CmoCh04G001450 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh04G001450
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	gibberellin 2-beta-dioxygenase 2
Location	Cmo_Chr04: 763591 .. 765271 (-)
RNA-Seq Expression	CmoCh04G001450
Synteny	CmoCh04G001450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCGTGTTCTTGTTCACATCCTCTCAATGGCTTCCTCAGTTGATCACAATAAGTTACCAAACATCTGCGGCGGCGCCACCGCAGCTCCTCCGCCCACTCCTTCCTCACAAACCAACCACCTCTCCACCTCTGCCGCTGCCGAAGCTCTCTCCAGGCTCCTCCACCGCCTCCCCCCCAAACTCTCTCTTCCTACGCGCCGTTCCTCGTCCGTTATATCGCCACCAACGATTTCCTTCTCAGATTCTCCCGATGACGAGTTTTCCAGTAGGCTACTCTCCGCCGTCTCAGAATTCGGCTTCTTCCAAATCACTGATCATAAAATATCCTCTCATCTCGTTCTCTCAGCCGAGTCGGAATCTGCCGCTCTATTCGACATTCAGGCGGAGAAGAAGGAAGCACTGTTTCCGAAGGACTGGGGATTCATAGGCGACGAAGACGAAGAATCGGACGGAGCCGGTGAATCGTTCTGCTTCGATCCGAGGCTGTGCTTGCCTTATTCCGACGAGATTTGTTTACAAGCTTTGCAAGAGCTCGTTCCTGCGATGGAGTATCTCGGACTGAAGATCGTGGAGCACCTATTTCGTGCGATTGGCCTCGAGAATCCAATCGGTGAGGATCGAAGCAGATTCCGGTCGCTGATATGGATATCGAAAGGTTCTCGAAGCGCTGAGCCGGCAGTGACCTGTGGATTTTACCCATACATTGTTGGATTGCATTATCAGAGCAAGAACCAGAGGTGTTCTGTATTAGGAGATTCCGGCTGGGTTGCGACGGCGGCAACGGCGGCGGATTCAGTGATGGTTTCAATCGGAGATATTGCGCAAGTAAGAAATAAACGCTGTCGTTTTGTTAATTTAATTTCTTTTTTTAAAATGAATAGAAAATTATAATTAATGGGTAAGGATATTTGATAATTAATAATTAAAAAGCTGTTAGATTGCACGAACTGAGAGGCATTGGTCACGTGCGTTCATTCGTGACAAATATGCTGCTTGTTTCACAAATCCACACAAACCTAATTTCTCGAAATTATTGATTTAATATTCTAAAAAGAAATTATTAGAGGAAGAAAAATTTAGCATTAAAATTTTGAAAATGTATTTTTAAAATAAAATTATTTCCGATTGTTTATCAAAATTTGTAGGTGTGGAGCAATGGAAAGGTAAAGAAAGTGAGAGGCAGGGCAGTAGCGGCGGCGGCTGACGGCAGCGACAACTCGGGCACCATCTCAATATCACTACTCATTACTCTTCCTTTGGATACCCAAGTCTCTCCTCTTCTTCCACCGACCGACGAAAACACAGACGCAGAAGCAGAAGAGGACGGCGGCGGCGGCAACGAGGCAGAGAAGAAAACGGCGGTATTTCGTTCGTTTAGCTTTGAAGAGTACGCGTGGAAAGTGTATCAGAATCGGTGCTTTCTGCTCAGAGATCCGCTGGCCGGATACCGAATCTGAATCGATGGATCGGAGAATTGCCCCATTGTTGATCAATCGACCCATTGAATTCCCACGGTTTTCTGCAGAGGCGTTGAGTACGTAGCTCTACTTTTTCTTTTTTCTTGTTTATTTTGAACAGTTTTTGGATTATTCTTGCGAGTGCTGTGTGATTTTGTTACAAATTTGAATAATCCCAAAAACAATATATTTAGATATTATATGGATAATCAACTTCAGGTTCA

mRNA sequence

CTTCGTGTTCTTGTTCACATCCTCTCAATGGCTTCCTCAGTTGATCACAATAAGTTACCAAACATCTGCGGCGGCGCCACCGCAGCTCCTCCGCCCACTCCTTCCTCACAAACCAACCACCTCTCCACCTCTGCCGCTGCCGAAGCTCTCTCCAGGCTCCTCCACCGCCTCCCCCCCAAACTCTCTCTTCCTACGCGCCGTTCCTCGTCCGTTATATCGCCACCAACGATTTCCTTCTCAGATTCTCCCGATGACGAGTTTTCCAGTAGGCTACTCTCCGCCGTCTCAGAATTCGGCTTCTTCCAAATCACTGATCATAAAATATCCTCTCATCTCGTTCTCTCAGCCGAGTCGGAATCTGCCGCTCTATTCGACATTCAGGCGGAGAAGAAGGAAGCACTGTTTCCGAAGGACTGGGGATTCATAGGCGACGAAGACGAAGAATCGGACGGAGCCGGTGAATCGTTCTGCTTCGATCCGAGGCTGTGCTTGCCTTATTCCGACGAGATTTGTTTACAAGCTTTGCAAGAGCTCGTTCCTGCGATGGAGTATCTCGGACTGAAGATCGTGGAGCACCTATTTCGTGCGATTGGCCTCGAGAATCCAATCGGTGAGGATCGAAGCAGATTCCGGTCGCTGATATGGATATCGAAAGGTTCTCGAAGCGCTGAGCCGGCAGTGACCTGTGGATTTTACCCATACATTGTTGGATTGCATTATCAGAGCAAGAACCAGAGGTGTTCTGTATTAGGAGATTCCGGCTGGGTTGCGACGGCGGCAACGGCGGCGGATTCAGTGATGGTTTCAATCGGAGATATTGCGCAAGTGTGGAGCAATGGAAAGGTAAAGAAAGTGAGAGGCAGGGCAGTAGCGGCGGCGGCTGACGGCAGCGACAACTCGGGCACCATCTCAATATCACTACTCATTACTCTTCCTTTGGATACCCAAGTCTCTCCTCTTCTTCCACCGACCGACGAAAACACAGACGCAGAAGCAGAAGAGGACGGCGGCGGCGGCAACGAGGCAGAGAAGAAAACGGCGGTATTTCGTTCGTTTAGCTTTGAAGAGTACGCGTGGAAAGTGTATCAGAATCGGTGCTTTCTGCTCAGAGATCCGCTGGCCGGATACCGAATCTGAATCGATGGATCGGAGAATTGCCCCATTGTTGATCAATCGACCCATTGAATTCCCACGGTTTTCTGCAGAGGCGTTGAGTACGTAGCTCTACTTTTTCTTTTTTCTTGTTTATTTTGAACAGTTTTTGGATTATTCTTGCGAGTGCTGTGTGATTTTGTTACAAATTTGAATAATCCCAAAAACAATATATTTAGATATTATATGGATAATCAACTTCAGGTTCA

Coding sequence (CDS)

ATGGCTTCCTCAGTTGATCACAATAAGTTACCAAACATCTGCGGCGGCGCCACCGCAGCTCCTCCGCCCACTCCTTCCTCACAAACCAACCACCTCTCCACCTCTGCCGCTGCCGAAGCTCTCTCCAGGCTCCTCCACCGCCTCCCCCCCAAACTCTCTCTTCCTACGCGCCGTTCCTCGTCCGTTATATCGCCACCAACGATTTCCTTCTCAGATTCTCCCGATGACGAGTTTTCCAGTAGGCTACTCTCCGCCGTCTCAGAATTCGGCTTCTTCCAAATCACTGATCATAAAATATCCTCTCATCTCGTTCTCTCAGCCGAGTCGGAATCTGCCGCTCTATTCGACATTCAGGCGGAGAAGAAGGAAGCACTGTTTCCGAAGGACTGGGGATTCATAGGCGACGAAGACGAAGAATCGGACGGAGCCGGTGAATCGTTCTGCTTCGATCCGAGGCTGTGCTTGCCTTATTCCGACGAGATTTGTTTACAAGCTTTGCAAGAGCTCGTTCCTGCGATGGAGTATCTCGGACTGAAGATCGTGGAGCACCTATTTCGTGCGATTGGCCTCGAGAATCCAATCGGTGAGGATCGAAGCAGATTCCGGTCGCTGATATGGATATCGAAAGGTTCTCGAAGCGCTGAGCCGGCAGTGACCTGTGGATTTTACCCATACATTGTTGGATTGCATTATCAGAGCAAGAACCAGAGGTGTTCTGTATTAGGAGATTCCGGCTGGGTTGCGACGGCGGCAACGGCGGCGGATTCAGTGATGGTTTCAATCGGAGATATTGCGCAAGTGTGGAGCAATGGAAAGGTAAAGAAAGTGAGAGGCAGGGCAGTAGCGGCGGCGGCTGACGGCAGCGACAACTCGGGCACCATCTCAATATCACTACTCATTACTCTTCCTTTGGATACCCAAGTCTCTCCTCTTCTTCCACCGACCGACGAAAACACAGACGCAGAAGCAGAAGAGGACGGCGGCGGCGGCAACGAGGCAGAGAAGAAAACGGCGGTATTTCGTTCGTTTAGCTTTGAAGAGTACGCGTGGAAAGTGTATCAGAATCGGTGCTTTCTGCTCAGAGATCCGCTGGCCGGATACCGAATCTGA

Protein sequence

MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLLRDPLAGYRI

Homology

[Close]

BLAST of CmoCh04G001450 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 8.1e-08
Identity = 72/340 (21.18%), Postives = 140/340 (41.18%), Query Frame = 0


Query: 54  LPTRRSSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 113
           +PT +   V++  +I   +  D E  +R++ A  EFGFF++ +H +   L+   E E+  
Sbjct: 17  IPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 76

Query: 114 LFDI-QAEKKEALFPKDWGF----IGDEDEESDGAGESFCFDPRLCLP-------YSDEI 173
            F + Q+ K  A  P+ +G+    IG   +           +P+L  P        + +I
Sbjct: 77  FFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQI 136

Query: 174 CLQALQELVPAMEYLGLKIVEHLFRAIGLE------NPIGEDRS-RFRSLIWISKGSRSA 233
             ++++E +  ++ +  K++E +   +G+E        + +++S     L         A
Sbjct: 137 FRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEA 196

Query: 234 EPAVTCGF----YPYIVGLHYQSKNQRCSV-LGDSGWVATAATAADSVMVSIGDIAQVWS 293
           E  V  GF     P I+ +   +      + + D  WVA     + S  +++GD  QV +
Sbjct: 197 EKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHS-SFFINVGDALQVMT 256

Query: 294 NGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTDAEAEEDGGG 353
           NG+ K V+ R +A           IS+      PL  +++PL     E  D         
Sbjct: 257 NGRFKSVKHRVLA-----DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDD--------- 316

Query: 354 GNEAEKKTAVFRSFSFEEYAWKVYQNRCFLLRDPLAGYRI 370
                        + ++E+ W  Y++  +  +  L  YR+
Sbjct: 317 -------------WLYKEFTWSQYKSSAY--KSKLGDYRL 326

[Close]

BLAST of CmoCh04G001450 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 7.6e-06
Identity = 59/271 (21.77%), Postives = 107/271 (39.48%), Query Frame = 0


Query: 65  PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEA 124
           P  I   D  D +  ++++ A  EFGFF++ +H +   L+   E E+   F +    K+ 
Sbjct: 24  PVLIPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDK 83

Query: 125 LFPKD-WGF----IGDEDEESDGAGESFCFDPRLCLP---------YSDEICLQALQELV 184
             P D +G+    IG   +   G  E    +  LCL          ++  I  +A++E +
Sbjct: 84  AGPPDPFGYGTKRIGPNGDL--GWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYI 143

Query: 185 PAMEYLGLKIVEHLFRAIGLEN--------PIGEDRSRFRSLIWISKGSRSAEPAVTCGF 244
             M+ +  K +E +   + +E          + E  S  R   +  K     +  +  G 
Sbjct: 144 KEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVKEEIGFGE 203

Query: 245 Y--PYIVGLHYQSKNQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRA 304
           +  P ++ L   +  +   +    G          S  V +GD  QV +NG+ K V+ R 
Sbjct: 204 HTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRV 263

Query: 305 VAAAADGSDNSGTISISLLITLPLDTQVSPL 312
           V      +     IS+      PL  +++PL
Sbjct: 264 VT-----NTKRSRISMIYFAGPPLSEKIAPL 287

[Close]

BLAST of CmoCh04G001450 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.7e-05
Identity = 74/294 (25.17%), Postives = 119/294 (40.48%), Query Frame = 0


Query: 58  RSSSVIS----PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 117
           R SS +S      +I   D  + + ++ +  A   +G FQI +H IS  L+   ES S  
Sbjct: 35  RPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKT 94

Query: 118 LFDIQAEKKEALFPKDWGFIG----------DEDEESDG---AGESFCFDPRLCLPYSDE 177
           LFD+ +E+K      D G  G          ++   S+G   A +S+        P+   
Sbjct: 95  LFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHT 154

Query: 178 ICLQALQELVPAMEYLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISK----GSRSAEP 237
                +QE V  ME L  +++  +  ++G+             + W  K    GS+    
Sbjct: 155 KYCGIIQEYVDEMEKLASRLLYCILGSLGV---------TVEDIEWAHKLEKSGSKVGRG 214

Query: 238 AVTCGFYPY-----------------IVGLHYQSKNQRCSVL-GDSGWVATAATAADSVM 297
           A+    YP                  I+ + +QS      V   +SGWV T   A   ++
Sbjct: 215 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV-TVEPAPGVLV 274

Query: 298 VSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPL-DTQVSPL 312
           V+IGD+  + SNGK+  V  RA       +     ISI+ L   P  D Q++P+
Sbjct: 275 VNIGDLFHILSNGKIPSVVHRAKV-----NHTRSRISIAYLWGGPAGDVQIAPI 313

[Close]

BLAST of CmoCh04G001450 vs. ExPASy Swiss-Prot
Match: P07920 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Solanum lycopersicum OX=4081 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.4e-04
Identity = 71/304 (23.36%), Postives = 124/304 (40.79%), Query Frame = 0


Query: 81  RLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEALFPKDWGFIGDEDEES 140
           ++  A   +GFF++ +H I   ++ + E  +   +    E++        G  G E E +
Sbjct: 23  KINDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKCMEQRFKELVAKKGLEGVEVEVT 82

Query: 141 DGAGESFCFDPRL------CLPYSDEICLQALQELVPAMEYLGLKIVEHLFRAIGLENPI 200
           D   ES  F   L       LP  D++  + +++    +E L  ++++ L   +GLE   
Sbjct: 83  DMDWESTFFLRHLPSSNISQLPDLDDVYREVMRDFRKRLEKLAEELLDLLCENLGLE--- 142

Query: 201 GEDRSRFRSLIWISKGSR----------SAEPAVTCGFYPY-----IVGLHYQSKNQRCS 260
              +S  ++  + SKG              +P +  G   +     I+ L    K     
Sbjct: 143 ---KSYLKNTFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGIILLFQDDKVSGLQ 202

Query: 261 VLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLL 320
           +L D  W+        S++V++GD  +V +NGK K V  R V A  DG+     +S++  
Sbjct: 203 LLKDGRWI-DVPPMRHSIVVNLGDQLEVITNGKYKSVMHR-VIAQKDGT----RMSLASF 262

Query: 321 ITLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFL 364
                D     L+ P     D EAE         E    V+  F F++Y  K+Y N  F 
Sbjct: 263 YNPGNDA----LIYPAPALVDKEAE---------EHNKQVYPKFMFDDY-MKLYANLKFQ 300

[Close]

BLAST of CmoCh04G001450 vs. ExPASy TrEMBL
Match: A0A6J1FRA7 (gibberellin 3-beta-dioxygenase 4 OS=Cucurbita moschata OX=3662 GN=LOC111448055 PE=4 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 2.5e-206
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300

Query: 301 TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 301 TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 361 RDPLAGYRI 369

[Close]

BLAST of CmoCh04G001450 vs. ExPASy TrEMBL
Match: A0A6J1JL54 (uncharacterized protein LOC111485581 OS=Cucurbita maxima OX=3661 GN=LOC111485581 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.1e-200
Identity = 362/369 (98.10%), Postives = 363/369 (98.37%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 103 MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 162

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFS+SPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSA SESAALFDIQAE
Sbjct: 163 SVISPPTISFSESPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAVSESAALFDIQAE 222

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 223 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 282

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           VE LFRAIGLENPIGE RSRFRSLIW SKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 283 VEPLFRAIGLENPIGEGRSRFRSLIWTSKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 342

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 343 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 402

Query: 301 TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEAEED GGGNE EKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 403 TLPLDTQVSPLLPPTDENTDAEAEED-GGGNEVEKKTAVFRSFSFEEYAWKVYQNRCFLL 462

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 463 RDPLAGYRI 470

[Close]

BLAST of CmoCh04G001450 vs. ExPASy TrEMBL
Match: A0A1S3CNS4 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC103502873 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.9e-137
Identity = 268/383 (69.97%), Postives = 308/383 (80.42%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS++HN+LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALS+LLHRLPP LSLPTRRS 
Sbjct: 1   MASSLNHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVI+PPTISFS+SP+ +  + LLSA SE GFFQ+TDHKISSHL LSAESESAALF++ AE
Sbjct: 61  SVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSAE 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  GF GD DEESDG+G+S CFD RLC   S EI L +L E V  ME LGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGDSLCFDSRLCFSDSAEISLHSLTEFVLEMESLGL 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KIVE LFRAIG ENPIGEDR+ FRSL+WIS+G R  EPA+  GFYPYIVGL YQS+NQRC
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQSRNQRC 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAAD----GSDNSGTI 300
           S+LGDSGWVA AA AADSVMVSIGD+AQVWSNGK+KK+RGR V  A+      S NS TI
Sbjct: 241 SLLGDSGWVA-AAAAADSVMVSIGDVAQVWSNGKLKKMRGRPVPMASSLANTSSTNSRTI 300

Query: 301 SISLLITLPLDTQVSPLLPPTDENTDAE--------AEEDGGGGNEAEKKTAVFRSFSFE 360
           S+SLLITLP+DTQVSPLL  T+EN   E         +++    N+  K+ AVF SF+FE
Sbjct: 301 SLSLLITLPVDTQVSPLL-STNENEKEEQFASVRDKMKKEEDADNDEGKEKAVFHSFNFE 360

Query: 361 EYAWKVYQNRCFLLRDPLAGYRI 370
           EYAW+VY +RCFLL+DPL  YRI
Sbjct: 361 EYAWRVYPDRCFLLKDPLNRYRI 381

[Close]

BLAST of CmoCh04G001450 vs. ExPASy TrEMBL
Match: A0A6J1CYY0 (gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111015875 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.6e-123
Identity = 255/376 (67.82%), Postives = 288/376 (76.60%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDH ++     G TAAPPPTPSSQT+HLSTSAAA+ALSRLLHRLPP LSLPTRRS 
Sbjct: 1   MASSVDHKQIYG--AGTTAAPPPTPSSQTSHLSTSAAADALSRLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SV SPPTISFS++ +     RLLSA SE GFFQ+TDH+ISS L LSAESESAALF +  E
Sbjct: 61  SVRSPPTISFSETSNPSLLDRLLSASSELGFFQLTDHQISSRLALSAESESAALFGLPME 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGE----SFCFDPRLCLPYSDEICLQALQELVPAME 180
            KE+LFPK+W  GF GD DE+SDG+G     SFC D   C   S EI L +LQE V AME
Sbjct: 121 TKESLFPKNWPLGFKGDGDEDSDGSGNGDSFSFCLDSTPCSSDSAEISLHSLQEFVRAME 180

Query: 181 YLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSK 240
            LGLKIVE L RAIG ENPIGEDR+RFRSLIWIS+GSR AEPAV  GFYPYIVGL YQS+
Sbjct: 181 KLGLKIVEFLSRAIGFENPIGEDRTRFRSLIWISEGSRRAEPAVAGGFYPYIVGLQYQSR 240

Query: 241 NQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTI 300
           NQ+CS+LGDS WV TAA AADSVMVSIGDIAQVWSNGK+KKVRGR VA AAD   N G I
Sbjct: 241 NQKCSLLGDSSWV-TAAAAADSVMVSIGDIAQVWSNGKLKKVRGRPVAVAAD--PNGGGI 300

Query: 301 SISLLITLPLDTQVSPLL-PPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVY 360
           SISLLITLP +++V PL+ PP   +   EA E+     E E+    F+SF  EEYAW+V+
Sbjct: 301 SISLLITLPAESEVRPLITPPARSDRRDEAAEE-----EEEEVKMAFKSFWLEEYAWRVH 360

Query: 361 QNRCFLLRDPLAGYRI 370
            +RCFLL+DPL  YRI
Sbjct: 361 HDRCFLLKDPLDRYRI 366

[Close]

BLAST of CmoCh04G001450 vs. ExPASy TrEMBL
Match: A0A5A7UEE7 (1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold186G001390 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 6.7e-106
Identity = 204/268 (76.12%), Postives = 229/268 (85.45%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS++HN+LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALS+LLHRLPP LSLPTRRS 
Sbjct: 1   MASSLNHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVI+PPTISFS+SP+ +  + LLSA SE GFFQ+TDHKISSHL LSAESESAALF++ AE
Sbjct: 61  SVIAPPTISFSESPNPDLLNHLLSAASELGFFQLTDHKISSHLALSAESESAALFNLSAE 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  GF GD DEESDG+G+S CFD RLC   S EI L +L E V  ME LGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGDSLCFDSRLCFSDSAEISLHSLTEFVLEMESLGL 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KIVE LFRAIG ENPIGEDR+ FRSL+WIS+G R  EPA+  GFYPYIVGL YQS+NQRC
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRGTEPAMAGGFYPYIVGLQYQSRNQRC 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQ 267
           S+LGDSGWVA AA AADSVMVSIGD+AQ
Sbjct: 241 SLLGDSGWVA-AAAAADSVMVSIGDVAQ 267

[Close]

BLAST of CmoCh04G001450 vs. NCBI nr
Match: XP_022943276.1 (gibberellin 3-beta-dioxygenase 4 [Cucurbita moschata] >KAG6599990.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030665.1 Gibberellin 2-beta-dioxygenase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 727.6 bits (1877), Expect = 5.3e-206
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300

Query: 301 TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 301 TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 361 RDPLAGYRI 369

[Close]

BLAST of CmoCh04G001450 vs. NCBI nr
Match: XP_023524652.1 (gibberellin 3-beta-dioxygenase 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 710.3 bits (1832), Expect = 8.7e-201
Identity = 363/369 (98.37%), Postives = 366/369 (99.19%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHL+LSAESESAALFDIQAE
Sbjct: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLLLSAESESAALFDIQAE 120

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGA ESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 121 KKEALFPKDWGFIGDEDEESDGACESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           +E LFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 181 IEPLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300

Query: 301 TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEA+ED GGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 301 TLPLDTQVSPLLPPTDENTDAEAKED-GGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 361 RDPLAGYRI 368

[Close]

BLAST of CmoCh04G001450 vs. NCBI nr
Match: XP_022988289.1 (uncharacterized protein LOC111485581 [Cucurbita maxima])

HSP 1 Score: 706.4 bits (1822), Expect = 1.3e-199
Identity = 362/369 (98.10%), Postives = 363/369 (98.37%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS
Sbjct: 103 MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 162

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVISPPTISFS+SPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSA SESAALFDIQAE
Sbjct: 163 SVISPPTISFSESPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAVSESAALFDIQAE 222

Query: 121 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 180
           KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI
Sbjct: 223 KKEALFPKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKI 282

Query: 181 VEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 240
           VE LFRAIGLENPIGE RSRFRSLIW SKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV
Sbjct: 283 VEPLFRAIGLENPIGEGRSRFRSLIWTSKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSV 342

Query: 241 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 300
           LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI
Sbjct: 343 LGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLI 402

Query: 301 TLPLDTQVSPLLPPTDENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLL 360
           TLPLDTQVSPLLPPTDENTDAEAEED GGGNE EKKTAVFRSFSFEEYAWKVYQNRCFLL
Sbjct: 403 TLPLDTQVSPLLPPTDENTDAEAEED-GGGNEVEKKTAVFRSFSFEEYAWKVYQNRCFLL 462

Query: 361 RDPLAGYRI 370
           RDPLAGYRI
Sbjct: 463 RDPLAGYRI 470

[Close]

BLAST of CmoCh04G001450 vs. NCBI nr
Match: XP_038892860.1 (gibberellin 2-beta-dioxygenase 6 [Benincasa hispida])

HSP 1 Score: 506.9 bits (1304), Expect = 1.5e-139
Identity = 271/379 (71.50%), Postives = 303/379 (79.95%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS+DHN+LPNI GGATAAPPPTPSSQTNHLSTSAAA+ALSRLLHRLPP LSLPTRR  
Sbjct: 16  MASSLDHNQLPNIHGGATAAPPPTPSSQTNHLSTSAAADALSRLLHRLPPNLSLPTRRFP 75

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SV SPPTISFS+SP+ +   RLLSA SE GFFQ+TDHKISSHL LSAESE+AALFD+ AE
Sbjct: 76  SVRSPPTISFSESPNPDLLDRLLSAASELGFFQLTDHKISSHLALSAESEAAALFDLPAE 135

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  G+ GD DEESDG GESFCFD R CL  S EI L +L+E V  ME LGL
Sbjct: 136 KKESLFPKNWPLGYKGDGDEESDGIGESFCFDLRPCLSDSAEISLHSLKEFVLKMESLGL 195

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KI+E LFRAIG ENPIGEDR+ FRSLIWIS+G RS EPA+  GFYPYIVGL YQSKNQRC
Sbjct: 196 KILEFLFRAIGFENPIGEDRTGFRSLIWISEGCRSTEPAMADGFYPYIVGLQYQSKNQRC 255

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKV-KKVRGRAVAAAADGSDNSGTISIS 300
           S+LGDSGWVA AA A DSVMVSIGDIAQVWSNGK+ KKVRGR V  A     NS  IS+S
Sbjct: 256 SLLGDSGWVAAAAAATDSVMVSIGDIAQVWSNGKLPKKVRGRPVPTANTNGANSHAISLS 315

Query: 301 LLITLPLDTQVSPLLPPTDENTDAE-------AEEDGGGGNEAEKKTAVFRSFSFEEYAW 360
           LLITLP+D+QVSPLL P+  N D E        ++     N+  K+ A+F SFSFEEYAW
Sbjct: 316 LLITLPVDSQVSPLLLPSSVNADVEQFRSVTAKKKKEDDDNDERKEKAMFHSFSFEEYAW 375

Query: 361 KVYQNRCFLLRDPLAGYRI 370
           +VY +RCFLL+DPL  YRI
Sbjct: 376 RVYHDRCFLLKDPLNRYRI 394

[Close]

BLAST of CmoCh04G001450 vs. NCBI nr
Match: XP_011654997.1 (gibberellin 2-beta-dioxygenase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 504.2 bits (1297), Expect = 9.5e-139
Identity = 271/381 (71.13%), Postives = 307/381 (80.58%), Query Frame = 0


Query: 1   MASSVDHNKLPNICGGATAAPPPTPSSQTNHLSTSAAAEALSRLLHRLPPKLSLPTRRSS 60
           MASS+DHN+LPNI  GATAAPPPTPSSQTNHLSTSAAA+ALS+LLHRLPP LSLPTRRS 
Sbjct: 1   MASSLDHNQLPNIHAGATAAPPPTPSSQTNHLSTSAAADALSKLLHRLPPNLSLPTRRSP 60

Query: 61  SVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 120
           SVI PPTISFS+SP+ +  +RLLSA SE GFFQ+TDHKISSHL LSAESESA LF++ AE
Sbjct: 61  SVIPPPTISFSESPNPDLLNRLLSAASELGFFQLTDHKISSHLALSAESESAPLFNLPAE 120

Query: 121 KKEALFPKDW--GFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGL 180
           KKE+LFPK+W  GF GD DEESDG+GES CFD R CL  S EI   +L + V  ME LGL
Sbjct: 121 KKESLFPKNWPLGFKGDGDEESDGSGESLCFDSRNCLSDSPEISFHSLTDFVLEMESLGL 180

Query: 181 KIVEHLFRAIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRC 240
           KIVE LFRAIG ENPIGEDR+ FRSL+WIS+G RS EPA+  GFYPYI+GL YQS+NQ+C
Sbjct: 181 KIVEFLFRAIGFENPIGEDRTGFRSLVWISEGCRSTEPAMAGGFYPYIIGLQYQSRNQKC 240

Query: 241 SVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAAD----GSDNSGTI 300
           S+LGDSGWVA AA AADSVMVSIGDIAQVWSNGK+KK+RGR V  A+      S NS TI
Sbjct: 241 SLLGDSGWVA-AAAAADSVMVSIGDIAQVWSNGKLKKMRGRPVPMASSVANTSSTNSRTI 300

Query: 301 SISLLITLPLDTQVSPLLPPTDENTD------AEAEEDGGGGNEAEKKTAVFRSFSFEEY 360
           S+SLLITLP+DTQVSPLL  T+EN +       E E+DG  G   EK  AVF SF+FEEY
Sbjct: 301 SLSLLITLPVDTQVSPLLLSTNENANEEQFDKKEREDDGDSGEGKEK--AVFHSFNFEEY 360

Query: 361 AWKVYQNRCFLLRDPLAGYRI 370
           AW+VY +RCFLL+DPL  YRI
Sbjct: 361 AWRVYHDRCFLLKDPLNRYRI 378

[Close]

BLAST of CmoCh04G001450 vs. TAIR 10
Match: AT3G11150.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.5e-65
Identity = 160/367 (43.60%), Postives = 221/367 (60.22%), Query Frame = 0


Query: 15  GGATAAPPPTPSSQTNHLSTSA----AAEALSRLLHRLPPKLSLPTRRSSSVISPPTISF 74
           GGATAAPPPTPS   +HL+T++    A +ALS L HRLPP LSLP RR  S+ S P +S 
Sbjct: 18  GGATAAPPPTPS--CSHLNTTSKSETAVDALSSLFHRLPPLLSLPNRR--SIPSLPMVSL 77

Query: 75  SDSPDDEFSSRLLSAVSEFGFFQI----TDHKISSHLVLSAESESAALFDIQAEKKEALF 134
           S     E+   L+SAV++FG+FQ+    +D      +  +AES+S +L ++  EKKE+ F
Sbjct: 78  SAGDRLEWDD-LISAVTDFGYFQLINDDSDILFPPGIAEAAESDSLSLLELSEEKKESSF 137

Query: 135 PKDWGFIGDEDEESDGAGESFCFDPRLCLPYSDEICLQALQELVPAMEYLGLKIVEHLFR 194
           PK W        E+D    SFC D   C   S E+ L +L+E    +E +GLK VE L  
Sbjct: 138 PKKWPL----GYEADAETPSFCLDAD-CSTDSSELNLSSLREFTRTLEKVGLKTVEMLAN 197

Query: 195 AIGLENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYPYIVGLHYQSKNQRCSVLGDSGW 254
           A+G     G D +RF +L+W+++G    EP VT GFYP++V L YQ + Q+  +L +SGW
Sbjct: 198 ALG----FGYDSTRFNTLMWVNQGVPDDEPEVTNGFYPFVVCLQYQIREQKYCLLTESGW 257

Query: 255 VATAATAADSVMVSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDT 314
           V +     DSV+V++GDIAQVW NG+VK+V+ R V  +         ++++L++TLP+D+
Sbjct: 258 V-SVLPRVDSVLVTLGDIAQVWRNGEVKRVKYRPVLCSGQKDGPVKCVTMTLMLTLPMDS 317

Query: 315 QVSPLLPPTD----ENTDAEAEEDGGGGNEAEKKTAVFRSFSFEEYAWKVYQNRCFLLRD 370
            VS L         E   AE EE+ GG    E++   FR FSF+EYAW+VYQ R F  RD
Sbjct: 318 MVSSLKDMISDGDKEEEYAEEEEEDGGARSDERR--AFRPFSFKEYAWRVYQERLF-FRD 366

[Close]

BLAST of CmoCh04G001450 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 59.7 bits (143), Expect = 5.7e-09
Identity = 72/340 (21.18%), Postives = 140/340 (41.18%), Query Frame = 0


Query: 54  LPTRRSSSVISPPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 113
           +PT +   V++  +I   +  D E  +R++ A  EFGFF++ +H +   L+   E E+  
Sbjct: 17  IPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 76

Query: 114 LFDI-QAEKKEALFPKDWGF----IGDEDEESDGAGESFCFDPRLCLP-------YSDEI 173
            F + Q+ K  A  P+ +G+    IG   +           +P+L  P        + +I
Sbjct: 77  FFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQI 136

Query: 174 CLQALQELVPAMEYLGLKIVEHLFRAIGLE------NPIGEDRS-RFRSLIWISKGSRSA 233
             ++++E +  ++ +  K++E +   +G+E        + +++S     L         A
Sbjct: 137 FRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAEEEA 196

Query: 234 EPAVTCGF----YPYIVGLHYQSKNQRCSV-LGDSGWVATAATAADSVMVSIGDIAQVWS 293
           E  V  GF     P I+ +   +      + + D  WVA     + S  +++GD  QV +
Sbjct: 197 EKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHS-SFFINVGDALQVMT 256

Query: 294 NGKVKKVRGRAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTDAEAEEDGGG 353
           NG+ K V+ R +A           IS+      PL  +++PL     E  D         
Sbjct: 257 NGRFKSVKHRVLA-----DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDD--------- 316

Query: 354 GNEAEKKTAVFRSFSFEEYAWKVYQNRCFLLRDPLAGYRI 370
                        + ++E+ W  Y++  +  +  L  YR+
Sbjct: 317 -------------WLYKEFTWSQYKSSAY--KSKLGDYRL 326

[Close]

BLAST of CmoCh04G001450 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 53.1 bits (126), Expect = 5.4e-07
Identity = 59/271 (21.77%), Postives = 107/271 (39.48%), Query Frame = 0


Query: 65  PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAEKKEA 124
           P  I   D  D +  ++++ A  EFGFF++ +H +   L+   E E+   F +    K+ 
Sbjct: 24  PVLIPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDK 83

Query: 125 LFPKD-WGF----IGDEDEESDGAGESFCFDPRLCLP---------YSDEICLQALQELV 184
             P D +G+    IG   +   G  E    +  LCL          ++  I  +A++E +
Sbjct: 84  AGPPDPFGYGTKRIGPNGDL--GWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYI 143

Query: 185 PAMEYLGLKIVEHLFRAIGLEN--------PIGEDRSRFRSLIWISKGSRSAEPAVTCGF 244
             M+ +  K +E +   + +E          + E  S  R   +  K     +  +  G 
Sbjct: 144 KEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEETPVKEEIGFGE 203

Query: 245 Y--PYIVGLHYQSKNQRCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRGRA 304
           +  P ++ L   +  +   +    G          S  V +GD  QV +NG+ K V+ R 
Sbjct: 204 HTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRV 263

Query: 305 VAAAADGSDNSGTISISLLITLPLDTQVSPL 312
           V      +     IS+      PL  +++PL
Sbjct: 264 VT-----NTKRSRISMIYFAGPPLSEKIAPL 287

[Close]

BLAST of CmoCh04G001450 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 52.0 bits (123), Expect = 1.2e-06
Identity = 74/294 (25.17%), Postives = 119/294 (40.48%), Query Frame = 0


Query: 58  RSSSVIS----PPTISFSDSPDDEFSSRLLSAVSEFGFFQITDHKISSHLVLSAESESAA 117
           R SS +S      +I   D  + + ++ +  A   +G FQI +H IS  L+   ES S  
Sbjct: 35  RPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKT 94

Query: 118 LFDIQAEKKEALFPKDWGFIG----------DEDEESDG---AGESFCFDPRLCLPYSDE 177
           LFD+ +E+K      D G  G          ++   S+G   A +S+        P+   
Sbjct: 95  LFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGFTIADDSYRNHFNTLWPHDHT 154

Query: 178 ICLQALQELVPAMEYLGLKIVEHLFRAIGLENPIGEDRSRFRSLIWISK----GSRSAEP 237
                +QE V  ME L  +++  +  ++G+             + W  K    GS+    
Sbjct: 155 KYCGIIQEYVDEMEKLASRLLYCILGSLGV---------TVEDIEWAHKLEKSGSKVGRG 214

Query: 238 AVTCGFYPY-----------------IVGLHYQSKNQRCSVL-GDSGWVATAATAADSVM 297
           A+    YP                  I+ + +QS      V   +SGWV T   A   ++
Sbjct: 215 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV-TVEPAPGVLV 274

Query: 298 VSIGDIAQVWSNGKVKKVRGRAVAAAADGSDNSGTISISLLITLPL-DTQVSPL 312
           V+IGD+  + SNGK+  V  RA       +     ISI+ L   P  D Q++P+
Sbjct: 275 VNIGDLFHILSNGKIPSVVHRAKV-----NHTRSRISIAYLWGGPAGDVQIAPI 313

[Close]

BLAST of CmoCh04G001450 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 4.5e-06
Identity = 66/282 (23.40%), Postives = 122/282 (43.26%), Query Frame = 0


Query: 62  VISPPTISFSDSPDDEFS-SRLLSAVSEFGFFQITDHKISSHLVLSAESESAALFDIQAE 121
           VI    +S  D+ D  F   +L  A  ++GFFQ+ +H I   +V   E  ++  FD+  E
Sbjct: 57  VIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMPLE 116

Query: 122 KKEALFPKDWG--------FIGDEDEESD-------GAGESFCFDPRLCLPYSDEICLQA 181
           +K+  +P + G        FI  ED++ D       G       +P+L  P       ++
Sbjct: 117 EKKK-YPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKL-WPSKPARFSES 176

Query: 182 LQELVPAMEYLGLKIVEHLFRAIGL-ENPIGEDRSRFRSLIWISKGSRSAEPAVTCGFYP 241
           L+     +  L  ++++++  ++GL E    E        + ++     + P +  G  P
Sbjct: 177 LEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLSP 236

Query: 242 YIVG----LHYQSKNQ--RCSVLGDSGWVATAATAADSVMVSIGDIAQVWSNGKVKKVRG 301
           +  G    +  QSKN      +L D+ WV       ++++++IGD  +V SNGK K V  
Sbjct: 237 HSDGSALTVLQQSKNSCVGLQILKDNTWV-PVKPLPNALVINIGDTIEVLSNGKYKSVEH 296

Query: 302 RAVAAAADGSDNSGTISISLLITLPLDTQVSPLLPPTDENTD 321
           RAV      +     ++I        + ++ P+    D+ T+
Sbjct: 297 RAVT-----NREKERLTIVTFYAPNYEVEIEPMSELVDDETN 330

The following BLAST results are available for this feature:

BLAST of CmoCh04G001450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XFR9	8.1e-08	21.18	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
O64692	7.6e-06	21.77	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
Q9C971	1.7e-05	25.17	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
P07920	2.4e-04	23.36	1-aminocyclopropane-1-carboxylate oxidase 2 OS=Solanum lycopersicum OX=4081 GN=A...	[more]

BLAST of CmoCh04G001450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRA7	2.5e-206	100.00	gibberellin 3-beta-dioxygenase 4 OS=Cucurbita moschata OX=3662 GN=LOC111448055 P...	[more]
A0A6J1JL54	6.1e-200	98.10	uncharacterized protein LOC111485581 OS=Cucurbita maxima OX=3661 GN=LOC111485581...	[more]
A0A1S3CNS4	1.9e-137	69.97	1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo OX=3656 GN=LOC10350287...	[more]
A0A6J1CYY0	1.6e-123	67.82	gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111015875 ...	[more]
A0A5A7UEE7	6.7e-106	76.12	1-aminocyclopropane-1-carboxylate oxidase OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of CmoCh04G001450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943276.1	5.3e-206	100.00	gibberellin 3-beta-dioxygenase 4 [Cucurbita moschata] >KAG6599990.1 Gibberellin ...	[more]
XP_023524652.1	8.7e-201	98.37	gibberellin 3-beta-dioxygenase 4 [Cucurbita pepo subsp. pepo]	[more]
XP_022988289.1	1.3e-199	98.10	uncharacterized protein LOC111485581 [Cucurbita maxima]	[more]
XP_038892860.1	1.5e-139	71.50	gibberellin 2-beta-dioxygenase 6 [Benincasa hispida]	[more]
XP_011654997.1	9.5e-139	71.13	gibberellin 2-beta-dioxygenase 2 isoform X1 [Cucumis sativus]	[more]

BLAST of CmoCh04G001450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11150.1	2.5e-65	43.60	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G30040.1	5.7e-09	21.18	gibberellin 2-oxidase	[more]
AT2G34555.1	5.4e-07	21.77	gibberellin 2-oxidase 3	[more]
AT1G80330.1	1.2e-06	25.17	gibberellin 3-oxidase 4	[more]
AT3G21420.1	4.5e-06	23.40	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 27..325 e-value: 3.9E-22 score: 81.2
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 66..128 e-value: 1.5E-8 score: 35.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 311..333
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 21..35
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..35
None	No IPR available	PANTHER	PTHR34945	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 1..369
None	No IPR available	PANTHER	PTHR34945:SF2	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 1..369
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 49..354

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G001450.1	CmoCh04G001450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity