CmoCh04G017120 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh04G017120
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pectate lyase
Location	Cmo_Chr04: 8678089 .. 8680294 (+)
RNA-Seq Expression	CmoCh04G017120
Synteny	CmoCh04G017120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAGCACCCACGAGCAGAGCTATGCTTGGGGTTCTGCTAACATTATCCTTGGTGGCTACTGCATATGCTGACACTGGAAACTTCGATGATTACTGGAAATCACGCGCCGCCAAGGCTGAGAAAGCCGCCGCCAAGGCCTTCGACCCTAATCCAGAGAACGTCACCGACATCCTCAATGCTGAAGTTGAGCAGTAAGTTGTTTTATTTTATTTTATTTTATTTTATTTTTTCAATCTTCAGGAGAAATTTTGTAGTAAGAACTCAGGGGAAATGAATTAATGGGATTGGAGTTTGATGCAGGGAATTGAGCGGAAGCAATGATACGAGAAGGAATTTGAAGAGATATACAGGGCCATGTCGAGCCACTAATCCCATAGACCGGTGCTGGAGATGCGACCCCAATTGGGCCAAAAACAGGAAGAAGCTGGCCGATTGTGTTCTGGGTTTTGGCTGGAAGACCATCGGCGGGAAGTTGGGTCCATTCTACGTGGTGAACGATTCGTCGGACTCGGATATCATGGAACCAAAACCTGGGACCCTCCGACATGCTGTGATCCAAAAGGGGCCGTTGTGGATCACATTCTCAACCAACATGGCCATTAGGCTAACTCAGGAGCTCATCGTGTCCAGCGACAAGACCATAGATGCAAGAGGCGCTAATGTTCAGATTGCTTATGGGGCTGGCATTACTCTTCAGTATGTACAAAATGTGATCATCCATGGGCTTCGTATCCATCACATCGTCATGGGTAGCGGCGGAATGATCAGGGACTCCGTGGACCATGTGGGTTTGAGGACAATGAGCGACGGCGATGGCATTTCAATTTTCGGTTCCTCCAACATTTGGATTGACCATGTTTCCATGTCCAATTGTCAAGATGGTCTAATTGATGCCATCATGGGATCCACTGCCATCACCATATCCAATAGCCATTTCACTCACCACAATGATGTAATGAAGCATGCTGAAACCATGTGACTGATGTTTTCATAAAAGAGTTTAAAAGTTGGGAGTTTTATGCAGGTGATGCTGTTCGGAGCAAGCGATGGGTACTCGAATGATCAGATAATGCAAGTGACAGTGGCATTCAATCACTTTGGTCAAGGGTTGGTGCAGAGGATGCCAAGGTGCCGATGGGGGTTCTTCCATGTCGTTAACAATGACTACACTCATTGGCTTATGTATGCCATAGGTGGTAGCCAGCATCCCATCATTATCAGCCAGGGCAATCGATTCATTGCTCCTCCAAATGTAAATGCTAAAGAGGTAATCAAGTCAAGTCAAATCAAATCAAATCAAATGTTTAATTTTCTCAAGAAACTAAAACTGGGTGTGAACCAACTCAGGTGACAAAGAGAGAGTACTCGCCGGAGAGTGTGTGGAAGACGTGGACATGGAGATCAGAGGGAGACTTGATGATGAACGGAGCATTCTTTGTTACCTCCGGCGACCAAAGGAAGAAACGACCTTTCTCAAAGATGGATATGATCTCGTACAGAGAAGGGACATACGTTACCAGAATGACACGTATGTCAGGTGCGCTTCCCTGCTTCATAGGCAGACCATGCTAAACTTCAACTCACACGTCGACGCAAACTCGCCGTCAACGAACAGATTTTTACTCTTTGGGTCACCGCTCAACTCACTGAGTATTAATTTTTTGGTTGGCTCGACTGGATTGGGTCTTTCTGGCGTTATCAAGATACAAGTGAACTTGTTTTCCTCTTTGTTCACTTCCTTATTTACCATAGCATTAATAAATATTTGTTATTATTGGACATTTTTTTTATGGGGAAATTTTGAAAGAATTTAACAAATGTGAAATAAAAGAAGGTAAATTTGACGTTTGACGTGCGTTCTAACACATAATGTTACAACTTTTCTTTTTTATTTTTACCTTTGGCAAAGTCTTAAAAATTCCTCCTGTAAATGTTGTAAACTGTAAAAAAAAAAAAAATGTTAGAACTTTAATTTTACCTTGGCAAGATCATAAAAATTCCTCCTGTAAATGTTGTGAACTGTACAAAAAAAAAAGTGAGCTACAAAAGAATGGATGATGAATGAACCGTGATTCTAAAGAAATAATCAGCCACAAGAAGCGACATAGTAGTGATTTATACGGGGCAAAATTTTGTTCCATGACCCTTACATATCATTGATTCTGTAGAATCTGGAGTTGAATTGAATTTCTCAATACTGTAG

mRNA sequence

ATGGTAGCACCCACGAGCAGAGCTATGCTTGGGGTTCTGCTAACATTATCCTTGGTGGCTACTGCATATGCTGACACTGGAAACTTCGATGATTACTGGAAATCACGCGCCGCCAAGGCTGAGAAAGCCGCCGCCAAGGCCTTCGACCCTAATCCAGAGAACGTCACCGACATCCTCAATGCTGAAGTTGAGCAGGAATTGAGCGGAAGCAATGATACGAGAAGGAATTTGAAGAGATATACAGGGCCATGTCGAGCCACTAATCCCATAGACCGGTGCTGGAGATGCGACCCCAATTGGGCCAAAAACAGGAAGAAGCTGGCCGATTGTGTTCTGGGTTTTGGCTGGAAGACCATCGGCGGGAAGTTGGGTCCATTCTACGTGGTGAACGATTCGTCGGACTCGGATATCATGGAACCAAAACCTGGGACCCTCCGACATGCTGTGATCCAAAAGGGGCCGTTGTGGATCACATTCTCAACCAACATGGCCATTAGGCTAACTCAGGAGCTCATCGTGTCCAGCGACAAGACCATAGATGCAAGAGGCGCTAATGTTCAGATTGCTTATGGGGCTGGCATTACTCTTCAGTATGTACAAAATGTGATCATCCATGGGCTTCGTATCCATCACATCGTCATGGGTAGCGGCGGAATGATCAGGGACTCCGTGGACCATGTGGGTTTGAGGACAATGAGCGACGGCGATGGCATTTCAATTTTCGGTTCCTCCAACATTTGGATTGACCATGTTTCCATGTCCAATTGTCAAGATGGTCTAATTGATGCCATCATGGGATCCACTGCCATCACCATATCCAATAGCCATTTCACTCACCACAATGATGTGATGCTGTTCGGAGCAAGCGATGGGTACTCGAATGATCAGATAATGCAAGTGACAGTGGCATTCAATCACTTTGGTCAAGGGTTGGTGCAGAGGATGCCAAGGTGCCGATGGGGGTTCTTCCATGTCGTTAACAATGACTACACTCATTGGCTTATGTATGCCATAGGTGGTAGCCAGCATCCCATCATTATCAGCCAGGGCAATCGATTCATTGCTCCTCCAAATGTAAATGCTAAAGAGGTGACAAAGAGAGAGTACTCGCCGGAGAGTGTGTGGAAGACGTGGACATGGAGATCAGAGGGAGACTTGATGATGAACGGAGCATTCTTTGTTACCTCCGGCGACCAAAGGAAGAAACGACCTTTCTCAAAGATGGATATGATCTCGTACAGAGAAGGGACATACGTTACCAGAATGACACGTATGTCAGAATCTGGAGTTGAATTGAATTTCTCAATACTGTAG

Coding sequence (CDS)

ATGGTAGCACCCACGAGCAGAGCTATGCTTGGGGTTCTGCTAACATTATCCTTGGTGGCTACTGCATATGCTGACACTGGAAACTTCGATGATTACTGGAAATCACGCGCCGCCAAGGCTGAGAAAGCCGCCGCCAAGGCCTTCGACCCTAATCCAGAGAACGTCACCGACATCCTCAATGCTGAAGTTGAGCAGGAATTGAGCGGAAGCAATGATACGAGAAGGAATTTGAAGAGATATACAGGGCCATGTCGAGCCACTAATCCCATAGACCGGTGCTGGAGATGCGACCCCAATTGGGCCAAAAACAGGAAGAAGCTGGCCGATTGTGTTCTGGGTTTTGGCTGGAAGACCATCGGCGGGAAGTTGGGTCCATTCTACGTGGTGAACGATTCGTCGGACTCGGATATCATGGAACCAAAACCTGGGACCCTCCGACATGCTGTGATCCAAAAGGGGCCGTTGTGGATCACATTCTCAACCAACATGGCCATTAGGCTAACTCAGGAGCTCATCGTGTCCAGCGACAAGACCATAGATGCAAGAGGCGCTAATGTTCAGATTGCTTATGGGGCTGGCATTACTCTTCAGTATGTACAAAATGTGATCATCCATGGGCTTCGTATCCATCACATCGTCATGGGTAGCGGCGGAATGATCAGGGACTCCGTGGACCATGTGGGTTTGAGGACAATGAGCGACGGCGATGGCATTTCAATTTTCGGTTCCTCCAACATTTGGATTGACCATGTTTCCATGTCCAATTGTCAAGATGGTCTAATTGATGCCATCATGGGATCCACTGCCATCACCATATCCAATAGCCATTTCACTCACCACAATGATGTGATGCTGTTCGGAGCAAGCGATGGGTACTCGAATGATCAGATAATGCAAGTGACAGTGGCATTCAATCACTTTGGTCAAGGGTTGGTGCAGAGGATGCCAAGGTGCCGATGGGGGTTCTTCCATGTCGTTAACAATGACTACACTCATTGGCTTATGTATGCCATAGGTGGTAGCCAGCATCCCATCATTATCAGCCAGGGCAATCGATTCATTGCTCCTCCAAATGTAAATGCTAAAGAGGTGACAAAGAGAGAGTACTCGCCGGAGAGTGTGTGGAAGACGTGGACATGGAGATCAGAGGGAGACTTGATGATGAACGGAGCATTCTTTGTTACCTCCGGCGACCAAAGGAAGAAACGACCTTTCTCAAAGATGGATATGATCTCGTACAGAGAAGGGACATACGTTACCAGAATGACACGTATGTCAGAATCTGGAGTTGAATTGAATTTCTCAATACTGTAG

Protein sequence

MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSESGVELNFSIL

Homology

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BLAST of CmoCh04G017120 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 9.5e-157
Identity = 268/421 (63.66%), Postives = 318/421 (75.53%), Query Frame = 0


Query: 17  SLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQEL--------S 76
           +++ +  A  G++D+ W+ RA +A++ A   ++P+PENVT   N ++   +        +
Sbjct: 19  TIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKELKKVKGT 78

Query: 77  GSNDTRRNL--KRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPF 136
            +N TRR L  K+YTGPC  TNPID+CWRCDPNWA NRKKLADC +GFG K IGGK G F
Sbjct: 79  HNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIGGKDGEF 138

Query: 137 YVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 196
           YVV D+SD D  +PKPGTLRHAVIQK PLWI F   M IRL QE+I+ SDKTIDARG NV
Sbjct: 139 YVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTIDARGVNV 198

Query: 197 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNI 256
            I  GAGITLQY++NVIIHGL IH IV G+GGM+RD+VDH+G+RT SDGDGISIFG+S I
Sbjct: 199 HITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISIFGASYI 258

Query: 257 WIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 316
           WIDHVSM  C DGLIDA+ GST ITISN HFT HN+VMLFGASD  S DQ+MQ+T+AFNH
Sbjct: 259 WIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQITLAFNH 318

Query: 317 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 376
           FG+ L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HP II QGNRFIAPP++  K+VTK
Sbjct: 319 FGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIFKKQVTK 378

Query: 377 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKM-DMISYREGTYVTRMTRM 427
           REY+PESVW  WTWRSEG+L MNGA+F  SGD         + D IS      VT MTR 
Sbjct: 379 REYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAEDVTWMTRF 438

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BLAST of CmoCh04G017120 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.1e-147
Identity = 256/415 (61.69%), Postives = 306/415 (73.73%), Query Frame = 0


Query: 13  LLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSND 72
           LL  +  A + A+   FD+YW+ ++  A+  A KA+ P+PE VT+  N  V     G N 
Sbjct: 11  LLFFASAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NS 70

Query: 73  TRRNLK-RYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVND 132
           TRRNL+    G C ATNPIDRCWRC  NW+ NRK L  CV GFG KT GG  G  YVV D
Sbjct: 71  TRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTD 130

Query: 133 SSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYG 192
            SD  + +PK GTLR  VIQ  PLWI F  +M IRL QELI+++DKTID RGANVQIA G
Sbjct: 131 PSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGG 190

Query: 193 AGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHV 252
           A +T+Q+V NVIIHG+ IH I  G GG+IRDS  H G+RT SDGDGISI GSSNIWIDHV
Sbjct: 191 AQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHV 250

Query: 253 SMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGL 312
           S++ C DGLID I+GSTAITISN H T H+DVML GASD Y+ D+IMQVTVAFNHFG+GL
Sbjct: 251 SLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGL 310

Query: 313 VQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSP 372
           VQRMPRCR+GF HVVNNDYTHW+MYA+GGSQHP IISQGNR+IAP    AKEVTKR+Y+ 
Sbjct: 311 VQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAE 370

Query: 373 ESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMS 427
            + W  WTW+S+GDL ++GAFFV SG   + + +SK D+I  + GT+V R+TR S
Sbjct: 371 PAEWSKWTWKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFS 423

[Close]

BLAST of CmoCh04G017120 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.3e-145
Identity = 256/451 (56.76%), Postives = 320/451 (70.95%), Query Frame = 0


Query: 12  VLLTLSLVATAYADTGN-FDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDI 71
           V+   SL+ T  A+  +  D+YW ++A +A K    A+ P+P            +N TD+
Sbjct: 12  VICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDV 71

Query: 72  LNAEVEQEL------------SGSNDTRRNL------------KRYTGPCRATNPIDRCW 131
              E E+ +            S +N TRR+L             + TGPC A+NPID+CW
Sbjct: 72  EGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCW 131

Query: 132 RCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGP 191
           RC P+WA+ RKKL  CV GFG++T GGK G  YVV    D D++ P+PGTLRHAVIQK P
Sbjct: 132 RCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEP 191

Query: 192 LWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVM 251
           LWI F  +M+IRL+QEL+++SDKTIDARGANV IAYGAGIT+QYV N+IIHGL +HHIV 
Sbjct: 192 LWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVK 251

Query: 252 GSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISN 311
            SGG+IRDS++H G R  +DGDGISIFG++NIW+DH+SMS CQDGLIDAIMGSTAITISN
Sbjct: 252 SSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISN 311

Query: 312 SHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 371
           SHFTHHNDVML GA +   +D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 312 SHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWE 371

Query: 372 MYAIGGSQHPIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFF 425
           +YAIGGSQ P I+S GNRFIAPP+  + +EVTKR+Y+ ES WK W WRSE D+ MN A+F
Sbjct: 372 LYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYF 431

[Close]

BLAST of CmoCh04G017120 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.0e-143
Identity = 248/412 (60.19%), Postives = 300/412 (72.82%), Query Frame = 0


Query: 29  FDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGS---NDTRRNL-------- 88
           FD YW  R + A K    ++DP+P NVT+  N  V   +  S   NDTRR L        
Sbjct: 34  FDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVRSGRK 93

Query: 89  -KRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSD 148
             + +G C A NPID CWRCD NWA NRKKLADCVLGFG +T GGK GP YVV D+SD+D
Sbjct: 94  THKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDASDND 153

Query: 149 IMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITL 208
           ++ PKPGTLRHAV + GPLWI F+ +M I+L QEL+++SDKTID RGA V I  GAG+TL
Sbjct: 154 LINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGAGLTL 213

Query: 209 QYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNC 268
           Q+V NVIIH + + HIV G+GG+IRDS  H+GLRT SDGDGIS+FG++NIWIDHVSM+ C
Sbjct: 214 QFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVSMTRC 273

Query: 269 QDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMP 328
            DG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D+ MQ+TVAFNHFG+ L QRMP
Sbjct: 274 ADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLEQRMP 333

Query: 329 RCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWK 388
           RCR+G  HVVNNDYTHW MYAIGG+ +P IISQGNRFIAPPN  AK++TKREY+P   WK
Sbjct: 334 RCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPYGEWK 393

Query: 389 TWTWRSEGDLMMNGAFFVTSG-----DQRKKRPFSKMDMISYREGTYVTRMT 424
           +W W+SEGD  +NGA+FV SG       + K P      I  + GT V ++T
Sbjct: 394 SWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLT 445

[Close]

BLAST of CmoCh04G017120 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.7e-142
Identity = 249/442 (56.33%), Postives = 311/442 (70.36%), Query Frame = 0


Query: 18  LVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDILNAE--- 77
           L+ T   +    D+YW  RA +A +   +A+  +P            +N TD+   E   
Sbjct: 18  LIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDG 77

Query: 78  -----------VEQELSGSNDTRRNLK--------RYTGPCRATNPIDRCWRCDPNWAKN 137
                      +E   S +N TRR+L+        +  GPC A+NPID+CWRC  +WAK 
Sbjct: 78  DAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKR 137

Query: 138 RKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNM 197
           RKKL  CV GFG +T GGK G  YVV  + D D++ PKPGTLRHAVIQK PLWI F  +M
Sbjct: 138 RKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDM 197

Query: 198 AIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDS 257
           +IRL QEL+++S KTIDARGANV +A+GAGIT+Q+V+NVIIHGL IHHI   SGGMIRDS
Sbjct: 198 SIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHISESSGGMIRDS 257

Query: 258 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDV 317
           VDH G+RT +DGDG+SI+GSSNIW+DH+SMS CQDGLIDAI+GST ITISNSHFTHHNDV
Sbjct: 258 VDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDV 317

Query: 318 MLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 377
           ML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ 
Sbjct: 318 MLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQG 377

Query: 378 PIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKK 425
           P I+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F  SG+ + K
Sbjct: 378 PTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYK 437

[Close]

BLAST of CmoCh04G017120 vs. ExPASy TrEMBL
Match: A0A6J1GZF5 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111458538 PE=3 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 1.1e-251
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
           AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420

Query: 421 RMTRMS 427
           RMTRMS
Sbjct: 421 RMTRMS 426

[Close]

BLAST of CmoCh04G017120 vs. ExPASy TrEMBL
Match: A0A6J1KK28 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493864 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.7e-249
Identity = 422/426 (99.06%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYAD GNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
           AEVEQELSGSNDTRRNLKRYTGPC ATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFS+MDMISY+EGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSRMDMISYKEGTYVT 420

Query: 421 RMTRMS 427
           RMTRMS
Sbjct: 421 RMTRMS 426

[Close]

BLAST of CmoCh04G017120 vs. ExPASy TrEMBL
Match: A0A5D3CZB5 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.7e-223
Identity = 374/424 (88.21%), Postives = 400/424 (94.34%), Query Frame = 0


Query: 4    PTSRAMLGVLLTLSLVA-TAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAE 63
            P + A L +LLTLSL+A TA+AD G+FDDYWK+RAA+AE+AAA A++PNPE + D LNAE
Sbjct: 925  PITMARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAE 984

Query: 64   VEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGK 123
            V Q LSGSN TRRNLK+YTGPCRATNPIDRCWRCDPNWA+NRKKLADCVLGFG KTIGGK
Sbjct: 985  VGQALSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGK 1044

Query: 124  LGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDAR 183
            LGP+YVVNDSSDSD+M PKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIV+SDKTIDAR
Sbjct: 1045 LGPYYVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDAR 1104

Query: 184  GANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFG 243
            GANVQIAYGAGITLQYV+NVIIHGLRIHHIV+G+GGMIRD+VDHVGLRTMSDGDGISIFG
Sbjct: 1105 GANVQIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFG 1164

Query: 244  SSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTV 303
            SSN+WIDHVSMSNCQDGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYSNDQIMQVTV
Sbjct: 1165 SSNVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTV 1224

Query: 304  AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAK 363
            AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AK
Sbjct: 1225 AFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAK 1284

Query: 364  EVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRM 423
            EVTKREYSPE VWK+WTWRSEGDLMMNGAFFVTSGDQRK+RPFS  DMISY+ GTYV RM
Sbjct: 1285 EVTKREYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRM 1344

Query: 424  TRMS 427
            TR S
Sbjct: 1345 TRFS 1348

[Close]

BLAST of CmoCh04G017120 vs. ExPASy TrEMBL
Match: A0A5A7STF1 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003980 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.8e-222
Identity = 373/420 (88.81%), Postives = 398/420 (94.76%), Query Frame = 0


Query: 8   AMLGVLLTLSLVA-TAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQE 67
           A L +LLTLSL+A TA+AD G+FDDYWK+RAA+AE+AAA A++PNPE + D LNAEV Q 
Sbjct: 2   ARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQA 61

Query: 68  LSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPF 127
           LSGSN TRRNLK+YTGPCRATNPIDRCWRCDPNWA+NRKKLADCVLGFG KTIGGKLGP+
Sbjct: 62  LSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGPY 121

Query: 128 YVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 187
           YVVNDSSDSD+M PKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIV+SDKTIDARGANV
Sbjct: 122 YVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGANV 181

Query: 188 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNI 247
           QIAYGAGITLQYV+NVIIHGLRIHHIV+G+GGMIRD+VDHVGLRTMSDGDGISIFGSSN+
Sbjct: 182 QIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSNV 241

Query: 248 WIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 307
           WIDHVSMSNCQDGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYSNDQIMQVTVAFNH
Sbjct: 242 WIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFNH 301

Query: 308 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 367
           FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTK
Sbjct: 302 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTK 361

Query: 368 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMS 427
           REYSPE VWK+WTWRSEGDLMMNGAFFVTSGDQRK+RPFS  DMISY+ GTYV RMTR S
Sbjct: 362 REYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRFS 421

[Close]

BLAST of CmoCh04G017120 vs. ExPASy TrEMBL
Match: A0A1S3BFK5 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489090 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.8e-222
Identity = 373/420 (88.81%), Postives = 398/420 (94.76%), Query Frame = 0


Query: 8   AMLGVLLTLSLVA-TAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQE 67
           A L +LLTLSL+A TA+AD G+FDDYWK+RAA+AE+AAA A++PNPE + D LNAEV Q 
Sbjct: 2   ARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQA 61

Query: 68  LSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPF 127
           LSGSN TRRNLK+YTGPCRATNPIDRCWRCDPNWA+NRKKLADCVLGFG KTIGGKLGP+
Sbjct: 62  LSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGPY 121

Query: 128 YVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 187
           YVVNDSSDSD+M PKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIV+SDKTIDARGANV
Sbjct: 122 YVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGANV 181

Query: 188 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNI 247
           QIAYGAGITLQYV+NVIIHGLRIHHIV+G+GGMIRD+VDHVGLRTMSDGDGISIFGSSN+
Sbjct: 182 QIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSNV 241

Query: 248 WIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 307
           WIDHVSMSNCQDGLIDAIMGSTAITISN HFTHHN+VMLFGASDGYSNDQIMQVTVAFNH
Sbjct: 242 WIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFNH 301

Query: 308 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 367
           FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTK
Sbjct: 302 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTK 361

Query: 368 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMS 427
           REYSPE VWK+WTWRSEGDLMMNGAFFVTSGDQRK+RPFS  DMISY+ GTYV RMTR S
Sbjct: 362 REYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRFS 421

[Close]

BLAST of CmoCh04G017120 vs. NCBI nr
Match: XP_022957045.1 (probable pectate lyase P59 [Cucurbita moschata])

HSP 1 Score: 878.6 bits (2269), Expect = 2.2e-251
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
           AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420

Query: 421 RMTRMS 427
           RMTRMS
Sbjct: 421 RMTRMS 426

[Close]

BLAST of CmoCh04G017120 vs. NCBI nr
Match: XP_022999568.1 (probable pectate lyase P59 [Cucurbita maxima])

HSP 1 Score: 871.3 bits (2250), Expect = 3.5e-249
Identity = 422/426 (99.06%), Postives = 424/426 (99.53%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYAD GNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
           AEVEQELSGSNDTRRNLKRYTGPC ATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFS+MDMISY+EGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSRMDMISYKEGTYVT 420

Query: 421 RMTRMS 427
           RMTRMS
Sbjct: 421 RMTRMS 426

[Close]

BLAST of CmoCh04G017120 vs. NCBI nr
Match: KAG7032192.1 (putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 870.2 bits (2247), Expect = 7.8e-249
Identity = 422/426 (99.06%), Postives = 423/426 (99.30%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYAD GNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
           AEVEQELSGSNDTRRNLKRYTGPC ATNPIDRCWRCDPNWAKNRKKLADCVLGFGW+TIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDI EPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420

Query: 421 RMTRMS 427
           RMTRMS
Sbjct: 421 RMTRMS 426

[Close]

BLAST of CmoCh04G017120 vs. NCBI nr
Match: XP_023537906.1 (probable pectate lyase P59 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870.2 bits (2247), Expect = 7.8e-249
Identity = 422/426 (99.06%), Postives = 423/426 (99.30%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYAD GNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
           AEVEQELSGSNDTRRNLKRY GPC ATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRD+VDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420

Query: 421 RMTRMS 427
           RMTRMS
Sbjct: 421 RMTRMS 426

[Close]

BLAST of CmoCh04G017120 vs. NCBI nr
Match: KAG6601412.1 (putative pectate lyase P59, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 868.6 bits (2243), Expect = 2.3e-248
Identity = 421/426 (98.83%), Postives = 422/426 (99.06%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYAD GNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120
           AEVEQELSGSNDTRRNLKRYTGPC ATNPIDRCWRCDPNWAKNRKKLADC LGFGW+TIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCALGFGWRTIG 120

Query: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDI EPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420

Query: 421 RMTRMS 427
           RMTRMS
Sbjct: 421 RMTRMS 426

[Close]

BLAST of CmoCh04G017120 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 9.1e-147
Identity = 256/451 (56.76%), Postives = 320/451 (70.95%), Query Frame = 0


Query: 12  VLLTLSLVATAYADTGN-FDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDI 71
           V+   SL+ T  A+  +  D+YW ++A +A K    A+ P+P            +N TD+
Sbjct: 12  VICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDV 71

Query: 72  LNAEVEQEL------------SGSNDTRRNL------------KRYTGPCRATNPIDRCW 131
              E E+ +            S +N TRR+L             + TGPC A+NPID+CW
Sbjct: 72  EGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCW 131

Query: 132 RCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGP 191
           RC P+WA+ RKKL  CV GFG++T GGK G  YVV    D D++ P+PGTLRHAVIQK P
Sbjct: 132 RCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEP 191

Query: 192 LWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVM 251
           LWI F  +M+IRL+QEL+++SDKTIDARGANV IAYGAGIT+QYV N+IIHGL +HHIV 
Sbjct: 192 LWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVK 251

Query: 252 GSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISN 311
            SGG+IRDS++H G R  +DGDGISIFG++NIW+DH+SMS CQDGLIDAIMGSTAITISN
Sbjct: 252 SSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISN 311

Query: 312 SHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 371
           SHFTHHNDVML GA +   +D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 312 SHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWE 371

Query: 372 MYAIGGSQHPIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFF 425
           +YAIGGSQ P I+S GNRFIAPP+  + +EVTKR+Y+ ES WK W WRSE D+ MN A+F
Sbjct: 372 LYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYF 431

[Close]

BLAST of CmoCh04G017120 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 510.4 bits (1313), Expect = 1.5e-144
Identity = 248/412 (60.19%), Postives = 300/412 (72.82%), Query Frame = 0


Query: 29  FDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGS---NDTRRNL-------- 88
           FD YW  R + A K    ++DP+P NVT+  N  V   +  S   NDTRR L        
Sbjct: 34  FDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVRSGRK 93

Query: 89  -KRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSD 148
             + +G C A NPID CWRCD NWA NRKKLADCVLGFG +T GGK GP YVV D+SD+D
Sbjct: 94  THKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDASDND 153

Query: 149 IMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITL 208
           ++ PKPGTLRHAV + GPLWI F+ +M I+L QEL+++SDKTID RGA V I  GAG+TL
Sbjct: 154 LINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGAGLTL 213

Query: 209 QYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNC 268
           Q+V NVIIH + + HIV G+GG+IRDS  H+GLRT SDGDGIS+FG++NIWIDHVSM+ C
Sbjct: 214 QFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVSMTRC 273

Query: 269 QDGLIDAIMGSTAITISNSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMP 328
            DG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D+ MQ+TVAFNHFG+ L QRMP
Sbjct: 274 ADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLEQRMP 333

Query: 329 RCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWK 388
           RCR+G  HVVNNDYTHW MYAIGG+ +P IISQGNRFIAPPN  AK++TKREY+P   WK
Sbjct: 334 RCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPYGEWK 393

Query: 389 TWTWRSEGDLMMNGAFFVTSG-----DQRKKRPFSKMDMISYREGTYVTRMT 424
           +W W+SEGD  +NGA+FV SG       + K P      I  + GT V ++T
Sbjct: 394 SWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLT 445

[Close]

BLAST of CmoCh04G017120 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.6e-143
Identity = 248/442 (56.11%), Postives = 311/442 (70.36%), Query Frame = 0


Query: 18  LVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDILNAE--- 77
           L+ T   +    D+YW  RA +A +   +A+  +P            +N TD+   E   
Sbjct: 18  LIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDG 77

Query: 78  -----------VEQELSGSNDTRRNLK--------RYTGPCRATNPIDRCWRCDPNWAKN 137
                      +E   S +N TRR+L+        +  GPC A+NPID+CWRC  +WAK 
Sbjct: 78  DAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKR 137

Query: 138 RKKLADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNM 197
           RKKL  CV GFG +T GGK G  YVV  + D D++ PKPGTLRHAVIQK PLWI F  +M
Sbjct: 138 RKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDM 197

Query: 198 AIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDS 257
           +IRL QEL+++S KTIDARGANV +A+GAGIT+Q+V+NV+IHGL IHHI   SGGMIRDS
Sbjct: 198 SIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISESSGGMIRDS 257

Query: 258 VDHVGLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDV 317
           VDH G+RT +DGDG+SI+GSSNIW+DH+SMS CQDGLIDAI+GST ITISNSHFTHHNDV
Sbjct: 258 VDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDV 317

Query: 318 MLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 377
           ML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ 
Sbjct: 318 MLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQG 377

Query: 378 PIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKK 425
           P I+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F  SG+ + K
Sbjct: 378 PTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYK 437

[Close]

BLAST of CmoCh04G017120 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 486.1 bits (1250), Expect = 2.9e-137
Identity = 247/442 (55.88%), Postives = 314/442 (71.04%), Query Frame = 0


Query: 1   MVAPTSRAMLGVLLTLSLVA-TAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDIL 60
           MV   S   + V L+L++V  +  A    +D+YW  R   A +   +++DPNPENVTD  
Sbjct: 1   MVNLGSYVFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHF 60

Query: 61  N--AEVEQELSG-SNDTRRNLK---------RYTGPCRATNPIDRCWRCDPNWAKNRKKL 120
           N  A +  E +G  N+TRR+L+         R  G   + N ID+CWR D NW KNRKKL
Sbjct: 61  NYHAALAMETTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKL 120

Query: 121 ADCVLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRL 180
           ADCVLGFG KT GGK GP YVV D SD+D+++PKPGT+RHAV +  PLWI F+ +M I+L
Sbjct: 121 ADCVLGFGRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKL 180

Query: 181 TQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHV 240
            QELI+++DKTID RGA + I  GAG+TLQ+V+NVIIH + I  I  G+GG+I DS  H 
Sbjct: 181 QQELIITNDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHF 240

Query: 241 GLRTMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFG 300
           GLRT+SDGDGI+IFG++N+WIDHVSM++C DG+IDAIMGSTAITISNSHFT H++VMLFG
Sbjct: 241 GLRTVSDGDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFG 300

Query: 301 ASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIII 360
            ++    D+ MQ+TVAFNHFG+ L QRMPR R+G  HVVNNDYTHW MYAIGG+ +P II
Sbjct: 301 GTNKDVIDKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTII 360

Query: 361 SQGNRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSG-----DQRKK 420
           SQGNRFIAPP  ++K+VTKREY+P   WK+W W+SE D  +NGA+FV SG         K
Sbjct: 361 SQGNRFIAPPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPK 420

Query: 421 RPFSKMDMISYREGTYVTRMTR 425
            P  +   I  + GT V R+T+
Sbjct: 421 NPIPRKFAIRPQPGTKVRRLTK 442

[Close]

BLAST of CmoCh04G017120 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 451.8 bits (1161), Expect = 6.1e-127
Identity = 218/347 (62.82%), Postives = 256/347 (73.78%), Query Frame = 0


Query: 51  NPENVTDILNAEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADC 110
           NP+ V D + A  E  +    + RR L  +T  C   NPID CWRCDPNW KNRK+LADC
Sbjct: 47  NPDEVADEVLALTEMSVRNHTE-RRKLGYFT--CGTGNPIDDCWRCDPNWHKNRKRLADC 106

Query: 111 VLGFGWKTIGGKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQE 170
            +GFG   IGG+ G FYVV D  D + + P+PGTLRHAVIQ  PLWI F  +M I+L QE
Sbjct: 107 GIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQLKQE 166

Query: 171 LIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLR 230
           LIV+S KTID RGANV IA G  IT+Q+V NVI+HGL IH        M+R S  H G R
Sbjct: 167 LIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSSETHFGWR 226

Query: 231 TMSDGDGISIFGSSNIWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNDVMLFGASD 290
           TM+DGD ISIFGSS++WIDH S+S+C DGL+DA+MGSTAITISN+H THHN+VML G SD
Sbjct: 227 TMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEVMLLGHSD 286

Query: 291 GYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQG 350
            Y  D+ MQVT+A+NHFG GL+QRMPRCR G+FHVVNNDYTHW MYAIGGS +P I SQG
Sbjct: 287 SYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 346

Query: 351 NRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSG 398
           NR+ AP N  AKEVTKR  +P S WK W WRSEGDL+ NGA+F +SG
Sbjct: 347 NRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSG 390

The following BLAST results are available for this feature:

BLAST of CmoCh04G017120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P15722	9.5e-157	63.66	Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1	[more]
P40973	3.1e-147	61.69	Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1	[more]
Q9SRH4	1.3e-145	56.76	Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1	[more]
Q9M9S2	2.0e-143	60.19	Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1	[more]
Q9LFP5	1.7e-142	56.33	Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1	[more]

BLAST of CmoCh04G017120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GZF5	1.1e-251	100.00	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111458538 PE=3 SV=1	[more]
A0A6J1KK28	1.7e-249	99.06	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493864 PE=3 SV=1	[more]
A0A5D3CZB5	2.7e-223	88.21	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 P...	[more]
A0A5A7STF1	1.8e-222	88.81	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003980 ...	[more]
A0A1S3BFK5	1.8e-222	88.81	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489090 PE=3 SV=1	[more]

BLAST of CmoCh04G017120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022957045.1	2.2e-251	100.00	probable pectate lyase P59 [Cucurbita moschata]	[more]
XP_022999568.1	3.5e-249	99.06	probable pectate lyase P59 [Cucurbita maxima]	[more]
KAG7032192.1	7.8e-249	99.06	putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023537906.1	7.8e-249	99.06	probable pectate lyase P59 [Cucurbita pepo subsp. pepo]	[more]
KAG6601412.1	2.3e-248	98.83	putative pectate lyase P59, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmoCh04G017120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01270.1	9.1e-147	56.76	Pectate lyase family protein	[more]
AT1G14420.1	1.5e-144	60.19	Pectate lyase family protein	[more]
AT5G15110.1	1.6e-143	56.11	Pectate lyase family protein	[more]
AT2G02720.1	2.9e-137	55.88	Pectate lyase family protein	[more]
AT1G04680.1	6.1e-127	62.82	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 336..355 score: 74.52 coord: 378..402 score: 51.81 coord: 234..255 score: 68.83 coord: 134..159 score: 60.44 coord: 110..127 score: 57.41 coord: 170..186 score: 69.75 coord: 314..333 score: 78.1
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 162..359 e-value: 6.8E-100 score: 347.8
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 172..353 e-value: 9.5E-20 score: 71.1
IPR007524	Pectate lyase, N-terminal	PFAM	PF04431	Pec_lyase_N	coord: 27..77 e-value: 2.6E-17 score: 62.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 87..429 e-value: 1.1E-109 score: 368.6
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 12..428
None	No IPR available	PANTHER	PTHR31683:SF132	PECTATE LYASE	coord: 12..428
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 76..414

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G017120.1	CmoCh04G017120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030570	pectate lyase activity