAVPNLSPPTITESSS 300

Query: 301 RNYRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEH-VLDLRPAAAVDNLSAQG 360
           RNY + ++ +  Q++ ++    +  PG         L+PA+H  + +R   A+ N ++Q 
Sbjct: 301 RNYPVWVNPTYQQETVSNFAPSLNSPG---------LIPADHQQISMRYGHALGNFASQN 360

Query: 361 -QPIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSAG-ERQVVQREEVRPNNMGRNVSEHP 420
                 H+P +SR+   ++W  S  +   +SSS+   +R V+ R   R  +   N  E P
Sbjct: 361 PNAPATHMPPVSRN--TFQWNTSPVAAVISSSSATPVDRNVIHRNATRQRS---NTLEIP 420

Query: 421 MFVPANELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVHPS-SGPWIPP-ESSPT 480
           +FVPA ELRN+     SR  + +      +VAS+S   S + V PS S P + P +++  
Sbjct: 421 LFVPAPELRNVAHGHISRNASGAR-----HVASSS---SRTSVQPSPSSPALTPYQNNSL 480

Query: 481 QFPRRLTEYVRRQLFSSATNESGGRINNYSQRSGSTLA-----QDMVLSSGSDRRG--HH 540
              RRL+E  RR L SS   +         QR+  +LA      + VL SG D     H+
Sbjct: 481 HNQRRLSENFRRSLLSSLVTQ---------QRAARSLAHPASPNEHVLQSGGDNTSQVHN 540

Query: 541 LLQPRSALWMERRGDGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLRVEV 600
               R+     R+G    G+ +SLR LA+++ G       SEIRN+L  +RR  +LR+E 
Sbjct: 541 RASSRAG---PRQGQDATGISHSLRGLASTSRGR-TRMGASEIRNILEHMRRAGNLRLE- 600

Query: 601 GSLLVMYHGQVVHWLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGPCVY 660
                                                                       
Sbjct: 601 ------------------------------------------------------------ 637

Query: 661 VIMPPWLETSFGALRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQTNQ 720
                              DVM+L+QS+  G AD++DR+RDMRLDVDNM+YE        
Sbjct: 661 -------------------DVMLLNQSIMLGAADIHDRYRDMRLDVDNMTYE-------- 637

Query: 721 LKPLFRTFFLLELLALEERIGNVNTGLSEETIVGRLKQKKHVNAVESQVEEEPCCVCQEE 780
                      ELL+LEERIG+V TGL+EETI  RLKQ+K+ ++  S  E EPCCVCQEE
Sbjct: 721 -----------ELLSLEERIGDVCTGLNEETISNRLKQQKYKSSTRSSQEVEPCCVCQEE 637

Query: 781 YVEGDDMGTLECGHDFHTDCIKQWLMQKNLCPICKTTGI 806
           Y E +++G LECGHDFH+ CIK+WL QKNLCPICKTTG+
Sbjct: 781 YKEEEEIGRLECGHDFHSQCIKEWLKQKNLCPICKTTGL 637

BLAST of CmoCh05G001710 vs. TAIR 10
Match: AT4G34040.1 (RING/U-box superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 7.0e-68
Identity = 264/825 (32.00%), Postives = 353/825 (42.79%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVYWNNMWNPAENRIPDYLLPTSDVNSGYVS 60
           MQG RS+ G  S  IN+  G    +  N     NN+ NP + + P+    T      Y S
Sbjct: 1   MQGPRST-GDSSTGINYADGEPICST-NSETTSNNILNPVDVQFPNN--TTGSGRPTYAS 60

Query: 61  SVSHEQRSLSRWSLGEPSSCDMQTDVLPDEQKAELGWSSMTRDADGPFTENQLCEPSNNP 120
           S SH  ++ + WS GE SS    +D L               +++G  T+ QL       
Sbjct: 61  SSSHVVQNHNWWSFGESSSRLGPSDHL---------------NSNGSKTDRQLLSDGYGF 120

Query: 121 SLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEGTNVYKSSGSEEGGILCS 180
             G   +            +P    L  S +SH    S VN G ++   SG +  G++  
Sbjct: 121 EEGQSGM-----------LLPGESFLRGSSSSH--MLSHVNLGKDMDIGSGLQTSGVVIR 180

Query: 181 SASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGNVAQSSLSGSSSYSQHIE 240
             +    L  GSS           GSSL G  + CKRK++EG  + S    S       E
Sbjct: 181 HNNCETSL--GSSSQTAEERSSGPGSSLGGLGSSCKRKALEGAPSHSFPGESHGCFFQTE 240

Query: 241 RGAEPPLPALPGRYNTGSRLSIPAPLARM----NQA----PIRGTG---ELMSNSFSESI 300
            GA     A   +Y+  S LS+  P        NQ+    P  G G    + +++F  + 
Sbjct: 241 NGAWNEGLA---QYDASSSLSLSMPSQNSPNVNNQSGLPEPRFGLGGGRAVTASAFPSTR 300

Query: 301 VAENSDSSQRNYRIRISSSNAQDSFASVGTVVRHPGGPSSQPPARLLPAEHVLDLRPAAA 360
             E      R         +   SF   G+ VR       Q PA   P    LD R    
Sbjct: 301 STETISRPGRRLNPGQPPESVAFSFTQSGSSVRQ----QQQLPA-TSPFVDPLDARAIPV 360

Query: 361 V-DNLSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSSSSAGERQVVQREEVRPNNMG 420
              + S  GQP MIH+PAL+R++  + W  SS+SR+ +          +  E + P +  
Sbjct: 361 TGSSSSGDGQPSMIHLPALTRNIHQFAWSASSSSRANS----------MPEEGLSPWDAP 420

Query: 421 RNVSEHPMF-VPANELRNIVQNQSSRGLTSSSLPIPGNVASTSRVGSSSGVH--PSSGPW 480
           R  SE P+F  PANE RN VQ+Q     T  +    G+     R GSSSG+H    +  W
Sbjct: 421 RINSEQPVFTTPANETRNPVQDQFCWSFTRGNPSTSGDSPFVPRAGSSSGIHGLQPNPTW 480

Query: 481 IPPESSPTQFPRRLTEYVRRQLFSSATNESG--GRINNYSQRSGSTLAQDMVLSSGSDRR 540
           + P +       R++E     LF S  +ES   G          S  + +    SGS  R
Sbjct: 481 VTPHNQ-----SRISEVAPWSLFPSIESESATHGASLPLLPTGPSVSSNEAAAPSGSSSR 540

Query: 541 GHHLLQPRSALWMERRGDGGLGLPYSLRTLATSNEGSDNNRLVSEIRNVLGLVRRGESLR 600
            H   Q RS L +ER+ D  L L +  R+LA  N+G   NRL+SEIR VL  +RRGE+LR
Sbjct: 541 SHRSRQRRSGLLLERQND-HLHLRHLGRSLAADNDG--RNRLISEIRQVLSAMRRGENLR 600

Query: 601 VEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLVLFILQYSFSSPQIEIKLSGALLLGTGP 660
            E                                                          
Sbjct: 601 FE---------------------------------------------------------- 660

Query: 661 CVYVIMPPWLETSFGALRYLYMDVMILDQSLFFGMADMYDRHRDMRLDVDNMSYEVGLMQ 720
                                 D M+ D  ++ GMA+M+DRHRDMRLDVDNMSYE     
Sbjct: 661 ----------------------DYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYE----- 666

Query: 721 TNQLKPLFRTFFLLELLALEERIGNVNTGLSEETIVGRLKQKKHV-NAVESQVEEEPCCV 780
                         ELLAL ERIG+V+TGLSEE I+  +KQ KH  +A  S  + EPCCV
Sbjct: 721 --------------ELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCV 666

Query: 781 CQEEYVEGDDMGTLECGHDFHTDCIKQWLMQKNLCPICKTTGIAS 808
           CQEEY EGDD+GTL CGH+FHT C+KQWLM KNLCPICKT  +++
Sbjct: 781 CQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVALST 666

BLAST of CmoCh05G001710 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 4.4e-62
Identity = 258/853 (30.25%), Postives = 363/853 (42.56%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVY-----WNNMWNPAENRIPDYLLPTSDVN 60
           MQG RS  GS +E          N    + +Y      N M NPA+   P+   P+    
Sbjct: 4   MQGPRSIGGSSTEV---------NQVDGESIYCTETSLNTMLNPADTGFPNNSTPSG--R 63

Query: 61  SGYVSSVSHEQRSLSRWSLGEPSSCDMQTDVL------------PDEQKAELGWSSMTRD 120
             Y SS SH  +  + W  GE SS    +D +             D     +G+ S  R 
Sbjct: 64  PTYASSSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQ 123

Query: 121 ADGPFTENQLCEPSNNPSLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEG 180
                  N +       +  H+   P  ++ S+SN +P  ++++        DS   N  
Sbjct: 124 TG----LNGMMVDGGVHAGSHIRNVPSFLRGSSSNPMPQHVDMSMDM-----DSDNCNAQ 183

Query: 181 TNVYKSSGSEEGGILCSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGN 240
           T+      +  G  L SS        +G S     +  G  GSS       CKRK++EG+
Sbjct: 184 TSGVVIRHNSYGSSLGSSVQ-----AAGESSSGPASPFGGWGSS-------CKRKALEGS 243

Query: 241 VAQSSLSGSSSYSQHIERGAEPPLPALPGRYNTGSRLSIPAP----------LARMNQAP 300
            +      + +     E  A     A   +Y   S LS+  P            R  Q  
Sbjct: 244 PSHYFSGETPNRIVQTENSAS---HASLSQYGASSSLSLATPSQSSPNVTNHFGRTEQMF 303

Query: 301 IRGTGELMSNSFSESIVAENSDSSQRNYRIRISSSNAQDSFA-SV---GTVVRHPGGPSS 360
             G G  ++ S   S    N+D+  R  R R++    Q+S A SV   GT VR  G    
Sbjct: 304 GSGGGRAVAASAFHS--TRNTDTLSRAGR-RLNPRQPQESVAFSVSHGGTSVRPTGSLQQ 363

Query: 361 QPPARLLPAEHVLDLRPAAAVD-NLSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSS 420
             P    P     D+R ++    + + + Q  ++H+PAL+R++  Y W  S +SR+ N S
Sbjct: 364 NLPLN-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRASNPS 423

Query: 421 --SSAGERQVVQREEVRPNNMGRNVSEHPMFVPANELRNIVQN-----QSSRGLTSSSLP 480
                 ER   Q E  R N       E P+F PA ++R  V +     + S G +  SL 
Sbjct: 424 GIGMPAERLGPQWETPRSN------QEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLF 483

Query: 481 IPGNVASTSRVGSSSGVH-PSSGP-WIPPESSPTQFPRRLTEYVRRQLFSSATNES---G 540
           +P       R G SS +H P   P WIPP+++P   P R +E     LF S  + S   G
Sbjct: 484 VP-------RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHG 543

Query: 541 GRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRGDGGLGLPYSLRTLATS 600
           G +        S  + ++ + S S+ R H     RS L +ER+ +  L L +  R+LA  
Sbjct: 544 GPL-PLLPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNE-LLHLRHIGRSLAA- 603

Query: 601 NEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLV 660
            +G+  N+++SEIR VL  +RRGE+LRVE                               
Sbjct: 604 -DGNGRNQIISEIRQVLHAMRRGENLRVE------------------------------- 663

Query: 661 LFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGALRYLYMDVMILDQSLFF 720
                                                            D M+ D  ++ 
Sbjct: 664 -------------------------------------------------DYMVFDPLIYQ 701

