CmoCh04G005610 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh04G005610 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.3e-29
Identity = 88/253 (34.78%), Postives = 124/253 (49.01%), Query Frame = 0

Query: 38  GRDGLCLQTAPLSIY-YTPTKLTDCDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKP 97
           GRDG C +         TP +L+ C E +S+Q W    DGTI++     CL   S     
Sbjct: 323 GRDGFCAEVKNGDEKDGTPVQLSSCGE-QSNQQWTFSTDGTIQSLG--KCLTTSS----- 382

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLT---GKQGQYVSLEVDEGAPSQG 157
              +V++ +C        KW  + DGTI +  SGLVLT     +G  VSLE +  A  QG
Sbjct: 383 ---SVMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEGTLVSLEKNVHAARQG 442

Query: 158 WEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKR--QRWVLYGDGTIRGNG 217
           W       P    I  +  +CL+   G   + L  CS K   +  Q+W LYGDGTIR N 
Sbjct: 443 W-IVGNVEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVDQKWALYGDGTIRVNN 502

Query: 218 DSNVCLTSLLSSS---VVVSKCADMPQQRWALAADNTIGHPNTNLVMDARTFVLLSPLIL 277
           D ++C+TS   SS   +++ KC     QRW    D TI +P++ LVM      +    I+
Sbjct: 503 DRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNPDSKLVMHVDQNDVPLRKII 562

Query: 278 VAKRTGTYSQQWI 282
           ++  +GT +QQWI
Sbjct: 563 LSHPSGTSNQQWI 563

BLAST of CmoCh04G005610 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 3.3e-28
Identity = 81/258 (31.40%), Postives = 129/258 (50.00%), Query Frame = 0

Query: 35  HLVGRDGLCLQTA-PLSIYYTPTKLTDCDESESDQLWAIVDDGTIRADNDRNCLVPHSRG 94
           ++VGRDGLC+          TP +L  C  ++ +Q W    D TIR+     C+   + G
Sbjct: 311 NIVGRDGLCVDVRNGYDTDGTPLQLWPCG-TQRNQRWTFDSDDTIRSMG--KCMT--ANG 370

Query: 95  IKPIGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLTGKQG---QYVSLEVDEGAP 154
           +   G+N+V+ +C+    + +KW    DG+I +  SGLV+T  +      + LE +  A 
Sbjct: 371 LNN-GSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLEDNIYAA 430

Query: 155 SQGWEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGN 214
           SQGW  T    P  A+I  +  +CLQ       + +  C +    +Q+W LYGD TIR N
Sbjct: 431 SQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEAT-SLQQQWALYGDRTIRVN 490

Query: 215 GDSNVCLTSLLSSS---VVVSKCADMPQQRWALAADNTIGHPNTNLVMDARTFVLLSPLI 274
               +C+T+   +S   +++ KC  +P QRW   +D  I +P +  VMD R   +    I
Sbjct: 491 STRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVSLREI 550

Query: 275 LVAKRTGTYSQQWIVMSL 286
           ++   TG  +QQW+   L
Sbjct: 551 IIFPATGNPNQQWVTQVL 561

BLAST of CmoCh04G005610 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.9e-25
Identity = 86/251 (34.26%), Postives = 117/251 (46.61%), Query Frame = 0

Query: 38  GRDGLCLQTA-PLSIYYTPTKLTDCDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKP 97
           G DGLC+       I   P +L  C  +E +QLW    DGTIR      CL   S     
Sbjct: 327 GWDGLCVDVRYGHYIDGNPVQLRPCG-NECNQLWTFRTDGTIRWLG--KCLTASS----- 386

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLTGKQ---GQYVSLEVDEGAPSQG 157
              +V++ DC        KW  + DGTI +  SGLVLT  Q   G  +SLE +  A  QG
Sbjct: 387 ---SVMIYDCNTVPPEATKWVVSIDGTITNPHSGLVLTAPQAAEGTALSLENNIHAARQG 446

Query: 158 WEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDS 217
           W   +   P    I  +  +CL+       + L  C      +Q W LYGDGTIR N + 
Sbjct: 447 WTVGD-VEPLVTFIVGYKQMCLRENGENNFVWLEDCVLN-RVQQEWALYGDGTIRVNSNR 506

Query: 218 NVCLTS---LLSSSVVVSKCADMPQQRWALAADNTIGHPNTNLVMDARTFVLLSPLILVA 277
           ++C+TS     S  +V+ KC     QRW    + TI +PN  L+MD     +    I++ 
Sbjct: 507 SLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLLMDVAQRDVSLRKIILY 564

Query: 278 KRTGTYSQQWI 282
           + TG  +QQWI
Sbjct: 567 RPTGNPNQQWI 564

BLAST of CmoCh04G005610 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.2e-24
Identity = 92/253 (36.36%), Postives = 121/253 (47.83%), Query Frame = 0

Query: 38  GRDGLCLQTAP-LSIYYTPTKLTDCDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKP 97
           G DGLC+       I     +L  C  +E +QLW    DGTIR      CL   S     
Sbjct: 327 GWDGLCVDVRDGHYIDGNTVQLGPCG-NECNQLWTFRTDGTIRWLG--KCLTTSS----- 386

Query: 98  IGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVLTGKQ---GQYVSLEVDEGAPSQG 157
              +V++ DC        KW  + DGTI +  SGLVLT  Q   G  +SLE +  A  QG
Sbjct: 387 ---SVMIYDCNTVPPEATKWVVSTDGTITNPRSGLVLTAPQAAEGTALSLENNIHAARQG 446

Query: 158 WEATERPYPKAANIKWHNNLCL-QFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGNGD 217
           W   +   P    I  +  +CL + G+  F + L  C       Q W LYGDGTIR N +
Sbjct: 447 WTVGD-VEPLVTFIVGYKQMCLTENGENNF-VWLEDCVLN-RVEQEWALYGDGTIRVNSN 506

Query: 218 SNVCLTS---LLSSSVVVSKCADMPQQRWALAADNTIGHPNTNLVMD-ARTFVLLSPLIL 277
            ++C+TS     S  +V+ KC     QRW    + TI +PN  LVMD A++ V L  +IL
Sbjct: 507 RSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISNPNAKLVMDVAQSNVSLRKIIL 564

Query: 278 VAKRTGTYSQQWI 282
               TG  +QQWI
Sbjct: 567 YPP-TGNPNQQWI 564

BLAST of CmoCh04G005610 vs. ExPASy Swiss-Prot
Match: P82683 (Beta-galactoside-specific lectin 3 OS=Viscum album OX=3972 PE=1 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 3.8e-24
Identity = 78/255 (30.59%), Postives = 119/255 (46.67%), Query Frame = 0

Query: 36  LVGRDGLCLQTAPLSIYY-TPTKLTDC-DESESDQLWAIVDDGTIRADNDRNCLVPHSRG 95
           +VGRDGLC+       +   P +L+ C   ++ +QLW I  DGTIR++    CL  +   
Sbjct: 320 IVGRDGLCVDVRDGKFHNGNPIQLSPCKSNTDPNQLWTIRRDGTIRSNG--RCLTTYG-- 379

Query: 96  IKPIGTNVVLVDCTKEIRSDMKWTKTNDGTIHHDESGLVL---TGKQGQYVSLEVDEGAP 155
               G  V++ DC   +R    W    +GTI +  S LVL   +G  G  ++++    + 
Sbjct: 380 -YTAGVYVMIFDCNTAVREATLWQIWGNGTIINPRSNLVLGAASGSSGTTLTVQTQVYSL 439

Query: 156 SQGWEATERPYPKAANIKWHNNLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGN 215
            QGW A     P+   I    +LC++      S+ +  C S   + Q W LYGDG+IR  
Sbjct: 440 GQGWLAGNDTAPREVTIYGFRDLCMEANGA--SVWVETCGSS-TENQNWALYGDGSIRPK 499

Query: 216 GDSNVCLT----SLLSSSVVVSKCADMPQQRWALAADNTIGHPNTNLVMDARTFVLLSPL 275
            + + CLT    S+ +   +VS  A    QRW    + TI + N  LVMD          
Sbjct: 500 QNQDQCLTCQGDSVATVINIVSCSAGSSGQRWVFTNEGTILNLNNGLVMDVAQSNPSLRR 559

Query: 276 ILVAKRTGTYSQQWI 282
           I++   TG  +Q W+
Sbjct: 560 IIIYPATGNPNQMWL 566

BLAST of CmoCh04G005610 vs. ExPASy TrEMBL
Match: A0A6J1JI54 (ribosome-inactivating protein SNAI'-like OS=Cucurbita maxima OX=3661 GN=LOC111485998 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 2.7e-158
Identity = 272/282 (96.45%), Postives = 274/282 (97.16%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEKTRQVLLCMVVAFSLATHIG  LGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGTTLGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDES+SDQLWAIVDDGTIR DNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTI HDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPK ANIKWHNNLCLQFGDG
Sbjct: 121 DGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKY+K QRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRW L
Sbjct: 181 EFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWGL 240

Query: 241 AADNTIGHPNTNLVMDARTFVLLSPLILVAKRTGTYSQQWIV 283
           AADNTIGHPNTNLVMDARTFV LSPLILVAKRTGTYSQQWIV
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPLILVAKRTGTYSQQWIV 282

BLAST of CmoCh04G005610 vs. ExPASy TrEMBL
Match: A0A6J1FU34 (nigrin b-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 4.3e-95
Identity = 183/287 (63.76%), Postives = 215/287 (74.91%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGR HLVGRDGLCL T  L  YY PT L+ 
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRTHLVGRDGLCLDTMLLLGYYPPTHLSQ 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           C++ +S QLW+I++DGTIR  + R CLVP S+  + +G NVV +DC+KEIR DMKWTK  
Sbjct: 61  CEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNK 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLT K G+ V++EV+E  PSQ WEATE   P  ANIKW +NLCL+  +G
Sbjct: 121 DGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEG 180

Query: 181 EFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMPQQ 240
           E S + L+ C S+ DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD PQQ
Sbjct: 181 ENSYVTLNEC-SRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQ 240

Query: 241 RWALAADNTIGHPNTNLVMDARTFV-LLSPLILVAKRTGTYSQQWIV 283
           RW LA D+TI HPNTNLVMD R  V LL P+I+V K  G  SQ+W +
Sbjct: 241 RWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 286

BLAST of CmoCh04G005610 vs. ExPASy TrEMBL
Match: A0A6J1IYQ6 (ribosome-inactivating protein SNAI-like OS=Cucurbita maxima OX=3661 GN=LOC111481109 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 7.8e-89
Identity = 176/287 (61.32%), Postives = 206/287 (71.78%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEK RQVLLC+ +AFSLA  I A   YGAFGGR HLVGRDGLCL T  LS YY  T L+ 
Sbjct: 1   MEKMRQVLLCIALAFSLA--IAAPSEYGAFGGRTHLVGRDGLCLDTMLLSGYYPQTDLSQ 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           C   +  QLW I++DGTIRA + R CLVP ++ I P+G NVV++DCTKE+R DMKWTK  
Sbjct: 61  CQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDMKWTKKK 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLT K G+ V+LE +E APSQ WEATE  YP  ANIKW +NLCL+  +G
Sbjct: 121 DGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCLKSREG 180

Query: 181 EFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMPQQ 240
           E S + L+ C S+ DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD PQQ
Sbjct: 181 ENSYVTLNEC-SRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGALVVVSRCADKPQQ 240

Query: 241 RWALAADNTIGHPNTNLVMDA-RTFVLLSPLILVAKRTGTYSQQWIV 283
           RW LA DNTI HPNTNLVMD  R   LL P+I+V K  G  SQ+W +
Sbjct: 241 RWGLAEDNTINHPNTNLVMDVHREVPLLPPMIVVDKHDGAPSQRWSI 284

BLAST of CmoCh04G005610 vs. ExPASy TrEMBL
Match: A0A6J1FNV0 (nigrin b-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=3 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 1.1e-87
Identity = 172/289 (59.52%), Postives = 208/289 (71.97%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATH--IGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKL 60
           M+K  QVL C+ +AFSLATH  I AA GYG+FG R HLVGRDGLCL T  L  YY PT L
Sbjct: 1   MDKMTQVLFCIALAFSLATHTAIAAASGYGSFGSRTHLVGRDGLCLDTMLLLGYYPPTHL 60

Query: 61  TDCDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTK 120
           + C++ +S QLW+I++DGTIR  + R CLVP S+  + +G NVV +DC+KEIR DMKWTK
Sbjct: 61  SQCEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTK 120

Query: 121 TNDGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFG 180
             DGTIHHDESGLVLT K G+ V++EV+E  PSQ WEATE   P  ANIKW +NLCL+  
Sbjct: 121 NKDGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSI 180

Query: 181 DGEFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMP 240
           +GE S + L+ C S+ DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD P
Sbjct: 181 EGENSYVTLNEC-SRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKP 240

Query: 241 QQRWALAADNTIGHPNTNLVMDARTFV-LLSPLILVAKRTGTYSQQWIV 283
           QQRW LA D+TI HPNTNLVMD R  V LL P+I+V K  G  SQ+W +
Sbjct: 241 QQRWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 288

BLAST of CmoCh04G005610 vs. ExPASy TrEMBL
Match: A0A5A7T854 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 2.0e-60
Identity = 136/296 (45.95%), Postives = 183/296 (61.82%), Query Frame = 0

Query: 5   RQVLLCMVVAFSLATHIGAAL--------GYGAFGGRAHLVGRDGLCLQTAPLSIYYT-- 64
           ++++L ++VAFSL TH+  A+         YG   G  HLVGRDGLCL+ +P   +Y   
Sbjct: 2   KEIVLSIIVAFSLTTHLAMAVPSNYGYGGEYGGVPGATHLVGRDGLCLEMSPW--FYRGN 61

Query: 65  --PTKLTDCDE-SESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIR 124
             PT+L+ CDE  +  QLW +V DGTIR  ND+ CLV  +  +  +    V+ +C KE  
Sbjct: 62  NFPTRLSPCDEHKKQTQLWTVVGDGTIRPMNDKFCLV--ADVLVGVINKAVVRECAKESN 121

Query: 125 SDMKWTKTNDGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHN 184
           S+ +WT   DGTI H ESG+VLTG    YV+L+ ++  PSQ WEATE   P  ANI+W N
Sbjct: 122 SNKRWTHKKDGTITHVESGMVLTG-DSDYVTLQSNKNTPSQSWEATESSTPMIANIEWLN 181

Query: 185 NLCLQFGDGEFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTS--LLSSSVVVSK 244
           NLCLQ  D    +GL+ C ++ +K QRW LYGDGTIR N + N CLTS   L   +V+SK
Sbjct: 182 NLCLQSTDDSNHVGLNGC-NRENKDQRWALYGDGTIRQNVNRNYCLTSDEYLGRFIVMSK 241

Query: 245 CADMPQQRWALAA-DNTIGHPNTNLVMDAR--TFVLLSPLILVAKRTGTYSQQWIV 283
           C D PQQRW L A D+TI HPNT++VMD     +   S  ++  +R G+ SQ+W +
Sbjct: 242 CEDKPQQRWNLGAKDSTIDHPNTDMVMDVLFVPYYDFSSAVVTNRRDGSASQRWTI 291

BLAST of CmoCh04G005610 vs. NCBI nr
Match: XP_022988756.1 (ribosome-inactivating protein SNAI'-like [Cucurbita maxima])

HSP 1 Score: 567.8 bits (1462), Expect = 5.6e-158
Identity = 272/282 (96.45%), Postives = 274/282 (97.16%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEKTRQVLLCMVVAFSLATHIG  LGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGTTLGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDES+SDQLWAIVDDGTIR DNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRGDNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTI HDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPK ANIKWHNNLCLQFGDG
Sbjct: 121 DGTIRHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKVANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKY+K QRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRW L
Sbjct: 181 EFSLGLSACSSKYNKSQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWGL 240

Query: 241 AADNTIGHPNTNLVMDARTFVLLSPLILVAKRTGTYSQQWIV 283
           AADNTIGHPNTNLVMDARTFV LSPLILVAKRTGTYSQQWIV
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPLILVAKRTGTYSQQWIV 282

BLAST of CmoCh04G005610 vs. NCBI nr
Match: KAG6600357.1 (hypothetical protein SDJN03_05590, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 565.1 bits (1455), Expect = 3.6e-157
Identity = 270/282 (95.74%), Postives = 277/282 (98.23%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEKTRQV+LCMVVAFSLATHIG ALGYGAFGGRAHL+GRDGLCLQTAPLSIYYTPTKLT 
Sbjct: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDES+SDQLWAIVDDGTIRADNDR+CLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG
Sbjct: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL
Sbjct: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240

Query: 241 AADNTIGHPNTNLVMDARTFVLLSPLILVAKRTGTYSQQWIV 283
           AADNTIGHPNTNLVMDARTFV LSP++LVAKR GT SQ+WIV
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIV 282

BLAST of CmoCh04G005610 vs. NCBI nr
Match: KAG7031016.1 (hypothetical protein SDJN02_05055, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 562.4 bits (1448), Expect = 2.4e-156
Identity = 269/281 (95.73%), Postives = 275/281 (97.86%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEKTRQV+LCMVVAFSLATHI  ALGYGAFGGRAHL+GRDGLCLQTAPLSIYYTPTKLT 
Sbjct: 1   MEKTRQVVLCMVVAFSLATHISTALGYGAFGGRAHLLGRDGLCLQTAPLSIYYTPTKLTI 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDESESDQLWAIVDDGTIRADNDR+CLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN
Sbjct: 61  CDESESDQLWAIVDDGTIRADNDRSCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG
Sbjct: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL
Sbjct: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240

Query: 241 AADNTIGHPNTNLVMDARTFVLLSPLILVAKRTGTYSQQWI 282
           AADNTIGHPNTNLVMDARTFV LSP++LVAKR GT SQ+WI
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWI 281

BLAST of CmoCh04G005610 vs. NCBI nr
Match: XP_023513493.1 (ribosome-inactivating protein SNAI'-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 556.6 bits (1433), Expect = 1.3e-154
Identity = 267/282 (94.68%), Postives = 276/282 (97.87%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEKTRQV+LCMVVAFSLATHIG ALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD
Sbjct: 1   MEKTRQVVLCMVVAFSLATHIGTALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           CDES+SDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVL+DCTKEIRSDMKWTKTN
Sbjct: 61  CDESDSDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLIDCTKEIRSDMKWTKTN 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTI HD+SGLVLTGKQGQYV+LEVDEGAPSQGWEATERPYPKAANIKWH+NLCLQFGDG
Sbjct: 121 DGTIRHDKSGLVLTGKQGQYVTLEVDEGAPSQGWEATERPYPKAANIKWHDNLCLQFGDG 180

Query: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240
           EFSLGLSACSSKYDKRQRWVLYGDGTIR NGDSNVCLTSLLSSSVVVSKCADMPQQRWAL
Sbjct: 181 EFSLGLSACSSKYDKRQRWVLYGDGTIR-NGDSNVCLTSLLSSSVVVSKCADMPQQRWAL 240

Query: 241 AADNTIGHPNTNLVMDARTFVLLSPLILVAKRTGTYSQQWIV 283
           AADNTIGHPNTNLVMDARTFV LSP++LVAKR GT SQ+WIV
Sbjct: 241 AADNTIGHPNTNLVMDARTFVRLSPMVLVAKRAGTDSQRWIV 281

BLAST of CmoCh04G005610 vs. NCBI nr
Match: XP_022941585.1 (nigrin b-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 357.8 bits (917), Expect = 8.8e-95
Identity = 183/287 (63.76%), Postives = 215/287 (74.91%), Query Frame = 0

Query: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRAHLVGRDGLCLQTAPLSIYYTPTKLTD 60
           MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGR HLVGRDGLCL T  L  YY PT L+ 
Sbjct: 1   MEKTRQVLLCMVVAFSLATHIGAALGYGAFGGRTHLVGRDGLCLDTMLLLGYYPPTHLSQ 60

Query: 61  CDESESDQLWAIVDDGTIRADNDRNCLVPHSRGIKPIGTNVVLVDCTKEIRSDMKWTKTN 120
           C++ +S QLW+I++DGTIR  + R CLVP S+  + +G NVV +DC+KEIR DMKWTK  
Sbjct: 61  CEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNK 120

Query: 121 DGTIHHDESGLVLTGKQGQYVSLEVDEGAPSQGWEATERPYPKAANIKWHNNLCLQFGDG 180
           DGTIHHDESGLVLT K G+ V++EV+E  PSQ WEATE   P  ANIKW +NLCL+  +G
Sbjct: 121 DGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEG 180

Query: 181 EFS-LGLSACSSKYDKRQRWVLYGDGTIRGNGDSNVCLTSL---LSSSVVVSKCADMPQQ 240
           E S + L+ C S+ DK QRW LYGDGTIR N   ++CLTS      + VVVS+CAD PQQ
Sbjct: 181 ENSYVTLNEC-SRDDKNQRWALYGDGTIRQNEYRHLCLTSSERNYGAMVVVSRCADKPQQ 240

Query: 241 RWALAADNTIGHPNTNLVMDARTFV-LLSPLILVAKRTGTYSQQWIV 283
           RW LA D+TI HPNTNLVMD R  V LL P+I+V K  G  SQ+W +
Sbjct: 241 RWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 286