CmoCh04G027100 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G027100
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRNA pseudouridine synthase 5
LocationCmo_Chr04: 19683191 .. 19685960 (-)
RNA-Seq ExpressionCmoCh04G027100
SyntenyCmoCh04G027100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCAATACCTGAGGTTGCTCAGGGCCTGTACGTTGCTACTTCCTCAGGTTTTTGCTCGCATCTTATATGCTGATTCAATAAAACTCATGTTTTCTTGAGTGGACAGTGTTGCTACCATAAAAATGTACGTGATAGGGAAAGCAAAAGTCTCTTAAGTTGTTGGCTAAGAAAGTGGAGTGTCATTATTCATGGATTTCTAGATAAATTTTATATGGCCATCACGATCATGATCCTGTTCATGTTTACTTCCATTGTCTATCTGACCGTGTTTCAATAGTTACTAGCTTCAATATTCAATCATGTTTACTTTTTTTGAATTTATAACTTGAGCAGGGAAACCAGCTTTCAGAAAAGTCAATGTTCTTGAGATAGACGAACGAGGAGAACACACTTTGGTCCAGGTATCTTCTTTTGTTGGAAGAACTTCCTGACTACAGTTCTCTGTACATGCTTTTCTATTTAAAATGATTTTTCTGCTGTTATTCACATTTTTGTTAGTTTTACTGTCGCCAAAGTATATGAGTTTCTTTCACTTTTAAGTATGTCCTAAGCATGGGTATACCTTCCGCTAACCCTCACCACGCTTGTTCTGTGAAATACTTAAGGGGATGTACCTCTCCTTTTTATCATTTCTCCTCTACTGATTTCTTTGAAATAGGTCACAGAGGACTTCTTCTGTTTGTTGAGTTGCAATTACATGCCCAAGAGACTTCTGATTGGTAAAGAAAGAGGGAGGGCTTATTCCATTAGGCTTAAAAGGCACCTTATGTATTTACACGTACTGCAACGACCAAACTTTTATAATCTTCGCATTTTCATTTTCTGCTTGTACGTAAAACTACAATTTAATTTTCTGGTGCGATGGGCTTTACTGCCAAGGTCTAAATTCTTCACTATTTTCCAATTATTTTTGGAAGCCATTTAAAGAGAGTTTGCAGCTGGTCTGATAGTGGAAATATTCTTGATAAAGAAAGTGAACTGTGCGATGCACCACAATCCAAAGGGTTTAAATTTCGTCATATATTTTACAGGTGGAAATTCAATCTGGATGACCCCGCCAAATCCGTGTCCATCTTTCTTCAATAGGCATCCTCTATTGGGTAATGCTTCTTTAAAGTTGATCAATTCTTTCAACTTTTAAATTTTTTGGGGGTTCTCATTGCTTGTTTTGCAGATGAACTTTTTTCTAACAACCATTAGTTGACTTGCGTTTTTGTGAGCTCGCAATTTCATCGACTGTGCTGAAAATGCGTTGCTCAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAAAAAAAAAAAAAAAAAAACTGTCTTTTCCTGTTAGGTGATCCTCTCTATGTTGCTGTCCCCATCTTGAATTTGATTCCTCTTTCTTCTTGCAAAGAAACCATGCACATTCTGTCCAGAAGTTACCAAAAGGCTAAGAAACCTGTGCCTGGAGATTATAGCTACTACTTGCACGCACATCAATTGGTCCTTTATCACCCAAGAACCAATGAGGTAAGCTTTGAATTTAGTATATAACATAGTGTCATACTTCCGTTTTCTTGTCCAGATGCTTGTGTCCATTGAGAATAATAGAGATGCAAAAATTCAATGGTATGATGCATTTATATTTTTTGAACAACGCCCACGTTGGCAGTCAAGTAATATTATAGTAGGCTAGACTCTACTGGGTTATATATGCCTTCACATATGATGCAATTTCATTAGATAACTATTTGTATGCGTATGGGAAGACAATATTTTCATGGTTGGCGCGCATTGGTCTTGTCCAATCATCTCGTGTTAACCTATTCCTATGGAAGCATTCATGATATTTTATATGGCTTCCCCCTTTAAATAAACGTCTCAATTTGTTGAGTTGGCACATGAAGCAAGCATTTTATGTGAAGTGGCTTCCTAGTGCTTTCTTTCTTGTATTTGTTTTATACTTGTTTCTTTCATGTATGACTAAAAAGGGACTTGTCTCTTGTTCATGTTGAAAGGTTGTCAAAATCGTTGAGCCACTCCGGAAGGGGTCTGAAGATGCCAGAAATGGAGTGGATGAAAAAAGCATAGCATGGATGTCAAAAAGATTAGTCCTAATCTCTTATCCTTATACTTTAATATCACAAAGCATATCTTTTCGTTTACGTTTTTTTTTTTTTAATGAAAGATATTATAAAAGATTGGTTCTAGAAATAATTTAAAAGATAAGACCTATACATCCTCTTATAGGTCCCCATCAGATCTGCCAACCAGATGTGCTATTTAGGCATGGCACTATTAACCTCTTCTGCCTGAAACATTTAGGAATATCAAATTCCAAATGAACAGAAATCAAATGGCGATTCCCAACAGCTCCGTAAGTGTTGACTGGAATTGAAATTTATGTTAAACTGAAGAAAAGTAATTTTGAAGCATTACTCTTTTCTTTTTCCTCCTACAAAGACGGCAATAAGAACCGTCAAATTCTGCTATCATTCATTGACATCAACGAAACTATGATACATGGGGTGCAGCAAAAGAAACCAATTGCTGCTAAGAGAGTGCCTCTGTTGAGAGGCTCTCTCTAA

mRNA sequence

ATGGATGCAATACCTGAGGTTGCTCAGGGCCTGTACGTTGCTACTTCCTCAGGGAAACCAGCTTTCAGAAAAGTCAATGTTCTTGAGATAGACGAACGAGGAGAACACACTTTGGTCCAGGTCACAGAGGACTTCTTCTGTTTGTTGAGTTGCAATTACATGCCCAAGAGACTTCTGATTGGTGGAAATTCAATCTGGATGACCCCGCCAAATCCGTGTCCATCTTTCTTCAATAGGCATCCTCTATTGGAAACCATGCACATTCTGTCCAGAAGTTACCAAAAGGCTAAGAAACCTGTGCCTGGAGATTATAGCTACTACTTGCACGCACATCAATTGGTCCTTTATCACCCAAGAACCAATGAGGTTGTCAAAATCGTTGAGCCACTCCGGAAGGGGTCTGAAGATGCCAGAAATGGAGTGGATGAAAAAAGCATAGCATGGATGTCAAAAAGATTAGAATATCAAATTCCAAATGAACAGAAATCAAATGGCGATTCCCAACAGCTCCACGGCAATAAGAACCGTCAAATTCTGCTATCATTCATTGACATCAACGAAACTATGATACATGGGGTGCAGCAAAAGAAACCAATTGCTGCTAAGAGAGTGCCTCTGTTGAGAGGCTCTCTCTAA

Coding sequence (CDS)

ATGGATGCAATACCTGAGGTTGCTCAGGGCCTGTACGTTGCTACTTCCTCAGGGAAACCAGCTTTCAGAAAAGTCAATGTTCTTGAGATAGACGAACGAGGAGAACACACTTTGGTCCAGGTCACAGAGGACTTCTTCTGTTTGTTGAGTTGCAATTACATGCCCAAGAGACTTCTGATTGGTGGAAATTCAATCTGGATGACCCCGCCAAATCCGTGTCCATCTTTCTTCAATAGGCATCCTCTATTGGAAACCATGCACATTCTGTCCAGAAGTTACCAAAAGGCTAAGAAACCTGTGCCTGGAGATTATAGCTACTACTTGCACGCACATCAATTGGTCCTTTATCACCCAAGAACCAATGAGGTTGTCAAAATCGTTGAGCCACTCCGGAAGGGGTCTGAAGATGCCAGAAATGGAGTGGATGAAAAAAGCATAGCATGGATGTCAAAAAGATTAGAATATCAAATTCCAAATGAACAGAAATCAAATGGCGATTCCCAACAGCTCCACGGCAATAAGAACCGTCAAATTCTGCTATCATTCATTGACATCAACGAAACTATGATACATGGGGTGCAGCAAAAGAAACCAATTGCTGCTAAGAGAGTGCCTCTGTTGAGAGGCTCTCTCTAA

Protein sequence

MDAIPEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVTEDFFCLLSCNYMPKRLLIGGNSIWMTPPNPCPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVLYHPRTNEVVKIVEPLRKGSEDARNGVDEKSIAWMSKRLEYQIPNEQKSNGDSQQLHGNKNRQILLSFIDINETMIHGVQQKKPIAAKRVPLLRGSL
Homology
BLAST of CmoCh04G027100 vs. ExPASy Swiss-Prot
Match: Q0DST9 (RNA pseudouridine synthase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0288500 PE=2 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 3.3e-14
Identity = 54/137 (39.42%), Postives = 66/137 (48.18%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLL- 64
           P VA+GLY A SSGKPA  KV VLE  +   HTL+QV           +   Y+   L+ 
Sbjct: 252 PGVAEGLYAACSSGKPAMSKVRVLERLKIHNHTLIQVEIHSGRPHQIRIHLAYIGHPLVD 311

Query: 65  -----IGGNSIWMTPPNPCPSFFNRHPL-LETMHILSRSYQKAKKPVPGDYSYYLHAHQL 124
                IGG           P F    P            Y++  +PVPGD  Y+LHAH L
Sbjct: 312 DPLYGIGGQ----------PKFDEPEPTSTADSFAYDGGYERPLQPVPGDCGYHLHAHWL 371

Query: 125 VLYHPRTNEVVKIVEPL 131
           VL HP TNE++KI  PL
Sbjct: 372 VLCHPTTNEMIKITAPL 378

BLAST of CmoCh04G027100 vs. ExPASy Swiss-Prot
Match: Q5M721 (RNA pseudouridine synthase 5 OS=Arabidopsis thaliana OX=3702 GN=At3g52260 PE=2 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 2.4e-09
Identity = 49/132 (37.12%), Postives = 69/132 (52.27%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLLI 64
           P VAQGLYVA+  GKPAF KV VLE D     +LV+V           +   YM   L+ 
Sbjct: 232 PGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLV- 291

Query: 65  GGNSIWMTPPNPCPSFFNRHPLLETMHIL-SRSYQKAKKPVPGDYSYYLHAHQLVLYH-P 124
            G+ +++      P  F+   + +         Y++  + VPGD  Y+LHAHQ+ L +  
Sbjct: 292 -GDPLYVAGGQ--PKCFDPDLVDDAAAFAEDGGYRRPNQAVPGDCGYHLHAHQVELPNLL 351

Query: 125 RTNEVVKIVEPL 131
            T++VVKIV PL
Sbjct: 352 NTHKVVKIVAPL 359

BLAST of CmoCh04G027100 vs. ExPASy TrEMBL
Match: A0A6J1JY31 (RNA pseudouridine synthase 5 OS=Cucurbita maxima OX=3661 GN=LOC111489868 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.7e-21
Identity = 72/148 (48.65%), Postives = 88/148 (59.46%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLLI 64
           P VAQGLYVATSSGKPA+ KVNVLE DE+G+HTLVQV           +  + +   LL 
Sbjct: 231 PGVAQGLYVATSSGKPAYSKVNVLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLL- 290

Query: 65  GGNSIWMTPPNP-CPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVLYHPR 124
            G+ +++    P C        L++        YQ+  KPVPGD  YYLHAHQLVL+HP+
Sbjct: 291 -GDPLYVAGGQPNC----LHSELVDQSFAEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPK 350

Query: 125 TNEVVKIVEPL------RKGSEDARNGV 142
           T+EVVKIV PL         SEDA N V
Sbjct: 351 TSEVVKIVAPLPPILRTHGESEDAGNRV 372

BLAST of CmoCh04G027100 vs. ExPASy TrEMBL
Match: A0A1S3CPT2 (RNA pseudouridine synthase 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503310 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 3.8e-21
Identity = 70/147 (47.62%), Postives = 84/147 (57.14%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVTEDFFCLLSCNYMPKRLLIGGNS 64
           P VAQGLYVAT SGKPA  KVNVLE DE+GEHTLVQV          +  P ++ I  +S
Sbjct: 230 PGVAQGLYVATPSGKPACSKVNVLERDEQGEHTLVQVEIQ-------SGRPHQIRIHLSS 289

Query: 65  IWMTPPNPCPSFFNRHPLLETMHILSRS------YQKAKKPVPGDYSYYLHAHQLVLYHP 124
           I               P      ++++S      Y++  KPVPGD  YYLHAHQL+LYHP
Sbjct: 290 IGHPLLGDPLYVAGGQPNCLHSEVVNQSFAEDGGYERPMKPVPGDCGYYLHAHQLILYHP 349

Query: 125 RTNEVVKIVEPL------RKGSEDARN 140
           +TNEVVKIV PL      R  +ED  N
Sbjct: 350 KTNEVVKIVAPLPPILRTRSEAEDFHN 369

BLAST of CmoCh04G027100 vs. ExPASy TrEMBL
Match: A0A6J1FE06 (RNA pseudouridine synthase 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444674 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 6.5e-21
Identity = 72/150 (48.00%), Postives = 87/150 (58.00%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQV------TEDFFCLLSCNYMPKRL 64
           P VAQGLYVATSSGKPA+ KVNVLE DE+G+HTLVQV             LS    P   
Sbjct: 231 PGVAQGLYVATSSGKPAYSKVNVLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHP--- 290

Query: 65  LIGGNSIWMTPPNP-CPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVLYH 124
            + G+ +++    P C        L++        YQ+  KPVPGD  YYLHAHQLVL+H
Sbjct: 291 -LIGDPLYVAGGQPNC----LHSELVDQSFAEDGGYQRPTKPVPGDCGYYLHAHQLVLHH 350

Query: 125 PRTNEVVKIVEPL------RKGSEDARNGV 142
           P+T+EVVKI+ PL         SEDA N V
Sbjct: 351 PKTSEVVKILAPLPPILRTHGESEDAGNRV 372

BLAST of CmoCh04G027100 vs. ExPASy TrEMBL
Match: A0A6J1BWD7 (RNA pseudouridine synthase 5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006297 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.6e-19
Identity = 66/132 (50.00%), Postives = 78/132 (59.09%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLLI 64
           P VAQGLYVATSSGKPAF KV+VLE DE+G +TLVQV           +  + +   LL 
Sbjct: 231 PGVAQGLYVATSSGKPAFSKVDVLERDEQGNNTLVQVEIQSGRPHQIRIHLSSIGHPLL- 290

Query: 65  GGNSIWMT--PPNPCPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVLYHP 124
            G+ +++    PN   S        E        YQ+  KPVPGD  YYLHAHQLVL HP
Sbjct: 291 -GDPLYVAGGQPNCLHSDSVNQSFAE-----DGGYQRPTKPVPGDCGYYLHAHQLVLDHP 350

Query: 125 RTNEVVKIVEPL 131
           RTNE VKI+ PL
Sbjct: 351 RTNEAVKIIAPL 355

BLAST of CmoCh04G027100 vs. ExPASy TrEMBL
Match: A0A6J1BWA8 (RNA pseudouridine synthase 5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111006297 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.6e-19
Identity = 66/132 (50.00%), Postives = 78/132 (59.09%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLLI 64
           P VAQGLYVATSSGKPAF KV+VLE DE+G +TLVQV           +  + +   LL 
Sbjct: 218 PGVAQGLYVATSSGKPAFSKVDVLERDEQGNNTLVQVEIQSGRPHQIRIHLSSIGHPLL- 277

Query: 65  GGNSIWMT--PPNPCPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVLYHP 124
            G+ +++    PN   S        E        YQ+  KPVPGD  YYLHAHQLVL HP
Sbjct: 278 -GDPLYVAGGQPNCLHSDSVNQSFAE-----DGGYQRPTKPVPGDCGYYLHAHQLVLDHP 337

Query: 125 RTNEVVKIVEPL 131
           RTNE VKI+ PL
Sbjct: 338 RTNEAVKIIAPL 342

BLAST of CmoCh04G027100 vs. NCBI nr
Match: XP_038889122.1 (RNA pseudouridine synthase 5 isoform X1 [Benincasa hispida])

HSP 1 Score: 113.2 bits (282), Expect = 2.7e-21
Identity = 67/132 (50.76%), Postives = 79/132 (59.85%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVTEDFFCLLSCNYMPKRLLIGGNS 64
           P VAQGLYVAT SGKPA+ KVNVLE DE+G+HTLVQV          +  P ++ I  +S
Sbjct: 232 PGVAQGLYVATPSGKPAYSKVNVLERDEQGQHTLVQVEIQ-------SGRPHQIRIHLSS 291

Query: 65  IWMTPPNPCPSFFNRHPLLETMHILSRS------YQKAKKPVPGDYSYYLHAHQLVLYHP 124
           I               P      ++ +S      YQ+  KPVPGD  YYLHAHQLVLYHP
Sbjct: 292 IGHPLLGDPLYVAGGQPNCLHSEVVDQSFAEDGGYQRPMKPVPGDCGYYLHAHQLVLYHP 351

Query: 125 RTNEVVKIVEPL 131
           +TNEVVKIV PL
Sbjct: 352 KTNEVVKIVAPL 356

BLAST of CmoCh04G027100 vs. NCBI nr
Match: XP_022994066.1 (RNA pseudouridine synthase 5 [Cucurbita maxima])

HSP 1 Score: 112.8 bits (281), Expect = 3.5e-21
Identity = 72/148 (48.65%), Postives = 88/148 (59.46%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLLI 64
           P VAQGLYVATSSGKPA+ KVNVLE DE+G+HTLVQV           +  + +   LL 
Sbjct: 231 PGVAQGLYVATSSGKPAYSKVNVLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLL- 290

Query: 65  GGNSIWMTPPNP-CPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVLYHPR 124
            G+ +++    P C        L++        YQ+  KPVPGD  YYLHAHQLVL+HP+
Sbjct: 291 -GDPLYVAGGQPNC----LHSELVDQSFAEDGGYQRPTKPVPGDCGYYLHAHQLVLHHPK 350

Query: 125 TNEVVKIVEPL------RKGSEDARNGV 142
           T+EVVKIV PL         SEDA N V
Sbjct: 351 TSEVVKIVAPLPPILRTHGESEDAGNRV 372

BLAST of CmoCh04G027100 vs. NCBI nr
Match: KAG6578753.1 (RNA pseudouridine synthase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 112.5 bits (280), Expect = 4.6e-21
Identity = 72/150 (48.00%), Postives = 83/150 (55.33%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQV---------TEDFFCLLSCNYMP 64
           P VAQGLYVATSSGKPA+ KVNVLE DE+G+HTLVQV                +    M 
Sbjct: 231 PGVAQGLYVATSSGKPAYSKVNVLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMG 290

Query: 65  KRLLIGGNSIWMTPPNPCPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVL 124
             L + G       PN   S      L++        YQ+  KPVPGD  YYLHAHQLVL
Sbjct: 291 DPLYVAGGQ-----PNCLHS-----ELVDQSFAEDGGYQRPTKPVPGDCGYYLHAHQLVL 350

Query: 125 YHPRTNEVVKIVEPL------RKGSEDARN 140
           +HP+T+EVVKIV PL         SEDA N
Sbjct: 351 HHPKTSEVVKIVAPLPPILRTHGESEDAGN 370

BLAST of CmoCh04G027100 vs. NCBI nr
Match: KAG7016280.1 (RNA pseudouridine synthase 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 112.5 bits (280), Expect = 4.6e-21
Identity = 72/150 (48.00%), Postives = 83/150 (55.33%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQV---------TEDFFCLLSCNYMP 64
           P VAQGLYVATSSGKPA+ KVNVLE DE+G+HTLVQV                +    M 
Sbjct: 279 PGVAQGLYVATSSGKPAYSKVNVLERDEQGKHTLVQVEIQSGRPHQIRIHLSSIGHPLMG 338

Query: 65  KRLLIGGNSIWMTPPNPCPSFFNRHPLLETMHILSRSYQKAKKPVPGDYSYYLHAHQLVL 124
             L + G       PN   S      L++        YQ+  KPVPGD  YYLHAHQLVL
Sbjct: 339 DPLYVAGGQ-----PNCLHS-----ELVDQSFAEDGGYQRPTKPVPGDCGYYLHAHQLVL 398

Query: 125 YHPRTNEVVKIVEPL------RKGSEDARN 140
           +HP+T+EVVKIV PL         SEDA N
Sbjct: 399 HHPKTSEVVKIVAPLPPILRTHGESEDAGN 418

BLAST of CmoCh04G027100 vs. NCBI nr
Match: XP_008465672.1 (PREDICTED: RNA pseudouridine synthase 5 isoform X2 [Cucumis melo])

HSP 1 Score: 111.7 bits (278), Expect = 7.8e-21
Identity = 70/147 (47.62%), Postives = 84/147 (57.14%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVTEDFFCLLSCNYMPKRLLIGGNS 64
           P VAQGLYVAT SGKPA  KVNVLE DE+GEHTLVQV          +  P ++ I  +S
Sbjct: 230 PGVAQGLYVATPSGKPACSKVNVLERDEQGEHTLVQVEIQ-------SGRPHQIRIHLSS 289

Query: 65  IWMTPPNPCPSFFNRHPLLETMHILSRS------YQKAKKPVPGDYSYYLHAHQLVLYHP 124
           I               P      ++++S      Y++  KPVPGD  YYLHAHQL+LYHP
Sbjct: 290 IGHPLLGDPLYVAGGQPNCLHSEVVNQSFAEDGGYERPMKPVPGDCGYYLHAHQLILYHP 349

Query: 125 RTNEVVKIVEPL------RKGSEDARN 140
           +TNEVVKIV PL      R  +ED  N
Sbjct: 350 KTNEVVKIVAPLPPILRTRSEAEDFHN 369

BLAST of CmoCh04G027100 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 49/132 (37.12%), Postives = 69/132 (52.27%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLLI 64
           P VAQGLYVA+  GKPAF KV VLE D     +LV+V           +   YM   L+ 
Sbjct: 232 PGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLV- 291

Query: 65  GGNSIWMTPPNPCPSFFNRHPLLETMHIL-SRSYQKAKKPVPGDYSYYLHAHQLVLYH-P 124
            G+ +++      P  F+   + +         Y++  + VPGD  Y+LHAHQ+ L +  
Sbjct: 292 -GDPLYVAGGQ--PKCFDPDLVDDAAAFAEDGGYRRPNQAVPGDCGYHLHAHQVELPNLL 351

Query: 125 RTNEVVKIVEPL 131
            T++VVKIV PL
Sbjct: 352 NTHKVVKIVAPL 359

BLAST of CmoCh04G027100 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 58.5 bits (140), Expect = 7.3e-09
Identity = 49/147 (33.33%), Postives = 69/147 (46.94%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQVT----EDFFCLLSCNYMPKRLL- 64
           P VAQGLYVA+  GKPAF KV VLE D     +LV+V           +   YM   L+ 
Sbjct: 262 PGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPLVE 321

Query: 65  --------------IGGNSIWMTPPNPCPSFFNRHPLLETMHIL-SRSYQKAKKPVPGDY 124
                           G+ +++      P  F+   + +         Y++  + VPGD 
Sbjct: 322 MTMFTGSECLRADQSAGDPLYVAGGQ--PKCFDPDLVDDAAAFAEDGGYRRPNQAVPGDC 381

Query: 125 SYYLHAHQLVLYH-PRTNEVVKIVEPL 131
            Y+LHAHQ+ L +   T++VVKIV PL
Sbjct: 382 GYHLHAHQVELPNLLNTHKVVKIVAPL 406

BLAST of CmoCh04G027100 vs. TAIR 10
Match: AT3G52260.1 (Pseudouridine synthase family protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.1e-04
Identity = 23/37 (62.16%), Postives = 26/37 (70.27%), Query Frame = 0

Query: 5   PEVAQGLYVATSSGKPAFRKVNVLEIDERGEHTLVQV 42
           P VAQGLYVA+  GKPAF KV VLE D     +LV+V
Sbjct: 232 PGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKV 268

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0DST93.3e-1439.42RNA pseudouridine synthase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g02... [more]
Q5M7212.4e-0937.12RNA pseudouridine synthase 5 OS=Arabidopsis thaliana OX=3702 GN=At3g52260 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JY311.7e-2148.65RNA pseudouridine synthase 5 OS=Cucurbita maxima OX=3661 GN=LOC111489868 PE=4 SV... [more]
A0A1S3CPT23.8e-2147.62RNA pseudouridine synthase 5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503310 ... [more]
A0A6J1FE066.5e-2148.00RNA pseudouridine synthase 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1BWD74.6e-1950.00RNA pseudouridine synthase 5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1BWA84.6e-1950.00RNA pseudouridine synthase 5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038889122.12.7e-2150.76RNA pseudouridine synthase 5 isoform X1 [Benincasa hispida][more]
XP_022994066.13.5e-2148.65RNA pseudouridine synthase 5 [Cucurbita maxima][more]
KAG6578753.14.6e-2148.00RNA pseudouridine synthase 5, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7016280.14.6e-2148.00RNA pseudouridine synthase 5 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_008465672.17.8e-2147.62PREDICTED: RNA pseudouridine synthase 5 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G52260.21.7e-1037.12Pseudouridine synthase family protein [more]
AT3G52260.37.3e-0933.33Pseudouridine synthase family protein [more]
AT3G52260.11.1e-0462.16Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR21600:SF44RNA PSEUDOURIDINE SYNTHASE 5coord: 92..132
coord: 4..41
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 92..132
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 4..41

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G027100.1CmoCh04G027100.1mRNA