Query: 721 GMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELLALEERIGNVNTGLSEE 780
           GM DM+DRHR+MRLDVDNMSYE                   ELLAL ERIG+V+TGLSEE
Sbjct: 724 GMTDMHDRHREMRLDVDNMSYE-------------------ELLALGERIGDVSTGLSEE 701

Query: 781 TIVGRLKQKKHVNAVESQVE----EEPCCVCQEEYVEGDDMGTLECGHDFHTDCIKQWLM 806
            I+  +KQ KH ++  S VE     EPCC+CQEEYVEGD++GTL+CGH+FH DCIKQW+M
Sbjct: 784 VILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVM 701

BLAST of CmoCh05G001710 vs. TAIR 10
Match: AT2G15530.2 (RING/U-box superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 4.4e-62
Identity = 258/853 (30.25%), Postives = 363/853 (42.56%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGNQPVY-----WNNMWNPAENRIPDYLLPTSDVN 60
           MQG RS  GS +E          N    + +Y      N M NPA+   P+   P+    
Sbjct: 4   MQGPRSIGGSSTEV---------NQVDGESIYCTETSLNTMLNPADTGFPNNSTPSG--R 63

Query: 61  SGYVSSVSHEQRSLSRWSLGEPSSCDMQTDVL------------PDEQKAELGWSSMTRD 120
             Y SS SH  +  + W  GE SS    +D +             D     +G+ S  R 
Sbjct: 64  PTYASSSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQ 123

Query: 121 ADGPFTENQLCEPSNNPSLGHVNLSPLIIQNSNSNSIPHTLNLNASFASHGGDSSRVNEG 180
                  N +       +  H+   P  ++ S+SN +P  ++++        DS   N  
Sbjct: 124 TG----LNGMMVDGGVHAGSHIRNVPSFLRGSSSNPMPQHVDMSMDM-----DSDNCNAQ 183

Query: 181 TNVYKSSGSEEGGILCSSASDPLLLPSGSSGLPMVANDGRAGSSLEGRRAPCKRKSIEGN 240
           T+      +  G  L SS        +G S     +  G  GSS       CKRK++EG+
Sbjct: 184 TSGVVIRHNSYGSSLGSSVQ-----AAGESSSGPASPFGGWGSS-------CKRKALEGS 243

Query: 241 VAQSSLSGSSSYSQHIERGAEPPLPALPGRYNTGSRLSIPAP----------LARMNQAP 300
            +      + +     E  A     A   +Y   S LS+  P            R  Q  
Sbjct: 244 PSHYFSGETPNRIVQTENSAS---HASLSQYGASSSLSLATPSQSSPNVTNHFGRTEQMF 303

Query: 301 IRGTGELMSNSFSESIVAENSDSSQRNYRIRISSSNAQDSFA-SV---GTVVRHPGGPSS 360
             G G  ++ S   S    N+D+  R  R R++    Q+S A SV   GT VR  G    
Sbjct: 304 GSGGGRAVAASAFHS--TRNTDTLSRAGR-RLNPRQPQESVAFSVSHGGTSVRPTGSLQQ 363

Query: 361 QPPARLLPAEHVLDLRPAAAVD-NLSAQGQPIMIHVPALSRSLQPYRWIRSSTSRSGNSS 420
             P    P     D+R ++    + + + Q  ++H+PAL+R++  Y W  S +SR+ N S
Sbjct: 364 NLPLN-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRASNPS 423

Query: 421 --SSAGERQVVQREEVRPNNMGRNVSEHPMFVPANELRNIVQN-----QSSRGLTSSSLP 480
                 ER   Q E  R N       E P+F PA ++R  V +     + S G +  SL 
Sbjct: 424 GIGMPAERLGPQWETPRSN------QEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLF 483

Query: 481 IPGNVASTSRVGSSSGVH-PSSGP-WIPPESSPTQFPRRLTEYVRRQLFSSATNES---G 540
           +P       R G SS +H P   P WIPP+++P   P R +E     LF S  + S   G
Sbjct: 484 VP-------RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHG 543

Query: 541 GRINNYSQRSGSTLAQDMVLSSGSDRRGHHLLQPRSALWMERRGDGGLGLPYSLRTLATS 600
           G +        S  + ++ + S S+ R H     RS L +ER+ +  L L +  R+LA  
Sbjct: 544 GPL-PLLPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNE-LLHLRHIGRSLAA- 603

Query: 601 NEGSDNNRLVSEIRNVLGLVRRGESLRVEVGSLLVMYHGQVVHWLIYMLQCENGGEQQLV 660
            +G+  N+++SEIR VL  +RRGE+LRVE                               
Sbjct: 604 -DGNGRNQIISEIRQVLHAMRRGENLRVE------------------------------- 663

Query: 661 LFILQYSFSSPQIEIKLSGALLLGTGPCVYVIMPPWLETSFGALRYLYMDVMILDQSLFF 720
                                                            D M+ D  ++ 
Sbjct: 664 -------------------------------------------------DYMVFDPLIYQ 701

Query: 721 GMADMYDRHRDMRLDVDNMSYEVGLMQTNQLKPLFRTFFLLELLALEERIGNVNTGLSEE 780
           GM DM+DRHR+MRLDVDNMSYE                   ELLAL ERIG+V+TGLSEE
Sbjct: 724 GMTDMHDRHREMRLDVDNMSYE-------------------ELLALGERIGDVSTGLSEE 701

Query: 781 TIVGRLKQKKHVNAVESQVE----EEPCCVCQEEYVEGDDMGTLECGHDFHTDCIKQWLM 806
            I+  +KQ KH ++  S VE     EPCC+CQEEYVEGD++GTL+CGH+FH DCIKQW+M
Sbjct: 784 VILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVM 701

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMM43.7e-9034.18Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
O495009.9e-6732.00E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q7XTV72.9e-6631.19Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9ZQF96.2e-6130.25E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q5QLR52.8e-2946.26Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1HF130.0e+0087.76probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita moschata OX=3... [more]
A0A6J1HCX00.0e+0087.65probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1KBL50.0e+0086.16probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita maxima OX=366... [more]
A0A6J1K2J80.0e+0086.05probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A1S4DVI90.0e+0078.08probable E3 ubiquitin-protein ligase HIP1 OS=Cucumis melo OX=3656 GN=LOC10348787... [more]
Match NameE-valueIdentityDescription
XP_022962523.10.0e+0087.76probable E3 ubiquitin-protein ligase RHG1A isoform X2 [Cucurbita moschata][more]
KAG6598360.10.0e+0087.52putative E3 ubiquitin-protein ligase RHG1A, partial [Cucurbita argyrosperma subs... [more]
XP_022962521.10.0e+0087.65probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_0... [more]
KAG7029330.10.0e+0087.28putative E3 ubiquitin-protein ligase RHG1A [Cucurbita argyrosperma subsp. argyro... [more]
XP_023545718.10.0e+0086.90probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
AT5G42940.12.6e-9134.18RING/U-box superfamily protein [more]
AT1G45180.11.5e-8132.11RING/U-box superfamily protein [more]
AT4G34040.17.0e-6832.00RING/U-box superfamily protein [more]
AT2G15530.14.4e-6230.25RING/U-box superfamily protein [more]
AT2G15530.24.4e-6230.25RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 760..800
e-value: 1.3E-7
score: 41.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 760..801
e-value: 1.9E-10
score: 40.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 760..801
score: 12.386007
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 724..804
e-value: 2.8E-17
score: 64.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..173
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 192..251
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 430..474
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 379..395
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 221..239
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 430..461
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 379..414
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 1..575
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 656..805
NoneNo IPR availablePANTHERPTHR22937:SF139E3 UBIQUITIN-PROTEIN LIGASE MBR2-LIKE ISOFORM X1coord: 1..575
coord: 656..805
NoneNo IPR availableCDDcd16481RING-H2_TTC3coord: 759..801
e-value: 9.81936E-18
score: 75.0761
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 754..803

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh05G001710.1CmoCh05G001710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding