CmoCh04G031400 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G031400
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCmo_Chr04: 21749884 .. 21750891 (-)
RNA-Seq ExpressionCmoCh04G031400
SyntenyCmoCh04G031400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTCCACTGGCGACACTCAATCTCCTTCCTCATCTCCCAACCTTCCATCCAAGCGTAGGCTTGCCGATCTATTATCTTCCGTCCTTTTCCGACATGCTTTCCGATCAGATTTTACCGTCAACCGCGGTTTCCTCCGCCTACTCGACCGTAAAGTACCTCCTTCTTCCACCACTCGCGGCGTCGCCTCCTTCGACACCACACTCGATTCTTCATCCTCCGACCTCTGGCTTCGTGTTTATAATCCTCTCACATGTTCCGAGTCCGATCGCATGCCTGTTATCATATACTTCCACGGTGGAGGCTTTGTATTCGGCTCCGCCGACTCTCAGAAGATCGACTCGTTCTCTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCGTCTCCGTCAACTACCGCCTCGCGCCGGAGCACCGATTTCCGAGTCCATACGACGACGGATTTCGCGTTCTGAAGGCAATCGACGAAGGATCGATCTCCAAAAGTCTTCCAGAAAATGCTGATATAAGCAGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGAAACATCGTGCATCACGTGACGGTGAGAGCGGCGGAATATGAGTTCAAGAGGCTTAAGATCGCAGGTATGATTGCCATACAGCCATTTTTGGGAGGAGAAGAGCGGACAAAATCAGAGATTCGATTTGGGAAAGGCGGTGGACTGACTTTGGAAATGACGGATTGGTATTGGAAGGCGTTTCTACCGATCGGTTCGAGCCGGGATCACCCGGGCGCTAACGTGGTTCGGTCCGATGTTTCGGGGCTGAAATTACCACCGGCGCTTGTTGTGAATGGGGGCTTGGATTTGTTAAGCGATCGGATCAAAGAGTACTGTGAGTGGCTGGAGAAATCGGGTAAAGAGGTTCGGGTTGTGGAGTATCCGAATGGGATTCATGGGTTTAGTTGGTTTTCAGATCTTCCGGAATATTCCATGCTGATGCAAGACGCCAGAGAATTCACCAGAAGGGTCTTGTTAAATTAA

mRNA sequence

ATGCCTTCCACTGGCGACACTCAATCTCCTTCCTCATCTCCCAACCTTCCATCCAAGCGTAGGCTTGCCGATCTATTATCTTCCGTCCTTTTCCGACATGCTTTCCGATCAGATTTTACCGTCAACCGCGGTTTCCTCCGCCTACTCGACCGTAAAGTACCTCCTTCTTCCACCACTCGCGGCGTCGCCTCCTTCGACACCACACTCGATTCTTCATCCTCCGACCTCTGGCTTCGTGTTTATAATCCTCTCACATGTTCCGAGTCCGATCGCATGCCTGTTATCATATACTTCCACGGTGGAGGCTTTGTATTCGGCTCCGCCGACTCTCAGAAGATCGACTCGTTCTCTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCGTCTCCGTCAACTACCGCCTCGCGCCGGAGCACCGATTTCCGAGTCCATACGACGACGGATTTCGCGTTCTGAAGGCAATCGACGAAGGATCGATCTCCAAAAGTCTTCCAGAAAATGCTGATATAAGCAGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGAAACATCGTGCATCACGTGACGGTGAGAGCGGCGGAATATGAGTTCAAGAGGCTTAAGATCGCAGGTATGATTGCCATACAGCCATTTTTGGGAGGAGAAGAGCGGACAAAATCAGAGATTCGATTTGGGAAAGGCGGTGGACTGACTTTGGAAATGACGGATTGGTATTGGAAGGCGTTTCTACCGATCGGTTCGAGCCGGGATCACCCGGGCGCTAACGTGGTTCGGTCCGATGTTTCGGGGCTGAAATTACCACCGGCGCTTGTTGTGAATGGGGGCTTGGATTTGTTAAGCGATCGGATCAAAGAGTACTGTGAGTGGCTGGAGAAATCGGGTAAAGAGGTTCGGGTTGTGGAGTATCCGAATGGGATTCATGGGTTTAGTTGGTTTTCAGATCTTCCGGAATATTCCATGCTGATGCAAGACGCCAGAGAATTCACCAGAAGGGTCTTGTTAAATTAA

Coding sequence (CDS)

ATGCCTTCCACTGGCGACACTCAATCTCCTTCCTCATCTCCCAACCTTCCATCCAAGCGTAGGCTTGCCGATCTATTATCTTCCGTCCTTTTCCGACATGCTTTCCGATCAGATTTTACCGTCAACCGCGGTTTCCTCCGCCTACTCGACCGTAAAGTACCTCCTTCTTCCACCACTCGCGGCGTCGCCTCCTTCGACACCACACTCGATTCTTCATCCTCCGACCTCTGGCTTCGTGTTTATAATCCTCTCACATGTTCCGAGTCCGATCGCATGCCTGTTATCATATACTTCCACGGTGGAGGCTTTGTATTCGGCTCCGCCGACTCTCAGAAGATCGACTCGTTCTCTCGCGATTTTGCTCGCGAAATCGGAGCCATTGTCGTCTCCGTCAACTACCGCCTCGCGCCGGAGCACCGATTTCCGAGTCCATACGACGACGGATTTCGCGTTCTGAAGGCAATCGACGAAGGATCGATCTCCAAAAGTCTTCCAGAAAATGCTGATATAAGCAGCTGTTTCATCGCCGGCGAAAGCGCCGGAGGAAACATCGTGCATCACGTGACGGTGAGAGCGGCGGAATATGAGTTCAAGAGGCTTAAGATCGCAGGTATGATTGCCATACAGCCATTTTTGGGAGGAGAAGAGCGGACAAAATCAGAGATTCGATTTGGGAAAGGCGGTGGACTGACTTTGGAAATGACGGATTGGTATTGGAAGGCGTTTCTACCGATCGGTTCGAGCCGGGATCACCCGGGCGCTAACGTGGTTCGGTCCGATGTTTCGGGGCTGAAATTACCACCGGCGCTTGTTGTGAATGGGGGCTTGGATTTGTTAAGCGATCGGATCAAAGAGTACTGTGAGTGGCTGGAGAAATCGGGTAAAGAGGTTCGGGTTGTGGAGTATCCGAATGGGATTCATGGGTTTAGTTGGTTTTCAGATCTTCCGGAATATTCCATGCTGATGCAAGACGCCAGAGAATTCACCAGAAGGGTCTTGTTAAATTAA

Protein sequence

MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTRGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWKAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Homology
BLAST of CmoCh04G031400 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.5e-72
Identity = 145/327 (44.34%), Postives = 197/327 (60.24%), Query Frame = 0

Query: 10  PSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTRGVASFDTTL 69
           P+    LP K R+A  + S +  +A R D T+NR FLRL D + PP+     + S    +
Sbjct: 7   PNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFV 66

Query: 70  DSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDFAREIGAIVV 129
              S DLW R+Y P      D++PV+++FHGGGF F S ++   D+  R FAR++ A V+
Sbjct: 67  VDQSRDLWFRLYTPHV--SGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVI 126

Query: 130 SVNYRLAPEHRFPSPYDDGFRVLKAIDE--GSISKSLPENADISSCFIAGESAGGNIVHH 189
           SVNYRLAPEHR+P+ YDDGF  LK I+E  GSI   LP NAD+S CF AG+SAGGNI H+
Sbjct: 127 SVNYRLAPEHRYPAQYDDGFDALKYIEENHGSI---LPANADLSRCFFAGDSAGGNIAHN 186

Query: 190 VTVRAA---EYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWKAFLP 249
           V +R        F  +K+ G+I+IQPF GGEERT++E +      ++ + TDW WKA   
Sbjct: 187 VAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA--- 246

Query: 250 IGSSRDHPGANV---VRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVE 309
           +G +RDH   NV      D+SGL  P  +VV  G D L D  + Y EWL+  GK+  ++E
Sbjct: 247 MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIE 306

Query: 310 YPNGIHGFSWFSDLPEYSMLMQDAREF 329
           YPN  H F  F +LPE   L+   ++F
Sbjct: 307 YPNMFHAFYIFPELPEAGQLIMRIKDF 325

BLAST of CmoCh04G031400 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 4.4e-53
Identity = 121/309 (39.16%), Postives = 168/309 (54.37%), Query Frame = 0

Query: 36  RSDFTVNRGFLRLLDRKVPPSST-TRGVASFDTTLDSSSSDLWLRVYNPLTCSES----- 95
           R D T NR     LDRKVP ++    GV SFD  +D   ++L  RVY P     S     
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIID-RQTNLLSRVYRPADAGTSPSITD 95

Query: 96  -------DRMPVIIYFHGGGFVFGSADSQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFP 155
                  + +PVI++FHGG F   SA+S   D+  R      GA+VVSVNYR APE+R+P
Sbjct: 96  LQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYP 155

