CmoCh04G020530 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G020530
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionsuccinate dehydrogenase assembly factor 2, mitochondrial
LocationCmo_Chr04: 11461190 .. 11462445 (+)
RNA-Seq ExpressionCmoCh04G020530
SyntenyCmoCh04G020530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAAAGAAGAAAGGTATAGCCGATGAGAAGGTGAGGTGGGTAACAGATGAAGAAACGATGAAGTAAGGACTTGTTAGTGGTAACCGGTGGTGGAGGAAGCAGAAGGACAAAAGATTTGCAAATAATTAAAAAAAATGAACAACCTAACGACTCGACCCAACAAAAAATAGAGGGTTGGATTAGATTGTGACGTCTATTTAGATCATTCGGATCATCATTTTTGGATTGGTCCAATAATATTTCTCAACCCAACCTTACCGCGCAGACTCCTACTCATTTCTATCCCCATGGCCAGCTATTATAGGGCCTTCATGGCACTAGTCCACTAACTATTAAAATCAAGGGTAACCGCCAATGCCTAGTGACAAAGGGAATGGTGGAGGAGATAGTTTCATTAAGTGTGAAAGTTACAACAATGGGTAGTAAAATCGAGGGGAAGGGTTTGAGTTTAGTGTTCCTTACCACCAAAATAGGTGATTTCAGTTCAGTCCATCTGCGGAAACTGGGTATTGCTGATTTCAGTTCCTCATTTTTCTTTCTTTCTTTCTTTTAAATCTATTGTAAATGATCTTGAGGACTACTTTTTTGGATGCTGCTTATTTCTTTTGCAAGTTTCTGTATGTGTTCTTCTGTTGTTCAGGAAAATCCAGATCTATGGAAGTGGTTAACCGGGCAAGAGCAACCCCCTGAAGCCGTGAAGACAAATCCTGTGAGCATCCAAATTAGTGTTTCCCTTTTTATGTTTGGACTAAAAAATATGTGATCTTAGAATTAGAGCATCTATTTGTAGGTATTTACTGATGTGCAAGAGAAGGTGATGAACAATCTCAACAAACATGCCTCACCCGAGACACGAACACCGCCTGGGCAACAATGGGTAAGGGGTTGGGATGATTTCAAGAGAGGTCGGGATGGCCCCATAACAGGAAACCAGTAGCTCTATCTTCCATTAAAGAATTTTATGTTATAATTAAGTACTAAAGTTTCTCAAACTTGTTTGTTACGAACGGTGGGCTGTGAATAAGTTTCCCATCAAGTCTGTCCCCTCTAAACCCTTGGGATCTATAGTGAAATAATGGCTGTTTTTGTTTCTTTCACATACATTCACCAAATAATTCATCATTTCCAAGTGCTTGCCACCATGGCTAGCTTGCATTTCTTTATAGTTTGTACTTGATGTAATAAGCTTTGTATTAATTTTATAGTTTGCATTAGTATTACGACCTTCAATATCGGGTCATAAACCATCCG

mRNA sequence

ATGGAGGAAAGAAGAAAGGAAAATCCAGATCTATGGAAGTGGTTAACCGGGCAAGAGCAACCCCCTGAAGCCGTGAAGACAAATCCTGTATTTACTGATGTGCAAGAGAAGGTGATGAACAATCTCAACAAACATGCCTCACCCGAGACACGAACACCGCCTGGGCAACAATGGGTAAGGGGTTGGGATGATTTCAAGAGAGGTCGGGATGGCCCCATAACAGGAAACCAGTAGCTCTATCTTCCATTAAAGAATTTTATGTTATAATTAAGTACTAAAGTTTCTCAAACTTGTTTGTTACGAACGGTGGGCTGTGAATAAGTTTCCCATCAAGTCTGTCCCCTCTAAACCCTTGGGATCTATAGTGAAATAATGGCTGTTTTTGTTTCTTTCACATACATTCACCAAATAATTCATCATTTCCAAGTGCTTGCCACCATGGCTAGCTTGCATTTCTTTATAGTTTGTACTTGATGTAATAAGCTTTGTATTAATTTTATAGTTTGCATTAGTATTACGACCTTCAATATCGGGTCATAAACCATCCG

Coding sequence (CDS)

ATGGAGGAAAGAAGAAAGGAAAATCCAGATCTATGGAAGTGGTTAACCGGGCAAGAGCAACCCCCTGAAGCCGTGAAGACAAATCCTGTATTTACTGATGTGCAAGAGAAGGTGATGAACAATCTCAACAAACATGCCTCACCCGAGACACGAACACCGCCTGGGCAACAATGGGTAAGGGGTTGGGATGATTTCAAGAGAGGTCGGGATGGCCCCATAACAGGAAACCAGTAG

Protein sequence

MEERRKENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFKRGRDGPITGNQ
Homology
BLAST of CmoCh04G020530 vs. ExPASy Swiss-Prot
Match: Q9FI44 (Succinate dehydrogenase assembly factor 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SDHAF2 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 8.6e-29
Identity = 56/71 (78.87%), Postives = 61/71 (85.92%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLT QEQPPEAV +NPVF+ + EKVM NLNKHA+PETR   GQ WVRGWDDFK
Sbjct: 118 ENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHAAPETRAAAGQPWVRGWDDFK 177

Query: 67  RGRDGPITGNQ 78
           RGRD PI+GNQ
Sbjct: 178 RGRDAPISGNQ 188

BLAST of CmoCh04G020530 vs. ExPASy TrEMBL
Match: A0A6J1EQG7 (succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111436468 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 7.5e-36
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK
Sbjct: 131 ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 190

Query: 67  RGRDGPITGNQ 78
           RGRDGPITGNQ
Sbjct: 191 RGRDGPITGNQ 201

BLAST of CmoCh04G020530 vs. ExPASy TrEMBL
Match: A0A6J1JZA0 (succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111490192 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.2e-35
Identity = 70/71 (98.59%), Postives = 71/71 (100.00%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLTGQEQPP+AVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK
Sbjct: 131 ENPDLWKWLTGQEQPPKAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 190

Query: 67  RGRDGPITGNQ 78
           RGRDGPITGNQ
Sbjct: 191 RGRDGPITGNQ 201

BLAST of CmoCh04G020530 vs. ExPASy TrEMBL
Match: A0A5D3C9Y9 (Succinate dehydrogenase assembly factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G001200 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 7.1e-34
Identity = 67/71 (94.37%), Postives = 70/71 (98.59%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLTGQEQPPEA+KTNPVFT V+EKVMNNLNKHASPETRTPPGQQWVRGWDDFK
Sbjct: 130 ENPDLWKWLTGQEQPPEALKTNPVFTGVKEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 189

Query: 67  RGRDGPITGNQ 78
           +GRDGPITGNQ
Sbjct: 190 KGRDGPITGNQ 200

BLAST of CmoCh04G020530 vs. ExPASy TrEMBL
Match: A0A1S3B413 (succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103485912 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 7.1e-34
Identity = 67/71 (94.37%), Postives = 70/71 (98.59%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLTGQEQPPEA+KTNPVFT V+EKVMNNLNKHASPETRTPPGQQWVRGWDDFK
Sbjct: 130 ENPDLWKWLTGQEQPPEALKTNPVFTGVKEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 189

Query: 67  RGRDGPITGNQ 78
           +GRDGPITGNQ
Sbjct: 190 KGRDGPITGNQ 200

BLAST of CmoCh04G020530 vs. ExPASy TrEMBL
Match: A0A0A0LKF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G072460 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 7.8e-33
Identity = 65/71 (91.55%), Postives = 70/71 (98.59%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLTGQEQPPEA+KTNPVFT V+EKV++NLNKHASPETRTPPGQQWVRGWDDFK
Sbjct: 195 ENPDLWKWLTGQEQPPEALKTNPVFTGVKEKVIDNLNKHASPETRTPPGQQWVRGWDDFK 254

Query: 67  RGRDGPITGNQ 78
           +GRDGPITGNQ
Sbjct: 255 KGRDGPITGNQ 265

BLAST of CmoCh04G020530 vs. TAIR 10
Match: AT5G51040.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 (InterPro:IPR005631); Has 532 Blast hits to 532 proteins in 207 species: Archae - 0; Bacteria - 285; Metazoa - 16; Fungi - 41; Plants - 40; Viruses - 0; Other Eukaryotes - 150 (source: NCBI BLink). )

HSP 1 Score: 127.1 bits (318), Expect = 6.1e-30
Identity = 56/71 (78.87%), Postives = 61/71 (85.92%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLT QEQPPEAV +NPVF+ + EKVM NLNKHA+PETR   GQ WVRGWDDFK
Sbjct: 118 ENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHAAPETRAAAGQPWVRGWDDFK 177

Query: 67  RGRDGPITGNQ 78
           RGRD PI+GNQ
Sbjct: 178 RGRDAPISGNQ 188

BLAST of CmoCh04G020530 vs. TAIR 10
Match: AT5G51040.2 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 (InterPro:IPR005631); Has 368 Blast hits to 368 proteins in 141 species: Archae - 0; Bacteria - 216; Metazoa - 8; Fungi - 13; Plants - 40; Viruses - 0; Other Eukaryotes - 91 (source: NCBI BLink). )

HSP 1 Score: 127.1 bits (318), Expect = 6.1e-30
Identity = 56/71 (78.87%), Postives = 61/71 (85.92%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLT QEQPPEAV +NPVF+ + EKVM NLNKHA+PETR   GQ WVRGWDDFK
Sbjct: 114 ENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHAAPETRAAAGQPWVRGWDDFK 173

Query: 67  RGRDGPITGNQ 78
           RGRD PI+GNQ
Sbjct: 174 RGRDAPISGNQ 184

BLAST of CmoCh04G020530 vs. TAIR 10
Match: AT5G51040.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 (InterPro:IPR005631). )

HSP 1 Score: 127.1 bits (318), Expect = 6.1e-30
Identity = 56/71 (78.87%), Postives = 61/71 (85.92%), Query Frame = 0

Query: 7   ENPDLWKWLTGQEQPPEAVKTNPVFTDVQEKVMNNLNKHASPETRTPPGQQWVRGWDDFK 66
           ENPDLWKWLT QEQPPEAV +NPVF+ + EKVM NLNKHA+PETR   GQ WVRGWDDFK
Sbjct: 127 ENPDLWKWLTEQEQPPEAVSSNPVFSALHEKVMKNLNKHAAPETRAAAGQPWVRGWDDFK 186

Query: 67  RGRDGPITGNQ 78
           RGRD PI+GNQ
Sbjct: 187 RGRDAPISGNQ 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FI448.6e-2978.87Succinate dehydrogenase assembly factor 2, mitochondrial OS=Arabidopsis thaliana... [more]
Match NameE-valueIdentityDescription
A0A6J1EQG77.5e-36100.00succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita moschata O... [more]
A0A6J1JZA02.2e-3598.59succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucurbita maxima OX=... [more]
A0A5D3C9Y97.1e-3494.37Succinate dehydrogenase assembly factor 2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3B4137.1e-3494.37succinate dehydrogenase assembly factor 2, mitochondrial OS=Cucumis melo OX=3656... [more]
A0A0A0LKF97.8e-3391.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G072460 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G51040.16.1e-3078.87unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 ... [more]
AT5G51040.26.1e-3078.87unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF339 ... [more]
AT5G51040.36.1e-3078.87unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..77
NoneNo IPR availablePANTHERPTHR12469PROTEIN EMI5 HOMOLOG, MITOCHONDRIALcoord: 7..77
NoneNo IPR availablePANTHERPTHR12469:SF5FLAVINATOR OF SUCCINATE DEHYDROGENASEcoord: 7..77

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G020530.1CmoCh04G020530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006121 mitochondrial electron transport, succinate to ubiquinone
biological_process GO:0034553 mitochondrial respiratory chain complex II assembly
biological_process GO:0018293 protein-FAD linkage
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005739 mitochondrion