CmoCh04G023650 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh04G023650 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.0e-232
Identity = 383/547 (70.02%), Postives = 458/547 (83.73%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPA+SSPLTPLGFLERAAT+YGD  S++Y   +YTWS THRRCL LASS++S+G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           I+ GHVVSV++PN P MYELHFAVPM GAILN +N RLDARTISILL HSESKLIFVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
             +LI  ++ LFP +   P LV +AD++   ES +S  +   F  SY+++I +GD +F+W
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADES---ESGNSSELGKEFFCSYKDLIDRGDPDFKW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           + P SEWDP++LNYTSGTTSSPKGVVHCHRG FI+T+D L++WGVPKQ VYLWTLPMFHA
Sbjct: 181 VMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWS+ WG+AAVGGTNIC+RKFDS ++Y +I+RH VTH+CGAPVVLNML+N+P   PL  
Sbjct: 241 NGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKT 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
            V+I+TAGAP P+ +L R ESLGF V+HGYGLTET G VV C+WK EWN LPA ERARLK
Sbjct: 301 TVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGV TV    ++VVD  TG +V RDG ++GE+VLR GS+MLGY KD E T KSMT +G
Sbjct: 361 SRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WF+TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILY++P + EAAVVARPDEF
Sbjct: 421 WFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSL++GLT+KPTE+EI+E+C+ KLPR+MVPKTVVF  ELPKTSTGK+QK++L
Sbjct: 481 WGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFIL 540

Query: 541 REMAMSM 548
           R+MA  M
Sbjct: 541 RDMARGM 544

BLAST of CmoCh04G023650 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 2.8e-214
Identity = 357/556 (64.21%), Postives = 447/556 (80.40%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSS-I 60
           MEELKPR ANS PLTPLGFLERAAT+YGD  S++Y   +YTWS TH RCL++AS + S +
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           G+++G VVSV++PN P MYEL+FAVPM G +LN INTRL+A TIS++L HSESKL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 121 AFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSE-F 180
               L+ +++ LFP     P+LVLIAD+   E  + +V    +FV  YE+++ KGD + F
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGE--NLTVEDREHFVCCYEDLVEKGDDKTF 180

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           +W+RP SEWDPIVLNYTSGTTSSPKGVVH HR  F+VTLD L++WGVPKQ VYLWTLPMF
Sbjct: 181 KWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMF 240

Query: 241 HANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPL 300
           HANGW ++WGIAAVGGTNIC+R+FD  ++++LIRRH VTH+C AP+VLNML+NSPN  PL
Sbjct: 241 HANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPL 300

Query: 301 DWPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERAR 360
             PV ++T GAP PA +LLR ESLGF ++HGYG+TE  G VV C+WK EWN+LPA E+AR
Sbjct: 301 PNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQAR 360

Query: 361 LKSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTE 420
           LKSRQGVRT A   V+VVD  +G SV RDG+++GEIVL+  S+MLGY K+  AT K +  
Sbjct: 361 LKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRA 420

Query: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPD 480
           +GWF+TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++P+V+EAAVVA PD
Sbjct: 421 DGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPD 480

Query: 481 EFWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKY 540
           E+WGETP AFV+L++G+  +PTE+EI+E+C+ KL  FMVPK VVF  +LPKTSTGKIQK+
Sbjct: 481 EYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKF 540

Query: 541 LLREMAMSMDPTKRSS 555
            L+E+  +M  +  SS
Sbjct: 541 TLKEIVKTMGYSSSSS 554

BLAST of CmoCh04G023650 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 9.1e-213
Identity = 356/554 (64.26%), Postives = 447/554 (80.69%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYG-HTTYTWSHTHRRCLQLASSLSSI 60
           ME++KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQ 180
              +L   ++ +   +   P+LV IAD    E   + V     F  +Y+++I +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIADKE-EEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLD 300
           ANGWS+ WGIAAVGGTN+C+RKFD+ L+Y LIR H VTH+CGAPVVLNML+ +   +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 WPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARL 360
            PV ILTAGAP PA +LLR ES+GF ++HGYGLTET G  V C+WK +WN+LPA +RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEE 420
           K+RQGVRTV    ++VVD E+G+SV R+G ++GEIV+R  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYTNP+VNE AVVARPD 
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ GLTQ+PTE E+IE+C++K+P++MVPKTV F+ ELPKTSTGK+ K++
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LREMAMSMDPTKRS 554
           LRE+A  M  T+ S
Sbjct: 541 LREIAKKMGTTRLS 550

BLAST of CmoCh04G023650 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 4.2e-210
Identity = 351/548 (64.05%), Postives = 443/548 (80.84%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYG-HTTYTWSHTHRRCLQLASSLSSI 60
           MEE+KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQ 180
              +L   ++ +   +   P+LV+IAD    E   + V     F  +Y+++I +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIADKE-EEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLD 300
           +NGW+  WGIAAVGGTN+C+RKFD+ L+Y LIR H VTH+CGAPVVLNML+ +   +PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 WPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARL 360
            PV ILTAG+P PAT+LLR ES+GF ++HGYGLTET G +V C+WK +WN LPA +RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEE 420
           K+RQGVRTV    ++VVD E+G SV R+G ++GEIV+R  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYT P+VNE AVVARPDE
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ G + KPTEEE++E+C++K+P++MVPKTV FM ELPK+STGK+ K++
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LREMAMSM 548
           LR++A  M
Sbjct: 541 LRDIAKKM 544

BLAST of CmoCh04G023650 vs. ExPASy Swiss-Prot
Match: Q9LQS1 (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana OX=3702 GN=AAE8 PE=2 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 4.8e-206
Identity = 356/549 (64.85%), Postives = 438/549 (79.78%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTT-YTWSHTHRRCLQLASSLSSI 60
           ME+LKP  ANS PLT LGFLERAAT+YGD  SI+YG++T YTW  T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQ 180
            + +L   ++ +    +N P+LVLIA++   EE  + V     F   Y ++I +G+ +F+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEE-EEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG F++TLD L +W VPK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNS-PNIRPL 300
           ANGW++ WGIAAVGGTN+C+RK  +  +Y LIR H VTH+ GAP+VL +L+ S  + +PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DWPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERAR 360
             PV  LTAG+  PAT+LLR ESLGF V+HGYGLTET G +V C+WK  WN+LPA ++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTE 420
           LKSRQGVRTV    ++VVD E+G+SV RDG ++GEIVLR  S+MLGY K+   T  S  +
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPD 480
            GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LYTNP+VNEAAVVARPD
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAFVSL+ GLT+KPT++EIIE+CK K+PR+M PKTV F+ ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 LLREMAMSM 548
           LL+E+A +M
Sbjct: 541 LLKEIAKNM 544

BLAST of CmoCh04G023650 vs. ExPASy TrEMBL
Match: A0A6J1E5Q3 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430192 PE=4 SV=1)

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG
Sbjct: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW
Sbjct: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW
Sbjct: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG
Sbjct: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDPTKRSSKKN 558
           REMAMSMDPTKRSSKKN
Sbjct: 541 REMAMSMDPTKRSSKKN 557

BLAST of CmoCh04G023650 vs. ExPASy TrEMBL
Match: A0A6J1E3B0 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430201 PE=4 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.7e-307
Identity = 515/549 (93.81%), Postives = 539/549 (98.18%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPANSSPLTP+GFLERAATIYGDS SIIY HTTYTWSHTHRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHN+LDLFPPE+NRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDS+F+W
Sbjct: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ LVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGF+VTHGYGLTETGGTVVCCSWKAEW+QLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDG+S+GE+VL+VGSLMLGYFKDLEAT KSMTEEG
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNP+VNEAAVVARP EF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT++PTEEEIIE+CK KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDP 550
           +EMAMSM P
Sbjct: 541 KEMAMSMGP 549

BLAST of CmoCh04G023650 vs. ExPASy TrEMBL
Match: A0A6J1JT47 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487610 PE=4 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 6.6e-307
Identity = 522/557 (93.72%), Postives = 538/557 (96.59%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPANSSPLTP+GFLERAATIY DS SIIYG+TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCH ESKLIFVD A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHGESKLIFVDHA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHN+LDLFPPEI RPMLVLIADDA AEESSSSVLIEGNFV SYE MIRKGDSEFQW
Sbjct: 121 FCNLIHNALDLFPPEIYRPMLVLIADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ LVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGFDVTHGYGLTETGGTVVCCSWKAEW+QLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDGVS+GEIVLRVGSLMLGYFKDLEAT KSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NPSVNEAAVVARP EF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTQ+ TEEEIIE+C+ KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQRTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDPTKRSSKKN 558
           REMAMSM PTKRSSKKN
Sbjct: 541 REMAMSMGPTKRSSKKN 557

BLAST of CmoCh04G023650 vs. ExPASy TrEMBL
Match: A0A6J1JUG4 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487609 PE=4 SV=1)

HSP 1 Score: 1054.7 bits (2726), Expect = 1.4e-304
Identity = 515/549 (93.81%), Postives = 533/549 (97.09%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME LKPRPANSSPLTP+GFLERAATIY DS SIIYG+TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVD+A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHN+LDLFPPEINRPMLVL+ADDA AEESSSSVLIEGNFV SYE MIRKGDSEFQW
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ LVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGFDVTHGYGLTETGGTVVCCSWKAEW+QLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDGVS+GEIVLRVGSLMLGYFKDLEAT KSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NPSVNEAAVVARP EF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTQ+PTEEEIIE+C+ KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDP 550
           +EMAMSM P
Sbjct: 541 KEMAMSMGP 549

BLAST of CmoCh04G023650 vs. ExPASy TrEMBL
Match: A0A6J1JKT9 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111487797 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 9.3e-277
Identity = 474/552 (85.87%), Postives = 502/552 (90.94%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPANSSPLTPLGFLERAATIYGDS SIIYG TTYTWS TH RCLQLASSLSS+G
Sbjct: 70  MEDLKPRPANSSPLTPLGFLERAATIYGDSPSIIYGDTTYTWSDTHCRCLQLASSLSSMG 129

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGH+VSVISPNTPPMYELHFAVPM GAILNTINTRLDARTISILL HSESKLIFVDQA
Sbjct: 130 IQRGHIVSVISPNTPPMYELHFAVPMAGAILNTINTRLDARTISILLTHSESKLIFVDQA 189

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSS-VLIEGNFVGSYEEMIRKGDSEFQ 180
           FC LIH++L LFPPE  RPMLVLI DDA AEESSSS ++I  +FV SYEEMI KGDS+F+
Sbjct: 190 FCALIHDALALFPPETKRPMLVLITDDAAAEESSSSCIMIHDDFVDSYEEMILKGDSDFR 249

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPIVLNYTSGTTSSPKGVV CHRG FI+TLD+LLEW V KQSVYLWTLPMFH
Sbjct: 250 WIRPVSEWDPIVLNYTSGTTSSPKGVVQCHRGIFIITLDVLLEWEVQKQSVYLWTLPMFH 309

Query: 241 ANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLD 300
            NGWS +WG+AAVGGTNICIRKFD+AL+Y+LIRRHHVTHLCGAPVVLNMLTNSP  RP D
Sbjct: 310 GNGWSLTWGMAAVGGTNICIRKFDAALIYTLIRRHHVTHLCGAPVVLNMLTNSPTNRPFD 369

Query: 301 WPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARL 360
           WPVKILT GAP P+ +LLR ESLGFDVTHGYGLTET G VV CSWKAEWNQLP  ERARL
Sbjct: 370 WPVKILTCGAPPPSEVLLRTESLGFDVTHGYGLTETAGPVVFCSWKAEWNQLPTKERARL 429

Query: 361 KSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEE 420
           KSRQGVRTVA  AV+VVD +TGKSV +DGVSIGEIVL+ GS+MLGY KD +AT KSMTEE
Sbjct: 430 KSRQGVRTVAMTAVDVVDQKTGKSVKQDGVSIGEIVLKGGSIMLGYLKDRDATTKSMTEE 489

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDE 480
           GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NPSVNEAAVVARPDE
Sbjct: 490 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPSVNEAAVVARPDE 549

Query: 481 FWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVS+REGLTQKPTEEEIIE C+ KLPRFMVPKTVVFMTELPKTSTGKIQKYL
Sbjct: 550 FWGETPCAFVSIREGLTQKPTEEEIIEHCRGKLPRFMVPKTVVFMTELPKTSTGKIQKYL 609

Query: 541 LREMAMSMDPTK 552
           LREMAMSM   K
Sbjct: 610 LREMAMSMGRAK 621

BLAST of CmoCh04G023650 vs. NCBI nr
Match: XP_022922128.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 557/557 (100.00%), Postives = 557/557 (100.00%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG
Sbjct: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW
Sbjct: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW
Sbjct: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG
Sbjct: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDPTKRSSKKN 558
           REMAMSMDPTKRSSKKN
Sbjct: 541 REMAMSMDPTKRSSKKN 557

BLAST of CmoCh04G023650 vs. NCBI nr
Match: KAG6601999.1 (putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1064.7 bits (2752), Expect = 2.7e-307
Identity = 519/549 (94.54%), Postives = 538/549 (98.00%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPANSSPLTP+GFLERAA IYGDS SIIYGHTTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAAIIYGDSPSIIYGHTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHN+LDLFPPEINRPMLVLIADDAVAEESSSSVLI+G+F+GSYEEMIRKGDS+F+W
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLIADDAVAEESSSSVLIDGDFLGSYEEMIRKGDSDFRW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ALVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAALVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPA ERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAEERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDGVS+GEIVLRVGSLMLGYFKDLEAT KSMTEEG
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARP EF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTQ+PTEEEIIE+CK KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDP 550
           +EMAMSM P
Sbjct: 541 KEMAMSMGP 549

BLAST of CmoCh04G023650 vs. NCBI nr
Match: XP_022922136.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.6e-307
Identity = 515/549 (93.81%), Postives = 539/549 (98.18%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPANSSPLTP+GFLERAATIYGDS SIIY HTTYTWSHTHRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYGDSPSIIYAHTTYTWSHTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHN+LDLFPPE+NRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDS+F+W
Sbjct: 121 FCNLIHNALDLFPPEVNRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSDFRW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPI LNYTSGTTSSPKGVVHCHRGSFI+T+DMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPISLNYTSGTTSSPKGVVHCHRGSFIITMDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ LVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDATLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGF+VTHGYGLTETGGTVVCCSWKAEW+QLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFEVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDG+S+GE+VL+VGSLMLGYFKDLEAT KSMTEEG
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGLSMGEVVLKVGSLMLGYFKDLEATAKSMTEEG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNP+VNEAAVVARP EF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPTVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT++PTEEEIIE+CK KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTEQPTEEEIIEYCKGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDP 550
           +EMAMSM P
Sbjct: 541 KEMAMSMGP 549

BLAST of CmoCh04G023650 vs. NCBI nr
Match: XP_022990839.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.4e-306
Identity = 522/557 (93.72%), Postives = 538/557 (96.59%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME+LKPRPANSSPLTP+GFLERAATIY DS SIIYG+TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEDLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCH ESKLIFVD A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHGESKLIFVDHA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHN+LDLFPPEI RPMLVLIADDA AEESSSSVLIEGNFV SYE MIRKGDSEFQW
Sbjct: 121 FCNLIHNALDLFPPEIYRPMLVLIADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ LVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGFDVTHGYGLTETGGTVVCCSWKAEW+QLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDGVS+GEIVLRVGSLMLGYFKDLEAT KSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NPSVNEAAVVARP EF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTQ+ TEEEIIE+C+ KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQRTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDPTKRSSKKN 558
           REMAMSM PTKRSSKKN
Sbjct: 541 REMAMSMGPTKRSSKKN 557

BLAST of CmoCh04G023650 vs. NCBI nr
Match: XP_022990838.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1054.7 bits (2726), Expect = 2.8e-304
Identity = 515/549 (93.81%), Postives = 533/549 (97.09%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTTYTWSHTHRRCLQLASSLSSIG 60
           ME LKPRPANSSPLTP+GFLERAATIY DS SIIYG+TTYTWS THRRCLQLASSLSSIG
Sbjct: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQA 120
           IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVD+A
Sbjct: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120

Query: 121 FCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQW 180
           FCNLIHN+LDLFPPEINRPMLVL+ADDA AEESSSSVLIEGNFV SYE MIRKGDSEFQW
Sbjct: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA
Sbjct: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240

Query: 241 NGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLDW 300
           NGWSFSWG+AAVGGTNICIRKFD+ LVYSLIRRHHVTHLCGAPVVLNMLTNSP+IRPLDW
Sbjct: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300

Query: 301 PVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARLK 360
           PVKILTAGAPVPATIL RIESLGFDVTHGYGLTETGGTVVCCSWKAEW+QLPAVERARLK
Sbjct: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360

Query: 361 SRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEEG 420
           SRQGVRTVATPAV+VVDLETG+SVARDGVS+GEIVLRVGSLMLGYFKDLEAT KSMTE+G
Sbjct: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420

Query: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY NPSVNEAAVVARP EF
Sbjct: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480

Query: 481 WGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLTQ+PTEEEIIE+C+ KLPRFM PKTVVFMTELPKTSTGKIQKYLL
Sbjct: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540

Query: 541 REMAMSMDP 550
           +EMAMSM P
Sbjct: 541 KEMAMSMGP 549

BLAST of CmoCh04G023650 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 741.1 bits (1912), Expect = 6.5e-214
Identity = 356/554 (64.26%), Postives = 447/554 (80.69%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYG-HTTYTWSHTHRRCLQLASSLSSI 60
           ME++KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQ 180
              +L   ++ +   +   P+LV IAD    E   + V     F  +Y+++I +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIADKE-EEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLD 300
           ANGWS+ WGIAAVGGTN+C+RKFD+ L+Y LIR H VTH+CGAPVVLNML+ +   +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 WPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARL 360
            PV ILTAGAP PA +LLR ES+GF ++HGYGLTET G  V C+WK +WN+LPA +RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEE 420
           K+RQGVRTV    ++VVD E+G+SV R+G ++GEIV+R  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYTNP+VNE AVVARPD 
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ GLTQ+PTE E+IE+C++K+P++MVPKTV F+ ELPKTSTGK+ K++
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LREMAMSMDPTKRS 554
           LRE+A  M  T+ S
Sbjct: 541 LREIAKKMGTTRLS 550

BLAST of CmoCh04G023650 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 732.3 bits (1889), Expect = 3.0e-211
Identity = 351/548 (64.05%), Postives = 443/548 (80.84%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYG-HTTYTWSHTHRRCLQLASSLSSI 60
           MEE+KP  ANS PLTP+GFLERAAT+YGD  SI+YG +T YTW  T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQ 180
              +L   ++ +   +   P+LV+IAD    E   + V     F  +Y+++I +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIADKE-EEGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRPLD 300
           +NGW+  WGIAAVGGTN+C+RKFD+ L+Y LIR H VTH+CGAPVVLNML+ +   +PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 WPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERARL 360
            PV ILTAG+P PAT+LLR ES+GF ++HGYGLTET G +V C+WK +WN LPA +RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTEE 420
           K+RQGVRTV    ++VVD E+G SV R+G ++GEIV+R  S+MLGY KD   T K++ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPDE 480
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE++LYT P+VNE AVVARPDE
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ G + KPTEEE++E+C++K+P++MVPKTV FM ELPK+STGK+ K++
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LREMAMSM 548
           LR++A  M
Sbjct: 541 LRDIAKKM 544

BLAST of CmoCh04G023650 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 718.8 bits (1854), Expect = 3.4e-207
Identity = 356/549 (64.85%), Postives = 438/549 (79.78%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIYGHTT-YTWSHTHRRCLQLASSLSSI 60
           ME+LKP  ANS PLT LGFLERAAT+YGD  SI+YG++T YTW  T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDQ 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSEFQ 180
            + +L   ++ +    +N P+LVLIA++   EE  + V     F   Y ++I +G+ +F+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEE-EEEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG F++TLD L +W VPK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNS-PNIRPL 300
           ANGW++ WGIAAVGGTN+C+RK  +  +Y LIR H VTH+ GAP+VL +L+ S  + +PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DWPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERAR 360
             PV  LTAG+  PAT+LLR ESLGF V+HGYGLTET G +V C+WK  WN+LPA ++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMTE 420
           LKSRQGVRTV    ++VVD E+G+SV RDG ++GEIVLR  S+MLGY K+   T  S  +
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARPD 480
            GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE++LYTNP+VNEAAVVARPD
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAFVSL+ GLT+KPT++EIIE+CK K+PR+M PKTV F+ ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 LLREMAMSM 548
           LL+E+A +M
Sbjct: 541 LLKEIAKNM 544

BLAST of CmoCh04G023650 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 692.2 bits (1785), Expect = 3.4e-199
Identity = 339/555 (61.08%), Postives = 428/555 (77.12%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIY-GHTTYTWSHTHRRCLQLASSL--S 60
           ME L P P+NS+PLT LGFL+RAA++YGD  SI++  +T +TWS TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFV 120
           S+GI RG VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DQAFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSE 180
           D    +++  ++  F  +  +P LVL+ DD   E+ S S     +F+ +Y+ ++ +GDS 
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDD--QEDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 181 FQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPM 240
           F+WIRP +EW P++LNYTSGTTSSPKGVV  HR  F++T+  LL+W  P + VYLWTLPM
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 241 FHANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRP 300
           FHANGW ++WG AAVG TN+C R+ D+  +Y LI +HHVTH+C AP+VLNMLTN P+ +P
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 301 LDWPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERA 360
           L  PV+++TAGAP PA I+ R E+LGF+V HGYGLTETGG VV C+WKAEW+ L  +ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 361 RLKSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMT 420
           RLKSRQGVRT+    V+V D  TGKSV  DGVS+GEIVL+ GS+MLGY+KD E T   M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 421 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARP 480
           E+GWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE++LYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 481 DEFWGETPCAFVSLR-----EGLTQKPTEEEIIEFCKRKLPRFMVPKTVVFMTELPKTST 540
           D+ WGETPCAFVSL+      GL    TE EI EFCK +LP++MVP+ V+F  ELPKTST
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKIQKYLLREMAMSM 548
           GKIQK+LLR+MA S+
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CmoCh04G023650 vs. TAIR 10
Match: AT1G21530.2 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 685.3 bits (1767), Expect = 4.2e-197
Identity = 336/554 (60.65%), Postives = 429/554 (77.44%), Query Frame = 0

Query: 1   MEELKPRPANSSPLTPLGFLERAATIYGDSASIIY-GHTTYTWSHTHRRCLQLASSL--S 60
           ME L P P+NS+PLT LGFL+RAA++YGD  SI++  +T +TWS TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTANTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFV 120
           SIGI++G VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSES+L+FV
Sbjct: 61  SIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESRLVFV 120

Query: 121 DQAFCNLIHNSLDLFPPEINRPMLVLIADDAVAEESSSSVLIEGNFVGSYEEMIRKGDSE 180
           D    +L+  ++ LF  +  +P LVL+ DD   + SS+S     +F+ +YEE++ +G+S 
Sbjct: 121 DHRSISLVLEAVSLF-TQHEKPHLVLLDDDQENDSSSAS-----DFLDTYEEIMERGNSR 180

Query: 181 FQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPM 240
           F+WIRP +EW P+VLNYTSGTTSSPKGVV  HR  F++T+  LL+W VP + VYLWTLPM
Sbjct: 181 FKWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWSVPNRPVYLWTLPM 240

Query: 241 FHANGWSFSWGIAAVGGTNICIRKFDSALVYSLIRRHHVTHLCGAPVVLNMLTNSPNIRP 300
           FHANGW ++WG AAVG TNIC R+ D+  +Y+LI +H+VTH+C AP+VLNML N P   P
Sbjct: 241 FHANGWGYTWGTAAVGATNICTRRVDAPTIYNLIDKHNVTHMCAAPMVLNMLINYPLSTP 300

Query: 301 LDWPVKILTAGAPVPATILLRIESLGFDVTHGYGLTETGGTVVCCSWKAEWNQLPAVERA 360
           L  PV+++T+GAP PATI+ R ESLGF+V+H YGLTET G VV C+WK +W+ L  +ERA
Sbjct: 301 LKNPVQVMTSGAPPPATIISRAESLGFNVSHSYGLTETSGPVVSCAWKPKWDHLDPLERA 360

Query: 361 RLKSRQGVRTVATPAVNVVDLETGKSVARDGVSIGEIVLRVGSLMLGYFKDLEATVKSMT 420
           RLKSRQGVRT+    V+V D +TGKSV  DGVS+GEIV R  S+MLGY+KD + T   M 
Sbjct: 361 RLKSRQGVRTLGFTEVDVRDRKTGKSVKHDGVSVGEIVFRGSSVMLGYYKDPQGTAACMR 420

Query: 421 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYTNPSVNEAAVVARP 480
           E+GWF++GD+GV+H DGYLEIKDRSKDVII GGEN+SS E+E++LYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKP 480

Query: 481 DEFWGETPCAFVSLR---EGLTQKP-TEEEIIEFCKRKLPRFMVPKTVVFMTELPKTSTG 540
           D+ WGETPCAFVSL+    G    P TE EI EFCK KLP++MVP+ V+F  ELPKTSTG
Sbjct: 481 DKMWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTG 540

Query: 541 KIQKYLLREMAMSM 548
           KIQK+LLR+MA ++
Sbjct: 541 KIQKFLLRQMAKTL 548