Query: 156 SPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESAGGNIVHHVTVRAAEYEFKRLKI 215
             YDDG+ VLK ++  S  +S  ++      F+AG+S+GGNIVH+V VRA E    R+ +
Sbjct: 156 CAYDDGWAVLKWVNSSSWLRSKKDSK--VRIFLAGDSSGGNIVHNVAVRAVE---SRIDV 215

Query: 216 AGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWKAFLPIGSSRDHPGANVV---RS 275
            G I + P  GG ERT+SE R      +T+   DWYW+AFLP G  R+HP  +       
Sbjct: 216 LGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSK 275

Query: 276 DVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYS 329
            + GL  P +LVV  GLDL+ D   +Y E L+K+G+EV+++       GF    +   + 
Sbjct: 276 SLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFH 335

BLAST of CmoCh04G031400 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.7e-49
Identity = 117/318 (36.79%), Postives = 165/318 (51.89%), Query Frame = 0

Query: 36  RSDFTVNRGFLRLLDRKVPPSS-TTRGVASFDTTLDSSSSDLWLRVYNPLTCSESDR--- 95
           R+D T  R     LDR+VP ++    GV+SFD  +D S   L +R+Y      +++    
Sbjct: 36  RADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVG-LEVRIYRAAAEGDAEEGAA 95

Query: 96  ------------------MPVIIYFHGGGFVFGSADSQKIDSFSRDFAREIGAIVVSVNY 155
                              PVII+FHGG FV  SA S   DS  R F +    +VVSVNY
Sbjct: 96  AVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNY 155

Query: 156 RLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESAGGNIVHHVTVRAA 215
           R APEHR+P  YDDG+  LK +      +S  +    +  F++G+S+GGNI HHV VRAA
Sbjct: 156 RRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQ--ARVFLSGDSSGGNIAHHVAVRAA 215

Query: 216 EYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWKAFLPIGSSRDHPG 275
           +   + +K+ G I +    GG ERT+SE R      +TL+  DWYWKA+LP  + RDHP 
Sbjct: 216 D---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDADRDHPA 275

Query: 276 ANVVRSD---VSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVEYPNGIHGFS 329
            N    +   + GL    +L++  GLDL  DR   Y + L + G  V+VV+  N   GF 
Sbjct: 276 CNPFGPNGRRLGGLPFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVGFY 335

BLAST of CmoCh04G031400 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.6e-49
Identity = 119/344 (34.59%), Postives = 183/344 (53.20%), Query Frame = 0

Query: 15  NLPSKRRLADLLSSVL---FRHAF----RSDFTVNRGFLRLLDRKVPPSS-TTRGVASFD 74
           NL   +R+  L + VL   F+ A+    R D + NR     LDRKVP +S    GV SFD
Sbjct: 8   NLNECKRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD 67

Query: 75  TTLDSSSSDLWLRVYNPLT---------------CSESDRMPVIIYFHGGGFVFGSADSQ 134
                S+++L  R+Y P +                S ++ +PV+I+FHGG F   SA+S 
Sbjct: 68  HV--DSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSA 127

Query: 135 KIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADIS 194
             D+F R      G +VVSV+YR +PEHR+P  YDDG+  L  + +  +     +++++ 
Sbjct: 128 IYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWV-KSRVWLQSGKDSNV- 187

Query: 195 SCFIAGESAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLT 254
             ++AG+S+GGNI H+V VRA     + +K+ G I + P  GG+ERT+SE        +T
Sbjct: 188 YVYLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVT 247

Query: 255 LEMTDWYWKAFLPIGSSRDHPGANVV---RSDVSGLKLPPALVVNGGLDLLSDRIKEYCE 314
           ++  DWYW+A+LP G  RDHP  N        + G+  P +LVV  GLDL+ D    Y +
Sbjct: 248 IQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVD 307

Query: 315 WLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 333
            L+K+G EV ++       GF +  +   +  LM++  +F   +
Sbjct: 308 GLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

BLAST of CmoCh04G031400 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.2e-46
Identity = 122/343 (35.57%), Postives = 170/343 (49.56%), Query Frame = 0

Query: 11  SSSPNLPSKRRLADLLSSVL---FRHAF----RSDFTVNRGFLRLLDRKVPPSST-TRGV 70
           S   NL   R +  L + VL   F+ A+    R D T NR     LDRKV  ++    GV
Sbjct: 4   SDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGV 63

Query: 71  ASFDTTLDSSSSDLWLRVYNPLTCS--------------ESDRMPVIIYFHGGGFVFGSA 130
            SFD  +D    +L  RVY P                  + D +PVI++FHGG F   SA
Sbjct: 64  FSFDVLID-RRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSA 123

Query: 131 DSQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENA 190
           +S   D+  R        +VVSVNYR APE+ +P  YDDG+  L  ++  S  KS  ++ 
Sbjct: 124 NSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSK 183

Query: 191 DISSCFIAGESAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGG 250
                F+AG+S+GGNI H+V +RA E     + + G I + P  GG ERT+SE       
Sbjct: 184 --VHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKY 243

Query: 251 GLTLEMTDWYWKAFLPIGSSRDHPGANVVR---SDVSGLKLPPALVVNGGLDLLSDRIKE 310
            +T+   DWYWKAFLP G  R+HP  N        + G+  P +LVV  GLDL+ D    
Sbjct: 244 FVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLA 303

Query: 311 YCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 329
           Y E L+K+G+EV+++       GF    +   +  +M +   F
Sbjct: 304 YAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAF 340

BLAST of CmoCh04G031400 vs. ExPASy TrEMBL
Match: A0A6J1HPS2 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 3.8e-193
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60
           MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR
Sbjct: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180

Query: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240
           GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240

Query: 241 AFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 300
           AFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV
Sbjct: 241 AFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 300

Query: 301 EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336
           EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 335

BLAST of CmoCh04G031400 vs. ExPASy TrEMBL
Match: A0A6J1JQL4 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 9.8e-181
Identity = 317/337 (94.07%), Postives = 325/337 (96.44%), Query Frame = 0

Query: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60
           M STGDTQSPSSSPNLPSKR+LADL +SVLFRH FRSDFTVNRG LRLLDRKVPP STTR
Sbjct: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPP-STTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNP TCSESDRMPVIIYFHGGGFVFGSADSQ+IDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSI+KSLPENADIS CFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESA 180

Query: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240
           GGNIVHHVTVRAA+YEFKRL+IAGMIAIQPFLGGEERT+SEIRFG+GGGLTLE  DWYWK
Sbjct: 181 GGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWK 240

Query: 241 AFLPIGSSRDHPGANVVR--SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           AFLPIGSSRDHP ANVVR  SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR
Sbjct: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336
           VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336

BLAST of CmoCh04G031400 vs. ExPASy TrEMBL
Match: A0A0A0LMQ6 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G401430 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.6e-138
Identity = 244/333 (73.27%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MPSTGDTQSPSSS-PNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTT 60
           M STGD QSP+SS P LP KR+LADL +S LFR AFRSDFTVNR FLRL DRK+PP  T+
Sbjct: 1   MASTGDAQSPNSSLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPP-FTS 60

Query: 61  RGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRD 120
           RGVA+ D T+DSS+SDLW+RVYNPLT S SD +PVIIYFHGGGFV+GSAD+   D+F RD
Sbjct: 61  RGVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYGSADAPPTDTFCRD 120

Query: 121 FAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGES 180
           FAREIGAIV+SVNYRLAPE RFPS +DDGF VLKA+D+G+IS+++PENAD+  CFIAGES
Sbjct: 121 FAREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGES 180

Query: 181 AGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYW 240
           AGGNI HHVTVRAAE EFKR+KI GMI IQPF GGEER  SEIRFG+G GLTLEMTDW+W
Sbjct: 181 AGGNIAHHVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFGRGYGLTLEMTDWFW 240

Query: 241 KAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV 300
           KA+LP+GS+RDH  ANVV S +SG+K+P ALVV GGLDLL DR +EY EWL+KSG+EVRV
Sbjct: 241 KAWLPVGSNRDHTAANVVGSSISGVKVPAALVVIGGLDLLRDRNREYVEWLKKSGQEVRV 300

Query: 301 VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 333
           VEYPNG HGF    DLPEYSML+QDA++F  ++
Sbjct: 301 VEYPNGTHGFIGKPDLPEYSMLIQDAKQFINKI 332

BLAST of CmoCh04G031400 vs. ExPASy TrEMBL
Match: A0A5A7T4I6 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00840 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.7e-137
Identity = 246/327 (75.23%), Postives = 276/327 (84.40%), Query Frame = 0

Query: 1   MPSTGDTQSPSSS-PNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTT 60
           M S GD QSP+SS P LP  R+LADL +S LFR AFRSDFTVNR  L LLDRKVPP  TT
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPP-FTT 60

Query: 61  RGVASFDTTLDSSSSD-LWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSR 120
           RGVA+ D  +DSSSSD LW+RVYNPLT   SD +PVI++FHGGGFV+GSADS  ID+F R
Sbjct: 61  RGVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFCR 120

Query: 121 DFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGE 180
           DFAREIGAIV+SVNYRLAPEHRFPS +DDGF VLKA++EG IS++LPENAD+  CFIAGE
Sbjct: 121 DFAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAGE 180

Query: 181 SAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWY 240
           SAGGNI HHVTVRAAE E KRLKI GMI IQPFLGGEER  SEIRFG+G G+T+EM DW+
Sbjct: 181 SAGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRFGRGYGMTVEMADWF 240

Query: 241 WKAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           WKA+LP+GS+RDHP ANVVRS +SG+KLPPA+VV GGLDLL DR KEY EWL+K G+EVR
Sbjct: 241 WKAWLPVGSNRDHPAANVVRSSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKWGEEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDA 326
           VVEYPNG HGF W  DLPEYSMLMQDA
Sbjct: 301 VVEYPNGTHGFIWKPDLPEYSMLMQDA 326

BLAST of CmoCh04G031400 vs. ExPASy TrEMBL
Match: A0A1S3B2W2 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.7e-137
Identity = 246/327 (75.23%), Postives = 276/327 (84.40%), Query Frame = 0

Query: 1   MPSTGDTQSPSSS-PNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTT 60
           M S GD QSP+SS P LP  R+LADL +S LFR AFRSDFTVNR  L LLDRKVPP  TT
Sbjct: 1   MASAGDAQSPNSSPPTLPWNRKLADLFTSFLFRRAFRSDFTVNRRLLGLLDRKVPP-FTT 60

Query: 61  RGVASFDTTLDSSSSD-LWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSR 120
           RGVA+ D  +DSSSSD LW+RVYNPLT   SD +PVI++FHGGGFV+GSADS  ID+F R
Sbjct: 61  RGVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVYGSADSPPIDTFCR 120

Query: 121 DFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGE 180
           DFAREIGAIV+SVNYRLAPEHRFPS +DDGF VLKA++EG IS++LPENAD+  CFIAGE
Sbjct: 121 DFAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVISETLPENADLRRCFIAGE 180

Query: 181 SAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWY 240
           SAGGNI HHVTVRAAE E KRLKI GMI IQPFLGGEER  SEIRFG+G G+T+EM DW+
Sbjct: 181 SAGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRFGRGYGMTVEMADWF 240

Query: 241 WKAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           WKA+LP+GS+RDHP ANVVRS +SG+KLPPA+VV GGLDLL DR KEY EWL+K G+EVR
Sbjct: 241 WKAWLPVGSNRDHPAANVVRSSISGVKLPPAVVVIGGLDLLRDRQKEYVEWLKKWGEEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDA 326
           VVEYPNG HGF W  DLPEYSMLMQDA
Sbjct: 301 VVEYPNGTHGFIWKPDLPEYSMLMQDA 326

BLAST of CmoCh04G031400 vs. NCBI nr
Match: XP_022964989.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 683.7 bits (1763), Expect = 7.9e-193
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0

Query: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60
           MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR
Sbjct: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180

Query: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240
           GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240

Query: 241 AFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 300
           AFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV
Sbjct: 241 AFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 300

Query: 301 EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336
           EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 335

BLAST of CmoCh04G031400 vs. NCBI nr
Match: KAG6602724.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033410.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 672.9 bits (1735), Expect = 1.4e-189
Identity = 328/335 (97.91%), Postives = 333/335 (99.40%), Query Frame = 0

Query: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60
           MPSTGDTQSPSSSPNLPSKR+LADLLSS+LFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR
Sbjct: 1   MPSTGDTQSPSSSPNLPSKRKLADLLSSILFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQ+IDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180
           AREIGAIVVSVNY LAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA
Sbjct: 121 AREIGAIVVSVNYCLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180

Query: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240
           GGNIVHHVTVRAAEYEFKRL+IAGMIAIQPFLGGEERTKSEIRFG+GGGLTLEMTDWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLRIAGMIAIQPFLGGEERTKSEIRFGRGGGLTLEMTDWYWK 240

Query: 241 AFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 300
           AFLPIGSSRDHP ANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV
Sbjct: 241 AFLPIGSSRDHPAANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVV 300

Query: 301 EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336
           EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 EYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 335

BLAST of CmoCh04G031400 vs. NCBI nr
Match: XP_023549493.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649.0 bits (1673), Expect = 2.2e-182
Identity = 318/333 (95.50%), Postives = 326/333 (97.90%), Query Frame = 0

Query: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60
           M STGDTQSPSSSPNLPSKR+LADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSST R
Sbjct: 1   MSSTGDTQSPSSSPNLPSKRKLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTNR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNP TCSESDR+PVIIYFHGGGFVFGSADSQ+IDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRVPVIIYFHGGGFVFGSADSQQIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADIS CFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISRCFIAGESA 180

Query: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240
           GGNIVHHVTVRAAEYEFKRL+IAGMIAIQPFLGGEERTKSEIRFG+GGGLTLE+TDWYWK
Sbjct: 181 GGNIVHHVTVRAAEYEFKRLRIAGMIAIQPFLGGEERTKSEIRFGRGGGLTLEVTDWYWK 240

Query: 241 AFLPIGSSRDHPGANVVR--SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           AFLPIGSSRDHP ANVVR  SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLE+SGKEVR
Sbjct: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLERSGKEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRR 332
           VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTR+
Sbjct: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRQ 333

BLAST of CmoCh04G031400 vs. NCBI nr
Match: XP_022990625.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 642.5 bits (1656), Expect = 2.0e-180
Identity = 317/337 (94.07%), Postives = 325/337 (96.44%), Query Frame = 0

Query: 1   MPSTGDTQSPSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTR 60
           M STGDTQSPSSSPNLPSKR+LADL +SVLFRH FRSDFTVNRG LRLLDRKVPP STTR
Sbjct: 1   MSSTGDTQSPSSSPNLPSKRKLADLFTSVLFRHVFRSDFTVNRGLLRLLDRKVPP-STTR 60

Query: 61  GVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDF 120
           GVASFDTTLDSSSSDLWLRVYNP TCSESDRMPVIIYFHGGGFVFGSADSQ+IDSFSRDF
Sbjct: 61  GVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFGSADSQQIDSFSRDF 120

Query: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESA 180
           AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSI+KSLPENADIS CFIAGESA
Sbjct: 121 AREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIAKSLPENADISRCFIAGESA 180

Query: 181 GGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWK 240
           GGNIVHHVTVRAA+YEFKRL+IAGMIAIQPFLGGEERT+SEIRFG+GGGLTLE  DWYWK
Sbjct: 181 GGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFGRGGGLTLETADWYWK 240

Query: 241 AFLPIGSSRDHPGANVVR--SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300
           AFLPIGSSRDHP ANVVR  SDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR
Sbjct: 241 AFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVR 300

Query: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336
           VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN
Sbjct: 301 VVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRVLLN 336

BLAST of CmoCh04G031400 vs. NCBI nr
Match: XP_004138821.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN63083.1 hypothetical protein Csa_021962 [Cucumis sativus])

HSP 1 Score: 502.3 bits (1292), Expect = 3.3e-138
Identity = 244/333 (73.27%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 1   MPSTGDTQSPSSS-PNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTT 60
           M STGD QSP+SS P LP KR+LADL +S LFR AFRSDFTVNR FLRL DRK+PP  T+
Sbjct: 1   MASTGDAQSPNSSLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPP-FTS 60

Query: 61  RGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRD 120
           RGVA+ D T+DSS+SDLW+RVYNPLT S SD +PVIIYFHGGGFV+GSAD+   D+F RD
Sbjct: 61  RGVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYGSADAPPTDTFCRD 120

Query: 121 FAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGES 180
           FAREIGAIV+SVNYRLAPE RFPS +DDGF VLKA+D+G+IS+++PENAD+  CFIAGES
Sbjct: 121 FAREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGES 180

Query: 181 AGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYW 240
           AGGNI HHVTVRAAE EFKR+KI GMI IQPF GGEER  SEIRFG+G GLTLEMTDW+W
Sbjct: 181 AGGNIAHHVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFGRGYGLTLEMTDWFW 240

Query: 241 KAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRV 300
           KA+LP+GS+RDH  ANVV S +SG+K+P ALVV GGLDLL DR +EY EWL+KSG+EVRV
Sbjct: 241 KAWLPVGSNRDHTAANVVGSSISGVKVPAALVVIGGLDLLRDRNREYVEWLKKSGQEVRV 300

Query: 301 VEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 333
           VEYPNG HGF    DLPEYSML+QDA++F  ++
Sbjct: 301 VEYPNGTHGFIGKPDLPEYSMLIQDAKQFINKI 332

BLAST of CmoCh04G031400 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 272.7 bits (696), Expect = 3.9e-73
Identity = 145/327 (44.34%), Postives = 197/327 (60.24%), Query Frame = 0

Query: 10  PSSSPNLPSKRRLADLLSSVLFRHAFRSDFTVNRGFLRLLDRKVPPSSTTRGVASFDTTL 69
           P+    LP K R+A  + S +  +A R D T+NR FLRL D + PP+     + S    +
Sbjct: 7   PNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFV 66

Query: 70  DSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVFGSADSQKIDSFSRDFAREIGAIVV 129
              S DLW R+Y P      D++PV+++FHGGGF F S ++   D+  R FAR++ A V+
Sbjct: 67  VDQSRDLWFRLYTPHV--SGDKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVI 126

Query: 130 SVNYRLAPEHRFPSPYDDGFRVLKAIDE--GSISKSLPENADISSCFIAGESAGGNIVHH 189
           SVNYRLAPEHR+P+ YDDGF  LK I+E  GSI   LP NAD+S CF AG+SAGGNI H+
Sbjct: 127 SVNYRLAPEHRYPAQYDDGFDALKYIEENHGSI---LPANADLSRCFFAGDSAGGNIAHN 186

Query: 190 VTVRAA---EYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWKAFLP 249
           V +R        F  +K+ G+I+IQPF GGEERT++E +      ++ + TDW WKA   
Sbjct: 187 VAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA--- 246

Query: 250 IGSSRDHPGANV---VRSDVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVE 309
           +G +RDH   NV      D+SGL  P  +VV  G D L D  + Y EWL+  GK+  ++E
Sbjct: 247 MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIE 306

Query: 310 YPNGIHGFSWFSDLPEYSMLMQDAREF 329
           YPN  H F  F +LPE   L+   ++F
Sbjct: 307 YPNMFHAFYIFPELPEAGQLIMRIKDF 325

BLAST of CmoCh04G031400 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.1e-54
Identity = 121/309 (39.16%), Postives = 168/309 (54.37%), Query Frame = 0

Query: 36  RSDFTVNRGFLRLLDRKVPPSST-TRGVASFDTTLDSSSSDLWLRVYNPLTCSES----- 95
           R D T NR     LDRKVP ++    GV SFD  +D   ++L  RVY P     S     
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIID-RQTNLLSRVYRPADAGTSPSITD 95

Query: 96  -------DRMPVIIYFHGGGFVFGSADSQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFP 155
                  + +PVI++FHGG F   SA+S   D+  R      GA+VVSVNYR APE+R+P
Sbjct: 96  LQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYP 155

Query: 156 SPYDDGFRVLKAIDEGSISKSLPENADISSCFIAGESAGGNIVHHVTVRAAEYEFKRLKI 215
             YDDG+ VLK ++  S  +S  ++      F+AG+S+GGNIVH+V VRA E    R+ +
Sbjct: 156 CAYDDGWAVLKWVNSSSWLRSKKDSK--VRIFLAGDSSGGNIVHNVAVRAVE---SRIDV 215

Query: 216 AGMIAIQPFLGGEERTKSEIRFGKGGGLTLEMTDWYWKAFLPIGSSRDHPGANVV---RS 275
            G I + P  GG ERT+SE R      +T+   DWYW+AFLP G  R+HP  +       
Sbjct: 216 LGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSK 275

Query: 276 DVSGLKLPPALVVNGGLDLLSDRIKEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYS 329
            + GL  P +LVV  GLDL+ D   +Y E L+K+G+EV+++       GF    +   + 
Sbjct: 276 SLEGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFH 335

BLAST of CmoCh04G031400 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 196.1 bits (497), Expect = 4.7e-50
Identity = 119/344 (34.59%), Postives = 183/344 (53.20%), Query Frame = 0

Query: 15  NLPSKRRLADLLSSVL---FRHAF----RSDFTVNRGFLRLLDRKVPPSS-TTRGVASFD 74
           NL   +R+  L + VL   F+ A+    R D + NR     LDRKVP +S    GV SFD
Sbjct: 8   NLNECKRIVPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD 67

Query: 75  TTLDSSSSDLWLRVYNPLT---------------CSESDRMPVIIYFHGGGFVFGSADSQ 134
                S+++L  R+Y P +                S ++ +PV+I+FHGG F   SA+S 
Sbjct: 68  HV--DSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSA 127

Query: 135 KIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENADIS 194
             D+F R      G +VVSV+YR +PEHR+P  YDDG+  L  + +  +     +++++ 
Sbjct: 128 IYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWV-KSRVWLQSGKDSNV- 187

Query: 195 SCFIAGESAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGGGLT 254
             ++AG+S+GGNI H+V VRA     + +K+ G I + P  GG+ERT+SE        +T
Sbjct: 188 YVYLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVT 247

Query: 255 LEMTDWYWKAFLPIGSSRDHPGANVV---RSDVSGLKLPPALVVNGGLDLLSDRIKEYCE 314
           ++  DWYW+A+LP G  RDHP  N        + G+  P +LVV  GLDL+ D    Y +
Sbjct: 248 IQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVD 307

Query: 315 WLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 333
            L+K+G EV ++       GF +  +   +  LM++  +F   +
Sbjct: 308 GLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

BLAST of CmoCh04G031400 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.7e-47
Identity = 122/343 (35.57%), Postives = 170/343 (49.56%), Query Frame = 0

Query: 11  SSSPNLPSKRRLADLLSSVL---FRHAF----RSDFTVNRGFLRLLDRKVPPSST-TRGV 70
           S   NL   R +  L + VL   F+ A+    R D T NR     LDRKV  ++    GV
Sbjct: 4   SDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPVDGV 63

Query: 71  ASFDTTLDSSSSDLWLRVYNPLTCS--------------ESDRMPVIIYFHGGGFVFGSA 130
            SFD  +D    +L  RVY P                  + D +PVI++FHGG F   SA
Sbjct: 64  FSFDVLID-RRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHSSA 123

Query: 131 DSQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSISKSLPENA 190
           +S   D+  R        +VVSVNYR APE+ +P  YDDG+  L  ++  S  KS  ++ 
Sbjct: 124 NSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSRSWLKSKKDSK 183

Query: 191 DISSCFIAGESAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRFGKGG 250
                F+AG+S+GGNI H+V +RA E     + + G I + P  GG ERT+SE       
Sbjct: 184 --VHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKY 243

Query: 251 GLTLEMTDWYWKAFLPIGSSRDHPGANVVR---SDVSGLKLPPALVVNGGLDLLSDRIKE 310
            +T+   DWYWKAFLP G  R+HP  N        + G+  P +LVV  GLDL+ D    
Sbjct: 244 FVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQLA 303

Query: 311 YCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREF 329
           Y E L+K+G+EV+++       GF    +   +  +M +   F
Sbjct: 304 YAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAF 340

BLAST of CmoCh04G031400 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 178.7 bits (452), Expect = 7.7e-45
Identity = 108/269 (40.15%), Postives = 142/269 (52.79%), Query Frame = 0

Query: 53  VPPSSTTRGVASFDTTLD-SSSSDLWLRVYNP--LTCSESDRMPVIIYFHGGGFVFGSAD 112
           VP  S T   +S  T  D   S+D W RVY P     S S  +P+++YFHGGGF  GSA 
Sbjct: 50  VPIVSPTIHPSSKATAFDIKLSNDTWTRVYIPDAAAASPSVTLPLLVYFHGGGFCVGSAA 109

Query: 113 SQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVL-----KAIDEGSISKSL 172
                 F    A +   ++VSVNYRLAPEHR P+ YDDG  V+     + I  G    S 
Sbjct: 110 WSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYDDGVNVVSWLVKQQISTGGGYPSW 169

Query: 173 PENADISSCFIAGESAGGNIVHHVTVR--AAEYEFKRLKIAGMIAIQPFLGGEERTKSEI 232
               ++S+ F+AG+SAG NI + V VR  A+      L + G+I I PF GGE RT SE 
Sbjct: 170 LSKCNLSNVFLAGDSAGANIAYQVAVRIMASGKYANTLHLKGIILIHPFFGGESRTSSEK 229

Query: 233 R--FGKGGGLTLEMTDWYWKAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLS 292
           +    K   LTL  +D YW+  LP G+SRDHP  N + S  +G KLP  +V     D+L 
Sbjct: 230 QQHHTKSSALTLSASDAYWRLALPRGASRDHPWCNPLMSS-AGAKLPTTMVFMAEFDILK 289

Query: 293 DRIKEYCEWLEKSGKEVRVVEYPNGIHGF 310
           +R  E C+ +   GK V  + +    H F
Sbjct: 290 ERNLEMCKVMRSHGKRVEGIVHGGVGHAF 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT105.5e-7244.34Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G64.4e-5339.16Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5451.7e-4936.79Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9LYC16.6e-4934.59Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Q9MAA75.2e-4635.57Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HPS23.8e-193100.00probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 ... [more]
A0A6J1JQL49.8e-18194.07probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV... [more]
A0A0A0LMQ61.6e-13873.27Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G4014... [more]
A0A5A7T4I61.7e-13775.23Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B2W21.7e-13775.23probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022964989.17.9e-193100.00probable carboxylesterase 18 [Cucurbita moschata][more]
KAG6602724.11.4e-18997.91putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023549493.12.2e-18295.50probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
XP_022990625.12.0e-18094.07probable carboxylesterase 18 [Cucurbita maxima][more]
XP_004138821.13.3e-13873.27probable carboxylesterase 18 [Cucumis sativus] >KGN63083.1 hypothetical protein ... [more]
Match NameE-valueIdentityDescription
AT5G23530.13.9e-7344.34carboxyesterase 18 [more]
AT5G27320.13.1e-5439.16alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.14.7e-5034.59alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.13.7e-4735.57alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.17.7e-4540.15carboxyesterase 17 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 5..333
e-value: 2.4E-83
score: 282.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 58..333
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 95..309
e-value: 3.3E-59
score: 200.3
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 12..331
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 12..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G031400.1CmoCh04G031400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity