CmoCh04G001160 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh04G001160
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	staphylococcal-like nuclease CAN2
Location	Cmo_Chr04: 604076 .. 607329 (-)
RNA-Seq Expression	CmoCh04G001160
Synteny	CmoCh04G001160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAGTCCAATTTATCAAACCATTTCATTACCTTAGATTCGCCATTGTTTAAGGCAGCAAAAAATGGGGAACGTTCTCAGGTTCCTCTGCGGTCACTTCTGCAATCCGAATGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCGCCGGCCACCGTCGGCGTCTCGGCTCTAGCTCACGATCTCTTTGATTTCGAGATCACCTCGCAGGTATGTTCTCAATCTGAAGCTCATCTCGAGCTGCATTTCTGGTTGATTGAATTTTGAATCGGATTCTGTCTGGTGATCTAGTGAAGTTTGTTGTTCAAACGATCTATGCTTTGGTATTGATGATTTTGATTTGATGAATTTTTGAAAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGGCGAATTGGTAAAGTATTTCTGATTGTTTGGTTACATAGTGGAGATTTGTGAAAGTAATTGTAGTTACAGAGTGGAGAGATTCTGATTGTTTGTGATGATCTGTGTGTGTGTGAAGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTCGAGGTACAAATAGGAATTCTCTTGCTTGTTTTGTTGGATTGGTAAACTGGGAATCTCTGCAGGTTAAATAGTTCAATTGATAAGAGTCAAAGTTCATTCTGTCAAATATGAGCAGCTCTGATGATAATAAGGAAATGCGTTGTTCTTTCCATTTCGAATTCAAATTTTTAGGAGAGAGAAATCAGATCCATGGGGACTTAGCTAGATGGCTTGTGTAGAACAGTCCAAAATCCAGTGGGGTTGGATCTGATGAACTGAAATGGAAAACAGATTACTTTTTGAAAAGTGTCAAATTACTTTTTGAAAATAGTGTTTTCAAGAACGTTTATGTTTTTAAAAAATAGTTAAATGCTTCCTTTAAAAGCATTTAAGTACTTTCTTTTCATTTTTAAAACTTCTTAACCACTTTTGTTCTTGCAAAATCAAGCTATAAGTTAAAAAAAAAAAAAAAAAATACAACAACAATTTTAGTCCTCCATTAGCATTTTAAAAGGAATGTCAAACACACTCAATCTAAGTTCTTTGTTTTTATTTCTGTTGTTCAACAGGGATTATTGGCTTTCTATGGTCTACCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCAGTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTAAGTGAGAAGGATGCTTTTATGGGAAATGACCAACCATTTTGTTCACAACAGGTGTTCTCATTTCTCAATGTTGAATTCATATCAGGTCGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTAGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCCATTCAAAGATCACAAGCTAGAAACGCCAAGAACTACGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGTAAATTATATCTCTGGTCTACTGCATACATACACTTTGATTTTTCCATAAGTTAGTGGGATTTGATACCTGTTTCTTCTCCCACAAAAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGGTAATTAAAATTAGTTTTTTTTCTTTCATTTTCGTGTATACAATTACAAATTGTTTATTTTAGGATTTGTACTTTCAACTTTTGGTCTATTTTGATTCATCTAATTTTAAAATGTCTATTTTAGTCTTTATACTATTTAGAAGTTCACACATTTATATTTTCTCATATTTTTTTTTCATCTCAATTTCATAGCAAAACACTACATTCCAAATTTACTAGAAATACTCTCTTTTAACTTTTATTAGATGATGATTATTGTGCATAGAATCCAGGATTTGCAAATAGACTAAATATTCAGTTTTCAAGAGCACAAAATCAAAATACGATTTAAACCTGAATAAAAATAGTGATTGTTCCATACTTGAACGATTTCCAAACTCCATTGACCCTTAGGCTTAGTTGAACTTTGATAATGCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTTGAAGGCAAGTGTTTAAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGTAACACTAATTACACAATTTCCACTTTCCTCGATGGATCATATGGGTTCATGTTTCTGGTTATGTAAGAATAAGTTTTTGGTTCTCTCTACAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGAGCAGAGCTGGCAAAGGTGCGTTACATTTTTATTTCATTCGTTAAACTTACATCCCATAAACTTCAAGTAACACTTCTGATCCTTGAGATTTAAATTAAACTTCTGTTTGGTCCCTAAAGTTTGAACTAGATTTGTAATTAATTCTGAAATTTGAAATGCTAACGTGATAGTTAAATATACTTAACTTGCCTCTGCCTTTGATGGCATGTATGTTTTAAATGAATAAAATATATATTATGTTTTATAAACGTACCAATTATAAATCAATTTCACATCCACCTCAACATTTTGGAAAAAAGAATTACAATTCAGACAGATATTGTCACATTCAAGTTTACAAACAGTTTTAACAGAGGGAAAGTGTTACGTGTATGCTACTTTACATGATCACTTTACTCAATTCTAAATTTCTAATGGATTCTAATTCATGATTCATAACAGTGGGAAAATGAAGCTCGAGCTAAAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAGTATACACAATTTATTTCAAGTCTCTACCAAGAAGAAGAGGAAGGAGACCCAAAACCAACCCGAACAAGGTTTCTTCTGTCTTTAGTATTTATCAACATCTTCAACATTGCTTGTAAATTTGTAACAGAAAATTTGACAAGAAATAGATTATTGTTATACTTTGTAAGACCATATCATTCACTTTTAAAGTTTAATCAGCCACTACAGCATGAGCATGAGGAAAGGTAGAATACAATGGATAACCATAAGCTTTACATGCC

mRNA sequence

TTCAGTCCAATTTATCAAACCATTTCATTACCTTAGATTCGCCATTGTTTAAGGCAGCAAAAAATGGGGAACGTTCTCAGGTTCCTCTGCGGTCACTTCTGCAATCCGAATGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCGCCGGCCACCGTCGGCGTCTCGGCTCTAGCTCACGATCTCTTTGATTTCGAGATCACCTCGCAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGGCGAATTGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTCGAGGGATTATTGGCTTTCTATGGTCTACCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCAGTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTCGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTAGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCCATTCAAAGATCACAAGCTAGAAACGCCAAGAACTACGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTTGAAGGCAAGTGTTTAAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGAGCAGAGCTGGCAAAGTGGGAAAATGAAGCTCGAGCTAAAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAGTATACACAATTTATTTCAAGTCTCTACCAAGAAGAAGAGGAAGGAGACCCAAAACCAACCCGAACAAGGTTTCTTCTGTCTTTAGTATTTATCAACATCTTCAACATTGCTTGTAAATTTGTAACAGAAAATTTGACAAGAAATAGATTATTGTTATACTTTGTAAGACCATATCATTCACTTTTAAAGTTTAATCAGCCACTACAGCATGAGCATGAGGAAAGGTAGAATACAATGGATAACCATAAGCTTTACATGCC

Coding sequence (CDS)

ATGGGGAACGTTCTCAGGTTCCTCTGCGGTCACTTCTGCAATCCGAATGCTGCCGCCGACGCCGATTCCCTCGGTCCTCATGGCGTCTCGCCGGCCACCGTCGGCGTCTCGGCTCTAGCTCACGATCTCTTTGATTTCGAGATCACCTCGCAGGTTCCTCAAGGACTTAGTAAGCATGTGGTTTCGTCGAAGAAGGCACAGGCGAATTGGTATAGAAAACTGGCTGAAGCTTGGAGGTCGGAGAAACCACCTCCTAAAACGCCTGAAGAAGCATCCAGGCTTGTGATTCAGACACTCAAGAGACACCAGAAGAAAGATGTCGAGGGATTATTGGCTTTCTATGGTCTACCTCTTCCTCATACTCTTGTCGAACTTTCTGCTCCGGCCCCAGTGCCTACATCGCTACCAGATGGAGTCAAGTTTGAGCTGCAGACATTGCCAGTCGATGCAAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGTACAGCACACCCTAGAGAGTCCTCTTGTATTCCAATGGAGGTTAGGAACGCAGCCATTCAAAGATCACAAGCTAGAAACGCCAAGAACTACGCAAAGGCTGATGCACTTCACAAGACAATAACTGATGCTGGTTACAGGGTGCTTACTGTTCAGAATGAGGAGATTCTTGCAAGAAAATACAGGATTCGTCTCAGAGGAATAGATGCACCAGAAAGCGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAGCTGAGAAAGCTTGTTGAAGGCAAGTGTTTAAGAGTACTTGTGTACGGAGAAGATCGGTATAACCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGCTGAAAAAGGGCTTTGCGTGGCATTACACTGCCTATGACAAACGAGCAGAGCTGGCAAAGTGGGAAAATGAAGCTCGAGCTAAAAGGATAGGGTTGTGGGCTTCTAGAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAAGGGAAATAG

Protein sequence

MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHVVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPHTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK

Homology

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BLAST of CmoCh04G001160 vs. ExPASy Swiss-Prot
Match: F4IH31 (Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 1.5e-133
Identity = 237/334 (70.96%), Postives = 272/334 (81.44%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN L FL G  C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQANWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  APV  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLRGIDAPES MP+
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPF 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA+E L K+V  K L+VLVYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKR
Sbjct: 241 GKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKR 335
             LAKWE EAR KRIGLWAS NPE+PW+WRK  R
Sbjct: 301 PVLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

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BLAST of CmoCh04G001160 vs. ExPASy Swiss-Prot
Match: F4IZC5 (Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 6.0e-127
Identity = 226/336 (67.26%), Postives = 263/336 (78.27%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN +R L               L  HGVS ++ GVSAL+ DL +FE TSQVP+ L  +V
Sbjct: 1   MGNAIRLL------------RKCLNSHGVSASSGGVSALSRDLLNFETTSQVPEKLGSYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSS+KAQANWYRK+ EAW+  KP PKTPEEASRLVI  LK HQK DVEGLL+FYGLP PH
Sbjct: 61  VSSQKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
            LVE+  P   P SLP GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +P +V  
Sbjct: 121 NLVEV--PTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA++R++AR  KNY +ADALHKTI  +GYR+++ QNEE+LA+K+RIRL GID+PES MPY
Sbjct: 181 AAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA +EL K+VEGKCL+VLVY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR
Sbjct: 241 GKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREG 337
           AELAKWENEAR KR+GLWAS NPE+PWEWRK KR G
Sbjct: 301 AELAKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

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BLAST of CmoCh04G001160 vs. ExPASy Swiss-Prot
Match: Q39635 (Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-126
Identity = 227/338 (67.16%), Postives = 264/338 (78.11%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFL GH C P           +GVS ATVGVSALAHDLF FE TSQ+P+GL+K+V
Sbjct: 1   MGNALRFLYGHCCKPTVEDHYQPPHGYGVSTATVGVSALAHDLFHFENTSQIPEGLTKYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWY+KL++AWR  KPPP+T E+A+RL+I TLKRHQK DV+GLL FYGLPL +
Sbjct: 61  VSSKKAQTNWYKKLSQAWREAKPPPQTAEQATRLIILTLKRHQKADVKGLLRFYGLPLSN 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
                +     P     GVKFEL TLPVD KAVADGDTVTVYV+T  PRE+S +P  V+ 
Sbjct: 121 NPSTEATTVVAPPQADQGVKFELHTLPVDVKAVADGDTVTVYVNTEDPREASNLPKSVKV 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLT-VQNEEILARKYRIRLRGIDAPESAMP 240
           AA +R++AR  ++Y KADAL K I DAGYRVL+   NE+ILARKYRIRLRGID+PES+MP
Sbjct: 181 AAQERAKARAVRDYVKADALQKNIVDAGYRVLSGPNNEDILARKYRIRLRGIDSPESSMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           +GKEAKEEL KLV GKCL V +Y EDRY R VGD+YCNG+FIQE MLKKG AWHYTAYDK
Sbjct: 241 FGKEAKEELIKLVVGKCLTVHIYEEDRYGRSVGDIYCNGQFIQEKMLKKGLAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R +L+KWE +ARA R+GLWAS NPE+PWEWRK KR GK
Sbjct: 301 RPQLSKWEEKARAARVGLWASSNPEKPWEWRKNKRNGK 338

[Close]

BLAST of CmoCh04G001160 vs. ExPASy Swiss-Prot
Match: Q942N7 (Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0166100 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 9.7e-117
Identity = 211/338 (62.43%), Postives = 252/338 (74.56%), Query Frame = 0


Query: 1   MGN-VLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN + RFLCG  C+P     +    PHG  PA   V+AL  D+  FE TSQVP GLS+H
Sbjct: 1   MGNSIYRFLCG-LCSP-----SPEYQPHGAHPA---VAALGRDIQQFEATSQVPDGLSRH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY+KL   W+  +P P+TPEEA+RLV+ TLK HQK DVEG L FYGLP+P
Sbjct: 61  VVSSKKAQANWYKKLIVTWKKARPTPRTPEEAARLVVTTLKNHQKADVEGFLVFYGLPIP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           +      AP       P G KFEL TLPVDAKAVADGDT+TVY+ TA PRES  +P E++
Sbjct: 121 NAAASTPAPHTAHVPKPQGCKFELHTLPVDAKAVADGDTITVYIDTADPRESGNVPREIQ 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYR-VLTVQNEEILARKYRIRLRGIDAPESAM 240
            AA +R++AR A++Y KAD L K I DAGYR V   + EE+LA+KYRIRLRGIDAPESAM
Sbjct: 181 KAAAERTRARAARDYQKADGLQKMIADAGYRQVPNARGEEVLAKKYRIRLRGIDAPESAM 240

Query: 241 PYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYD 300
           PYGKEAKE L K+V+GK L+V VY EDRY RCVGD+YC+G F+QE MLKKG AWHYTAYD
Sbjct: 241 PYGKEAKEALLKMVQGKSLKVYVYDEDRYGRCVGDIYCDGVFVQEQMLKKGCAWHYTAYD 300

Query: 301 KRAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREG 337
           +R ELAKWE +A++ R GLWA+  P++PWEWR+ KR G
Sbjct: 301 QRPELAKWEKQAQSGRKGLWAASRPQKPWEWRRDKRNG 329

[Close]

BLAST of CmoCh04G001160 vs. ExPASy Swiss-Prot
Match: Q2RBJ2 (Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0109500 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.0e-98
Identity = 173/323 (53.56%), Postives = 231/323 (71.52%), Query Frame = 0


Query: 15  PNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHVVSSKKAQANWYRKL 74
           P A      LGPHGV+P+TVGV+ALAHDL +FE TS VP GLS+HVVSS+KAQ  WY+KL
Sbjct: 41  PPAPLQQQHLGPHGVTPSTVGVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKWYQKL 100

Query: 75  AEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPHTLVELSAPAPVPTS 134
            EA+++  PPPKTP  A++L+ + L   Q+ D+EG+L FY LP+P   +  ++    P+ 
Sbjct: 101 LEAYKNTTPPPKTPANAAQLIARALNMIQRADLEGILEFYNLPIPS--LPTASSNYQPSL 160

Query: 135 LPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRNAAIQRSQARNAKNY 194
           LP+GV+F L TLPV  K + DGD  T YV T  PRES+ +P+EV    I R+QAR  ++Y
Sbjct: 161 LPEGVQFVLNTLPVYDKCIGDGDGFTAYVPTTDPRESANVPLEVHELVIARTQARKCRDY 220

Query: 195 AKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPYGKEAKEELRKLVEG 254
             ADAL  ++ +AGY++++  ++E+LARKYRIR+RGIDAPE  MPYGKE++  L KL+ G
Sbjct: 221 QSADALLSSLDEAGYKIISCSDDEVLARKYRIRMRGIDAPELKMPYGKESRTALVKLIGG 280

Query: 255 KCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRAELAKWENEARAKR 314
           K +++ VY  D++ R VGD+YCN  FIQE MLK G AWH+  YDKR E A+WE EARA  
Sbjct: 281 KSVKIYVYDLDQFGRYVGDIYCNNLFIQEQMLKNGHAWHFKTYDKRPEFARWEREARAAN 340

Query: 315 IGLWASRNPEQPWEWRKGKREGK 338
            GLWAS NPE+PW+WR+ +R  +
Sbjct: 341 RGLWASGNPEKPWDWRRDQRNAR 361

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BLAST of CmoCh04G001160 vs. ExPASy TrEMBL
Match: A0A6J1FPN5 (staphylococcal-like nuclease CAN2 OS=Cucurbita moschata OX=3662 GN=LOC111447091 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 8.6e-193
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmoCh04G001160 vs. ExPASy TrEMBL
Match: A0A6J1K113 (staphylococcal-like nuclease CAN2 OS=Cucurbita maxima OX=3661 GN=LOC111491421 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.8e-190
Identity = 332/337 (98.52%), Postives = 334/337 (99.11%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFLC HFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAP+PTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARA+RIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmoCh04G001160 vs. ExPASy TrEMBL
Match: A0A6J1CZ71 (staphylococcal-like nuclease CAN2 OS=Momordica charantia OX=3673 GN=LOC111015935 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 7.8e-170
Identity = 303/343 (88.34%), Postives = 315/343 (91.84%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAA-----DADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60
           MGN LRFLCGHFC+P+A +      +DSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG
Sbjct: 1   MGNALRFLCGHFCSPSAGSGGGGGGSDSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60

Query: 61  LSKHVVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120
           LSKHVVSSKKAQANWY KLAEAWRSEKPPP+TPEEASRLVIQTLKRHQKKDVEGLLAFYG
Sbjct: 61  LSKHVVSSKKAQANWYGKLAEAWRSEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120

Query: 121 LPLPHTLVELSAPAPVP-TSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCI 180
           LPLPHTLVELSA AP     LPDG+KFELQTLPVDAKAVADGDTVTVYVST +PRES+CI
Sbjct: 121 LPLPHTLVELSASAPTTGAPLPDGIKFELQTLPVDAKAVADGDTVTVYVSTENPRESACI 180

Query: 181 PMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAP 240
           P EV+ AA+QRS AR AKNYAKADALHK ITDAGYRVLT QNEEILA+KYRIRLRGIDAP
Sbjct: 181 PKEVQAAAVQRSHARAAKNYAKADALHKKITDAGYRVLTFQNEEILAKKYRIRLRGIDAP 240

Query: 241 ESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300
           ES+MPYGKEAKEEL KLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY
Sbjct: 241 ESSMPYGKEAKEELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300

Query: 301 TAYDKRAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
            AYDKRAELAKWE EARAKR GLWAS NPEQPWEWRK KREGK
Sbjct: 301 AAYDKRAELAKWEKEARAKRAGLWASSNPEQPWEWRKAKREGK 343

[Close]

BLAST of CmoCh04G001160 vs. ExPASy TrEMBL
Match: A0A5D3DQ96 (Staphylococcal-like nuclease CAN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold436G00570 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.7e-167
Identity = 291/338 (86.09%), Postives = 314/338 (92.90%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN LRFLCGHFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARAKR+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]

BLAST of CmoCh04G001160 vs. ExPASy TrEMBL
Match: A0A1S3C4Y1 (staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.7e-167
Identity = 291/338 (86.09%), Postives = 314/338 (92.90%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN LRFLCGHFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARAKR+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]

BLAST of CmoCh04G001160 vs. NCBI nr
Match: XP_022941859.1 (staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphylococcal-like nuclease CAN2 [Cucurbita pepo subsp. pepo] >KAG6599960.1 Staphylococcal-like nuclease CAN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030638.1 Staphylococcal-like nuclease CAN2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 682.6 bits (1760), Expect = 1.8e-192
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmoCh04G001160 vs. NCBI nr
Match: XP_022996106.1 (staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococcal-like nuclease CAN2 [Cucurbita maxima])

HSP 1 Score: 674.9 bits (1740), Expect = 3.7e-190
Identity = 332/337 (98.52%), Postives = 334/337 (99.11%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN LRFLC HFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNALRFLCSHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQ NWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH
Sbjct: 61  VSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAP+PTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN
Sbjct: 121 TLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY
Sbjct: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR
Sbjct: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           AELAKWENEARA+RIGLWASRNPEQPWEWRKGKREGK
Sbjct: 301 AELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 337

[Close]

BLAST of CmoCh04G001160 vs. NCBI nr
Match: XP_038892991.1 (staphylococcal-like nuclease CAN1 isoform X1 [Benincasa hispida])

HSP 1 Score: 617.8 bits (1592), Expect = 5.4e-173
Identity = 306/337 (90.80%), Postives = 316/337 (93.77%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGNVL FLCGHFCNP+    +DS  PHGVS AT GVSALAHDLFDFEITSQVPQGLSKHV
Sbjct: 1   MGNVLGFLCGHFCNPS----SDSQPPHGVSYATAGVSALAHDLFDFEITSQVPQGLSKHV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSSKKAQANWYRKLA+AW+ EKPPP+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLPH
Sbjct: 61  VSSKKAQANWYRKLADAWKLEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVELSAPAP   SLPDGVKFEL+TLPVDAKAVADGDTVTVYVSTAH RESSCIP+EVRN
Sbjct: 121 TLVELSAPAPTTASLPDGVKFELETLPVDAKAVADGDTVTVYVSTAHTRESSCIPIEVRN 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AAIQRSQARNAKN+AKADALHKTITDAGYRVLT QNEEILARKYRIRLRGIDAPES MPY
Sbjct: 181 AAIQRSQARNAKNFAKADALHKTITDAGYRVLTFQNEEILARKYRIRLRGIDAPESGMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEAK+EL KLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY AYDKR
Sbjct: 241 GKEAKQELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYKAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           AEL+KWENEARAKR GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 AELSKWENEARAKRAGLWASSNPEQPWEWRKGKREGK 333

[Close]

BLAST of CmoCh04G001160 vs. NCBI nr
Match: XP_022146824.1 (staphylococcal-like nuclease CAN2 [Momordica charantia])

HSP 1 Score: 606.3 bits (1562), Expect = 1.6e-169
Identity = 303/343 (88.34%), Postives = 315/343 (91.84%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAA-----DADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60
           MGN LRFLCGHFC+P+A +      +DSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG
Sbjct: 1   MGNALRFLCGHFCSPSAGSGGGGGGSDSLGPHGVSPATVGVSALAHDLFDFEITSQVPQG 60

Query: 61  LSKHVVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120
           LSKHVVSSKKAQANWY KLAEAWRSEKPPP+TPEEASRLVIQTLKRHQKKDVEGLLAFYG
Sbjct: 61  LSKHVVSSKKAQANWYGKLAEAWRSEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLAFYG 120

Query: 121 LPLPHTLVELSAPAPVP-TSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCI 180
           LPLPHTLVELSA AP     LPDG+KFELQTLPVDAKAVADGDTVTVYVST +PRES+CI
Sbjct: 121 LPLPHTLVELSASAPTTGAPLPDGIKFELQTLPVDAKAVADGDTVTVYVSTENPRESACI 180

Query: 181 PMEVRNAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAP 240
           P EV+ AA+QRS AR AKNYAKADALHK ITDAGYRVLT QNEEILA+KYRIRLRGIDAP
Sbjct: 181 PKEVQAAAVQRSHARAAKNYAKADALHKKITDAGYRVLTFQNEEILAKKYRIRLRGIDAP 240

Query: 241 ESAMPYGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300
           ES+MPYGKEAKEEL KLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY
Sbjct: 241 ESSMPYGKEAKEELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY 300

Query: 301 TAYDKRAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
            AYDKRAELAKWE EARAKR GLWAS NPEQPWEWRK KREGK
Sbjct: 301 AAYDKRAELAKWEKEARAKRAGLWASSNPEQPWEWRKAKREGK 343

[Close]

BLAST of CmoCh04G001160 vs. NCBI nr
Match: XP_008456902.1 (PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo] >TYK25847.1 staphylococcal-like nuclease CAN1 [Cucumis melo var. makuwa])

HSP 1 Score: 597.0 bits (1538), Expect = 9.8e-167
Identity = 291/338 (86.09%), Postives = 314/338 (92.90%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60
           MGN LRFLCGHFC+P     +DSL PH  +S +T GVSALAHDLF+FEITSQVP GL KH
Sbjct: 1   MGNALRFLCGHFCHPT----SDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKH 60

Query: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120
           VVSSKKAQANWY KLAEAW+SEKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120

Query: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180
           HTLV+ SAPAP   S+PDGVKFELQTLPVDAKAVADGDTVTV+VST+HPRESSC+P EVR
Sbjct: 121 HTLVKSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVR 180

Query: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 338
           R EL+KWENEARAKR+GLWAS NP++PWEWRKGKREGK
Sbjct: 301 RPELSKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]

BLAST of CmoCh04G001160 vs. TAIR 10
Match: AT2G40410.2 (Staphylococcal nuclease homologue )

HSP 1 Score: 477.2 bits (1227), Expect = 1.1e-134
Identity = 237/334 (70.96%), Postives = 272/334 (81.44%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN L FL G  C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQANWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  APV  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLRGIDAPES MP+
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPF 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA+E L K+V  K L+VLVYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKR
Sbjct: 241 GKEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKR 335
             LAKWE EAR KRIGLWAS NPE+PW+WRK  R
Sbjct: 301 PVLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

[Close]

BLAST of CmoCh04G001160 vs. TAIR 10
Match: AT3G56170.1 (Ca-2+ dependent nuclease )

HSP 1 Score: 455.3 bits (1170), Expect = 4.3e-128
Identity = 226/336 (67.26%), Postives = 263/336 (78.27%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN +R L               L  HGVS ++ GVSAL+ DL +FE TSQVP+ L  +V
Sbjct: 1   MGNAIRLL------------RKCLNSHGVSASSGGVSALSRDLLNFETTSQVPEKLGSYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
           VSS+KAQANWYRK+ EAW+  KP PKTPEEASRLVI  LK HQK DVEGLL+FYGLP PH
Sbjct: 61  VSSQKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
            LVE+  P   P SLP GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +P +V  
Sbjct: 121 NLVEV--PTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMPY 240
           AA++R++AR  KNY +ADALHKTI  +GYR+++ QNEE+LA+K+RIRL GID+PES MPY
Sbjct: 181 AAVKRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPY 240

Query: 241 GKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKR 300
           GKEA +EL K+VEGKCL+VLVY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR
Sbjct: 241 GKEAHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKR 300

Query: 301 AELAKWENEARAKRIGLWASRNPEQPWEWRKGKREG 337
           AELAKWENEAR KR+GLWAS NPE+PWEWRK KR G
Sbjct: 301 AELAKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

[Close]

BLAST of CmoCh04G001160 vs. TAIR 10
Match: AT2G40410.1 (Staphylococcal nuclease homologue )

HSP 1 Score: 307.0 bits (785), Expect = 1.9e-83
Identity = 157/229 (68.56%), Postives = 184/229 (80.35%), Query Frame = 0


Query: 1   MGNVLRFLCGHFCNPNAAADADSLGPHGVSPATVGVSALAHDLFDFEITSQVPQGLSKHV 60
           MGN L FL G  C P      DSLGPHGVS ATVGVSALAHDLF+FEITSQVP+GL ++V
Sbjct: 1   MGNALTFLYGKCCKPTTT--DDSLGPHGVSAATVGVSALAHDLFNFEITSQVPEGLGRYV 60

Query: 61  VSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLPH 120
            SS+KAQANWYRK+ EAW+  KPPP+T EEASRLV   LKR+QK DVEGLL+FYGLPL H
Sbjct: 61  QSSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSH 120

Query: 121 TLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVRN 180
           TLVE++  APV  SLP+G+ FE QTLPVD KAVADGDT+TVYVST+ P  SS +P EV  
Sbjct: 121 TLVEVTVEAPV--SLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNL 180

Query: 181 AAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLR 230
           AA+QR++AR  +NY KAD LH+ I D+GYRVL ++NEE+LARK+RIRLR
Sbjct: 181 AAVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLR 225

The following BLAST results are available for this feature:

BLAST of CmoCh04G001160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4IH31	1.5e-133	70.96	Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 S...	[more]
F4IZC5	6.0e-127	67.26	Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 S...	[more]
Q39635	1.8e-126	67.16	Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1	[more]
Q942N7	9.7e-117	62.43	Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q2RBJ2	2.0e-98	53.56	Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of CmoCh04G001160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FPN5	8.6e-193	100.00	staphylococcal-like nuclease CAN2 OS=Cucurbita moschata OX=3662 GN=LOC111447091 ...	[more]
A0A6J1K113	1.8e-190	98.52	staphylococcal-like nuclease CAN2 OS=Cucurbita maxima OX=3661 GN=LOC111491421 PE...	[more]
A0A6J1CZ71	7.8e-170	88.34	staphylococcal-like nuclease CAN2 OS=Momordica charantia OX=3673 GN=LOC111015935...	[more]
A0A5D3DQ96	4.7e-167	86.09	Staphylococcal-like nuclease CAN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3C4Y1	4.7e-167	86.09	staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 S...	[more]

BLAST of CmoCh04G001160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941859.1	1.8e-192	100.00	staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphyloc...	[more]
XP_022996106.1	3.7e-190	98.52	staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococ...	[more]
XP_038892991.1	5.4e-173	90.80	staphylococcal-like nuclease CAN1 isoform X1 [Benincasa hispida]	[more]
XP_022146824.1	1.6e-169	88.34	staphylococcal-like nuclease CAN2 [Momordica charantia]	[more]
XP_008456902.1	9.8e-167	86.09	PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo] >TYK25847.1 staphylo...	[more]

BLAST of CmoCh04G001160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40410.2	1.1e-134	70.96	Staphylococcal nuclease homologue	[more]
AT3G56170.1	4.3e-128	67.26	Ca-2+ dependent nuclease	[more]
AT2G40410.1	1.9e-83	68.56	Staphylococcal nuclease homologue	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016071	Staphylococcal nuclease (SNase-like), OB-fold	SMART	SM00318	SNASE_2	coord: 202..320 e-value: 9.0E-33 score: 124.9
IPR016071	Staphylococcal nuclease (SNase-like), OB-fold	PFAM	PF00565	SNase	coord: 225..319 e-value: 8.1E-28 score: 97.1
IPR016071	Staphylococcal nuclease (SNase-like), OB-fold	PROSITE	PS50830	TNASE_3	coord: 144..320 score: 31.643545
IPR035437	SNase-like, OB-fold superfamily	GENE3D	2.40.50.90		coord: 141..324 e-value: 4.3E-31 score: 109.8
None	No IPR available	PANTHER	PTHR12302:SF21	STAPHYLOCOCCAL-LIKE NUCLEASE CAN2	coord: 15..332
None	No IPR available	PANTHER	PTHR12302	EBNA2 BINDING PROTEIN P100	coord: 15..332
None	No IPR available	SUPERFAMILY	50199	Staphylococcal nuclease	coord: 146..327

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G001160.1	CmoCh04G001160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004518	nuclease activity
molecular_function	GO:0003676	nucleic acid binding